Updated on 2022/04/12

写真a

 
INOUE Shin-ichiro
 
Organization
Graduate School of Science Lecturer
Graduate School
Graduate School of Science
Undergraduate School
School of Science Department of Biological Science
Title
Lecturer
Contact information
メールアドレス

Degree 2

  1. 博士(理学) ( 2007.3   九州大学 ) 

  2. 修士(理学) ( 2005.3   九州大学 ) 

Research Interests 5

  1. ion transport

  2. Stomatal opening

  3. Blue light

  4. 植物科学

  5. 膨圧運動

Research Areas 2

  1. Life Science / Plant nutrition and soil science  / 多量栄養素、液胞

  2. Life Science / Plant molecular biology and physiology  / 光受容体、青色光、膨圧運動

Current Research Project and SDGs 3

  1. 青色光に依存した気孔開口を仲介するプロテインキナーゼの同定と機能的特徴づけ

  2. マメ科植物の葉の運動機構の解明

  3. 気孔開口を駆動する細胞膜H+-ATPaseの活性化機構の解明

Research History 1

  1. Nagoya University   Graduate School of Science Division of Biological Science Biological Systems   Lecturer

    2012.9

Education 2

  1. Kyushu University   Graduate School, Division of Natural Science

    2004.4 - 2007.3

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    Country: Japan

  2. Kyushu University   Faculty of Science

    1998.4 - 2002.3

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    Country: Japan

Professional Memberships 4

  1. 日本土壌肥料学会

    2020.4

  2. 日本光生物学協会

    2016.4

  3. 日本植物生理学会

  4. 日本植物学会

Awards 2

  1. 日本光生物学協会 奨励賞

    2018.8   青色光受容体フォトトロピンによる気孔開口調節メカニズムの研究

  2. 文部科学省 科学技術分野の文部科学大臣表彰 若手科学者賞

    2018.4   植物の青色光に対する応答の分子生物学的研究

 

Papers 33

  1. Type 2C protein phosphatase clade D family members dephosphorylate guard cell plasma membrane H+-ATPase Reviewed International coauthorship

    Akiyama Mitsumasa, Sugimoto Hodaka, Inoue Shin-ichiro, Takahashi Yohei, Hayashi Maki, Hayashi Yuki, Mizutani Miya, Ogawa Takumi, Kinoshita Daichi, Ando Eigo, Park Meeyeon, Gray William M., Kinoshita Toshinori

    PLANT PHYSIOLOGY     2021.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/plphys/kiab571

    Web of Science

  2. Raf-like kinases CBC1 and CBC2 negatively regulate stomatal opening by negatively regulating plasma membrane H+-ATPase phosphorylation. Reviewed

    Hayashi M, Sugimoto H, Takahashi H, Seki M, Shinozaki K, Sawasaki T, Kinoshita T, Inoue S

    Photochemical & Photobiological Sciences   Vol. 19   page: 88 - 98   2020

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  3. CIPK23 regulates blue light-dependent stomatal opening in Arabidopsis thaliana. Reviewed International coauthorship

    Inoue S, Kaiserli E, Zhao X, Waksman T, Takemiya A, Okumura M, Takahashi H, Seki M, Shinozaki K, Endo Y, Sawasaki T, Kinoshita T, Zhang X, Christie JM, Shimazaki K

    The Plant J   Vol. 104   page: 679 - 692   2020

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  4. Stomatal response to blue light in crassulacean acid metabolism plants Kalanchoe pinnata and Kalanchoe daigremontiana. Reviewed

    Gotoh E, Oiwamoto K, Inoue S, Shimazaki K, Doi M

    Journal of Experimental Botany   ( 70 ) page: 1367 - 1374   2019

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  5. A Raf-like protein kinase BHP mediates blue light-dependent stomatal opening Reviewed

    Hayashi M, Inoue S, UenoY, Kinoshita T

    Scientific Reports   Vol. 7   page: 45586   2017

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    Language:English   Publishing type:Research paper (scientific journal)  

