Faculty Profiles - HORI Katsutoshi

Papers - HORI Katsutoshi

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バイオコントロールによる高濃度油脂含有排水のハイパフォーマンス処理 Invited Reviewed

堀　克敏

環境バイオテクノロジー学会誌 Vol. 17 page： 3-8 2017.3

Cell surface protein engineering for high-performance whole-cell catalysts Reviewed International journal

Nakatani Hajime, Hori Katsutoshi

FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING Vol. 11 ( 1 ) page： 46 - 57 2017.3

Language：English Publishing type：Research paper (scientific journal) Publisher：Frontiers of Chemical Science and Engineering

Cell surface protein engineering facilitated by accumulation of information on genome and protein structure involves heterologous production and modification of cell surface proteins using genetic engineering, and is important for the development of high-performance whole-cell catalysts. In this field, cell surface display is a major technology by exposing target proteins, such as enzymes, on the cell surface using a carrier protein. The target proteins are fused to the carrier proteins that transport and tether them to the cell surface, as well as to a secretion signal. This paper reviews cell surface display systems for prokaryotic and eukaryotic cells from the perspective of carrier proteins, which determine the number of displayed molecules, and the localization, size, and direction (N- or C-terminal anchoring) of the passengers. We also discuss advanced methods for displaying multiple enzymes and a new method for the immobilization of whole-cell catalysts using adhesive surface proteins.

DOI： 10.1007/s11705-017-1609-3

Web of Science

Scopus

バイオフィルム・微生物付着の工学利用 Open Access

堀　克敏

膜 Vol. 42 page： 54-59 2017

細胞表層タンパク質の合成生物学 Invited Open Access

石川聖人、堀　克敏

生物工学会誌 Vol. 94 page： 701-703 2016.11

細菌の持つ接着ナノファイバータンパク質AtaA

堀　克敏

バイオサイエンスとインダストリー Vol. 74 page： 380-381 2016.11

Secretion of the intimin passenger domain is driven by protein folding Reviewed Open Access

J. C. Leo, P. Oberhettinger, S. Yoshimoto, D. B. R. K. G. Udatha, J. P. Morth, M. Schütz, K. Hori, and D. Linke

J. Biol. Chem Vol. 291 page： 20096 2016.7

An Acinetobacter trimeric autotranspoter adhesin reaped from cells exhibits its nonspecific stickiness via a highly stable 3D strucuture Reviewed Open Access

S. Yoshimoto, H. Nakatani, K.Iwasaki and K.Hori

Sci Rep 2016.6

Discovery of a novel periplasmic protein that forms acomplex with a trimeric autotransporter adhesin and Peptidoglycan Reviewed Open Access

M. Ishikawa, S. Yoshimoto, A. Hayashi, J. Kanie and K.Hori

Mol Microbiol 2016.5

細菌接着タンパク質AtaA分子の特性と応用 Invited

堀　克敏

バイオサイエンスとインダストリー Vol. 74 page： 417-419 2016.5

Structural basis for toughness and flexibility in the C-terminal Passenger domain of an Acinetobactor trimeric autotranspoter Adhesin Reviewed Open Access

K. Koiwai, M.D.Hartmann, D. Linke, A.N. Lupas and K.Hori

J. Biol. Chem. Vol. 291 page： 3705-3724 2016.2

Effectiveness of direct immobilization of bacterial cells onto material surfaces using the bacterionanofiber protein AtaA. Reviewed

Hori K, Ohara Y, Ishikawa M, Nakatani H.

Applied Microbiology and Biotechnology 2015.4

Language：English Publishing type：Research paper (scientific journal)

The bacterionanofiber protein AtaA, a member of the trimeric autotransporter adhesin family found in Acinetobacter sp. Tol 5, is responsible for the nonspecific, high adhesiveness and autoagglutination of this strain. Previously, we introduced the ataA gene into the nonadhesive Acinetobacter strain ST-550, which conferred high adhesiveness to this strain, immobilized its cells, and improved indigo productivity due to enhanced tolerance to the toxic substrate. In this study, we again demonstrated the effectiveness of this new microbial immobilization method using AtaA in a number of conditions. AtaA enabled the effective immobilization of growing, resting, and lyophilized cells of a type strain of Acinetobacter, ADP1, which is also intrinsically nonadhesive, onto the surface of several kinds of support ranging from artificial to natural materials and from hydrophobic polyurethane to hydrophilic glass. Immobilization with AtaA enabled exclusive cell growth in the support space and only a few cells existed in the bulk medium. Immobilization of resting cells drastically increased cell concentration, depending on the support material; dry cells of approximately 110 g/L could be immobilized onto glass wool. Finally, we demonstrated that ADP1 cells immobilized on polyurethane foam can undergo at least 10 repetitive reactions without inactivation during a 5-h period. Even after drying and storing for 3 days, the immobilized cells showed enzymatic activity and an ester hydrolysis reaction was repeated by simply transferring the support with the cells into a fresh reaction buffer.

