2021/06/21 更新

写真a

ババ ヨシノブ
馬場 嘉信
BABA, Yoshinobu
所属
名古屋大学 未来社会創造機構 ナノライフシステム研究所 量子科学技術・量子生命科学研究部門 教授
職名
教授

学位 1

  1. 理学博士 ( 1986年3月   九州大学 ) 

研究キーワード 5

  1. 分析化学

  2. 個別化医療

  3. ゲノム

  4. バイオテクノロジー

  5. ナノテクノロジー

研究分野 4

  1. ナノテク・材料 / ナノマイクロシステム  / マイクロ・ナノデバイス

  2. ナノテク・材料 / ナノバイオサイエンス  / ナノ材料・ナノバイオサイエンス

  3. ライフサイエンス / ゲノム生物学  / 応用ゲノム科学

  4. ナノテク・材料 / 分析化学  / 分析化学

現在の研究課題とSDGs 6

  1. いきいき百歳社会実現

  2. 次世代ナノバイオデバイスの創成とゲノム医療への応用に関する研究

  3. ナノバイオデバイス、AI、量子生命科学による次世代ヘルスケア開拓

  4. ナノ空間における生体分子・細胞の構造・機能解析に関する研究

  5. システムバイオロジーを目指した計測技術開発に関する研究

  6. 1分子DNA解析と1分子DNAマニピュレーションに関する研究

▼全件表示

経歴 42

  1. 量子科学技術研究開発機構   量子生命・医学部門 量子生命科学研究所   所長(センター長)

    2021年4月 - 現在

  2. 量子科学技術研究開発機構   量子生命科学研究拠点センター   所長(センター長)

    2021年2月 - 現在

  3. ONCOLille, フランスLille がん学際領域研究所   国際科学諮問委員会   特別招へい教授

    2020年12月 - 現在

  4. 名古屋大学   未来社会創造機構 ナノライフシステム研究所 量子科学技術・量子生命科学研究部門   教授

    2020年7月 - 現在

  5. 新エネルギー・産業技術総合開発機構(NEDO)   技術戦略研究センター(TSC) フェロー   フェロー

    2020年4月 - 現在

  6. 量子科学技術研究開発機構   量子生命科学領域   所長(センター長)

    2019年4月 - 2021年3月

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    国名:日本国

  7. 名古屋大学   高等研究院 運営推進委員   教授

    2019年1月 - 現在

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    国名:日本国

  8. 名古屋大学   未来社会創造機構 ナノライフシステム研究所   所長(センター長)

    2018年10月 - 現在

  9. JST   CREST 「細胞外微粒子に起因する生命現象の解明とその制御に向けた基盤技術の創出」   所長(センター長)

    2017年4月 - 現在

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    国名:日本国

  10. 東京大学   大学院理学系研究科 専攻共通流通講座(化学)   教授

    2017年4月 - 2019年3月

      詳細を見る

    国名:日本国

  11. 高雄医科大学   薬学部   特別招へい教授

    2017年2月 - 現在

      詳細を見る

    国名:日本国

  12. 名古屋大学   先端ナノバイオデバイス研究センター   所長(センター長)

    2015年4月 - 2019年3月

      詳細を見る

    国名:日本国

  13. 名古屋大学   ■廃止組織■ 先端ナノバイオデバイス研究センター   所長(センター長)

    2015年4月 - 2019年3月

  14. 名古屋大学 未来社会創造機構 教授

    2014年4月 - 現在

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    国名:日本国

  15. 名古屋大学   未来社会創造機構   教授

    2014年4月 - 現在

      詳細を見る

    国名:日本国

  16. 名古屋大学   未来社会創造機構   教授

    2014年4月 - 現在

  17. 名古屋大学シンクロトロン光研究センター センター長

    2012年4月 - 2015年3月

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    国名:日本国

  18. 名古屋大学   シンクロトロン光研究センター   所長(センター長)

    2012年4月 - 2015年3月

  19. 名古屋大学シンクロトロン光研究センター 副センター長

    2011年8月 - 2012年3月

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    国名:日本国

  20. 名古屋大学   シンクロトロン光研究センター   副センター長

    2011年8月 - 2012年3月

  21. 名古屋大学   革新ナノバイオデバイス研究センター   所長(センター長)

    2010年12月 - 2015年3月

  22. 名古屋大学 総長補佐(産学官連携担当・研究推進担当)

    2010年4月 - 2012年3月

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    国名:日本国

  23. 産業技術総合研究所 健康工学研究部門 研究顧問 併任

    2010年4月

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    国名:日本国

  24. 名古屋大学革新ナノバイオデバイス研究センター センター長(併任)

    2010年3月 - 2015年3月

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    国名:日本国

  25. 名古屋大学   大学院医学系研究科   協力講座教授

    2008年4月 - 現在

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    国名:日本国

  26. 名古屋大学   総長補佐(産学官連携担当・研究推進担当)

    2008年4月 - 2012年3月

  27. 名古屋大学 総長補佐(研究推進担当)

    2008年4月 - 2010年3月

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    国名:日本国

  28. 自然科学研究機構・分子科学研究所・客員教授

    2007年4月 - 2009年3月

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    国名:日本国

  29. 名古屋大学   プラズマナノ工学研究センター 教授

    2006年10月 - 現在

  30. 名古屋大学大学院工学研究科プラズマナノ工学研究センター 教授(併任)

    2006年10月

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    国名:日本国

  31. 名古屋大学   予防早期医療創成センター   教授

    2006年8月 - 現在

  32. 名古屋大学予防早期医療創成センター 教授 (併任)

    2006年8月

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    国名:日本国

  33. 産業技術総合研究所 健康工学研究センター 副センター長 併任

    2005年4月 - 2010年3月

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    国名:日本国

  34. 名古屋大学大学院工学研究科 教授

    2004年10月

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    国名:日本国

  35. 産業技術総合研究所 単一分子生体ナノ計測研究ラボ ラボ長 併任

    2002年10月 - 2005年3月

      詳細を見る

    国名:日本国

  36. 徳島大学薬学部 教授

    1997年4月 - 2005年3月

      詳細を見る

    国名:日本国

  37. 神戸薬科大学薬学部 助教授

    1996年4月 - 1997年3月

      詳細を見る

    国名:日本国

  38. 神戸女子薬科大学薬学部 講師

    1990年4月 - 1996年3月

      詳細を見る

    国名:日本国

  39. 大分大学教育学部 講師

    1988年4月 - 1990年3月

      詳細を見る

    国名:日本国

  40. 大分大学教育学部 助手

    1986年6月 - 1988年3月

      詳細を見る

    国名:日本国

  41. 日本学術振興会 特別研究員

    1986年4月 - 1986年5月

      詳細を見る

    国名:日本国

  42. 量子科学技術研究開発機構   量子生命科学研究拠点センター   所長(センター長)

▼全件表示

学歴 3

  1. 九州大学   理学研究科   化学専攻

    1983年4月 - 1986年3月

      詳細を見る

    国名: 日本国

  2. 九州大学   理学研究科   化学専攻

    1981年4月 - 1983年3月

      詳細を見る

    国名: 日本国

  3. 九州大学   理学部   化学科

    1977年4月 - 1981年3月

      詳細を見る

    国名: 日本国

所属学協会 20

  1. 日本化学会   生命化学研究会会長

    2002年4月 - 2005年3月

  2. 化学とマイクロ・ナノシステム研究会   会長

    2006年4月 - 2009年3月

  3. アメリカ化学会   Anal. Chem.副編集長

  4. 王立化学協会   フェロー, Lab on Chip編集委員、Nanoscale編集委員

    2005年4月 - 現在

  5. アメリカ科学振興協会

  6. アメリカ応用物理学会   Biomicrofluidics編集委員

  7. 国際ナノメディシンアカデミー   理事

  8. アジアパシフィックナノメディシンアカデミー   会長

  9. 電気学会   バイオ極限分析調査専門委員会委員長

    2006年4月 - 2009年3月

  10. アメリカナノメディシンアカデミー   フェロー

    2006年4月 - 2007年3月

  11. 日本化学会   生命化学研究会幹事

    2004年4月 - 現在

  12. 日本薬学会   薬学研究ビジョン部会会長

    2004年4月 - 2007年3月

  13. 日本分析化学会

    1984年4月 - 現在

  14. 日本電気泳動学会

  15. 日本バイオマテリアル学会   評議員

  16. 近畿化学協会

  17. 日本希土類学会

  18. 化学センサ研究会

  19. クロマトグラフィー科学会

  20. 日本分子生物学会

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受賞 17

  1. 日本化学会 学会賞

    2021年3月   日本化学会   ナノバイオデバイスによるバイオ計測化学・バイオ医工学の革新

     詳細を見る

    受賞区分:国内外の国際的学術賞  受賞国:日本国

  2. 化学とマイクロ・ナノシステム学会 学会賞

    2021年3月   化学とマイクロ・ナノシステム学会  

  3. 寺部茂賞

    2016年11月   日本分析化学会   ナノバイオデバイスの創製と生体分析への展開

    馬場嘉信

     詳細を見る

    受賞区分:国内学会・会議・シンポジウム等の賞  受賞国:日本国

  4. 文部科学大臣表彰 科学技術賞 研究部門

    2016年4月   文部科学省  

     詳細を見る

    受賞国:日本国

  5. 日本分析化学会 学会賞

    2015年9月   日本分析化学会  

     詳細を見る

    受賞国:日本国

  6. クロマトグラフィー科学会 学会賞

    2014年12月   クロマトグラフィー科学会  

     詳細を見る

    受賞国:日本国

  7. モノづくり連携大賞特別賞

    2009年11月   内閣府  

     詳細を見る

    受賞国:日本国

  8. 日本化学会学術賞

    2008年3月   日本化学会  

     詳細を見る

    受賞国:日本国

  9. フェロー アメリカナノメディシンアカデミー

    2006年  

  10. 第28回応用物理学会論文賞

    2006年   社団法人 応用物理学会  

     詳細を見る

    受賞国:日本国

  11. フェロー 英国王立化学会

    2005年   英国王立化学会  

  12. 2004年度メルク賞

    2004年   メルク賞選考委員会  

  13. 平成16年度日本トキシコロジー学会賞(田邊賞)

    2004年  

     詳細を見る

    受賞国:日本国

  14. 平成15年度竹田国際貢

    2003年  

     詳細を見る

    受賞国:日本国

  15. 第38回徳島新聞賞科学賞

    2002年  

     詳細を見る

    受賞国:日本国

  16. 平成9年度日本薬学会奨励賞

    1997年  

     詳細を見る

    受賞国:日本国

  17. 平成3年度日本薬学会近畿支部奨励賞

    1992年  

     詳細を見る

    受賞国:日本国

▼全件表示

 

論文 1169

  1. Clinical and Prognostic Values of ALBI Score in Patients With Acute Heart Failure

    Matsue Y., Kagiyama N., Yamaguchi T., Kuroda S., Okumura T., Kida K., Mizuno A., Oishi S., Inuzuka Y., Akiyama E., Matsukawa R., Kato K., Suzuki S., Naruke T., Yoshioka K., Miyoshi T., Baba Y., Yamamoto M., Mizutani K., Yoshida K., Kitai T.

    Heart Lung and Circulation   29 巻 ( 9 ) 頁: 1328 - 1337   2020年9月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Heart Lung and Circulation  

    Background: Although liver dysfunction is one of the common complications in patients with acute heart failure (AHF), no integrated marker has been defined. The albumin-bilirubin (ALBI) score has recently been proposed as a novel, clinically-applicable scoring system for liver dysfunction. We investigated the utility of the ALBI score in patients with AHF compared to that for a preexisting liver dysfunction score, the Model of End-Stage Liver Disease Excluding prothrombin time (MELD XI) score. Methods: We evaluated ALBI and MELD XI scores in 1,190 AHF patients enrolled in the prospective, multicentre Registry Focused on Very Early Presentation and Treatment in Emergency Department of Acute Heart Failure study. The associations between the two scores and the clinical profile and prognostic predictive ability for 1-year mortality were evaluated. Results: The mean MELD XI and ALBI scores were 13.4±4.8 and -2.25±0.48, respectively. A higher ALBI score, but not higher MELD XI score, was associated with findings of fluid overload. After adjusting for pre-existing prognostic factors, the ALBI score (HR 2.11, 95% CI: 1.60–2.79, p<0.001), but not the MELD XI score (HR 1.02, 95% CI: 0.99–1.06, p=0.242), was associated with 1-year mortality. Likewise, area under the receiver-operator-characteristic curves for 1-year mortality significantly increased when the ALBI score (0.71 vs. 0.74, p=0.020), but not the MELD XI score (0.71 vs. 0.72, p=0.448), was added to the pre-existing risk factors. Conclusions: The ALBI score is potentially a suitable liver dysfunction marker that incorporates information on fluid overload and prognosis in patients with AHF. These results provide new insights into heart-liver interactions in AHF patients.

    DOI: 10.1016/j.hlc.2019.12.003

    Scopus

  2. Analysis and survey of pm<inf>2.5</inf> from a biological viewpoint at kyushu university ito campus

    Miura K., Ide S., Naito T., Shimada T., Yasui T., Baba Y., Kaji N.

    Bunseki Kagaku   69 巻 ( 12 ) 頁: 741 - 746   2020年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Bunseki Kagaku  

    Fine particulate matter sized 2.5 μm or less, referred to as PM2.5, causes serious air pollution in urban cities and is responsible for several health problems, such as respiratory disease and asthma in urban populations. Although seasonal Asian dust, called Kosa (~ 5 μm), has been extensively studied for over decades, and has been shown to be an allergen in Japan, the influence of PM2.5 on human health has remained unclear due to the intrinsic chemical and biological complexity of isolating variables for study. In this study, PM2.5 was collected at Ito Campus in Kyushu University, Fukuoka, located in extreme western Japan and its physical and biological characteristics were analyzed. The presence of bacteria, Firmicutes genus Bacillus, Lysinibacillus, Paenibacillus, Gracilibacillus, and Solibacillus in the collected PM2.5 sample was confirmed by culturing and conducting DNA sequencing of their 16S rRNA gene.

    DOI: 10.2116/bunsekikagaku.69.741

    Scopus

    CiNii Article

  3. Cell deposition and isolation with micropipette control over liquid interface motion in microfluidic channel

    Onoshima D., Yukawa H., Hattori Y., Ishikawa K., Hori M., Baba Y.

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017     頁: 679 - 680   2020年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017  

    We developed a microfluidic chip for depositing single cells in microwells using simple micropipette operation. Cells were delivered to microwells by the meniscus motion of liquid interface. The residue deposits of cells were redistributed with air injection, and the isolated single cells were stored in microwells. Different microwell sizes and depths were studied to evaluate the trapping possibility of cells. Medium replacement and cell viability staining with the isolated single cells were achieved in microwells.

    Scopus

  4. A zinc oxide nanowires device for extracellular vesicles isolation and purification

    Thiodorus I.A., Kaji N., Yasui T., Baba Y.

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017     頁: 886 - 887   2020年

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017  

    In this study, we have developed extracellular vesicles (EVs) isolation and purification using a ZnO nanowires microfluidic device with the principle of ion exchange chromatography. We had successfully purify EVs in large concentration without any chemical or physical damage on EVs membrane.

    Scopus

  5. Extracellular vesicels separation by electroosmotic flow-driven deterministic lateral displacement in nanopillar chips

    Kaji N., Hattori Y., Yasaki H., Yasui T., Baba Y.

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017     頁: 904 - 905   2020年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017  

    Here we first demonstrate extracellular vesicles (EVs) separation by electroosmotic flow (EOF)-driven deterministic lateral displacement (DLD) in a nanopillar array. The proposed EOF-based separation technique solved the issue associated with high input pressure more than a few hundred kPa to drive the solution in the nanopillar array and offered an easy-to-use and high-throughput DLD separation technique for nanometer-scale objects.

    Scopus

  6. PM2.5 collection into aqueous solution via hydrophilic oxide nanowire surface

    Shimada T., Yasui T., Hibara A., Yasaki H., Yanagida T., Hara M., Kaji N., Kanai M., Nagashima K., Kawai T., Baba Y.

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017     頁: 1433 - 1434   2020年

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017  

    For collection of particulate matters with the size less than or equal to 2.5 μm (PM2.5) into aqueous solutions, we propose a methodology for realizing the PM2.5 collection into aqueous solution via hydrophilic surface of oxide nanowires. The developed surface allowed to form a water film over 300 days, and to collect PM2.5 into a water film from air and recover the solution for followed-by PM2.5 analysis, continuously. This device would be a new platform to collect air-floating PM2.5 into aqueous solution for the subsequent detection of PM2.5 by using online micropore detector [1,2].

    Scopus

  7. Nanowires enabling cancer diagnosis from 1 mL urine

    Naganawa T., Yasui T., Yanagida T., Kaji N., Kanai M., Nagashima K., Kawai T., Baba Y.

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017     頁: 1271 - 1272   2020年

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017  

    We have far developed the device with ZnO nanowires for in-situ miRNA collection from 1 mL urine. Here, we collected 48 miRNA samples from 24 healthy subjects and 24 cancer subjects. And then, we conducted two kinds of analisis: heatmap and AUC (Area Under the Curve) analysis. After the analysis, we could show the results that miRNA types are different between before and after cancer formation, and that some biomarker candidates were identified. This methodology will be expected to be a pioneer for the new cancer diagnosis from non-invasive miRNA analysis.

    Scopus

  8. Nanobiodevices and AI for society 5.0: Super smart society

    Baba Y.

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017     頁: 1 - 2   2020年

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017  

    Nanobiodevice is a piece of contrivance, equipment, machine, or component, which is created by the overlapping multidisciplinary activities associated with nanotechnology and biotechnology. We have developed nanobiodevices for society 5.0; super smart society, including immuno-wall microfluidic devices for healthcare and point-of-care testing, nanopillar-nanopore nanofluidic devices for single DNA and microRNA sequencing, nanowire nanofluidic devices for exosome analysis, AI-powerd IoNT nanosensors, and quantum switching intra vital imaging of iPS cells.

    Scopus

  9. Millisecond mechanophenotyping of stem cells by electrical detection in microfluidic constrictions

    Kaji N., Ito S., Yasaki H., Yasui T., Yukawa H., Baba Y.

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017     頁: 832 - 833   2020年

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017  

    A microfluidic device based on electrical detection for high-throughput and practical mechanophenotyping was developed to investigate the deformability and sizes of cells in a single run. Deformability changes associated with passage of adipose tissue-derived stem cells (ASCs) were successfully detected by the device without any chemical or biological modification.

    Scopus

  10. Leukocyte depletion and size-based enrichment of circulating tumor cells with pressure-sensing microfiltration system

    Kuboyama D., Onoshima D., Kihara N., Tanaka H., Hase T., Yukawa H., Ishikawa K., Odaka H., Hasegawa Y., Hori M., Baba Y.

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017     頁: 882 - 883   2020年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017  

    We present a study of blood microfiltration system towards optimal approach of non-invasive liquid biopsy for cancer detection by circulating tumor cells (CTCs). Filtration behavior of a microfilter was experimentally analyzed for tumor cell isolation from leukocytes in whole blood. This approach achieved an average of >96% recovery of spiked tumor cells and >99% total leukocytes depletion.

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  11. Exosomal Analysis of ALK Rearrangements by Spin Column with Porous Glass Filter 国際誌

    Hatta T., Hase T., Ozawa N., Yogo N., Yukawa H., Tanaka H., Onoshima D., Sato M., Hori M., Baba Y., Hasegawa Y.

    JOURNAL OF THORACIC ONCOLOGY   14 巻 ( 10 ) 頁: S676 - S676   2019年10月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  12. Fluorescence signal amplification for sensitive enzyme immunoassay utilizing an immuno-wall 国際誌

    Nishiyama K., Kasama T., Maeki M., Ishida A., Tani H., Baba Y., Tokeshi M.

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019     頁: 689 - 690   2019年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019  

    This paper reports a novel method of signal amplification based on the accumulation of enzymatic fluorescent products at a wall-like structure (immuno-wall) in a microchannel for sensitive immunoassay. We found the function of an immuno-wall as a media for the enrichment of fluorescence molecules. We combined this function with the amplification of the fluorescence signal by an enzymatic reaction to produce a synergistic effect. The performance of the device was evaluated with human C-reactive protein (CRP) as a model target. The limit of detection of CRP was 2.5 pg/mL. This value was approximately 3 orders of magnitude lower than that obtained with a fluorescent dye-labeled antibody (1.7 ng/mL).

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  13. Effectiveness of Kangaroo Care for a Patient with Postpartum Depression and Comorbid Mother-Infant Bonding Disorder

    Takubo Y., Nemoto T., Obata Y., Baba Y., Yamaguchi T., Katagiri N., Tsujino N., Kitamura T., Mizuno M.

    Case Reports in Psychiatry   2019 巻   2019年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Case Reports in Psychiatry  

    The presently reported patient was a 37-year-old married primipara with peripartum depression comorbid with bonding disorder. Care anxiety and a lack of affection towards her baby first appeared at around the time of delivery, and the patient developed major depression at one month after the birth of her healthy baby. At first, standard treatment for major depression including the use of antidepressants, electroconvulsive therapy, and supportive psychotherapy were provided. However, bonding problems appeared to impede and obstruct the amelioration of depression. Although treatment methods for bonding disorder have not yet been established, Kangaroo Care was introduced to facilitate skin-to-skin contact. We also educated her in better parenting behavior and provided repeated motivational interviews with her family because a lack of partner and social support and personal temperament (low self-directedness and cooperativeness) were thought to be related to her bonding disorder. This case suggests the effectiveness of Kangaroo Care, which promotes a humanizing maturation of both baby and parent alike, for mothers with postpartum depression and comorbid severe bonding disorder.

