Updated on 2021/08/09

写真a

 
ABE Kazuhiro
 
Organization
Cellular and Structural Physiology Institute Division Associate professor
Graduate School
Graduate School of Pharmaceutical Sciences
Title
Associate professor
Contact information
メールアドレス

Degree 1

  1. 博士(理学) ( 2004.3   北海道大学 ) 

Research Interests 1

  1. 膜タンパク質 極低温電子顕微鏡 胃 プロトンポンプ P-type ATPase

Research Areas 5

  1. Life Science / Molecular biology

  2. Life Science / Functional biochemistry

  3. Life Science / Structural biochemistry

  4. Life Science / Biophysics

  5. Life Science / Pharmaceutical chemistry and drug development sciences

Research History 6

  1. Nagoya University   Graduate School of Pharmaceutical Sciences Department of Basic Medicinal Sciences Structural Biology

    2012.4

  2. 京都大学 特定研究員

    2011.4 - 2012.3

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    Country:Japan

  3. 社団法人バイオ産業情報化コンソーシアム

    2008.4 - 2011.3

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    Country:Japan

  4. 日本学術振興会特別研究員 (PD)

    2005.4 - 2008.3

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    Country:Japan

  5. 日本学術振興会特別研究員 (PD)

    2004.4 - 2005.3

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    Country:Japan

  6. 日本学術振興会 特別研究員 (DC2)

    2003.4 - 2004.3

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    Country:Japan

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Education 3

  1. Hokkaido University   Graduate School, Division of Natural Science

    2001.4 - 2004.4

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    Country: Japan

  2. Hokkaido University   Graduate School, Division of Natural Science

    1999.4 - 2001.3

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    Country: Japan

  3. Hokkaido University   Faculty of Science

    1995.4 - 1999.3

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    Country: Japan

Professional Memberships 2

  1. 日本生化学会

  2. The Biophysical Society of Japan

Awards 5

  1. 日本生化学会奨励賞

    2013.9   日本生化学会  

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    Country:Japan

  2. 若手奨励賞

    2011.12   生体エネルギー研究会  

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    Country:Japan

  3. JBC Herbert Tabor Award

    2011.10   ASBMB  

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    Country:United States

  4. Margrethe Moller Award

    2008.8   P-ATPase conference  

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    Country:Denmark

  5. FASEB SRC Young Scientist Award

    2004.8   FASEB  

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    Country:United States

 

Papers 32

  1. Crystal structures of the gastric proton pump Reviewed International coauthorship

    Abe Kazuhiro, Irie Katsumasa, Nakanishi Hanayo, Suzuki Hiroshi, Fujiyoshi Yoshinori

    NATURE   Vol. 556 ( 7700 ) page: 214-+   2018.4

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41586-018-0003-8

    Web of Science

  2. Transport Cycle of Plasma Membrane Flippase ATP11C by Cryo-EM Reviewed

    Nakanishi Hanayo, Nishizawa Tomohiro, Segawa Katsumori, Nureki Osamu, Fujiyoshi Yoshinori, Nagata Shigekazu, Abe Kazuhiro

    CELL REPORTS   Vol. 32 ( 13 )   2020.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.celrep.2020.108208

    Web of Science

  3. Crystal structure of a human plasma membrane phospholipid flippase Reviewed

    Nakanishi Hanayo, Irie Katsumasa, Segawa Katsumori, Hasegawa Kazuya, Fujiyoshi Yoshinori, Nagata Shigekazu, Abe Kazuhiro

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 295 ( 30 ) page: 10180 - 10194   2020.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.RA120.014144

    Web of Science

  4. Isoform-selective regulation of mammalian cryptochromes Reviewed

    Miller Simon, Son You Lee, Aikawa Yoshiki, Makino Eri, Nagai Yoshiko, Srivastava Ashutosh, Oshima Tsuyoshi, Sugiyama Akiko, Hara Aya, Abe Kazuhiro, Hirata Kunio, Oishi Shinya, Hagihara Shinya, Sato Ayato, Tama Florence, Itami Kenichiro, Kay Steve A., Hatori Megumi, Hirota Tsuyoshi

    NATURE CHEMICAL BIOLOGY   Vol. 16 ( 6 ) page: 676 - +   2020.6

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41589-020-0505-1

    Web of Science

  5. Purified F-ATP synthase forms a Ca2+-dependent high-conductance channel matching the mitochondrial permeability transition pore Reviewed

    Urbani Andrea, Giorgio Valentina, Carrer Andrea, Franchin Cinzia, Arrigoni Giorgio, Jiko Chimari, Abe Kazuhiro, Maeda Shintaro, Shinzawa-Itoh Kyoko, Bogers Janna F. M., McMillan Duncan G. G., Gerle Christoph, Szabo Ildiko, Bernardi Paolo

