Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

Award type：Honored in official journal of a scientific society, scientific journal  Country：Japan

Country：Japan

Country：Japan

Country：Japan

Country：Japan

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Biotechnology Reports  

Three-dimensional cell spheroids are superior cell-administration form for cell-based therapy which generally exhibit superior functionality and long-term survival after transplantation. Here, we nondestructively measured the oxygen consumption rate of cell spheroids using an on-chip electrochemical device (OECD) and examined whether this rate can be used as a marker to estimate the quality of cell spheroids. Cell spheroids containing NanoLuc luciferase-expressing mouse mesenchymal stem cell line C3H10T1/2 (C3H10T1/2/Nluc) were prepared. Spheroids of high or low quality were prepared by altering the medium change frequency. After transplantation into mice, the high-quality C3H10T1/2/Nluc spheroids exhibited a higher survival rate than the low-quality ones. The oxygen consumption rate of the high-quality C3H10T1/2/Nluc spheroids was maintained at high levels, whereas that of the low-quality spheroids decreased with time. These results indicate that OECD-based measurement of the oxygen consumption rate can be used to estimate the quality of cell spheroids without destructive analysis of the spheroids.

DOI： 10.1016/j.btre.2022.e00766

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Cells  

In vitro neuromuscular junction (NMJ) models are powerful tools for studying neuromuscular disorders. Although linearly patterned culture surfaces have been reported to be useful for the formation of in vitro NMJ models using mouse motor neuron (MNs) and skeletal muscle (SkM) myotubes, it is unclear how the linearly patterned culture surface increases acetylcholine receptor (AChR) clustering, one of the steps in the process of NMJ formation, and whether this increases the in vitro NMJ formation efficiency of co-cultured human MNs and SkM myotubes. In this study, we investigated the effects of a linearly patterned culture surface on AChR clustering in myotubes and examined the possible mechanism of the increase in AChR clustering using gene expression analysis, as well as the effects of the patterned surface on the efficiency of NMJ formation between co-cultured human SkM myotubes and human iPSC-derived MNs. Our results suggest that better differentiation of myotubes on the patterned surface, compared to the flat surface, induced gene expression of integrin α7 and AChR ε-subunit, thereby increasing AChR clustering. Furthermore, we found that the number of NMJs between human SkM cells and MNs increased upon co-culture on the linearly patterned surface, suggesting the usefulness of the patterned surface for creating in vitro human NMJ models.

DOI： 10.3390/cells11233760

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Journal of Plant Research  

Plasmodesmata are unique channel structures in plants that link the fluid cytoplasm between adjacent cells. Plants have evolved these microchannels to allow trafficking of nutritious substances as well as regulatory factors for intercellular communication. However, tracking the behavior of plasmodesmata in real time is difficult because they are located inside tissues. Hence, a system was constructed to monitor the movement of substances by plasmodesmata using tobacco BY-2 cells, which are linearly organized cells, and a microfluidic device that traps them in place and facilitates observation. After targeting one cell for photobleaching, recovery of the lost H2B-GFP protein was detected within 200 min. No recovery was detected in that time frame by photobleaching the entire cell filaments. This suggested that the recovery of H2B-GFP protein was not due to de novo protein synthesis, but rather to translocation from neighboring cells. The transport of H2B-GFP protein was not observed when sodium chloride, a compound known to cause plasmodesmata closure, was present in the microfluid channel. Thus, using the microfluidic device and BY-2 cells, it was confirmed that the behavior of plasmodesmata could be observed in real time under controllable conditions.