    DOI: 10.1155/2019/9157214

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  14. Non-destructive dispersion of quantum dots into buffer gases toward their optical manipulation

    Kumakura M., Baba Y., Shimomura T., Takiyama T., Kameyama T., Torimoto T., Moriyasu T.

    Proceedings of SPIE - The International Society for Optical Engineering   11141 巻   2019年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Proceedings of SPIE - The International Society for Optical Engineering  

    To carry out optical manipulation and spectral measurement of isolated quantum dots (QDs) in buffer gases, we are constructing experimental setups for dispersing QDs by using droplets of their organic solutions. The droplet was generated with a mesh-type nebulizer with a piezoelectric oscillator and was monitored by observing the scattered light. The QD density was also monitored by observing the fluorescence from the QDs. In this paper, we present our recent experimental results on the dispersion of ZnS-AgInS2 solid solution QDs and AgInS2/ZnS nano-pyramid QDs into a dry nitrogen buffer gas, and discuss their isolation and optical lifetimes in comparison with our previous results on CdSe/ZnS QDs. We also discuss the experimental results of miniaturizing micro-droplets into nano-clusters.

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  15. A single bacterium and mammalian cell analysis by ionic current measurements in a microchannel

    Kaji N., Sano M., Ito S., Yasaki H., Yasui T., Yukawa H., Baba Y.

    Technical Digest - International Electron Devices Meeting, IEDM     頁: 10.3.1 - 10.3.4   2018年1月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Technical Digest - International Electron Devices Meeting, IEDM  

    A microfluidic device based on ionic current detection system for high-throughput and practical single bacteria and mammalian cell sizing was developed, and furthermore, discrimination of bacterial species and mammalian cell deformability was achieved. The highly precise sizing system based on blocking ionic current at narrow microchannel provided the information on antibiotic resistant strains of bacteria. Deformability changes associated with passage of adipose tissue-derived stem cells (ASCs) were also successfully detected by the device without any chemical or biological modification.

    DOI: 10.1109/IEDM.2017.8268363

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  16. Comparison of conservative treatment versus transcatheter arterial embolisation for the treatment of spontaneously ruptured hepatocellular carcinoma

    Shinmura K., Choi Y.H., Shimohira M., Baba Y., Ikeda S., Hayashi S., Shibamoto Y., Koriyama C., Yoshiura T.

    Polish Journal of Radiology   83 巻   頁: e311 - e318   2018年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Polish Journal of Radiology  

    Purpose: To elucidate the prognostic factors in the spontaneous rupture of hepatocellular carcinoma (HCC) and to determine whether transcatheter arterial embolisation (TAE) is associated with better prognosis compared to conservative treatment. Material and methods: A retrospective multicentre study was conducted involving 71 patients with spontaneous rupture of HCC. A conservative treatment group (Cons T group) included 20 patients, while a transcatheter arterial embolisation group (TAE group) included 51 patients. Results: The median survival time (MST) in the Cons T group was only 16 days and the survival rate was 39% at one month, whereas the MST in the TAE group was 28 days and the one month survival rate was 63%. However, there is no statistically significant difference in the overall survival between Cons T and TAE groups (p = 0.213). Multivariable analysis identified only the presence of distant metastasis as an independent prognostic factor (p = 0.023). A subanalysis including patients without distant metastasis showed that the presence of portal vein tumour thrombosis was a significant prognostic factor (p = 0.015). Conclusions: Distant metastasis appears to be a prognostic factor in spontaneous rupture of HCC. In cases without distant metastasis, portal vein tumour thrombosis could influence the prognosis. Our data failed to prove any benefit of TAE as the primary management.

    DOI: 10.5114/pjr.2018.77024

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  17. Microfluidic immunoassay devices as next-generation cancer and medical diagnostics platform

    Kasama T., Baba Y., Tokeshi M.

    Next Generation Point-of-care Biomedical Sensors Technologies for Cancer Diagnosis     頁: 305 - 322   2017年12月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Next Generation Point-of-care Biomedical Sensors Technologies for Cancer Diagnosis  

    DOI: 10.1007/978-981-10-4726-8_13

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  18. Design of wave-shaped-micro-channel for active transportation of C. elegans

    Baba Y., Nakajima M., Ichikawa A., Fukuda T.

    2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016     2017年1月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016  

    A wave-micro-channel was presented to realize active transportation of C. elegans into a micro-channel. Moving velocities of C. elegans were measured in different designs. C. elegans was able to enter the wave type microchannel, and not able to enter the straight type micro channel. The moving velocity was on 160μm/s in case1, and 297 μm/s in case2 experimentally. The proposed device is designed to observe the behavior of C. elegans based on chemotaxis in an aqueous environment which is easily replaceable of the chemical substance inside micro-channels.

    DOI: 10.1109/MHS.2016.7824209

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  19. Anhedonia and its correlation with clinical aspects in Parkinson's disease

    Nagayama H., Maeda T., Uchiyama T., Hashimoto M., Nomoto N., Kano O., Takahashi T., Terashi H., Hamada S., Hasegawa T., Hatano T., Takahashi T., Baba Y., Sengoku R., Watanabe H., Inoue M., Kadowaki T., Kaneko S., Shimura H., Kubo S.i.

    Journal of the Neurological Sciences   372 巻   頁: 403 - 407   2017年1月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Journal of the Neurological Sciences  

    Anhedonia is one of the non-motor symptoms observed in the Parkinson's disease (PD). However, there is no clear relationship between anhedonia and its correlation with other symptoms of PD. The aim of this study is to evaluate the characteristics of anhedonia and its correlation with clinical aspects of PD in a relatively large cohort. We enrolled 318 patients with PD and 62 control subjects for this study. Patients and subjects were tested using the Snaith–Hamilton Pleasure Scale Japanese version and the Beck Depression Inventory 2nd edition for the assessment of anhedonia and depression. We also investigated the correlation among clinical aspects of PD, anhedonia, and depression in patients with PD. The Snaith–Hamilton Pleasure Scale Japanese version and the Beck Depression Inventory 2nd edition scores were significantly higher in patients with PD than in control subjects (p = 0.03 and p = 0.0006, respectively). All PD patients with anhedonia had a significantly higher score on the unified Parkinson's disease rating scale (UPDRS) parts I and II compared to PD patients without anhedonia. Additionally, all PD patients with depression scored significantly higher on UPDRS part I–IV than PD patients without depression. The patients with anhedonia and without depression had mild motor severity and their treatment was relatively low dosage. These results suggest that anhedonia and depression are slightly linked, but not the same. PD patients with only anhedonia may be closely linked apathy found in untreated early stages of PD.

    DOI: 10.1016/j.jns.2016.10.051

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  20. Nanopillars, Nanowires and Nanoballs for DNA and Protein Analysis

    Kaji N., Yasui T., Tokeshi M., Baba Y.

    RSC Nanoscience and Nanotechnology   2017-January 巻 ( 41 ) 頁: 76 - 98   2017年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:RSC Nanoscience and Nanotechnology  

    Recent development of nanofluidic devices using nanopillars, nanowires, and nanoballs for high-performance biomolecules analysis are reviewed in this chapter. Two approaches, "top-down" fabrication techniques and "bottom-up" self-assemble techniques, were applied to construct nanospace inside microchannels, and various biomolecules including DNA, RNA and proteins were successfully separated within a few seconds. These separation techniques enabled high throughput analysis that had never achieved by natural or synthetic polymers and explored a new bioanalytical field based on molecular dynamics in nanospace. Hybrid use of the both approaches might be promising for future home diagnostic devices and clinical applications.

    DOI: 10.1039/9781849735230-00076

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  21. The activated conformation of integrin β7 is a novel multiple myeloma-specific target for CAR T cell therapy

    Hosen N., Matsunaga Y., Hasegawa K., Matsuno H., Nakamura Y., Makita M., Watanabe K., Yoshida M., Satoh K., Morimoto S., Fujiki F., Nakajima H., Nakata J., Nishida S., Tsuboi A., Oka Y., Manabe M., Ichihara H., Aoyama Y., Mugitani A., Nakao T., Hino M., Uchibori R., Ozawa K., Baba Y., Terakura S., Wada N., Morii E., Nishimura J., Takeda K., Oji Y., Sugiyama H., Takagi J., Kumanogoh A.

    Nature Medicine   23 巻 ( 12 ) 頁: 1436 - 1443   2017年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nature Medicine  

    Cancer-specific cell-surface antigens are ideal targets for monoclonal antibody (mAb)-based immunotherapy but are likely to have previously been identified in transcriptome or proteome analyses. Here, we show that the active conformer of an integrin can serve as a specific therapeutic target for multiple myeloma (MM). We screened >10,000 anti-MM mAb clones and identified MMG49 as an MM-specific mAb specifically recognizing a subset of integrin β77 molecules. The MMG49 epitope, in the N-terminal region of the β77 chain, is predicted to be inaccessible in the resting integrin conformer but exposed in the active conformation. Elevated expression and constitutive activation of integrin β77 conferred high MMG49 reactivity on MM cells, whereas MMG49 binding was scarcely detectable in other cell types including normal integrin β77 + lymphocytes. T cells transduced with MMG49-derived chimeric antigen receptor (CAR) exerted anti-MM effects without damaging normal hematopoietic cells. Thus, MMG49 CAR T cell therapy is promising for MM, and a receptor protein with a rare but physiologically relevant conformation can serve as a cancer immunotherapy target.

    DOI: 10.1038/nm.4431

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  22. Microfluidic Autologous Serum Eye-Drops Preparation as a Potential Dry Eye Treatment. 国際誌

    Yasui T, Morikawa J, Kaji N, Tokeshi M, Tsubota K, Baba Y

    Micromachines   7 巻 ( 7 ) 頁: 113 - 113   2016年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Micromachines  

    Dry eye is a problem in tearing quality and/or quantity and it afflicts millions of persons worldwide. An autologous serum eye-drop is a good candidate for dry eye treatment; however, the eye-drop preparation procedures take a long time and are relatively troublesome. Here we use spiral microchannels to demonstrate a strategy for the preparation of autologous serum eye-drops, which provide benefits for all dry eye patients; 100% and 90% removal efficiencies are achieved for 10 μm microbeads and whole human blood cells, respectively. Since our strategy allows researchers to integrate other functional microchannels into one device, such a microfluidic device will be able to offer a new one-step preparation system for autologous serum eye-drops.

    DOI: 10.3390/mi7070113

    Web of Science

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    PubMed

  23. A Japanese multicenter survey characterizing pain in Parkinson's disease

    Kubo S.I., Hamada S., Maeda T., Uchiyama T., Hashimoto M., Nomoto N., Kano O., Takahashi T., Terashi H., Takahashi T., Hatano T., Hasegawa T., Baba Y., Sengoku R., Watanabe H., Kadowaki T., Inoue M., Kaneko S., Shimura H., Nagayama H.

    Journal of the Neurological Sciences   365 巻   頁: 162 - 166   2016年6月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Journal of the Neurological Sciences  

    Background Pain is a frequent, troublesome symptom of PD but is under-recognized and poorly understood. Aim We characterized pain prevalence, severity, and location in PD, to better understand its pathophysiology and improve diagnosis and treatment. Subjects and methods A cross-sectional controlled study was conducted at 19 centers across Japan. A total of 632 subjects with Mini-Mental State Examination scores ≥ 24 were enrolled, including 324 PD patients and 308 controls. Sex and mean age did not differ between the two groups. Demographic and clinical data were collected. Pain was assessed using questionnaires, the SF-36v2 bodily pain scale, and a body illustration for patients to indicate the location of pain in 45 anatomical areas. Results Pain prevalence in the PD group was 78.6%, significantly higher than in controls (49.0%), as was its severity. There was no correlation between SF-36v2 score and motor scores, such as Unified Parkinson's Disease Rating Scale III or Hoehn & Yahr scores. Pain distribution was similar between groups, predominantly in the lower back, followed by the gluteal region, lower legs, thighs, posterior neck, and shoulders. Conclusion Pain is a significant problem in the Japanese PD population and we discuss its pathophysiology.

    DOI: 10.1016/j.jns.2016.04.015

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  24. Sialylation converts arthritogenic IgG into inhibitors of collagen-induced arthritis

    Ohmi Y., Ise W., Harazono A., Takakura D., Fukuyama H., Baba Y., Narazaki M., Shoda H., Takahashi N., Ohkawa Y., Ji S., Sugiyama F., Fujio K., Kumanogoh A., Yamamoto K., Kawasaki N., Kurosaki T., Takahashi Y., Furukawa K.

    Nature Communications   7 巻   2016年4月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nature Communications  

    Rheumatoid arthritis (RA)-associated IgG antibodies such as anti-citrullinated protein antibodies (ACPAs) have diverse glycosylation variants; however, key sugar chains modulating the arthritogenic activity of IgG remain to be clarified. Here, we show that reduced sialylation is a common feature of RA-associated IgG in humans and in mouse models of arthritis. Genetically blocking sialylation in activated B cells results in exacerbation of joint inflammation in a collagen-induced arthritis (CIA) model. On the other hand, artificial sialylation of anti-type II collagen antibodies, including ACPAs, not only attenuates arthritogenic activity, but also suppresses the development of CIA in the antibody-infused mice, whereas sialylation of other IgG does not prevent CIA. Thus, our data demonstrate that sialylation levels control the arthritogenicity of RA-associated IgG, presenting a potential target for antigen-specific immunotherapy.

    DOI: 10.1038/ncomms11205

    Web of Science

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  25. A high-sensitivity cyclodextrin-microchip electrophoresis simultaneous analysis of gabapentin and pregabalin

    Zeid A., Nasr J., Kaji N., Belal F., Walash M., Baba Y.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016     頁: 1457 - 1458   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016  

    We report a highly-sensitive microchip electrophoretic method for the simultaneous analysis of gabapentin (GPN) and pregabalin (PGN). Analyses and separation of both compounds were achieved in a PMMA microchip after labelling with 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). The separation of the labelled compounds was improved by addition of β-cyclodextrin (β-CD). Moreover, methylcellulose polymer was added to prevent analytes' adsorption on the microchannel walls. The sensitivity of the method was improved by stacking to quantify as low as 0.06 nM of each drug with detection limits of 12 pM.

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  26. Effects of the grooved structures and the ethanol concentration on the small-sized lipid nanoparticles formation

    Fujishima Y., Maeki M., Sato Y., Yasui T., Ishida A., Tani H., Baba Y., Harashima H., Tokeshi M.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016     頁: 1412 - 1413   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016  

    This paper describes lipid nanoparticles (LNPs) formation behavior in microfluidic devices equipped with the staggered herringbone micromixers (SHM) in order to control the LNPs size precisely. Three types of microfluidic devices with different heights of mixer structures were fabricated to examine the effect of mixer structure on the LNPs formation behavior. We found the height of the grooved structures, the lipid concentration, and the ethanol concentration were significant factors for controlling LNPs size and its distribution.

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  27. Water film device for continuous particulate matter collection

    Shimada T., Yasui T., Hibara A., Yanagida T., Kaji N., Kanai M., Nagashima K., Kawai T., Baba Y.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016     頁: 1473 - 1474   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016  

    For collection of particulate matter (PM) contained in gas phase, we fabricated water film device to transfer PM2.5 from gas to liquid phase, continuously. The device had millimeter-scale channel and long-term stable superhydrophilic surface formed by titanium oxide (TiO2)-covered zinc oxide (ZnO) nanowires to make a parallel flow of gas and liquid phases. The superhydrophilic surface al-lowed to form stable liquid phase within the channel and collect PM2.5 from gas phase. This device would be a new platform for collecting harmful PM from the air and keeping air condition clean.

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  28. Simultaneous ionic current and optical detection of fine particulate matters (PM2.5) based on cross-junction microfluidic device

    Rahong S., Yasui T., Yasaki H., Yanagida T., Kanai M., Nagashima K., Kaji N., Kawai T., Baba Y.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016     頁: 1284 - 1285   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016  

    We present a simultaneous electrical and optical detection of particulate matter (PM) based on microfluidic chip for label-free and fast detection. The microfluidic system consists of the filters area for screening the large particles, which allows to pass through only particles smaller than 2.5μm, and the sensing area for detecting the particles using DC electrical measurements. As PM2.5 passed the cross-junction area, the disturbing electric fields would lead to fluctuating electric current in the detection circuit unit. Our measurement system has dynamic range from 200 nm to 2.5 μm. Furthermore, the amplitudes and the dwelling time of signals depended on the size of particulate matters that mean we would discriminate PM2.5 size in the range of millisecond (1-10 ms). Our system can detect a single particulate matter, which is applicable to count the number of PM2.5 for medical diagnostic, since the number of PM2.5 are found to be associated with several diseases.

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  29. Self-assembled monolayer and peptide coated nanowire devices for selective detection of C-reactive protein and E.coli without antibody

    Yokoyama A., Yasui T., Goda T., Yanagida T., Tanaka M., Muto M., Okochi M., Kaji N., Kanai M., Nagashima K., Miyahara Y., Kawai T., Baba Y.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016     頁: 1156 - 1157   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016  

    We developed surface-coated nanowire device with 2-methacryloyloxyethyl phosphorylcholine self-assembled monolayer for the detection of C-reactive protein (CRP) and phage display screened specific peptide for the detection of Escherichia coli (E. coli) without antibody. In this work, for achieving high-efficient target capturing we focused on combination of nanostructure and surface coating. We fabricated zinc oxide (ZnO) nanowire devices by hydrothermal synthesis and coated their surface with materials recognizing each biomarker without antibody. 3D structure of nanowire and surface coating enhanced target capture ability.

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  30. Recognition micropores for detecting single-bacteria

    Tsutsui M., Yokota K., Yasui T., Yasaki H., Okochi M., Taniguchi M., Washio T., Baba Y., Kawai T.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016     頁: 816 - 817   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016  

    We report on development of a bio-inspired micropore sensor for detecting and discriminating single-bacteria selectively by leveraging the receptor specificity to recognize a target bioparticle upon its translocation. A feasibility study showed that by decorating a pore wall surface with an engineered peptide molecule, the translocation dynamics of certain types of bacteria could be retarded substantially thereby facilitating the discrimination via the resistive pule analysis. The recognition pore sensing presented here is essentially applicable to not only bacterial screening but also for many other application including viral detections and proteomics by making pore size to fit an analyte of interest.

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  31. Nanowires for early cancer and diabetes diagnosis via micro-RNA detection in urine extracellular vesicles

    Yasui T., Takeshita D., Yanagida T., Kaji N., Kanai M., Nagashima K., Yukawa H., Kawai T., Baba Y.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016     頁: 108 - 109   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016  

    Inorganic nanowires recently show their great promise for analyzing cells or intracellular components due to the nanowires feature of a stealth effect against cell surfaces [1]. Here we showed a novel usability of the nanowires to achieve an efficient capture of extracellular vesicles, which surfaces is similar to the cell surfaces, and early diagnosis based on the vesicles microRNAs. Our methodology could achieve extracellular vesicles capture in urine over 95% within 40 min, and detect cancer- and type 2 diabetes-related microRNAs from urine samples.

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  32. Millisecond measurement of single cell size and deformability by electrical detection in microfluidic device with two consecutive constrictions

    Kaji N., Sano M., Yasaki H., Yasui T., Baba Y.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016     頁: 341 - 342   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016  

    A mechanical property of a cell, cell deformability, was measured by two consecutive constrictions in a microchannel by the electrical current measurement system. Thanks to the first constriction for cell size measurement, cell deformability was precisely measured considering a cell size itself and the effects of cytoskeleton-affecting drugs were detected within hundreds milliseconds.

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  33. Microfluidic long-pore-channel to highlight bacteria contents

    Yasaki H., Yasui T., Yanagida T., Kaji N., Kanai M., Fukuyama M., Nagashima K., Kawai T., Baba Y.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016     頁: 100 - 101   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016  

    Ionic current sensing has realized sizing and counting of the number of biological samples, however, the sensing has never discriminated sample species in principle. Here, we showed the first case reported to date for discriminating sample species using microfluidic long-pore channel. The microfluidic long-pore channel could detect sample size, count sample number, and discriminate breaking of cell membrane of samples during sample passing through the long-pore channel. The present new approach should allow to develop an analytical tool not only for sizing and counting but for discriminating biological samples.

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  34. Massively parallel single nuclei assay on microchamber array devices

    Ito S., Kaji N., Yasui T., Baba Y.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016     頁: 679 - 680   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016  

    We have developed microchamber array device with 10,000 microchambers for real time analysis of RNA transportation through nuclear pore complex (NPC). The exported messenger ribonucleic acid (mRNA) successfully observed through the experiments of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA detection over 150 mins.

