記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/s41598-017-05327-8

Web of Science

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.cmet.2016.12.002

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

The anxiolytic diazepam selectively inhibits psychological stress-induced autonomic and behavioral responses without causing noticeable suppression of other central performances. This pharmacological property of diazepam led us to the idea that neurons that exhibit diazepam-sensitive, psychological stress-induced activation are potentially those recruited for stress responses. To obtain neuroanatomical clues for the central stress circuitries, we examined the effects of diazepam on psychological stress-induced neuronal activation in broad brain regions. Rats were exposed to a social defeat stress, which caused an abrupt increase in body temperature by up to 2 C. Pretreatment with diazepam (4 mg/kg, i.p.) attenuated the stress-induced hyperthermia, confirming an inhibitory physiological effect of diazepam on the autonomic stress response. Subsequently, the distribution of cells expressing Fos, a marker of neuronal activation, was examined in 113 forebrain and midbrain regions of these rats after the stress exposure and diazepam treatment. The stress following vehicle treatment markedly increased Fos-immunoreactive (IR) cells in most regions of the cerebral cortex, limbic system, thalamus, hypothalamus and midbrain, which included parts of the autonomic, neuroendocrine, emotional and arousal systems. The diazepam treatment significantly reduced the stress-induced Fos expression in many brain regions including the prefrontal, sensory and motor cortices, septum, medial amygdaloid nucleus, medial and lateral preoptic areas, parvicellular paraventricular hypothalamic nucleus, dorsomedial hypothalamus, perifornical nucleus, tuberomammillary nucleus, association, midline and intralaminar thalami, and median and dorsal raphe nuclei. In contrast, diazepam increased Fos-IR cells in the central amygdaloid nucleus, medial habenular nucleus, ventromedial hypothalamic nucleus and magnocellular lateral hypothalamus. These results provide important information for elucidating the neural circuitries that mediate the autonomic and behavioral responses to psychosocial stressors. (C) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.neuroscience.2014.04.047

Web of Science

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1152/ajpheart.00262.2011

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/j.1460-9568.2011.07863.x

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.neuroscience.2009.03.041

Web of Science

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SOC NEUROSCIENCE  

Sympathetic premotor neurons directly control sympathetic preganglionic neurons (SPNs) in the intermediolateral cell column (IML) of the thoracic spinal cord, and many of these premotor neurons are localized in the medulla oblongata. The rostral ventrolateral medulla contains premotor neurons controlling the cardiovascular conditions, whereas rostral medullary raphe regions are a candidate source of sympathetic premotor neurons for thermoregulatory functions. Here, we show that these medullary raphe regions contain putative glutamatergic neurons and that these neurons directly control thermoregulatory SPNs. Neurons expressing vesicular glutamate transporter 3 (VGLUT3) were distributed in the rat medullary raphe regions, including the raphe magnus and rostral raphe pallidus nuclei, and mostly lacked serotonin immunoreactivity. These VGLUT3-positive neurons expressed Fos in response to cold exposure or to central administration of prostaglandin E-2, a pyrogenic mediator. Transneuronal retrograde labeling after inoculation of pseudorabies virus into the interscapular brown adipose tissue (BAT) or the tail indicated that those VGLUT3-expressing medullary raphe neurons innervated these thermoregulatory effector organs multisynaptically through SPNs of specific thoracic segments, and microinjection of glutamate into the IML of the BAT-controlling segments produced BAT thermogenesis. An anterograde tracing study further showed a direct projection of those VGLUT3-expressing medullary raphe neurons to the dendrites of SPNs. Furthermore, intra-IML application of glutamate receptor antagonists blocked BAT thermogenesis triggered by disinhibition of the medullary raphe regions. The present results suggest that VGLUT3-expressing neurons in the medullary raphe regions constitute excitatory neurons that could be categorized as a novel group of sympathetic premotor neurons for thermoregulatory functions, including fever.

DOI： 10.1523/JNEUROSCI.1219-04.2004

Web of Science

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（その他）  

担当区分：筆頭著者,　責任著者   掲載種別：記事・総説・解説・論説等（学術雑誌）  

担当区分：筆頭著者,　責任著者   掲載種別：記事・総説・解説・論説等（その他）  

記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：FEDERATION AMER SOC EXP BIOL  

Web of Science

担当区分：筆頭著者   記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）  

記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：FEDERATION AMER SOC EXP BIOL  

Web of Science

記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：日本生理学会  

The rostral ventromedial medulla (RVMM) sympathetically regulates thermogenesis in brown adipose tissue (BAT) and constriction of skin blood vessels. However, the sympathetic-related neurons in the RVMM remain to be identified. First, we found that vesicular glutamate transporter 3 (VGLUT3) -immunoreactive neurons in the rostral raphe pallidus nucleus and raphe magnus nucleus expressed Fos in response to both fever-inducing stimulus (central injection of prostaglandin E<SUB>2</SUB>) and cold-exposure (4°C, 4 hours) in rats. Most of the VGLUT3-expressing neurons were negative for serotonin immunoreactivity, and many of them were labeled trans-synaptically with pseudorabies virus inoculated into rat interscapular BAT or tail. Furthermore, we found that the VGLUT3-positive neurons directly project onto sympathetic preganglionic neurons in the intermediolateral cell column (IML) of the spinal cord; and that VGLUT3-positive axon terminals in the IML formed asymmetric synapses, suggesting the excitatory characteristics of the synapses. These results suggest that the rostral medullary raphe regions contain glutamatergic neurons that could be categorized as a novel group of sympathetic premotor neurons involved in fever induction and thermoregulation. <b>[Jpn J Physiol 54 Suppl:S196 (2004)]</b>

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会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

開催年月日： 2005年

記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）