Faculty Profiles - TATSUKAWA Hideki

Papers - TATSUKAWA Hideki

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Transglutaminase orthologues in medaka fish - biochemical characterization and establishment of gene-deficient mutants. Reviewed International journal

Qi Meng, Yuko Watanabe, Risa Suzuki, Rina Oguri, Hideki Tatsukawa, Kiyotaka Hitomi

Analytical biochemistry Vol. 604 page： 113610 - 113610 2020.9

Language：Japanese Publishing type：Research paper (scientific journal)

By genome analysis, seven homologous genes (orthologues) of human transglutaminases (TGases) have been identified in medaka fish (Oryzias latipes), some of which clearly corresponded to the Factor XIII, TG1, and TG2. The enzymatically active-recombinant proteins for these medaka TGases has been successfully produced in bacteria or baculovirus-infected insect cell systems. Specific antibodies have been prepared and used in immunohistochemical analyses to reveal tissue distribution. Furthermore, gene-deficient medaka mutants for the genes encoding Factor XIII and TG1 have been established with analysis of their phenotypes. Retarded cross-linking of fibrin and higher sensitivity to osmolality are observed in which each gene had been knock-out. In this review, we summarize these biochemical features and the phenotypes of these gene-deficient fish.

DOI： 10.1016/j.ab.2020.113610

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PubMed

Gene disruption of medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2) causes movement retardation. Reviewed International journal

Yuko Watanabe, Kazuho Okuya, Yuki Takada, Masato Kinoshita, Saori Yokoi, Shinichi Chisada, Yasuhiro Kamei, Hideki Tatsukawa, Naoyuki Yamamoto, Hideki Abe, Hisashi Hashimoto, Kiyotaka Hitomi

Journal of biochemistry Vol. 168 ( 3 ) page： 213 - 222 2020.9

Language：Japanese Publishing type：Research paper (scientific journal)

Transglutaminases (TGases) are an enzyme family that catalyzes protein cross-linking essential for several biological functions. In the previous studies, we characterized the orthologues of the mammalian TGase family in medaka (Oryzias latipes), an established fish model. Among the human isozymes, tissue-type transglutaminase (TG2) has multiple functions that are involved in several biological phenomena. In this study, we established medaka mutants deficient for the orthologue of human TG2 (OlTGT) using the CRISPR/Cas9 and TALEN systems. Although apparent morphological changes in the phenotype were not observed, movement retardation was found in the mutant fish when evaluated by a tank diving test. Furthermore, comparative immunohistochemistry analysis using in this fish model revealed that OlTGT was expressed at the periventricular layer of the optic tectum. Our findings provide novel insight for the relationship between tissue-type TGase and the nervous system and the associated behavior.

DOI： 10.1093/jb/mvaa038

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PubMed

Analysis of the expression of transglutaminases in the reconstructed human epidermis using a three-dimensional cell culture. Reviewed International journal

Hirofumi Teshima, Manami Kato, Hideki Tatsukawa, Kiyotaka Hitomi

Analytical biochemistry Vol. 603 page： 113606 - 113606 2020.8

Language：Japanese Publishing type：Research paper (scientific journal)

The skin epidermis functions as a barrier to various external stresses. In the outermost layer, the terminally differentiated keratinocytes result in cornification with tough structure by formation of cornified envelope beneath the plasma membrane. To complete the formation of the cornified envelope, several structural proteins are cross-linked via the catalytic action of transglutaminases (TG1, TG3, TG5, and TG6). The expression and activation of these enzymes are regulated in a tightly coordinated manner during keratinocyte differentiation. We here show the system detecting the activity of the TGases using specific glutamine-donor substrate peptides in a three-dimensional culture system of keratinocytes. In this review, we summarize the roles of the epidermal enzymes and introduce a detection method that will provide a system for evaluating the skin barrier function.

