研究者詳細 - 堀　克敏

論文 - 堀　克敏

分割表示 148 件中 61 - 80 件目／全件表示 >>

Structural basis for toughness and flexibility in the C-terminal Passenger domain of an Acinetobactor trimeric autotranspoter Adhesin 査読有り

K. Koiwai, M.D.Hartmann, D. Linke, A.N. Lupas and K.Hori

J. Biol. Chem. 291 巻頁： 3705-3724 2016年2月

Effectiveness of direct immobilization of bacterial cells onto material surfaces using the bacterionanofiber protein AtaA. 査読有り

Hori K, Ohara Y, Ishikawa M, Nakatani H.

Applied Microbiology and Biotechnology 2015年4月

記述言語：英語掲載種別：研究論文（学術雑誌）

The bacterionanofiber protein AtaA, a member of the trimeric autotransporter adhesin family found in Acinetobacter sp. Tol 5, is responsible for the nonspecific, high adhesiveness and autoagglutination of this strain. Previously, we introduced the ataA gene into the nonadhesive Acinetobacter strain ST-550, which conferred high adhesiveness to this strain, immobilized its cells, and improved indigo productivity due to enhanced tolerance to the toxic substrate. In this study, we again demonstrated the effectiveness of this new microbial immobilization method using AtaA in a number of conditions. AtaA enabled the effective immobilization of growing, resting, and lyophilized cells of a type strain of Acinetobacter, ADP1, which is also intrinsically nonadhesive, onto the surface of several kinds of support ranging from artificial to natural materials and from hydrophobic polyurethane to hydrophilic glass. Immobilization with AtaA enabled exclusive cell growth in the support space and only a few cells existed in the bulk medium. Immobilization of resting cells drastically increased cell concentration, depending on the support material; dry cells of approximately 110 g/L could be immobilized onto glass wool. Finally, we demonstrated that ADP1 cells immobilized on polyurethane foam can undergo at least 10 repetitive reactions without inactivation during a 5-h period. Even after drying and storing for 3 days, the immobilized cells showed enzymatic activity and an ester hydrolysis reaction was repeated by simply transferring the support with the cells into a fresh reaction buffer.

DOI： 10.1007/s00253-015-6554-9

PubMed

Definition of the transcription factor TdIF1 consensus-binding sequence through genomewide mapping of its binding sites 査読有り

K. Koiwai,T. Kubota, N. Watanabe, K. Hori, O. Koiwai and H. Masai

Genes to Cells 20 巻 ( 3 ) 頁： 242-254 2015年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

TdIF1 was originally identified as a protein that directly binds to terminal deoxynucleotidyltransferase, TdT. Through in vitro selection assays (SELEX), we recently showed that TdIF1 recognizes both AT-tract and a specific DNA sequence motif, 5'-TGCATG-3', and can up-regulate the expression of RAB20 through the latter motif. However, whether TdIF1 binds to these sequences in the cells has not been clear and its other target genes remain to be identified. Here, we determined in vivo TdIF1-binding sequences (TdIF1-invivoBMs) on the human chromosomes through ChIP-seq analyses. The result showed a 160-base pair cassette containing 'AT-tract~palindrome (inverted repeat)~AT-tract' as a likely target sequence of TdIF1. Interestingly, the core sequence of the palindrome in the TdIF1-invivoBMs shares significant similarity to the above 5'-TGCATG-3' motif determined by SELEX in vitro. Furthermore, spacer sequences between AT-tract and the palindrome contain many potential transcription factor binding sites. In luciferase assays, TdIF1 can up-regulate transcription activity of the promoters containing the TdIF1-invivoBM, and this effect is mainly through the palindrome. Clusters of this motif were found in the potential target genes. Gene ontology analysis and RT-qPCR showed the enrichment of some candidate targets of TdIF1 among the genes involved in the regulation of ossification. Potential modes of transcription activation by TdIF1 are discussed.

DOI： 10.1111/gtc.12216

PubMed

バイオフィルムを利用した界面微生物工学

堀克敏

化学療法の領域 31 巻 ( 11 ) 頁： 88-96 2015年

Application of the adhesive bacterionanofiber AtaA to a novel microbial immobilization method for the production of indigo as a model chemical 査読有り

M. Ishikawa, K. Sugimori, K. Hori

Biotechnol. Bioeng. 111 巻頁： 16-24 2014年1月

TdiF1 recognizes a specific DNA sequence through its helix-turn-helix and AT-hook motifs to regulate gene transcription 査読有り

T. Kubota, O. Koiwai, K. Hori, N. Watanabe, K. Koiwai

PLoS ONE 8 巻頁： e66710 2013年

Extracellular electron transfer of a highly adhesive and metabolically versatile bacterium 査読有り

H. Liu, M. Ishikawa, S. Matsuda, Y. Kimoto, K. Hori, K. Hashimoto, S. Nakanishi

ChemPhysChem 14 巻頁： 2407-2412 2013年

A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination 査読有り

M. Ishikawa, K. Hori

BMC Microbiology 13 巻頁： 86 2013年

炭素繊維が微生物を集めて水を浄化するメカニズム

松本慎也，堀克敏

繊維機械学会誌 ( 66 ) 頁： 29-33 2013年

AtaA, a new member of the trimeric autotransporter adhesins from Acinetobacter sp. Tol 5 mediating high adhesiveness to various abiotic surfaces 査読有り

M. Ishikawa, H. Nakatani, K. Hori

PLoS ONE 7 巻頁： e48830 2012年11月

Carbon fiber as an Excellent Support Material for Wastewater Treatment Biofilms 査読有り

Shinya Matsumoto, Akihito Ohtaki, Katsutoshi Hori

Environmental Science & Technology 46 巻頁： 10175-10181 2012年9月

Evaluation of adhesiveness of Acinetobacter sp.Tol5 to abiotic surfaces 査読有り

M. Ishikawa, K. Shigemori, A. Suzuki, and K. Hori

J. Biosci. Bioeng. 113 巻頁： 719-725 2012年6月

バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築招待有り

堀　克敏

化学工学 76 巻 ( 4 ) 頁： 212-214 2012年4月

界面微生物工学によるグリーン・イノベーションを目指して招待有り

堀　克敏

化学工業 63 巻 ( 1 ) 頁： 70-74 2012年1月

微生物付着・バイオフィルムの制御と応用から界面微生物工学査読有り

堀　克敏

オレオサイエンス 11 巻頁： 19-24 2012年

バクテリオナノファイバーの細胞接着機能と応用招待有り

堀　克敏

高分子 60 巻 ( 10 ) 頁： 747-748 2011年6月

Production of peritrichate bacterionanofibers and their proteinaceous components by Acinetobacter sp. Tol 5 cell affected by growth substrates 査読有り

111 巻頁： 31-36 2011年

Simultaneous syntheses of polyhydroxyalkanoates and rhamnolipids by Pseudomonas aeruginosa IFO3924 at various temperatures and from various fatty acids 査読有り

K. Hori, R. Ichinohe, H. Unno, and S. Marsudi

Biochem. Eng. J 53 巻頁： 196-202 2011年

バクテリオナノファイバーによる細菌の細胞接着招待有り

堀　克敏

25 巻頁： 1-7 2011年

Effects of microbial degradation of biofoulants on microfiltration membrane performance in a membrane bioreactor 査読有り

D. Okamura, Y. Mori, T. Hashimoto, and K. Hori

Environ. Sci. Technol 44 巻頁： 8644-8648 2010年

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