Updated on 2022/04/23

写真a

 
ISHIKAWA Yuki
 
Organization
Graduate School of Science Lecturer
Graduate School
Graduate School of Science
Undergraduate School
School of Science Department of Biological Science
Title
Lecturer

Degree 1

  1. PhD ( 2011.3   Hokkaido University ) 

Research Interests 8

  1. flower visiting

  2. Drosophila simulans

  3. Drosophila melanogaster

  4. Drosophila elegans

  5. mating preference

  6. behavioral evolution

  7. courtship behavior

  8. fruit fly

Research Areas 8

  1. Life Science / Evolutionary biology

  2. Life Science / Neuroscience-general

  3. Life Science / Ecology and environment

  4. Life Science / Animal physiological chemistry, physiology and behavioral biology

  5. Life Science / Neuroscience-general

  6. Life Science / Ecology and environment

  7. Life Science / Animal physiological chemistry, physiology and behavioral biology

  8. Life Science / Evolutionary biology

▼display all

Research History 4

  1. Nagoya University   Graduate School of Science   Lecturer

    2017.11

  2. Nagoya University   Graduate School of Science   Designated assistant professor

    2013.8 - 2017.10

  3. JSPS postdoc

    2011.4 - 2013.7

  4. JSPS Fellowship

    2008.4 - 2011.3

Education 1

  1. Hokkaido University   Graduate School of Environmental Science

    2002.4 - 2011.3

Professional Memberships 5

  1. SOCIETY OF EVOLUTIONARY STUDIES, JAPAN

  2. THE JAPAN NEUROSCIENCE SOCIETY

  3. ECOLOGICAL SOCIETY OF JAPAN

  4. THE JAPANESE SOCIETY FOR COMPARATIVE PHYSIOLOGY AND BIOCHEMISTRY

  5. THE ZOOLOGICAL SOCIETY OF JAPAN

Committee Memberships 19

  1. 日本比較生理生化学会   2022年度賞審査委員会(原富之賞担当)  

    2022.4   

  2. 日本比較生理生化学会   評議委員  

    2022.4   

  3. 日本進化学会   副編集委員長  

    2021.3   

  4. 日本進化学会   理事(会計)  

    2018.4   

      More details

    Committee type:Academic society

  5. 日本進化学会   代議員  

    2018.4   

      More details

    Committee type:Academic society

  6. 日本比較生理生化学会   幹事(行事担当)  

    2018.4 - 2021.3   

  7. 日本比較生理生化学会   2018年度賞審査委員会(原富之賞担当担当)  

    2018.4 - 2019.4   

  8. 日本比較生理生化学会   2018年度賞審査委員会(原富之賞担当担当)  

    2018.4 - 2019.3   

      More details

    Committee type:Academic society

  9. 日本進化学会   理事(会計)  

    2018.3   

  10. 日本比較生理生化学会   幹事(行事担当)  

    2018   

      More details

    Committee type:Academic society

  11. 日本比較生理生化学会   2017年度賞審査委員会(吉田奨励賞担当)  

    2017.4 - 2018.4   

  12. 日本比較生理生化学会   2017年度賞審査委員会(吉田奨励賞担当)  

    2017.4 - 2018.3   

      More details

    Committee type:Academic society

  13. 日本進化学会   編集委員  

    2016.4   

  14. 日本比較生理生化学会   評議委員  

    2016.4   

      More details

    Committee type:Academic society

  15. 日本比較生理生化学会   行事委員  

    2016.4   

      More details

    Committee type:Academic society

  16. 日本進化学会   編集委員  

    2016.4   

      More details

    Committee type:Academic society

  17. 日本比較生理生化学会   行事委員  

    2016.4   

  18. 生態進化発生コロキウム   幹事  

    2013   

      More details

    Committee type:Academic society

  19. Evo-Devo青年の会   幹事  

       

      More details

    Committee type:Academic society

▼display all

Awards 5

  1. Poster Award for Excellence, The 46th Naito Conference

    2018.10   The Naito Foundation  

    Yuki Ishikawa

  2. 第27回高遠分子細胞生物学シンポジウム優秀ポスター賞

    2015.8   高遠分子細胞生物学シンポジウム  

    石川 由希

  3. Yoshida Young Research Award

    2014.7   The Japanese Society of Comparative Physiology and Biochemistry  

    Yuki Ishikawa

  4. Heart Prize 2007

    2007.11   Heart Co. Ltd.  

    Yuki Ishikawa

  5. Award for best poster prize

    2006.3   The Ecological Society of Japan  

    Yuki Ishikawa

 

Papers 36

  1. Screening of upregulated genes induced by high density in the vetch aphid Megoura crassicauda. Reviewed International journal

    Asano Ishikawa, Yuki Ishikawa, Yasukazu Okada, Satoshi Miyazaki, Hitoshi Miyakawa, Shigeyuki Koshikawa, Jennifer A Brisson, Toru Miura

    Journal of experimental zoology. Part A, Ecological genetics and physiology   Vol. 317 ( 3 ) page: 194 - 203   2012.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:3  

    Aphids exhibit several polyphenisms in which discontinuous, alternative phenotypes are produced depending on environmental conditions. One representative example is the wing polyphenism, where winged and wingless females are produced through parthenogenesis. Previous work has shown that, in some aphid species, the density condition sensed by the mother aphid determines the developmental fate of embryos in her ovary, with high densities leading to winged progeny and low densities to wingless progeny. However, little is known about the molecular and physiological mechanisms underlying the wing polyphenism. To identify genes involved in the wing-morph determination in the vetch aphid, Megoura crassicauda, we compared maternal and embryonic transcripts between high- and low-density conditions using differential display, followed by quantitative real-time PCR (qRT-PCR). Under the high-density condition, two genes (Uba1 and Naca) were found to be upregulated in maternal tissues without ovaries, while one gene (ClpP) was upregulated in ovaries containing embryos. Uba1 and Naca encode factors that function in protein modification or transcriptional/translational regulation, respectively. In addition to differential display, candidate gene approaches focusing on morphogenetic and endocrine genes, i.e., wg, dpp, ap, hh, InR, IRS, Foxo, EcR, and USP, were also carried out. We found that wg was upregulated in maternal tissues under the high-density condition. The identified genes from both approaches are candidates for further study of their involvement in the transduction of density signals in mother aphids and/or the initial process of wing differentiation in embryos.

    DOI: 10.1002/jez.1713

    Web of Science

    PubMed

  2. Wiring patterns from auditory sensory neurons to the escape and song-relay pathways in fruit flies Reviewed International journal

    Hyunsoo Kim, Mihoko Horigome, Yuki Ishikawa, Feng Li, J. Scott Lauritzen, Gwyneth Card, Davi D. Bock, Azusa Kamikouchi

    JOURNAL OF COMPARATIVE NEUROLOGY   Vol. 528 ( 12 ) page: 2068 - 2098   2020.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    Many animals rely on acoustic cues to decide what action to take next. Unraveling the wiring patterns of the auditory neural pathways is prerequisite for understanding such information processing. Here, we reconstructed the first step of the auditory neural pathway in the fruit fly brain, from primary to secondary auditory neurons, at the resolution of transmission electron microscopy. By tracing axons of two major subgroups of auditory sensory neurons in fruit flies, low-frequency tuned Johnston's organ (JO)-B neurons and high-frequency tuned JO-A neurons, we observed extensive connections from JO-B neurons to the main second-order neurons in both the song-relay and escape pathways. In contrast, JO-A neurons connected strongly to a neuron in the escape pathway. Our findings suggest that heterogeneous JO neuronal populations could be recruited to modify escape behavior whereas only specific JO neurons contribute to courtship behavior. We also found that all JO neurons have postsynaptic sites at their axons. Presynaptic modulation at the output sites of JO neurons could affect information processing of the auditory neural pathway in flies.

    DOI: 10.1002/cne.24877

    Web of Science

    PubMed

  3. Behavioral Evolution of Drosophila: Unraveling the Circuit Basis

    Kosei Sato, Ryoya Tanaka, Yuki Ishikawa, Daisuke Yamamoto

    GENES   Vol. 11 ( 2 )   2020.2

     More details

    Language:English   Publisher:MDPI  

    Behavior is a readout of neural function. Therefore, any difference in behavior among different species is, in theory, an outcome of interspecies diversification in the structure and/or function of the nervous system. However, the neural diversity underlying the species-specificity in behavioral traits and its genetic basis have been poorly understood. In this article, we discuss potential neural substrates for species differences in the courtship pulse song frequency and mating partner choice in the Drosophila melanogaster subgroup. We also discuss possible neurogenetic mechanisms whereby a novel behavioral repertoire emerges based on the study of nuptial gift transfer, a trait unique to D. subobscura in the genus Drosophila. We found that the conserved central circuit composed primarily of fruitless-expressing neurons (the fru-circuit) serves for the execution of courtship behavior, whereas the sensory pathways impinging onto the fru-circuit or the motor pathways downstream of the fru-circuit are susceptible to changes associated with behavioral species differences.