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  35. Immuno-wall lab-on-a-chip protein analysis devices for high precision surgery of gliomas

    Kasama T., Yamamichi A., Ohka F., Kato Y., Suzuki H., Kato A., Motomura K., Hirano M., Ranjit M., Chalise L., Kurimoto M., Kondo G., Aoki K., Kaji N., Matsubara T., Suzuki H., Tokeshi M., Wakabayashi T., Natsume A., Baba Y.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016     頁: 651 - 652   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016  

    In the present study, we propose immuno-wall lab-on-a-chip protein analysis devices for ultrarapid detection of R132H mutant isocitrate dehydrogenase 1 (IDH1) that we identified as a specifically and commonly expressed gene in diffusely infiltrative gliomas1. In 9 min immunoassay, we successfully carried out specific detection of R132H mutant IDH1 in a lysate of glioma tissue, which is much faster than intraoperative pathological diagnosis. Moreover, clinical samples from 10 patients with gliomas were analyzed by using the immuno-wall devices. These results were consistent with those of PCR-based genetic mutation detection method. Our devices realize point-of-care tumor testing, resulting in the high precision surgery of gliomas.

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  36. Hepatic arterial infusion chemotherapy with fine-powder cisplatin and iodized-oil suspension in patients with intermediate-stage and advanced-stage (Barcelona Clinic Liver Cancer stage-B or stage-C) hepatocellular carcinoma: multicenter phase-II clinical study

    Takaki H., Yamakado K., Tsurusaki M., Yasumoto T., Baba Y., Narimatsu Y., Shimohira M., Yamaguchi M., Matsuo K., Inaba Y., Mikami K., Watanabe R., Nishida N., Anai H., Kakizawa H., Hirota S.

    International Journal of Clinical Oncology   20 巻 ( 4 ) 頁: 745 - 754   2015年8月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:International Journal of Clinical Oncology  

    Purpose: This single-arm, multicenter, phase-II trial evaluated the efficacy and safety of hepatic arterial infusion chemotherapy (HAIC) using fine-powder cisplatin and iodized-oil suspension in patients with intermediate- and advanced-stage [Barcelona Clinic Liver Cancer (BCLC) stage-B and stage-C] hepatocellular carcinomas (HCCs). Methods: The Institutional Review Board approved this study and patients provided written informed consent. Thirty-five patients (24 men and 11 women, mean 74 ± 6 years [range 60–87 years]) with BCLC stage-B (57 %, 20/35) or stage-C (43 %, 15/35) HCCs who were not candidates for other locoregional treatments were enrolled. HAIC was performed using a suspension of fine-powder cisplatin with a maximum dose of 65 mg/m2 and iodized oil on demand. The primary endpoint was the response rate evaluated based on Response Evaluation Criteria in Solid Tumor (RECIST) and modified RECIST (mRECIST). Secondary endpoints were overall survival, progression-free survival, and safety. Results: The initial and best overall response rates at 4 weeks and 3 months, respectively, were 14 and 17 % based on RECIST, and 57 and 23 % based on mRECIST. The median overall and progression-free survival times were 18 and 4 months, respectively. The most frequent grade-3 or grade-4 adverse events were elevation of serum alanine (23 %) and aspartate aminotransferase (20 %), and thrombocytopenia (17 %). Conclusion: This HAIC provides promising therapeutic effects with acceptable safety to patients with intermediate-stage and advanced-stage HCCs.

    DOI: 10.1007/s10147-014-0773-4

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  37. Nanobiodevice-based single cell analysis and single biomolecule analysis

    Yasui T., Baba Y.

    Bunseki Kagaku   64 巻 ( 6 ) 頁: 413 - 419   2015年7月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Bunseki Kagaku  

    A nanobiodevice is a piece of contrivance, equipment, machine, or component, which is created by overlapping multidisciplinary activities associated with nanotechnology and biotechnology, intended for biological, medical, and clinical purposes. We developed nanobiodevices for biomedical applications, including single cancer-cell diagnosis for cancer metastasis, circulating tumor cell CTC. detection by microfluidic devices, nanopillar devices for the ultrafast analysis of genomic DNA and microRNA, nanopore devices for single DNA and microRNA sequencing, nanowire devices for exosome analysis, single-molecular epigenetic analysis, quantum switching in vivo imaging of iPS cells and stem cells, and quantum technology-based cancer theranostics.

    DOI: 10.2116/bunsekikagaku.64.413

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  38. Three-dimensional Nanowire Structures for Ultra-Fast Separation of DNA, Protein and RNA Molecules.

    Rahong S, Yasui T, Yanagida T, Nagashima K, Kanai M, Meng G, He Y, Zhuge F, Kaji N, Kawai T, Baba Y

    Scientific reports   5 巻   頁: 10584   2015年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Scientific Reports  

    Separation and analysis of biomolecules represent crucial processes for biological and biomedical engineering development; however, separation resolution and speed for biomolecules analysis still require improvements. To achieve separation and analysis of biomolecules in a short time, the use of highly-ordered nanostructures fabricated by top-down or bottom-up approaches have been proposed. Here, we reported on the use of three-dimensional (3D) nanowire structures embedded in microchannels fabricated by a bottom-up approach for ultrafast separation of small biomolecules, such as DNA, protein, and RNA molecules. The 3D nanowire structures could analyze a mixture of DNA molecules (50-1000 bp) within 50 s, a mixture of protein molecules (20-340 kDa) within 5 s, and a mixture of RNA molecules (100-1000 bases) within 25 s. And, we could observe the electrophoretic mobility difference of biomolecules as a function of molecular size in the 3D nanowire structures. Since the present methodology allows users to control the pore size of sieving materials by varying the number of cycles for nanowire growth, the 3D nanowire structures have a good potential for use as alternatives for other sieving materials.

    DOI: 10.1038/srep10584

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  39. Arrangement of a nanostructure array to control equilibrium and nonequilibrium transports of macromolecules.

    Yasui T, Kaji N, Ogawa R, Hashioka S, Tokeshi M, Horiike Y, Baba Y

    Nano letters   15 巻 ( 5 ) 頁: 3445 - 51   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nano Letters  

    Exploiting the nonequilibrium transport of macromolecules makes it possible to increase the separation speed without any loss of separation resolution. Here we report the arrangement of a nanostructure array in microchannels to control equilibrium and nonequilibrium transports of macromolecules. The direct observation and separation of macromolecules in the nanopillar array reported here are the first to reveal the nonequilibrium transport, which has a potential to overcome the intrinsic trade-off between the separation speed and resolution.

    DOI: 10.1021/acs.nanolett.5b00783

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  40. A strategy for synthesis of lipid nanoparticles using microfluidic devices with a mixer structure 国際誌

    Maeki Masatoshi, Saito Tatsuyoshi, Sato Yusuke, Yasui Takao, Kaji Noritada, Ishida Akihiko, Tani Hirofumi, Baba Yoshinobu, Harashima Hideyoshi, Tokeshi Manabu

    RSC ADVANCES   5 巻 ( 57 ) 頁: 46181 - 46185   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:RSC Advances  

    Formation behavior of lipid nanoparticles (LNPs) in microfluidic devices with a staggered herringbone micromixer (SHM) structure was investigated. The fundamental role for SHMs in LNP formation was demonstrated by determining such factors as the limiting SHM cycle numbers and the effect of flow rate. The SHM cycle numbers and the position of the first SHM were as significant as factors as the flow rate condition for producing the small-size LNPs.

    DOI: 10.1039/c5ra04690d

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  41. Self-assembled nanowire arrays as three-dimensional nanopores for filtration of DNA molecules.

    Rahong S, Yasui T, Yanagida T, Nagashima K, Kanai M, Meng G, He Y, Zhuge F, Kaji N, Kawai T, Baba Y

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   31 巻 ( 3 ) 頁: 153 - 7   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Sciences  

    Molecular filtration and purification play important roles for biomolecule analysis. However, it is still necessary to improve efficiency and reduce the filtration time. Here, we show self-assembled nanowire arrays as three-dimensional (3D) nanopores embedded in a microfluidic channel for ultrafast DNA filtration. The 3D nanopore structure was formed by a vapor-liquid-solid (VLS) nanowire growth technique, which allowed us to control pore size of the filtration material by varying the number of growth cycles. λ DNA molecules (48.5 kbp) were filtrated from a mixture of T4 DNA (166 kbp) at the entrance of the 3D nanopore structure within 1 s under an applied electric field. Moreover, we observed single DNA molecule migration of T4 and λ DNA molecules to clarify the filtration mechanism. The 3D nanopore structure has simplicity of fabrication, flexibility of pore size control and reusability for biomolecule filtration. Consequently it is an excellent material for biomolecular filtration.

    DOI: 10.2116/analsci.31.153

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  42. Superhydrophilic glass membrane device with open-microhole array for filtering and counting rare tumor cells

    Yonese A., Onoshima D., Yukawa H., Ishikawa K., Hori M., Baba Y.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 493 - 495   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    We report the development of a glass membrane filter device with open microholes for size-based isolation with direct on-chip characterization of rare tumor cells at a single cell level. The enhanced wettability by exposure to an atmospheric plasma enabled cell suspension to flow through the holes and single tumor cells were successfully trapped inside the holes. Different plasma-exposure amounts and directions were studied to evaluate the trapping efficiency of the device for optimizing the condition of device. This device can be applied to medical diagnosis of cancer metastasis in the future.

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  43. A microchamber array device for studying genomic diversity in cancer cells at a single cell level

    Ito S., Kaji N., Yasui T., Baba Y.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 534 - 536   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    We have developed microchamber array device for direct quantitative analysis of messenger ribonucleic acids (mRNA) inside a single cell with two types of fluorescent probes. Genomic diversity in HeLa cancer cells at a single cell level was successfully observed through the experiments of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA detection and monitoring over 3 hours.

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  44. Cell deformability measurements for single cancer cells by ionic current in microfluidic devices

    Sano M., Kaji N., Yasui T., Baba Y.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 455 - 457   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    To realize high-throughput, easy to use, and high-accuracy cell deformability measurements, we have developed ionic current detection system combined with constriction structure inside microchannel. In this system, when cells are clogged in constriction, the resistivity of detection area increases and the current decreases by ionic current blockade. We successfully detected unique signal shapes derived from cell deformability during the cell passage at the constriction. The signal shapes might provide an information on cell deformation and nucleus position inside a cell.

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  45. A single cell nucleus array to monitor messenger-RNA molecule transportation through nuclear membrane

    Kaji N., Koyama R., Yasui T., Higashiyama T., Baba Y.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 832 - 834   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    In this study, a microchamber array device was designed to capture a single cell, lyse a cell membrane, and isolate a single cell nucleus, and then mRNA in a single cell nucleus were directly quantitated by using highly sensitive In-Stem Molecular Beacons (ISMB). As a result, the expression of three frequently used house-keeping genes were successfully quantitated to an accuracy of 100 molecules within a single cell nucleus.

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  46. Conformation and dynamic behavior of single DNA molecules in nanofluidic channels for detection of DNA methylation

    Sun X., Yasui T., Rahong S., Yanagida T., Kaji N., Kanai M., Nagashima K., Kawai T., Baba Y.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 1115 - 1117   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    In this paper, the single DNA molecules were linearized and stretched by using the nanochannel with a dimension of about 300 × 300 nm2 under applying electric fields. After that, the single DNA molecules methylation level was detected by estimating the contraction processes of both non-methylated and methylated single DNA molecules in nanochannel after switching off the electric fields. And furthermore, we also found that their translocation velocities were changed between non-methylated and methylated single DNA molecules in nanochannel.

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  47. Pdms-anchored nanowires for high throughput micro-RNA extraction from extracellular vesicles in body fluid

    Takeshita D., Yasui T., Yong H., Yanagida T., Kaji N., Kanai M., Nagashima K., Kawai T., Baba Y.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 1516 - 1518   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    We anchored zinc oxide nanowires (ZnO) in PDMS microchannel for high throughput capture of extracellular vesicles and microRNA extraction in them from body fluids. Using this device, we achieved vesicles capture from urine sample within 10 min, which is faster than using the conventional methods, such as precipitation kit or ultracentrifugation. And we demonstrated microRNA extraction from the captured vesicles. These results highlighted that we realized high throughput microRNA extraction from body fluid.

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  48. Nanowire lysis and DNA extracrtion from a single bacterium for bacteria analysis

    Yasui T., Otsuka K., Takeuchi M., Yanagida T., Kaji N., Kanai M., Rahong S., Nagashima K., Naito T., Kawai T., Baba Y.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 320 - 322   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    Exploiting the method to extract DNA molecules from bacteria makes it possible to analyze bacteria in a short time. Here, we realized DNA extraction from a single bacterium via physical interactions between nanowires and bacteria; nanowire lysis. The combination of nanowire lysis and heating at 94°C could help us to extract genome DNA from bacteria, efficiently.

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  49. Nanowire devices for extracellular vesicles analysis towards elucidation of intercellular communication

    Tabuchi K., Yasui T., Yong H., Yanagida T., Kaji N., Kanai M., Nagashima K., Kawai T., Baba Y.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 1510 - 1512   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    We fabricate ZnO nanowires inside microchannels on Si substrates. Since the ZnO nanowires have positively charged surface [1] and most vesicles have negatively charged surface, it is able to capture the vesicles on the nanowire surface by electrostatic interaction. MicroRNA is extracted from the captured vesicles by introducing lysis buffer. It is expected that our fabricate devices would lead to fundamental understanding of cell-cell communication.

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  50. Microfluidic approach for production of lipid nanoparticles-based nano medicine

    Maeki M., Saito T., Node Y., Sato Y., Yasui T., Kaji N., Ishida A., Tani H., Baba Y., Harashima H., Tokeshi M.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 838 - 840   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    This paper described a simple preparation method for small-size and monodispersed lipid nanoparticles (LNPs) by using microfluidic devices. The fundamental role and importance of chaotic micromixer in the microfluidic device was demonstrated. The suitable cycle number of chaotic micromixer was confirmed for precise controlling LNPs size with narrow distribution under the any flow rate conditions. In addition, LNPs containing siRNA was synthesized for evaluation of penetration efficiency via in vivo experiment. The PEGylated LNPs containing siRNA with a diameter of 30 nm could penetrate to the mouse parenchymal liver cells rather than the LNPs with a diameter of 50 nm.

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  51. Label-free detection of extracellular vesicles for cancer diagnosis

    Ajiri T., Yasui T., Ishida A., Tani H., Baba Y., Tokeshi M.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 1789 - 1791   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    We have developed a label-free detection method of biomolecules using nanostructures [1]. The principle of this method is based on intensity changes of diffracted light derived from the nanostructures. This method is very simple and useful for label-free detection of biomolecules, but further improvement in sensitivity was necessary to apply it to clinical applications. In this paper, we optimized optical system and device design to improve the sensitivity, and applied it to measure extracellular vesicles for cancer diagnosis. These results showed that our method had a potential to be a first screening method for cancer diagnosis.

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  52. Immuno-wall lab-on-chip companion diagnostic devices for rapid and low-cost detection of mutant epidermal growth factor receptors (EGFR) from cytological samples in lung cancer patients

    Kasama T., Hase T., Nishiwaki N., Yogo N., Sato M., Kondo M., Kaji N., Tokeshi M., Hasegawa Y., Baba Y.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 925 - 927   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    In the present study, we propose immuno-wall lab-on-a-chip companion diagnostic devices for epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKI) such as erlotinib and gefitinib. The lysates of cytological samples including pleural effusion in lung cancer patients were successfully analyzed within 20 minutes. This is the first experiment demonstrating the detection of mutated EGFRs in the pleural effusion by microdevices. Our devices have a great potential to become the next generation companion diagnostic devices which overcome the problems of currently available methods.

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  53. Highly sensitive ionic current sensing system with optical observation for discriminating a wide diversity of sizes of bacteria with contaminants

    Yasaki H., Yasui T., Rahong S., Yanagida T., Kaji N., Kanai M., Nagashima K., Kawai T., Baba Y.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 314 - 316   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    We realized highly sensitive ionic current sensing system with optical observation for discriminating a wide diversity of sizes of bacteria (0.15∼10 μm) in biological samples with many contaminants. The proposed system enables us to discriminate 1% size difference of each sample within 400 ms. This highly sensitive ionic current sensing system with optical observation might provide a new sensing scheme to detect a single bacteria based on their sizes, shapes, colors, and stainability.

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  54. High-throughput methylation mapping by detecting fluorescently stained methylation sites at a single molecule level

    Hattori A., Yasui T., Kaji N., Baba Y.

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 861 - 863   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

    We detected methylation sites on elongated lambda DNA molecules as high fluorescent distinct peaks via double collar imaging. We used methyl-CpG-binding domain 2 protein (MBD2p) combined with the quantum dots (QDs) for methylation imaging, and YOYO-1 for DNA imaging.

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  55. Rhinorrhea in Parkinson's disease: A consecutive multicenter study in Japan

    Kano O., Yoshioka M., Nagayama H., Hamada S., Maeda T., Hasegawa T., Kadowaki T., Sengoku R., Terashi H., Hatano T., Nomoto N., Inoue M., Shimura H., Takahashi T., Uchiyama T., Watanabe H., Kaneko S., Takahashi T., Baba Y., Kubo S.I.

    Journal of the Neurological Sciences   343 巻 ( 1-2 ) 頁: 88 - 90   2014年8月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Journal of the Neurological Sciences  

    Recent reports suggest that rhinorrhea, defined as the presence of a runny nose unrelated to respiratory infections, allergies, or sinus problems, occurs more frequently among patients with Parkinson's disease (PD) than among healthy controls. We conducted a questionnaire survey in a multicenter study throughout Japan and compared the frequency of rhinorrhea between 231 PD and 187 normal control (NC) subjects. After excluding patients with rhinitis or paranasal sinusitis, a total of 159 PD and 59 NC subjects were included in our analysis. Rhinorrhea occurred more frequently in PD patients than NC subjects (33.3% vs. 11.9%; P = 0.01). Among PD patients, rhinorrhea was more common in men than women (P = 0.005). Rhinorrhea was not correlated with disease duration, modified Hoehn and Yahr score, disease type (akinesia rigidity vs. tremor dominant), or cardiac sympathetic function (evaluated by 123I- metaiodobenzylguanidine uptake). To our knowledge, this is the first multicenter study on the frequency of PD-related rhinorrhea in Asian countries. © 2014 Elsevier B.V.

    DOI: 10.1016/j.jns.2014.05.039

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  56. Hepatic Monoacylglycerol O-acyltransferase 1 as a Promising Therapeutic Target for Steatosis, Obesity, and Type 2 Diabetes.

    Hayashi Y, Suemitsu E, Kajimoto K, Sato Y, Akhter A, Sakurai Y, Hatakeyama H, Hyodo M, Kaji N, Baba Y, Harashima H

    Molecular therapy. Nucleic acids   3 巻   頁: e154   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Molecular Therapy - Nucleic Acids  

    Over the past decade, considerable advances have been made in the discovery of gene targets in metabolic diseases. However, in vivo studies based on molecular biological technologies such as the generation of knockout mice and the construction of short hairpin RNA vectors require considerable effort and time, which is a major limitation for in vivo functional analysis. Here, we introduce a liver-specific nonviral small interfering RNA (siRNA) delivery system into rapid and efficient characterization of hepatic gene targets in metabolic disease mice. The comparative transcriptome analysis in liver between KKAy diabetic and normal control mice demonstrated that the expression of monoacylglycerol O-acyltransferase 1 (Mogat1), an enzyme involved in triglyceride synthesis and storage, was highly elevated during the disease progression. The upregulation of Mogat1 expression in liver was also found in other genetic (db/db) and diet-induced obese mice. The silencing of hepatic Mogat1 via a liver-specific siRNA delivery system resulted in a dramatic improvement in blood glucose levels and hepatic steatosis as well as overweight with no apparent overall toxicities, indicating that hepatic Mogat1 is a promising therapeutic target for metabolic diseases. The integrated approach with transcriptomics and nonviral siRNA delivery system provides a blueprint for rapid drug discovery and development.

    DOI: 10.1038/mtna.2014.4

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  57. Microfluidic biosensor for the detection of DNA by fluorescence enhancement and the following streptavidin detection by fluorescence quenching.

    Wang J, Aki M, Onoshima D, Arinaga K, Kaji N, Tokeshi M, Fujita S, Yokoyama N, Baba Y

    Biosensors & bioelectronics   51 巻   頁: 280 - 5   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Biosensors and Bioelectronics  

    We reported an optical DNA/protein microfluidic sensor which consists of single stranded (ss) DNA-Cy3 probes on gold surface and simple line-shape microfluidic channel. These ssDNA-Cy3 probes with random sequence in bulk solution or on gold surface exhibits fluorescence enhancement after binding with complementary ssDNA (cssDNA) targets. Particularly it did not require complicated design or hairpin-like stem-loop conformation, which made it easier to be made and applied in analytes detection by fluorescence switching techniques. Using ssDNA-cy3 probes attached on gold surface in a microfluidic channel, strong fluorescence enhancement was measured by ssDNA with cssDNA binding or ssDNA with cssDNA-biotin binding. The following introduction of streptavidin resulted in fluorescence quenching (fluorescence decrease) because of the binding of hybridized DNA-biotin with streptavidin. This sensor showed strong affinity and high sensitivity toward the streptavidin, the minimum detectable concentration for streptavidin was 1. pM, equating to an absolute detection limit of 60. amol in this microfluidic channel. Microfluidic channel height and flow rate is optimized to increase surface reaction efficiency and fluorescence switching efficiency. In contrast to previously reported optical molecular beacon approach, this sensor can be used not only for the detection of cssDNA target, but also for the detection of streptavidin. This microfluidic sensor offers the promise of analyzing kinds of molecular targets or immunoreactions. © 2013 Elsevier B.V.