    NATURE COMMUNICATIONS   Vol. 10   2019.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-019-12331-1

    Web of Science

  6. A single K+-binding site in the crystal structure of the gastric proton pump Reviewed

    Yamamoto Kenta, Dubey Vikas, Irie Katsumasa, Nakanishi Hanayo, Khandelia Himanshu, Fujiyoshi Yoshinori, Abe Kazuhiro

    ELIFE   Vol. 8   2019.8

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.7554/eLife.47701

    Web of Science

  7. K+ binding and proton redistribution in the E2P state of the H+, K+-ATPase Reviewed International coauthorship

    Dubey Vikas, Han Minwoo, Kopec Wojciech, Solov'yov Ilia A., Abe Kazuhiro, Khandelia Himanshu

    SCIENTIFIC REPORTS   Vol. 8   2018.8

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-018-30885-w

    Web of Science

  8. The Elusive Proton in the Gastric Proton Potassium ATPase

    Dubey Vikas, Abe Kazuhiro, Solov'yov Ilia, Khandelia Himanshu

    BIOPHYSICAL JOURNAL   Vol. 114 ( 3 ) page: 146A-146A   2018.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

  9. The cryo-EM structure of gastric H+, K+-ATPase with bound BYK99, a high-affinity member of K+-competitive, imidazo[1,2-a]pyridine inhibitors

    Abe Kazuhiro, Shimokawa Jun, Naito Mao, Munson Keith, Vagin Olga, Sachs George, Suzuki Hiroshi, Tani Kazutoshi, Fujiyoshi Yoshinori

    SCIENTIFIC REPORTS   Vol. 7   2017.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-017-06698-8

    Web of Science

  10. The cryo-EM structure of gastric H+,K+-ATPase with bound BYK99, a high-affinity member of K+-competitive, imidazo[1,2-a] pyridine inhibitors Reviewed

    Kazuhiro Abe, Jun Shimokawa, Mao Naito, Keith Munson, Olga Vagin, George Sachs, Hiroshi Suzuki, Kazutoshi Tani, Yoshinori Fujiyoshi

    Scientific Reports   Vol. 7   page: 6632   2017.6

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: DOI:10.1038/s41598-017-06698-8

  11. Cryo-electron microscopy for structure analyses of membrane proteins in the lipid bilayer Invited Reviewed

    Abe K. & Fujiyoshi Y

    Current Opinion in Structural Biology   Vol. 39   page: 71-78   2016.8

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.sbi.2016.06.001

  12. Systematic comparison of molecular conformations of H+,K+-ATPase reveals an important contribution of the A-M2 linker for the luminal gating. Reviewed

    Abe, K., Tani, K., Fujiyoshi, Y.

    J. Biol. Chem.   Vol. 289 ( 44 ) page: 30590-30601   2014.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  13. Biochemical and electron crystallographic studies of gastric proton pump Invited

    Abe, K.

      Vol. 86 ( 4 ) page: 429-440   2014.8

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    Authorship:Lead author   Language:Japanese  

  14. 1. The four-transmembrane protein IP39 of Euglena forms strands by a trimeric unit repeat Reviewed

    Suzuki, H., Ito, Y., Yamazaki, Y., Mineta, K., Uji, M., Abe, K., Tani, K., Fujiyoshi, Y. & Tsukita, S.

    Nature Communications   Vol. 4   page: 1766   2013.4

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    Language:English   Publishing type:Research paper (scientific journal)  

  15. Carbon sandwich preparation preserves quality of two-dimensional crystals for cryo-electron microscopy Reviewed

    Yang F., Abe, K., Tani K., Fujiyoshi Y.

    Microscopy   Vol. 62   page: 597-606   2013

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  16. Cryo-EM structure of gastric H+,K+-ATPase with a single occupied cation-binding site Reviewed

    Abe, K., Tani, K., Friedrich, T.& Fujiyoshi, Y.

    Proceedings of the National Academy of Sciences USA   Vol. 109 ( 45 ) page: 18401-18406   2012.11

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  17. Unique properties of gastric H+,K+-ATPase and conserved conformational changes among P-type ATPases

    Abe, K.

      Vol. 84   page: 115-119   2012.2

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    Language:Japanese  

  18. Conformational rearrangement of gastric H+,K+-ATPase with an acid suppressant.

    Abe, K., Tani, K. & Fujiyoshi, Y.

    Nature Communications   Vol. 2 ( 155 ) page: 1-9   2011.1

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    Language:English   Publishing type:Research paper (scientific journal)  

  19. A novel ratchet mechanism of gastric H+,K+-ATPase revealed by electron crystallography of two-dimensional crystals.

    Abe, K., Tani, K., Nishizawa, T. & Fujiyoshi, Y.

