研究者詳細 - 木村　康明

論文 - 木村　康明

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Nearest-neighbor parameter for inosine-cytosine pairs through a combined experimental and computational approach 査読有り国際誌

Shun Sakuraba, Junichi Iwakiri, Michiaki Hamada, Tomoshi Kameda, Genichiro Tsuji, Yasuaki Kimura, Hiroshi Abe, Kiyoshi Asai

2018年10月

Chemical ligation reaction for oligonucleotides based on electrophilic phosphorothioester

Kimura Yasuaki, Maruyama Hideto, Oikawa Ryota, Hayakawa Mayu, Abe Naoko, Tsuji Genichiro, Matsuda Akira, Shuto Satoshi, Ito Yoshihiro, Abe Hiroshi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 255 巻頁： . 2018年3月

Development of glutathione S-transferase (GST) covalent inhibitor

Shishido, Y; Tomoike, F; Kimura, Y; Kuwata, K; Yano, T; Fukui, K; Fujikawa, H; Sekido, Y; Murakami-Tonami, Y; Kameda, T; Shuto, S; Abe, H

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 255 巻頁： . 2018年3月

Chemical ligation reaction for oligonucleotides based on electrophilic phosphorothioester 査読有り国際誌

Kimura Yasuaki, Maruyama Hideto, Oikawa Ryota, Hayakawa Mayu, Abe Naoko, Tsuji Genichiro, Matsuda Akira, Shuto Satoshi, Ito Yoshihiro, Abe Hiroshi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 255 巻 2018年3月

Chemical Ligation Reactions of Oligonucleotides for Biological and Medicinal Applications

Abe Hiroshi, Kimura Yasuaki

CHEMICAL & PHARMACEUTICAL BULLETIN 66 巻 ( 2 ) 頁： 117-122 2018年2月

Chemical Ligation Reactions of Oligonucleotides for Biological and Medicinal Applications. 査読有り国際誌

Abe H, Kimura Y

Chemical & pharmaceutical bulletin 66 巻 ( 2 ) 頁： 117 - 122 2018年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemical and Pharmaceutical Bulletin

Chemical ligation of oligonucleotides (ONs) is the key reaction for various ON-based technologies. We have tried to solve the problems of RNA interference (RNAi) technology by applying ON chemical ligation to RNAi. We designed a new RNAi system, called intracellular buildup RNAi (IBR-RNAi), where the RNA fragments are built up into active small-interference RNA (siRNA) in cells through a chemical ligation reaction. Using the phosphorothioate and iodoacetyl groups as reactive functional groups for the ligation, we achieved RNAi effects without inducing immune responses. Additionally, we developed a new chemical ligation for IBR-RNAi, which affords a more native-like structure in the ligated product. The new ligation method should be useful not only for IBR-RNAi but also for the chemical synthesis of biofunctional ONs.

DOI： 10.1248/cpb.c17-00615

A covalent G-site inhibitor for glutathione S-transferase Pi (GSTP(1-1)). 査読有り国際誌

Shishido Y, Tomoike F, Kimura Y, Kuwata K, Yano T, Fukui K, Fujikawa H, Sekido Y, Murakami-Tonami Y, Kameda T, Shuto S, Abe H

Chemical communications (Cambridge, England) 53 巻 ( 81 ) 頁： 11138 - 11141 2017年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemical Communications

We herein report the first covalent G-site-binding inhibitor for GST, GS-ESF (1), which irreversibly inhibited the GSTP1-1 function. LC-MS/MS and X-ray structure analyses of the covalently linked GST-inhibitor complex suggested that 1 reacted with Tyr108 of GSTP1-1. The mechanism of covalent bond formation was discussed based on MD simulation results.

