Updated on 2021/11/16

写真a

 
KUROTANI Ken-ichi
 
Organization
Bioscience and Biotechnology Center Designated lecturer
Title
Designated lecturer
External link

Degree 1

  1. 博士(農学) ( 2002.3   京都大学 ) 

Research Interests 4

  1. bio-informatics

  2. plant signal transduction

  3. environmental stress

  4. plant physiology

Research Areas 1

  1. Life Science / Plant molecular biology and physiology

Current Research Project and SDGs 1

  1. 接木の成立メカニズムの解明と異科接木の農業利用

Research History 1

  1. Nagoya University Bioscience and Biotechnology Center   Designated lecturer

Education 3

  1. Kyoto University

    1997.4 - 2001.3

  2. Kyoto University

    1995.4 - 1997.3

  3. Kyoto University

    1991.4 - 1995.3

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    Country: Japan

Committee Memberships 1

  1. 日本植物学会   電子出版物編集委員  

    2021.4   

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    Committee type:Academic society

 

Papers 19

  1. Cell-to-Cell Connection in Plant Grafting - Molecular Insights into Symplasmic Reconstruction.

    Kurotani KI, Notaguchi M

    Plant & cell physiology     2021.7

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    Language:English  

    DOI: 10.1093/pcp/pcab109

    PubMed

  2. Cell-cell adhesion in plant grafting is facilitated by beta-1,4-glucanases

    Notaguchi Michitaka, Kurotani Ken-ichi, Sato Yoshikatsu, Tabata Ryo, Kawakatsu Yaichi, Okayasu Koji, Sawai Yu, Okada Ryo, Asahina Masashi, Ichihashi Yasunori, Shirasu Ken, Suzuki Takamasa, Niwa Masaki, Higashiyama Tetsuya

    SCIENCE   Vol. 369 ( 6504 ) page: 698 - +   2020.8

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  3. Host-parasite tissue adhesion by a secreted type of beta-1,4-glucanase in the parasitic plant Phtheirospermum japonicum

    Kurotani Ken-ichi, Wakatake Takanori, Ichihashi Yasunori, Okayasu Koji, Yu Sawai, Ogawa Satoshi, Cui Songkui, Suzuki Takamasa, Shirasu Ken, Notaguchi Michitaka

    COMMUNICATIONS BIOLOGY   Vol. 3 ( 1 ) page: 407   2020.7

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    Publisher:Communications Biology  

    DOI: 10.1038/s42003-020-01143-5

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  4. Tissue adhesion between distant plant species in parasitism and grafting.

    Okayasu K, Aoki K, Kurotani KI, Notaguchi M

    Communicative & integrative biology   Vol. 14 ( 1 ) page: 21 - 23   2021.1

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    Publisher:Communicative and Integrative Biology  

    DOI: 10.1080/19420889.2021.1877016

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  5. An in vitro grafting method to quantify mechanical forces of adhering tissues

    Kawakatsu Yaichi, Sawai Yu, Kurotani Ken-ichi, Shiratake Katsuhiro, Notaguchi Michitaka

    PLANT BIOTECHNOLOGY   Vol. 37 ( 4 ) page: 451 - 458   2020.12

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    Publisher:Plant Biotechnology  

    <p>Grafting is an indispensable agricultural technology for propagating useful tree varieties and obtaining beneficial traits of two varieties/species—as stock and scion—at the same time. Recent studies of molecular events during grafting have revealed dynamic physiological and transcriptomic changes. Strategies focused on specific grafting steps are needed to further associate each physiological and molecular event with those steps. In this study, we developed a method to investigate the tissue adhesion event, an early grafting step, by improving an artificial in vitro grafting system in which two pieces of 1.5-mm thick <i>Nicotiana benthamiana</i> cut stem sections were combined and cultured on medium. We prepared a silicone sheet containing five special cutouts for adhesion of cut stem slices. We quantitatively measured the adhesive force at these grafting interfaces using a force gauge and found that graft adhesion started 2 days after grafting, with the adhesive force gradually increasing over time. After confirming the positive effect of auxin on grafting by this method, we tested the effect of cellulase treatment and observed significant enhancement of graft tissue adhesion. Compared with the addition of auxin or cellulase individually, the adhesive force was stronger when both auxin and cellulase were added simultaneously. The in vitro grafting method developed in this study is thus useful for examining the process of graft adhesion.</p>