    DOI: 10.3390/genes11020157

    Web of Science

    Scopus

    PubMed

  4. Stereotyped Combination of Hearing and Wind/Gravity-Sensing Neurons in the Johnston's Organ of Drosophila Reviewed International journal

    Yuki Ishikawa, Mao Fujiwara, Junlin Wong, Akari Ura, Azusa Kamikouchi

    FRONTIERS IN PHYSIOLOGY   Vol. 10   page: 1552 - 1552   2020.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:FRONTIERS MEDIA SA  

    The antennal ear of the fruit fly, called the Johnston's organ (JO), detects a wide variety of mechanosensory stimuli, including sound, wind, and gravity. Like many sensory cells in insect, JO neurons are compartmentalized in a sensory unit (i.e., scolopidium). To understand how different subgroups of JO neurons are organized in each scolopidial compartment, we visualized individual JO neurons by labeling various subgroups of JO neurons in different combinations. We found that vibration-sensitive (or deflection-sensitive) neurons rarely grouped together in a single scolopidial compartment. This finding suggests that JO neurons are grouped in stereotypical combinations each with a distinct response property in a scolopidium.

    DOI: 10.3389/fphys.2019.01552

    Web of Science

    PubMed

  5. A single male auditory response test to quantify auditory behavioral responses in Drosophila melanogaster Reviewed

    Yuki Ishikawa, Natsuki Okamoto, Yusuke Yoneyama, Naoki Maeda, Azusa Kamikouchi

    JOURNAL OF NEUROGENETICS   Vol. 33 ( 2 ) page: 64 - 74   2019.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS LTD  

    Many animals utilize auditory signals to communicate with conspecific individuals. During courtship, males of the fruit fly Drosophila melanogaster and related species produce a courtship song comprised of sine and pulse songs by vibrating their wings. The pulse song increases female receptivity and male courtship activity, indicating that it functions as a sexual signal. One song parameter, interpulse interval (IPI), varies among closely related species. In D. melanogaster, a song with a conspecific IPI induces a stronger behavioral response than heterospecific songs, indicating the ability of the flies to discriminate conspecific IPI. Traditionally, the fly's response to the song is measured under grouped conditions, in which the effect of sensory modalities other than audition cannot be excluded. Here, to quantify the individual ability to discriminate a conspecific song, we systematically analyzed the auditory response of single male flies to sound with various parameters. Moreover, we applied this method, termed SMART (Single Male Auditory Response Test), to two sister species for potential application in a comparative approach. By quantifying the locomotor activity of single D. melanogaster males during sound exposure, we detected increased locomotor activity in response to pulse songs, but not to white noise or pure tone. The conspecific song evoked stronger response than the heterospecific songs, and ablation of their antennal receivers severely suppressed the locomotor increase. A pulse song with a small IPI variation evoked a continuous response, while the response to songs with highly variable IPIs tends to be rapidly decayed. This provides the first evidence that fruit flies discriminate IPI variations, which possibly inform the age and social contexts of the singer. Sister species, D. sechellia, exhibited a locomotor response to pulse song, while D. simulans exhibited no behavioral response. This suggests that auditory and other stimuli that elicit this behavioral response are diversified among Drosophila species.

    DOI: 10.1080/01677063.2019.1611805

    Web of Science

    Scopus

    PubMed

  6. STEFTR: A Hybrid Versatile Method for State Estimation and Feature Extraction From the Trajectory of Animal Behavior. Reviewed International journal

    Shuhei J Yamazaki, Kazuya Ohara, Kentaro Ito, Nobuo Kokubun, Takuma Kitanishi, Daisuke Takaichi, Yasufumi Yamada, Yosuke Ikejiri, Fumie Hiramatsu, Kosuke Fujita, Yuki Tanimoto, Akiko Yamazoe-Umemoto, Koichi Hashimoto, Katsufumi Sato, Ken Yoda, Akinori Takahashi, Yuki Ishikawa, Azusa Kamikouchi, Shizuko Hiryu, Takuya Maekawa, Koutarou D Kimura

    Frontiers in neuroscience   Vol. 13   page: 626 - 626   2019

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Animal behavior is the final and integrated output of brain activity. Thus, recording and analyzing behavior is critical to understand the underlying brain function. While recording animal behavior has become easier than ever with the development of compact and inexpensive devices, detailed behavioral data analysis requires sufficient prior knowledge and/or high content data such as video images of animal postures, which makes it difficult for most of the animal behavioral data to be efficiently analyzed. Here, we report a versatile method using a hybrid supervised/unsupervised machine learning approach for behavioral state estimation and feature extraction (STEFTR) only from low-content animal trajectory data. To demonstrate the effectiveness of the proposed method, we analyzed trajectory data of worms, fruit flies, rats, and bats in the laboratories, and penguins and flying seabirds in the wild, which were recorded with various methods and span a wide range of spatiotemporal scales-from mm to 1,000 km in space and from sub-seconds to days in time. We successfully estimated several states during behavior and comprehensively extracted characteristic features from a behavioral state and/or a specific experimental condition. Physiological and genetic experiments in worms revealed that the extracted behavioral features reflected specific neural or gene activities. Thus, our method provides a versatile and unbiased way to extract behavioral features from simple trajectory data to understand brain function.

    DOI: 10.3389/fnins.2019.00626

    Web of Science

    PubMed

  7. GABAergic Local Interneurons Shape Female Fruit Fly Response to Mating Songs Reviewed

    Daichi Yamada, Hiroshi Ishimoto, Xiaodong Li, Tsunehiko Kohashi, Yuki Ishikawa, Azusa Kamikouchi

    JOURNAL OF NEUROSCIENCE   Vol. 38 ( 18 ) page: 4329 - 4347   2018.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SOC NEUROSCIENCE  

    Many animals use acoustic signals to attract a potential mating partner. In fruit flies (Drosophila melanogaster), the courtship pulse song has a species-specific interpulse interval (IPI) that activates mating. Although a series of auditory neurons in the fly brain exhibit different tuning patterns to IPIs, it is unclear how the response of each neuron is tuned. Here, we studied the neural circuitry regulating the activity of antennal mechanosensory and motor center (AMMC)-B1 neurons, key secondary auditory neurons in the excitatory neural pathway that relay song information. By performing Ca2+ imaging in female flies, we found that the IPI selectivity observed in AMMC-B1 neurons differs from that of upstream auditory sensory neurons [Johnston's organ (JO)-B]. Selective knock-down of a GABA(A) receptor subunit in AMMC-B1 neurons increased their response to short IPIs, suggesting that GABA suppresses AMMC-B1 activity at these IPIs. Connection mapping identified two GABAergic local interneurons that synapse with AMMC-B1 and JO-B. Ca2(+) imaging combined with neuronal silencing revealed that these local interneurons, AMMC-LN and AMMC-B2, shape the response pattern of AMMC-B1 neurons at a 15ms IPI. Neuronal silencing studies further suggested that both GABAergic local interneurons suppress the behavioral response to artificial pulse songs in flies, particularly those with a 15ms IPI. Altogether, we identified a circuit containing two GABAergic local interneurons that affects the temporal tuning of AMMC-B1 neurons in the song relay pathway and the behavioral response to the courtship song. Our findings suggest that feedforward inhibitory pathways adjust the behavioral response to courtship pulse songs in female flies.

    DOI: 10.1523/JNEUROSCI.3644-17.2018

    Web of Science

    PubMed

  8. Anatomic and Physiologic Heterogeneity of Subgroup-A Auditory Sensory Neurons in Fruit Flies Reviewed

    Yuki Ishikawa, Natsuki Okamoto, Mizuki Nakamura, Hyunsoo Kim, Azusa Kamikouchi

    Frontiers in Neural Circuits     2017.6

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: /10.3389/fncir.2017.00046

    PubMed

  9. Anatomic and Physiologic Heterogeneity of Subgroup-A Auditory Sensory Neurons in Fruit Flies

    Ishikawa Yuki, Okamoto Natsuki, Nakamura Mizuki, Kim Hyunsoo, Kamikouchi Azusa

    FRONTIERS IN NEURAL CIRCUITS   Vol. 11   2017.6

  10. The function of appendage patterning genes in mandible development of the sexually dimorphic stag beetle Reviewed

    Hiroki Gotoh, Robert A. Zinna, Yuki Ishikawa, Hitoshi Miyakawa, Asano Ishikawa, Yasuhiro Sugime, Douglas J. Emlen, Laura C. Lavine, Toru Miura