DOI： 10.1007/s10265-022-01406-8

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Biotechnology and Bioengineering  

Skeletal muscle atrophy is characterized by decreases in protein content, myofiber diameter, and contractile force generation. As muscle atrophy worsens the quality of life, the development of anti-atrophic substances is desirable. In this study, we aimed to demonstrate a screening process for anti-atrophic peptides using photo-cleavable peptide array technology and human contractile atrophic muscle models. We developed a 96-well system and established a screening process with less variability. Dexamethasone-induced human atrophic tissue was constructed in the system. Eight peptides were selected from the literature and used for the screening of peptides for preventing the decrease of the contractile forces of tissues. The peptide QIGFIW, which showed preventive activity, was selected as the seed sequence. As a result of amino acid substitution, we obtained QIGFIQ as a peptide with higher anti-atrophic activity. These results indicate that the combinatorial use of the photo-cleavable peptide array technology and 96-well screening system could comprise a powerful approach to obtaining anti-atrophic peptides, and suggest that the 96-well screening system and atrophic model represent a practical and powerful tool for the development of drugs/functional food ingredients.

DOI： 10.1002/bit.28125

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Language：English   Publishing type：Research paper (scientific journal)  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Journal of Bioscience and Bioengineering  

Heat-treated porous silica gel (HT silica gel) previously developed by our group has selectively adsorbed cationic peptides at a pH of 7. Therefore, we focused on the use of antimicrobial peptides (AMPs) as bioactive peptides (BPs). First, 32 AMPs and 32 randomly designed peptides were generated using Fmoc solid synthesis, and their adsorption ratio to HT-silica gel was investigated. Thirty two AMPs showed a relatively higher adsorption ratio of 58.8% compared to that of randomly designed peptides, which was 35.3%. Desorption conditions were investigated using Amyl-1-18 antimicrobial peptides. Next, pepsin hydrolysate from rice endosperm protein (REP) powder was prepared by ourselves. The REP hydrolysate containing dry matter (7.5 mg) was applied to the adsorption/desorption (AD) procedure using HT silica gel to obtain 1.6 mg of AD hydrolysate. When the two hydrolysates were subjected to mass spectrometry, 305 concentrated peptides were obtained. In total, 26 peptides with high content and high enrichment ratios were listed and synthesized. When the antimicrobial activity of these 26 peptides was evaluated using Cutibacterium acnes, five peptides consisting of 12–27 amino acids were identified as novel AMPs. Two of these peptides, which were derived from rice glutelin, showed antimicrobial activity against all four microbes, including Porphyromonas gingivalis, Escherichia coli, and Streptococcus mutans. In the present study, we showed that AMPs could be easily enriched from protein hydrolysate using HT silica gel. The adsorption/desorption procedure using HT silica gel was confirmed to be a useful tool for convenient BP separation.

DOI： 10.1016/j.jbiosc.2021.11.002

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Biotechnology Journal  

Background: Because of the excellent therapeutic potential, mesenchymal stem cells (MSCs) have been used as cell therapeutics for various diseases. However, the survival rate and duration of MSCs after transplantation are extremely low and short, respectively. To solve these problems, in this study, we prepared multicellular spheroids of MSCs and investigated their survival and function after intravenous injection in mice. Methods and Results: The murine adipose-derived MSC line m17.ASC was cultured in agarose-based microwell plates to obtain size-controlled m17.ASC spheroids of an average diameter and cell number of approximately 170 μm and 1100 cells/spheroid, respectively. The intravenously injected m17.ASC spheroids mainly accumulated in the lung and showed a higher survival rate than suspended m17.ASC cells during the experimental period of 7 days. m17.ASC spheroids efficiently reduced the lipopolysaccharide-induced increase in plasma concentrations of interleukin-6 and tumor necrosis factor-α. Conclusions: These results indicate that spheroid formation improved the pulmonary delivery and survival of MSCs, as well as their therapeutic potential against inflammatory pulmonary diseases.