    DOI: 10.1016/j.bios.2013.07.058

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  58. Nationwide post event survey and analysis of the 2011 Tohoku earthquake tsunami

    Mori N., Takahashi T., Hamaura S.E., Miyakawa K., Tanabe K., Tanaka K., Tanaka M., Watanabe T., Matsutomi H., Naoe K., Noumi T., Yamaguchi E., Ando S., Fujii Y., Kashima T., Okuda Y., Shibazaki B., Sakakiyama T., Matsuyama M., Yoshii T., Goto K., Aida T., Kurata Y., Miyazaki M., Shutou K., Suzuki J., Takeuchi H., Nakamura T., Fujiwara O., Kagohara K., Kimura H., Komatsubara J., Miyashita Y., Namegaya Y., Sawai Y., Shishikura M., Tanigawa K., Fritz H., Uzaki K.I., Sasaki M., Minami M., Endou H., Hashimoto M., Kobashigawa Y., Kumagai M., Ietsune M., Nakamura K., Gusman A., Hirakawa K., Loki K., Nakamura Y., Nakayama T., Nishimura Y., Putra P., Saruwatari A., Watanabe Y., Yamada T., Nabetani Y., Nobuoka H., Tamada T., Matsubayashi Y., Ogasawara T., Sakai S., Abe M., Hayashi Y., Iino H., Iwakiri K., Kimura K., Maeda K., Okada M., Tsushima H., Kakinuma T., Yamashita K., Umeda S., Nakamura T., Seto S., Takahiro K., Torayashiki T., Tsujimoto G., Uno K., Yoshioka S., Dewa N., Hayashi T., Kitamura M., Kuroda S., Nakahira A., Nozawa T., Taniwaki K., Ohtoshi K., Aoyama T., Chiba T., Enomoto H., Hirahara K., Murai S., Narayama H., Yamanaka S., Yamazaki H., Yoshiiri S., Azuma R., Baba Y.

    Coastal Engineering Journal   54 巻 ( 1 )   2014年1月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Coastal Engineering Journal  

    At 14:46 local time on March 11, 2011, a magnitude 9.0 earthquake occurred off the coast of northeast Japan. This earthquake generated a tsunami that struck Japan as well as various locations around the Pacific Ocean. With the participation of about 300 researchers from throughout Japan, joint research groups conducted a tsunami survey along a 2,000 km stretch of the Japanese coast. More than 5,200 locations have been surveyed to date, generating the largest tsunami survey dataset in the world. The inundation height and run-up height were surveyed by laser, GPS, and other instruments, and the tidal correction has been accurately adjusted using a tidal database and a numerical simulation for Tohoku, an area where tide gauges were destroyed by the tsunami. Based on the survey dataset, the regional and local scale analyses were conducted to understand the basic characteristics of this event. Maximum run-up heights greater than 10 m are distributed along 500 km of coast in direct distance. The affected area of this event was several times larger than historically recorded in Tohoku. The mean inundation height in the southern Sanriku region is 1015 m and there are several peaks of inundation along the coast from the northern to middle part of Sanriku. © 2012 World Scientific Publishing Company and Japan Society of Civil Engineers.

    DOI: 10.1142/S0578563412500015

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  59. Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells.

    Yukawa H, Nakagawa S, Yoshizumi Y, Watanabe M, Saito H, Miyamoto Y, Noguchi H, Oishi K, Ono K, Sawada M, Kato I, Onoshima D, Obayashi M, Hayashi Y, Kaji N, Ishikawa T, Hayashi S, Baba Y

    PloS one   9 巻 ( 11 ) 頁: e110142   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PLoS ONE  

    Stem cell transplantation has been expected to have various applications for regenerative medicine. However, in order to detect and trace the transplanted stem cells in the body, non-invasive and widely clinically available cell imaging technologies are required. In this paper, we focused on magnetic resonance (MR) imaging technology, and investigated whether the trimethylamino dextran-coated magnetic iron oxide nanoparticle -03 (TMADM-03), which was newly developed by our group, could be used for labeling adipose tissue-derived stem cells (ASCs) as a contrast agent. No cytotoxicity was observed in ASCs transduced with less than 100 μg-Fe/mL of TMADM-03 after a one hour transduction time. The transduction efficiency of TMADM-03 into ASCs was about four-fold more efficient than that of the alkali-treated dextrancoated magnetic iron oxide nanoparticle (ATDM), which is a major component of commercially available contrast agents such as ferucarbotran (Resovist), and the level of labeling was maintained for at least two weeks. In addition, the differentiation ability of ASCs labeled with TMADM-03 and their ability to produce cytokines such as hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF) and prostaglandin E2 (PGE2), were confirmed to be maintained. The ASCs labeled with TMADM-03 were transplanted into the left kidney capsule of a mouse. The labeled ASCs could be imaged with good contrast using a 1T MR imaging system. These data suggest that TMADM-03 can therefore be utilized as a contrast agent for the MR imaging of stem cells.

    DOI: 10.1371/journal.pone.0110142

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  60. Nanobiodevice-based single biomolecule analysis, single-cell analysis, and in vivo imaging for cancer diagnosis, cancer theranostics, and iPS cell-based regenerative medicine.

    Kaji N, Baba Y

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   30 巻 ( 9 ) 頁: 859 - 64   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Sciences  

    Numerous nanobiodevices have been developed for cancer diagnosis through the analysis of cancer cells and cancer-related biomolecules, cancer gene therapy, and iPS cell-based regenerative medicine. A microchamber array, which is fabricated on a plastic chip, enables to develop a reliable circulating tumor cell (CTC) detection technique for cancer metastasis diagnosis. A nanopillar-array or a nanowire-array on a quartz chip allows ultrafast analysis of DNA and microRNA within 1 s for the molecular diagnosis of cancer. Immunopillar devices, which contain a tremendous amount of antibody-immobilized microparticles inside an immunopillar structure, have realized fast and low invasive "from blood to analysis" type biomarker detection of cancer with a pM detection sensitivity within 5 min. Quantum dots and gene delivery nanodevices are applied to single cancer cell diagnosis, in vivo imaging for iPS cell based regenerative medicine, and theranostic devices for cancer diagnosis and therapy.

    DOI: 10.2116/analsci.30.859

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  61. A millisecond micro-RNA extraction technique for nanopore-based nucleic acid sequencing

    Wu Q., Yasui T., Rahong S., Yanagida T., Kanai M., Kaji N., Tokeshi M., Nagashima K., Kawai T., Baba Y.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014     頁: 233 - 235   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014  

    A ms(millisecond) miRNA(micro RNA) extraction from a mixture of total RNA and genomic DNA was successfully achieved by the combination of nanopillar and nanoslit structures inside a microchannel. This ultra-fast miRNA extraction technique especially useful for exosome-derived miRNA sequencing by nanopore-based RNA sequencer since exosomes contain miRNA and mRNA.

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  62. Additive manufacturing based on injection molding for three dimensional microfluidics

    Naito T., Nakamura M., Kubo T., Yasui T., Kaji N., Baba Y., Otuska K.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014     頁: 1686 - 1688   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014  

    This paper describes three dimensional (3D) microfluidic devices fabricated by an injection molding-based additive manufacturing with standard equipment for soft-lithography. The method achieves 3D fabrication without specific instruments or water-soluble support material. It provides the same level of minimum membrane thickness as a high end model commercial 3D printer, and the bond strength between the membranes have a sufficient pressure resistance to the flow of water with a syringe pump. Finally, two types of 3D microstructures were fabricated by the method: the 3D fractal structure called Menger sponge, and a channel within a channel structure for 3D sheath flow.

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  63. Development of immuno-wall devices and a mobile fluorescence reader for on-site sample-to-answer immunoassay

    Kasama T., Hasegawa Y., Kondo H., Ozawa T., Kaji N., Tokeshi M., Baba Y.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014     頁: 935 - 937   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014  

    We have developed a novel immunoassay device which has a long and thin wall structure inside a microchannel, hence the name 'immuno-wall device'. Unreacted antigens and antibodies were completely removed by just immersing the device in a washing buffer for 1 minute. The long structure also allowed us to analyze fluorescence intensity by using inexpensive desktop fluorescence reader ($6,000) instead of expensive fluorescence microscopes. By using the immuno-wall devices and the fluorescence reader, the high-sensitivity C-reactive protein assays were performed with sample-in-answer-out in 15 minutes.

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  64. Ultrafast separation of small biomolecules by three-dimensional nanowire structure

    Rahong S., Yasui T., Yanagida T., Kanai M., Nagashima K., Kaji N., Kawai T., Baba Y.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014     頁: 2432 - 2434   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014  

    We report on fast biomolecules separation using three-dimensional nanowires structure devices. The three-dimensional nanowire structure can be synthesized and embedded in microchannel by Vapor-Liquid-Solid (VLS) technique. We decorate Au catalyst along the nanowires by DC sputtering and growth nanowire as a cycle to prepare the branch of the nanowire structure. This technique allows researchers to control the pore size of the three-dimensional nanowires by increasing the number of growth time. We separated small DNA molecules mixture (50-100 bp) within 35 seconds and proteins mixture (20-66 kDa) within 2.5 seconds under DC electric fields.

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  65. Sizing and sorting of single DNA molecules by microfluidic molecular combing device

    Onoshima D., Kawakita N., Takeshita D., Yukawa H., Baba Y.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014     頁: 1775 - 1777   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014  

    We report a microfluidic molecular combing device to trap, stretch, and separate DNA at a singlemolecule resolution. In order to align long DNA into a single direction on surface, we demonstrated molecular combing by dewetting DNA solution inside a microfluidic channel. It used a surface tensional force at the air-water interface of DNA solution translating on a fabricated pattern (zigzag line). When the speed of molecular translation and adsorption were balanced by pressure control, DNA was stretched along the direction of flow. By specifying the location of DNA attachment at the zigzag vertices, the device served as a molecular ruler for sizing and sorting of single DNA molecules.

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  66. Quantitative evaluation of insulating performance on silicon nanobiodevices for fully integrated nanopore single DNA sequencing

    Sano M., Yasui T., Kaji N., Taniguchi M., Kawai T., Baba Y.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014     頁: 1479 - 1481   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014  

    To realize a silicon-based semiconductor nanopore DNA sequencer, we have quantitatively evaluated insulating performance of silicon-based nanoslit array structures to stretch a random-coiled DNA molecule by electrokinetic force as a step prior to nanopore DNA sequencing. We found critical electric fields which trigger electric breakdown as a function of buffer concentration and successfully observed a single DNA molecular stretching in the nanoslit array structures under the explored conditions.

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  67. Nanobiodevices for single DNA and cell analysis

    Kaji N., Baba Y.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014     頁: 264 - 266   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014  

    Nanopore-based DNA sequencing is an emerging technology that may soon overcome even the current next-generation sequencing from the viewpoint of DNA sequencing cost and throughput. In this paper, we will show that recent advances of the "pretreatment" process using various nanostructures such as nanopillars, nanowires, and nanoslits structures inside microchannel. In addition, another type of micro and nanostructures such as micro and nanometer-scale chamber array on a chip becomes a powerful new tool for bioanalysis since it could stochastically capture and measure biomolecules at a single molecule level. The applications of these chamber array structures for single cell analysis will be also described.

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  68. Micropore channell-based simultaneous electrical and optical sensing from single biomolecules, single exosomes to single cells

    Yasaki H., Yasui T., Rahong S., Yanagida T., Kaji N., Kanai M., Nagashima K., Kawai T., Baba Y.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014     頁: 2161 - 2163   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014  

    We realized simultaneous measurements of ionic current and fluorescent imaging in microscale devices towards wide variety analysis of biomolecules, exosomes (<200 nm), circulating tumor cells (<20 μm), and white blood cells (<30 μm). The detection system based on ionic current would distinguish object sizes ranging from 500 nm to 3 μm diameter and fluorescence imaging enabled to detect target objects based on the optical properties. This electrical/optical approach might provide a new sensing scheme to analyze single biomolecules, single exosomes, and single cells with various sizes, shapes, and characteristics.

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  69. Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation

    Takeshita D., Onoshima D., Hiroshi Y., Yasui T., Kaji N., Baba Y.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014     頁: 2348 - 2350   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014  

    We combined a microfluidic stretch of a single DNA molecule and a visualization technique with fluorescent gold nanoparticle labeling, which can be observed with fluorescence microscope and scanning electron microscope (SEM) for imaging of methylated site on a single DNA molecule. It will realize bimodal microscope imaging of methylated site and enhance the imaging resolution down to nanometer level toward epigenetic analysis on the basis of high-resolution mapping of methylation sites.

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  70. Geometrical control of a single DNA molecule translocation velocity in nanopore channels

    Sun X., Yasui T., Rahong S., Yanagida T., Kaji N., Kanai M., Nagashima K., Kawai T., Baba Y.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014     頁: 1338 - 1340   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014  

    The geometrical control of translocation velocity for single DNA molecules in nanoscale space was studied in this paper. We fabricated the nanopore channels which have 250 μm in length, 300 nm in width and 300 nm in depth for confinement of single DNA molecules, and measurement of single DNA molecules translocation velocities. Single DNA molecules translocation velocities in nanopore channels were controlled by fabricating the different nanostructures in front of nanopore channels, such as shallow channels and nanopillars. Based on these strategies, we successfully decreased the single DNA molecules translocation velocities to 7.7 ± 0.8×10<sup>-3</sup> cm/s in nanopore channels.

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  71. Exosomal micro-RNA analysis in urine or serum using nanowire structures

    Yasui T., Ito S., Yanagida T., He Y., Rahong S., Kanai M., Nagashima K., Yukawa H., Kaji N., Kawai T., Baba Y.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014     頁: 680 - 682   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014  

    We report a highly-efficient separation of exosomes in 1 mL urine or serum using nanowire structures, and an extraction of exosomal micro-RNA(miRNA) from the separated exosomes. A series of procedures can be demonstrated with no any troublesome techniques, just only by introducing solution into nanowire structures embedded in microchannels. Such an easy analysis method for exosomal miRNA, especially from 1mL urine, could contribute to less invasive diagnosis and unknown biomarkers discovery.

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  72. Exosomal membrane protein detection by nanowire device

    Konakade Y., Yasui T., Yanagida T., Kaji N., He Y., Kanai M., Nagashima K., Yukawa H., Kawai T., Baba Y.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014     頁: 2017 - 2019   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014  

    We analyzed exosomal membrane proteins using ZnO nanowire devices, quantitatively and qualitatively. The nanowire devices could capture plenty of exosomes quickly, resulting in rapid analysis of exosomal membrane proteins in response to exosome concentrations, rather than conventional methods. A potential of the nanowire devices would open a window for early cancer detection.

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  73. A DNA microarray-based analysis of immune-stimulatory and transcriptional responses of dendritic cells to KALA-modified nanoparticles. 国際誌

    Akita H, Ishii S, Miura N, Shaheen SM, Hayashi Y, Nakamura T, Kaji N, Baba Y, Harashima H

    Biomaterials   34 巻 ( 35 ) 頁: 8979 - 90   2013年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Biomaterials  

    Technologies for the transfection of antigen-encoding genes into the dendritic cells, and subsequent immune-activation are both prerequisites for a successful DNA vaccine. We herein report on the density-dependent enhancement of transgene expression by the simple modification by stearyl-conjugated KALA, an α-helical peptide (STR-KALA), onto a lipid envelope-type nanoparticle (the R8-MEND, an octaarginine-modified multifunctional envelope-type nano device). The enhanced transgene expression in the KALA-modified R8-MEND (R8/KALA-MEND) cannot be explained by cellular uptake and nuclear delivery efficacy. Thus, the post-nuclear delivery process (i.e. transcription), but not intracellular trafficking processes attributed the enhanced transfection efficacy. Microarray analyses revealed that transfection with the R8/KALA-MEND resulted in a greater perturbation in host genes expression in comparison with the R8-MEND and that this effect was time-dependent. Further pathway analyses in the category of transcription-related genes and a gene ontology analysis indicated that the R8/KALA-MEND stimulated the expression of transcription factors that are closely related to immune-activation (i.e. NF- kB and STAT). Inhibition of the transfection efficacy by blockage of the STAT pathways revealed that the enhanced transcription activity is the result of immune-stimulation. Collectively, the R8/KALA-MEND mounts a "switch-on" function that triggers signal transduction forward to the immune-stimulation analogous to an adjuvant, and consequently elicits active transcription. © 2013 Elsevier Ltd.

    DOI: 10.1016/j.biomaterials.2013.08.003

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  74. Direct conversion of silver complexes to nanoscale hexagonal columns on a copper alloy for plasmonic applications.

    Yamamoto YS, Hasegawa K, Hasegawa Y, Takahashi N, Kitahama Y, Fukuoka S, Murase N, Baba Y, Ozaki Y, Itoh T

    Physical chemistry chemical physics : PCCP   15 巻 ( 35 ) 頁: 14611 - 5   2013年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Physical Chemistry Chemical Physics  

    We introduced a novel method for the rapid synthesis of silver nanohexagonal thin columns from an aqueous mixture of sodium thiosulfate (Na2S2O3) and silver chloride (AgCl) simply added to a phosphor bronze substrate. The reaction is based on galvanic displacement and the products are potentially useful for plasmonic applications. This journal is © the Owner Societies 2013.

    DOI: 10.1039/c3cp52564c

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  75. High speed DNA denaturation using microheating devices 国際誌

    Furuhashi Masayuki, Okamoto Yukihiro, Onoshima Daisuke, Ohshiro Takahito, Ryuzaki Sou, Yokota Kazumichi, Tsutsui Makusu, Taniguchi Masateru, Nakatani Kazuhiko, Baba Yoshinobu, Kawai Tomoji

    APPLIED PHYSICS LETTERS   103 巻 ( 2 )   2013年7月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Applied Physics Letters  

    Denaturation is a first step for further treatment of DNA and is expected to be carried out rapidly on an integrated chip. A microheater is a promising device for the denaturation because of easiness for fabrication and operation. In the present study, we fabricated a microheater and thermometers on a coverslip and investigated response of temperature to application of voltage. In addition, our experiment and simulation proved local heating at an aimed area. Finally, we demonstrated denaturation of DNA in buffer solution, the result of which proved that the DNA around the heater denatured within 60 ms. © 2013 AIP Publishing LLC.

    DOI: 10.1063/1.4813552

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  76. Reduced plasma glucose by asparagine synthetase knockdown in the mouse liver.

    Iida S, Kamiya H, Nakaya A, Hayashi Y, Sawada A, Kaji N, Baba Y, Harashima H

    Biological & pharmaceutical bulletin   36 巻 ( 12 ) 頁: 2009 - 11   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Biological and Pharmaceutical Bulletin  

    Expression of the asparagine synthetase gene is dependent on extracellular glucose concentration. This gene was knocked-down in livers of male Balb/c mice by a hydrodynamic tail vein injection of small interfering RNA (siRNA) against the gene. This knockdown resulted in a significant decrease in plasma glucose concentration. These results suggested that asparagine synthetase is a novel protein that regulates plasma glucose levels. © 2013 The Pharmaceutical Society of Japan.

    DOI: 10.1248/bpb.b13-00414

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  77. Is Higher Concentration of Linear Polyacrylamide Not Suitable for the Capillary Electrophoretic Separation of Large DNA?

    UEDA Masanori, BABA Yoshinobu

    Analytical Sciences   13 巻 ( 1 ) 頁: 109 - 112   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:社団法人 日本分析化学会  

    DOI: 10.2116/analsci.13.109

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  78. Fabrication of a Robust In<sub>2</sub>O<sub>3</sub> Nanolines FET Device as a Biosensor Platform. 国際誌

    Zhu Z, Yasui T, Liu Q, Nagashima K, Takahashi T, Shimada T, Yanagida T, Baba Y

    Micromachines   12 巻 ( 6 )   2021年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/mi12060642

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  79. Rapid Discrimination of Extracellular Vesicles by Shape Distribution Analysis. 国際誌

    Ryuzaki S, Yasui T, Tsutsui M, Yokota K, Komoto Y, Paisrisarn P, Kaji N, Ito D, Tamada K, Ochiya T, Taniguchi M, Baba Y, Kawai T

    Analytical chemistry   93 巻 ( 18 ) 頁: 7037 - 7044   2021年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Chemistry  

    A rapid and simple cancer detection method independent of cancer type is an important technology for cancer diagnosis. Although the expression profiles of biological molecules contained in cancer cell-derived extracellular vesicles (EVs) are considered candidates for discrimination indexes to identify any cancerous cells in the body, it takes a certain amount of time to examine these expression profiles. Here, we report the shape distributions of EVs suspended in a solution and the potential of these distributions as a discrimination index to discriminate cancer cells. Distribution analysis is achieved by low-aspect-ratio nanopore devices that enable us to rapidly analyze EV shapes individually in solution, and the present results reveal a dependence of EV shape distribution on the type of cells (cultured liver, breast, and colorectal cancer cells and cultured normal breast cells) secreting EVs. The findings in this study provide realizability and experimental basis for a simple method to discriminate several types of cancerous cells based on rapid analyses of EV shape distributions.