    DEVELOPMENTAL BIOLOGY   Vol. 422 ( 1 ) page: 24 - 32   2017.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    One of the defining features of the evolutionary success of insects is the morphological diversification of their appendages, especially mouthparts. Although most insects share a common mouthpart ground plan, there is remarkable diversity in the relative size and shapes of these appendages among different insect lineages. One of the most prominent examples of mouthpart modification can be found in the enlargement of mandibles in stag beetles (Coleoptera, Insecta). In order to understand the proximate mechanisms of mouthpart modification, we investigated the function of appendage-patterning genes in mandibular enlargement during extreme growth of the sexually dimorphic mandibles of the stag beetle Cyclommatus metallifer. Based on knowledge from Drosophila and Tribolium studies, we focused on seven appendage patterning genes (Distal-less (Dll), aristaless (al), dachshund (dac), homothorax (hth), Epidermal growth factor receptor (Egfr), escargot (esg), and Keren (Krn). In order to characterize the developmental function of these genes, we performed functional analyses by using RNA interference (RNAi). Importantly, we found that RNAi knockdown of dac resulted in a significant mandible size reduction in males but not in female mandibles. In addition to reducing the size of mandibles, dac knockdown also resulted in a loss of the serrate teeth structures on the mandibles of males and females. We found that al and hilt play a significant role during morphogenesis of the large male specific inner mandibular tooth. On the other hand, knockdown of the distal selector gene Dll did not affect mandible development, supporting the hypothesis that mandibles likely do not contain the distal-most region of the ancestral appendage and therefore co-option of Dll expression is unlikely to be involved in mandible enlargement in stag beetles. In addition to mandible development, we explored possible roles of these genes in controlling the divergent antennal morphology of Coleoptera.

    DOI: 10.1016/j.ydbio.2016.12.011

    Web of Science

    PubMed

  11. Neuronal mechanisms of evolution of species-specific pheromone preference in Drosophila

    Ishikawa Yuki, Kamikouchi Azusa, Yamamoto Daisuke

    GENES & GENETIC SYSTEMS   Vol. 91 ( 6 ) page: 325 - 325   2016.12

     More details

  12. Auditory system of fruit flies Reviewed

    Yuki Ishikawa, Azusa Kamikouchi

    HEARING RESEARCH   Vol. 338   page: 1 - 8   2016.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    The fruit fly, Drosophila melanogaster, is an invaluable model for auditory research. Advantages of using the fruit fly include its stereotyped behavior in response to a particular sound, and the availability of molecular-genetic tools to manipulate gene expression and cellular activity. Although the receiver type in fruit flies differs from that in mammals, the auditory systems of mammals and fruit flies are strikingly similar with regard to the level of development, transduction mechanism, mechanical amplification, and central projections. These similarities strongly support the use of the fruit fly to study the general principles of acoustic information processing. In this review, we introduce acoustic communication and discuss recent advances in our understanding on hearing in fruit flies.This article is part of a Special Issue entitled <Annual Reviews 2016>. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.heares.2015.10.017

    Web of Science

    PubMed

  13. Tyraminergic and Octopaminergic Modulation of Defensive Behavior in Termite Soldier. Reviewed International journal

    Yuki Ishikawa, Hitoshi Aonuma, Ken Sasaki, Toru Miura

    PloS one   Vol. 11 ( 5 ) page: e0154230   2016

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PUBLIC LIBRARY SCIENCE  

    In termites, i.e. a major group of eusocial insects, the soldier caste exhibits specific morphological characteristics and extremely high aggression against predators. Although the genomic background is identical to the other non-aggressive castes, they acquire the soldier-specific behavioral character during the course of caste differentiation. The high aggressiveness and defensive behavior is essential for colony survival, but the neurophysiological bases are completely unknown. In the present study, using the damp-wood termite Hodotermopsis sjostedti, we focused on two biogenic amines, octopamine (OA) and tyramine (TA), as candidate neuromodulators for the defensive behavior in soldiers. High-performance liquid chromatographic analysis revealed that TA levels in the brain and suboesophageal ganglion (SOG) and the OA level in brain were increased in soldiers than in pseudergates (worker caste). Immunohistochemical analysis revealed that TA/OA neurons that innervate specific areas, including the mandibular muscles, antennal nerve, central complex, suboesophageal ganglion, and thoracic and/or abdominal ganglia, were enlarged in a soldier-specific manner. Together with the results that pharmacological application of TA promoted the defensive behavior in pseudergates, these findings suggest that the increased TA/OA levels induce the higher aggressiveness and defensive behavior in termite soldiers. The projection targets of these soldier-specific enlarged TA/OA neurons may have important roles in the higher aggressiveness and defensive behavior of the termite soldiers, inducing the neuronal transition that accompanies external morphological changes.

    DOI: 10.1371/journal.pone.0154230

    Web of Science

    Scopus

    PubMed

  14. Physiological and neural mechanisms underlying the division of labor in termite

    Yuki ISHIKAWA

    Hikaku seiri seikagaku(Comparative Physiology and Biochemistry)   Vol. 33 ( 4 ) page: 191 - 202   2016

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:The Japanese Society for Comparative Physiology and Biochemistry  

    <p>Termites show a highly complex society, which is supported by the cooperation of the colony members with different morphology and behavior. These individuals, termed as castes, possess the identical genomic background with the other colony members, but specialized to particular tasks in their colony. For task specialization, the neural mechanism is required to differentiate in caste-specific manner. However, what mechanisms are underlying the task specialization, and how the complex sociality in termites was evolved from the ancestral solitary cockroach has been unrevealed.</p><p></p><p>In this review, I firstly provide an overview of the termite sociality, including the terminology, which is specific to social biology. Secondly, I introduce the developmental pathway and the regulatory mechanisms in which the colony members differentiate into castes in adapting to fluctuating environmental condition. Thirdly, recent advances in understanding the neural mechanisms underlying the termite task specialization are described. Finally, I will discuss how the termite sociality was evolved from the solitary ancestors with the modification of the physiological and neural mechanisms.</p>

    DOI: 10.3330/hikakuseiriseika.33.191

    CiNii Research

  15. Hearing in Drosophila

    Kamikouchi Azusa, Ishikawa Yuki

    INSECT HEARING: WITH 53 ILLUSTRATIONS   Vol. 55   page: 239 - 262   2016

  16. Identification of novel vibration- and deflection-sensitive neuronal subgroups in Johnston's organ of the fruit fly Reviewed

    Eriko Matsuo, Daichi Yamada, Yuki Ishikawa, Tomonori Asai, Hiroshi Ishimoto, Azusa Kamikouchi

    FRONTIERS IN PHYSIOLOGY   Vol. 5 ( 179 ) page: 179   2014.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:FRONTIERS MEDIA SA  

    The fruit fly Drosophila melanogaster responds behaviorally to sound, gravity, and wind. Johnston's organ (JO) at the antennal base serves as a sensory organ in the fruit fly to detect these mechanosensory stimuli. Among the five anatomically defined subgroups of sensory neurons in JO, subgroups A and B detect sound vibrations and subgroups C and E respond to static deflections, such as gravity and wind. The functions of subgroup-D JO neurons, however, remain unknown. In this study, we used molecular-genetic methods to explore the physiologic properties of subgroup-D JO neurons. Both vibrations and static deflection of the antennal receiver activated subgroup-D JO neurons. This finding clearly revealed that zone D in the antennal mechanosensory and motor center (AMMC), the projection target of subgroup-D JO neurons, is a primary center for antennal vibrations and deflection in the fly brain. We anatomically identified two types of interneurons downstream of subgroup-D JO neurons, AMMC local neurons (AM MC LNs), and AMMC D1 neurons. AMMC LNs are local neurons whose projections are confined within the AMMC, connecting zones B and D. On the other hand, AMMC D1 neurons have both local dendritic arborizations within the AMMC and descending projections to the thoracic ganglia, suggesting that AM MC D1 neurons are likely to relay information of the antennal movement detected by subgroup-D JO neurons from the AMMC directly to the thorax. Together, these findings provide a neural basis for how JO and its brain targets encode information of complex movements of the fruit fly antenna.

    DOI: 10.3389/fphys.2014.00179

    Web of Science

    PubMed

  17. Developmental Link between Sex and Nutrition; doublesex Regulates Sex-Specific Mandible Growth via Juvenile Hormone Signaling in Stag Beetles

    Gotoh Hiroki, Miyakawa Hitoshi, Ishikawa Asano, Ishikawa Yuki, Sugime Yasuhiro, Emlen Douglas J., Lavine Laura C., Miura Toru

    PLOS GENETICS   Vol. 10 ( 1 )   2014.1

  18. Developmental link between sex and nutrition; doublesex regulates sex-specific mandible growth via juvenile hormone signaling in stag beetles.

