Updated on 2021/11/10

写真a

 
ARAKI Yoshio
 
Organization
Nagoya University Hospital Neurosurgery Lecturer
Graduate School
Graduate School of Medicine
Title
Lecturer

Degree 1

  1. 医学博士 ( 2013.3   名古屋大学 ) 

Current Research Project and SDGs 1

  1. moyamoya disease

Research History 3

  1. 名古屋大学大学院医学系研究科脳神経外科助教

    2015.4 - 2016.12

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    Country:Japan

  2. 名古屋大学医学部付属病院脳神経外科病院助教

    2013.4 - 2015.3

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    Country:Japan

  3. 京都大学医学部付属病院脳神経外科特定病院助教

    2011.11 - 2013.3

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    Country:Japan

Education 1

  1. Nagoya University   Graduate School, Division of Medical Sciences

    - 2011.3

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    Country: Japan

Professional Memberships 2

  1. 神経内視鏡学会   技術認定医

  2. 日本脳神経外科学会   専門医・指導医

 

Papers 49

  1. Advantages of petrosectomy for superior temporal artery to superior cerebellar artery bypass based on three-dimensional distance measurements using cadaver heads Invited Reviewed

    Kenji Uda, Kuniaki Tanahashi, Takashi Mamiya, Fumiaki Kanamori, Kinya Yokoyama, Masahiro Nishihori, Takashi Izumi, Yoshio Araki, Ryuta Saito

    Neurosurg Rev     2021.11

  2. Challenging direct bypass surgery for very young children with moyamoya disease: technical notes Invited Reviewed

    Yoshio Araki, Kenji Uda, Kinya Yokoyama, Fumiaki Kanamori, Michihiro Kurimoto, Yoshiki Shiba, Takashi Mamiya, Masahiro Nishihori, Kazuhito Takeuchi, Kuniaki Tanahashi, Yuichi Nagata, Yusuke Nishimura, Sho Okamoto, Masaki Sumitomo, Takashi Izumi & Ryuta Saito

    Technical Note     2021.10

  3. Outcomes and Issues of ‘Drip and Go’ as an Inter-Hospital Cooperation System in Mechanical Thrombectomy for Acute Ischemic Stroke Invited Reviewed

      Vol. 15 ( 10 ) page: 629 - 636   2021.10

  4. Evaluation of the differences in pressure applied to the vessel wall by different types of balloon remodeling microcatheters in an experimental model Invited Reviewed

    Mizuka Ikezawa, Takashi Izumi, Masahiro Nishihori, Tetsuya Tsukada, Yosuke Tamari, Yoshio Araki, Kinya Yokoyama, Kenji Uda, Shunsaku Goto, Asuka E Kropp, Takafumi Otsuka, Naoki Kato, Mizuki Nakano, Ryuta Saito

    Interv Neuroradiol     2021.9

  5. Genetic and nongenetic factors for contralateral progression of unilateral moyamoya disease: the first report from the SUPRA Japan Study Group Invited Reviewed

    Yohei Mineharu MD, PhD1, Yasushi Takagi MD, PhD2, Akio Koizumi MD, PhD3, Takaaki Morimoto MD, PhD4, Takeshi Funaki MD, PhD1, Tomohito Hishikawa MD, PhD5, Yoshio Araki MD, PhD6, Hitoshi Hasegawa MD, PhD7, Jun C. Takahashi MD, PhD8, Satoshi Kuroda MD, PhD9, Kiyohiro Houkin MD, PhD10, and Susumu Miyamoto MD, PhD1

    Journal of Neurosurgery     2021.9

  6. Transcriptome-wide analysis of intracranial artery in patients with moyamoya disease showing upregulation of immune response, and downregulation of oxidative phosphorylation and DNA repair Invited Reviewed

