2021/04/02 更新

写真a

チバ ソウタロウ
千葉 壮太郎
CHIBA Sotaro
所属
大学院生命農学研究科 植物生産科学専攻 植物生産科学 准教授
職名
准教授
外部リンク

学位 1

  1. 博士(学術) ( 2008年3月   岡山大学 ) 

研究キーワード 5

  1. ヴァイロコントロール

  2. 植物保護

  3. 菌類ウイルス

  4. 植物ウイルス

  5. ファイトプラズマ

研究分野 3

  1. ライフサイエンス / ウイルス学

  2. ライフサイエンス / 応用分子細胞生物学

  3. 環境・農学 / 植物保護科学

現在の研究課題とSDGs 4

  1. 菌類ウイルスの遺伝子発現機構

  2. ウイルスを用いた植物病原糸状菌防除

  3. 東南アジアの病害虫防除

  4. 植物ウイルスの宿主抵抗性打破

経歴 3

  1. 名古屋大学   大学院生命農学研究科   准教授

    2019年 - 現在

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    国名:日本国

  2. 名古屋大学   アジアサテライトキャンパス学院   特任准教授

    2015年 - 2018年

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    国名:日本国

  3. 岡山大学   資源植物科学研究科   博士研究員・特別契約職員助教

    2008年 - 2014年

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    国名:日本国

学歴 3

  1. 岡山大学   自然科学研究科

    2002年4月 - 2008年3月

  2. 岡山大学   自然科学研究科

    1999年4月 - 2002年3月

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    国名: 日本国

  3. 岡山理科大学   理学部   生物化学科

    1995年4月 - 1999年3月

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    国名: 日本国

所属学協会 2

  1. 日本植物病理学会

  2. 日本ウイルス学会

 

論文 53

  1. Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range 査読有り

    VIROLOGY   554 巻   頁: 55 - 65   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.virol.2020.11.011

    Web of Science

  2. Geographic Distribution, Genetic Variability and Biological Properties of Rice Orange Leaf Phytoplasma in Southeast Asia 査読有り 国際共著

    Ong S, Jonson GB, Calassanzio M, Rin S, Chou C, Oi T, Chiba S.

    PATHOGENS   10 巻 ( 2 ) 頁: 169   2021年2月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/pathogens10020169

  3. Reemerging Rice Orange Leaf Phytoplasma with Varying Symptoms Expressions and Its Transmission by a New Leafhopper Vector-Nephotettix virescens Distant 査読有り 国際共著

    Jonson Gilda B., Matres Jerlie M., Ong Socheath, Tanaka Toshiharu, Choi Il-Ryong, Chiba Sotaro

    PATHOGENS   9 巻 ( 12 ) 頁: 990   2020年11月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/pathogens9120990

    Web of Science

  4. Pathogenetic roles of beet necrotic yellow vein virus RNA5 in the exacerbation of symptoms and yield reduction, development of scab-like symptoms, andRz1-resistance breaking in sugar beet 査読有り 国際共著

    Tamada Tetsuo, Uchino Hirokatsu, Kusume Toshimi, Iketani-Saito Minako, Chiba Sotaro, Andika Ida Bagus, Kondo Hideki

    PLANT PATHOLOGY   70 巻 ( 1 ) 頁: 219 - 232   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/ppa.13266

    Web of Science

  5. Expression profiles of genes for enzymes involved in capsidiol production inNicotiana benthamiana 査読有り

    Rin Soriya, Imano Sayaka, Camagna Maurizio, Suzuki Takamasa, Tanaka Aiko, Sato Ikuo, Chiba Sotaro, Kawakita Kazuhito, Takemoto Daigo

    JOURNAL OF GENERAL PLANT PATHOLOGY   86 巻 ( 5 ) 頁: 340 - 349   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s10327-020-00931-5

    Web of Science

  6. Rice blast management in Cambodian rice fields using Trichoderma harzianum and a resistant variety 査読有り 国際共著

    Chou Cheythyrith, Castilla Nancy, Hadi Buyung, Tanaka Toshiharu, Chiba Sotaro, Sato Ikuo

    CROP PROTECTION   135 巻   頁: 104864   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.cropro.2019.104864

    Web of Science

  7. In Vitro Assessment of Biocontrol Effects on Fusarium Head Blight and Deoxynivalenol (DON) Accumulation by DON-Degrading Bacteria 査読有り

    Morimura Hiroyuki, Ito Michihiro, Yoshida Shigenobu, Koitabashi Motoo, Tsushima Seiya, Camagna Maurizio, Chiba Sotaro, Takemoto Daigo, Kawakita Kazuhito, Sato Ikuo

    TOXINS   12 巻 ( 6 ) 頁: 399   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/toxins12060399

    Web of Science

  8. BcCPDH1 is involved in the detoxification of sesquiterpenoid phytoalexin capsidiol, and specifically required for the pathogenicity of Botrytis cinerea on Nicotiana species

    Kuroyanagi T., Ojika M., Suzuki T., Sato I., Chiba S., Kawakita K., Takemoto D.

    MOLECULAR PLANT-MICROBE INTERACTIONS   32 巻 ( 10 ) 頁: 40-40   2019年10月

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    記述言語:英語   掲載種別:研究論文(研究会,シンポジウム資料等)  

    Web of Science

  9. Mycoviruses infecting Fusarium species isolated from Ethiopian wheat fields and their potential association with hypovirulece of a Fusarium Head Bright fungus 国際共著

    Mizutani Y., Adane A., Uesaka K., Kondo H., Suga H., Suzuki N., Chiba S.

    MOLECULAR PLANT-MICROBE INTERACTIONS   32 巻 ( 10 ) 頁: 24-25   2019年10月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    Web of Science

  10. Solanaceae-specific secretory peptide SAR8.2m is essential for non-host resistance of Nicotiana benthamiana to a variety of taxonomically distant Phytophthora species.

    Imano S., Kondou Y., Shibata Y., Kondo T., Tanaka A., Sato I., Chiba S., Kageyama K., Kawakita K., Takemoto D.

    MOLECULAR PLANT-MICROBE INTERACTIONS   32 巻 ( 10 ) 頁: 108-108   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  11. Three novel deoxynivalenol-degrading bacteria isolated from Poaceae planted soils in Japan.

    Morimura H., Chiba S., Takemoto D., Kawakita K., Sato I.

    MOLECULAR PLANT-MICROBE INTERACTIONS   32 巻 ( 10 ) 頁: 25-25   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  12. Nicotiana benthamiana exportin 1 is required for elicitor-induced phytoalexin production, cell death induction, and resistance against potato late blight pathogen Phytophthora infestans 査読有り

    Mizuno Yuri, Imano Sayaka, Camagna Maurizio, Suzuki Takamasa, Tanaka Aiko, Sato Ikuo, Chiba Sotaro, Kawakita Kazuhito, Takemoto Daigo

    JOURNAL OF GENERAL PLANT PATHOLOGY   85 巻 ( 5 ) 頁: 347-355   2019年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s10327-019-00855-9

    Web of Science

  13. Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom (Lentinula edodes) 査読有り

    Lin Yu-Hsin, Fujita Miki, Chiba Sotaro, Hyodo Kiwamu, Andika Ida Bagus, Suzuki Nobuhiro, Kondo Hideki

    VIROLOGY   533 巻   頁: 125 - 136   2019年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.virol.2019.05.008

    Web of Science

  14. Nicotiana benthamiana RanBP1-1 Is Involved in the Induction of Disease Resistance via Regulation of Nuclear-Cytoplasmic Transport of Small GTPase Ran