DOI： 10.1016/j.ab.2020.113606

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PubMed

抗体型糸球体腎炎におけるタンパク質架橋化酵素トランスグルタミナーゼの機能

伊藤辰将, 辰川英樹, 横江優貴, 高橋和男, 丸山彰一, 湯澤由紀夫, 人見清隆, 坪井直毅

日本腎臓学会誌 Vol. 62 ( 4 ) page： 270 - 270 2020.7

タンパク質架橋酵素を介した上皮細胞の間葉転換機構の解析 Invited Reviewed

辰川英樹, 篠田祥希, 竹内大修, 人見清隆

日本応用酵素協会誌 Vol. 55 page： 1 - 9 2020

タンパク質の架橋による翻訳後修飾反応を介した組織線維化の分子メカニズムの解析

辰川英樹, 竹内大修, 伊藤辰将, 人見清隆

日本生化学会大会プログラム・講演要旨集 Vol. 92回 page： [3T09m - 04] 2019.9

タンパク質の架橋による翻訳後修飾反応を介した組織線維化の分子メカニズムの解析

辰川英樹, 竹内大修, 伊藤辰将, 人見清隆

日本生化学会大会プログラム・講演要旨集 Vol. 92回 page： [3T09m - 04] 2019.9

Studies on differentiation-dependent expression and activity of distinct transglutaminases by specific substrate peptides using three-dimensional reconstituted epidermis. Reviewed International journal

Yuki Tanabe, Miki Yamane, Manami Kato, Hirofumi Teshima, Miki Kuribayashi, Hideki Tatsukawa, Hiroyuki Takama, Masashi Akiyama, Kiyotaka Hitomi

The FEBS journal Vol. 286 ( 13 ) page： 2536 - 2548 2019.7

Language：Japanese Publishing type：Research paper (scientific journal)

During skin formation, particularly during differentiation of keratinocytes, unique post-translational modifications play a role in forming a proteinaceous supermolecule called the cornified envelope (CE), which is necessary for barrier function. Transglutaminases (TGs) are essential enzymes involved in the cross-linking of various keratinocyte structural proteins to complete CE formation. The TG family consists of eight isozymes, with two members, TG1 and TG3, located mainly in the epidermis. In an in vitro three-dimensional (3D) culture system, reconstruction of the epidermis allows cornification of the terminally differentiated keratinocytes. In this study, using isozyme-specific substrate peptides that enable detection of TG activity, we investigated the expression and the activation pattern of each isozyme during differentiation in this culture system. In the differentiating cells, the protein levels, enzymatic activities, as well as localization of TG1 and TG3 exhibited distinct patterns. Specific knockdown of these enzymes by siRNA revealed less cornification, suggesting that each TG contributes to the epidermal formation. In conclusion, we demonstrate the efficiency of the 3D system for studying differentiation-dependent expression and activity of distinct TGs by specific substrate peptides. ENZYME: Transglutaminase, EC2.3.2.13.

DOI： 10.1111/febs.14832

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PubMed

Detection and identification of potential transglutaminase 2 substrates in the mouse renal glomeruli. Reviewed International journal

Yoshimasa Ito, Hideki Tatsukawa, Hisateru Yamaguchi, Kazuo Takahashi, Kiyotaka Hitomi, Yukio Yuzawa

Archives of biochemistry and biophysics Vol. 660 page： 11 - 19 2018.12

Language：English Publishing type：Research paper (scientific journal)

The glomerulus primarily comprises mesangial cells, glomerular microvascular endothelial cells, and podocytes. IgA nephropathy is the most common primary glomerulonephritis worldwide and has a risk of progression to end-stage renal disease. IgA nephropathy is characterized by predominant IgA deposition in the glomerular mesangial area, where TG2 is significantly enhanced. Therefore, identification of glomerular TG2 substrates is the first step in elucidating the role of TG2 as a crosslinking enzyme during disease progression. To clarify potential glomerular TG2 substrates, and to establish a procedure for substrate identification, we attempted to identify those molecules using normal mouse glomeruli. Extracts from mouse glomerular and non-glomerular fractions were treated with our established biotin-labeled substrate peptide, which specifically crosslinks to the lysine-donor substrates depending on TG2 activity. Peptide-incorporated proteins were then purified using avidin resin and identified via mass spectrometry. In parallel, we performed the identification using corresponding samples from TG2 knockout mice. Consequently, potential TG2 substrates were separately identified in glomerular and non-glomerular fractions. They were mainly identified as novel TG2 substrates and partly include the well-known substrates. These results potentially provide novel insights into the mechanism underlying IgA nephropathy and may help elucidate the physiological functions of TG2.