    Fumiaki Kanamori, Kinya Yokoyama, Akinobu Ota, Kazuhiro Yoshikawa, Sivasundaram Karnan, Mikio Maruwaka, Kenzo Shimizu, Shinji Ota, Kenji Uda, Yoshio Araki, Sho Okamoto, Satoshi Maesawa, Toshihiko Wakabayashi, Atsushi Natsume

    Neurosurg Focus .   Vol. 51   page: E3   2021.9

  7. Coexistence of a Dural Arteriovenous Fistula and Pial Arteriovenous Malformation Sharing a Common Drainer Invited Reviewed

    Kenji UDA, Takashi IZUMI, Fumiaki KANAMORI, Kinya YOKOYAMA, Tetsuya TSUKADA, Masahiro NISHIHORI, Kazunori SHINTAI, Sho OKAMOTO, Yoshio ARAKI

    NMC Case Report Journal   Vol. 8 ( 1 ) page: 557 - 563   2021.8

  8. Streak Metal Artifact Reduction Technique in Cone Beam Computed Tomography Images after Endovascular Neurosurgery Invited Reviewed

    Takafumi OTSUKA, Masahiro NISHIHORI, Takashi IZUMI, Takeshi UEMURA, Takashi SAKAI, Mizuki NAKANO, Naoki KATO, Fumiaki KANAMORI, Tetsuya TSUKADA, Kenji UDA, Kinya YOKOYAMA, Yoshio ARAKI, Ryuta SAITO

    Neurologia medico-chirurgica   Vol. 61 ( 8 ) page: 468 - 474   2021.8

  9. Indocyanine Green Emission Timing of the Recipient Artery in Revascularization Surgery for Moyamoya Disease Invited Reviewed

    Fumiaki Kanamori, Yoshio Araki, Kinya Yokoyama, Kenji Uda, Takashi Mamiya, Masahiro Nishihori, Takashi Izumi, Sho Okamoto, Atsushi Natsume

    Nagoya J Med Sci   Vol. 83 ( 3 ) page: 523 - 534   2021.8

  10. Triggering of Carotid Sinus Reflex during Deployment of the Flow-diverter Device Invited Reviewed

    Shunsaku Goto, Takashi Izumi, Masahiro Nishihori, Tetsuya Tsukada, Yoshio Araki, Kenji Uda, Kinya Yokoyama, Ryuta Saito

    Neurol Med Chir (Tokyo)   Vol. 61 ( 10 ) page: 583 - 590   2021.7

  11. Evaluation of the Straightening Phenomenon of Various Types of Coils Invited Reviewed

    Mamoru Ishida Takashi Izumi Yoshio Araki Masahiro Nishihori Kinya Yokoyama Kenji Uda Tetsuya Tsukada Toshihiko Wakabayashi

    Neurol Med Chir (Tokyo)   Vol. 61 ( 6 ) page: 356 - 360   2021.6

  12. Changes in Vessel Wall Enhancement Related to the Recent Neurological Symptoms in Patients with Moyamoya Disease Invited Reviewed

    Shinsuke MURAOKA,Toshiaki TAOKA, Hisashi KAWAI, Sho OKAMOTO,Kenji UDA, Shinji NAGANAWA, and Yoshio ARAKI     2021.6

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  13. 破裂大型脳底動脈瘤に対するステント支援コイル塞栓術後に2 度の帝王切開による分娩を経験した1例 Invited Reviewed

    加藤 直毅, 泉 孝嗣, 西堀 正洋, 塚田 哲也, 荒木 芳生, 横山 欣也, 宇田 憲司, 松原 功明, 纐纈 直樹

    脳血管内治療   Vol. 6 ( 1 ) page: 24 - 29   2021.5

  14. Levodopa-responsive Parkinsonism Caused by Recurrence of Large Basilar-tip Aneurysm after Stent-assisted Coil Embolization: A Case Report Invited Reviewed