    Mizuno Yuri, Ohtsu Mina, Shibata Yusuke, Tanaka Aiko, Camagna Maurizio, Ojika Makoto, Mori Hitoshi, Sato Ikuo, Chiba Sotaro, Kawakita Kazuhito, Takemoto Daigo

    FRONTIERS IN PLANT SCIENCE   10 巻   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3389/fpls.2019.00222

    Web of Science

  15. A novel insect-infecting virga/nege-like virus group and its pervasive endogenization into insect genomes

    Kondo Hideki, Chiba Sotaro, Maruyama Kazuyuki, Andika Ida Bagus, Suzuki Nobuhiro

    VIRUS RESEARCH   262 巻   頁: 37-47   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.virusres.2017.11.020

    Web of Science

  16. Nicotiana benthamiana exportin 1 is required for elicitor‑induced phytoalexin production, cell death induction, and resistance against potato late blight pathogen Phytophthora infestans 査読有り 国際誌

    Mizuno Y, Imano S, Camagna M, Suzuki T, Tanaka A, Sato I, Chiba S, Kawakita K, Takemoto D

    J. Gen. Plant Pathol.   85 巻   頁: 347 - 355   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  17. Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom (Lentinula edodes) 査読有り 国際誌

    Lin YH, Fujita M, Chiba S, Hyodo K, Andika IB, Suzuki N, Kondo H

    Virology   533 巻   頁: 125 - 136   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  18. ICTV Virus Taxonomy Profile: Quadriviridae

    Chiba Sotaro, Caston Jose R., Ghabrial Said A., Suzuki Nobuhiro

    JOURNAL OF GENERAL VIROLOGY   99 巻 ( 11 ) 頁: 1480-1481   2018年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1099/jgv.0.001152

    Web of Science

  19. Novel Mitoviruses and a Unique Tymo-Like Virus in Hypovirulent and Virulent Strains of the Fusarium Head Blight Fungus, Fusarium boothii 査読有り 国際共著

    Mizutani Yukiyoshi, Abraham Adane, Uesaka Kazuma, Kondo Hideki, Suga Haruhisa, Suzuki Nobuhiro, Chiba Sotaro

    VIRUSES   10 巻 ( 11 ) 頁: 584   2018年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/v10110584

    Web of Science

  20. Resistance to Phytophthora infestans: exploring genes required for disease resistance in Solanaceae plants

    Takemoto Daigo, Shibata Yusuke, Ojika Makoto, Mizuno Yuri, Imano Sayaka, Ohtsu Mina, Sato Ikuo, Chiba Sotaro, Kawakita Kazuhito, Rin Soriya, Camagna Maurizio

    JOURNAL OF GENERAL PLANT PATHOLOGY   84 巻 ( 5 ) 頁: 312-320   2018年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s10327-018-0801-8

    Web of Science

  21. ICTV Virus Taxonomy Profile: Partitiviridae

    Vainio Eeva J., Chiba Sotaro, Ghabrial Said A., Maiss Edgar, Roossinck Marilyn, Sabanadzovic Sead, Suzuki Nobuhiro, Xie Jiatao, Nibert Max

    JOURNAL OF GENERAL VIROLOGY   99 巻 ( 1 ) 頁: 17-18   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1099/jgv.0.000985

    Web of Science

  22. First Evidence for Internal Ribosomal Entry Sites in Diverse Fungal Virus Genomes

    Chiba Sotaro, Jamal Atif, Suzuki Nobuhiro

    MBIO   9 巻 ( 2 )   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1128/mBio.02350-17

    Web of Science

  23. Rice productivity improvement in Cambodia through the application of technical recommendation in a farmer field school

    Chhay Ngin, Seng Suon, Tanaka Toshiharu, Yamauchi Akira, Cedicol Editha C., Kawakita Kazuhito, Chiba Sotaro

    INTERNATIONAL JOURNAL OF AGRICULTURAL SUSTAINABILITY   15 巻 ( 1 ) 頁: 54-69   2017年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/14735903.2016.1174811

    Web of Science

  24. EIN2-mediated signaling is involved in pre-invasion defense in Nicotiana benthamiana against potato late blight pathogen, Phytophthora infestans

    Rin Soriya, Mizuno Yuri, Shibata Yusuke, Fushimi Mayuka, Katou Shinpei, Sato Ikuo, Chiba Sotaro, Kawakita Kazuhito, Takemoto Daigo

    PLANT SIGNALING & BEHAVIOR   12 巻 ( 4 )   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/15592324.2017.1300733

    Web of Science

  25. Impact of Insecticide Applications on Arthropod Predators and Plant Feeders in Cambodian Rice Fields 査読有り

    Ngin Chhay, Suon Seng, Tanaka Toshiharu, Yamauchi Akira, Kawakita Kazuhito, Chiba Sotaro

    PHYTOBIOMES JOURNAL   1 巻 ( 3 ) 頁: 128-137   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1094/PBIOMES-01-17-0002-R

    Web of Science

  26. Effects of mechanical defoliation and detillering at different growth stages on rice yield in dry season in Cambodia. 査読有り 国際誌

    Ngin C, Suon S, Tanaka T, Yamauchi A, Kawakita K, Chiba S

    Int J Agric Environ Res.   3 巻   頁: 3452 - 3470   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  27. A novel betapartitivirus RnPV6 from Rosellinia necatrix tolerates host RNA silencing but is interfered by its defective RNAs 査読有り 国際誌

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    VIRUS RESEARCH   219 巻   頁: 62 - 72   2016年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The family Partitiviridae comprises of five genera with bi-segmented dsRNA genomes that accommodate members infecting plants, fungi or protists. All partitiviruses with only a few exceptions cause asymptomatic infections. We report the characterization of a novel betapartitivirus termed Rosellinia necatrix partitivirus 6 (RnPV6) from a field isolate of a plant pathogenic fungus, white root rot fungus. RnPV6 has typical partitivirus features: dsRNA1 and dsRNA2 are 2462 and 2499 bps in length encoding RNA dependent RNA polymerase and capsid protein. Purified particles are spherical with a diameter of 30 nm. Taking advantage of infectivity as virions, RnPV6 was introduced into a model filamentous fungal host, chestnut blight fungus to investigate virus/host interactions. Unlike other partitiviruses tested previously, RnPV6 induced profound phenotypic alterations with symptoms characterized by a reduced growth rate and enhanced pigmentation and was tolerant to host RNA silencing. In addition, a variety of defective RNAs derived from dsRNA1 appear after virion transfection. These sub-viral RNAs were shown to interfere with RnPV6 replication, at least for that of cognate segment dsRNA1. Presence of these sub-viral elements resulted in reduced symptom expression by RnPV6, suggesting their nature as defective-interfering RNAs. The features of RnPV6 are similar to but distinct from those of a previously reported alphapartitivirus, Rosellinia necatrix partitivirus 2 that is susceptible to RNA silencing. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.virusres.2015.10.017

    Web of Science

    PubMed

  28. Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi. 査読有り 国際誌

    Kondo H, Hisano S, Chiba S, Maruyama K, Andika IB, Toyoda K, Fujimori F, Suzuki N

    Virus research   219 巻   頁: 39 - 50   2016年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.virusres.2016.05.011

    Web of Science

    PubMed

  29. Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi 査読有り 国際誌

    Hideki Kondo, Sakae Hisano, Sotaro Chiba, Kazuyuki Maruyama, Ida Bagus Andika, Kazuhiro Toyoda, Fumihiro Fujimori, Nobuhiro Suzuki