    DOI: 10.1021/acs.analchem.1c00258

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  80. Urinary MicroRNA-Based Diagnostic Model for Central Nervous System Tumors Using Nanowire Scaffolds. 国際誌

    Kitano Y, Aoki K, Ohka F, Yamazaki S, Motomura K, Tanahashi K, Hirano M, Naganawa T, Iida M, Shiraki Y, Nishikawa T, Shimizu H, Yamaguchi J, Maeda S, Suzuki H, Wakabayashi T, Baba Y, Yasui T, Natsume A

    ACS applied materials & interfaces   13 巻 ( 15 ) 頁: 17316 - 17329   2021年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACS Applied Materials and Interfaces  

    There are no accurate mass screening methods for early detection of central nervous system (CNS) tumors. Recently, liquid biopsy has received a lot of attention for less-invasive cancer screening. Unlike other cancers, CNS tumors require efforts to find biomarkers due to the blood-brain barrier, which restricts molecular exchange between the parenchyma and blood. Additionally, because a satisfactory way to collect urinary biomarkers is lacking, urine-based liquid biopsy has not been fully investigated despite the fact that it has some advantages compared to blood or cerebrospinal fluid-based biopsy. Here, we have developed a mass-producible and sterilizable nanowire-based device that can extract urinary microRNAs efficiently. Urinary microRNAs from patients with CNS tumors (n = 119) and noncancer individuals (n = 100) were analyzed using a microarray to yield comprehensive microRNA expression profiles. To clarify the origin of urinary microRNAs of patients with CNS tumors, glioblastoma organoids were generated. Glioblastoma organoid-derived differentially expressed microRNAs (DEMs) included 73.4% of the DEMs in urine of patients with parental tumors but included only 3.9% of those in urine of noncancer individuals, which suggested that many CNS tumor-derived microRNAs could be identified in urine directly. We constructed the diagnostic model based on the expression of the selected microRNAs and found that it was able to differentiate patients and noncancer individuals at a sensitivity and specificity of 100 and 97%, respectively, in an independent dataset. Our findings demonstrate that urinary microRNAs extracted with the nanowire device offer a well-fitted strategy for mass screening of CNS tumors.

    DOI: 10.1021/acsami.1c01754

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  81. Co-continuous structural effect of size-controlled macro-porous glass membrane on extracellular vesicle collection for the analysis of miRNA. 国際誌

    Yukawa H, Yamazaki S, Aoki K, Muto K, Kihara N, Sato K, Onoshima D, Ochiya T, Tanaka Y, Baba Y

    Scientific reports   11 巻 ( 1 ) 頁: 8672 - 8672   2021年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Scientific Reports  

    Recent studies have shown that extracellular vesicles (EVs) can be utilized as appropriate and highly specific biomarkers in liquid biopsy for the diagnosis and prognosis of serious illness. However, there are few methods that can collect and isolate miRNA in EVs simply, quickly and efficiently using general equipment such as a normal centrifuge. In this paper, we developed an advanced glass membrane column (AGC) device incorporating a size-controlled macro-porous glass (MPG) membrane with a co-continuous structure to overcome the limitations of conventional EV collection and miRNA extraction from the EVs. The size of macro-pores in the MPG membrane could be accurately controlled by changing the heating temperature and time on the basis of spinodal decomposition of B2O3, Na2O, and SiO2 in phase separation. The AGC device with an MPG membrane could collect the EVs simply and quickly (< 10 min) from cell culture supernatant, serum and urine. This AGC device could extract miRNA from the EVs captured in the MPG membrane with high efficiency when combined with a miRNA extraction solution. We suggest that the AGC device with an MPG membrane can be useful for the diagnosis and prognosis of serious illness using of EVs in various kinds of body fluids.

    DOI: 10.1038/s41598-021-87986-2

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  82. Rational Strategy for Space-Confined Seeded Growth of ZnO Nanowires in Meter-Long Microtubes. 国際誌

    Kamei R, Hosomi T, Kanao E, Kanai M, Nagashima K, Takahashi T, Zhang G, Yasui T, Terao J, Otsuka K, Baba Y, Kubo T, Yanagida T

    ACS applied materials & interfaces   13 巻 ( 14 ) 頁: 16812 - 16819   2021年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACS Applied Materials and Interfaces  

    Seeded crystal growths of nanostructures within confined spaces offer an interesting approach to design chemical reaction spaces with tailored inner surface properties. However, such crystal growth within confined spaces tends to be inherently difficult as the length increases as a result of confinement effects. Here, we demonstrate a space-confined seeded growth of ZnO nanowires within meter-long microtubes of 100 μm inner diameter with the aspect ratio of up to 10 »000, which had been unattainable to previous methods of seeded crystal growths. ZnO nanowires could be grown via seeded hydrothermal crystal growth for relatively short microtubes below the length of 40 mm, while any ZnO nanostructures were not observable at all for longer microtubes above 60 mm with the aspect ratio of 600. Microstructural and mass spectrometric analysis revealed that a conventional seed layer formation using zinc acetate is unfeasible within the confined space of long microtubes as a result of the formation of detrimental residual Zn complex compounds. To overcome this space-confined issue, a flow-assisted seed layer formation is proposed. This flow-assisted method enables growth of spatially uniform ZnO nanowires via removing residual compounds even for 1 m long microtubes with the aspect ratio of up to 10 »000. Finally, the applicably of ZnO-nanowire-decorated long microtubes for liquid-phase separations was demonstrated.

    DOI: 10.1021/acsami.0c22709

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  83. Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA. 国際誌

    Takahashi H, Yasui T, Kashida H, Makino K, Shinjo K, Liu Q, Shimada T, Rahong S, Kaji N, Asanuma H, Baba Y

    Nanotechnology   32 巻 ( 25 )   2021年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nanotechnology  

    Detection of cell-free DNA (cfDNA) has an impact on DNA analysis in liquid biopsies. However, current strategies to detect cfDNA have limitations that should be overcome, such as having low sensitivity and requiring much time and a specialized instrument. Thus, non-invasive and rapid detection tools are needed for disease prevention and early-stage treatment. Here we developed a device having a microheater integrated with zinc oxide nanowires (microheater-ZnO-NWs) to detect target single-stranded DNAs (ssDNAs) based on DNA probe hybridization. We confirmed experimentally that our device realized in-situ annealed DNA probes by which we subsequently detected target ssDNAs. We envision that this device can be utilized for fundamental studies related to nanobiodevice-based DNA detection.

    DOI: 10.1088/1361-6528/abef2c

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  84. The impact of surface Cu<sup>2+</sup>of ZnO/(Cu<inf>1−x</inf>Zn<inf>x</inf>)O heterostructured nanowires on the adsorption and chemical transformation of carbonyl compounds

    Liu J., Nagashima K., Nagamatsu Y., Hosomi T., Saito H., Wang C., Mizukami W., Zhang G., Samransuksamer B., Takahashi T., Kanai M., Yasui T., Baba Y., Yanagida T.

    Chemical Science   12 巻 ( 14 ) 頁: 5073 - 5081   2021年4月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Chemical Science  

    The surface cation composition of nanoscale metal oxides critically determines the properties of various functional chemical processes including inhomogeneous catalysts and molecular sensors. Here we employ a gradual modulation of cation composition on a ZnO/(Cu1−xZnx)O heterostructured nanowire surface to study the effect of surface cation composition (Cu/Zn) on the adsorption and chemical transformation behaviors of volatile carbonyl compounds (nonanal: biomarker). Controlling cation diffusion at the ZnO(core)/CuO(shell) nanowire interface allows us to continuously manipulate the surface Cu/Zn ratio of ZnO/(Cu1−xZnx)O heterostructured nanowires, while keeping the nanowire morphology. We found that surface exposed copper significantly suppresses the adsorption of nonanal, which is not consistent with our initial expectation since the Lewis acidity of Cu2+is strong enough and comparable to that of Zn2+. In addition, an increase of the Cu/Zn ratio on the nanowire surface suppresses the aldol condensation reaction of nonanal. Surface spectroscopic analysis and theoretical simulations reveal that the nonanal molecules adsorbed at surface Cu2+sites are not activated, and a coordination-saturated in-plane square geometry of surface Cu2+is responsible for the observed weak molecular adsorption behaviors. This inactive surface Cu2+well explains the mechanism of suppressed surface aldol condensation reactions by preventing the neighboring of activated nonanal molecules. We apply this tailored cation composition surface for electrical molecular sensing of nonanal and successfully demonstrate the improvements of durability and recovery time as a consequence of controlled surface molecular behaviors.

    DOI: 10.1039/d1sc00729g

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  85. A thermally robust and strongly oxidizing surface of WO<inf>3</inf>hydrate nanowires for electrical aldehyde sensing with long-term stability

    Zhang G., Hosomi T., Mizukami W., Liu J., Nagashima K., Takahashi T., Kanai M., Sugiyama T., Yasui T., Aoki Y., Baba Y., Ho J.C., Yanagida T.

    Journal of Materials Chemistry A   9 巻 ( 9 ) 頁: 5815 - 5824   2021年3月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Journal of Materials Chemistry A  

    Electrical molecular nanosensors require two conflicting surface characteristics, which are catalytic activity for molecular selectivity and thermal stability for long-term data collection. Here, we show that a simple surface treatment using strong acids creates two such conflicting surface properties of WO3hydrate nanowires, which enhance the electrical molecular sensing of aldehydes (nonanal, a biomarker). Mass-spectrometric measurements reveal that the surface treatment using strong acids substantially promotes both the oxidization of nonanal and the desorption of products, nonanoic acid, from the surfaces at a temperature of 50 °C, which is lower than the 300 °C required for untreated surfaces. Spectroscopic and structural measurements combined with numerical simulations identify two different adsorption structures of carbonyl groups on the surface, where molecules directly bound to coordinatively unsaturated surface tungsten preferentially proceed to the catalytic oxidization reaction and the subsequent desorption process. Furthermore, we confirm the thermal durability (over 10 years) of the catalytic activity on acid-treated WO3hydrate nanowire surfaces up to 300 °C, which enables us to demonstrate the long-term stable sensor operations with the sensitivity (4 orders of magnitude) remaining for years.

    DOI: 10.1039/d0ta11287a

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  86. Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular thermometry. 国際誌

    Nishimura Y, Oshimi K, Umehara Y, Kumon Y, Miyaji K, Yukawa H, Shikano Y, Matsubara T, Fujiwara M, Baba Y, Teki Y

    Scientific reports   11 巻 ( 1 ) 頁: 4248 - 4248   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Scientific Reports  

    Measuring optically detected magnetic resonance (ODMR) of diamond nitrogen vacancy centers significantly depends on the photon detectors used. We study camera-based wide-field ODMR measurements to examine the performance in thermometry by comparing the results to those of the confocal-based ODMR detection. We show that the temperature sensitivity of the camera-based measurements can be as high as that of the confocal detection and that possible artifacts of the ODMR shift are produced owing to the complexity of the camera-based measurements. Although measurements from wide-field ODMR of nanodiamonds in living cells can provide temperature precisions consistent with those of confocal detection, the technique requires the integration of rapid ODMR measurement protocols for better precisions. Our results can aid the development of camera-based real-time large-area spin-based thermometry of living cells.

    DOI: 10.1038/s41598-021-83285-y

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  87. Microfluidic-based capture and release of cancer-derived exosomes via peptide-nanowire hybrid interface. 国際誌

    Suwatthanarak T, Thiodorus IA, Tanaka M, Shimada T, Takeshita D, Yasui T, Baba Y, Okochi M

    Lab on a chip   21 巻 ( 3 ) 頁: 597 - 607   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Lab on a Chip  

    Cancer-derived circulating exosomes or nanoscale extracellular vesicles are emerging biomarkers for disease detection and treatment because of their cell-specific constituents and unique intercellular pathways. For efficient exosome isolation from bio-fluids, the design of high-affinity nanointerfaces is of great importance in the development of miniaturized systems for the collection of exosomes. Herein, we report peptide-functionalized nanowires as a biorecognition interface for the capture and release of cancer-derived exosomes within a microfluidic channel. Based on the amino-acid sequence of EWI-2 protein, a partial peptide that bound to the CD9 exosome marker and thus targeted cancer exosomes was screened. Linkage of the exosome-targeting peptide with a ZnO-binding sequence allowed one-step and reagent-free peptide modification of the ZnO nanowire array. As a result of peptide functionalization, the exosome-capturing ability of ZnO nanowires was significantly improved. Furthermore, the captured exosomes could be subsequently released from the nanowires under a neutral salt condition for downstream applications. This engineered surface that enhances the nanowires' efficiency in selective and controllable collection of cancer-derived exosomes provides an alternative foundation for developing microfluidic platforms for exosome-based diagnostics and therapeutics.

    DOI: 10.1039/d0lc00899k

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  88. Determination of three antiepileptic drugs in pharmaceutical formulations using microfluidic chips coupled with light-emitting diode induced fluorescence detection. 査読有り 国際誌

    Zeid AM, Nasr JJM, Belal F, Walash MI, Baba Y, Kaji N

    Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy   246 巻   頁: 119021 - 119021   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy  

    In this study, a facile, sensitive, and precise lab-on-a-chip electrophoretic method coupled with light-emitting diode induced fluorescence (LED-IF) detection was developed to assay three antiepileptic drugs, namely, vigabatrin, pregabalin, and gabapentin, in pharmaceutical formulations. The analytes were derivatised offline for the first time with fluorescine-5-isothiocyanate (FITC) to yield highly fluorescent derivatives with λex/em of 490/520 nm. The FITC-labelled analytes were injected, separated, and quantitated by a microfluidic electrophoresis device using fluorescence detection. The labelled analytes were monitored using a blue LED-IF system. The separation conditions were significantly optimised adding specific concentrations of heptakis-(2,6-di-O-methyl)-β-cyclodextrin (HDM-β-CD) and methylcellulose to the buffer solution (40 mM borate buffer). HDM-β-CD acted as a selective host for the studied antiepileptic drugs, rendering a high separation efficiency. Methylcellulose was used as an efficient dynamic coating polymer to prevent the labelled drugs from being adsorbed on the inner surfaces of the poly (methylmethacrylate) microchannels. A laboratory-prepared ternary mixture of the three antiepileptic drugs was separated within 100 s with acceptable resolution between all the three analytes (Rs > 3) and a high number of theoretical plates (N) for each analyte (N ≈ 106 plates/m). The sensitivity of the method was enhanced approximately 80-fold by stacking to yield a detection limit below 0.6 ng mL−1 in the concentration range of 2.0–200.0 ng mL−1. The method was successfully validated for analysing the studied drugs in their pharmaceutical formulations.

    DOI: 10.1016/j.saa.2020.119021

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  89. ZnO/SiO<sub>2</sub> core/shell nanowires for capturing CpG rich single-stranded DNAs. 国際誌

    Musa M, Yasui T, Nagashima K, Horiuchi M, Zhu Z, Liu Q, Shimada T, Arima A, Yanagida T, Baba Y

    Analytical methods : advancing methods and applications   13 巻 ( 3 ) 頁: 337 - 344   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Methods  

    Atomic layer deposition (ALD) is capable of providing an ultrathin layer on high-aspect ratio structures with good conformality and tunable film properties. In this research, we modified the surface of ZnO nanowires through ALD for the fabrication of a ZnO/SiO2 (core/shell) nanowire microfluidic device which we utilized for the capture of CpG-rich single-stranded DNAs (ssDNA). Structural changes of the nanowires while varying the number of ALD cycles were evaluated by statistical analysis and their relationship with the capture efficiency was investigated. We hypothesized that finding the optimum number of ALD cycles would be crucial to ensure adequate coating for successful tuning to the desired surface properties, besides promoting a sufficient trapping region with optimal spacing size for capturing the ssDNAs as the biomolecules traverse through the dispersed nanowires. Using the optimal condition, we achieved high capture efficiency of ssDNAs (86.7%) which showed good potential to be further extended for the analysis of CpG sites in cancer-related genes. This finding is beneficial to the future design of core/shell nanowires for capturing ssDNAs in biomedical applications. This journal is

    DOI: 10.1039/d0ay02138e

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  90. Oxide Nanowire Microfluidic Devices for Capturing Single-stranded DNAs.

    Musa M, Yasui T, Zhu Z, Nagashima K, Ono M, Liu Q, Takahashi H, Shimada T, Arima A, Yanagida T, Baba Y

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:社団法人 日本分析化学会  

    DOI: 10.2116/analsci.20P421

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  91. Solid-State Nanopore Platform Integrated with Machine Learning for Digital Diagnosis of Virus Infection. 国際誌

    Arima A, Tsutsui M, Washio T, Baba Y, Kawai T

    Analytical chemistry   93 巻 ( 1 ) 頁: 215 - 227   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Chemistry  

    DOI: 10.1021/acs.analchem.0c04353

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  92. Microfluidic fast chiral separation of baclofen and phenylalanine enantiomers based on cyclodextrin-electrokinetic chromatography 国際誌

    Zeid A.M., Nasr J.J.M., Belal F., Walash M., Kaji N., Baba Y.

    Microchemical Journal   160 巻   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Microchemical Journal  

    A green chiral lab-on-a-chip cyclodextrin-based microchip electrophoretic method was developed for enantioseparation of baclofen (BCN) and phenylalanine (Phe) enantiomers for the first time. All BCN and Phe enantiomers were offline labeled with 4-fluoro-7-nitrobenzofurazan (NBD-F) or fluorescein-5-isothiocyanate (FITC) fluorogenic reagents prior to microfluidic injection in a dynamically coated polymethylmethacrylate microchip. The enantioseparation was performed using borate buffer solution (50 mM; pH 9.5) containing a dynamic coating polymer (methylcellulose) and a chiral selector of cyclodextrin (CD) family as the background electrolyte. The combination of sieving properties of methylcellulose polymer and inclusion complex formation properties of the cyclodextrins rendered a novel green pseudo-stationary phase for the microfluidic chiral separation of the studied enantiomers in short separation time. Seven CD chiral selectors were investigated in our study to illustrate the proposed enantioseparation mechanism in the dynamically coated microfluidic channels. The results indicated that use of a modified cyclodextrin, heptakis-(2,6-di-O-methyl)-β-cyclodextrin (HDM-β-CD), resulted in extra interactions with the analytes of interest, leading to extra resolution with high number of theoretical plates. Therefore, HDM-β-CD was able to separate the NBD-labeled and FITC-labeled enantiomers of BCN and Phe within short analytical time. Sensitivity problem of microchip electrophoresis was overcome by application of field-enhanced injection stacking which improved sensitivity to a higher extent by online preconcentration of the labeled enantiomers in the microfluidic channels. The method was fully validated and successfully applied for the assay of BCN enantiomers and Phe enantiomers in their pure racemic mixtures. Moreover, the developed method was applied for precise analysis of BCN enantiomers in pharmaceutical formulations and in biological samples. The extraction recovery values of BCN enantiomers in biological samples were > 87.5% and %RSD < 7.0.

    DOI: 10.1016/j.microc.2020.105770

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  93. ナノデバイスを用いた細胞外小胞・エクソソームの分離精製 招待有り 査読有り

    安井隆雄、馬場嘉信

    化学工学   85 巻   2021年

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    掲載種別:研究論文(学術雑誌)  

  94. 尿中マイクロRNA測定技術 招待有り 査読有り

    安井隆雄、馬場嘉信

    ぶんせき   6 巻   頁: 204 - 207   2021年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  95. Nanobiodevices for the isolation of circulating nucleic acid for biomedical applications 招待有り 査読有り

    H. Takahashi, T. Yasui, Y. Baba

    Chem Lett.     2021年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: doi.org/10.1246/cl.210066

  96. これからの化学を考える 2100年の分析化学 招待有り 査読有り

    馬場嘉信

    現代化学創刊50周年記念号   3 巻   頁: 41 - 43   2021年

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    記述言語:日本語  

  97. Digital Pathology Platform for Respiratory Tract Infection Diagnosis via Multiplex Single-Particle Detections. 査読有り 国際誌

    Arima A, Tsutsui M, Yoshida T, Tatematsu K, Yamazaki T, Yokota K, Kuroda S, Washio T, Baba Y, Kawai T

    ACS sensors   5 巻 ( 11 ) 頁: 3398 - 3403   2020年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACS Sensors  

    The variability of bioparticles remains a key barrier to realizing the competent potential of nanoscale detection into a digital diagnosis of an extraneous object that causes an infectious disease. Here, we report label-free virus identification based on machine-learning classification. Single virus particles were detected using nanopores, and resistive-pulse waveforms were analyzed multilaterally using artificial intelligence. In the discrimination, over 99% accuracy for five different virus species was demonstrated. This advance is accessed through the classification of virus-derived ionic current signal patterns reflecting their intrinsic physical properties in a high-dimensional feature space. Moreover, consideration of viral similarity based on the accuracies indicates the contributing factors in the recognitions. The present findings offer the prospect of a novel surveillance system applicable to detection of multiple viruses including new strains.