    NMC Case Report Journal   Vol. 8 ( 1 ) page: 107 - 111   2021.4

  15. hort-segment Internal Trapping for Symptomatic Thrombosed Large Fusiform Vertebral Artery Aneurysms (Bird’s Nest Trapping): A Technical Note Invited Reviewed

    Masahiro NISHIHORI, Takashi IZUMI, Tetsuya TSUKADA, Asuka Elisabeth KROPP, Kenji UDA, Kinya YOKOYAMA, Yoshio ARAKI, Toshihiko WAKABAYASHI

    Neurologia medico-chirurgica   Vol. 61 ( 4 ) page: 28 - 291   2021.4

  16. Efficacy of Respiratory Control under Local Anesthesia during Endovascular Therapy in the Tortuous Vertebral Artery with the Use of Respiratory Dislocation of the Aortic Arch Invited Reviewed

    Shunsaku Goto, Takashi Izumi, Masahiro Nishihori, Tetsuya Tsukada, Yoshio Araki, Kinya Yokoyama, Kenji Uda, Mizuka Ikezawa

    Journal of Neuroendovascular Therapy   Vol. 15 ( 3 ) page: 195 - 199   2021.3

  17. Ipsilateral late stroke after revascularization surgery for patients with Moyamoya disease

    Araki Yoshio, Yokoyama Kinya, Uda Kenji, Kanamori Fumiaki, Mamiya Takashi, Nishihori Masahiro, Sumitomo Masaki, Okamoto Sho, Izumi Takashi

    ACTA NEUROCHIRURGICA     2021.2

  18. Analysis of relationship between superior hypophyseal artery visualization and preservation and postoperative visual field deficit in paraclinoid aneurysm Invited Reviewed

    Masato Otawa 1, Takashi Izumi 1, Masahiro Nishihori, Tetsuya Tsukada, Ryosuke Oshima, Tomomi Kawaguchi, Shunsaku Goto, Mizuka Ikezawa, Asuka Elisabeth Kropp, Yoshio Araki, Kenji Uda, Toshihiko Wakabayashi

    Nagoya J Med Sci .   Vol. 83 ( 1 ) page: 21 - 30   2021.2

  19. Postoperative stroke and neurological outcomes in the early phase after revascularization surgeries for moyamoya disease: an age-stratified comparative analysis

    Araki Yoshio, Yokoyama Kinya, Uda Kenji, Kanamori Fumiaki, Kurimoto Michihiro, Shiba Yoshiki, Mamiya Takashi, Nishihori Masahiro, Izumi Takashi, Sumitomo Masaki, Okamoto Sho, Matsui Kota, Emoto Ryo, Wakabayashi Toshihiko, Matsui Shigeyuki, Natsume Atsushi

    NEUROSURGICAL REVIEW     2021.1

  20. Effects of aspirin and heparin treatment on perioperative outcomes in patients with Moyamoya disease

    Kanamori Fumiaki, Araki Yoshio, Yokoyama Kinya, Uda Kenji, Mamiya Takashi, Nishihori Masahiro, Izumi Takashi, Okamoto Sho, Natsume Atsushi

    ACTA NEUROCHIRURGICA     2021.1

  21. A case of internal trapping to a thrombosed giant rapidly growing aneurysm at the posterior cerebral artery

    Otawa Masato, Izumi Takashi, Nishihori Masahiro, Tsukada Tetsuya, Oshima Ryosuke, Kawaguchi Tomomi, Goto Shunsaku, Ikezawa Mizuka, Kropp Asuka Elisabeth, Araki Yoshio, Yokoyama Kinya, Uda Kenji, Wakabayashi Toshihiko

    NAGOYA JOURNAL OF MEDICAL SCIENCE   Vol. 82 ( 3 ) page: 557 - 566   2020.8

  22. Surgical Designs of Revascularization for Moyamoya Disease: 15 Years of Experience in a Single Center

    Araki Yoshio, Uda Kenji, Yokoyama Kinya, Kanamori Fumiaki, Mamiya Takashi, Nishihori Masahiro, Izumi Takashi, Tanahashi Kuniaki, Masaki Sumitomo, Sho Okamoto, Wakabayashi Toshihiko, Natsume Atsushi