    Gotoh H, Miyakawa H, Ishikawa A, Ishikawa Y, Sugime Y, Emlen DJ, Lavine LC, Miura T

    PLoS genetics   Vol. 10 ( 1 ) page: e1004098   2014.1

  19. Developmental Link between Sex and Nutrition; doublesex Regulates Sex-Specific Mandible Growth via Juvenile Hormone Signaling in Stag Beetles Reviewed

    Hiroki Gotoh, Hitoshi Miyakawa, Asano Ishikawa, Yuki Ishikawa, Yasuhiro Sugime, Douglas J. Emlen, Laura C. Lavine, Toru Miura

    PLoS Genetics   Vol. 10 ( 1 ) page: e1004098   2014.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:1  

    Sexual dimorphisms in trait expression are widespread among animals and are especially pronounced in ornaments and weapons of sexual selection, which can attain exaggerated sizes. Expression of exaggerated traits is usually male-specific and nutrition sensitive. Consequently, the developmental mechanisms generating sexually dimorphic growth and nutrition-dependent phenotypic plasticity are each likely to regulate the expression of extreme structures. Yet we know little about how either of these mechanisms work, much less how they might interact with each other. We investigated the developmental mechanisms of sex-specific mandible growth in the stag beetle Cyclommatus metallifer, focusing on doublesex gene function and its interaction with juvenile hormone (JH) signaling. doublesex genes encode transcription factors that orchestrate male and female specific trait development, and JH acts as a mediator between nutrition and mandible growth. We found that the Cmdsx gene regulates sex differentiation in the stag beetle. Knockdown of Cmdsx by RNA-interference in both males and females produced intersex phenotypes, indicating a role for Cmdsx in sex-specific trait growth. By combining knockdown of Cmdsx with JH treatment, we showed that female-specific splice variants of Cmdsx contribute to the insensitivity of female mandibles to JH: knockdown of Cmdsx reversed this pattern, so that mandibles in knockdown females were stimulated to grow by JH treatment. In contrast, mandibles in knockdown males retained some sensitivity to JH, though mandibles in these individuals did not attain the full sizes of wild type males. We suggest that moderate JH sensitivity of mandibular cells may be the default developmental state for both sexes, with sex-specific Dsx protein decreasing sensitivity in females, and increasing it in males. This study is the first to demonstrate a causal link between the sex determination and JH signaling pathways, which clearly interact to determine the developmental fates and final sizes of nutrition-dependent secondary-sexual characters. © 2014 Gotoh et al.

    DOI: 10.1371/journal.pgen.1004098

    Scopus

    PubMed

  20. Developmental Link between Sex and Nutrition; doublesex Regulates Sex-Specific Mandible Growth via Juvenile Hormone Signaling in Stag Beetles Reviewed

    Hiroki Gotoh, Hitoshi Miyakawa, Asano Ishikawa, Yuki Ishikawa, Yasuhiro Sugime, Douglas J. Emlen, Laura C. Lavine, Toru Miura

    PLOS GENETICS   Vol. 10 ( 1 )   2014.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PUBLIC LIBRARY SCIENCE  

    Sexual dimorphisms in trait expression are widespread among animals and are especially pronounced in ornaments and weapons of sexual selection, which can attain exaggerated sizes. Expression of exaggerated traits is usually male-specific and nutrition sensitive. Consequently, the developmental mechanisms generating sexually dimorphic growth and nutrition-dependent phenotypic plasticity are each likely to regulate the expression of extreme structures. Yet we know little about how either of these mechanisms work, much less how they might interact with each other. We investigated the developmental mechanisms of sex-specific mandible growth in the stag beetle Cyclommatus metallifer, focusing on doublesex gene function and its interaction with juvenile hormone (JH) signaling. doublesex genes encode transcription factors that orchestrate male and female specific trait development, and JH acts as a mediator between nutrition and mandible growth. We found that the Cmdsx gene regulates sex differentiation in the stag beetle. Knockdown of Cmdsx by RNA-interference in both males and females produced intersex phenotypes, indicating a role for Cmdsx in sex-specific trait growth. By combining knockdown of Cmdsx with JH treatment, we showed that female-specific splice variants of Cmdsx contribute to the insensitivity of female mandibles to JH: knockdown of Cmdsx reversed this pattern, so that mandibles in knockdown females were stimulated to grow by JH treatment. In contrast, mandibles in knockdown males retained some sensitivity to JH, though mandibles in these individuals did not attain the full sizes of wild type males. We suggest that moderate JH sensitivity of mandibular cells may be the default developmental state for both sexes, with sex-specific Dsx protein decreasing sensitivity in females, and increasing it in males. This study is the first to demonstrate a causal link between the sex determination and JH signaling pathways, which clearly interact to determine the developmental fates and final sizes of nutrition-dependent secondary-sexual characters.

    DOI: 10.1371/journal.pgen.1004098

    Web of Science

  21. Developmental link between sex and nutrition; doublesex regulates sex-specific mandible growth via juvenile hormone signaling in stag beetles Reviewed

    Gotoh H, Miyakawa H, Ishikawa A, Ishikawa Y, Sugime Y, Emlen DJ, Lavine LC, Miura T

    PLoS Genetics   Vol. 10 ( 1 ) page: e1004098   2014

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  22. Genetic and Neural Bases for Species-Specific Behavior in Drosophila Species. Reviewed

    Yamamoto D, Ishikawa Y

      Vol. 27 ( 3 ) page: 130-142   2013.9

     More details

    Language:English  

    DOI: 10.3109/01677063.2013.800060

  23. Soldier morphogenesis in the damp-wood termite is regulated by the insulin signaling pathway. Reviewed International journal

    Akiko Hattori, Yasuhiro Sugime, Chifune Sasa, Hitoshi Miyakawa, Yuki Ishikawa, Satoshi Miyazaki, Yasukazu Okada, Richard Cornette, Laura Corley Lavine, Douglas J Emlen, Shigeyuki Koshikawa, Toru Miura

    Journal of experimental zoology. Part B, Molecular and developmental evolution   Vol. 320 ( 5 ) page: 295 - 306   2013.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Eusocial insects exhibit various morphological castes associated with the division of labor within a colony. Termite soldiers possess defensive traits including mandibles that are greatly exaggerated and enlarged, as compared to termite reproductives and workers. The enlarged mandibles of soldiers are known to result from dynamic morphogenesis during soldier differentiation that can be induced by juvenile hormone and its analogs. However, the detailed developmental mechanisms still remain unresolved. Because the insulin/insulin-like growth factor signaling (IIS) pathway has been shown to regulate the relative sizes of organs (i.e., allometry) in other insects, we examined the expression profiles of major IIS factors in the damp-wood termite Hodotermopsis sjostedti, during soldier differentiation. The relative expression patterns of orthologs for termite InR (HsjInR), PKB/Akt (HsjPKB/Akt), and FOXO (HsjFOXO) suggest that HsjInR and HsjPKB/Akt were up-regulated in the period of elongation of mandibles during soldier development. In situ hybridization showed that HsjInR was strongly expressed in the mandibular epithelial tissues, and RNA interference (RNAi) for HsjInR disrupted soldier-specific morphogenesis including mandibular elongation. These results suggest that signaling through the IIS pathway is required for soldier-specific morphogenesis. In addition, up-regulation of the IIS pathway in other body tissues occurred at earlier stages of development, indicating that there is tissue-specific IIS regulation. Because the IIS pathway is generally thought to act upstream of JH in insects, our results suggest the damp-wood termite may have evolved a novel feedback loop between JH and IIS that enables social interactions, rather than nutrition, to regulate caste determination.

    DOI: 10.1002/jez.b.22501

    Web of Science

    PubMed

  24. Genetic and Neural Bases for Species-Specific Behavior in Drosophila Species. Reviewed

    Yamamoto D, Ishikawa Y

    J Neurogenetics   Vol. 27 ( 3 ) page: 130-142   2013

     More details

    Language:English  

    DOI: 10.3109/01677063.2013.800060

  25. Hidden aggression in termite workers: plastic defensive behaviour dependent upon social context Reviewed

    Yuki Ishikawa, Toru Miura

    ANIMAL BEHAVIOUR   Vol. 83 ( 3 ) page: 737 - 745   2012.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD  

    Social insect colonies are self-organized systems that respond to changes in environmental conditions by altering the relative proportions of certain castes or individuals engaged in specific tasks. While termites are known to regulate the ratio of morphologically specialized soldiers in response to the proportion of nestmate castes, the soldier differentiation process requires a relatively long time to be accomplished. Consequently, termites are thought to employ other plastic and flexible mechanisms for colony defence. We quantified the defensive behaviours of soldiers, pseudergates (workers) and reproductives (neotenics) of the damp-wood termite Hodotermopsis sjostedti. When individuals of the caste were exposed to an intruder, soldiers exhibited the most vigorous defensive behaviour, followed by pseudergates and then reproductives. While the aggression levels of soldiers and reproductives were independent of the accompanying castes, pseudergate aggression was more plastic and depended on the other castes present. When paired with reproductives, pseudergates exhibited high levels of aggression towards enemies. However, pseudergate aggression levels remained low when they were paired with soldiers, suggesting that pseudergates moderated their defensive behaviour depending on social context. Plasticity with respect to social behaviours may facilitate rapid and flexible responses required for colony defence. (C) 2011 The Association for the Study of Animal Behaviour. Published by Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.anbehav.2011.12.022

