研究者詳細 - 永井　啓祐

論文 - 永井　啓祐

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Leaf Gas Film 1 promotes glycerol ester accumulation and formation of a tight root barrier to radial O2 loss in rice. 国際誌

Juan de la Cruz Jiménez, Siti Noorrohmah, Kiran Suresh, Viktoria V Zeisler-Diehl, Lucas León Peralta Ogorek, Max Herzog, Elisa Pellegrini, Keisuke Nagai, Motoyuki Ashikari, Hirokazu Takahashi, Ole Pedersen, Lukas Schreiber, Mikio Nakazono

Plant physiology 2024年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

Rice (Oryza sativa L.) and many other wetland plants form an apoplastic barrier in the outer parts of the roots to restrict radial O2 loss to the rhizosphere during soil flooding. This barrier facilitates longitudinal internal O2 diffusion via gas-filled tissues from shoot to root apices, enabling root growth in anoxic soils. We tested the hypothesis that Leaf Gas Film 1 (LGF1), which influences leaf hydrophobicity in rice, plays a crucial role in tight outer apoplastic barriers formation in rice roots. We examined the roots of a rice mutant (dripping wet leaf 7, drp7) lacking functional LGF1, its wild type, and an LGF1 overexpression line for their capacity to develop outer apoplastic barriers that restrict radial O2 loss. We quantified the chemical composition of the outer part of the root and measured radial O2 diffusion from intact roots. The drp7 mutant exhibited a weak barrier to radial O2 loss compared to the wild type. However, introducing functional LGF1 into the mutant fully restored tight barrier function. The formation of a tight barrier to radial O2 loss was associated with increased glycerol ester levels in exodermal cells, rather than differences in total root suberization or lignification. These results demonstrate that, in addition to its role in leaf hydrophobicity regulation, LGF1 plays an important role in controlling the function of the outer apoplastic barriers in roots. Our study suggests that increased deposition of glycerol esters in the suberized root exodermis establishes a tight barrier to radial O2 loss in rice roots.

DOI： 10.1093/plphys/kiae458

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Detection of QTLs regulating the second internode length in rice dwarf mutant d1 査読有り

Quynh T. Ha, Sandar Moe, Vincent Pamugas Reyes, Kazuyuki Doi, Kotaro Miura, Mio Mizushima, Akiteru Maeno, Katsutoshi Tsuda, Keisuke Nagai, Motoyuki Ashikari

Breeding Science 2024年

Regulator of Awn Elongation 3, an E3 ubiquitin ligase, is responsible for loss of awns during African rice domestication. 国際誌

Kanako Bessho-Uehara, Kengo Masuda, Diane R Wang, Rosalyn B Angeles-Shim, Keisuke Obara, Keisuke Nagai, Riri Murase, Shin-Ichiro Aoki, Tomoyuki Furuta, Kotaro Miura, Jianzhong Wu, Yoshiyuki Yamagata, Hideshi Yasui, Michael B Kantar, Atsushi Yoshimura, Takumi Kamura, Susan R McCouch, Motoyuki Ashikari

Proceedings of the National Academy of Sciences of the United States of America 120 巻 ( 4 ) 頁： e2207105120 2023年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Two species of rice have been independently domesticated from different ancestral wild species in Asia and Africa. Comparison of mutations that underlie phenotypic and physiological alterations associated with domestication traits in these species gives insights into the domestication history of rice in both regions. Asian cultivated rice, Oryza sativa, and African cultivated rice, Oryza glaberrima, have been modified and improved for common traits beneficial for humans, including erect plant architecture, nonshattering seeds, nonpigmented pericarp, and lack of awns. Independent mutations in orthologous genes associated with these traits have been documented in the two cultivated species. Contrary to this prevailing model, selection for awnlessness targeted different genes in O. sativa and O. glaberrima. We identify Regulator of Awn Elongation 3 (RAE3) a gene that encodes an E3 ubiquitin ligase and is responsible for the awnless phenotype only in O. glaberrima. A 48-bp deletion may disrupt the substrate recognition domain in RAE3 and diminish awn elongation. Sequencing analysis demonstrated low nucleotide diversity in a ~600-kb region around the derived rae3 allele on chromosome 6 in O. glaberrima compared with its wild progenitor. Identification of RAE3 sheds light on the molecular mechanism underlying awn development and provides an example of how selection on different genes can confer the same domestication phenotype in Asian and African rice.