DOI： 10.1002/biot.202100137

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：PLoS ONE  

The tobacco BY-2 cell line has been used widely as a model system in plant cell biology. BY-2 cells are nearly transparent, which facilitates cell imaging using fluorescent markers. As cultured cells are drifted in the medium, therefore, it was difficult to observe them for a long period. Hence, we developed a microfluidic device that traps BY-2 cells and fixes their positions to allow monitoring the physiological activity of cells. The device contains 112 trap zones, with parallel slots connected in series at three levels in the flow channel. BY-2 cells were cultured for 7 days and filtered using a sieve and a cell strainer before use to isolate short cell filaments consisting of only a few cells. The isolated cells were introduced into the flow channel, resulting in entrapment of cell filaments at 25 out of 112 trap zones (22.3%). The cell numbers increased through cell division from 1 to 4 days after trapping with a peak of mitotic index on day 2. Recovery experiments of fluorescent proteins after photobleaching confirmed cell survival and permeability of plasmodesmata. Thus, this microfluidic device and one-dimensional plant cell samples allowed us to observe cell activity in real time under controllable conditions.

DOI： 10.1371/journal.pone.0266982

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Seibutsu-kogaku Kaishi  

For the prediction of protease digestion site, 1990 kinds of tetramer peptide were designed as a library, of which N terminal end was fluorescence labelled. Decrease of fluorescence intensity of each peptide was quantitatively determined and used as a training data for Random Forest (RF) modeling. All of dipeptides bond as a protease substrate were included in 1990 tetramer peptides. As an explanatory variable for model construction, 532 parameters were prepared and those were included not only appearance of amino acid residue or dipeptides but also positioning parameters (PP) or global parameters (GP) to explain the peptide property. Trypsin for biochemistry grade was used for hydrolysis. After digesting at pH 8, the histogram of digestion ratio was appeared that tetramers with arginine residue (R) and lysine residue (K) could be expectedly digested at higher ratio compared with tetramers without R or K. Constructed pH 8-RF model showed 77 % of prediction accuracy. The explanatory parameters with top 10 higher importance in pH 8-RF model were both of appearance of K and 9 GPs. GPs were including 4 isoelectric parameters and 1 polarity parameter, of which K or R residue have relatively large value. Digestion site of α-lactalbumin was determined using pH 8-RF model and the protein was actually digested by trypsin. When the hydrolysate was analyzed by LC-MS/MS, 42 peptides were identified. Fourteen sites among 19 predicted digestion sites were coincided with each other. Many peptide fragments digested at 69Y-70G site or 50F-51H site was detected and it was strongly suggested to be digested by chymotrypsin as a contaminated protease. These sites were fairly predicted by constructed RF model. In addition, trypsin digestion at pH 5 was carried out to investigate the effect of pH decrease on trypsin digestion. The prediction accuracy of pH 5-RF model was only 59 %. The parameters with top 10 higher importance in pH 5-RF model were including 4 GPs which was the same with 4 isoelectric parameter in pH 8 model. Twenty digestion sites of α-lactalbumin were predicted from pH 5-RF model. α-lactalbumin was digested at pH 5 by trypsin, and 64 peptides were identified. The predicted sites were compared with identified fragments at the region from 48T to 77K in which a lot of fragments were detected. It was concluded that constructed model could fairly predict some of newly identified sites and could roughly grasp slight modification of digestion site by pH change. The proposed methodology, which contained 1990 kinds of tetramer peptides as a library, 532 explanatory parameters and RF model, was effective for prediction of protease digestion site.

DOI： 10.34565/seibutsukogaku.100.10_528

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ACS Omega  

We established a method for synthesizing a free cyclic peptide library via peptide array synthesis to demonstrate the sequence activity of cyclic peptides. Variants of the cyclic nonapeptide oxytocin (OXT) were synthesized via residue substitution. Natural amino acids (AAs) were classified into eight groups based on their physical properties and the size of their side chains, and a representative AA from each group was selected for residue substitution. All OXT variants were systematically evaluated for agonist/antagonist activity. Consequently, no improvement in agonist activity was observed, although substitution of the P4 and P8 residues resulted in decreased activity due to AA substitution. A few OXT variants exhibited antagonistic activity. In particular, the variants with P2 Leu residue substitution (Y2L) and Phe substitutions at residues 4 (Q4F), 5 (N5F), and 7 (P7F) showed high antagonistic activity. Variant Y2W was found to have the highest inhibitory effect, with a dissociation constant of 44 nM, which was comparable to that of the commercial antagonist atosiban (21 nM). Therefore, a free cyclic peptide library constructed via substitution with a natural AA residue was confirmed to be a powerful tool for bioactive peptide screening.