DOI： 10.1007/s00253-015-6554-9

PubMed

Definition of the transcription factor TdIF1 consensus-binding sequence through genomewide mapping of its binding sites Reviewed Open Access

K. Koiwai,T. Kubota, N. Watanabe, K. Hori, O. Koiwai and H. Masai

Genes to Cells Vol. 20 ( 3 ) page： 242-254 2015.1

Language：English Publishing type：Research paper (scientific journal)

TdIF1 was originally identified as a protein that directly binds to terminal deoxynucleotidyltransferase, TdT. Through in vitro selection assays (SELEX), we recently showed that TdIF1 recognizes both AT-tract and a specific DNA sequence motif, 5'-TGCATG-3', and can up-regulate the expression of RAB20 through the latter motif. However, whether TdIF1 binds to these sequences in the cells has not been clear and its other target genes remain to be identified. Here, we determined in vivo TdIF1-binding sequences (TdIF1-invivoBMs) on the human chromosomes through ChIP-seq analyses. The result showed a 160-base pair cassette containing 'AT-tract~palindrome (inverted repeat)~AT-tract' as a likely target sequence of TdIF1. Interestingly, the core sequence of the palindrome in the TdIF1-invivoBMs shares significant similarity to the above 5'-TGCATG-3' motif determined by SELEX in vitro. Furthermore, spacer sequences between AT-tract and the palindrome contain many potential transcription factor binding sites. In luciferase assays, TdIF1 can up-regulate transcription activity of the promoters containing the TdIF1-invivoBM, and this effect is mainly through the palindrome. Clusters of this motif were found in the potential target genes. Gene ontology analysis and RT-qPCR showed the enrichment of some candidate targets of TdIF1 among the genes involved in the regulation of ossification. Potential modes of transcription activation by TdIF1 are discussed.

DOI： 10.1111/gtc.12216

PubMed

バイオフィルムを利用した界面微生物工学

堀克敏

化学療法の領域 Vol. 31 ( 11 ) page： 88-96 2015

Application of the adhesive bacterionanofiber AtaA to a novel microbial immobilization method for the production of indigo as a model chemical Reviewed

M. Ishikawa, K. Sugimori, K. Hori

Biotechnol. Bioeng. Vol. 111 page： 16-24 2014.1

TdiF1 recognizes a specific DNA sequence through its helix-turn-helix and AT-hook motifs to regulate gene transcription Reviewed Open Access

T. Kubota, O. Koiwai, K. Hori, N. Watanabe, K. Koiwai

PLoS ONE Vol. 8 page： e66710 2013

Extracellular electron transfer of a highly adhesive and metabolically versatile bacterium Reviewed

H. Liu, M. Ishikawa, S. Matsuda, Y. Kimoto, K. Hori, K. Hashimoto, S. Nakanishi

ChemPhysChem Vol. 14 page： 2407-2412 2013

A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination Reviewed Open Access

M. Ishikawa, K. Hori

BMC Microbiology Vol. 13 page： 86 2013

炭素繊維が微生物を集めて水を浄化するメカニズム

松本慎也，堀克敏

繊維機械学会誌 ( 66 ) page： 29-33 2013

AtaA, a new member of the trimeric autotransporter adhesins from Acinetobacter sp. Tol 5 mediating high adhesiveness to various abiotic surfaces Reviewed Open Access

M. Ishikawa, H. Nakatani, K. Hori

PLoS ONE Vol. 7 page： e48830 2012.11

Carbon fiber as an Excellent Support Material for Wastewater Treatment Biofilms Reviewed

Shinya Matsumoto, Akihito Ohtaki, Katsutoshi Hori

Environmental Science & Technology Vol. 46 page： 10175-10181 2012.9

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Papers - HORI Katsutoshi