    WORLD NEUROSURGERY   Vol. 139   page: E325 - E334   2020.7

  23. 小児もやもや病血行再建術後のperiventricular anastomosis Invited Reviewed

    金森 史哲, 荒木 芳生, 横山 欣也, 宇田 憲司, 真宮 崇, 若林 俊彦, 夏目 敦至

    脳神経外科ジャーナル   Vol. 29 ( 6 ) page: 442 - 447   2020.6

  24. 血液凝固第XIII因子濃縮製剤使用後,Galen静脈血栓症が疑われた1例 Invited Reviewed

    中野瑞生、荒木芳生、金森史哲、宇田憲司、真宮崇、若林俊彦、夏目敦至

    脳卒中   Vol. 42 ( 3 ) page: 442 - 447   2020.5

  25. In Reply to the Letter to the Editor Regarding "Brain Compression by Encephalo-Myo-Synangiosis is a Risk Factor for Transient Neurological Deficits After Surgical Revascularization in Pediatric Patients with Moyamoya Disease"

    Kanamori Fumiaki, Araki Yoshio

    WORLD NEUROSURGERY   Vol. 136   page: 443 - 443   2020.4

  26. Brain Compression by Encephalo-Myo-Synangiosis is a Risk Factor for Transient Neurological Deficits After Surgical Revascularization in Pediatric Patients with Moyamoya Disease

    Kanamori Fumiaki, Araki Yoshio, Yokoyama Kinya, Uda Kenji, Nishihori Masahiro, Izumi Takashi, Okamoto Sho, Wakabayashi Toshihiko

    WORLD NEUROSURGERY   Vol. 133   page: E558 - E566   2020.1

  27. Posterior Cerebral Artery Reconstruction by In-Situ Bypass with Superior Cerebellar Artery via Occipital Transtentorial Approach

    Tanahashi Kuniaki, Araki Yoshio, Uda Kenji, Muraoka Shinsuke, Motomura Kazuya, Lushun Chalise, Wakabayashi Toshihiko, Natsume Atsushi

    WORLD NEUROSURGERY   Vol. 126   page: 24 - 29   2019.6

  28. Intraoperative evaluation of local cerebral hemodynamic change by indocyanine green videoangiography: prediction of incidence and duration of postoperative transient neurological events in patients with moyamoya disease.

    Uda K, Araki Y, Muraoka S, Ota S, Wada K, Yokoyama K, Nishihori M, Izumi T, Okamoto S, Wakabayashi T

    Journal of neurosurgery   Vol. 130 ( 4 ) page: 1367 - 1375   2019.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3171/2017.10.JNS171523

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  29. Progressive stenosis and radiological findings of vasculitis over the entire internal carotid artery in moyamoya vasculopathy associated with graves' disease: a case report and review of the literature

    Ito Hiroto, Yokoi Syunsuke, Yokoyama Kinya, Asai Takumi, Uda Kenji, Araki Yoshio, Takasu Syuntaro, Kobayashi Rei, Okada Hisashi, Okuda Satoshi

    BMC NEUROLOGY   Vol. 19   2019.3

  30. もやもや病に対する間接血行再建術後における皮質および 脳溝内の FLAIR 高信号と術後一過性神経脱落症状との関連 Invited Reviewed

    荒木芳生、岡本奨、横山欣也、太田慎次、宇田憲司、村岡真輔、金森史哲、西堀正洋、泉孝嗣、若林俊彦

    脳卒中の外科   Vol. 46 ( 6 ) page: 439 - 444   2018.12

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    Authorship:Lead author  

  31. Subarachnoid hemorrhage induces neuronal nitric oxide synthase phosphorylation at Ser(1412) in the dentate gyrus of the rat brain