DOI： 10.1016/j.abb.2018.10.001

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PubMed

ISOZYME-SPECIFIC IDENTIFICATION AND CHARACTERIZATION OF SUBSTRATES CROSSLINKED BY TRANSGLUTAMINASES IN LIVER FIBROSIS

Tatsukawa H., Nakagawa H., Hitomi K.

ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH Vol. 42 page： 33A - 33A 2018.8

Tschimganine and its derivatives extend the chronological life span of yeast via activation of the Sty1 pathway. Reviewed International journal

Takahide Hibi, Hokuto Ohtsuka, Takafumi Shimasaki, Shougo Inui, Masatoshi Shibuya, Hideki Tatsukawa, Kei Kanie, Yoshihiko Yamamoto, Hirofumi Aiba

Genes to cells : devoted to molecular & cellular mechanisms Vol. 23 ( 8 ) page： 620 - 637 2018.8

Language：English Publishing type：Research paper (scientific journal)

Most antiaging factors or life span extenders are associated with calorie restriction (CR). Very few of these factors function independently of, or additively with, CR. In this study, we focused on tschimganine, a compound that was reported to extend chronological life span (CLS). Although tschimganine led to the extension of CLS, it also inhibited yeast cell growth. We acquired a Schizosaccharomyces pombe mutant with a tolerance for tschimganine due to the gene crm1. The resulting Crm1 protein appears to export the stress-activated protein kinase Sty1 from the nucleus to the cytosol even under stressful conditions. Furthermore, we synthesized two derivative compounds of tschimganine, α-hibitakanine and β-hibitakanine; these derivatives did not inhibit cell growth, as seen with tschimganine. α-hibitakanine extended the CLS, not only in S. pombe but also in Saccharomyces cerevisiae, indicating the possibility that life span regulation by tschimganine derivative may be conserved across various yeast species. We found that the longevity induced by tschimganine was dependent on the Sty1 pathway. Based on our results, we propose that tschimganine and its derivatives extend CLS by activating the Sty1 pathway in fission yeast, and CR extends CLS via two distinct pathways, one Sty1-dependent and the other Sty1-independent. These findings provide the potential for creating an additive life span extension effect when combined with CR, as well as a better understanding of the mechanism of CLS.

DOI： 10.1111/gtc.12604

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PubMed

Targeting transglutaminase 2 partially restores extracellular matrix structure but not alveolar architecture in experimental bronchopulmonary dysplasia. Reviewed International journal

Ivana Mižíková, Tilman Pfeffer, Claudio Nardiello, David E Surate Solaligue, Heiko Steenbock, Hideki Tatsukawa, Diogo M Silva, István Vadász, Susanne Herold, Richard J Pease, Siiri E Iismaa, Kiyotaka Hitomi, Werner Seeger, Jürgen Brinckmann, Rory E Morty

The FEBS journal Vol. 285 ( 16 ) page： 3056 - 3076 2018.8

Language：English Publishing type：Research paper (scientific journal)

The generation, maturation and remodelling of the extracellular matrix (ECM) are essential for the formation of alveoli during lung development. Alveoli formation is disturbed in preterm infants that develop bronchopulmonary dysplasia (BPD), where collagen fibres are malformed, and perturbations to lung ECM structures may underlie BPD pathogenesis. Malformed ECM structures might result from abnormal protein cross-linking, in part attributable to the increased expression and activity of transglutaminase 2 (TGM2) that have been noted in affected patient lungs, as well as in hyperoxia-based BPD animal models. The objective of the present study was to assess whether TGM2 plays a causal role in normal and aberrant lung alveolarization. Targeted deletion of Tgm2 in C57BL/6J mice increased septal thickness and reduced gas-exchange surface area in otherwise normally developing lungs. During aberrant lung alveolarization that occurred under hyperoxic conditions, collagen structures in Tgm2-/- mice were partially protected from the impact of hyperoxia, where normal dihydroxylysinonorleucine and hydroxylysylpiridinoline collagen cross-link abundance was restored; however, the lung alveolar architecture remained abnormal. Inhibition of transglutaminases (including TGM2) with cysteamine appreciably reduced transglutaminase activity in vivo, as assessed by Nε -(γ-l-glutamyl)-l-lysine abundance and TGM catalytic activity, and restored normal dihydroxylysinonorleucine and hydroxylysylpiridinoline collagen cross-link abundance under pathological conditions. Furthermore, a moderate improvement in alveoli size and gas-exchange surface density was noted in cysteamine-treated mouse lungs in which BPD was modelled. These data indicate that TGM2 plays a role in normal lung alveolarization, and contributes to the formation of aberrant ECM structures during disordered lung alveolarization.