DOI： 10.1073/pnas.2207105120

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Deep fluorescence observation in rice shoots via clearing technology 査読有り

184 巻 2022年6月

SNORKEL genes relating to flood tolerance were pseudogenized in normal cultivated rice 査読有り国際誌

Keisuke Nagai, Yusuke Kurokawa, Yoshinao Mori, Anzu Minami, Stefan Reuscher, Jianzhong Wu, Takashi Matsumoto, Motoyuki Ashikari

Plants 11 巻 ( 3 ) 頁： 376 - 376 2022年1月

担当区分：筆頭著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

SNORKEL1 (SK1) and SNORKEL2 (SK2) are ethylene responsive factors that regulate the internode elongation of deepwater rice in response to submergence. We previously reported that normal cultivated rice lacks SK genes because the Chromosome 12 region containing SK genes was deleted from its genome. However, no study has analyzed how the genome defect occurred in that region by comparing normal cultivated rice and deepwater rice. In this study, comparison of the sequence of the end of Chromosome 12, which contains SK genes, between normal and deepwater rice showed that complicated genome changes such as insertions, deletions, inversions, substitutions, and translocation occurred frequently in this region. In addition to SK1 and SK2 of deepwater rice, gene prediction analysis identified four genes containing AP2/ERF domains in normal cultivated rice and six in deepwater rice; we called these genes SK-LIKE (SKL) genes. SKs and SKLs were present in close proximity to each other, and the SKLs in normal cultivated rice were in tandem. These predicted genes belong to the same AP2/ERF subfamily and were separated into four types: SK1, SK2, SKL3, and SKL4. Sequence comparison indicated that normal cultivated rice possesses a gene with high homology to SK2, which we named SKL1. However, none of the predicted SKLs except for SKL3s were expressed during submergence. Although SKL3s were expressed in both normal and deepwater rice, normal rice does not undergo internode elongation, suggesting that its expression does not contribute to internode elongation. Plants overexpressing SKL1, which showed the most homology to SK2, underwent internode elongation similar to plants overexpressing SK1 and SK2 under normal growth conditions. A yeast one-hybrid assay showed that the C-end of SKL1 has transcription activity, as do the C-ends of SK1 and SK2. Our results suggested that SKLs were derived via gene duplication, but were not expressed and pseudogenized in normal cultivated rice during sequence evolution.

DOI： 10.3390/plants11030376

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Noninvasive imaging of hollow structures and gas movement revealed the gas partial-pressure-gradient-driven long-distance gas movement in the aerenchyma along the leaf blade to submerged organs in rice. 査読有り国際誌

Yong-Gen Yin, Yoshinao Mori, Nobuo Suzui, Keisuke Kurita, Mitsutaka Yamaguchi, Yuta Miyoshi, Yuto Nagao, Motoyuki Ashikari, Keisuke Nagai, Naoki Kawachi

The New phytologist 232 巻 ( 5 ) 頁： 1974 - 1984 2021年9月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Rice (Oryza sativa) plants have porous or hollow organs consisting of aerenchyma, which is presumed to function as a low-resistance diffusion pathway for air to travel from the foliage above the water to submerged organs. However, gas movement in rice plants has yet to be visualized in real time. In this study involving partially submerged rice plants, the leaves emerging from the water were fed nitrogen-13-labeled nitrogen ([13 N]N2 ) tracer gas, and the gas movement downward along the leaf blade, leaf sheath, and internode over time was monitored. The [13 N]N2 gas arrived at the bottom of the plant within 10 min, which was 20 min earlier than carbon-11 photoassimilates. The [13 N]N2 gas movement was presumably mediated by diffusion along the aerenchyma network from the leaf blade to the root via nodes functioning as junctions, which were detected by X-ray computed tomography. These findings imply the diffusion of gas along the aerenchyma, which does not consume energy, has enabled plants to adapt to aquatic environments. Additionally, there were no major differences in [13 N]N2 gas movement between paddy rice and deepwater rice plants, indicative of a common aeration mechanism in the two varieties, despite the difference in their response to flooding.