    VIRUS RESEARCH   213 巻   頁: 353 - 364   2016年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. Powdery mildew fungi are important and widely studied obligate phytopathogenic agents, but there has been no report on mycoviruses infecting these fungi. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least ten totivirus (genus Totivirus)-like sequences, termed red clover powdery mildew-associated totiviruses (RPaTVs). The majority of these sequences shared moderate amino acid sequence identity with each other (<44%) and with other known totiviruses (<59%). Nine of these identified sequences (RPaTV1a, 1b and 2-8) resembled the genome of the prototype totivirus, Saccharomyces cerevisiae virus-L-A (ScV-L-A) in that they contained two overlapping open reading frames (ORFs) encoding a putative coat protein (CP) and an RNA dependent RNA polymerase (RdRp), while one sequence (RPaTV9) showed similarity to another totivirus, Ustilago maydis virus H1 (UmV-H1) that encodes a single polyprotein (CP-RdRp fusion). Similar to yeast totiviruses, each ScV-L-A-like RPaTV contains a-1 ribosomal frameshift site downstream of a predicted pseudoknot structure in the overlapping region of these ORFs, suggesting that the RdRp is translated as a CP-RdRp fusion. Moreover, several ScV-L-A-like sequences were also found by searches of the transcriptome shotgun assembly (TSA) libraries from rust fungi, plants and insects. Phylogenetic analyses show that nine ScV-L-A-like RPaTVs along with ScV-L-A-like sequences derived from TSA libraries are clustered with most established members of the genus Totivirus, while one RPaTV forms a new distinct Glade with UmV-H1, possibly establishing an additional genus in the family. Taken together, our results indicate the presence of diverse, novel totiviruses in the powdery mildew fungus populations infecting red clover plants in the field. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.virusres.2015.11.015

    Web of Science

    PubMed

  30. Genetic diversity of beet necrotic yellow vein virus 査読有り 国際誌

    Tetsuo Tamada, Hideki Kondo, Sotaro Chiba

    Rhizomania     頁: 109 - 131   2016年1月

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    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Springer International Publishing  

    Beet necrotic yellow vein virus (BNYVV) is a member of the genus Benyvirus in the family Benyviridae with multipartite positive-sense single-stranded RNA genomes encapsidated in rigid rod-shaped particles. The members of Benyvirus including four species are similar to those of the family Virgaviridae, in respect of viral particle assembly, movement, and plasmodiophorid transmissibility. Recent studies revealed that ancestors and/or relatives of benyviruses may have infected a wide range of hosts such as plants, insects, algae, and fungi. For phylogenetic analyses of BNYVV genes, worldwide BNYVV isolates form four clades, A-I, A-II, A-III, and B, from which at least ten subgroup isolates (strains) have derived. These original BNYVV types and their progeny strains might have existed in East Asia, and each source had introduced infection to cultivated sugar beet plants and might have spread worldwide only in the last half century. Along with the growth of resistant varieties in rhizomania-infested areas since the 1980s, strong selection pressure has been imposed on the RNA3-encoded p25 gene, and, consequently, resistance-breaking variants that have single amino acid changes in the p25 protein have been generated. RNA5-encoded p26 gene is also associated with resistance breaking as well as symptom severity in sugar beet roots.

    DOI: 10.1007/978-3-319-30678-0_5

    Scopus

  31. Vigorous induction of dicer-like 2 gene by a virus interferes with an unrelated virus in the model phytopathogenic fungus Cryphonectria parasitica. 招待有り 国際誌

    Chiba S, Suzuki N

    PSJ Plant Virus Disease Workshop Report   12 巻   頁: 51 - 57   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  32. Highly activated RNA silencing via strong induction of dicer by one virus can interfere with the replication of an unrelated virus 査読有り 国際誌

    Sotaro Chiba, Nobuhiro Suzuki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   112 巻 ( 35 ) 頁: E4911 - E4918   2015年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Viruses often coinfect single host organisms in nature. Depending on the combination of viruses in such coinfections, the interplay between them may be synergistic, apparently neutral with no effect on each other, or antagonistic. RNA silencing is responsible for many cases of interference or cross-protection between viruses, but such antagonistic interactions are usually restricted to closely related strains of the same viral species. In this study, we present an unprecedented example of RNA silencing-mediated one-way interference between unrelated viruses in a filamentous model fungus, Cryphonectria parasitica. The replication of Rosellinia necatrix victorivirus 1 (RnVV1; Totiviridae) was strongly impaired by coinfection with the prototypic member of the genus Mycoreovirus (MyRV1) or a mutant of the prototype hypovirus (Cryphonectria hypovirus 1, CHV1) lacking the RNA silencing suppressor (CHV1-Delta p69). This interference was associated with marked transcriptional induction of key genes in antiviral RNA silencing, dicer-like 2 (dcl2) and argonaute-like 2 (agl2), following MyRV1 or CHV1-Delta p69 infection. Interestingly, the inhibition of RnVV1 replication was reproduced when the levels of dcl2 and agl2 transcripts were elevated by transgenic expression of a hairpin construct of an endogenous C. parasitica gene. Disruption of dcl2 completely abolished the interference, whereas that of agl2 did not always lead to its abolishment, suggesting more crucial roles of dcl2 in antiviral defense. Taken altogether, these results demonstrated the susceptible nature of RnVV1 to the antiviral silencing in C. parasitica activated by distinct viruses or transgene-derived double-stranded RNAs and provide insight into the potential for broad-spectrum virus control mediated by RNA silencing.

    DOI: 10.1073/pnas.1509151112

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  33. Cymbidium chlorotic mosaic virus, a new sobemovirus isolated from a spring orchid (Cymbidium goeringii) in Japan 査読有り 国際誌

    Hideki Kondo, Shogo Takemoto, Kazuyuki Maruyama, Sotaro Chiba, Ida Bagus Andika, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY   160 巻 ( 8 ) 頁: 2099 - 2104   2015年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER WIEN  

    Cymbidium chlorotic mosaic virus (CyCMV), isolated from a spring orchid (Cymbidium goeringii), was characterized molecularly. CyCMV isometric virions comprise a single, positive-strand RNA genome of 4,083 nucleotides and 30-kDa coat protein. The virus genome contains five overlapping open reading frames with a genomic organization similar to that of sobemoviruses. BLAST searches and phylogenetic analysis revealed that CyCMV is most closely related to papaya lethal yellowing virus, a proposed dicot-infecting sobemovirus (58.8 % nucleotide sequence identity), but has a relatively distant relationship to monocot-infecting sobemoviruses, with only modest sequence identities. This suggests that CyCMV is a new monocot-infecting member of the floating genus Sobemovirus.