DOI： 10.1111/febs.14596

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PubMed

Prevention of hepatocellular carcinoma by targeting MYCN-positive liver cancer stem cells with acyclic retinoid. Reviewed International journal

Xian-Yang Qin, Harukazu Suzuki, Masao Honda, Hikari Okada, Shuichi Kaneko, Ikuyo Inoue, Etsuko Ebisui, Kosuke Hashimoto, Piero Carninci, Keita Kanki, Hideki Tatsukawa, Naoto Ishibashi, Takahiro Masaki, Tomokazu Matsuura, Hiroyuki Kagechika, Kan Toriguchi, Etsuro Hatano, Yohei Shirakami, Goshi Shiota, Masahito Shimizu, Hisataka Moriwaki, Soichi Kojima

Proceedings of the National Academy of Sciences of the United States of America Vol. 115 ( 19 ) page： 4969 - 4974 2018.5

Language：English Publishing type：Research paper (scientific journal)

Hepatocellular carcinoma (HCC) is a highly lethal cancer that has a high rate of recurrence, in part because of cancer stem cell (CSC)-dependent field cancerization. Acyclic retinoid (ACR) is a synthetic vitamin A-like compound capable of preventing the recurrence of HCC. Here, we performed a genome-wide transcriptome screen and showed that ACR selectively suppressed the expression of MYCN, a member of the MYC family of basic helix-loop-helix-zipper transcription factors, in HCC cell cultures, animal models, and liver biopsies obtained from HCC patients. MYCN expression in human HCC was correlated positively with both CSC and Wnt/β-catenin signaling markers but negatively with mature hepatocyte markers. Functional analysis showed repressed cell-cycle progression, proliferation, and colony formation, activated caspase-8, and induced cell death in HCC cells following silencing of MYCN expression. High-content single-cell imaging analysis and flow cytometric analysis identified a MYCN+ CSC subpopulation in the heterogeneous HCC cell cultures and showed that these cells were selectively killed by ACR. Particularly, EpCAM+ cells isolated using a cell-sorting system showed increased MYCN expression and sensitivity to ACR compared with EpCAM- cells. In a long-term (>10 y) follow-up study of 102 patients with HCC, MYCN was expressed at higher levels in the HCC tumor region than in nontumor regions, and there was a positive correlation between MYCN expression and recurrence of de novo HCC but not metastatic HCC after curative treatment. In summary, these results suggest that MYCN serves as a prognostic biomarker and therapeutic target of ACR for liver CSCs in de novo HCC.

DOI： 10.1073/pnas.1802279115

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PubMed

糖尿病モデルマウスにおけるキヌレニン経路代謝物の腎組織イメージング質量分析

松下祥子, 高橋和男, 伊藤辰将, 山本康子, 藤垣英嗣, 毛利彰宏, 中嶋和紀, 尾之内高慶, 辰川英樹, 釘田雅則, 長尾静子, 人見清隆, 齋藤邦明, 瀬藤光利, 湯澤由紀夫

日本腎臓学会誌 Vol. 60 ( 3 ) page： 403 - 403 2018.4

組織の線維化に伴い架橋される基質タンパク質群の網羅的同定・解析 Invited

辰川英樹, 人見清隆

日本応用酵素協会 Vol. 52 page： 1-10 2018.3

組織の線維化に伴い架橋される基質タンパク質群の網羅的同定・解析 Invited

辰川英樹, 人見清隆

日本応用酵素協会 Vol. 52 page： 1-10 2018.3

Higher susceptibility to osmolality of the medaka (Oryzias latipes) mutants in orthologue genes of mammalian skin transglutaminases. Reviewed International journal

