Faculty Profiles - MURAYAMA Keiji

Papers - MURAYAMA Keiji

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Nonenzymatic polymerase-like template-directed synthesis of acyclic l-threoninol nucleic acid Reviewed

Murayama Keiji, Okita Hikari, Kuriki Takumi, Asanuma Hiroyuki

NATURE COMMUNICATIONS Vol. 12 ( 1 ) 2021.2

Reversible Optical Control of Receptor Tyrosine Kinase Activity and ERK Dynamics Using Azobenzene-Carrying DNA Aptamer Agonist

Masahiro Wakano, Masaya Tsunoda, Keiji Murayama, Jumpei Morimoto, Ryosuke Ueki, Saeko Aoyama-Ishiwatari, Yusuke Hirabayashi, Hiroyuki Asanuma, Shinsuke Sando

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY Vol. 147 ( 13 ) page： 11477 - 11484 2025.3

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER CHEMICAL SOC

Receptor tyrosine kinases (RTKs) play a pivotal role in cell signaling through their activation via dimerization. Recent studies have demonstrated the importance of the temporal dynamics of RTK activity and downstream signals, such as ERK, in determining the cell fate. To better understand these dynamics, it is essential to develop methods capable of controlling the RTK activity with high temporal resolution. However, techniques for precisely modulating the activity of endogenous RTKs without requiring genetic modification remain insufficiently established. In this study, we developed a DNA aptamer agonist, Met-azo-aptamer, which enables reversible optical control of the activity of the c-Met receptor, a member of the RTK family. This was achieved by incorporating azobenzene, a photoisomerizable molecule, into a DNA aptamer that binds to c-Met. This design allows light-induced switching between the active and inactive structures of the aptamer. When the aptamer was applied to HeLa cells and exposed to ultraviolet or blue light, phosphorylation signals within the cells were activated in response to the light patterns. Furthermore, by variation of the light patterns, the Met-azo-aptamer successfully controlled the timing, amplitude, and duration of downstream ERK activation. The Met-azo-aptamer developed in this study offers a high-resolution method for investigating the relationship between RTK activation patterns and cell function or fate.

DOI： 10.1021/jacs.5c01559

Web of Science

PubMed

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A Light-Powered Single-Stranded DNA Molecular Motor with Colour-Selective Single-Step Control Reviewed International coauthorship

Anderson, T; Wu, W; Sirbu, O; Tong, KS; Siti, W; Liu, XR; Kou, B; Murayama, K; Asanuma, H; Wang, ZS

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION Vol. 63 ( 32 ) 2024.8

DNA Reaction System That Acquires Classical Conditioning Reviewed International coauthorship

Nakakuki, T; Toyonari, M; Aso, K; Murayama, K; Asanuma, H; de Greef, TFA

ACS SYNTHETIC BIOLOGY Vol. 13 ( 2 ) page： 521 - 529 2024.1

Highly Functional Acyclic Xeno Nucleic Acids Invited Reviewed

Murayama, K; Okita, H; Asanuma, H

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN Vol. 96 ( 10 ) page： 1179 - 1187 2023.10

Rapid Chemical Ligation of DNA and Acyclic Threoninol Nucleic Acid (aTNA) for Effective Nonenzymatic Primer Extension Reviewed

Hikari Okita, Shuto Kondo, Keiji Murayama, Hiroyuki Asanuma

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY Vol. 145 ( 32 ) page： 17872 - 17880 2023.7

Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：AMER CHEMICAL SOC

Previously, nonenzymatic primer extension reaction of acyclic l-threoninol nucleic acid (L-aTNA) wasachieved in the presence of N-cyanoimidazole (CNIm)and Mn2+; however, the reaction conditions were not optimizedand a mechanistic insight was not sufficient. Herein, we report investigationof the kinetics and reaction mechanism of the chemical ligation ofL-aTNA to L-aTNA and of DNA to DNA.We found that Cd2+, Ni2+, and Co2+ accelerated ligation of both L-aTNA and DNA and that the rate-determining step was activationof the phosphate group. The activation was enhanced by duplex formationbetween a phosphorylated L-aTNA fragment and template,resulting in unexpectedly more effective L-aTNA ligationthan DNA ligation. Under optimized conditions, an 8-mer L-aTNA primer could be elongated by ligation to L-aTNA trimers to produce a 29-mer full-length oligomer with60% yield within 2 h at 4 & DEG;C. This highly effective chemicalligation system will allow construction of artificial genomes, robustDNA nanostructures, and xeno nucleic acids for use in selection methods.Our findings also shed light on the possible pre-RNA world.