    Wada Kentaro, Osuka Koji, Watanabe Yasuo, Usuda Nobuteru, Fukasawa Motoaki, Araki Yoshio, Okamoto Sho, Wakabayashi Toshihiko

    NITRIC OXIDE-BIOLOGY AND CHEMISTRY   Vol. 81   page: 67 - 74   2018.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.niox.2017.10.007

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  32. Plastic hinge relocation in reinforced concrete beams using Cu-Al-Mn SMA bars

    Pareek S., Suzuki Y., Araki Y., Youssef M. A., Meshaly M.

    ENGINEERING STRUCTURES   Vol. 175   page: 765-775   2018.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.engstruct.2018.08.072

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  33. Comparative Prospective Study of Microvascular Anastomosis Training by Self-Learning or with Expert Instruction

    Sakamoto Yusuke, Okamoto Sho, Shimizu Kenzo, Araki Yoshio, Hirakawa Akihiro, Wakabayashi Toshihiko

    WORLD NEUROSURGERY   Vol. 118   page: E818 - E824   2018.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.wneu.2018.07.061

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  34. PREDICTION OF INTRACRANIAL ARTERIAL STENOSIS PROGRESSION IN PATIENTS WITH MOYAMOYA VASCULOPATHY: CONTRAST-ENHANCED HIGH-RESOLUTION MAGNETIC RESONANCE VESSELWALL IMAGING

    Muraoka S., Araki Y., Taoka T., Kawai H., Okamoto S., Uda K., Ota S., Naganawa S., Wakabayashi T.

    INTERNATIONAL JOURNAL OF STROKE   Vol. 13   page: 32-32   2018.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  35. POSTOPERATIVE MANAGEMENT OF PEDIATRIC MOYAMOYA DISEASE

    Muraoka S., Araki Y., Kondo G., Kurimoto M., Uda K., Ota S., Okamoto S., Wakabayashi T.

    INTERNATIONAL JOURNAL OF STROKE   Vol. 13   page: 77-77   2018.10

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    Web of Science

  36. Prediction of Intracranial Arterial Stenosis Progression in Patients with Moyamoya Vasculopathy: Contrast-Enhanced High-Resolution Magnetic Resonance Vessel Wall Imaging

    Muraoka Shinsuke, Araki Yoshio, Taoka Toshiaki, Kawai Hisashi, Okamoto Sho, Uda Kenji, Ota Shinji, Naganawa Shinji, Wakabayashi Toshihiko

    WORLD NEUROSURGERY   Vol. 116   page: E1114 - E1121   2018.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.wneu.2018.05.181

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  37. Association between CYP2C19 genotype and the additional effect of cilostazol to clopidogrel resistance in neuroendovascular therapy.

    Tajima H, Izumi T, Miyachi S, Matsubara N, Ito M, Imai T, Nishihori M, Shintai K, Okamoto S, Araki Y, Kumakura Y, Furukawa-Hibi Y, Yamada K, Wakabayashi T

    Nagoya journal of medical science   Vol. 80 ( 2 ) page: 207-215   2018.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.18999/nagjms.80.2.207

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  38. Postoperative Cerebral Infarction Risk Factors and Postoperative Management of Pediatric Patients with Moyamoya Disease

    Muraoka Shinsuke, Araki Yoshio, Kondo Goro, Kurimoto Michihiro, Shiba Yoshiki, Uda Kenji, Ota Shinji, Okamoto Sho, Wakabayashi Toshihiko

    WORLD NEUROSURGERY   Vol. 113   page: E190 - E199   2018.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.wneu.2018.01.212

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  39. Case of Subarachnoid Hemorrhage from Ruptured Oncotic Fusiform Aneurysms from Choriocarcinoma Metastasis Treated with Aneurysmectomy and Vessel Reconstruction