    Web of Science

  26. Gene expression changes during caste-specific neuronal development in the damp-wood termite Hodotermopsis sjostedti. Reviewed International journal

    Yuki Ishikawa, Yasukazu Okada, Asano Ishikawa, Hitoshi Miyakawa, Shigeyuki Koshikawa, Toru Miura

    BMC genomics   Vol. 11 ( 314 ) page: 314 - 314   2010.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BMC  

    BACKGROUND: One of the key characters of social insects is the division of labor, in which different tasks are allocated to various castes. In termites, one of the representative groups of social insects, morphological differences as well as behavioral differences can be recognized among castes. However, very little is known about the neuronal and molecular bases of caste differentiation and caste-specific behavior. In almost all termite species, soldiers play defensive roles in their colonies, and their morphology and behavior are largely different from workers (or pseudergates). Therefore, we predicted that some genes linked to defensive behavior and/or those required for neuronal changes are differentially expressed between workers and soldiers, or during the soldier differentiation, respectively. RESULTS: Using the brain and suboesophageal ganglion (SOG) of the damp-wood termite Hodotermopsis sjostedti, we first screened genes specifically expressed in soldiers or during soldier differentiation by the differential display method, followed by quantitative real-time polymerase chain reaction. No distinctive differences in expression patterns were detected between pseudergates and soldiers. In the course of soldier differentiation, however, five genes were found to be up-regulated in brain and/or SOG: 14-3-3 epsilon, fibrillin2, beta-tubulin, ciboulot, and a hypothetical protein containing a SAP motif. Some of these genes are thought to be associated with cytoskeletal structure or motor-associated proteins in neuronal tissues. CONCLUSION: The identified five genes could be involved in soldier-specific neuronal modifications, resulting in defensive behaviors in termite soldiers. The temporal expression patterns of these genes were consistent with the neuronal changes during soldier differentiation, suggesting that molecular machineries, in which the identified factors would participate, play important roles in behavioral differentiation of termite soldiers.

    DOI: 10.1186/1471-2164-11-314

    Web of Science

    PubMed

  27. Gene up-regulation in response to predator kairomones in the water flea, Daphnia pulex Reviewed

    Hitoshi Miyakawa, Maki Imai, Naoki Sugimoto, Yuki Ishikawa, Asano Ishikawa, Hidehiko Ishigaki, Yasukazu Okada, Satoshi Miyazaki, Shigeyuki Koshikawa, Richard Cornette, Toru Miura

    BMC DEVELOPMENTAL BIOLOGY   Vol. 10 ( 45 ) page: 45   2010.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BMC  

    Background: Numerous cases of predator-induced polyphenisms, in which alternate phenotypes are produced in response to extrinsic stimuli, have been reported in aquatic taxa to date. The genus Daphnia (Branchiopoda, Cladocera) provides a model experimental system for the study of the developmental mechanisms and evolutionary processes associated with predator-induced polyphenisms. In D. pulex, juveniles form neckteeth in response to predatory kairomones released by Chaoborus larvae (Insecta, Diptera).Results: Previous studies suggest that the timing of the sensitivity to kairomones in D. pulex can generally be divided into the embryonic and postembryonic developmental periods. We therefore examined which of the genes in the embryonic and first-instar juvenile stages exhibit different expression levels in the presence or absence of predator kairomones. Employing a candidate gene approach and identifying differentially-expressed genes revealed that the morphogenetic factors, Hox3, extradenticle and escargot, were up-regulated by kairomones in the postembryonic stage and may potentially be responsible for defense morph formation. In addition, the juvenile hormone pathway genes, JHAMT and Met, and the insulin signaling pathway genes, InR and IRS-1, were up-regulated in the first-instar stage. It is well known that these hormonal pathways are involved in physiological regulation following morphogenesis in many insect species. During the embryonic stage when morphotypes were determined, one of the novel genes identified by differential display was up-regulated, suggesting that this gene may be related to morphotype determination. Biological functions of the up-regulated genes are discussed in the context of defense morph formation.Conclusions: It is suggested that, following the reception of kairomone signals, the identified genes are involved in a series of defensive phenotypic alterations and the production of a defensive phenotype.

    DOI: 10.1186/1471-213X-10-45

    Web of Science

    PubMed

  28. A mathematical model for caste differentiation in termite colonies (Isoptera) by hormonal and pheromonal regulations Reviewed

    Ikemoto Y, Ishikawa Y, Miura T, Asama H

    Sociobiology   Vol. 54   page: 841-859   2009

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  29. A Mathematical Model for Caste Differentiation in Termite Colonies (Isoptera) by Hormonal and Pheromonal Regulations

    Ikemoto Yusuke, Ishikawa Yuki, Miura Toru, Asama Hajime

    SOCIOBIOLOGY   Vol. 54 ( 3 ) page: 841 - 859   2009

     More details

  30. A Mathematical Model for Caste Differentiation in Termite Colonies (Isoptera) by Hormonal and Pheromonal Regulations Reviewed

    Yusuke Ikemoto, Yuki Ishikawa, Toru Miura, Hajime Asama

    SOCIOBIOLOGY   Vol. 54 ( 3 ) page: 841 - 859   2009

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:CALIFORNIA STATE UNIV  

    In social insects, the division of labor among castes is one of the fundamental natures for establishing social behaviors. In termites, because of their hemimetaboly, the regulations of postembryonic development should be important to regulate appropriate caste ratios in a colony. Although the caste differentiation can be triggered social Interactions among colony members, the interactions via primer pheromones and the physiological mechanisms underlying caste determination or differentiation are poorly understood. In this study therefore, we propose a model for the regulatory mechanism controlling caste differentiation, focusing oil the physiological states and inhibitory interactions among individuals. The proposed model incorporates an internal physiological system like JH and ecdysone actions, together with two types of inhibitory interactions by pheromones secreted by soldiers and alates. The results Of computer simulations based oil the proposed model suggested that the two types of interactions were sufficient to enable a colony to maintain the appropriate caste ratio. These results Should help to find the primer pheromones and to infer their functions in the caste differentiation in termites.

    Web of Science

  31. Soldier-specific modification of the mandibular motor neurons in termites. Reviewed International journal

    Yuki Ishikawa, Hitoshi Aonuma, Toru Miura

    PloS one   Vol. 3 ( 7 ) page: e2617   2008.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PUBLIC LIBRARY SCIENCE  

    Social insects exhibit a variety of caste-specific behavioral tendencies that constitute the basis of division of labor within the colony. In termites, the soldier caste display distinctive defense behaviors, such as aggressively attacking enemies with well-developed mandibles, while the other castes retreat into the colony without exhibiting any aggressive response. It is thus likely that some form of soldier-specific neuronal modification exists in termites. In this study, the authors compared the brain (cerebral ganglion) and the suboesophageal ganglion (SOG) of soldiers and pseudergates (workers) in the damp-wood termite, Hodotermopsis sjostedti. The size of the SOG was significantly larger in soldiers than in pseudergates, but no difference in brain size was apparent between castes. Furthermore, mandibular nerves were thicker in soldiers than in pseudergates. Retrograde staining revealed that the somata sizes of the mandibular motor neurons (MdMNs) in soldiers were more than twice as large as those of pseudergates. The enlargement of MdMNs was also observed in individuals treated with a juvenile hormone analogue (JHA), indicating that MdMNs become enlarged in response to juvenile hormone (JH) action during soldier differentiation. This enlargement is likely to have two functions: a behavioral function in which soldier termites will be able to defend more effectively through relatively faster and stronger mandibular movements, and a developmental function that associates with the development of soldier-specific mandibular muscle morphogenesis in termite head. The soldier-specific enlargement of mandibular motor neurons was observed in all examined species in five termite families that have different mechanisms of defense, suggesting that such neuronal modification was already present in the common ancestor of termites and is significant for soldier function.