  6. Blue light regulation of stomatal opening and the plasma membrane H+-ATPase Invited Reviewed

    Inoue S, Kinoshita T

    Plant Physiology   Vol. 174   page: 531-538   2017

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    Authorship:Lead author   Language:English  

  7. Brassinosteroid involvement in Arabidopsis thaliana stomatal opening Reviewed

    Inoue S, Iwashita N, Takahashi Y, Gotoh E, Okuma E, Hayashi M, Tabata R, Takemiya A, Murata Y, Doi M, Kinoshita T, Shimazaki K

    Plant & Cell Physiology   Vol. 58   page: 1048-1058   2017

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  8. Photosynthesis activates plasma membrane H+-ATPase via sugar accumulation. Reviewed

    Okumura M, Inoue S, Kuwata K, Kinoshita T

    Plant Physiology   Vol. 171   page: 580-589   2016.3

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    Language:English   Publishing type:Research paper (scientific journal)  

  9. GOLDEN 2-LIKE transcription factors for chloroplast development affect ozone tolerance through the regulation of stomatal movement. Reviewed

    Nagatoshi Y, Mitsuda N, Hayashi M, Inoue S, Okuma E, Kubo A, Murata Y, Seo M, Saji H, Kinoshita T, Ohme-Takagi M

    Proc. Natl. Acad. Sci. USA   Vol. 113   page: 4218-4223   2016

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    Language:English   Publishing type:Research paper (scientific journal)  

  10. Overexpression of plasma membrane H+-ATPase in guard cells promotes light-induced stomatal opening and enhances plant growth. Reviewed

    Wang Y, Noguchi K, Ono N, Inoue S, Terashima I, Kinoshita T

    Proc. Natl. Acad. Sci. USA   Vol. 111   page: 533-538   2014

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    Language:English   Publishing type:Research paper (scientific journal)  

  11. Characterization of the plasma membrane H+-ATPase in the liverwort Marchantia polymorpha. Reviewed

    Okumura M, Inoue S, Takahashi K, Ishizaki K, Kohchi T, Kinoshita T

    Plant Physiology   Vol. 159   page: 826-834   2012

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    Language:English   Publishing type:Research paper (scientific journal)  

  12. Functional analyses of the activation loop of phototropin2 in Arabidopsis. Reviewed

    Inoue S, Matsushita T, Tomokiyo Y, Matsumoto M, Nakayama KI, Kinoshita T, Shimazaki K

    Plant Physiology   Vol. 156   page: 1391-1405   2011

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  13. Immunohistochemical detection of blue light-induced phosphorylation of the plasma membrane H+-ATPase in stomatal guard cells. Reviewed

    Hayashi M, Inoue S, Takahashi K, Kinoshita T

    Plant & Cell Physiology   Vol. 52   page: 1238-1248   2011

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    Language:English   Publishing type:Research paper (scientific journal)  

  14. Phototropin signaling and stomatal opening as a model case. Invited Reviewed

    Inoue S, Takemiya A, Shimazaki K

    Current Opinion in Plant Biology   Vol. 13   page: 587-593   2010

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  15. Blue light-induced autophosphorylation of phototropin is a primary step for signaling. Reviewed

    Inoue S, Kinoshita T, Matsumoto M, Nakayama KI, Doi M, Shimazaki K

    Proc Natl Acad Sci USA   Vol. 105   page: 5626-5631   2008

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  16. Leaf positioning of Arabidopsis in response to blue light. Reviewed

    Inoue S, Kinoshita T, Takemiya A, Doi M, Shimazaki K

    Molecular Plant   Vol. 1   page: 15-26   2008

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  17. Possible involvement of phototropins in leaf movement of kidney bean in response to blue light. Reviewed

    Inoue S, Kinoshita T, Shimazaki K

    Plant Physiology   Vol. 138   page: 1994-2004   2005

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  18. Phototropins promote plant growth in response to blue light in low light environments. Reviewed

    Takemiya A*, Inoue S*, Doi M*, Kinoshita T, Shimazaki K

    The Plant Cell   Vol. 17   page: 1120-1127   2005

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  19. Phototropin2 contributes to the chloroplast avoidance response at the chloroplast-plasma membrane interface. Reviewed