DOI： 10.1111/nph.17726

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Antagonistic regulation of the gibberellic acid response during stem growth in rice

Nagai Keisuke, Mori Yoshinao, Ishikawa Shin, Furuta Tomoyuki, Gamuyao Rico, Niimi Yoko, Hobo Tokunori, Fukuda Moyuri, Kojima Mikiko, Takebayashi Yumiko, Fukushima Atsushi, Himuro Yasuyo, Kobayashi Masatomo, Ackley Wataru, Hisano Hiroshi, Sato Kazuhiro, Yoshida Aya, Wu Jianzhong, Sakakibara Hitoshi, Sato Yutaka, Tsuji Hiroyuki, Akagi Takashi, Ashikari Motoyuki

NATURE 584 巻 ( 7819 ) 頁： 109 - + 2020年8月

Metabolite and Phytohormone Profiling Illustrates Metabolic Reprogramming as an Escape Strategy of Deepwater Rice during Partially Submerged Stress

Fukushima Atsushi, Kuroha Takeshi, Nagai Keisuke, Hattori Yoko, Kobayashi Makoto, Nishizawa Tomoko, Kojima Mikiko, Utsumi Yoshinori, Oikawa Akira, Seki Motoaki, Sakakibara Hitoshi, Saito Kazuki, Ashikari Motoyuki, Kusano Miyako

METABOLITES 10 巻 ( 2 ) 2020年2月

Evaluation of backcrossed pyramiding lines of the yield-related gene and the bacterial leaf blight resistant genes 査読有り

Shuto Yamada, Yusuke Kurokawa, Keisuke Nagai, Rosalyn B Angeles-Shim, Hideshi Yasui, Naruto Furuya, Atsushi Yoshimura, Kazuyuki Doi, Motoyuki Ashikari, Hidehiko Sunohara

18 巻頁： 18 - 28 2020年

Diel O-2 Dynamics in Partially and Completely Submerged Deepwater Rice: Leaf Gas Films Enhance Internodal O-2 Status, Influence Gene Expression and Accelerate Stem Elongation for 'Snorkelling' during Submergence 査読有り

Mori Yoshinao, Kurokawa Yusuke, Koike Masaya, Malik Al Imran, Colmer Timothy David, Ashikari Motoyuki, Pedersen Ole, Nagai Keisuke

PLANT AND CELL PHYSIOLOGY 60 巻 ( 5 ) 頁： 973-985 2019年5月

Ethylene-gibberellin signaling underlies adaptation of rice to periodic flooding

Kuroh Takeshi, Nagai Keisuke, Gamuyao Rico, Wang Diane R., Furuta Tomoyuki, Nakamori Masanari, Kitaoka Takuya, Adachi Keita, Minami Anzu, Mori Yoshinao, Mashiguchi Kiyoshi, Seto Yoshiya, Yamaguchi Shinjiro, Kojima Mikiko, Sakakibara Hitoshi, Wu Jianzhong, Ebana Kaworu, Mitsuda Nobutaka, Ohme-Takagi Masaru, Yanagisawa Shuichi, Yamasaki Masanori, Yokoyama Ryusuke, Nishitani Kazuhiko, Mochizuki Toshihiro, Tamiya Gen, McCouch Susan R., Ashikari Motoyuki

SCIENCE 361 巻 ( 6398 ) 頁： 181-185 2018年7月

Rice leaf hydrophobicity and gas films are conferred by a wax synthesis gene (LGF1) and contribute to flood tolerance

Kurokawa Yusuke, Nagai Keisuke, Phung Danh Huan, Shimazaki Kousuke, Qu Huangqi, Mori Yoshinao, Toda Yosuke, Kuroha Takeshi, Hayashi Nagao, Aiga Saori, Itoh Jun-ichi, Yoshimura Atsushi, Sasaki-Sekimoto Yuko, Ohta Hiroyuki, Shimojima Mie, Malik Al Imran, Pedersen Ole, Colmer Timothy David, Ashikari Motoyuki