DOI： 10.1021/acsomega.1c04982

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Pharmaceutical Society of Japan  

DOI： 10.1248/bpb.b21-00269

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Foods  

Recently, many bioactive peptides have been identified using bioinformatics tools. Previously, our group developed a method to screen dual-functional peptides that have direct intestinal delivery with porous silica gel and bile acid micelle disruption. However, newly designed peptides were not found in any storage protein. Therefore, in this study, in silico screening was performed using a 350,000 edible peptide library consisting of 4-to 7-mer independent peptides. As an initial screening, all edible peptides were applied to the random forest model to select predicted positive peptides. For a second screening, the peptides were assessed for the possibility of intestinal delivery using a 3D color map. From this approach, three novel dual-functional peptides, VYVFDE, WEFIDF, and VEEFYC were identified, and all of them were derived from storage proteins (legumin, myosin, and 11S globulin). In particular, VEEFYCS, in which a serine residue (S) is added to VEEFYC, was assumed to be released by thermolysin from the 11S-globulin derived from Ginkgo biloba by LC-MS/MS analysis. VEEFYCS was found to have suitable direct intestinal delivery and bile acid micelle disruption activity.

DOI： 10.3390/foods10102496

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Language：English   Publishing type：Research paper (scientific journal)  

Electric pulse-stimulated C2C12 myotubes are gaining interest in the field of muscle physiology and biotechnology because electric pulse stimulation (EPS) enhances sarcomere structure development and active tension generation capability. Recently, we found that termination of EPS results in the rapid loss of active tension generation accompanied by disassembly of the sarcomere structure, which may represent an in vitro muscle atrophy model. To elucidate the molecular mechanism underlying this rapid loss of active tension generation and sarcomere structure disassembly after termination of EPS, we performed transcriptomic analysis using microarray. After termination of EPS, 74 genes were upregulated and 120 genes were downregulated after 30 min; however, atrophy-related genes were not found among these genes. To further assess the effect of EPS on gene expression, we re-applied EPS after its termination for 8 h and searched for genes whose expression was reversed. Four genes were upregulated by termination of EPS and downregulated by the re-application of EPS, whereas two genes were downregulated by termination of EPS and upregulated by the re-application of EPS. Although none of these genes were atrophy- or hypertrophy-related, the results presented in this study will contribute to the understanding of gene expression changes that mediate rapid loss of active tension generation and sarcomere structure disassembly following termination of EPS in C2C12 myotubes.

DOI： 10.1016/j.jbiosc.2021.06.016

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Biotechnology Letters  

Objectives: To develop a simple pectin-degrading microorganism screening method. Results: We developed a method utilizing the phenomenon whereby cooling an alkaline agar medium containing pectin causes the agar to become cloudy. This highly simplified method involves culturing the microorganisms on pectin-containing agar medium until colony formation is observed, and subsequent overnight cooling of the agar medium to 4 °C. Using this simple procedure, we successfully identified pectin-degrading microorganisms by observing colonies with halos on the clouded agar medium. We used alkaline pectinase and Bacillus halodurans, which is known to secrete alkaline pectinase, to establish the screening method. We demonstrated the screening of pectin-degrading microorganisms using the developed method and successfully isolated pectin-degrading microorganisms (Paenibacillus sp., Bacillus clausii, and Bacillus halodurans) from a soil sample. Conclusions: The developed method is useful for identifying pectin-degrading microorganisms.