      Vol. 130   page: 205-210   2010

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    Language:Japanese  

  20. Structural analysis of gastric proton pump by cryo-electron microscopy.

    Abe, K.

      Vol. 45   page: 264-267   2010

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    Language:Japanese  

  21. Structural and functional characterization of H+,K+-ATPase with bound fluorinated phosphate analogs.

    Abe, K., Tani, K. & Fujiyoshi, Y.

    Journal of Structural Biology   Vol. 170   page: 60-68   2010

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  22. Inter-subunit interaction of gastric H+,K+-ATPase prevents reverse reaction of the transport cycle.

    Abe, K., Tani, K., Nishizawa, T. & Fujiyoshi, Y.

    EMBO Journal   Vol. 28   page: 1637-1643   2009

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  23. E2P state stabilization by the N-terminal tail of the H,K-ATPase beta-subunit is critical for efficient proton pumping under in vivo conditions.

    Dürr, K., Abe, K., Tavraz, N.N. & Friedrich, T.

    Journal of Biological Chemistry   Vol. 284   page: 20147-20154   2009

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    Language:English   Publishing type:Research paper (scientific journal)  

  24. Epigallocatechin-3-gallate is an inhibitor of Na+,K+-ATPase by favoring the E1 conformation.

    Ochiai, H., Takeda, K., Soeda, S., Tahara, Y., Takenaka, H., Abe, K., Hayashi, Y., Noguchi, S., Inoue, M., Schwarz, S., Schwarz, W. & Kawamura, M.

    Biochemical Phamacology   Vol. 78   page: 1069-1074   2009

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    Language:English   Publishing type:Research paper (scientific journal)  

  25. Structural analysis of 2D crystals of gastric H+,K+-ATPase in different states of the transport cycle.

    Nishizawa, T., Abe, K., Tani, K. & Fujiyoshi, Y.

    Journal of Structural Biology   Vol. 162   page: 219-228   2008

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    Language:English   Publishing type:Research paper (scientific journal)  

  26. Relationship between activity and tetraprotomeric structure of ion-transporting ATPases.

    Abe, K., & Kaya, S.

      Vol. 79   page: 527-534   2007

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    Language:Japanese  

  27. New evidence for ATP binding induced catalytic subunit interactions in pig kidney Na/K-ATPase.

    Tanoue, K., Kaya, S., Hayashi, Y., Abe, K., Imagawa, T., Taniguchi, K. & Sakaguchi, K.

    Journal of Biochemistry   Vol. 140   page: 599-607   2006

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    Language:English   Publishing type:Research paper (scientific journal)  

  28. Evidence for a relationship between activity and the tetraprotomeric assembly of solubilized pig gastric H/K-ATPase.

    Abe, K., Kaya, S., Taniguchi, K., Hayashi, Y., Imagawa, T., Kikumoto, M., Oiwa, K. & Sakaguchi, K.

    Journal of Biochemistry   Vol. 138   page: 293-301   2005

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    Language:English   Publishing type:Research paper (scientific journal)  

  29. Correlation between the activities and the oligomeric forms of pig gastric H/K-ATPase.

    Abe, K., Kaya, S., Hayashi, Y., Imagawa, T., Kikumoto, M., Oiwa, K., Katoh, T., Yazawa, M. & Taniguchi, K.

    Biochemistry   Vol. 42   page: 15132-15138   2003

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    Language:English   Publishing type:Research paper (scientific journal)  

  30. Gastric H/K-ATPase liberates two moles of Pi from one mole of phosphoenzyme formed from a high-affinity ATP binding site and one mole of enzyme-bound ATP at the low-affinity site during cross-talk between catalytic subunits.

    Abe, K., Kaya, S., Imagawa, T. & Taniguchi, K.

    Biochemistry   Vol. 41   page: 2438-2445   2002

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  31. The oligomeric nature of Na/K-transport ATPase

    Taniguchi, K, Kaya, S., Abe, K. & Mårdh, S.

    Journal of Biochemistry   Vol. 129   page: 335-342   2001

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  32. Acid-labile ATP and/or ADP/Pi binding to the tetraprotoemeric form of Na/K-ATPase accompanying catalytic phosphorylation-dephosphorylation cycle.

    Yokoyama, T., Kaya, S., Abe, K., Taniguchi, K., Katoh, T., Yazawa, M., Hayashi, Y. & Mårdh, S.