    DOI: 10.5511/plantbiotechnology.20.0925a

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  6. High-quality sugar production by osgcs1 rice

    Honma Yujiro, Adhikari Prakash Babu, Kuwata Keiko, Kagenishi Tomoko, Yokawa Ken, Notaguchi Michitaka, Kurotani Kenichi, Toda Erika, Bessho-Uehara Kanako, Liu Xiaoyan, Zhu Shaowei, Wu Xiaoyan, Kasahara Ryushiro D.

    COMMUNICATIONS BIOLOGY   Vol. 3 ( 1 )   2020.10

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    Publisher:Communications Biology  

    DOI: 10.1038/s42003-020-01329-x

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  7. Ideal agriculture: Basic research and field technology supporting sustainable and innovative crop production

    Senoo Keishi, Fuji Masako, Utami Yuniar Devi, Shimizu Yukiko, Saijo Yusuke, Ohmori Yoshihiro, Fujiwara Toru, Shiratori Yutaka, Ota Sayuri, Masuda Yoko, Itoh Hideomi, Notaguchi Michitaka, Tabata Ryo, Okayasu Koji, Sawai Yu, Suzuki Takamasa, Kurotani Ken-ichi

    Japanese Journal of Soil Science and Plant Nutrition   Vol. 91 ( 2 ) page: 94 - 98   2020

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    Publisher:Japanese Society of Soil Science and Plant Nutrition  

    DOI: 10.20710/dojo.91.2_94

  8. Ⅱ-2 接木技術の再考 ストレス土壌の活用を目指して(Ⅱ 理想の農業を追求する-サステイナブルで革新的な食糧生産を支える基礎研究と現場技術,2019年静岡大会)

    野田口 理孝, 田畑 亮, 岡安 浩次, 澤井 優, 鈴木 孝征, 黒谷 賢一

    日本土壌肥料学会講演要旨集   Vol. 65 ( 0 ) page: 171 - 171   2019

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    Publisher:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.65.0_171_1

  9. Re-Evaluation of Florigen Transport Kinetics with Separation of Functions by Mutations That Uncouple Flowering Initiation and Long-Distance Transport

    Endo Motomu, Yoshida Masayasu, Sasaki Youhei, Negishi Katsuya, Horikawa Kobo, Daimon Yasufumi, Kurotani Ken-Ichi, Notaguchi Michitaka, Abe Mitsutomo, Araki Takashi

    PLANT AND CELL PHYSIOLOGY   Vol. 59 ( 8 ) page: 1621 - 1629   2018.8

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    Publisher:Plant and Cell Physiology  

    DOI: 10.1093/pcp/pcy063

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  10. Stress Tolerance Profiling of a Collection of Extant Salt-Tolerant Rice Varieties and Transgenic Plants Overexpressing Abiotic Stress Tolerance Genes

    Kurotani Ken-ichi, Yamanaka Kazumasa, Toda Yosuke, Ogawa Daisuke, Tanaka Maiko, Kozawa Hirotsugu, Nakamura Hidemitsu, Hakata Makoto, Ichikawa Hiroaki, Hattori Tsukaho, Takeda Shin

    PLANT AND CELL PHYSIOLOGY   Vol. 56 ( 10 ) page: 1867 - 1876   2015.10

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    Publisher:Plant and Cell Physiology  

    DOI: 10.1093/pcp/pcv106

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  11. Overexpression of a CYP94 family gene CYP94C2b increases internode length and plant height in rice

    Kurotani K.I.