NEW PHYTOLOGIST 218 巻 ( 4 ) 頁： 1558-1569 2018年6月

Time-Course Transcriptomics Analysis Reveals Key Responses of Submerged Deepwater Rice to Flooding

Minami Anzu, Yano Kenji, Gamuyao Rico, Nagai Keisuke, Kuroha Takeshi, Ayano Madoka, Nakamori Masanari, Koike Masaya, Kondo Yuma, Niimi Yoko, Kuwata Keiko, Suzuki Takamasa, Higashiyama Tetsuya, Takebayashi Yumiko, Kojima Mikiko, Sakakibara Hitoshi, Toyoda Atsushi, Fujiyama Asao, Kurata Nori, Ashikari Motoyuki, Reuscher Stefan

PLANT PHYSIOLOGY 176 巻 ( 4 ) 頁： 3081-3102 2018年4月

Breeding applications and molecular basis of semi-dwarfism in rice 査読有り

Keisuke Nagai, Ko Hirano, Rosalyn B. Angeles-Shim, Motoyuki Ashikari

Rice Genomics, Genetics and Breeding 頁： 155 - 176 2018年3月

記述言語：英語掲載種別：論文集(書籍)内論文出版者・発行元：Springer Singapore

The green revolution in rice was defined by an unprecedented increase in rice production that saved the world from an impending famine in the 1960s. Driving this revolution was the semi-dwarf 1 (sd1) gene conferring the semidwarf phenotype to the rice plant. The shorter stature conferred by sd1 gives the plants resistance to lodging even under heavy doses of nitrogen fertilizer. IR8 carrying sd1, also known as the miracle rice, was the first high-yielding rice variety that came out as a result of intensive research and breeding efforts that capitalize on the use of the semi-dwarf trait to significantly improve rice yield. Although the rice green revolution gene has been used for breeding for decades, the sd1 gene was not identified for a long time. Advancement of rice genomics facilitated the discovery that SD1 encodes the GA biosynthesis gene, GA20ox2. Genome sequencing revealed that several of the varieties used as donor lines in breeding for the semidwarf phenotype in rice possess different alleles of sd1. Apart from breeding applications, dwarf mutants have also been instrumental in uncovering the molecular mechanisms underlying gibberellin biosynthesis and signaling.

DOI： 10.1007/978-981-10-7461-5_9

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eQTLs Regulating Transcript Variations Associated with Rapid Internode Elongation in Deepwater Rice

Kuroha Takeshi, Nagai Keisuke, Kurokawa Yusuke, Nagamura Yoshiaki, Kusano Miyako, Yasui Hideshi, Ashikari Motoyuki, Fukushima Atsushi

FRONTIERS IN PLANT SCIENCE 8 巻 2017年10月

Hormone Distribution and Transcriptome Profiles in Bamboo Shoots Provide Insights on Bamboo Stem Emergence and Growth

Gamuyao Rico, Nagai Keisuke, Ayano Madoka, Mori Yoshinao, Minami Anzu, Kojima Mikiko, Suzuki Takamasa, Sakakibara Hitoshi, Higashiyama Tetsuya, Ashikari Motoyuki, Reuscher Stefan

PLANT AND CELL PHYSIOLOGY 58 巻 ( 4 ) 頁： 702-716 2017年4月

A new outlook on sporadic flowering of bamboo

Gamuyao Rico, Nagai Keisuke, Ashikari Motoyuki, Reuscher Stefan

PLANT SIGNALING & BEHAVIOR 12 巻 ( 7 ) 2017年

Construction of a versatile SNP array for pyramiding useful genes of rice 査読有り国際共著

Yusuke Kurokawa, Tomonori Noda, Yoshiyuki Yamagata, Rosalyn Angeles-Shim, Hidehiko Sunohara, Kanako Uehara, Tomoyuki Furuta, Keisuke Nagai, Kshirod Kumar Jena, Hideshi Yasui, Atsushi Yoshimura, Motoyuki Ashikari, Kazuyuki Doi

242 巻頁： 131 - 139 2016年

Loss of function at RAE2, a previously unidentified EPFL, is required for awnlessness in cultivated Asian rice 査読有り