DOI： 10.1007/s10529-021-03162-6

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Scientific Reports  

Bioactive peptides (BPs) are protein fragments that exhibit a wide variety of physicochemical properties, such as basic, acidic, hydrophobic, and hydrophilic properties; thus, they have the potential to interact with a variety of biomolecules, whereas neither carbohydrates nor fatty acids have such diverse properties. Therefore, BP is considered to be a new generation of biologically active regulators. Recently, some BPs that have shown positive benefits in humans have been screened from edible proteins. In the present study, a new BP screening method was developed using BIOPEP-UWM and machine learning. Training data were initially obtained using high-throughput techniques, and positive and negative datasets were generated. The predictive model was generated by calculating the explanatory variables of the peptides. To understand both site-specific and global characteristics, amino acid features (for site-specific characteristics) and peptide global features (for global characteristics) were generated. The constructed models were applied to the peptide database generated using BIOPEP-UWM, and bioactivity was predicted to explore candidate bile acid-binding peptides. Using this strategy, seven novel bile acid-binding peptides (VFWM, QRIFW, RVWVQ, LIRYTK, NGDEPL, PTFTRKL, and KISQRYQ) were identified. Our novel screening method can be easily applied to industrial applications using whole edible proteins. The proposed approach would be useful for identifying bile acid-binding peptides, as well as other BPs, as long as a large amount of training data can be obtained.

DOI： 10.1038/s41598-021-95461-1

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Language：English   Publishing type：Research paper (scientific journal)  

Engineered three-dimensional models of neuromuscular tissues are promising for use in mimicking their disorder states in vitro. Although several models have been developed, it is still challenging to mimic the physically separated structures of motor neurons (MNs) and skeletal muscle (SkM) fibers in the motor units in vivo. In this study, we aimed to develop microdevices for precisely compartmentalized coculturing of MNs and engineered SkM tissues. The developed microdevices, which fit a well of 24 well plates, had a chamber for MNs and chamber for SkM tissues. The two chambers were connected by microtunnels for axons, permissive to axons but not to cell bodies. Human iPSC (hiPSC)-derived MN spheroids in one chamber elongated their axons into microtunnels, which reached the tissue-engineered human SkM in the SkM chamber, and formed functional neuromuscular junctions with the muscle fibers. The cocultured SkM tissues with MNs on the device contracted spontaneously in response to spontaneous firing of MNs. The addition of a neurotransmitter, glutamate, into the MN chamber induced contraction of the cocultured SkM tissues. Selective addition of tetrodotoxin or vecuronium bromide into either chamber induced SkM tissue relaxation, which could be explained by the inhibitory mechanisms. We also demonstrated the application of chemical or mechanical stimuli to the middle of the axons of cocultured tissues on the device. Thus, compartmentalized neuromuscular tissue models fabricated on the device could be used for phenotypic screening to evaluate the cellular type specific efficacy of drug candidates and would be a useful tool in fundamental research and drug development for neuromuscular disorders.

DOI： 10.1039/d1lc00048a

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Applied Physics Express  

We present a conceptual design to generate and deliver nanoparticles in one unique system based on electrohydrodynamic atomisation (EHDA) without the restriction of the collector. The present EHDA bipolar configuration consists of a capillary nozzle and a pin, both act as emitters and as the reference electrodes of each other. Under an applied voltage, the capillary nozzle sprays droplets while the pin generates ion wind via corona discharge. During spraying process, droplets' charge is significantly reduced by interacting with counter ions and propelled away from the electrodes by the momentum of ion winds accumulated from corona discharge. Thus, the present technique can yield promising applications in effective respiratory delivery of nanomedicine. © 2021 The Japan Society of Applied Physics.