    Journal of Biological Chemistry   Vol. 274   page: 31792-31796   1999

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Books 2

  1. Methods in Molecular Biology, P-type ATPases, 4 Isoration of H+,K+-ATPase-enriched membrane fraction from pig stomach

    ( Role: Joint author)

    Springer  2016.1  ( ISBN:978-1-4939-3178-1

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    Language:English

  2. Methods in Molecular Biology, P-type ATPases, 39 Two-dimensional crystallization of gastric H+,K+-ATPase for structural analysis by electron crystallography

    Abe K( Role: Sole author)

    Springer  2016.1  ( ISBN:978-1-4939-3178-1

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    Language:English

Presentations 62

  1. 『細胞死を司るポンプ』脂質フリッパーゼATP11Cの結晶構造とCryo-EM構造

    阿部一啓

    日本生体エネルギー研究会第46回討論会 

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    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  2. 細胞膜フリッパーゼATP11Cの構造機能解析

    阿部一啓

    第93回日生化学会 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜(Zoom)   Country:Japan  

  3. Crystal structures of the gastric proton pump - molecular basis for the binding of acid suppressants, H+-extrusion and K+-counter-transport

    Kazuhiro Abe

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    Event date: 2020.3

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  4. Structural basis of the proton extrusion mechanism of the gastric proton pump Invited

    Kazuhiro Abe

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    Event date: 2020.3

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  5. Molecular mechanisms for the drug binding, H+-extrusion and the K+ counter-transport Invited International conference

    Kazuhiro Abe

    Academia Sinica IBC seminar 

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    Event date: 2019.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Academia Sinica, Taipei   Country:Taiwan, Province of China  

  6. Crystal structure of the gastric proton pump Invited International conference

    Kazuhiro Abe

    World Laureates Forum 2nd conference 

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    Event date: 2019.10

    Language:English   Presentation type:Poster presentation  

    Venue:Shanghai, China   Country:China  

  7. X線結晶学とクライオ電顕で得られた構造情報の上手な活用法 ~胃プロトンポンプの構造解析を例として~ Invited International conference

    阿部一啓

    2019年度生理研研究会 

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    Event date: 2019.9 - 2019.10

    Language:Japanese   Presentation type:Oral presentation (keynote)  

    Venue:大阪   Country:Japan  

  8. 胃プロトンポンプの輸送機構に対する構造基盤 Invited International conference

    阿部一啓

    生物物理第57回年会 台湾‐日本 二国間シンポジウム:X線結晶構造解析とクライオ電顕2019 

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    Event date: 2019.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:宮崎   Country:Japan  

  9. Structural basis for the H+-extrusion and the K+-occlusion of the gastric proton pump Invited International conference

    Abe K

    Society of General Physiology /SOBLA 2019 

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    Event date: 2019.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Valparaiso, Chile   Country:Chile  

  10. Crystal structure of the gastric proton pump International conference

    Kazuhiro Abe, Kenta Yamamoto, Katsumasa Irie, Hanayo Nakanishi, Yoshinori Fujiyoshi

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    Event date: 2019.7

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Colby Sawyer, NH, USA   Country:United States  

  11. 胃の強酸性化の分子メカニズム ‐胃プロトンポンプの結晶構造解析 Invited

    阿部一啓

    日本生体エネルギー研究会 第44回討論会 

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    Event date: 2018.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:千葉大学   Country:Japan  

  12. Crystal structures of the gastric proton pump Invited International conference

    Kazuhiro Abe

    AsCA2018 

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    Event date: 2018.12

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Auckland, NZ   Country:Japan  

  13. 4. 胃プロトンポンプの構造生理学~胃酸に対してプロトンを押し出すメカニズム~ Invited

    阿部一啓

    大阪大学蛋白質研究所セミナー 構造情報に基づいた膜イオン輸送タ ンパク質の生理機能の解明に向けて 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  14. 1. 胃プロトンポンプの結晶構造によって明らかになったH+排出機構 Invited

    阿部一啓

    大阪大学蛋白質研究所セミナー 構造生物学と計算科学の融合による動的構造生物学の新しい展開  

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  15. 胃プロトンポンプの結晶構造 International conference

    阿部一啓

    第91回日本生化学会大会 シンポジウム 生化学から広がる膜輸送体研究の深化, 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  16. 3. 胃の強酸性化の秘密 Invited

    阿部一啓

    創薬等ライフサイエンス研究支援基盤事業 平成30年度BINDS公開 シンポジウム「知って、使って、進む あなたの研究」 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  17. Structural basis for the H+ extrusion and the transport stoichiometry of the gastric proton pump Invited

    Kazuhiro Abe

    Frontiers in P-type ATPase Research 2018, Institute for Quantitative Bioscience 

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    Event date: 2018.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:The University of Tokyo   Country:Japan  