    DOI: 10.1371/journal.pone.0002617

    Web of Science

    PubMed

  32. Differences in mechanosensory hairs among castes of the damp-wood termite Hodotermopsis sjostedti (Isoptera: Termitidae) Reviewed

    Ishikawa Y, Koshikawa S, Miura T

    Sociobiology   Vol. 50   page: 895-907   2007

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  33. Differences in mechanosensory hairs among castes of the damp-wood termite Hodotermopsis sjostedti (Isoptera : Termopsidae)

    Ishikawa Yuki, Koshikawa Shigeyuki, Miura Toru

    SOCIOBIOLOGY   Vol. 50 ( 3 ) page: 895 - 907   2007

     More details

  34. Soldier-specific neural modification in termites

    Ishikawa Yuki, Miura Toru

    NEUROSCIENCE RESEARCH   Vol. 58   page: S231 - S231   2007

     More details

  35. Differences in mechanosensory hairs among castes of the damp-wood termite Hodotermopsis sjostedti (Isoptera: Termitidae). Reviewed

    Ishikawa Y, Koshikawa S, Miura T

    Sociobiology   Vol. 50   page: 895 - 907   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  36. Differences in mechanosensory hairs among castes of the damp-wood termite Hodotermopsis sjostedti (Isoptera : Termopsidae) Reviewed

    Yuki Ishikawa, Shigeyuki Koshikawa, Toru Miura

    SOCIOBIOLOGY   Vol. 50 ( 3 ) page: 895 - 907   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:CALIF STATE UNIV  

    Termites are one of the major groups of social insects, which comprise alates, workers (pseudergates), and soldiers within a species. These castes have different roles and behaviors, and undertake division of labor to increase the inclusive fitness of their colony. On the basis of the different behavioral repertoires, caste-specific neural modifications are predicted, such as modification of sensory systems, i.e., inputs into the nervous system. This study evaluated these sensory-system differences based on mechanoreceptive sensilla length among castes of the damp-wood termite Hodotermopsis sjostedti. We found that soldiers and alates had longer sensilla than pseudergates, and that this variation among castes differed with body region. Specifically, the differences were particularly conspicuous on head capsules and pronota, while sensilla on mouthparts and legs were of similar lengths among castes. It is proposed that soldiers and alates use these long mechanoreceptive structures to sense faint vibrations, an important: capability for their defensive role in detecting enemies and cracks in nest wood.

    Web of Science

▼display all

Books 6

  1. 生き物と音の事典

    石川 由希, 上川内 あづさ( Role: Contributor ,  ショウジョウバエの音コミュニケーション)

    朝倉書店  2019.11  ( ISBN:9784254171679

  2. 遺伝子から解き明かす脳の不思議な世界

    上川内 あづさ, 石川 由希( Role: Contributor ,  第4章 小型でハイスペックな脳の獲得 |昆虫の脳)

    一色出版  2018.12  ( ISBN:9784909383051

     More details

    Total pages:520   Responsible for pages:129-168   Language:Japanese Book type:Scholarly book

  3. Hearing in Drosophila, In: Insect Hearing

    Azusa Kamikouchi, Yuki Ishikawa( Role: Joint author)

    Springer Japan  2016 

  4. シロアリの事典

    吉村 剛, 板倉修司, 岩田隆太郎, 大村和香子, 杉尾幸司, 竹松葉子, 徳田 岳, 松浦健二, 三浦 徹, 玉本 奈々

    海青社  2012.12  ( ISBN:4860992601

     More details

    Total pages:472  

    ASIN

  5. 研究者が教える動物飼育 第2巻 -昆虫とクモの仲間-

    針山 孝彦, 小柳 光正, 嬉 正勝, 妹尾 圭司, 小泉 修, 日本比較生理生化学会

    共立出版  2012.5  ( ISBN:4320057198

     More details

    Total pages:224  

    ASIN

  6. 行動遺伝学入門: 動物とヒトの“こころ”の科学

    小出 剛, 山元 大輔

    裳華房  2011.11  ( ISBN:4785358475

     More details

    Total pages:217  

    ASIN

▼display all

MISC 9

  1. Neuronal mechanisms of evolution of species-specific pheromone preference in Drosophila

    Yuki Ishikawa, Azusa Kamikouchi, Daisuke Yamamoto

    GENES & GENETIC SYSTEMS   Vol. 91 ( 6 ) page: 325 - 325   2016.12

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:GENETICS SOC JAPAN  

    Web of Science

  2. シロアリからショウジョウバエへ : 行動進化メカニズムの理解を目指して

    石川 由希

    比較生理生化学   Vol. 30 ( 2 ) page: 77 - 78   2013.5

     More details

    Language:Japanese  

    CiNii Books

  3. 兵隊シロアリの攻撃性はチラミンによってもたらされる

    石川由希, 青沼仁志, 佐々木謙, 三浦徹

    日本動物学会大会予稿集   Vol. 82nd   page: 144   2011.8

     More details

    Language:Japanese  

    J-GLOBAL

  4. オオシロアリの腹板腺におけるカースト間差異の解析

    淀井智也, 宮川一志, 石川由希, 北條賢, 三浦徹

    日本生態学会大会講演要旨集   Vol. 58th   page: 500   2011.3

     More details

    Language:Japanese  

    J-GLOBAL

  5. Sociogenomics in Termites: Caste Development and Underlying Developmental and Physiological Systems

    Miura T, Cornette R, Ishikawa Y, Hattori A, Gotoh H, Hayashi Y, Koshikawa S

    20th CDB Meeting Kobe     2011.2

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

  6. シロアリの兵隊‐ワーカーにおける脳内チラミン‐オクトパミン系の解析

    石川由希, 青沼仁志, 佐々木謙, 三浦徹

    日本比較生理生化学会大会予稿集   Vol. 31st   page: 71   2009

     More details

    Language:Japanese  

    J-GLOBAL

  7. シロアリのカースト分化と社会行動の分子基盤

    MIURA TOORU, ISHIKAWA YUKI, CORNETTE RICHARD, KOSHIKAWA SHIGEYUKI

    日本動物学会大会要旨集   Vol. 78th   page: 122   2007.8

     More details

    Language:Japanese   Publishing type:Research paper, summary (national, other academic conference)  

    J-GLOBAL

  8. Soldier-specific neural modification in termites

    Yuki Ishikawa, Toru Miura

    NEUROSCIENCE RESEARCH   Vol. 58   page: S231 - S231   2007

     More details

    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ELSEVIER IRELAND LTD  

    Web of Science

  9. シロアリのカースト分化機構はどこまでわかったか

    MIURA TOORU, KOSHIKAWA SHIGEYUKI, CORNETTE RICHARD, FUJITA AI, ISHIKAWA YUKI, HOJO YU, MAEKAWA KIYOTO, MATSUMOTO TADAO

    日本生態学会大会講演要旨集   Vol. 53rd   page: 207   2006.3

     More details

    Language:Japanese   Publishing type:Research paper, summary (national, other academic conference)  

    J-GLOBAL

▼display all

Presentations 31

  1. カザリショウジョウバエはどのように花を選ぶのか?

    石川 由希, 藤井 航平, 林 優人, 武方 宏樹, 田中 良弥, 上川内 あづさ

    第69回日本生態学会大会  2022.3.14 

     More details

    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

  2. カザリショウジョウバエの訪花行動と関連する感覚やシグナルの検討 Invited

    石川由希

    ショウジョウバエ多様性研究会  2021.11.8 

     More details

    Event date: 2021.11 - 2121.11

    Language:Japanese   Presentation type:Oral presentation (general)  

  3. <i>Drosophila elegans</i> as a new model for understanding the neural mechanism of flower recognition

    Yuki Ishikawa, Kohei Fujii, Yuto Hayashi, Hiroki Takekata, Ryoya Tanaka, Azusa Kamikouchi

    14th Japan Drosophila Research Conference (JDRC14), Tsukuba  2021.9.14 

     More details

    Event date: 2021.9

    Language:English   Presentation type:Oral presentation (general)  

  4. カザリショウジョウバエの訪花選好性と、訪花を受ける花の性質の(非)共通性

    石川 由希, 藤井 航平, 林 優人, 武方 宏樹, 田中 良弥, 上川内 あづさ

    日本進化学会第23回東京大会  2021.8.19 

     More details

    Event date: 2021.8

    Language:Japanese   Presentation type:Poster presentation  

  5. 花に棲むカザリショウジョウバエの訪花選好性

    石川 由希, 林 優人, 藤井 航平, 武方 宏樹, 田中 良弥, 上川内 あづさ

    日本生態学会第68回全国大会  2021.3.19 

     More details

    Event date: 2021.3

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

  6. Song exposure modifies the male courtship strategy to chase females in Drosophila melanogaster

    Azusa Kamikouchi, Yuki Ishikawa, Takuya Maekawa, Daisuke Takaichi

    JSCPB 2020 Yamagata  2020.11.23 

     More details

    Event date: 2020.11

    Language:English  

  7. Flower-visiting behavior of Drosophila elegans in Okinawa

    Yuki Ishikawa, Yuto Hayashi, Kohei Fujii, Hiroki Takekata, Ryoya Tanaka, Azusa Kamikouchi

    JSCPB 2020 Yamagata  2020.11.23 

     More details

    Event date: 2020.11

    Language:English   Presentation type:Oral presentation (general)  