DOI： 10.1021/jacs.3c04979

Web of Science

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Functionalization of acyclic xenonucleic acid with modified nucleobases Invited Reviewed

Keiji Murayama, Yuuhei Yamano, Hiroyuki Asanuma

Polymer Journal Vol. 55 ( 7 ) page： 743 - 752 2023.7

Kinesin motors driven microtubule swarming triggered by UV light Reviewed

Ishii Satsuki, Akter Mousumi, Murayama Keiji, Kabir Arif Md Rashedul, Asanuma Hiroyuki, Sada Kazuki, Kakugo Akira

POLYMER JOURNAL Vol. 54 ( 12 ) page： 1501 - 1507 2022.12

DNA Concentration Regulator That can be Driven for a Long Time Reviewed

Nakakuki Takashi, Murayama Keiji, Asanuma Hiroyuki

NEW GENERATION COMPUTING Vol. 40 ( 2 ) page： 681 - 702 2022.7

Orthogonal Amplification Circuits Composed of Acyclic NucleicAcids Enable RNA Detection br Reviewed

Chen Yanglingzhi, Nagao Ryuya, Murayama Keiji, Asanuma Hiroyuki

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY Vol. 144 ( 13 ) page： 5887 - 5892 2022.4

Methyl group configuration on acyclic threoninol nucleic acids (aTNAs) impacts supramolecular properties Reviewed

Murayama Keiji, Kashida Hiromu, Asanuma Hiroyuki

ORGANIC & BIOMOLECULAR CHEMISTRY 2022.3

Unexpected Dissociation of Photoresponsive UV-ON DNA Carrying p-tert-Butyl Azobenzene under UV Light Irradiation Reviewed

Ishii Satsuki, Murayama Keiji, Sada Kazuki, Asanuma Hiroyuki, Kakugo Akira

CHEMISTRY LETTERS Vol. 51 ( 3 ) page： 292 - 295 2022.3

Signal Amplification Circuit Composed of Serinol Nucleic Acid for RNA Detection Reviewed

Chen Yanglingzhi, Murayama Keiji, Asanuma Hiroyuki

CHEMISTRY LETTERS Vol. 51 ( 3 ) page： 330 - 333 2022.3

Renewable DNA Proportional-Integral Controller with Photoresponsive Molecules

Tamba Masaaki, Murayama Keiji, Asanuma Hiroyuki, Nakakuki Takashi

MICROMACHINES Vol. 13 ( 2 ) 2022.2

Xeno nucleic acids (XNAs) having non-ribose scaffolds with unique supramolecular properties Invited Reviewed

Asanuma Hiroyuki, Kamiya Yukiko, Kashida Hiromu, Murayama Keiji

CHEMICAL COMMUNICATIONS 2022.1

A Pyrene-Modified Serinol Nucleic Acid Nanostructure Converts the Chirality of Threoninol Nucleic Acids into Circularly Polarized Luminescence Signals Reviewed

Kashida Hiromu, Nishikawa Keiji, Ito Yuka, Murayama Keiji, Hayashi Ichiyo, Kakuta Takahiro, Ogoshi Tomoki, Asanuma Hiroyuki

CHEMISTRY-A EUROPEAN JOURNAL Vol. 27 ( 59 ) page： 14582 - 14585 2021.10

RNA以前に非環状型XNAがPre-RNAとして存在したか? Invited

浅沼浩之, 沖田ひかり, 村山恵司

Vol. 49 page： 9 2021.9

Design and Hybridization Properties of Acyclic Xeno Nucleic Acid Oligomers Reviewed

Murayama Keiji, Asanuma Hiroyuki

CHEMBIOCHEM Vol. 22 ( 15 ) page： 2507 - 2515 2021.8

Dual Crosslinking Photo-Switches for Orthogonal Photo-Control of Hybridization Between Serinol Nucleic Acid and RNA Reviewed

Yamano Yuuhei, Murayama Keiji, Asanuma Hiroyuki

Dual Crosslinking Photo-Switches for Orthogonal Photo-Control of Hybridization Between Serinol Nucleic Acid and RNA Vol. 27 ( 14 ) page： 4599 - 4604 2021.3

Light-Regulated Liquid-Liquid Phase Separation for Spatiotemporal Protein Recruitment and Cell Aggregation Reviewed

Light-Regulated Liquid-Liquid Phase Separation for Spatiotemporal Protein Recruitment and Cell Aggregation

Light-Regulated Liquid-Liquid Phase Separation for Spatiotemporal Protein Recruitment and Cell Aggregation Vol. 13 ( 4 ) page： 5652 - 5659 2021.2

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Papers - MURAYAMA Keiji