DOI： 10.1039/c7cc05829b

Chemical ligation of oligonucleotides using an electrophilic phosphorothioester. 査読有り国際誌 Open Access

Maruyama H, Oikawa R, Hayakawa M, Takamori S, Kimura Y, Abe N, Tsuji G, Matsuda A, Shuto S, Ito Y, Abe H

Nucleic acids research 45 巻 ( 12 ) 頁： 7042 - 7048 2017年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Nucleic Acids Research

We developed a new approach for chemical ligation of oligonucleotides using the electrophilic phosphorothioester (EPT) group. A nucleophilic phosphorothioate group on oligonucleotides was converted into the EPT group by treatment with Sanger's reagent (1-fluoro-2,4-dinitrobenzene). EPT oligonucleotides can be isolated, stored frozen, and used for the ligation reaction. The reaction of the EPT oligonucleotide and an amino-modified oligonucleotide took place without any extra reagents at pH 7.0-8.0 at room temperature, and resulted in a ligation product with a phosphoramidate bond with a 39-85% yield. Thismethod has potential uses in biotechnology and chemical biology.

DOI： 10.1093/nar/gkx459

Supramolecular Ligands for Histone Tails by Employing a Multivalent Display of Trisulfonated Calix[4]arenes. 査読有り

Kimura Y, Saito N, Hanada K, Liu J, Okabe T, Kawashima SA, Yamatsugu K, Kanai M

Chembiochem : a European journal of chemical biology 16 巻 ( 18 ) 頁： 2599 - 2604 2015年12月

Catalytic anomeric aminoalkynylation of unprotected aldoses. 査読有り

Kimura Y, Ito S, Shimizu Y, Kanai M

Organic letters 15 巻 ( 16 ) 頁： 4130 - 4133 2013年8月

Inhibition of MAO-A and stimulation of behavioural activities in mice by the inactive prodrug form of the anti-influenza agent oseltamivir. 査読有り

Hiasa M, Isoda Y, Kishimoto Y, Saitoh K, Kimura Y, Kanai M, Shibasaki M, Hatakeyama D, Kirino Y, Kuzuhara T

British journal of pharmacology 169 巻 ( 1 ) 頁： 115 - 129 2013年5月

Design and Synthesis of Resin-Conjugated Tamiflu Analogs for Affinity Chromatography 査読有り

Yasuaki Kimura, Kenzo Yamatsugu, Motomu Kanai, Noriko Echigo, Takashi Kuzuhara, Masakatsu Shibasaki

BULLETIN OF THE KOREAN CHEMICAL SOCIETY 31 巻 ( 3 ) 頁： 588 - 594 2010年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：KOREAN CHEMICAL SOC

Two types of resin-conjugated Tamiflu analogs were synthesized by modifying our original synthetic route of oseltamivir phosphate (Tamiflu). The prepared resins bound to influenza virus neuraminidase, the main target of Tamiflu. The resins will be useful for isolating and identifying presumed endogenous vertebrate proteins that interact with Tamiflu, which might relate to the rarely observed abnormal behavior exhibited by some influenza patients treated with Tamiflu.

DOI： 10.5012/bkcs.2010.31.03.588

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Design and synthesis of immobilized Tamiflu analog on resin for affinity chromatography 査読有り

Yasuaki Kimura, Kenzo Yamatsugu, Motomu Kanai, Noriko Echigo, Takashi Kuzuhara, Masakatsu Shibasaki

TETRAHEDRON LETTERS 50 巻 ( 26 ) 頁： 3205 - 3208 2009年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD

A resin linked with the Tamiflu core was synthesized by modifying our original synthetic route Of oseltamivir phosphate (Tamiflu). The prepared resin bound to the influenza virus enzyme neuraminidase, the main target of Tamiflu. The immobilized Tamiflu analog will be useful for isolating and identifying presumed endogenous vertebrate proteins that interact with Tamiflu, which might relate to the abnormal behavior exhibited by some influenza patients treated with Tamiflu. (C) 2009 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.tetlet.2009.01.142

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A synthesis of Tamiflu by using a barium-catalyzed asymmetric Diels-Alder-type reaction. 査読有り

Yamatsugu K, Yin L, Kamijo S, Kimura Y, Kanai M, Shibasaki M

Angewandte Chemie (International ed. in English) 48 巻 ( 6 ) 頁： 1070 - 1076 2009年

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論文 - 木村　康明