  8. Identifying auditory neurons that shape song selectivity in two Drosophila species

    Takuro Ohashi, Yuki Ishikawa, Takeshi Awasaki, Su Matthew, Azusa Kamikouchi

    JSCPB 2020 Yamagata  2020.11.22 

     More details

    Event date: 2020.11

    Language:English   Presentation type:Oral presentation (general)  

  9. Host selectivity of the flower-feeding fruit fly Drosophila elegans

    2020.9.9 

     More details

    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

  10. 花に棲むショウジョウバエの訪花選好性とテリトリー行動 Invited

    石川由希

    デジタル進化生物セミナー  2020.8.7 

     More details

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  11. Ecology, genome editing, and genetic tools in the flower-dependent fruit fly Drosophila elegans Invited

    2021.12.3 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  12. A neural mechanism underlying rapid evolution of mating preference Invited International conference

    Yuki Ishikawa

    Japanese-German Frontiers of Science Symposium  2018.9.6 

     More details

    Language:English   Presentation type:Poster presentation  

  13. 配偶者選好性の進化をもたらす神経基盤 Invited

    石川 由希

    生命科学の最前線-独自の視点から挑む多彩な生命現象を体感せよ-  2017.6.13 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  14. 近縁種との交配を抑制するフェロモン情報処理の進化

    石川 由希

    昆虫脳コネクトーム研究若手研究発表会  2018.12.4 

     More details

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  15. 種特異的なフェロモン選好性の進化をもたらす神経基盤

    石川 由希

    第65回日本生態学会  2018.3.15 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

  16. 性フェロモン選好性の進化的逆転をもたらす神経基盤

    石川 由希, 前田 直希, 上川内 あづさ

    日本進化学会第21回大会  2019.8.7 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

  17. 性フェロモンへの好みを決める神経回路の進化 Invited

    石川 由希

    蚕糸・昆虫機能利用学術講演会  2019.3.22 

     More details

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  18. Neural basis underlying evolution of pheromone preference in fruit fly

    Yuki Ishikawa, Naoki Maeda, Azusa Kamikouchi

    The 41st Annual Meeting of the Japan Neuroscience Society  2018.7.26 

     More details

    Language:English   Presentation type:Poster presentation  

  19. フェロモン選好性の進化を実現する神経回路の変化

    Yuki Ishikawa, Naoki Maeda, Azusa Kamikouchi

    20th Annual Meeting of the Society of Evolutionary Studies, Japan  2018.8.22 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

  20. ショウジョウバエの種間交雑を妨げるフェロモン選好性の進化の神経基盤

    石川 由希

    Evo-Devo青年の会  2017.6.16 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

  21. ショウジョウバエの求愛歌選好性における経験依存的な変化とその神経基盤 Invited

    石川 由希

    第38回日本動物行動学会大会  2019.11.22 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  22. ショウジョウバエのフェロモン選好性の進化をもたらす神経基盤

    石川 由希

    日本進化学会第19回目  2017.8.24 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

  23. ショウジョウバエにおける化学コミュニケーションに寄与する神経回路の進化

    石川 由希

    第42回日本神経科学学会  2019.7.26 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  24. How does fly discriminates conspecific song? Invited

    Yuki Ishikawa

    The 78th Annual Meeting of the Japanese Society for Animal Psychology  2018.8.28 

     More details

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  25. アサガオの花に棲むカザリショウジョウバエのテリトリー認識機構

    石川 由希

    第67回日本生態学会大会  2020.3.6 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

  26. The neural mechanism underlying evolution of mating preference International conference

    Yuki Ishikawa, Naoki Maeda, Kaori Mochizuki, Gakutami Nomura, Azusa Kamikouchi, Daisuke Yamamoto

    The 46th Naito Conference: Mechanisms of Evolution and Biodiversity  2018.10.2 

     More details

    Language:English   Presentation type:Poster presentation  

  27. Neural mechanisms underlying the auditory information processing in the fruit fly Invited

    Yuki ISHIKAWA, Daichi YAMADA, Natsuki OKAMOTO, Mizuki NAKAMURA, Hyunsoo KIM, Azusa KAMIKOUCHI

    日本比較生理生化学会第39回大会  2017.11.23 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  28. Neural mechanism underlying rapid evolution of sex-pheromone preference

    Yuki Ishikawa, Naoki Maeda, Azusa Kamikouchi

    The 13th Japanese Drosophila Research Conference  2018.9.10 

     More details

    Language:English   Presentation type:Poster presentation  

  29. Neural mechanism underlying evolution of mating preference in fruit fly Invited International conference

    Yuki Ishikawa

    ICOB&NPAS Joint Seminar  2018.8.13 

     More details

    Language:English   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  30. Neural basis underlying evolution of mating preference Invited International conference

    Yuki Ishikawa

    Neuro Grobal Focusd symposium "Sex on the brain"  2018.2.20 

     More details

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  31. Evolutionary change in a neural network underlying mating preference of fruit fly Invited

    Yuki Ishikawa

    2018.11.16 

     More details

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

▼display all

KAKENHI (Grants-in-Aid for Scientific Research) 16

  1. Regulatory mechanism of target recognition with multi-sensory system in a small brain

    2021.9 - 2025.3

    JST 

      More details

    Authorship:Principal investigator 

  2. 神経ネットワークにおける揺らぎと進化的保存性の関係

    Grant number:20H04865  2020.4 - 2022.3

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    石川 由希

      More details

    Authorship:Principal investigator 

    Grant amount:\10270000 ( Direct Cost: \7900000 、 Indirect Cost:\2370000 )

    配偶相手を選ぶ「好み」の分化は、生物が種分化する過程で重要なステップである。一方、ひとつの種の中でも、個体によって好みはばらつく。このような種内レベルのばらつきから、どのように好みが種間で分化するかは、生物の進化を理解する上で重要な命題である。配偶相手に対する好みは、脳内の神経ネットワークに規定されている。動物の神経ネットワークは経験やゲノム変異によって揺らぎ、好みのばらつきを生む。では、この揺らぎからどのように種間の分化が生じるのだろうか?また揺らぎが分化に果たす役割はあるのだろうか?本研究では、配偶相手への好みの異なる2種のショウジョウバエを用いてこの問いに答える。

  3. シナプス接続の獲得/喪失による生殖隔離の進化とその分子基盤

    Grant number:19K22453  2019.6 - 2022.3

    日本学術振興会  科学研究費助成事業 挑戦的研究(萌芽)  挑戦的研究(萌芽)

    石川 由希

      More details

    Authorship:Principal investigator 

    Grant amount:\6370000 ( Direct Cost: \4900000 、 Indirect Cost:\1470000 )

    行動的な生殖隔離の獲得は、種分化や性選択を介してさらなる形質進化を駆動する。本研究は、フェロモン選好性の変化によって生殖隔離を実現したキイロショウジョウバエとオナジショウジョウバエをモデルに、この選好性の進化をもたらした情報処理プロセスの特定を目指す。
    本研究の提案する「シナプス獲得/喪失仮説」を検証するために、遺伝学的ツールの整備されていないオナジショウジョウバエと同様のフェロモン選好性を持つF1雑種をオナジ型フェロモン選好性のモデルとして用いた。当該年度は、フェロモン特徴抽出回路を構成する7種のニューロン群間のシナプス接続をGRASP法を用いてキイロショウジョウバエと雑種で比較した。その結果、特定の神経接続のみがF1雑種において失われていることが示唆された。一方近年、GRASP法には、神経接続以外でGFPが再構成される偽陽性が報告されている。今回特定した神経接続の差異が偽陽性に起因するものではないことを確かめるため、特定の神経接続のシナプス前細胞とシナプス後細胞を標識し、観察するtwo-TagEM法を用いた。当該年度は、本研究で特定した神経接続のシナプス前細胞のミトコンドリアとシナプス後細胞の細胞膜にペルオキシダーゼを発現させる系統を確立し、これらを同時に標識観察できる条件を検討した。様々な条件を検討した結果、これら2種類の細胞を同時に標識できる条件を確立することができた。今後はこの条件を用いて特定した神経接続を透過電子顕微鏡レベルで観察することを目指す。

  4. シナプス接続の獲得/喪失による生殖隔離の進化とその分子基盤

    2019.4 - 2021.3

    挑戦的研究(萌芽) 

    石川 由希

      More details

    Authorship:Principal investigator  Grant type:Competitive

  5. Neural basis for evolution of mating preference

    Grant number:18H02488  2018.4 - 2021.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Ishikawa Yuki

      More details

    Authorship:Principal investigator 

    Grant amount:\17420000 ( Direct Cost: \13400000 、 Indirect Cost:\4020000 )

    To unravel the neural basis of the evolution of mate preference, we aimed to elucidate the neural basis of the interspecific differences in the preference for courtship songs in Drosophila melanogaster and D. simulans. We found that the morphological characteristics of the 1st, 2nd, and 3rd order neurons and courtship command neurons are well conserved between the two species. We also compared the song preference of the neural activity of auditory 1st and 2nd order neurons between species and showed that there are slight interspecific differences in the neural response of 2nd order neurons. The results show that the interspecies differences in auditory information processing already exist at the level of neural activity of second-order neurons.