    Plant Signaling and Behavior   Vol. 10 ( 7 )   2015.7

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    Publisher:Plant Signaling and Behavior  

    DOI: 10.1080/15592324.2015.1046667

    Scopus

  12. Elevated Levels of CYP94 Family Gene Expression Alleviate the Jasmonate Response and Enhance Salt Tolerance in Rice

    Kurotani Ken-ichi, Hayashi Kenji, Hatanaka Saki, Toda Yosuke, Ogawa Daisuke, Ichikawa Hiroaki, Ishimaru Yasuhiro, Tashita Ryo, Suzuki Takeshi, Ueda Minoru, Hattori Tsukaho, Takeda Shin

    PLANT AND CELL PHYSIOLOGY   Vol. 56 ( 4 ) page: 779 - 789   2015.4

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    Publisher:Plant and Cell Physiology  

    DOI: 10.1093/pcp/pcv006

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  13. Overexpression of the JAZ factors with mutated jas domains causes pleiotropic defects in rice spikelet development.

    Hori Y, Kurotani K, Toda Y, Hattori T, Takeda S

    Plant signaling & behavior   Vol. 9 ( 10 ) page: e970414   2014

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    Publisher:Plant Signaling and Behavior  

    DOI: 10.4161/15592316.2014.970414

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  14. RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation

    Toda Yosuke, Tanaka Maiko, Ogawa Daisuke, Kurata Kyo, Kurotani Ken-ichi, Habu Yoshiki, Ando Tsuyu, Sugimoto Kazuhiko, Mitsuda Nobutaka, Katoh Etsuko, Abe Kiyomi, Miyao Akio, Hirochika Hirohiko, Hattori Tsukaho, Takeda Shin

    PLANT CELL   Vol. 25 ( 5 ) page: 1709 - 1725   2013.5

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  15. BRANCHED1 interacts with FLOWERING LOCUS T to repress the floral transition of the axillary meristems in Arabidopsis.

    Niwa M, Daimon Y, Kurotani K, Higo A, Pruneda-Paz JL, Breton G, Mitsuda N, Kay SA, Ohme-Takagi M, Endo M, Araki T

    The Plant cell   Vol. 25 ( 4 ) page: 1228 - 42   2013.4

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    Publisher:Plant Cell  

    DOI: 10.1105/tpc.112.109090

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  16. Phytochrome B regulates Heading date 1 (Hd1)-mediated expression of rice florigen Hd3a and critical day length in rice

    Ishikawa Ryo, Aoki Mayumi, Kurotani Ken-ichi, Yokoi Shuji, Shinomura Tomoko, Takano Makoto, Shimamoto Ko

    MOLECULAR GENETICS AND GENOMICS   Vol. 285 ( 6 ) page: 461 - 470   2011.6

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    Publisher:Molecular Genetics and Genomics  

    DOI: 10.1007/s00438-011-0621-4

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  17. Expression of a bacterial flagellin gene triggers plant immune responses and confers disease resistance in transgenic rice plants.

    Takakura Y, Che FS, Ishida Y, Tsutsumi F, Kurotani K, Usami S, Isogai A, Imaseki H

    Molecular plant pathology   Vol. 9 ( 4 ) page: 525 - 9   2008.7

  18. Identification of dynamin as an interactor of rice GIGANTEA by tandem affinity purification (TAP).

    Abe M, Fujiwara M, Kurotani K, Yokoi S, Shimamoto K

    Plant & cell physiology   Vol. 49 ( 3 ) page: 420 - 32   2008.3

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  19. Effect of photooxidative destruction of chloroplasts on the expression of nuclear genes for C4 photosynthesis and for chloroplast biogenesis in maize.

    Tamada Y, Imanari E, Kurotani K, Nakai M, Andreo CS, Izui K

    Journal of plant physiology   Vol. 160 ( 1 ) page: 3 - 8   2003.1

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MISC 4

  1. Glycan-hydrolyzing enzymes link plants Invited

    Ken-ichi Kurotani / Michitaka Notaguchi

    Glycoforum   Vol. 24 ( 1 ) page: A2   2021.2

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    Authorship:Lead author   Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: 10.32285/glycoforum.24A2J

  2. 接木の成立メカニズムの解明と異科接木の農業利用 Invited

    黒谷賢一 , 野田口理孝

    バイオサイエンスとインダストリー(B&I)   Vol. 79 ( 1 )   2021.1

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    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  3. Screening of rice genes conferring tolerance to environmental stresses using rice FOX lines.