DOI： 10.35848/1882-0786/abf36b

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Biochemical and Biophysical Research Communications  

DOI： 10.1016/j.bbrc.2021.02.142

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Journal of Peptide Science  

DOI： 10.1002/psc.3287

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Kagaku Kogaku Ronbunshu  

To find FFAR1-activating peptides consisting of natural amino acid residues, molecular structure analysis was performed by hierarchical clustering using extraction of physicochemical parameters. When 3 mer to 6 mer peptide libraries were prepared and the molecular structure of each was compared with 5 chemical agonists, 12 peptides with high correlation coefficients of physicochemical parameters were found from the 6 mer peptide library. these peptides were then synthesized by Fmoc solid phase synthesis, and their activity was assessed by TGFα shedding assay using FFAR1-expressing HEK293. As a result, two peptides, GCGGSS and GASGCC, were identified as peptide agonists with relatively high activity. Although these peptides showed approximately one fithh of the activity of chemical agonist GW9508, they are the first examples that we know of FFAR1-activating peptides consisting of natural amino acid residues.

DOI： 10.1252/kakoronbunshu.47.64

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Seibutsu-kogaku Kaishi  

DOI： 10.34565/seibutsukogaku.99.3_122

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Publishing type：Research paper (scientific journal)   Publisher：ACS Biomaterials Science and Engineering  

DOI： 10.1021/acsbiomaterials.0c01010

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Publishing type：Research paper (scientific journal)   Publisher：Journal of Bioscience and Bioengineering  

DOI： 10.1016/j.jbiosc.2020.07.006

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Publishing type：Research paper (scientific journal)   Publisher：Advanced Biosystems  

DOI： 10.1002/adbi.202000121

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DOI： 10.1016/j.jbiosc.2020.02.008

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DOI： 10.1007/s00449-015-1412-9

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DOI： 10.1016/j.jbiosc.2019.11.013

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DOI： 10.1371/journal.pone.0228203

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Publishing type：Research paper (scientific journal)   Publisher：Biological and Pharmaceutical Bulletin  

DOI： 10.1248/BPB.B20-00141

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Publishing type：Research paper (scientific journal)   Publisher：Journal of Bioscience and Bioengineering  

DOI： 10.1016/j.jbiosc.2020.11.014

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DOI： 10.1007/s13206-018-3202-3

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DOI： 10.1089/ten.tea.2018.0063

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DOI： 10.1016/j.jbiosc.2018.08.006

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DOI： 10.1089/ten.TEA.2018.0063

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DOI： 10.1016/j.piosc.2018.08.006

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DOI： 10.3136/fstr.25.179

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DOI： 10.1016/j.jbiosc.2019.02.005

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DOI： 10.1016/j.jbiosc.2019.01.001

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DOI： 10.1016/j.reth.2018.06.001

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DOI： 10.1038/s41598-018-30936-2

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DOI： 10.1038/s41598-018-29345-2

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DOI： 10.1016/j.jbiosc.2017.11.012

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DOI： 10.1016/j.jconrel.2017.12.006

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DOI： 10.1248/bpb.b18-00094

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DOI： 10.1080/10717544.2017.1333171

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DOI： 10.1016/j.jbiosc.2017.04.006

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DOI： 10.1002/biot.201600513

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DOI： 10.1002/bit.26307

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DOI： 10.3390/bioengineering4020056

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DOI： 10.1016/j.jbiosc.2016.12.015

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DOI： 10.1248/bpb.b16-00833

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DOI： DOI: 10.1002/adhm.201300629

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Event date： 2020.12

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Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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Event date： 2020.8

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Country：Japan  

Event date： 2019.12

Language：English   Presentation type：Oral presentation (invited, special)  

Country：Australia  

Event date： 2019.12

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2019.9

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2019.3

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2019.1

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2019.1

Language：English   Presentation type：Oral presentation (general)  

Event date： 2018.1

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2018.1

Language：English   Presentation type：Oral presentation (general)  