  6. 神経ネットワークの揺らぎは配偶者選好性の進化を規定しうるか

    Grant number:18H04819  2018.4 - 2020.3

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    石川 由希

      More details

    Authorship:Principal investigator 

    Grant amount:\11180000 ( Direct Cost: \8600000 、 Indirect Cost:\2580000 )

    本研究は、ショウジョウバエのフェロモン選好性を規定する神経ネットワークに着目し、この揺らぎやすさがどのように選好性の進化を規定しうるのかを明らかにすることを目的とする。キイロショウジョウバエのフェロモン選好ネットワークは、メス特有のフェロモン7,11-HDの情報を受け取り、求愛を制御することが知られる。そこで私はこのフェロモン選好ネットワークに着目し、その揺らぎやすさとフェロモン選好性の関係を明らかにしようとした。
    当該年度は、神経接続を可視化する再構成GFP法を用いてた神経接続の比較を、フェロモン選好ネットワークを構成するニューロン群全てに適用した。その結果、雑種において、特定の神経接続のみが失われており、それ以外の経路の神経接続は全て保存されていることが示唆された。これまで知られているフェロモン選好ネットワークの回路構造を併せて考慮すると、今回特定された神経接続の喪失がフェロモン選好ネットワークの機能を転換させる可能性が考えられる。
    再構成GFP法には、神経接続がなくてもGFPシグナルが検出される偽陽性が報告された。今回の結果が偽陽性に依るものではないことを確かめるため、透過電子顕微鏡レベルで特定の神経接続を観察できるtwo-TagEM法を今回特定した神経接続に適用した。当該年度はまず、シナプス前細胞のミトコンドリアとシナプス後細胞の細胞膜を透過電子顕微鏡により同時に標識観察できる条件を検討した。様々な染色条件を試した結果、シナプス前細胞のミトコンドリアと、シナプス後細胞の細胞膜を同時にDAB標識できる条件を特定できた。今後はこの条件を用いて、当該神経接続の観察を目指す。

  7. 神経ネットワークの揺らぎは配偶者選好性の進化を規定しうるか

    2018.4 - 2020.3

    文科省  新学術領域研究 

    石川 由希

      More details

    Authorship:Principal investigator  Grant type:Competitive

  8. フェロモン選好性の進化的逆転をもたらす神経回路の変化

    2018.4 - 2019.3

    成茂基金  公益信託 成茂動物科学振興基金 

    石川 由希

      More details

    Authorship:Principal investigator  Grant type:Competitive

  9. Evolutionary origin of the neural mechanisms underlying sexual isolation of Drosophila

    Grant number:17K19425  2017.6 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)  Grant-in-Aid for Challenging Research (Exploratory)

    Ishikawa Yuki, Kamikouchi Azusa

      More details

    Authorship:Principal investigator 

    Grant amount:\6370000 ( Direct Cost: \4900000 、 Indirect Cost:\1470000 )

    Animals often avoid to mate with heterospecific individuals. To understand the neural mechanism underlying the evolution of this behavioral sexual isolation, we focused on Drosophila species, whose sexual isolation mostly depends to cuticular pheromone. First, we screened and identified the sensory neurons which detect heterospecific pheromone and decreases courtship activity. Our further observation of the downstream neurons of these neurons suggests that the acquisition of neural connection between the sensory neurons and the inhibitory downstream neurons contributes the evolution of sexual isolation via heterospecific pheromones.

  10. 同種か異種かを識別する種間認識システムの進化を司る神経分子基盤

    2014.4

    科学研究費補助金  若手研究(B)

      More details

    Authorship:Principal investigator 

  11. Neural mechanisms underlying the evolution of mating preference

    Grant number:26870264  2014.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Ishikawa Yuki, KAMIKOUCHI Azusa

      More details

    Authorship:Principal investigator 

    Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )

    Species-specific mating preference plays important role for the generation and maintenance of biodiversity. However, what kind of change in neural circuits causes the evolution of the preference is largely unknown. To solve this question, I searched the difference of neural circuit causing the species difference of pheromone and song preference in Drosophila. As a result, I found several differences in the neural circuits, which might be involved in the species difference of these preferences. These findings may provide clues to understand how the mating preference to conspecific individuals has been acquired during evolutionary process.

  12. Grant-in-Aid for Young Scientists (B)

    2014.4 - 2017.3

    MEXT  Neural mechanisms underlying the evolution of mating preference 

    Yuki Ishikawa

      More details

    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )

  13. 行動の進化をもたらす遺伝子と神経回路の変化を特定する

    2011.4 - 2013.7

    科学研究費補助金 

      More details

    Authorship:Principal investigator 

  14. 行動の進化をもたらす遺伝子と神経回路の変化を特定する

    Grant number:11J03778  2011 - 2013

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    石川 由希

      More details

    本研究ではDrosophila属の求愛行動の進化をもたらす遺伝子と神経回路を特定することを目的とし、特に求愛相手への選好性に関して、キイロショウジョウバエD. melanogasterと近縁種であるオナジショウジョウバエD. simulans、またこれらのF1雑種個体を用いて研究を進めてきた。
    前年度までの研究の結果、F1雑種個体の求愛活性はmelanogsterフェロモン(特に7,11-heptacosadiene, 7,11-HD)によって抑制され、simulansフェロモン(特に7-tricosene, 7-T)によって促進されていることがわかった。本年度は、遺伝的な操作によって体表フェロモンを持たないmelanogaster雌をターゲットとして用いることで、melanogaster, simulans, F1雑種個体のフェロモンに対する濃度依存的な反応を詳細に比較した。その結果、melanogasterではターゲットに塗布された7,11・HDの濃度依存的に求愛活性が上昇していったのに対し、simulansやF1雑種個体では、7,11・HDの濃度依存的に求愛活性が減少していくことがわかった。また、7,11-HDによる求愛活性抑制反応は、F1雑種個体よりもsimulansにおいて高く、さらにsimulans系統間における反応性の強弱は、次世代のF1雑種個体に引き継がれた。これらの結果から、F1雑種個体の求愛活性はsimulansの求愛活性と同様7,11-HDにより抑制されるが、その抑制はsimulansよりも弱いことがわかった。

  15. シロアリの社会行動をもたらす神経改変の分子機構とその進化

    2008.4 - 2010.3

    科学研究費補助金 

      More details

    Authorship:Principal investigator 

  16. シロアリの社会行動をもたらす神経改変の分子機構とその進化

    Grant number:08J01687  2008 - 2010

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    石川 由希

      More details

    シロアリは高度に発達した社会を形成する真社会性昆虫の代表的なグループである。大部分のシロアリ種にはコロニー防衛に特化した兵隊カーストが存在する。彼らはワーカーとほぼ同じ遺伝的背景を持つにも関わらず、非常に高い攻撃性を示す。本研究ではその高い攻撃性をもたらす分子・神経学的メカニズムの解明を目的とする。
    研究代表者は、兵隊の攻撃性を司る候補因子としてオクトパミンと、その前駆体チラミンに注目した。本研究課題(1年目)におけるHPLCを用いた解析により、兵隊の脳や食道下神経節に含有されるチラミンがワーカーよりも有意に多いことが明らかになった。また、本研究課題(2年目)ではシロアリの攻撃性の計測するための行動解析系の立ち上げを行った。そこで本年度はその行動解析系を用い、オクトパミンやチラミンの機能解析を行った。すると、チラミンの人工投与によってワーカーの攻撃性は有意に高くなるが、オクトパミンにはそのような効果は見られなかった。このことから、兵隊シロアリにおける高い攻撃性は、オクトパミンではなく、チラミンによってもたらされていることが強く示唆された。また、抗チラミン抗体を用いた免疫染色により、シロアリの脳・食道下神経節のチラミン免疫反応性ニューロン(チラミンを含有していると予想されるニューロン)の分布や投射部位を観察した。その結果、DUMニューロンや、OA/TA5,7,8と名付けられたニューロンの細胞体が兵隊特異的に肥大していることが明らかになった。これらのニューロンの肥大化が兵隊の高い攻撃性や防衛行動に関与していると示唆される。

▼display all

 

Social Contribution 1

  1. 第108回 生態学最前線 -昆虫の脳から環境問題まで-

    Role(s):Lecturer

    名古屋市立大学 大学院システム自然科学研究科  サイエンスカフェin名古屋  2016.4

     More details

    Audience: Junior students, High school students, College students, Graduate students, General

    Type:Lecture

    中区栄ナディアパーク7階 7th Cafeにおいて、『生態学最前線』と題してさまざまな分野と融合しつつある生態学の最前線に関する話題を提供した。名古屋市大の村瀬香准教授と共演。