  18. Crystal structure of the gastric proton pump at 2.8A resolution

    Kazuhiro Abe

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    Event date: 2017.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  19. Structural and functional analysis of gastric proton pump and acid suppressants

    Kazuhiro Abe

    ConBio2017 

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    Event date: 2017.12

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  20. Structural and functional analysis of gastric H+,K+-ATPase Invited International conference

    The 15th international conference on Na,K-ATPase and related transport ATPases 

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    Event date: 2017.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Otsu, Japan   Country:Japan  

  21. 胃プロトンポンプの構造生理学 Invited

    阿部一啓

    日本物理学会 第72回年次大会 

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:大阪大学   Country:Japan  

  22. Binding model of the acid suppressant to the gastric proton pump Invited

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    Event date: 2016.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:筑波   Country:Japan  

  23. 日本生化学会 Invited

    阿部一啓

    第89回日本生化学会大会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:東北大学   Country:Japan  

  24. 電子線結晶学と機能解析に基づいた胃プロトンポンプの構造生理学 Invited

    阿部一啓

    大阪大学蛋白質研究所セミナー 『膜タンパク質の構造ダイナミクス』 Structural dynamics of membrane proteins 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:大阪大学   Country:Japan  

  25. 胃プロトンポンプの構造生理学 Invited

    阿部一啓

    千里ライフサイエンスセミナーK2 トランスポーターと創薬~構造と病態からのアプローチ~ 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:千里ライフサイエンスセンタービル 5階 山村雄一記念ライフホール   Country:Japan  

  26. Electron crystallographic analysis of gastric proton pump

    Kazuhiro Abe

    IPR Seminar "Introduction and overview of cryo-electron microscopy" 

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    Event date: 2016.2

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  27. 胃H+,K+-ATPaseの 胃酸抑制剤結合状態についての考察

    阿部一啓

    日本生体エネルギー研究会 第41回 討論会 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  28. Mutational analysis of acid surppressant binding site of gastric proton pump based on its structural model

    Kazuhiro ABE, Kazutoshi Tani, Mao Naito, Jun Shimokawa, Yoshinori Fujiyoshi

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    Event date: 2015.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  29. Antagonist-bound structure of gastric proton pump

    Abe K

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    Event date: 2015.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  30. Structural physiology of gastric proton pump International conference

    Denmark-Japan Joint Workshop on Ion Transport Proteins 

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    Event date: 2015.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  31. Electron crystallographic and mutational analysis of acid suppressant-bound gastric proton pump

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    Event date: 2015.5 - 5015.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  32. Systematic comparison of the molecular conformation of gastric H+,K+-ATPase in E2P state analogs

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    Event date: 2014.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  33. 胃プロトンポンプH+,K+-ATPaseの構造生理学

    阿部一啓

    第3回 九州地区 生理薬理系研究会 

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    Event date: 2014.12

    Language:Japanese   Presentation type:Oral presentation (keynote)  

    Country:Japan  

  34. Electron crystallographic analysis of gastric proton pump

    Abe, K.

    JEM Next-Generation Microscopic Science 

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    Event date: 2014.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  35. Systematic comparison of the molecular conformation of gastric H+,K+-ATPase in E2P state analogs

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    Event date: 2014.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  36. Structural and biochemical analysis of gastric H+,K+-ATPase in E2P-analogue conformations International conference

    Abe, K.

    ASBMB Special symposia, Na,K-ATPase and related transport ATPases: Structure, Mechanism, Cell Biology, Health and Disease 

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    Event date: 2014.8 - 2014.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Netherlands  

  37. 胃プロトンポンプの構造生理学 International conference

    阿部一啓

    第9回トランスポーター研究会 

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    Event date: 2014.6

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  38. 胃プロトンポンプの阻害剤結合構造から見えてきたE2P遷移状態における構造変化

    阿部一啓

    日本生体エネルギー研究会第39回討論会 

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    Event date: 2013.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:静岡   Country:Japan  

  39. 胃プロトンポンプの極低温電子顕微鏡による構造解析

    阿部一啓

    第4回神経科学と構造生物学の融合研究会 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:岡崎コンファレンスセンター   Country:Japan  

  40. 電子顕微鏡のイメージから膜タンパク質の立体構造を再構成する~胃プロトンポンプの例を中心として~

    阿部一啓

    分析化学会中部支部愛知地区講演会 

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    Event date: 2013.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋大学   Country:Japan  

  41. Biochemical and electron crystallographic studies of gastric proton pump

    Kazuhiro Abe

    The 86th Annual Meeting of the Japanese Biochemical Society 

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    Event date: 2013.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  42. 胃プロトンポンプの生化学と電子線結晶構造解析

    阿部一啓

    第86回日本生化学会 奨励賞受賞講演 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:横浜   Country:Japan  