    Ishishita K, Higa T, Tanaka H, Inoue S, Chung A, Ushijima T, Matsushita T, Kinoshita T, Nakai M, Wada M, Suetsugu N, Gotoh E

    Plant Physiology   Vol. 183   page: 304 - 316   2020

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    Language:English   Publishing type:Research paper (scientific journal)  

  20. Excess pyrophosphate within guard cells delays stomatal closure. Reviewed

    Asaoka M, Inoue S, Gunji S, Kinoshita T, Maeshima M

    Plant & Cell Physiology   Vol. 60   page: 875 - 887   2019

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    Language:English   Publishing type:Research paper (scientific journal)  

  21. Brassinosteroid induces phosphorylation of the plasma membrane H+-ATPase during hypocotyl elongation in Arabidopsis thaliana. Reviewed

    Minami A, Takahashi K, Inoue S, Tada Y, Kinoshita T

    Plant & Cell Physiology   Vol. 60   page: 935 - 944   2019

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    Language:English   Publishing type:Research paper (scientific journal)  

  22. Functional characterization of a constitutively active kinase variant of Arabidopsis phototropin 1 Reviewed International coauthorship

    Petersen J, Inoue S, Kelly SM, Sullivan S, Kinoshita T, Christie JM

    Journal of Biological Chemistry   Vol. 292   page: 13843-13852   2017

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    Language:English   Publishing type:Research paper (scientific journal)  

  23. Auxin influx carrier AUX1 confers acid resistance for Arabidopsis root elongation through the regulation of plasma membrane H+-ATPase. Reviewed

    Inoue S, Takahashi K, Okumura-Noda H, Kinoshita T

    Plant & Cell Physiology   Vol. 57   page: 2194-2201   2016.8

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  24. A flowering integrator, SOC1, affects stomatal opening in Arabidopsis thaliana. Invited Reviewed

    Kimura Y, Aoki S, Ando E, Kitatsuji A, Watanabe A, Ohnishi M, Takahashi K, Inoue S, Nakamichi N, Tamada Y, Kinoshita T

    Plant & Cell Physiology   Vol. 56 ( 4 ) page: 640-649   2015.1

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    Language:English   Publishing type:Research paper (scientific journal)  

  25. Mg-chelatase I subunit 1 and Mg-Protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana. Reviewed

    Tomiyama M, Inoue S, Tsuzuki T, Soda M, Morimoto S, Okigaki Y, Ohishi T, Mochizuki N, Takahashi K, Kinoshita T

    Journal of Plant Research   Vol. 127   page: 553-563   2014

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    Language:English   Publishing type:Research paper (scientific journal)  

  26. Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells. Reviewed

    Sakai Y, Inoue S, Harada A, Shimazaki K, Takagi S

    Journal of Integrative Plant Biology     page: In press   2014

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    Language:English   Publishing type:Research paper (scientific journal)  

  27. Role of RPT2 in leaf positioning and flattening under various light environments and a possible inhibition of phot2 signaling by phot1 Reviewed

    Harada A, Takemiya A, Inoue S, Sakai T, Shimazaki K

    Plant & Cell Physiology   Vol. 54   page: 36-47   2013

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    Language:English   Publishing type:Research paper (scientific journal)  

  28. TWIN SISTER OF FT, GIGANTEA, and CONSTANS have a positive but indirect effect on blue light-induced stomatal opening in Arabidopsis Reviewed

    1. Ando E, Ohnishi M, Wang Y, Matsushita T, Watanabe A, Hayashi Y, Fujii M, Ma JF, Inoue S, Kinoshita T

    Plant Physiology   Vol. 162   page: 1529-1538   2013

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    Language:English   Publishing type:Research paper (scientific journal)  

  29. Evolutionary appearance of the plasma membrane H+-ATPase containing a penultimate threonine in the bryophyte. Invited Reviewed