Yuko Watanabe, Eri Furukawa, Hideki Tatsukawa, Hisashi Hashimoto, Yasuhiro Kamei, Yoshihito Taniguchi, Kiyotaka Hitomi

Bioscience, biotechnology, and biochemistry Vol. 82 ( 7 ) page： 1165 - 1168 2018

Language：English Publishing type：Research paper (scientific journal)

Transglutaminase (TG) is an essential enzyme to catalyze cross-linking reactions of epidermal proteins. Recently, we biochemically characterized human skin TG orthologues for medaka (Oryzias latipes), a model fish. By genome editing, gene-modified fishes for the two orthologues were obtained, both of which lack the ordinal enzymes. These fish appeared to exhibit higher susceptibility to osmolality at the period of larvae.

DOI： 10.1080/09168451.2018.1453294

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PubMed

Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes. Reviewed International journal

Xian-Yang Qin, Mitsuko Hara, Erik Arner, Yoshikuni Kawaguchi, Ikuyo Inoue, Hideki Tatsukawa, Yutaka Furutani, Keisuke Nagatsuma, Tomokazu Matsuura, Feifei Wei, Jun Kikuchi, Hideko Sone, Carsten Daub, Hideya Kawaji, Timo Lassmann, Masayoshi Itoh, Harukazu Suzuki, Piero Carninci, Yoshihide Hayashizaki, Norihiro Kokudo, Alistair R R Forrest, Soichi Kojima

EBioMedicine Vol. 24 page： 257 - 266 2017.10

Language：English Publishing type：Research paper (scientific journal)

The acute phase protein orosomucoid-1 (Orm1) is mainly expressed by hepatocytes (HPCs) under stress conditions. However, its specific function is not fully understood. Here, we report a role of Orm1 as an executer of HPC proliferation. Increases in serum levels of Orm1 were observed in patients after surgical resection for liver cancer and in mice undergone partial hepatectomy (PH). Transcriptome study showed that Orm1 became the most abundant in HPCs isolated from regenerating mouse liver tissues after PH. Both in vitro and in vivo siRNA-induced knockdown of Orm1 suppressed proliferation of mouse regenerating HPCs and human hepatic cells. Microarray analysis in regenerating mouse livers revealed that the signaling pathways controlling chromatin replication, especially the minichromosome maintenance protein complex genes were uniformly down-regulated following Orm1 knockdown. These data suggest that Orm1 is induced in response to hepatic injury and executes liver regeneration by activating cell cycle progression in HPCs.

DOI： 10.1016/j.ebiom.2017.09.008

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PubMed

Global identification and analysis of isozyme-specific substrates crosslinked by transglutaminases in mouse liver fibrosis

Hideki Tatsukawa, Haruka Nakagawa, Hitomi Kiyotaka

HEPATOLOGY Vol. 66 page： 222A - 222A 2017.10

Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant. Reviewed International journal

Rima Horimizu, Ryota Ogawa, Yuko Watanabe, Hideki Tatsukawa, Masato Kinoshita, Hisashi Hashimoto, Kiyotaka Hitomi

The FEBS journal Vol. 284 ( 17 ) page： 2843 - 2855 2017.9

Language：English Publishing type：Research paper (scientific journal)

In the final process of blood coagulation, fibrin molecules are stabilized via a catalytic reaction by Factor XIIIA (FXIIIA), a member of the transglutaminase (TGase) family that catalyzes protein cross-linking reactions. In this study, we characterized the orthologue of this enzyme in medaka (Oryzias latipes), an established model fish in which a coagulation system is also preserved. The recombinant protein of this orthologue enzyme was produced in baculovirus-infected insect cells and used for analysis of its biochemical properties including activation by thrombin proteolysis and calcium dependence of the TGase enzymatic activity. Immunostaining and immunoblotting revealed that medaka FXIIIA is expressed in the kidney, bone, and esophagus in addition to blood cells. Furthermore, a gene-mutant fish was established using the CRISPR/Cas9 system. The loss of FXIIIA expression was validated in the mutants, and phenotypes, such as absence of fibrin cross-linking, were investigated in the established mutant fish.

DOI： 10.1111/febs.14153

Web of Science

PubMed

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Papers - TATSUKAWA Hideki