    DOI: 10.1007/s00705-015-2460-9

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  34. The chestnut blight fungus for studies on virus/host and virus/virus interactions: From a natural to a model host 査読有り 国際誌

    Ana Eusebio-Cope, Liying Sun, Toru Tanaka, Sotaro Chiba, Shin Kasahara, Nobuhiro Suzuki

    VIROLOGY   477 巻   頁: 164 - 175   2015年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The chestnut blight fungus, Cryphonectria parasitica, is an important plant pathogenic ascomycete. The fungus hosts a wide range of viruses and now has been established as a model filamentous fungus for studying virus/host and virus/virus interactions. This is based on the development of methods for artificial virus introduction and elimination, host genome manipulability, available host genome sequence with annotations, host mutant strains, and molecular tools. Molecular tools include subcellular distribution markers, gene expression reporters, and vectors with regulatable promoters that have been long available for unicellular organisms, cultured cells, individuals of animals and plants, and certain filamentous fungi. A comparison with other filamentous fungi such as Neurospora crassa has been made to establish clear advantages and disadvantages of C parasitica as a virus host. In addition, a few recent studies on RNA silencing vs. viruses in this fungus are introduced. (C) 2014 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.virol.2014.09.024

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  35. Detection and analysis of non-retroviral RNA virus-like elements in plant, fungal, and insect genomes. 査読有り 国際誌

    Hideki Kondo, Sotaro Chiba, Nobuhiro Suzuki

    Methods in molecular biology (Clifton, N.J.)   1236 巻   頁: 73 - 88   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Endogenous non-retroviral RNA like sequences (NRVSs) have been discovered in the genome of a wide range of eukaryotes. These are considered as fossil RNA viral elements integrated into host genomes by as-yet-known mechanisms, and in many cases, those fossils are estimated to be millions-of-years-old. It is likely that the number of NRVS records will increase rapidly due to the growing availability of whole-genome sequences for many kinds of eukaryotes. Discovery of the novel NRVSs and understanding of their phylogenetic relationship with modern viral relatives provide important information on deep evolutionary history of RNA virus-host interactions. In this chapter, therefore, the common strategies for the identification and characterization of endogenous NRVSs from plants, insects, and fungi are described.

    DOI: 10.1007/978-1-4939-1743-3_7

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  36. A novel single-stranded RNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix, with similarity to hypo-like viruses 査読有り 国際誌

    Rui Zhang, Shengxue Liu, Sotaro Chiba, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    FRONTIERS IN MICROBIOLOGY   5 巻   頁: 360   2014年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FRONTIERS RESEARCH FOUNDATION  

    Here we report a biological and molecular characterization of a novel positive-sense RNA virus isolated from a field isolate (NW10) of a filamentous phytopathogenic fungus, the white root rot fungus that is designated as Rosellinia necatrix fusarivirus 1 (RnFV1). A recently developed technology using zinc ions allowed us to transfer RnFV1 to two mycelially incompatible Rosellinia necatrix strains. A biological comparison of the virus-free and -recipient isogenic fungal strains suggested that RnFV1 infects latently and thus has no potential as a virocontrol agent. The virus has an undivided positive-sense RNA genome of 6286 nucleotides excluding a poly (A) tail. The genome possesses two non-overlapping open reading frames (ORFs): a large ORF1 that encodes polypeptides with RNA replication functions and a smaller ORF2 that encodes polypeptides of unknown function. A lack of coat protein genes was suggested by the failure of virus particles from infected mycelia. No evidence was obtained by Northern analysis or classical 5'-RACE for the presence of subgenomic RNA for the downstream ORE Sequence similarities were found in amino-acid sequence between RnFV1 putative proteins and counterparts of a previously reported mycovirus, Fusarium graminearum virus 1 (FgV1). Interestingly, several related sequences were detected by BLAST searches of independent transcriptome assembly databases one of which probably represents an entire virus genome. Phylogenetic analysis based on the conserved RNA-dependent RNA polymerase showed that RnFV1, FgV1, and these similar sequences are grouped in a cluster distinct from distantly related hypoviruses. It is proposed that a new taxonomic family termed Fusariviridae be created to include RnFV1 and FgV1.

    DOI: 10.3389/fmicb.2014.00360

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  37. Biological properties and expression strategy of rosellinia necatrix megabirnavirus 1 analysed in an experimental host, Cryphonectria parasitica 査読有り 国際誌

    Lakha Salaipeth, Sotaro Chiba, Ana Eusebio-Cope, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF GENERAL VIROLOGY   95 巻 ( Pt 3 ) 頁: 740 - 750   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Rosellinia necatrix megabirnavirus 1 (RnMBV1) with a bipartite dsRNA genome (dsRNA1 and dsRNA2) confers hypovirulence to its natural host, the white root rot fungus, and is thus regarded as a potential virocontrol (biocontrol) agent. Each segment has two large ORFs: ORF1 and partially overlapping ORF2 on dsRNA1 encode the major capsid protein (CP) and RNA-dependent RNA polymerase (RdRp), whilst ORF3 and ORF4 on dsRNA2 encode polypeptides with unknown functions. Here, we report the biological and molecular characterization of this virus in the chestnut blight fungus, Cryphonectria parasitica, a filamentous fungus that has been used as a model for mycovirus research. Transfection with purified RnMBV1 particles into an RNA-silencing-defective strain (Delta dcl-2) of C. parasitica and subsequent anastomosis with the WT strain (EP155) resulted in stable persistent infection in both host strains. However, accumulation levels in the two strains were different, being similar to 20-fold higher in Delta dcl-2 than in EP155. Intriguingly, whilst RnMBV1 reduced both virulence and growth rate in Delta dcl-2, it attenuated virulence without affecting significantly other traits in EP155. Western blot analysis using antiserum against recombinant proteins encoded by either ORF1 or ORF2 demonstrated the presence of a 250 kDa protein in purified virion preparations, suggesting that RdRp is expressed as a CP fusion product via a - 1 frameshift. Antiserum against the ORF3-encoded protein allowed the detection of 150, 30 and 23 kDa polypeptides specifically in RnMBV1-infected mycelia. Some properties of an RnMBV1 mutant with genome rearrangements, which occurred after transfection of Delta dcl-2 and EP155, were also presented. This study provides an additional example of C. parasitica serving as a versatile, heterologous fungus for exploring virus host interactions and virus gene expression strategies.

    DOI: 10.1099/vir.0.058164-0

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  38. Transcriptional mapping of the messenger and leader RNAs of orchid fleck virus, a bisegmented negative-strand RNA virus 査読有り 国際誌

    Hideki Kondo, Kazuyuki Maruyama, Sotaro Chiba, Ida Bagus Andika, Nobuhiro Suzuki

    VIROLOGY   452 巻   頁: 166 - 174   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The transcriptional strategy of orchid fleck virus (OFV), which has a two-segmented negative-strand RNA genome and resembles plant nucleorhabdoviruses, remains unexplored. In this study, the transcripts of six genes encoded by OFV RNA1 and RNA2 in the poly(A)-enriched RNA fraction from infected plants were molecularly characterized. All of the OFV mRNAs were initiated at a start sequence 3'-UU-5' with one to three non-viral adenine nucleotides which were added at the 5' end of each mRNA, whereas their 3' termini ended with a 5'-AUUUAAA(U/G)AAAA(A)n-3' sequence. We also identified the presence of polyadenylated short transcripts derived from the 3'-terminal leader regions of both genomic and antigenomic strands, providing the first example of plus- and minus-strand leader RNAs in a segmented minus-strand RNA virus. The similarity in the transcriptional strategy between this bipartite OFV and monopartite rhabdoviruses, especially nucleorhabdoviruses (family Rhabdoviridae) is additional support for their close relationship. (C) 2014 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.virol.2014.01.007

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  39. Complete genome sequence of Habenaria mosaic virus, a new potyvirus infecting a terrestrial orchid (Habenaria radiata) in Japan 査読有り 国際誌

    Hideki Kondo, Takanori Maeda, I. Wayan Gara, Sotaro Chiba, Kazuyuki Maruyama, Tetsuo Tamada, Nobuhiro Suzuki

    ARCHIVES OF VIROLOGY   159 巻 ( 1 ) 頁: 163 - 166   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER WIEN  

    The complete genomic sequence of Habenaria mosaic virus (HaMV), which infects terrestrial orchids (Habenaria radiata), has been determined. The genome is composed of 9,499 nucleotides excluding the 3'-terminal poly(A) tail, encoding a large polyprotein of 3,054 amino acids with the genomic features typical of a potyvirus. Putative proteolytic cleavage sites were identified by sequence comparison to those of known potyviruses. The HaMV polyprotein showed 58 % amino acid sequence identity to that encoded by the most closely related potyvirus, tobacco vein banding mosaic virus. Phylogenetic analysis of the polyprotein amino acid sequence and its coding sequences confirmed that HaMV formed a cluster with the chilli veinal mottle virus group, most of which infect solanaceous plants. These results suggest that HaMV is a distinct member of the genus Potyvirus.