    Okumura M, Takahashi K, Inoue S, Kinoshita T

    Plant Signaling & Behavior   Vol. 7   page: 979-982   2012

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    Language:English   Publishing type:Research paper (scientific journal)  

  30. Mg-chelatase H subunit affects ABA signaling in stomatal guard cells, but is not an ABA receptor in Arabidopsis thaliana. Reviewed

    Tsuzuki T, Takahashi K, Inoue S, Okigaki Y, Tomiyama M, Hossain MA, Shimazaki K, Murata Y, Kinoshita T

    Journal of Plant Research   Vol. 124   page: 527-538   2011

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    Language:English   Publishing type:Research paper (scientific journal)  

  31. FLOWERING LOCUS T regulates stomatal opening. Reviewed

    Kinoshita T, Ono N, Hayashi Y, Morimoto S, Nakamura S, Soda M, Kato Y, Ohnishi M, Nakano T, Inoue S, Shimazaki K

    Current Biology   Vol. 21   page: 1232-1238   2011

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    Language:English   Publishing type:Research paper (scientific journal)  

  32. The Arabidopsis PHYTOCHROME KINASE SUBSTRATE2 protein is a phototropin signaling element that regulates leaf flattening and leaf positioning. Reviewed International coauthorship

    de Carbonnel M, Davis P, Roelfsema MRG, Inoue S, Schepens I, Lariguet P, Geisler M, Shimazaki K, Hangarter R, Fankhauser C

    Plant Physiology   Vol. 152   page: 1391-1405   2010

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    Language:English   Publishing type:Research paper (scientific journal)  

  33. Biochemical characterization of the plasma membrane H+-ATPase activation in guard-cell protoplasts of Arabidopsis thaliana in response to blue light. Reviewed

    Ueno K, Kinoshita T, Inoue S, Emi T, Shimazaki K

    Plant & Cell Physiology   Vol. 46   page: 955-963   2005

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    Language:English   Publishing type:Research paper (scientific journal)  

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Books 3

  1. 植物の気孔開口を誘導する青色光シグナル伝達の解明

    井上晋一郎( Role: Contributor)

    アグリバイオ(北隆館)  2021 

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    Responsible for pages:266-271   Language:Japanese Book type:Scholarly book

  2. 気孔開口に必要なカリウム取込みを調節する新たなシグナル伝達の解明

    井上晋一郎( Role: Contributor)

    アグリバイオ(北隆館)  2021 

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    Responsible for pages:476-481   Language:Japanese Book type:Scholarly book

  3. 植物の気孔開口を誘導する青色光シグナル伝達の解明

    井上晋一郎( Role: Sole author)

    アグリバイオ(北隆館)年末増刊号  2021.11 

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    Language:Japanese Book type:Textbook, survey, introduction

Presentations 2

  1. 新奇液胞膜型マグネシウム輸送体の気孔開口への関与

    井上 晋一郎、林 真妃、黄 勝、横正 健剛、後藤 栄治、池松 朱夏、奥村 将樹、鈴木 孝征、木下 俊則、馬 建鋒

    日本植物生理学会年会、つくば大会  2022.3 

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    Event date: 2022.3

    Language:English   Presentation type:Oral presentation (general)  

  2. 植物のMgホメオスタシス維持を調節するシグナル伝達機構の解析

    井上晋一郎、横正健剛、吉成晃、林真妃、後藤栄治、馬建鋒、木下俊則

    日本土壌肥料学会年会、北海道大会  2021.9 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

KAKENHI (Grants-in-Aid for Scientific Research) 2

  1. 気孔の開口を駆動する細胞膜プロトンポンプの活性調節機構の解明

    Grant number:20K06703  2020.4 - 2023.3

    科学研究費助成事業  基盤研究(C)

    井上 晋一郎

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    Authorship:Principal investigator 