    Ito Yuichi, Araki Yoshio, Izumi Takashi, Okamoto Sho, Kimura Masaaki, Wakabayashi Toshihiko

    WORLD NEUROSURGERY   Vol. 113   page: 98 - 102   2018.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.wneu.2018.02.049

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  40. Default Mode Network Changes in Moyamoya Disease Before and After Bypass Surgery: Preliminary Report

    Sakamoto Yusuke, Okamoto Sho, Maesawa Satoshi, Bagarinao Epifanio, Araki Yoshio, Izumi Takashi, Watanabe Hirohisa, Sobue Gen, Wakabayashi Toshihiko

    WORLD NEUROSURGERY   Vol. 112   page: E652 - E661   2018.4

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    DOI: 10.1016/j.wneu.2018.01.117

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  41. Elevation of Proenkephalin 143-183 in Cerebrospinal Fluid in Moyamoya Disease. Reviewed

    Yokoyama K, Maruwaka M, Yoshikawa K, Araki Y, Okamoto S, Sumitomo M, Matsuda A, Sakamoto Y, Shimizu K, Izumi T, Wakabayashi T.

    World Neurosurg   Vol. 109   page: 446-459   2018.1

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  42. Elevation of Proenkephalin 143-183 in Cerebrospinal Fluid in Moyamoya Disease

    Yokoyama Kinya, Maruwaka Mikio, Yoshikawa Kazuhiro, Araki Yoshio, Okamoto Sho, Sumitomo Masaki, Kawamura Akino, Sakamoto Yusuke, Shimizu Kenzo, Izumi Takashi, Wakabayashi Toshihiko

    WORLD NEUROSURGERY   Vol. 109   page: E446 - E459   2018.1

  43. Rapid contralateral progression of focal cerebral arteriopathy distinguished from RNF213-related moyamoya disease and fibromuscular dysplasia. Reviewed

    Araki Y, Takagi Y, Mineharu Y, Kobayashi H, Miyamoto S, Wakabayashi T.

    Childs Nerv Syst   Vol. 33 ( 8 ) page: 1405-1409   2017.8

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  44. Rapid contralateral progression of focal cerebral arteriopathy distinguished from RNF213-related moyamoya disease and fibromuscular dysplasia

    Araki Yoshio, Takagi Yasushi, Mineharu Yohei, Kobayashi Hatasu, Miyamoto Susumu, Wakabayashi Toshihiko

    CHILDS NERVOUS SYSTEM   Vol. 33 ( 8 ) page: 1405 - 1409   2017.8

  45. Hands-on Simulation versus Traditional Video-learning in Teaching Microsurgery Technique

    Sakamoto Yusuke, Okamoto Sho, Shimizu Kenzo, Araki Yoshio, Hirakawa Akihiro, Wakabayashi Toshihiko

    NEUROLOGIA MEDICO-CHIRURGICA   Vol. 57 ( 5 ) page: 238 - 245   2017.5

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    DOI: 10.2176/nmc.oa.2016-0317

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  46. Coiling of a Ruptured Large Internal Carotid Artery Aneurysm via Extracranial-Intracranial Saphenous Vein Bypass Graft Just After Proximal Ligation of the Internal Carotid Artery

    Tajima Hayato, Araki Yoshio, Izumi Takashi, Nishihori Masahiro, Okamoto Sho, Wakabayashi Toshihiko

    WORLD NEUROSURGERY   Vol. 98   page: 879.e1-879.e4   2017.2

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    DOI: 10.1016/j.wneu.2016.11.066

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  47. 多種多様な杉田クリップの使い方について Reviewed

    岡本 奨, 清水 賢三, 村岡 真輔, 宇田 憲司, 太田 慎次, 和田 健太郎, 坂本 悠介, 荒木 芳生, 若林 俊彦

    脳卒中の外科   Vol. 45 ( 2 ) page: 83-88   2017

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  48. 小児もやもや病における周術期脳梗塞 Reviewed

    村岡真輔, 荒木芳生, 宇田憲司, 近藤五郎, 栗本路弘, 夏目敦至, 若林俊彦

    小児の脳神経     page: DOI   2017

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  49. 非もやもや病小児閉塞性脳血管障害3例の経験 Invited Reviewed

    荒木芳生, 近藤五郎, 栗本路弘, 村岡真輔, 宇田憲司, 夏目敦至, 若林俊彦.