    Kurotani Ken-ichi, Hattori Tsukaho, Takeda Shin, Yamanaka Kazumasa, Ogawa Daisuke, Mizutani Megumi, Toda Yousuke, Tanaka Maiko, Yamamoto Akiko, Kato Hirokazu, Ichikawa Hiroaki

    Plant and Cell Physiology Supplement   Vol. 2010 ( 0 ) page: 922 - 922   2010

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    Publisher:The Japanese Society of Plant Physiologists  

    To identify rice genes that confer tolerance to environmental stresses, we are taking advantage of FOX Hunting System. In terms of physiology, abiotic stresses such as drought, salinity, heat or cold stress, provoke overlapping responses in plants, indicating the mechanisms of tolerance to these environmental stresses may share the same machinery. In this study, we are planning to perform two types of screening. The first one is "comprehensive FOX hunting" (c-FOX), where transgenic rice lines containing rice full-length cDNA are screened at T0 and/or T1 generations under salinity stress condition. We attempt to screen 2232 T1 transgenic lines, kindly provided by NIAS, and 215 independent T0 lines. The second strategy is "targeted FOX hunting" (t-FOX), for which 100 candidate genes (TOP100) are pre-selected based on the previous studies using multiple plant species, and used for examination resources to test whether overexpression of those genes confers stress tolerance under various environmental conditions. We are also evaluating existing rice cultivars with higher stress tolerance, to test as a host plant for further combinational analysis of the selected FOX genes.

    DOI: 10.14841/jspp.2010.0.0922.0

  4. Screening of rice genes conferring tolerance to environmental stresses by Rice FOX Hunting System

    Kurotani Ken-ichi, Hattori Tsukaho, Takeda Shin, Yamanaka Kazumasa, Ogawa Daisuke, Mizutani Megumi, Toda Yousuke, Tanaka Maiko, Yamamoto Akiko, Katou Hirokazu, Ichikawa Hiroaki

    Plant and Cell Physiology Supplement   Vol. 2009 ( 0 ) page: 923 - 923   2009

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    Publisher:The Japanese Society of Plant Physiologists  

    To identify rice genes that confer tolerance to environmental stresses, we are taking advantage of FOX Hunting System. In this study, we are planning to perform two types of screening. The first one is "conventional FOX hunting" (c-FOX), where transgenic rice lines containing rice full-length cDNA (rice FOX lines) are screened at T0 and T1 generations under salinity stress condition. The second strategy is "targeted FOX hunting" (t-FOX), for which ~100 candidate genes (TOP100) are listed for examination under various stress conditions to select most useful genes conferring stress tolerance. We are also evaluating rice cultivars with higher stress tolerance, to test as a host plant for further combinational analysis of the selected FOX genes. Details of the project and how to establish extremely tolerant lines using the selected materials will be discussed.

    DOI: 10.14841/jspp.2009.0.0923.0

KAKENHI (Grants-in-Aid for Scientific Research) 2

  1. 植物の移行性mRNAの輸送機構および機能に関する研究

    Grant number:20H03273  2020.4 - 2023.3

    野田口 理孝

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    Authorship:Coinvestigator(s) 

    多細胞生物は、個々に機能分化した異なる器官、異なる組織が協調して働くことで、個体レベルの機能をより適切に発揮させていると考えられる。植物の全身性シグナル伝達は、篩管や道管を介した植物ホルモン、small RNA、mRNA、ペプチド、タンパク質等の分子送達で果たされていることが知られ、花成や栄養飢餓応答、乾燥ストレス応答等が全身的に制御されていることが明らかとなってきた。本研究では、それらの全身移行性分子のなかでも、生物学的な意義が未だに不明であるmRNAについて、その輸送の分子機構と植物の発生成長における働きを明らかにする。

  2. DNA損傷応答蛋白質の網羅的インタラクトーム解析

    Grant number:18K11639  2018.4 - 2021.3

    黒谷 賢一

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    Authorship:Principal investigator 