  43. Cryo-EM structures of gastric proton pump International conference

    Abe K

    FAOBMB mini-symposium 

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    Event date: 2013.4

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  44. Unique properties and conserved conformational changes found in gastric H+,K+-ATPase International conference

    Abe K, Tani K and Fujiyoshi Y

    Nagoya Symposium 

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    Event date: 2013.1

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  45. 電子線結晶学による胃プロトンポンプの立体構造解析 International conference

    阿部一啓

    生体エネルギー研究会第38回討論会 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:岡山大学   Country:Japan  

  46. 胃H+,K+-ATPaseの逆反応防止機構とその解除-- (K+)E2~P構造を中心として

    阿部一啓

    生体エネルギー研究会 第37回討論会 

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    Event date: 2011.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都産業大学 京都  

  47. Unique structures and conserved conformational changes found in gastric H+,K+-ATPase International conference

    Kazuhiro Abe

    2011 ASBMB sepcial symposia, Na/K-ATPase and related P-ATPases 

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    Event date: 2011.9 - 2011.10

    Language:English   Presentation type:Oral presentation (invited, special)  

  48. 電子線結晶学によって明らかになった胃H+,K+-ATPaseのユニークな構造とP型ATPaseファミリー間で保存された構造変化

    阿部一啓

    第84回日本生化学会大会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都国際会議場 京都  

  49. Conformational rearrangement of gastric H+,K+-ATPase induced by an acid suppressant International conference

    Kazuhiro Abe

    Direct imaging in Bio/Medical science 

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    Event date: 2011.1

    Language:English   Presentation type:Poster presentation  

  50. Three-dimensional structure of gastric H+,K+-ATPase with unti-ulcer drug International conference

    Kazuhiro Abe

    FASEB SRC, transport ATPase 

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    Event date: 2010.6

    Language:English   Presentation type:Oral presentation (general)  

  51. 電子線結晶学により明らかになった胃プロトンポンプの逆反応防止機構

    阿部一啓

    生体エネルギー研究会 第35回討論会 

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    Event date: 2009.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:旭川医科大学  

  52. 胃プロトンポンプのサブユニット間相互作用による逆反応防止機構

    阿部一啓

    第82回日本生化学会大会 

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    Event date: 2009.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:神戸国際会議場  

  53. A Novel Ratchet Mechanism of Gastric H+,K+-ATPase Revealed by Cryo-Electron Microscopy

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    Event date: 2009.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  54. A Novel Ratchet Mechanism of Gastric H+,K+-ATPase Revealed by Electron Crystallography of Two-Dimensional Crystals

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    Event date: 2009.1

    Language:Japanese   Presentation type:Oral presentation (general)  

  55. Three-dimensional structure of gastric H/K-ATPase at 6.5 Å resolution determined by electron crystallography of two-dimensional crystals International conference

    Kazuhiro Abe, Kazutoshi Tani, Tomohiro Nishizawa, Yoshinori Fujiyoshi

    17th Meeting of Methods in Protein Structure Analysis (MPSA2008)  

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    Event date: 2008.8

    Language:English   Presentation type:Oral presentation (invited, special)  

  56. Structure of gastric H+,K+-ATPase at 8-Å resolution International conference

    Kazuhiro Abe

    12th International PATPase conference, Na,K-ATPase and Related Transport ATPases 

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    Event date: 2008.8

    Language:English   Presentation type:Oral presentation (invited, special)  

  57. Structural Analysis of 2D crystals of Gastric H+, K+-ATPase in different states of the transport cycle

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    Event date: 2007.12

    Language:Japanese   Presentation type:Oral presentation (general)  

  58. Two-dimensional crystallization of pig gastric H/K-ATPase International conference

    Kazuhiro Abe, Tomohiro Nishizawa, Kazutoshi Tani, Yoshinori Fujiyoshi

    The 16th International Microscopy Congress 

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    Event date: 2006.9

    Language:English   Presentation type:Poster presentation  

  59. Two-dimensional Crystallografic Study of Pig Gastric H/K-ATPase International conference

    Kazuhiro Abe, Tomohiro Nishizawa, Kazutoshi Tani, Yoshinori Fujiyoshi

    IUBMB Cation pump meeting 

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    Event date: 2006.7

    Language:English   Presentation type:Poster presentation  

  60. K+-induced change in oligomeric assembly of C12E8-solubilized pig gastric H/K-ATPase observed by single-molecule detection technique. International conference

    Kazuhiro Abe, Shunji Kaya, Kazuyashu Sakaguchi, Toshiaki Imagawa, Yutaro Hayashi, Kazuya Taniguchi