    DOI: 10.1021/acssensors.0c01564

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  98. Highlighting Microfluidic Research to Coincide with the Virtual MicroTAS 2020 Meeting. 査読有り 国際誌

    Baba Y

    Analytical chemistry   92 巻 ( 20 ) 頁: 13613 - 13613   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acs.analchem.0c03959

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  99. Silica Nanopillar Arrays for Monitoring Diffraction-Based Label-Free Biomolecule Separation 国際誌

    Ajiri Taiga, Yasui Takao, Maeki Masatoshi, Ishida Akihiko, Tani Hirofumi, Nishii Junji, Baba Yoshinobu, Tokeshi Manabu

    ACS APPLIED NANO MATERIALS   3 巻 ( 9 ) 頁: 8810 - 8816   2020年9月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACS Applied Nano Materials  

    Recent studies on nanopillar arrays, one type of nanofluidic device, have demonstrated various tools for bioanalysis. When carrying out nanopillar array-based separation, it is indispensable to observe biomolecules, such as DNA, proteins, and extracellular vesicles, that have fluorescence labeling; however, fluorescence labeling influences the biomolecular characteristics. Here, we have proposed label-free monitoring of biomolecule separation by using diffracted light derived from the nanopillar array that was fabricated inside a microchannel by combining laser interference lithography with general photolithography techniques. Using an electrophoresis approach, we demonstrated that our diffraction-based label-free method possessed high sensor initialization ability, and the nanopillar array device successfully monitored DNA separation without labeling bias. Results obtained using our label-free monitoring of DNA separation confirmed, for the first time, that the molecular dynamics of DNA molecules in the nanopillar array were changed in the presence or absence of fluorescent labeling. The presented concept will provide a useful tool for nonbiased monitoring of label-free biomolecule analysis in nanofluidic channels.

    DOI: 10.1021/acsanm.0c01600

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  100. Ammonia-Induced Seed Layer Transformations in a Hydrothermal Growth Process of Zinc Oxide Nanowires 国際誌

    Liu Q., Yasui T., Nagashima K., Yanagida T., Hara M., Horiuchi M., Zhu Z., Takahashi H., Shimada T., Arima A., Baba Y.

    Journal of Physical Chemistry C   124 巻 ( 37 ) 頁: 20563 - 20568   2020年9月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Journal of Physical Chemistry C  

    Ammonia is a well-known additive to promote crystal growth in hydrothermal synthesis of ZnO nanowires. Although the effect of ammonia on the nanowire growth has been intensively investigated, its influence on the seed layer, which governs the initial nanowire growth, is rarely discussed. Here, we demonstrate that ammonia strongly affects the seed layer as well as the following nanowire growth. On increasing the ammonia concentration, the nanowire density first increases and then decreases, while the nanowire growth rate keeps increasing. Experimental results and thermodynamic calculations of the initial growth process reveal that the transformation of the seed layer induced by ammonia prior to nucleation critically determines the nanowire density and thus also influences the following nanowire growth. Present results highlight the critical importance of discussing the variation of seed layers in ammonia-involved hydrothermal synthesis and suggest a novel seed engineering approach for tailoring the ZnO nanowire growth.

    DOI: 10.1021/acs.jpcc.0c05490

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  101. Mechanical Rupture-Based Antibacterial and Cell-Compatible ZnO/SiO<sub>2</sub> Nanowire Structures Formed by Bottom-Up Approaches. 査読有り 国際誌

    Shimada T, Yasui T, Yonese A, Yanagida T, Kaji N, Kanai M, Nagashima K, Kawai T, Baba Y

    Micromachines   11 巻 ( 6 )   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Micromachines  

    There are growing interests in mechanical rupture-based antibacterial surfaces with nanostructures that have little toxicity to cells around the surfaces; however, current surfaces are fabricated via top-down nanotechnologies, which presents difficulties to apply for bio-surfaces with hierarchal three-dimensional structures. Herein, we developed ZnO/SiO2 nanowire structures by using bottom-up approaches and demonstrated to show mechanical rupture-based antibacterial activity and compatibility with human cells. When Escherichia coli were cultured on the surface for 24 h, over 99% of the bacteria were inactivated, while more than 80% of HeLa cells that were cultured on the surface for 24 h were still alive. This is the first demonstration of mechanical rupture-based bacterial rupture via the hydrothermally synthesized nanowire structures with antibacterial activity and cell compatibility.

    DOI: 10.3390/mi11060610

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  102. Developing spray-freeze-dried particles containing a hyaluronic acid-coated liposome-protamine-DNA complex for pulmonary inhalation.

    Fukushige K, Tagami T, Naito M, Goto E, Hirai S, Hatayama N, Yokota H, Yasui T, Baba Y, Ozeki T

    International journal of pharmaceutics   583 巻   頁: 119338   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.ijpharm.2020.119338

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  103. Observation of ethanol-induced condensation and decondensation processes at a single DNA molecular level in microfluidic devices equipped with a rapid solution exchange system. 査読有り

    Suzuki H, Fujiyoshi K, Kaji N, Tokeshi M, Baba Y

    Analytical chemistry   92 巻 ( 13 ) 頁: 9132 - 9137   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acs.analchem.0c01417

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  104. リキッドバイオプシーの最前線 ナノバイオデバイス、AIと量子技術によるリキッドバイオプシー 国際誌

    馬場 嘉信

    The Journal of Toxicological Sciences   45 巻 ( Suppl. ) 頁: S47 - S47   2020年6月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:(一社)日本毒性学会  

    <p> 閣議決定された第5期科学技術基本計画は、IoT、人工知能などのサイバー空間とセンサ、ナノテクノロジー、バイオテクノロジーなどのフィジカル空間(現実社会)を融合させる取り組みにより、人々に豊かさをもたらす「超スマート社会」を実現することを目指している。さらに、超スマート社会の実現に加えて、世界最先端の医療技術の実現による健康長寿社会の形成を目標としている。ナノバイオデバイスは、健康長寿社会を構築するための基盤技術として研究開発が進展してきた。さらに、内閣府が決定した量子技術イノベーション戦略・AI戦略等に基づいて、人工知能や量子技術と融合することにより、超スマート社会を目指した研究開発が急速に進んでいる。</p><p> 本講演では、我々が内閣府・ImPACT、AMED再生医療実現拠点ネットワークプログラム・最先端の次世代がん診断システム開発、文科省COI-STREAM等で進めているナノバイオデバイス開発を目指した研究成果として、ナノピラーによる超高速1分子DNA解析、ナノワイヤによるエクソソーム超高効率捕捉と尿中のエクソソームmiRNA超高感度解析およびがん診断、ナノポア1分子DNAシークエンシング、量子ドットと臓器透明化によるiPS細胞のintra vitalイメージング、超スマート社会を構築するAI-Powered IoNTナノセンサなど、ナノバイオデバイス、AIと量子技術の基礎研究から臨床応用・実用化に向けた研究動向について解説する。</p><p>Y. Baba, et al., 岩波新書 医の希望、2019; Nature Biotech., 22, 337 (2004); Nature Biotech., 22, 1360 (2004); Nano Lett., 12, 6145 (2012); ACS Nano, 7, 3029 (2013); Sci. Rep. (Nature Pub. Group), 4, 5252 (2014); Nano Lett., 15, 3445 (2015); Sci. Rep. (Nature Pub. Group), 6, 31642(2016); J. Am. Chem. Soc., 139, 14137 (2017); Science Advances, 3, e1701133 (2017); Sci. Rep. (Nature Pub. Group), 8, 6765 (2018); ACS Nano, 13, 2262 (2019); Nano Lett., 19, 2443 (2019); ACS Nano, 13, 8155 (2019); Nano Lett., 20, 599 (2020); EBioMed.(published by The Lancet), in press (2020).</p>

    DOI: 10.14869/toxpt.47.1.0_S25-2

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  105. Visualization of Human Induced Pluripotent Stem Cells-Derived Three-Dimensional Cartilage Tissue by Gelatin Nanospheres.

    Murata Y, Jo JI, Yukawa H, Tsumaki N, Baba Y, Tabata Y

    Tissue engineering. Part C, Methods   26 巻 ( 5 ) 頁: 244-252   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/ten.TEC.2020.0029

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  106. A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature 査読有り 国際誌

    Yukawa H., Fujiwara M., Kobayashi K., Kumon Y., Miyaji K., Nishimura Y., Oshimi K., Umehara Y., Teki Y., Iwasaki T., Hatano M., Hashimoto H., Baba Y.

    Nanoscale Advances   2 巻 ( 5 ) 頁: 1859 - 1868   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nanoscale Advances  

    Intracellular thermometry techniques play an important role in elucidating the relationship between the intracellular temperature and stem cell functions. However, there have been few reports on thermometry techniques that can detect the intracellular temperature of cells during several days of incubation. In this study, we developed a novel quantum thermometric sensing and analysis system (QTAS) using fluorescent nanodiamonds (FNDs). FNDs could label adipose tissue-derived stem cells (ASCs) at high efficiency with 24 h of incubation, and no cytotoxicity was observed in ASCs labeled with less than 500 μg mL−1of FNDs. The peak of FNDs was confirmed at approximately 2.87 GHz with the characteristic fluorescence spectra of NV centers that could be optically detected (optically detected magnetic resonance [ODMR]). The ODMR peak clearly shifted to the high-frequency side as the temperature decreased and gave a mean temperature dependence of −(77.6 ± 11.0) kHz °C−1, thus the intracellular temperature of living ASCs during several days of culturing could be precisely measured using the QTAS. Moreover, the intracellular temperature was found to influence the production of growth factors and the degree of differentiation into adipocytes and osteocytes. These data suggest that the QTAS can be used to investigate the relationship between intracellular temperature and cellular functions.

    DOI: 10.1039/d0na00146e

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  107. Monovalent sulfur oxoanions enable millimeter-long single-crystalline h-WO<sub>3</sub> nanowire synthesis. 査読有り

    Zhang G, Wang C, Mizukami W, Hosomi T, Nagashima K, Yoshida H, Nakamura K, Takahashi T, Kanai M, Yasui T, Aoki Y, Baba Y, Yanagida T

    Nanoscale   12 巻 ( 16 ) 頁: 9058-9066   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/c9nr10565d

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  108. Targeted Phototherapy for Malignant Pleural Mesothelioma: Near-Infrared Photoimmunotherapy Targeting Podoplanin. 査読有り

    Nishinaga Y, Sato K, Yasui H, Taki S, Takahashi K, Shimizu M, Endo R, Koike C, Kuramoto N, Nakamura S, Fukui T, Yukawa H, Baba Y, K Kaneko M, Chen-Yoshikawa TF, Kobayashi H, Kato Y, Hasegawa Y

    Cells   9 巻 ( 4 )   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/cells9041019

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  109. Photolithographically Constructed Single ZnO Nanowire Device and Its Ultraviolet Photoresponse.

    Liu Q, Yasui T, Nagashima K, Yanagida T, Horiuchi M, Zhu Z, Takahashi H, Shimada T, Arima A, Baba Y

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2116/analsci.20N002

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  110. Tailored Photoluminescence Properties of Ag(In,Ga)Se<inf>2</inf> Quantum Dots for Near-Infrared in Vivo Imaging 査読有り 国際誌

    Kameyama T., Yamauchi H., Yamamoto T., Mizumaki T., Yukawa H., Yamamoto M., Ikeda S., Uematsu T., Baba Y., Kuwabata S., Torimoto T.

    ACS Applied Nano Materials   3 巻 ( 4 ) 頁: 3275 - 3287   2020年4月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACS Applied Nano Materials  

    Multinary semiconductor quantum dots (QDs) that have less toxicity and show near-infrared light responsivity have attracted much attention for in vivo bioimaging. In this study, we controlled the optical properties of Ag-In-Se QDs by modulating the nonstoichiometry and the degree of Ga3+ doping. Precise tuning of the Ag/In ratio of Ag-In-Se QDs enabled a sharp band-edge emission to emerge without broad defect-site emission. Ga3+ doping into Ag-In-Se (AIGSe) QDs enlarged their energy gap, resulting in a blue shift of the band-edge PL peak from from 890 to 630 nm. The band-edge PL intensity was remarkably enlarged by surface coating with a thin GaSx shell followed by treatment with trioctylphosphine, the highest PL yield being 38% for the PL peak at 800 nm. Thus-obtained QDs were successfully used as near-IR PL probes for three-dimensional in vivo bioimaging in which the wavelengths of excitation and detection lights could be selected in the first biological window, and then the signals were clearly detected from AIGSe@GaSx core-shell QDs injected into biological tissues by ca. 5 mm in depth.

    DOI: 10.1021/acsanm.9b02608

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  111. A Method to Analyze Urinary Extracellular Vesicles. 査読有り

    Paisrisarn P, Yasui T, Baba Y

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   36 巻 ( 7 ) 頁: 791 - 798   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2116/analsci.20R001

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  112. Mechanical Low-Pass Filtering of Cells for Detection of Circulating Tumor Cells in Whole Blood 査読有り

    Suzuki Taiki, Kaji Noritada, Yasaki Hirotoshi, Yasui Takao, Baba Yoshinobu

    ANALYTICAL CHEMISTRY   92 巻 ( 3 ) 頁: 2483 - 2491   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acs.analchem.9b03939

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  113. Near infrared photoimmunotherapy targeting DLL3 for small cell lung cancer. 査読有り 国際誌

    Isobe Y, Sato K, Nishinaga Y, Takahashi K, Taki S, Yasui H, Shimizu M, Endo R, Koike C, Kuramoto N, Yukawa H, Nakamura S, Fukui T, Kawaguchi K, Chen-Yoshikawa TF, Baba Y, Hasegawa Y

    EBioMedicine   52 巻   頁: 102632 - 102632   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:EBioMedicine  

    Background: Small cell lung cancer (SCLC) has a poor prognosis, and its treatment options are limited. Delta-like protein 3 (DLL3) is expressed specifically in SCLC and is considered a promising therapeutic target for patients with this disease. Rovalpituzumab tesirine (Rova-T) was the first antibody-drug conjugate targeting DLL3. Although Rova-T development was unfortunately terminated, DLL3 remains an ideal target for SCLC. Near infrared photoimmunotherapy (NIR-PIT) is a new form of cancer treatment that employs an antibody-photosensitiser conjugate followed by NIR light exposure and damage target cells specifically. In this study, we demonstrate DLL3-targeted NIR-PIT to develop a novel molecularly targeted treatment for SCLC. Methods: The anti-DLL3 monoclonal antibody rovalpituzumab was conjugated to an IR700 photosensitiser (termed ‘rova-IR700’). SCLC cells overexpressing DLL3 as well as non-DLL3-expressing controls were incubated with rova-IR700 and then exposed to NIR-light. Next, mice with SCLC xenografts were injected with rova-IR700 and irradiated with NIR-light. Findings: DLL3-overexpressing cells underwent immediate destruction upon NIR-light exposure, whereas the control cells remained intact. The xenograft in mice treated with rova-IR700 and NIR-light shrank markedly, whereas neither rova-IR700 injection nor NIR-light irradiation alone affected tumour size. Interpretation: Our data suggest that targeting of DLL3 using NIR-PIT could be a novel and promising treatment for SCLC. Funding: Research supported by grants from the Program for Developing Next-generation Researchers (Japan Science and Technology Agency), KAKEN (18K15923, JSPS), Medical Research Encouragement Prize of The Japan Medical Association, The Nitto Foundation, Kanae Foundation for the Promotion of Medical Science.

    DOI: 10.1016/j.ebiom.2020.102632

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  114. A Novel Treatment with Stem Cells from Human Exfoliated Deciduous Teeth for Hypoxic-Ischemic Encephalopathy in Neonatal Rats 査読有り

    Kitase Yuma, Sato Yoshiaki, Ueda Kazuto, Suzuki Toshihiko, Mikrogeorgiou Alkisti, Sugiyama Yuichiro, Matsubara Kohki, Okabe Yuka Tsukagoshi, Shimizu Shinobu, Hirata Hitoshi, Yukawa Hiroshi, Baba Yoshinobu, Tsuji Masahiro, Takahashi Yoshiyuki, Yamamoto Akihito, Hayakawa Masahiro

    STEM CELLS AND DEVELOPMENT   29 巻 ( 2 ) 頁: 63 - 74   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/scd.2019.0221

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  115. Synthesis of Monodispersedly Sized ZnO Nanowires from Randomly Sized Seeds 査読有り

    Zhao Xixi, Nagashima Kazuki, Zhang Guozhu, Hosomi Takuro, Yoshida Hideto, Akihiro Yuya, Kanai Masaki, Mizukami Wataru, Zhu Zetao, Takahashi Tsunaki, Suzuki Masaru, Samransuksamer Benjarong, Meng Gang, Yasui Takao, Aoki Yuriko, Baba Yoshinobu, Yanagida Takeshi

    NANO LETTERS   20 巻 ( 1 ) 頁: 599 - 605   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acs.nanolett.9b04367

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  116. 蛍光ナノダイヤモンドによる幹細胞再生機能温度センシング

    宮地 冬, 小林 香央里, 西村 勇姿, 藤原 正澄, 湯川 博, 馬場 嘉信

      27 巻 ( 2 ) 頁: 185 - 190   2020年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    <p>It has become clear that temperature plays a major role in many life phenomena. However, little is known about the intracellular temperature, and the relationship between regenerative function and intracellular temperature of stem cells. In this study, fluorescent nanodiamond (FND) that can be applied as an excellent quantum sensor was used for stem cell labeling. Then, the relationship between the regenerative function of stem cells and the intracellular temperature of stem cells was evaluated by a sensing the optically detected magnetic resonance (ODMR) activity of NV centers in FNDs. FNDs could label adipose tissue-derived stem cells (ASCs) at high efficiency with 24 h incubation, and no cytotoxicity was observed in ASCs labeled with less than 500 µg/mL of FNDs. The ODMR of NV centers in FNDs was confirmed at approximately 2.87 GHz with a characteristic fluorescence spectrum, and the intracellular temperature of living ASCs during several days of culturing could be precisely measured. Moreover, the intracellular temperature was found to influence the production of growth factors and the degree of differentiation into adipocytes and osteocytes. These data suggest that NV centers in FNDs can be used to investigate the relationship between intracellular temperature and cellular functions.</p>

    DOI: 10.11378/organbio.27.185

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  117. Analysis of ion components derived from particulate matter using ion selective electrodes 査読有り

    Haruka Yamauchi, Taisuke Shimada, Takao Yasui, Tatsuro Goda, Noritada Kaji, Yuji Miyahara, Yoshinobu Baba

    MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 490 - 491   2020年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    © 2020 CBMS-0001 Lifelong exposure history (exposome) of particulate matters (PMs) is essential for understanding environmentally induced health risk, however, diversities in PM size, shape and components are a technical barrier to track the exposome. Previously, we reported a PM sensor utilizing water film-based collection and microfluidics-employed ionic current measurement at MicroTAS 2018 [1], which was only applied to analyze non-soluble PMs. Herein, we realized a potentiometry-based methodology for sensing multiple ion components derived from water-soluble PMs via arrayed ion-selective electrodes (ISEs). NO3-, NH3+, and Na+ ions extracted from a real PM sample were simultaneously detected and analyzing their contents was achieved.

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  118. 九州大学伊都キャンパスにおけるPM2.5の生物学的観点からの分析と実態調査 査読有り

    三浦夏琳、井手幸子、内藤豊裕、嶋田泰佑、安井隆雄、馬場嘉信、加地範匡

    分析化学   69 巻   頁: 741 - 746   2020年

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    記述言語:日本語  

  119. Development of an immuno-wall device for the rapid and sensitive detection of EGFR mutations in tumor tissues resected from lung cancer patients. 国際誌

    Yogo N, Hase T, Kasama T, Nishiyama K, Ozawa N, Hatta T, Shibata H, Sato M, Komeda K, Kawabe N, Matsuoka K, Chen-Yoshikawa TF, Kaji N, Tokeshi M, Baba Y, Hasegawa Y

    PloS one   15 巻 ( 11 ) 頁: e0241422   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PLoS ONE  

    Detecting molecular targets in specimens from patients with lung cancer is essential for targeted therapy. Recently, we developed a highly sensitive, rapid-detection device (an immuno-wall device) that utilizes photoreactive polyvinyl alcohol immobilized with antibodies against a target protein via a streptavidin–biotin interaction. To evaluate its performance, we assayed epidermal growth factor receptor (EGFR) mutations, such as E746_A750 deletion in exon 19 or L858R substitution in exon 21, both of which are common in non-small cell lung cancer and important predictors of the treatment efficacy of EGFR tyrosine kinase inhibitors. The results showed that in 20-min assays, the devices detected as few as 1% (E746_A750 deletion) and 0.1% (L858R substitution) of mutant cells. Subsequent evaluation of detection of the mutations in surgically resected lung cancer specimens from patients with or without EGFR mutations and previously diagnosed using commercially available, clinically approved genotyping assays revealed diagnostic sensitivities of the immuno-wall device for E746_A750 deletion and L858R substitution of 85.7% and 87.5%, respectively, with specificities of 100% for both mutations. These results suggest that the immuno-wall device represents a good candidate next-generation diagnostic tool, especially for screening of EGFR mutations.