    DOI: 10.1007/s00705-013-1784-6

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  40. [Diverse double-stranded RNA viruses infecting fungi]. 査読有り 国際誌

    Chiba S, Suzuki N

    Uirusu   64 巻 ( 2 ) 頁: 225 - 238   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2222/jsv.64.225

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  41. Characterization of burdock mottle virus, a novel member of the genus Benyvirus, and the identification of benyvirus-related sequences in the plant and insect genomes 査読有り 国際誌

    Hideki Kondo, Shuichi Hirano, Sotaro Chiba, Ida Bagus Andika, Makoto Hirai, Takanori Maeda, Tetsuo Tamada

    Virus Research   177 巻 ( 1 ) 頁: 75 - 86   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The complete nucleotide sequence of the burdock mottle virus (BdMoV) isolated from an edible burdock plant ( Arctium lappa) in Japan has been determined. BdMoV has a bipartite genome, whose organization is similar to RNA1 and RNA2 of benyviruses, beet necrotic yellow vein virus (BNYVV), beet soil-borne mosaic virus (BSBMV), and rice stripe necrosis virus (RSNV). BdMoV RNA1 (7038 nt) contains a single open reading frame (ORF) encoding a 249-kDa polypeptide that consists of methyl-transferase, helicase, papain-like protease, AlkB-like, and RNA-dependent RNA polymerase domains. The AlkB-like domain sequence is not present in the proteins encoded by other known benyviruses, but is found in replication-associated proteins of viruses mainly belonging to the families Alfaflexiviridae and Betaflexiviridae. BdMoV RNA2 (4315 nt) contains six ORFs that are similar to those of benyviruses: these are coat protein (CP), CP readthrough, triple gene block movement and cysteine-rich proteins. Phylogenetic analyses showed that BdMoV is more closely related to BNYVV and BSBMV than to RSNV. Database searches showed that benyvirus replicase-related sequences are present in the chromosomes of a chickpea plant ( Cicer arietinum) and a blood-sucking insect ( Rhodnius prolixus). Some other benyvirus-related sequences are found in the transcriptome shotgun libraries of a few species of plants and a bark beetle. Our results show that BdMoV is a distinct species of the genus Benyvirus and that ancestral and extant benyviruses may have infected or currently infect a wide range of hosts, including plants and insects. © 2013 Elsevier B.V.

    DOI: 10.1016/j.virusres.2013.07.015

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  42. Orchid fleck virus structural proteins N and P form intranuclear viroplasm like structures in the absence of viral infection 査読有り 国際誌

    Hideki Kondo, Sotaro Chiba, Ida Bagus Andika, Kazuyuki Maruyama, Tetsuo Tamada, Nobuhiro Suzuki

    Journal of Virology   87 巻 ( 13 ) 頁: 7423 - 7434   2013年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Orchid fleck virus (OFV) has a unique two-segmented negative-sense RNA genome that resembles that of plant nucleorhabdoviruses. In infected plant cells, OFV and nucleorhabdoviruses induce an intranuclear electron-lucent viroplasm that is believed to be the site for virus replication. In this study, we investigated the molecular mechanism by which OFV viroplasms are produced in vivo. Among OFV-encoded proteins, the nucleocapsid protein (N) and the putative phosphoprotein (P) were present in nuclear fractions of OFV-infected Nicotiana benthamiana plants. Transient coexpression of N and P, in the absence of virus infection, was shown to be sufficient for formation of an intranuclear viroplasm-like structure in plant cells. When expressed independently as a fluorescent protein fusion product in uninfected plant cells, N protein accumulated throughout the cell, while P protein accumulated in the nucleus. However, the N protein, when coexpressed with P, was recruited to a subnuclear region to induce a large viroplasm-like focus. Deletion and substitution mutagenesis demonstrated that the P protein contains a nuclear localization signal (NLS). Artificial nuclear targeting of the N-protein mutant was insufficient for formation of viroplasm-like structures in the absence of P. A bimolecular fluorescence complementation assay confirmed interactions between the N and P proteins within subnuclear viroplasm-like foci and interactions of two of the N. benthamiana importin-α homologues with the P protein but not with the N protein. Taken together, our results suggest that viroplasm formation by OFV requires nuclear accumulation of both the N and P proteins, which is mediated by P-NLS, unlike nucleorhabdovirus viroplasm utilizing the NLS on protein N. © 2013, American Society for Microbiology.

    DOI: 10.1128/JVI.00270-13

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  43. A Novel Victorivirus from a Phytopathogenic Fungus, Rosellinia necatrix, Is Infectious as Particles and Targeted by RNA Silencing 査読有り 国際誌

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   87 巻 ( 12 ) 頁: 6727 - 6738   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    A novel victorivirus, termed Rosellinia necatrix victorivirus 1 (RnVV1), was isolated from a plant pathogenic ascomycete, white root rot fungus Rosellinia necatrix, coinfected with a partitivirus. The virus was molecularly and biologically characterized using the natural and experimental hosts (chestnut blight fungus, Cryphonectria parasitica). RnVV1 was shown to have typical molecular victorivirus attributes, including a monopartite double-stranded RNA genome with two open reading frames (ORFs) encoding capsid protein (CP) and RNA-dependent RNA polymerase (RdRp), a UAAUG pentamer presumed to facilitate the coupled termination/reinitiation for translation of the two ORFs, a spherical particle structure similar to 40 nm in diameter, and moderate levels of CP and RdRp sequence identity (34 to 58%) to those of members of the genus Victorivirus within the family Totiviridae. A reproducible transfection system with purified RnVV1 virions was developed for the two distinct fungal hosts. Transfection assay with purified RnVV1 virions combined with virus elimination by hyphal tipping showed that the effects of RnVV1 on the phenotype of the natural host were negligible. Interestingly, comparison of the RNA silencing-competent (standard strain EP155) and -defective (Delta dcl-2) strains of C. parasitica infected with RnVV1 showed that RNA silencing acted against the virus to repress its replication, which was restored by coinfection with hypovirus or transgenic expression of an RNA silencing suppressor, hypovirus p29. Phenotypic changes were observed in the Delta dcl-2 strain but not in EP155. This is the first reported study on the host range expansion of a Totiviridae member that is targeted by RNA silencing.