    Na,K-ATPase & Related Cation Pumps 

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    Event date: 2005.9

    Language:English   Presentation type:Poster presentation  

  61. Investigation of tetraprotomeric structure of pig gastric H/K-ATPase based on single-molecule detection techniques International conference

    Abe K, Kaya S, Kikumoto M, Oiwa K, Katoh T, Yazawa M and Taniguchi K

    FASEB Summer Research Conference, Transport ATPases: Genomics to Mechanism and Releavnce to Diseases 

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    Event date: 2003.7

    Language:English   Presentation type:Oral presentation (general)  

  62. K+ induced simultaneous liberation of two moles of Pi one from mole of EP and the other from EATP of oligomeric H/K-ATPase from pig stomach International conference

    Kazuhiro Abe, Shunji Kaya, Toshiaki Imagawa, Kazuya Taniguchi

    10th International Conference on Na/K-ATPase and Related Cation Pumps 

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    Event date: 2002.8

    Language:English   Presentation type:Oral presentation (invited, special)  

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Research Project for Joint Research, Competitive Funding, etc. 8

  1. 胃酸分泌の分子メカニズム解明と新規薬剤開発の為の構造基盤

    2018.11 - 2021.3

    武田科学振興財団   生命科学研究助成 

    阿部一啓

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\10000000

  2. 細胞膜におけるリン脂質の非対称分布とその崩壊

    2014.10 - 2020.3

    CREST 

    阿部一啓

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\51000000

  3. 『細胞死を司るポンプ』脂質フリッパーゼのリン脂質認識と輸送機構

    2021.3 - 2022.3

    公益財団法人 小野医学研究財団  2020年度 小野医学研究助成 

    阿部一啓

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\2000000

  4. 細胞膜を隔てた物質非対称分布を作り出す能動輸送体の作動機構の解明

    2020.12 - 2022.3

    大幸財団 自然科学系学術研究助成 

    阿部一啓

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4000000

  5. 胃プロトンポンプを初めとしたヒト能動輸送体の構造機能解析と創薬への展開

    2019.12 - 2021.9

    内藤記念科学奨励金・研究助成 

    阿部一啓

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    Grant type:Competitive

  6. 物質不均衡を司る能動輸送体の構造解析と創薬

    2019.12 - 2021.4

    上原記念生命科学財団 研究助成金 

    阿部一啓

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    Grant type:Competitive

  7. クライオ電子顕微鏡のフィードバックに基づく膜タンパク質複合体の生産と技術支援

    2017.4 - 2022.3

    創薬等ライフサイエンス研究支援基盤事業 

    阿部一啓

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\275000000

  8. 多様な顕微鏡技術による膜タンパク質複合体の多階層での機能構造研究

    2012.10 - 2017.3

    創薬等支援技術基盤プラットフォーム補助金 

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    Grant type:Competitive

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KAKENHI (Grants-in-Aid for Scientific Research) 8

  1. 物質不均衡分布を司る能動輸送体P型ATPaseの構造機能生理学

    Grant number:21H02426  2021.4 - 2024.3

    阿部 一啓

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    Authorship:Principal investigator 

    Grant amount:\17550000 ( Direct Cost: \13500000 、 Indirect Cost:\4050000 )

  2. 胃プロトンポンプの構造生理学研究

    2017.4 - 2021.3

    科学研究費補助金  基盤研究(B)

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    Authorship:Principal investigator 

  3. 胃プロトンポンプの構造生理学研究

    Grant number:17H03653  2017.4 - 2021.3

    阿部 一啓

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    Authorship:Principal investigator 

    Grant amount:\17030000 ( Direct Cost: \13100000 、 Indirect Cost:\3930000 )

    当該年度は、HKATPaseの輸送化学量論を明らかにするために、輸送イオンが閉塞さらた状態における構造解析を行った。複数の条件で得られた結晶構造から、HKATPaseは対向輸送するK+を1つだけ結合することを明らかにした。輸送に起電性が無いことから、同様に1つだけH+を輸送するとこが考えられる。この化学量論は、限られたATPの加水分解から得られる自由エネルギーを用いて、胃の内部にpH 1 = 100万倍のH+濃度勾配を形成することと矛盾しない。この分子の発見以来40年以上も決着がついていなかった輸送化学量論に決着をつることができた。
    上記の結果は、機能解析や計算機シミュレーションの結果と併せて論文として発表した。
    研究目的に掲げる、HKATPaseの複数の構造解析から、その生理機能に重要な情報を得るという方向性においては、当初の想定以上の成果を挙げている。
    未発表ながらCryo-EMによる構造解析も進めており、こちらも想定以上の成果を上げつつある。
    HK alpha2の構造解析に関しては、三次元結晶は得られているものの、分解能が制限されており、コンストラクトや発現系の改良が急務である。
    世界市場規模が2兆円とも言われる胃酸抑制剤は、多くの方々のQOLの維持に重要である。しかしんがら、胃癌の原因となるピロリ菌の除菌に際しても使用されることや、胃酸抑制剤が膵臓がん由来細胞に対して有効であるという報告もあり、更なる改良や新規薬剤開発に資する構造情報は有用であると考えられる。複数の胃酸抑制剤の結合構造の解析と、そこから得られる薬剤結合に必要な構造ファクターを抽出することで、より良い薬剤開発へと繋がる研究を展開していく。
    CryoEMによる構造解析を推進し、これまで得られていない中間体の構造を得ることで、HKATPaseや他の能動輸送体の作動機構の理解を進める。