    DOI: 10.1371/journal.pone.0241422

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  120. Face-selective tungstate ions drive zinc oxide nanowire growth direction and dopant incorporation 招待有り 査読有り

    Jiangyang Liu, Kazuki Nagashima, Hiroki Yamashita, Wataru Mizukami, Jun Uzuhashi, Takuro Hosomi, Masaki Kanai, Xixi Zhao, Yoshinori Miura, Guozhu Zhang, Tsunaki Takahashi, Masaru Suzuki, Daiki Sakai, Benjarong Samransuksamer, Yong He, Tadakatsu Ohkubo, Takao Yasui, Yuriko Aoki, Johnny Ho, Yoshinobu Baba, and Takeshi Yanagida

    Communications Material     頁: 58(2020)   2020年

  121. Analysis of particulate matters via surfactant-assisted ionic current sensing 査読有り

    Keiko Fujino, Taisuke Shimada, Takao Yasui, Kazuki Nagashima, Takashi Yanagida, Noritada Kaji, Yoshinobu Baba

    MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 498 - 499   2020年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    © 2020 CBMS-0001 Toward tracking lifelong exposure history of particulate matters (PMs) for understanding its disease risk, we realized a surfactant-assisted ionic current sensing for analyzing the size and concentration of hydrophobic PMs. Previously, we reported a PM sensor via water film-based collection and micropore-employed ionic current sensing at MicroTAS 2018 [1]. However, an analysis on PM number concentration has not achieved due to a difficulty in sensing hydrophobic PMs. Here, the hydrophobic PMs were detected with the aid of sodium dodecyl sulfate (SDS) and information on PM size and number concentration was obtained.

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  122. 量子生命科学の将来展望 招待有り 査読有り

    馬場嘉信、白川昌宏、須原哲也

    Optronics   39 巻 ( 464 ) 頁: 72 - 77   2020年

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    記述言語:日本語  

  123. Metal oxide nanowires microfluidic devices as potential tool for DNA methylation mapping 査読有り 国際誌

    MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences     頁: 1093 - 1094   2020年

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    掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

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  124. The Virtual Issue on Advances in Microfluidics Research 招待有り 査読有り

    Yoshinobu Baba(Editor)

    Anal. Chem.     2020年

  125. Microfluidic Mechanotyping of a Single Cell with Two Consecutive Constrictions of Different Sizes and an Electrical Detection System

    Sano Mamiko, Kaji Noritada, Rowat Amy C., Yasaki Hirotoshi, Shao Long, Odaka Hidefumi, Yasui Takao, Higashiyama Tetsuya, Baba Yoshinobu

    ANALYTICAL CHEMISTRY   91 巻 ( 20 ) 頁: 12890-12899   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acs.analchem.9b02818

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  126. Rapid, Sensitive, and Selective Detection of H5 Hemagglutinin from Avian Influenza Virus Using an Immunowall Device 査読有り

    Chavez Ramos Kenia, Nishiyama Keine, Maeki Masatoshi, Ishida Akihiko, Tani Hirofumi, Kasama Toshihiro, Baba Yoshinobu, Tokeshi Manabu

    ACS OMEGA   4 巻 ( 15 ) 頁: 16683 - 16688   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acsomega.9b02788

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  127. Substantial Narrowing on the Width of "Concentration Window" of Hydrothermal ZnO Nanowires via Ammonia Addition 査読有り

    Sakai Daiki, Nagashima Kazuki, Yoshida Hideto, Kanai Masaki, He Yong, Zhang Guozhu, Zhao Xixi, Takahashi Tsunaki, Yasui Takao, Hosomi Takuro, Uchida Yuki, Takeda Seiji, Baba Yoshinobu, Yanagida Takeshi

    SCIENTIFIC REPORTS   9 巻 ( 1 ) 頁: 1 - 10   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-019-50641-y

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  128. Exosomal Analysis of ALK Rearrangements by Spin Column with Porous Glass Filter 国際誌

    Hatta T, Hase T, Ozawa N, Yogo N, Yukawa H, Tanaka H, Onoshima D, Sato M, Hori M, Baba Y, Hasegawa Y

    JOURNAL OF THORACIC ONCOLOGY   14 巻 ( 10 ) 頁: S676-S676   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  129. In Vivo Fluorescence Visualization of Anterior Chamber Injected Human Corneal Endothelial Cells Labeled With Quantum Dots 査読有り

    Toda Munetoyo, Yukawa Hiroshi, Yamada Jun, Ueno Morio, Kinoshita Shigeru, Baba Yoshinobu, Hamuro Junji

    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE   60 巻 ( 12 ) 頁: 4008 - 4020   2019年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1167/iovs.19-27788

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  130. Ultrasensitive detection of disease biomarkers using an immuno-wall device with enzymatic amplification 査読有り

    Nishiyama Keine, Kasama Toshihiro, Nakamata Seiya, Ishikawa Koya, Onoshima Daisuke, Yukawa Hiroshi, Maeki Masatoshi, Ishida Akihiko, Tani Hirofumi, Baba Yoshinobu, Tokeshi Manabu

    ANALYST   144 巻 ( 15 ) 頁: 4589 - 4595   2019年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/c9an00480g

    Web of Science

  131. Geometry-Based Self-Assembly of Histone-DNA Nanostructures at Single-Nucleotide Resolution 査読有り

    Serag Maged F., Aikeremu Aimaiti, Tsukamoto Ryoko, Piwonski Hubert, Abadi Maram, Kaji Noritada, Dwyer Jason R., Baba Yoshinobu, Habuchi Satoshi

    ACS NANO   13 巻 ( 7 ) 頁: 8155 - 8168   2019年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acsnano.9b03259

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  132. Micro- and Nanopillar Chips for Continuous Separation of Extracellular Vesicles 査読有り

    Hattori Yuya, Shimada Taisuke, Yasui Takao, Kaji Noritada, Baba Yoshinobu

    ANALYTICAL CHEMISTRY   91 巻 ( 10 ) 頁: 6514 - 6521   2019年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acs.analchem.8b05538

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  133. Water-Organic Cosolvent Effect on Nucleation of Solution-Synthesized ZnO Nanowires 査読有り

    Akihiro Yuya, Nagashima Kazuki, Hosomi Takuro, Kanai Masaki, Anzai Hiroshi, Takahashi Tsunaki, Zhang Guozhu, Yasui Takao, Baba Yoshinobu, Yanagida Takeshi

    ACS OMEGA   4 巻 ( 5 ) 頁: 8299 - 8304   2019年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acsomega.9b00945

    Web of Science

  134. Rational Method of Monitoring Molecular Transformations on Metal-Oxide Nanowire Surfaces 査読有り

    Wang Chen, Hosomi Takuro, Nagashima Kazuki, Takahashi Tsunaki, Zhang Guozhu, Kanai Masaki, Zeng Hao, Mizukami Wataru, Shioya Nobutaka, Shimoaka Takafumi, Tamaoka Takehiro, Yoshida Hideto, Takeda Seiji, Yasui Takao, Baba Yoshinobu, Aoki Yuriko, Terao Jun, Hasegawa Takeshi, Yanagida Takeshi

    NANO LETTERS   19 巻 ( 4 ) 頁: 2443 - 2449   2019年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acs.nanolett.8b05180

    Web of Science

  135. Collection and Sensing of PM2.5 in Microfluidic Devices 査読有り 国際誌

    Shimada T., Yasaki H., Yasui T., Kaji N., Baba Y.

    IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED   2018-December 巻   頁: 240 - 243   2019年2月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED  

    Toward evaluating health risk of particulate matter with size less than or equal to 2.5 μm (PM2.5), we propose a methodology for realizing the PM2.5 analysis in liquid phase via water film-based collection and microfluidics-based electrical detection. We reported last year that a hydrophilic nanowire surface was utilized in the collection for formation of a water film, which enabled continuous collection of air-floating PM2.5 into liquid phase and followed-by detection. As advanced results, the collected PM2.5 was successfully detected and analyzed via ion current sensing using a micropore. We believe proposed PM2.5 analysis methodology in liquid phase would allow to realize health risk evaluation and reduce health damage.

    DOI: 10.1109/NANOMED.2018.8641523

    Scopus

  136. Engineering Nanowire-Mediated Cell Lysis for Microbial Cell Identification 査読有り

    Yasui Takao, Yanagida Takeshi, Shimada Taisuke, Otsuka Kohei, Takeuchi Masaki, Nagashima Kazuki, Rahong Sakon, Naito Toyohiro, Takeshita Daiki, Yonese Akihiro, Magofuku Ryo, Zhu Zetao, Kaji Noritada, Kanai Masaki, Kawai Tomoji, Baba Yoshinobu

    ACS NANO   13 巻 ( 2 ) 頁: 2262 - 2273   2019年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acsnano.8b08959

    Web of Science

  137. Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay 査読有り

    Nishiyama Keine, Sugiura Kanako, Kaji Noritada, Tokeshi Manabu, Baba Yoshinobu

    LAB ON A CHIP   19 巻 ( 2 ) 頁: 233 - 240   2019年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/c8lc01105b

    Web of Science

  138. Monitoring spin coherence of single nitrogen-vacancy centers in nanodiamonds during pH changes in aqueous buffer solutions 査読有り

    Fujiwara Masazumi, Tsukahara Ryuta, Sera Yoshihiko, Yukawa Hiroshi, Baba Yoshinobu, Shikata Shinichi, Hashimoto Hideki

    RSC ADVANCES   9 巻 ( 22 ) 頁: 12606 - 12614   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/c9ra02282a

    Web of Science

  139. Nanobiodevices for Cancer Diagnostics and Stem Cell Therapeutics 国際誌

    Onoshima Daisuke, Yukawa Hiroshi, Baba Yoshinobu

    APPLICATIONS OF MICROFLUIDIC SYSTEMS IN BIOLOGY AND MEDICINE   7 巻   頁: 275-300 - 300   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/978-981-13-6229-3_10

    Web of Science

  140. 量子ドットによる完全透明化組織内移植幹細胞イメージング

    水巻 登志樹, 湯川 博, 洲﨑 悦生, 上田 泰己, 馬場 嘉信

    Organ Biology   26 巻 ( 2 ) 頁: 199 - 204   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    <p><i>In vivo</i> imaging technology can detect and diagnose a total of transplanted stem cells remains unestablished. In order to enhance the safety and treatment effect of regenerative medicine, it is necessary to understand where transplanted stem cells accumulate in the tissue and how accumulated cells behave in the tissue at a single cell level. In this study, we challenged the innovative diagnosis of transplanted stem cells in acute liver failure model mice by quantum dots (QDs) fluorescence imaging and whole tissue clearing technologies.</p><p>After transplantation of stem cells labeled with QDs655 into the living body of the acute liver failure model mice through tail vein, it was confirmed that the transplanted stem cells were mainly accumulated in the lung and liver of the mice using <i>in vivo</i> imaging system. In addition, we succeeded in revealing the spatiotemporal distribution of transplanted stem cells at the single cell level by organ clearing and 3D imaging of organs using light sheet fluorescence microscopy.</p>

    DOI: 10.11378/organbio.26.199

    CiNii Article

  141. 量子ドットによる幹細胞由来エクソソームイメージングと再生医療への応用

    公文 優花, 湯川 博, 小野島 大介, 馬場 嘉信

    生体医工学   57 巻 ( 0 ) 頁: S81_1-S81_1   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    <p>幹細胞による再生治療メカニズム解明は、再生医療の更なる安全性、治療効果向上において極めて重要である。幹細胞は自己複製能と多分化能を持つ特殊な細胞であり、障害部位への特異的な集積能力や再生因子産性能を持つことから患者に移植することで治療効果を発揮することが知られている。更に、近年では幹細胞から放出されるエクソソームも同様に治療効果を持つことがわかっており、新規治療ツールとして注目され始めている。しかし、エクソソームの詳細な生体内挙動及び細胞間移動等について解析できる技術が乏しく、再生機能の役割については未だ未解明のままである。本研究では、エクソソームを生体内で高感度に検出できる技術の確立を目指した。最先端量子ナノ材料である量子ドットをグルコース修飾することで、もともと細胞が持つグルコーストランスポーターを通して細胞内に導入することに成功した。また、グルコーストランスポーターを持つとされるエクソソームにおいても同様に内部への導入が確認された。エクソソームの内部に導入することで、膜上に蛍光色素を付着させる従来法に比べてより安定で高感度のラベル化に成功した。</p>

    DOI: 10.11239/jsmbe.Annual57.S81_1

    CiNii Article

  142. 量子ドットを用いた透明化組織内移植幹細胞イメージング技術の開発

    水巻 登志樹, 湯川 博, 小野島 大介, 洲崎 悦生, 上田 泰己, 馬場 嘉信

    生体医工学   57 巻 ( 0 ) 頁: S177_1-S177_1   2019年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    <p>幹細胞移植は幹細胞をそのまま、あるいは前駆細胞に分化させた状態で血流に乗せて移植する治療法であり、幹細胞を用いた組織や臓器の再構築が未だ困難とされている疾病に対して有効な治療法であるとして近年注目を集めている。幹細胞移植の実現に向けては、安全性が十分に確保されており、尚且つ治療効果が大きい移植プロトコールを確立する必要があり、この課題の解決に向けて、移植幹細胞の体内動態や生着部位を高感度にイメージングし、どのように治療効果を発揮しているかを解明する必要がある。しかし、これまで生体内のすべての移植幹細胞を網羅的にイメージング診断できる技術は確立されていない。臓器や組織は光を吸収・散乱する様々な物質から構成されており、光の透過性が極めて低いため、二光子励起顕微鏡などを用いても表面上のしかも限られた視野での観察に留まっているのが現状である。そこで我々は、臓器の効率的な透明化が可能な技術に注目し量子ドットを用いた幹細胞蛍光イメージング技術と融合することで、急性肝不全モデルマウスにおける移植幹細胞の網羅的、且つ単一細胞レベルの解像度でのイメージングを可能にする革新的診断技術の確立に取り組んだ。</p>

    DOI: 10.11239/jsmbe.Annual57.S177_1

    CiNii Article

  143. Discriminating drug-resistant bacteria using AI analysis on fine current changes from inner ION leakages 査読有り 国際誌

    Yoshikawa A.

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019     頁: 852 - 853   2019年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019  

    Scopus

  144. Analyzing peptide adsorption states via nanowire-employed infrared spectrometry 査読有り 国際誌

    Naito H.

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019     頁: 1206 - 1207   2019年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019  

    Scopus

  145. Analyzing peptide adsorption states via nanowire-employed infrared spectrometry

    Hiroki Naito, Takao Yasui, Taisuke Shimada, Nobutaka Shioya, Takafumi Shimoaka, Masayoshi Tanaka, Kazuki Nagasima, Mina Okochi, Takeshi Yanagida, Takeshi Hasegawa, Yoshinobu Baba

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019     頁: 1206 - 1207   2019年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    © 2019 CBMS-0001. Peptides optimally binding to solid surfaces are a promising candidate for adding bio-affinity, however, their binding states are not fully revealed. Herein, we realized a methodology for analyzing peptide adsorption states via employing nanowires and infrared (IR) p-polarized multiple-angle incidence resolution spectrometry (pMAIRS). Our methodology enabled to provide large crystalline surface for peptide adsorption, and enhance signal intensities. We found that a modelled peptide had different adsorption states among crystal axes. Analyzing peptide adsorption states can pave the way for controlling adsorbed states, and moreover, using full potentials of peptide.

    Scopus

  146. Hybridization-based DNA analysis by self-heating nanowire microfluidic devices 査読有り 国際誌

    Takahashi H.

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019     頁: 720 - 721   2019年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019  

    Scopus

  147. Single-cell microscopic raman spectroscopy for rapid microbial detection 査読有り 国際誌

    Onoshima D.

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019     頁: 1374 - 1375   2019年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019  

    Scopus

  148. 量子ナノ材料による移植幹細胞in vivo蛍光イメージングと再生医療 招待有り 査読有り

    湯川博、馬場嘉信

    化学工業   70 巻   頁: 234 - 240   2019年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  149. Salivary microrna corection and analysis using nanocellulose for domiciliary cancer diagnosis

    Naoya Mizukami, Takao Yasui, Hironao Koga, Yoshinobu Baba

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019     頁: 894 - 895   2019年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    © 2019 CBMS-0001. Analyzing microRNAs in our body allowed us to investigate our various vital in Here [1], our aim is that discovering additional vital information based on microRNAs from saliva samples. MicroRNAs in body fluids are known to obtain higher stability in body fluids via encapsulation in extracellular vesicles with over 40-nm diameter [2]. To capture the vesicles, we conceived an idea to use nanocellulose with several 10- to several 100-nm pore sizes. The nanocellulose made it possible to extract over 1000 species of microRNAs from 10 µL saliva within 20 s, compared to ultracentrifugation, which required over 180 min and 10 µL saliva to extract around 600 species of microRNAs.

    Scopus

  150. 分子夾雑生命化学に基づく新規計測技術のがん診断への展開 ナノバイオ夾雑環境デバイスによる肝がん細胞由来エクソソームの血管新生評価 招待有り 査読有り

    湯川博、馬場嘉信

    化学と工業   72 巻   頁: 410 - 412   2019年

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    記述言語:日本語  

  151. ナノバイオデバイスとAIが拓くSociety5.0・健康長寿社会 招待有り 査読有り

    馬場嘉信

    化学とマイクロ・ナノシステム   18 巻   頁: 1 - 6   2019年

  152. 分子夾雑と診断 がん医療への展開 招待有り 査読有り

    小野島大介、馬場嘉信

    現代化学   576 巻   頁: 37 - 39   2019年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  153. Microfluidic Mechanotyping of a Single Cell with Two ConsecutiveConstrictions of Different Sizes and an Electrical Detection System. 招待有り 査読有り

    Mamiko Sano, Noritada Kaji, Amy Rowat, Hirotoshi Yasaki, Long Shao, Hidefumi Okada, Takao Yasui, Tetsuya Higashiyama , Yoshinobu Baba

    Anal. Chem.   91 巻   頁: 12890 - 12899   2019年

  154. 酸化物ナノワイヤによる尿中microRNAの回収と解析 招待有り 査読有り

    長縄豪、安井隆雄、柳田剛、加地範匡、長島一樹、鷲尾隆、馬場嘉信

    化学とマイクロ・ナノシステム   18 巻   頁: 34 - 35   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  155. Rational Method to Monitor Molecular Transformations on Metal Oxide Nanowire Surfaces 招待有り 査読有り

    Wang C., Hosomi T., Nagashima K., Takahashi T., Zhang G., Kanai M., Zeng H., Mizukami W., Shioya N., Shimoaka T., Tamaoka T., Yoshida H., Takeda S., Yasui T., Baba Y., Aoki Y., Terao J., Hasegawa T., Yanagida T.

    Nano Lett.   19 巻 ( 4 ) 頁: 2443 - 2449   2019年

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    記述言語:英語  

  156. Identifying multiple viral species at a single particle level using a combination of nanopores and machine leaning approach 査読有り 国際誌

    Arima A.

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019     頁: 1238 - 1239   2019年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019  

    Scopus

  157. PM2.5 Particle Detection in a Microfluidic Device by Using Ionic Current Sensing

    Shimada Taisuke, Yasaki Hirotoshi, Yasui Takao, Yanagida Takeshi, Kaji Noritada, Kanai Masaki, Nagashima Kazuki, Kawai Tomoji, Baba Yoshinobu

    ANALYTICAL SCIENCES   34 巻 ( 12 ) 頁: 1347-1349   2018年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  158. Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer 査読有り

    Shimada Taisuke, Yasui Takao, Yokoyama Asami, Goda Tatsuro, Hara Mitsuo, Yanagida Takeshi, Kaji Noritada, Kanai Masaki, Nagashima Kazuki, Miyahara Yuji, Kawai Tomoji, Baba Yoshinobu

    LAB ON A CHIP   18 巻 ( 21 ) 頁: 3225 - 3229   2018年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/c8lc00438b

    Web of Science

  159. Development of the immuno-wall device for rapid detection of ALK and ROS1 fusions in lung cancer

    Yogo N., Hase T., Kasama T., Hatta T., Ozawa N., Sato M., Kaji N., Tokeshi M., Baba Y., Hasegawa Y.

    ANNALS OF ONCOLOGY   29 巻   頁: .   2018年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  160. Development of the immuno-wall device for rapid detection of ALK and ROS1 fusions in lung cancer 国際誌

    Yogo N, Hase T, Kasama T, Hatta T, Ozawa N, Sato M, Kaji N, Tokeshi M, Baba Y, Hasegawa Y

    ANNALS OF ONCOLOGY   29 巻   頁: .   2018年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/annonc/mdy441.027

    Scopus

  161. Effective Transplantation of 2D and 3D Cultured Hepatocyte Spheroids Confirmed by Quantum Dot Imaging 査読有り

    Okumura Hiroki, Nanizawa Eri, Nakanishi Anna, Yukawa Hiroshi, Hashita Tadahiro, Iwao Takahiro, Baba Yoshinobu, Ishikawa Tetsuya, Matsunaga Tamihide

    ADVANCED BIOSYSTEMS   2 巻 ( 8 )   2018年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/adbi.201800137

    Web of Science

  162. Adipose-derived mesenchymal stem cells attenuate rejection in a rat lung transplantation model 査読有り

    Watanabe Hironosuke, Tsuchiya Tomoshi, Shimoyama Koichiro, Shimizu Akira, Akita Sadanori, Yukawa Hiroshi, Baba Yoshinobu, Nagayasu Takeshi

    JOURNAL OF SURGICAL RESEARCH   227 巻   頁: 17-27   2018年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.jss.2018.01.016

    Web of Science

  163. Determination of baclofen and vigabatrin by microchip electrophoresis with fluorescence detection: application of field-enhanced sample stacking and dynamic pH junction 査読有り

    Zeid Abdallah M., Kaji Noritada, Nasr Jenny Jeehan M., Belal Fathalla, Walash Mohamed I., Baba Yoshinobu

    NEW JOURNAL OF CHEMISTRY   42 巻 ( 12 ) 頁: 9965-9974   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/c8nj00829a

    Web of Science

  164. Effects of cold exposure on metabolites in brown adipose tissue of rats 査読有り

    Hiroshima Yuka, Yamamoto Takenori, Watanabe Masahiro, Baba Yoshinobu, Shinohara Yasuo

    MOLECULAR GENETICS AND METABOLISM REPORTS   15 巻   頁: 36-42   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.ymgmr.2018.01.005

    Web of Science

  165. Low-autofluorescence fluoropolymer membrane filters for cell filtration 査読有り 国際誌

    Kihara Naoto, Kuboyama Daiki, Onoshima Daisuke, Ishikawa Kenji, Tanaka Hiromasa, Ozawa Naoya, Hase Tetsunari, Koguchi Ryohei, Yukawa Hiroshi, Odaka Hidefumi, Hasegawa Yoshinori, Baba Yoshinobu, Hori Masaru

    JAPANESE JOURNAL OF APPLIED PHYSICS   57 巻 ( 6 )   2018年6月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:Japanese Journal of Applied Physics  

    A fluoropolymer membrane filter with through-holes was fabricated by photolithographic patterning and the dry etching method. 380,000 highly packed through-holes, each with a diameter of 7 µm were able to cover a whole area with a diameter of 13 mm. Ethylene tetrafluoroethylene (ETFE) was used as the membrane, which was suitable for the fluorescence detection of rare cells such as circulating tumor cells (CTCs) in human blood. The device fabrication for the size based capture of rare cells in blood such as CTCs is realized in this study.