    小児の脳神経   Vol. 41 ( 1 ) page: 135   2016.5

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KAKENHI (Grants-in-Aid for Scientific Research) 2

  1. パスウェイ異常の同定によるもやもや病発症メカニズムの解明

    Grant number:18K08967  2018.4 - 2021.3

    荒木 芳生

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    Authorship:Principal investigator 

    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

    令和1年度は成人もやもや病4症例、内頚動脈瘤4症例について、脳表血管壁および浅側頭動脈血管壁からのtotal RNA抽出、増幅complement DNAの作成、ハウスキーピング遺伝子をターゲットとしたPCRを行うことによる検体の質の評価、ラベル化cDNAの作成、Agilent Microarray へのハイブリダイゼンーション、スキャニング、統計学的解析による発現変動遺伝子の絞り込みおよびPCRによる定量を行うことができた。その結果、もやもや病の頭蓋内にて有意に高発現している1遺伝子と低発現している1遺伝子を特定した。gene set enrichment analysis では炎症にかかわる遺伝子群がもやもや病脳表血管壁では高発現であり、コラーゲン形成にかかわる遺伝子群が低発現であった。頭蓋外血管である浅側頭動脈検体における比較では明らかな発現変動遺伝子やgene set enrichment analysis において遺伝子群の変化は認めず、これらの変化はもやもや病患者の頭蓋内動脈に特異的であるものと考えている。これらの研究結果を纏めて論文化し、現在欧文誌へ投稿中である。令和2年度は小児もやもや病症例、対照として若年者のてんかん焦点切除術検体から得た脳表の血管壁についても網羅的遺伝子発現解析を行い、小児もやもや病、成人もやもや病、全年齢に特異的な発現遺伝子の検索を行う予定である。
    予定通り成人もやもや病及び非もやもや病の脳表血管壁から得た微小検体よりtotal RNAの抽出から網羅的遺伝子発現解析と、PCRによる定量により発現変動遺伝子の特定し、結果を論文にまとめ投稿するところまで進捗できている。
    令和2年度は小児もやもや病症例、対照として若年者のてんかん焦点切除術検体から得た脳表の血管壁についても網羅的遺伝子発現解析を行い、小児もやもや病、成人もやもや病、全年齢に特異的な発現遺伝子の検索を行う予定である。

  2. Epigenetic analysis of moyamoya disease

    Grant number:15K19961  2015.4 - 2018.3

    ARAKI Yoshio

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    Authorship:Principal investigator 

    Grant amount:\3900000 ( Direct Cost: \3000000 、 Indirect Cost:\900000 )

    In this study, we have tried (1) to perform comprehensive expression analysis using microarray with RNA samples extracted from intracranial and extracranial vessels of Moyamoya disease patients, (2) to verify the relationship with RNF 213 and PENK 143-183, which was identified by cerebrospinal fluid exhaustive analysis using SELDI-TOF-MS, (3) to identify specific genes involved in the development of Moyamoya disease based on transcriptional regulatory mechanism and pathway analysis by epigenetics analysis, finally (4) o create an animal model of Moyamoya disease. It was difficult to establish a methodology for RNA extraction from microscopic specimens of intracranial and extracranial blood vessels while maintaining sufficient quality. On the other hand, we performed expression analysis using microarray for RNA contained in cerebrospinal fluid of the patients with Moyamoya disease and the others, but eventually no significant results were obtained.