    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

    植物体表には、気孔とよばれる穴が無数に存在し、植物は気孔を介して光合成に必要なCO2を体内に取込む。気孔は一対の孔辺細胞に囲まれて形成され、青色光に応答して開口する。気孔が開口するには、孔辺細胞の細胞膜プロトンポンプが活性化される必要があるが、青色光がどのようにプロトンポンプを活性化するのか、その分子メカニズムが未解明である。本研究では、私達がこれまでの研究で同定していた2つの気孔開口調節因子の機能解析を通じて、孔辺細胞のプロトンポンプの活性化メカニズムを分子遺伝学的に明らかにする。この研究を通じて、気孔が太陽光のもとどのように開口し、植物の光合成と生長に貢献しているのか明らかにする。
    植物の表皮に存在する気孔は青色光に応答して開口し、光合成を支える重要なガス交換を担う。青色光受容体フォトトロピンは、気孔孔辺細胞において細胞膜H+-ATPaseを活性化して細胞膜を過分極させ、電位依存性のカリウムチャネルを介したカリウム取込みを駆動して気孔を開口させる。ところが、青色光がどのようにこれらのイオン輸送体を活性化するのか、気孔開口機構の分子メカニズムは完全には明らかになっていない。これまでに申請者は、フォトトロピンと相互作用するプロテインキナーゼ(PINKs)を多数同定しており、本研究では気孔開口におけるPINKsの生理・生化学的機能を明らかにすることで気孔開口機構をより理解することを目的としている。
    本年度は、主にPINK1とPINK2に関して研究を行った。PINK1はCIPK23であり、T-DNA挿入変異体を用いた表現型解析から、気孔開口を正に調節することが明らかになった。単離孔辺細胞を用いた解析から、cipk23変異体では青色光による細胞膜H+-ATPaseの活性化は正常に観察されたものの、カリウムチャネルの活性化が抑制されていた。パッチクランプ法による電気生理学実験から、青色光がカリウムチャネル活性を細胞膜H+-ATPaseの活性とは無関係に促進することを明らかにし、このチャネル活性の促進をCIPK23が担うことを明らかにした。以上の結果から、青色光による気孔開口において、細胞膜H+-ATPaseとは別のカリウムチャネル活性化シグナル伝達経路を見出した。
    また、PINK2に関しては、フォトトロピンに加えて細胞膜H+-ATPaseとも相互作用することを明らかにし、PINK2がH+-ATPaseを直接リン酸化して活性化する可能性を見出した。
    PINK1(CIPK23)の機能解析を完了し、気孔開口における新たなシグナル伝達経路を明らかにすることができたため。
    2021年度は主にPINK2の機能解析を行う。PINK2が細胞膜H+-ATPaseを直接リン酸化するのか、in vitroキナーゼアッセイを行うほか、酵母を用いた異生物発現系を用いた実験を進めて検証する。また、現在PINK2をコードするプロテインキナーゼの多重変異体を作成しているため、出来次第気孔開度と気孔コンダクタンスの測定を進める。さらに、孔辺細胞H+-ATPaseのリン酸化が野生株に比べて低くなるのか、生理・生化学実験により検証する。以上の解析を行い、PINK2がフォトトロピンの下流、かつ細胞膜H+-ATPaseの上流で機能し、気孔開口を仲介する新規シグナル伝達因子である可能性を検証する。

  2. Identification and functional characterization of protein kinases mediating blue light-dependent stomatal opening

    Grant number:15K07101  2015.4 - 2018.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Inoue Shin-ichiro, HAYASHI Maki, OKUMURA Masaki

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    Authorship:Principal investigator 

    Grant amount:\5200000 ( Direct Cost: \4000000 、 Indirect Cost:\1200000 )

    Stomata open in response to blue light, and the opening is mediated by a phototropin-activated proton pump and a consequent potassium uptake in stomatal guard cells. However, signaling for the stomatal opening is not fully understood. In this study, we performed a protein-protein interaction screening and identified CIPK23 as a phototropin interactor. As the results of functional analyses of CIPK23, we found that CIPK23 mediates stomatal opening not through the proton pump activation but through the potassium channel activation in guard cells.

 

Teaching Experience (On-campus) 4

  1. 基礎遺伝学III

    2021

  2. 生物学入門

    2021

  3. 専門実習

    2021

  4. 基礎遺伝学III

    2019