Kanako Bessho-Uehara, Diane R. Wang, Tomoyuki Furuta, Anzu Minami, Keisuke Nagai, Rico Gamuyao, Kenji Asano, Rosalyn B. Angeles-Shim, Yoshihiro Shimizu, Madoka Ayano, Norio Komeda, Kazuyuki Doi, Kotaro Miura, Yosuke Toda, Toshinori Kinoshita, Satohiro Okuda, Tetsuya Higashiyama, Mika Nomoto, Yasuomi Tada, Hidefumi Shinohara, Yoshikatsu Matsubayashi, Anthony Greenberg, Jianzhong Wu, Hideshi Yasui, Atsushi Yoshimura, Hitoshi Mori, Susan R. McCouch, and Motoyuki Ashikari

Proc. Natl. Acad. Sci. USA 113 巻 ( 32 ) 頁： 8969 - 8974 2016年

Development of chromosome segment substitution lines harboring Oryza nivara genomic segments in Koshihikari and evaluation of yield-related traits 査読有り国際共著

66 巻 ( 5 ) 頁： 845 - 850 2016年

Convergent loss of awn in two cultivated rice species Oryza sativa and Oryza glaberrima is caused by mutations in different loci

Tomoyuki Furuta, Norio Komeda, Kenji Asano, Kanako Uehara, Rico Gamuyao, Rosalyn B. Shim-Angeles, Keisuke Nagai, Kazuyuki Doi, Diane R. Wang, Hideshi Yasui, Atsushi Yoshimura, Jianzhong Wu, Susan R. McCouch and Motoyuki Ashikari

Genes Genomes Genetics 2015年9月

Rare allele of a previously unidentified histone H4 acetyltransferase enhances grain weight, yield, acetyltransferase enhances grain weight, yield, and plant biomass in rice

Xian Jun Songa, Takehi Kuroha, Madoka Ayano, Tomoyuki Furuta, Keisuke Nagai, Norio Komeda, Shuhei Segami, Kotaro Miura, Daisuke Ogawa, Takumi Kamura, Takamasa Suzuki, Tetsuya Higashiyamad, Masanori Yamasaki, Hitoshi Mori, Yoshiaki Inukai, Jianzhong Wu, Hidemi Kitano, Hitoshi Sakakibara, Steven E. Jacobsen, and Motoyuki Ashikaria

PNAS 2015年1月

QTL analysis of internode elongation in response to gibberellin in deepwater rice.

Nagai K, Kondo Y, Kitaoka T, Noda T, Kuroha T, Angeles-Shim RB, Yasui H, Yoshimura A, Ashikari M.

AoB Plants 2014年6月

Gibberellin biosynthesis and signal transduction is essential for internode elongation in deepwater rice.

Ayano M, Kani T, Kojima M, Sakakibara H, Kitaoka T, Kuroha T, Angeles-Shim RB, Kitano H, Nagai K, Ashikari M.

Plant Cell Environment 2014年5月

Two novel QTLs regulate internode elongation in deepwater rice during the early vegetative stage.

Nagai K, Kuroha T, Ayano M, Kurokawa Y, Angeles-Shim RB, Shim JH, Yasui H, Yoshimura A, Ashikari M.

Breeding Science 2012年6月

イネの洪水における異なる2つの生存戦略

永井啓祐, 服部洋子, 芦苅基行

化学と生物 49 巻 ( 4 ) 頁： 222-224 2012年3月

Rice growth adapting to deepwater.

Hattori Y, Nagai K, Ashikari M.

Curr Opin Plant Biol. 2011年2月

Stunt or elongate? Two opposite strategies by which rice adapts to floods

Nagai K, Hattori Y, Ashikari M.

J Plant Res 2010年

The ethylene response factors SNORKEL1 and SNORKEL2 allow rice to adapt to deep water

Hattori Y, Nagai K, Furukawa S, Song XJ, Kawano R, Sakakibara H, Wu J, Matsumoto T, Yoshimura A, Kitano H, Matsuoka M, Mori H, Ashikari M.

Nature 2009年

Mapping of three QTLs that regulate internode elongation in deepwater rice

Yoko Hattori, Keisuke Nagai, Hitoshi Mori, Hidemi Kitano, Makoto Matsuoka, Motoyuki Ashikari

Breeding Science 2008年

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