    DOI: 10.1128/JVI.00557-13

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  44. Effects of Defective Interfering RNA on Symptom Induction by, and Replication of, a Novel Partitivirus from a Phytopathogenic Fungus, Rosellinia necatrix 査読有り 国際誌

    Sotaro Chiba, Yu-Hsin Lin, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   87 巻 ( 4 ) 頁: 2330 - 2341   2013年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    A novel mycovirus termed Rosellinia necatrix partitivirus 2 (RnPV2), isolated from a phytopathogenic fungus, Rosellinina necatrix strain W57, was molecularly and biologically characterized in both natural and experimental host fungi. Three double-stranded RNA (dsRNA) segments, dsRNA1, dsRNA2, and defective interfering dsRNA1 (DI-dsRNA1), whose sizes were approximately 2.0, 1.8, and 1.7 kbp, respectively, were detected in W57. While the dsRNA2 sequence, encoding the coat protein, was reported previously, dsRNA1 and DI-dsRNA1 were shown to encode competent and defective (truncated) RNA-dependent RNA polymerase, respectively. Artificial introduction of RnPV2 into an RNA silencing-defective, Dicer-like 2 knockout mutant (Delta dcl-2) of a nonnatural host, Cryphonectria parasitica (chestnut blight fungus), resulted in successful infection by the DI-dsRNA1-carrying and -free RnPV2. The DI-dsRNA1-free RnPV2 strain was characterized by a higher ratio of accumulation of the intact dsRNA1 to dsRNA2, enhanced replication and severer symptom expression, compared with the DI-carrying strain. These findings confirmed the nature of DI-dsRNA1 as a DI-RNA. Both viral strains replicated to higher levels in a Delta dcl-2 mutant than in a wild-type C. parasitica fungal strain (EP155) and induced severe symptoms in the Delta dcl-2 mutant but subtle symptoms in EP155, indicating that the host RNA silencing targets the partitivirus. No obvious phenotypic effects of infection by either virus strain were detected in the natural host fungus. These combined results represent the first example of a partitivirus with DI-RNA that alters viral symptom induction in a host-dependent manner.

    DOI: 10.1128/JVI.02835-12

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  45. The Benyvirus RNA Silencing Suppressor Is Essential for Long-Distance Movement, Requires Both Zinc-Finger and NoLS Basic Residues but Not a Nucleolar Localization for Its Silencing-Suppression Activity 査読有り 国際誌

    Sotaro Chiba, Kamal Hleibieh, Alice Delbianco, Elodie Klein, Claudio Ratti, Veronique Ziegler-Graff, Salah Bouzoubaa, David Gilmer

    MOLECULAR PLANT-MICROBE INTERACTIONS   26 巻 ( 2 ) 頁: 168 - 181   2013年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYTOPATHOLOGICAL SOC  

    The RNA silencing-suppression properties of Beet necrotic yellow vein virus (BNYVV) and Beet soil-borne mosaic virus (BSBMV) cysteine-rich p14 proteins have been investigated. Suppression of RNA silencing activities were made evident using viral infection of silenced Nicotiana benthamiana 16C, N. benthamiana agroinfiltrated with green fluorescent protein (GFP), and GF-FG hairpin triggers supplemented with viral suppressor of RNA silencing (VSR) constructs or using complementation of a silencing-suppressor-defective BNYVV virus in Chenopodium quinoa. Northern blot analyses of small-interfering RNAs (siRNAs) in agroinfiltration tests revealed reduced amounts of siRNA, especially secondary siRNA, suggesting that benyvirus VSR act downstream of the siRNA production. Using confocal laser-scanning microscopy imaging of infected protoplasts expressing functional p14 protein fused to an enhanced GFP reporter, we showed that benyvirus p14 accumulated in the nucleolus and the cytoplasm independently of other viral factors. Site-directed mutagenesis showed the importance of the nucleolar localization signal embedded in a C4 zinc-finger domain in the VSR function and intrinsic stability of the p14 protein. Conversely, RNA silencing suppression appeared independent of the nucleolar localization of the protein, and a correlation between BNYVV VSR expression and long-distance movement was established.

    DOI: 10.1094/MPMI-06-12-0142-R

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  46. Evidence for negative-strand RNA virus infection in fungi 査読有り 国際誌

    Hideki Kondo, Sotaro Chiba, Kazuhiro Toyoda, Nobuhiro Suzuki

    Virology   435 巻 ( 2 ) 頁: 201 - 209   2013年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Fungal viruses comprise two groups: a major group of five families with double-stranded RNA genomes and a minor group with positive-sense single-stranded (ss)RNA genomes. Although many fungal viruses have been identified, no negative-stranded (-)ssRNA mycoviruses have been reported. Here we present two lines of evidence suggesting the presence of (-)ssRNA viruses in filamentous fungi based on an exhaustive search using extant (-)ssRNA viruses as queries. This revealed (-)ssRNA virus L protein-like sequences in the genome of a phytopathogenic obligate ascomycete, Erysiphe pisi. A similar search for (-)ssRNA viruses in fungal transcriptome shotgun assembly libraries demonstrated that two independent libraries from Sclerotinia homoeocarpa, another phytopathogenic ascomycete, contained several sequences considered to correspond to the entire mononegavirus L gene and likely originating from an infecting (-)ssRNA virus. These results provide strong evidence for both ancient and extant (-)ssRNA virus infections in fungi. © 2012 Elsevier Inc.

    DOI: 10.1016/j.virol.2012.10.002

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  47. A novel quadripartite dsRNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix 査読有り 国際誌

    Yu-Hsin Lin, Sotaro Chiba, Akio Tani, Hideki Kondo, Atsuko Sasaki, Satoko Kanematsu, Nobuhiro Suzuki

    VIROLOGY   426 巻 ( 1 ) 頁: 42 - 50   2012年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Here we report the biological and molecular attributes of a novel dsRNA virus isolated from Rosellinia necatrix, a filamentous phytopathogenic fungus. The virus, termed Rosellinia necatrix quadrivirus 1 (RnQV1), forms rigid spherical particles approximately 45 nm in diameter in infected mycelia. The particles contain 4 dsRNA segments, dsRNA1 to dsRNA4, with a size range of 4.9 to 3.7 kbp, each possessing a single large ORF. A comparison of the virus-infected and -cured isogenic fungal strains suggested that RnQV1 infection has no appreciable phenotypic effects. Phylogenetic analysis using the dsRNA3-encoded RdRp sequence revealed that RnQV1 is more distantly related to quadripartite chrysoviruses than to monopartite totiviruses, and is placed in a distinct group from other mycoviruses. No significant sequence similarities were evident between known proteins and RnQV1 structural proteins shown to be encoded by dsRNA2 or dsRNA4. These suggest that RnQV1 is a novel latent virus, belonging to a new family. (C) 2012 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.virol.2012.01.013

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  48. Widespread Endogenization of Genome Sequences of Non-Retroviral RNA Viruses into Plant Genomes 査読有り 国際誌

    Sotaro Chiba, Hideki Kondo, Akio Tani, Daisuke Saisho, Wataru Sakamoto, Satoko Kanematsu, Nobuhiro Suzuki

    PLOS PATHOGENS   7 巻 ( 7 )   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Non-retroviral RNA virus sequences (NRVSs) have been found in the chromosomes of vertebrates and fungi, but not plants. Here we report similarly endogenized NRVSs derived from plus-, negative-, and double-stranded RNA viruses in plant chromosomes. These sequences were found by searching public genomic sequence databases, and, importantly, most NRVSs were subsequently detected by direct molecular analyses of plant DNAs. The most widespread NRVSs were related to the coat protein (CP) genes of the family Partitiviridae which have bisegmented dsRNA genomes, and included plant-and fungus-infecting members. The CP of a novel fungal virus (Rosellinia necatrix partitivirus 2, RnPV2) had the greatest sequence similarity to Arabidopsis thaliana ILR2, which is thought to regulate the activities of the phytohormone auxin, indole-3-acetic acid (IAA). Furthermore, partitivirus CP-like sequences much more closely related to plant partitiviruses than to RnPV2 were identified in a wide range of plant species. In addition, the nucleocapsid protein genes of cytorhabdoviruses and varicosaviruses were found in species of over 9 plant families, including Brassicaceae and Solanaceae. A replicase-like sequence of a betaflexivirus was identified in the cucumber genome. The pattern of occurrence of NRVSs and the phylogenetic analyses of NRVSs and related viruses indicate that multiple independent integrations into many plant lineages may have occurred. For example, one of the NRVSs was retained in Ar. thaliana but not in Ar. lyrata or other related Camelina species, whereas another NRVS displayed the reverse pattern. Our study has shown that single-and double-stranded RNA viral sequences are widespread in plant genomes, and shows the potential of genome integrated NRVSs to contribute to resolve unclear phylogenetic relationships of plant species.