Event date： 2017.10

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2017.9

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2017.8

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2017.7

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2017.1

Language：Japanese   Presentation type：Poster presentation  

Country：Japan  

Event date： 2017.1

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2017.1

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2017.1

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Event date： 2016.10

Language：English   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2016.10

Language：English   Presentation type：Oral presentation (invited, special)  

Country：Korea, Republic of  

Event date： 2016.6

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2016.5

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2016.1

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2015.11

Language：English   Presentation type：Oral presentation (general)  

Country：Malaysia  

Event date： 2015.10

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2015.10

Language：English   Presentation type：Poster presentation  

Country：United States  

Event date： 2015.8

Language：Japanese   Presentation type：Poster presentation  

Country：Japan  

Event date： 2015.8

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2015.6

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2014.11

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2014.11

Language：English   Presentation type：Oral presentation (general)  

Country：Taiwan, Province of China  

Event date： 2013.8

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Venue：長崎大学   Country：Japan  

Event date： 2013.7

Language：English   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2013.3

Language：English   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2012.12

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2012.11

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2012.11

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2012.10 - 2012.11

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2012.10

Language：English   Presentation type：Oral presentation (invited, special)  

Country：United States  

Event date： 2011.10

Language：English   Presentation type：Oral presentation (general)  

Country：United States  

Event date： 2011.6

Language：English   Presentation type：Oral presentation (general)  

Country：China  

Event date： 2011.1

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2010.10

Language：English   Presentation type：Poster presentation  

Country：Netherlands  

Event date： 2010.10

Language：English   Presentation type：Poster presentation  

Country：Japan  

Event date： 2010.10

Language：English   Presentation type：Poster presentation  

Country：Netherlands  

Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

Application no：特願2018-037098  Date applied：2018.3

Country of applicant：Domestic  

Application no：特願2017-150361  Date applied：2017.8

Country of applicant：Domestic  

Application no：特願2017-150361  Date applied：2017.8

Country of applicant：Domestic  

Application no：特願2017-149479  Date applied：2017.8

Country of applicant：Domestic  

Application no：特願2017-149479  Date applied：2017.8

Country of applicant：Domestic  

Application no：特願2017-146141  Date applied：2017.7

Country of applicant：Domestic  

Application no：特願2017-146141  Date applied：2017.7

Country of applicant：Domestic  

Application no：特願2017-92447  Date applied：2017.5

Country of applicant：Domestic  

Application no：特願2017-92447  Date applied：2017.5

Country of applicant：Domestic  

Application no：特願2016-185171  Date applied：2016.9

Country of applicant：Domestic  

Application no：特願2015-155312  Date applied：2015.8

Country of applicant：Domestic  

Applicant：国立大学法人京都大学, 学校法人立命館

Application no：特願2011-259007  Date applied：2011.11

Patent/Registration no：特許第5930364号  Date registered：2016.5 

Country of applicant：Domestic  

Applicant：株式会社豊田中央研究所

Application no：特願2010-247914  Date applied：2010.11

Patent/Registration no：特許第5549547号  Date registered：2014.5 

Country of applicant：Domestic  

Applicant：国立大学法人京都大学, 学校法人立命館

Application no：特願2010-238885  Date applied：2010.10

Country of applicant：Domestic  

Application no：12/659120  Date applied：2010.2

Announcement no：US 2010/0216242 

Country of applicant：Foreign country  

Applicant：株式会社豊田中央研究所

Application no：特願2009-132301  Date applied：2009.6

Patent/Registration no：特許第4483994号  Date registered：2010.4 

Country of applicant：Domestic  

Applicant：株式会社豊田中央研究所

Application no：特願2008-180547  Date applied：2008.7

Patent/Registration no：特許第4553038号  Date registered：2010.7 

Country of applicant：Domestic  

Author：Other 

Author：Other 

Author：Other 

Author：Other 

Author：Other 

Author：Other 

Author：Other