    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

    DNA損傷応答 (DNA damage response, DDR)はゲノムの安定化に極めて重要な反応である。DDR蛋白質の細胞内での機能を理解するためには、蛋白質-蛋白質間の相互作用 (Protein-protein interaction, PPI)ネットワークを詳細に知る必要がある。本研究では、精密質量分析装置を利用した、ヒト細胞におけるDDR蛋白質の網羅的インタラクトーム解析を実施する。新規のDDR蛋白質の同定ならびにその機能解析を実施することで、DDRの理解を深めることを目的とする。
    2019年度は、インタラクトーム解析を生理的条件下で実施するために、ゲノム編集技術を利用し、標的遺伝子のC末端領域にタグ遺伝子配列と薬剤選択遺伝子配列の導入を行った。その結果、ゲノム編集後に細胞内で比較的高コピー数の蛋白質 (蛍光顕微鏡にてmCloverのシグナルで判断)を発現する遺伝子を複数見出した。 ゲノム編集により、mClover配列とともにStrep配列を挿入しており、Strep-Tactinシステムを用いて、標的蛋白質を濃縮するための条件検討を行った。しかしながら、精密質量分析装置を用いた網羅的インタラクトーム解析に十分な標的蛋白質の量を回収することができなかった。解析に必要な十分な標的蛋白質量を確保するためには、解析細胞数の増量、もしくはStrep-Tactinシステムによるプルダウン効率の上昇が必要であると考えられる。
    網羅的インタラクトーム解析を内在性蛋白質に対して実施するために、ゲノム編集技術を利用した、mClover、Strep、薬剤耐性遺伝子の導入を実施した。その結果、複数の標的遺伝子に対するゲノム編集をDNA/蛋白質レベルで確認することができた。標的遺伝子に対するゲノム編集が確認された細胞に対して、Strep-Tactinシステムを用いたプルダウン法を実施したが、精密質量分析装置を用いた網羅的インタラクトーム解析に十分な標的蛋白質量を確保することができなかった。標的遺伝子の細胞内での発現量が低い場合においても、網羅的インタラクトーム解析に必要十分な蛋白質量を確保するための条件検討を実施する必要性が出たため。
    2020年度は、ゲノム編集が確認された細胞から蛋白質を抽出し、精密質量分析装置を用いた網羅的インタラクトーム解析に十分な標的蛋白質量を確保するための条件検討を実施する。Strep-Tactinシステムを用いたプルダウン法の効率を上げるため、現状のStrep配列1個からタンデムに2個並べたコンストラクトを作製する。また、解析細胞数についても条件検討を行う。外来遺伝子を細胞へ導入し発現させた蛋白質に対しても、インタラクトーム解析の実施準備を行う。網羅的インタラクトーム解析に必要な蛋白質を安定して回収することが可能となった後に、精密質量分析のためのサンプル調整について条件検討を実施する。DDR蛋白質のインタラクトーム解析により、新規蛋白質-蛋白質間相互作用、ならびに機能未知の蛋白質との相互作用が検出された際には、分子生物学的手法を用いた解析を実施する。具体的には、標的蛋白質に対する抗体を用いた免疫沈降法により、質量分析装置で得られた実験結果の確認を行う。また、機能未知蛋白質の細胞内で役割を検討するため、siRNA法ならびにゲノム編集技術により機能喪失させた細胞を作製し、DDRへの関与の有無を検討する。

Industrial property rights 3

  1. 環境ストレス耐性を付与する遺伝子及びその利用

    武田真 , 服部束穂 , 黒谷賢一 , 市川裕章

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    Application no:特願2015-142503  Date applied:2015.7

    Announcement no:特開2016-103994  Date announced:2016.6

  2. フラジェリンタンパク質による病害抵抗性植物を作出する方法

    高倉由光、堤史樹、黒谷賢一、石田祐二、蔡晃植

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    Application no:特願2002-187575 

    Announcement no:特開2004-024160 

  3. 植物の開花を遅延させるDNA発現カセット

    島本功, 黒谷賢一

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    Application no:特願2006-232039 

    Announcement no:特開2008-54512