    DOI: 10.1371/journal.ppat.1002146

    Web of Science

  49. The Evolutionary History of Beet necrotic yellow vein virus Deduced from Genetic Variation, Geographical Origin and Spread, and the Breaking of Host Resistance 査読有り 国際誌

    Soutaro Chiba, Hideki Kondo, Masaki Miyanishi, Ida Bagus Andika, Chenggui Han, Tetsuo Tamada

    MOLECULAR PLANT-MICROBE INTERACTIONS   24 巻 ( 2 ) 頁: 207 - 218   2011年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYTOPATHOLOGICAL SOC  

    Beet necrotic yellow vein virus (BNYVV) is an economically important pathogen of sugar beet and has been found worldwide, probably as the result of recent worldwide spread. The BNYVV genome consists of four or five RNA components. Here, we report analysis of sequence variation in the RNA3-p25, RNA4-p31, RNA2-CP, and RNA5-p26 genes of 73 worldwide isolates. The RNA3-p25 gene encodes virulence and avirulence factors. These four sets of gene sequences each fell into two to four groups, of which the three groups of p25 formed eight subgroups with different geographical distributions. Each of these subgroup isolates (strains) could have arisen from four original BNYVV population and their mixed infections. The genetic diversity for BNYVV was relatively small. Selection pressure varied greatly depending on the BNYVV gene and geographical location. Isolates of the Italy strain, in which p25 was subject to the strongest positive selection, were able to overcome the Rz1-host resistance gene to differing degrees, whereas other geographically limited strains could not. Resistance-breaking variants were generated by p25 amino acid changes at positions 67 and 68. Our studies suggest that BNYVV originally evolved in East Asia and has recently become a pathogen of cultivated sugar beet followed by the emergence of new resistance-breaking variants.

    DOI: 10.1094/MPMI-10-10-0241

    Web of Science

  50. [Mycoviruses and virocontrol]. 査読有り 国際誌

    Chiba S, Kondo H, Kanematsu S, Suzuki N

    Uirusu   60 巻 ( 2 ) 頁: 163 - 176   2010年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2222/jsv.60.163

    PubMed

  51. A Novel Bipartite Double-Stranded RNA Mycovirus from the White Root Rot Fungus Rosellinia necatrix: Molecular and Biological Characterization, Taxonomic Considerations, and Potential for Biological Control 査読有り 国際誌

    Sotaro Chiba, Lakha Salaipeth, Yu-Hsin Lin, Atsuko Sasaki, Satoko Kanematsu, Nobuhiro Suzuki

    JOURNAL OF VIROLOGY   83 巻 ( 24 ) 頁: 12801 - 12812   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    White root rot, caused by the ascomycete Rosellinia necatrix, is a devastating disease worldwide, particularly in fruit trees in Japan. Here we report on the biological and molecular properties of a novel bipartite double-stranded RNA (dsRNA) virus encompassing dsRNA-1 (8,931 bp) and dsRNA-2 (7,180 bp), which was isolated from a field strain of R. necatrix, W779. Besides the strictly conserved 5' (24 nt) and 3' (8 nt) terminal sequences, both segments show high levels of sequence similarity in the long 5' untranslated region of approximately 1.6 kbp. dsRNA-1 and -2 each possess two open reading frames (ORFs) named ORF1 to -4. Although the protein encoded by 3'-proximal ORF2 on dsRNA-1 shows sequence identities of 22 to 32% with RNA-dependent RNA polymerases from members of the families Totiviridae and Chrysoviridae, the remaining three virus-encoded proteins lack sequence similarities with any reported mycovirus proteins. Phylogenetic analysis showed that the W779 virus belongs to a separate clade distinct from those of other known mycoviruses. Purified virions similar to 50 nm in diameter consisted of dsRNA-1 and -2 and a single major capsid protein of 135 kDa, which was shown by peptide mass fingerprinting to be encoded by dsRNA-1 ORF1. We developed a transfection protocol using purified virions to show that the virus was responsible for reduction of virulence and mycelial growth in several host strains. These combined results indicate that the W779 virus is a novel bipartite dsRNA virus with potential for biological control (virocontrol), named Rosellinia necatrix megabirna-virus 1 (RnMBV1), that possibly belongs to a new virus family.

    DOI: 10.1128/JVI.01830-09

    Web of Science

    PubMed

  52. Coupled termination/reinitiation for translation of the downstream open reading frame B of the prototypic hypovirus CHV1-EP713 査読有り 国際誌

    Li-hua Guo, Liying Sun, Sotaro Chiba, Hiroyuki Araki, Nobuhiro Suzuki

    NUCLEIC ACIDS RESEARCH   37 巻 ( 11 ) 頁: 3645 - 3659   2009年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Cryphonectria hypovirus 1 (CHV1), associated with the picorna-like superfamily, infects the chestnut blight fungus and attenuates the virulence of the host fungus. The genomic RNA of the virus has two continuous open reading frames, A and B, separated by the pentanucleotide UAAUG. We present here evidence suggesting that ORF B is translated from genome-sized virus mRNA by a coupled termination/reinitiation mechanism mediated by the pentamer. In the coupled translation, the overlapping UAA and AUG triplets serve as the stop codon of ORF A and the initiator of ORF B, respectively. This was established by the use of a luciferase assay with a basic construct containing the ORF A sequence and the firefly luciferase gene while retaining the pentamer between the two coding sequences. The proportion of ribosomes reinitiating translation after terminating was determined to be 2.54.4 by three independent assay systems in fungal and insect cells. Use of a series of mutant constructs identified two sequence elements, the pentamer and the p40 sequence, that affect the efficiency of coupled translation and virus replication. Together, these results provide the first example of coupled translation facilitated by the pentanucleotide UAAUG in the kingdom Fungi. The mechanism by which the preceding p40-coding sequence promotes reinitiation is discussed.