  4. Studies in structural physiology of channels

    Grant number:15H05775  2015.5 - 2020.3

    FUJIYOSHI Yoshinori

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    Authorship:Coinvestigator(s) 

    Structural information of membrane proteins including channels is crucial toward gaining a better understanding of biologic functions. The information that can be gained from a protein structure strongly depends on its resolution - the higher the resolution, the more insight gained into the structure-function relationship of biologic macromolecules. We analyzed structures of proton pump, water channel AQP4, whose inhibitor could be a drug for brain edema, Na+ channel, gap junction channels and tight junction channels at high resolutions. While we could not get significant results of AChR, we analyzed structures of endothelin receptor and its complexes with the agonist and antagonists. In this project, we could get important knowledge in the research field named as structural physiology and partly elucidate how the channels work and regulate functions of the human body. We also contributed advancement of cryo-electron microscopy by developing cryo-electron microscopes.

  5. 最大級のイオン濃度勾配を形成する胃プロトンポンプ作動機構の解明

    2014.4 - 2018.3

    科学研究費補助金  若手研究(A)

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    Authorship:Principal investigator 

  6. 電子線結晶学と酵素化学的研究に基づいた胃プロトンポンプのユニークな作動機構の解明

    2012.4 - 2014.3

    科学研究費補助金 

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    Authorship:Principal investigator 

  7. 4量体H/K-ATPaseの構造機能連関~4量体構造の生理的意義の解明~ (1701307)

    2006.4 - 2009.3

    科学研究費補助金 

    阿部 一啓

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    Authorship:Principal investigator 

  8. P型‐ATPaseの多量体構造と機能の相関 (1509844)

    2004.4 - 2006.3

    科学研究費補助金 

    阿部 一啓

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    Authorship:Principal investigator 

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Teaching Experience (On-campus) 37

  1. Introduction to Biochemistry III

    2020

  2. Structural Biology Research Course

    2020

  3. Molecular & Cellular Search and Production of Bioactive Factors

    2020

  4. Advanced Structural Biophysics

    2020

  5. Basic Training for Crossover Research

    2020

  6. 先端構造科学

    2018

  7. 多分野融合実践演習

    2018

  8. 多分野融合実践実習

    2018

  9. 創薬分子構造学実験

    2018

  10. 創薬分子構造学セミナー1B

    2018

  11. 創薬探索科学

    2018

  12. 創薬分子構造学セミナー1A

    2018

  13. 創薬分子構造学セミナー1A

    2017

  14. 多分野融合実践演習

    2017

  15. 多分野融合実践実習

    2017

  16. 創薬分子構造学実験

    2017

  17. 創薬分子構造学セミナー1B

    2017

  18. 多分野融合実践実習

    2016

  19. 創薬分子構造学実験

    2016

  20. 創薬分子構造学セミナー1B

    2016

  21. 創薬分子構造学セミナー1A

    2016

  22. 多分野融合実践演習

    2016

  23. 多分野融合実践演習

    2015

  24. 多分野融合実践実習

    2015

  25. 創薬分子構造学実験

    2015

  26. 創薬分子構造学セミナー1B

    2015

  27. 創薬分子構造学セミナー1A

    2015

  28. 創薬分子構造学セミナー1A

    2014

  29. 創薬分子構造学セミナー1B

    2014

  30. 創薬分子構造学実験

    2014

  31. 多分野癒合実践実習

    2014

  32. 多分野融合実践演習

    2014

  33. 多分野融合実践演習

    2013

  34. 創薬分子構造学実験

    2013

  35. 創薬分子構造学セミナー1B

    2013

  36. 創薬分子構造学セミナー1A

    2013

  37. 多分野癒合実践実習

    2013

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Teaching Experience (Off-campus) 2

  1. 分子細胞生物学

    2021.1 Ehime University)

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    Level:Undergraduate (specialized) 

  2. 基盤物理化学特論

    2020.6 Kyoto University)

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    Level:Postgraduate