    DOI: 10.1093/nar/gkp224

    Web of Science

    PubMed

  53. Identification of amino acids of the beet necrotic yellow vein virus p25 protein required for induction of the resistance response in leaves of Beta vulgaris plants 査読有り 国際誌

    Soutaro Chiba, Masaki Miyanishi, Ida Bagus Andika, Hideki Kondo, Tetsuo Tamada

    JOURNAL OF GENERAL VIROLOGY   89 巻   頁: 1314 - 1323   2008年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MICROBIOLOGY SOC  

    The RNA3-encoded p25 protein of beet necrotic yellow vein virus (BNYVV) is responsible for the production of rhizomania symptoms of sugar beet roots (Beta vulgaris subsp. vulgaris). Here, it was found that the presence of the p25 protein is also associated with the resistance response in rub-inoculated leaves of sugar beet and wild beet (Beta vulgaris subsp. maritima) plants. The resistance phenotype displayed a range of symptoms from no visible lesions to necrotic or greyish lesions at the inoculation site, and only very low levels of virus and viral RNA accumulated. The susceptible phenotype showed large, bright yellow lesions and developed high levels of virus accumulation. In roots after Polymyxa betae vector inoculation, however, no drastic differences in virus and viral RNA accumulation levels were found between plants with susceptible and resistant phenotypes, except at an early stage of infection. There was a genotype-specific interaction between BNYVV strains and two selected wild beet lines (MR1 and MR2) and sugar beet cultivars. Sequence analysis of natural BNYVV isolates and site-directed mutagenesis of the p25 protein revealed that 3 aa residues at positions 68, 70 and 179 are important in determining the resistance phenotype, and that host-genotype specificity is controlled by single amino acid changes at position 68. The mechanism of the occurrence of resistance-breaking BNYVV strains is discussed.

    DOI: 10.1099/vir.0.83624-0

    Web of Science

▼全件表示

書籍等出版物 2

  1. Encyclopedia of Virology, 4th Edition, vol. 4,

    Chiba S.( 担当: 単著 ,  範囲: Fusariviruses (Unassigned))

    Oxford: Academic Press.  2020年1月 

     詳細を見る

    担当ページ:557-581   記述言語:英語 著書種別:事典・辞書

    DOI: 10.1016/B978-0-12-809633-8.21276-4

  2. Rhizomania

    Tamada T, Kondo H, Chiba S( 担当: 共著 ,  範囲: Genetic diversity of beet necrotic yellow vein virus (Chapter 5))

    Springer, Cham  2016年9月 

     詳細を見る

    担当ページ:109-133   記述言語:英語 著書種別:学術書

    DOI: 10.1007/978-3-319-30678-0_5

MISC 5

  1. 宿主ゲノム上に存在する RNA ウイルス感染記録を紐解く. 招待有り 国際誌

    近藤秀樹, 千葉壮太郎, 鈴木信弘  

    植物感染生理談話会論文集50 巻   頁: 133 - 142   2015年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  2. Discovery of negative-strand RNA viruses in fungi. 国際誌

    Kondo H, Chiba S, Suzuki N  

    Proceedings of the 9th Sympodium of International Working Group on Plant Viruses with Fungal Vectors   頁: 55 - 57   2014年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)  

  3. Mutational analysis of BNYVV p25 protein for symptom induction in systemic host Beta macrocarpa. 国際誌

    Chiba S, Kondo H, Suzuki N, Tamada T  

    Proceedings of the 9th Sympodium of International Working Group on Plant Viruses with Fungal Vectors   頁: 15 - 18   2014年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)  

  4. Viral and host factors involved in symptom induction and replication of RNA viruses infecting phytopathogenic fungi. 国際誌

    Salaipeth, L, Lin, Y.-H, Eusebio-Cope, A, Tanaka, T, Chiba, S, Suzuki, N  

    Proceeding of the International Symposium on Fungal Genetics and Genomics   頁: 92 - 94   2009年

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(国際会議プロシーディングズ)  

  5. The p25 protein of beet necrotic yellow vein virus has a dual role as avirulence and avirulence determinant in leaves of Beta vurgalis plants. 国際誌

    Tamada T, Chiba S, Andika IB, Kondo H  

    Proceedings of the 7th Sympodium of International Working Group on Plant Viruses with Fungal Vectors   頁: 13 - 16   2008年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)  

講演・口頭発表等 1

  1. テンサイそう根病ウイルス(BNYVV)「成り立ち、病原性、抵抗性、現在と将来」~ウイルス学の視点から~ 招待有り

    千葉壮太郎

    テンサイそう根病研修会  2020年10月1日 

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    開催年月日: 2020年10月1日

    記述言語:日本語   会議種別:公開講演,セミナー,チュートリアル,講習,講義等  

    開催地:北海道常呂郡、訓子府町公民館  

共同研究・競争的資金等の研究課題 4

  1. ヴァイロコントロール因子(菌類ウイルス)の宿主糸状菌感染機構 国際共著

    2018年04月 - 2021年03月

    全国共同利用共同研究事業(岡山大IPSR) 

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    担当区分:研究代表者  資金種別:競争的資金

  2. 植物炭疽病菌を制御するマイコウイルスの探索

    2017年04月 - 2020年03月

    国際交流事業(二国間交流事業 共同研究 アジア) 

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

  3. フザリウム属菌のヴァイロコントロールを目指した菌類ウイルスの探索と同定 国際共著

    2015年04月 - 2018年03月

    全国共同利用共同研究事業(岡山大IPSR) 

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

  4. 菌類ウイルスIRESの探索と応用展開 国際共著

    2015年04月 - 2017年03月

    豊秋奨学会研究費助成事業 

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    資金種別:競争的資金

科研費 5

  1. 菌類ウイルスによる焼酎生産菌の一次・二次代謝産物生産機構に関する研究

    研究課題/研究課題番号:20K05791  2020年04月 - 2023年03月

    日本学術振興会  研究費助成事業 基盤研究(C) 

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    担当区分:研究分担者  資金種別:競争的資金

  2. 東南アジアにおけるイネオレンジ葉ファイトプラズマ病の発生拡大メカニズム 国際共著

    研究課題/研究課題番号:18KK0171  2018年10月 - 2022年03月

    日本学術振興会  国際共同研究加速基金(国際共同研究強化(B)) 

    千葉壮太郎

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

  3. 寄主特異性を決定する共生ウイルスおよびウイルス様粒子の類似性および相違性の解明

    研究課題/研究課題番号:17H03942  2017年04月 - 2021年03月

    日本学術振興会  研究費助成事業 基盤研究(B) 

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    担当区分:研究分担者  資金種別:競争的資金

  4. 卵菌PAMPエリシターに対する植物の抵抗性誘導機構の解明 国際共著

    研究課題/研究課題番号:17H03963  2017年04月 - 2020年03月

    日本学術振興会  研究費助成事業  基盤研究(B) 

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    資金種別:競争的資金

  5. 菌類RNAウイルスの新規リボゾーム内部進入部位の機能構造 国際共著

    研究課題/研究課題番号:15H06276  2015年08月 - 2017年03月

    日本学術振興会  科学研究費補助金 

    千葉壮太郎

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

 

担当経験のある科目 (本学) 7

  1. バイオテクノロジー

    2020

  2. 資源生物科学基盤実験実習(分担)

    2020

  3. 海外実地研修(分担)

    2020

  4. 海外学生受入研修(分担)

    2020

  5. 植物病理学特論

    2020

  6. 資源生物科学実験実習1、2(分担)

    2020

  7. バイオテクノロジー

    2019

▼全件表示

担当経験のある科目 (本学以外) 1

  1. 農業環境微生物学(非常勤・分担)

    2016年4月 - 現在 名城大学)

 

社会貢献活動 1

  1. 植物ウイルス病の発生動向分析

    助言・指導

    2018年4月 - 現在

学術貢献活動 1

  1. アジア諸国の国家中枢人材養成プログラム

    名古屋大学アジアサテライトキャンパス学院  2015年1月1日 - 現在