    DOI: 10.7567/JJAP.57.06JF03

    Web of Science

    Scopus

  166. Cell Deposition Microchip with Micropipette Control over Liquid Interface Motion 査読有り

    Onoshima Daisuke, Hattori Yuya, Yukawa Hiroshi, Ishikawa Kenji, Hori Masaru, Baba Yoshinobu

    CELL MEDICINE   10 巻   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1177/2155179017733152

    Web of Science

  167. A real-time simultaneous measurement on a microfluidic device for individual bacteria discrimination 査読有り

    Yasaki Hirotoshi, Yasui Takao, Yanagida Takeshi, Kaji Noritada, Kanai Masaki, Nagashima Kazuki, Kawai Tomoji, Baba Yoshinobu

    SENSORS AND ACTUATORS B-CHEMICAL   260 巻   頁: 746-752   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.snb.2018.01.079

    Web of Science

  168. In Vivo Imaging Technology of Transplanted Stem Cells Using Quantum Dots for Regenerative Medicine 査読有り

    Yukawa Hiroshi, Baba Yoshinobu

    ANALYTICAL SCIENCES   34 巻 ( 5 ) 頁: 525-532   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  169. In Vivo Imaging Technology of Transplanted Stem Cells Using Quantum Dots for Regenerative Medicine. 査読有り 国際誌

    Yukawa H, Baba Y

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   34 巻 ( 5 ) 頁: 525 - 532   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Sciences  

    Quantum dots (QDs) have excellent fluorescence properties in comparison to traditional fluorescence probes. Thus, the optical application of QDs is rapidly expanding to each field of analytical chemistry. In this review paper, we reviewed the application of QDs to regenerative medicine, especially stem cell transplantation therapy. The labeling of stem cells using QDs composed of semiconductor materials in combination with a chemical substance, poly-cationic liposome and cell penetrating peptide is reported. In addition, the influence of QD labeling on the pluripotency of stem cells is also reported. Finally, the in vivo imaging of transplanted stem cells in mice by QDs emitting fluorescence in the near-infrared region, which can be detected by in vivo fluorescence imaging systems such as IVIS and SAI-1000, is described. The future prospects for stem cell imaging technology by QDs are also discussed.

    DOI: 10.2116/analsci.17R005

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  170. Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells 査読有り

    Yukawa Hiroshi, Suzuki Kaoru, Aoki Keita, Arimoto Tomoko, Yasui Takao, Kaji Noritada, Ishikawa Tetsuya, Ochiya Takahiro, Baba Yoshinobu

    SCIENTIFIC REPORTS   8 巻   2018年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-018-24563-0

    Web of Science

  171. 尿中マイクロRNAから「がん」を特定 査読有り

    安井隆雄、馬場嘉信

    ここまで進んだ「がんの検査と診断」 ライフライン21 がんの先進医療   29 巻   頁: 30-34   2018年4月

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    記述言語:日本語  

  172. Quantitative Evaluation of Dielectric Breakdown of Silicon Micro- and Nanofluidic Devices for Electrophoretic Transport of a Single DNA Molecule 査読有り

    Sano Mamiko, Kaji Noritada, Wu Qiong, Naito Toyohiro, Yasui Takao, Taniguchi Masateru, Kawai Tomoji, Baba Yoshinobu

    MICROMACHINES   9 巻 ( 4 )   2018年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/mi9040180

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  173. 【ここまで進んだ「がんの検査と診断」】診断(肺がん・膵がん・肝がん・膀胱がん・前立腺がん) 尿中マイクロRNAから「がん」を特定

    馬場 嘉信, 安井 隆雄

    ライフライン21がんの先進医療   29 巻   頁: 30 - 34   2018年4月

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    記述言語:日本語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:(株)蕗書房  

    CiNii Article

    CiNii Books

  174. 幹細胞標識用超低毒性量子ドットFluclair™試薬の開発 査読有り

    湯川 博, 馬場嘉信

    和光純薬時報   86 巻 ( 2 ) 頁: 2 - 4   2018年4月

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    記述言語:日本語   掲載種別:研究論文(国際会議プロシーディングス)  

  175. Effect of Channel Geometry on Ionic Current Signal of a Bridge Circuit Based Microfluidic Channel 査読有り

    Yasaki Hirotoshi, Yasui Takao, Yanagida Takeshi, Kaji Noritada, Kanai Masaki, Nagashima Kazuki, Kawai Tomoji, Baba Yoshinobu

    CHEMISTRY LETTERS   47 巻 ( 3 ) 頁: 350-353   2018年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1246/cl.171139

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  176. Facile fabrication of a poly(ethylene terephthalate) membrane filter with precise arrangement of through-holes 査読有り

    Kihara Naoto, Odaka Hidefumi, Kuboyama Daiki, Onoshima Daisuke, Ishikawa Kenji, Baba Yoshinobu, Hori Masaru

    JAPANESE JOURNAL OF APPLIED PHYSICS   57 巻 ( 3 )   2018年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.7567/JJAP.57.037001

    Web of Science

  177. Robust Ionic Current Sensor for Bacterial Cell Size Detection 査読有り

    Yasaki Hirotoshi, Shimada Taisuke, Yasui Takao, Yanagida Takeshi, Kaji Noritada, Kanai Masaki, Nagashima Kazuki, Kawai Tomoji, Baba Yoshinobu

    ACS SENSORS   3 巻 ( 3 ) 頁: 574-579   2018年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acssensors.8b00045

    Web of Science

  178. Quantum Dot-Peptide Nanoassembly on Mesoporous Silica Nanoparticle for Biosensing 査読有り 国際誌

    Pillai Sreenadh Sasidharan, Yukawa Hiroshi, Onoshima Daisuke, Biju Vasudevanpillai, Baba Yoshinobu

    NANO HYBRIDS AND COMPOSITES   19 巻   頁: 55-72 - 72   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.4028/www.scientific.net/NHC.19.55

    Web of Science

  179. Nanobiodevice-Based Cancer Theranostics

    Baba Yoshinobu

    CANCER SCIENCE   109 巻   頁: 146-146   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  180. Application of biomaterials in interdisciplinary approaches for cancer research

    Baba Yoshinobu, Kano Mitsunobu

    CANCER SCIENCE   109 巻   頁: 146-146   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  181. Application of biomaterials in interdisciplinary approaches for cancer research

    Baba Yoshinobu, Kano Mitsunobu

    CANCER SCIENCE   109 巻   頁: 146-146   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  182. Nanobiodevice-Based Cancer Theranostics

    Baba Yoshinobu

    CANCER SCIENCE   109 巻   頁: 146-146   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  183. Collection and Sensing of PM2.5 in Microfluidic Devices

    Shimada Taisuke, Yasaki Hirotoshi, Yasui Takao, Kaji Noritada, Baba Yoshinobu

    2018 IEEE 12TH INTERNATIONAL CONFERENCE ON NANO/MOLECULAR MEDICINE AND ENGINEERING (IEEE - NANOMED)     頁: 240-243   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  184. PM<sub>2.5</sub> Particle Detection in a Microfluidic Device by Using Ionic Current Sensing. 査読有り

    Shimada T, Yasaki H, Yasui T, Yanagida T, Kaji N, Kanai M, Nagashima K, Kawai T, Baba Y

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   34 巻 ( 12 ) 頁: 1347 - 1349   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Sciences  

    We have demonstrated a PM 2.5 analysis method that adds information on the number concentration and size by using microfluidic-based ionic current sensing with a bridge circuit. The bridge circuit allows for suppression of the background current and the detection of small PM 2.5 particles, even if a relatively large micropore is used. This is the first demonstration of the detection of PM 2.5 particles via ionic current sensing; our method enables analyses of both the number concentration and size.

    DOI: 10.2116/analsci.18C018

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  185. Exosome isolation toward cancer diagnosis using glass filter with nanoporous structure 査読有り 国際誌

    Keita Aoki, Hiroshi Yukawa, Daisuke Onoshima, Shuji Yamazaki, Naoto Kihara, Ryohei Koguchi, Kumiko Takahashi, Hidefumi Odaka, Kenji Ishikawa, Masaru Hori, Yoshinobu Baba

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   3 巻   頁: 1409 - 1410   2018年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. We present a study of centrifugal filtration device toward easy-to-use approach of non-invasive liquid biopsy for cancer diagnosis by cell-derived nanoscale vesicles (exosomes). A phase-dispersed glass filter having nanoporous structure was embedded into a spin column to trap exosomes with small centrifuge. This device enabled over 90% exosome isolation from biological samples within 10 minutes.

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  186. Exosome isolation toward cancer diagnosis using glass filter with nanoporous structure 査読有り 国際誌

    Aoki K.

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   3 巻   頁: 1409 - 1410   2018年

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    掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018  

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  187. Cell deformability measurement device for labeled-free cancer cells discriminating using ionic current detection 査読有り 国際誌

    Suzuki T.

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   1 巻   頁: 347 - 348   2018年

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    掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018  

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  188. Cell deformability measurement device for labeled-free cancer cells discriminating using ionic current detection 査読有り 国際誌

    T. Suzuki, N. Kaji, H. Yasaki, T. Yasui, Y. Baba

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   1 巻   頁: 347 - 348   2018年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    Copyright© (2018) by Chemical and Biological Microsystems Society.All rights reserved. In this study, we have developed ionic current detection device that can discriminate cells by measurement of cell deformability for detecting circulating tumor cells (CTC). We successfully discriminated CTC model from leukocyte model without any fluorescent labels by using the device. Moreover, we could detect relatively small CTCs compared to blood cells, which are difficult to detect by conventional filter devices. This device is expected as a new cancer diagnostic device that is simple and highly sensitive.

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  189. Microheater-nanowires device for detection of cell-free circulating methylated DNA 査読有り 国際誌

    Hiromi Takahashi, Takao Yasui, Keiko Shinjo, Noritada Kaji, Akimitsu Okamoto, Yoshinobu Baba

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   4 巻   頁: 1886 - 1888   2018年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. Detecting of cell-free circulating methylated DNA could be a promising method for early diagnosis of cancer. Here we have developed the microheater - zinc oxide nanowires (ZnO-NWs) structures embedded in the microchannel device for capturing DNA via electrostatic interaction. We found that 60% of DNA could be captured on ZnO-NWs. Additionally, the microheater, which constantly enables to generate heat, can denature double stranded DNA (dsDNA) to single stranded DNA (ssDNA). Finally, ICON Probe, which is an artificial ssDNA probe for DNA methylation, was introduced inside a device to bind to ssDNA for DNA methylation detection.

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  190. Selective molecular recognition using molecular fingerprinted nanowires 査読有り 国際誌

    Horiuchi M.

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   2 巻   頁: 659 - 660   2018年

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    掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018  

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  191. Selective molecular recognition using molecular fingerprinted nanowires 査読有り 国際誌

    Masafumi Horiuchi, Takao Yasui, Kazuki Nagashima, Takeshi Yanagida, Yoshinobu Baba

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   2 巻   頁: 659 - 660   2018年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. Molecular recognition methods such as those found in antigen-antibody reactions are attracting attention. We demonstrated more selective molecular recognition through focusing on not only intermolecular interaction but also the shape of the molecules. Here, we fabricated molecular shapes of aniline on the titanium oxide thin film on the core-shell structure of zinc oxide/titanium oxide nanowires, and recognized aniline, selectively, using the aniline molecular shaped nanowires.

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  192. PM2.5 analysis in liquid phase via water film-based collection and microfluidics-based electrical detection 査読有り 国際誌

    Shimada T.

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   1 巻   頁: 270 - 271   2018年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018  

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  193. PM2.5 analysis in liquid phase via water film-based collection and microfluidics-based electrical detection 査読有り 国際誌

    Taisuke Shimada, Hirotoshi Yasaki, Takao Yasui, Akihide Hibara, Takeshi Yanagida, Noritada Kaji, Masaki Kanai, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   1 巻   頁: 270 - 271   2018年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    Copyright© (2018) by Chemical and Biological Microsystems Society.All rights reserved. Toward evaluation of health risk of particulate matter with size less than or equal to 2.5 µm (PM2.5), we propose a methodology for realizing the PM2.5 analysis in liquid phase via water film-based collection and microfluidics-based electrical detection. A hydrophilic nanowire surface was utilized in the collection for forming a water film, which enabled continuous collection of air-floating PM2.5 into liquid phase and followed-by detection. The collected PM2.5 was electrically detected and analyzed via ionic current sensing with a micropore. We believe proposed PM2.5 analysis methodology in liquid phase would allow to realize health risk evaluation and reduce health damage.

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  194. Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification 査読有り 国際誌

    Onoshima D.

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   3 巻   頁: 1291 - 1293   2018年

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    掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018  

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  195. Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification 査読有り 国際誌

    Daisuke Onoshima, Daiki Kuboyama, Naoto Kihara, Hiromasa Tanaka, Tetsunari Hase, Hiroshi Yukawa, Kenji Ishikawa, Hidefumi Odaka, Yoshinori Hasegawa, Masaru Hori, Yoshinobu Baba

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   3 巻   頁: 1291 - 1293   2018年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

    Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. We present a study of genomic profiling for circulating tumor cells (CTCs) using a blood microfiltration system. DNA amplification of CTCs was performed with a microfilter for single cancer cells in a clinical setting. This strategy was successfully used to detect the driver mutations in epidermal growth factor (EGF) receptor oncogenes by quantitative PCR and characterize the dosing effect of molecularly targeted drug for lung cancer.

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  196. Microheater-nanowires device for detection of cell-free circulating methylated DNA 査読有り 国際誌

    Takahashi H.

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   4 巻   頁: 1886 - 1888   2018年

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    掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018  

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  197. Discriminating single-bacterial shape using low-aspect-ratio pores 査読有り

    Tsutsui Makusu, Yoshida Takeshi, Yokota Kazumichi, Yasaki Hirotoshi, Yasui Takao, Arima Akihide, Tonomura Wataru, Nagashima Kazuki, Yanagida Takeshi, Kaji Noritada, Taniguchi Masateru, Washio Takashi, Baba Yoshinobu, Kawai Tomoji

    SCIENTIFIC REPORTS   7 巻   2017年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-017-17443-6

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  198. Unveiling massive numbers of cancer-related urinary-microRNA candidates via nanowires 査読有り

    Yasui Takao, Yanagida Takeshi, Ito Satoru, Konakade Yuki, Takeshita Daiki, Naganawa Tsuyoshi, Nagashima Kazuki, Shimada Taisuke, Kaji Noritada, Nakamura Yuta, Thiodorus Ivan Adiyasa, He Yong, Rahong Sakon, Kanai Masaki, Yukawa Hiroshi, Ochiya Takahiro, Kawai Tomoji, Baba Yoshinobu

    SCIENCE ADVANCES   3 巻 ( 12 )   2017年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1126/sciadv.1701133

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  199. Understanding the formation mechanism of lipid nanoparticles in microfluidic devices with chaotic micromixers 査読有り

    M. Maeki, Y. Fujishima, Y. Sato, T. Yasui, N. Kaji, A. Ishida, H. Tani, Y. Baba, H. Harashima, M. Tokeshi

    PLoS One     2017年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1371/journal.pone.0187962

  200. Development of the immuno-wall device for rapid, low-cost detection of EGFR mutations in tumor samples from patients with lung cancer

    Yogo N., Hase T., Kasama T., Ozawa N., Sato M., Kaji N., Tokeshi M., Baba Y., Hasegawa Y.

    ANNALS OF ONCOLOGY   28 巻   頁: .   2017年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  201. Development of the immuno-wall device for rapid, low-cost detection of EGFR mutations in tumor samples from patients with lung cancer

    Yogo N, Hase T, Kasama T, Ozawa N, Sato M, Kaji N, Tokeshi M, Baba Y, Hasegawa Y

    ANNALS OF ONCOLOGY   28 巻   頁: .   2017年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  202. NANOBIODEVICES AND AI FOR SOCIETY 5.0: SUPER SMART SOCIETY 査読有り

    Y. Baba

    Micro Total Analysis Systems 2017     頁: 1-2   2017年10月

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    記述言語:英語  

  203. PM2.5 collection into aqueous solution via hydrophilic oxide nanowire surface 査読有り

    T. Shimada, T. Yasui, A. Hibara, H. Yasaki, T. Yanagida, M. Hara, N. Kaji, M. Kanai, K. Nagashima, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2017     頁: 1433-1434   2017年10月

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    記述言語:英語  

  204. Nanowires enabling cancer diagnosis from 1 mL urine 招待有り 査読有り

    T. Naganawa, T. Yasui, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, H. Yukawa, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2017     頁: 1271-1272   2017年10月

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    記述言語:英語  

  205. Extracellular vesicles separation by electroosmotic flow-driven deterministic lateral displacement in nanopillar chips 査読有り

    N. Kaji, Y. Hattori, H. Yasaki, T. Yasui, Y. Baba

    Micro Total Analysis Systems 2017     頁: 904-905   2017年10月

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    記述言語:英語  

  206. A zinc oxide nanowires devices for extracellular vesicles isolation and purification 査読有り

    I. A. Thiodorus, N. Kaji, T. Yasui, Y. Baba

    Micro Total Analysis Systems 2017     頁: 886-887   2017年10月

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    記述言語:英語  

  207. LEUKOCYTE DEPLETION AND SIZE-BASED ENRICHMENT OF CIRCULATING TUMOR CELLS WITH PRESSURE-SENSING MICROFILTRATION SYSTEM 査読有り

    D. Kuboyama, D. Onoshima, N. Kihara, H. Tanaka, T. Hase, H. Yukawa, K. Ishikawa, H. Odaka, Y. Hasegawa, M. Hori, Y. Baba

    Micro Total Analysis Systems 2017     頁: 882-883   2017年10月

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    記述言語:英語  

  208. Millisecond mechanophenotyping of stem cells by electrical detection in microfluidic constrictions 査読有り

    N. Kaji, S. Ito, H. Yasaki, T. Yasui, H. Yukawa, Y. Baba

    Micro Total Analysis Systems 2017     頁: 832-833   2017年10月

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    記述言語:英語  

  209. CELL DEPOSITION AND ISOLATION WITH MICROPIPETTE CONTROL OVER LIQUID INTERFACE MOTION IN MICROFLUIDIC CHANNEL 査読有り

    Daisuke Onoshima, Hiroshi Yukawa, Yuya Hattori, Kenji Ishikawa, Masaru Hori, Yoshinobu Baba

    Micro Total Analysis Systems 2017     頁: 679-680   2017年10月

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    記述言語:英語  

  210. A zinc oxide nanowires devices for extracellular vesicles isolation and purification 査読有り

    I. A. Thiodorus, N. Kaji, T. Yasui, Y. Baba

    Micro Total Analysis Systems 2017     頁: 886-887   2017年10月

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    記述言語:英語   掲載種別:研究論文(その他学術会議資料等)  

  211. Extracellular vesicles separation by electroosmotic flow-driven deterministic lateral displacement in nanopillar chips 査読有り

    N. Kaji, Y. Hattori, H. Yasaki, T. Yasui, Y. Baba

    Micro Total Analysis Systems 2017     頁: 904-905   2017年10月

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    記述言語:英語   掲載種別:研究論文(その他学術会議資料等)  

  212. CELL DEPOSITION AND ISOLATION WITH MICROPIPETTE CONTROL OVER LIQUID INTERFACE MOTION IN MICROFLUIDIC CHANNEL 査読有り

    Daisuke Onoshima, Hiroshi Yukawa, Yuya Hattori, Kenji Ishikawa, Masaru Hori, Yoshinobu Baba

    Micro Total Analysis Systems 2017     頁: 679-680   2017年10月

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    記述言語:英語   掲載種別:研究論文(その他学術会議資料等)  

  213. LEUKOCYTE DEPLETION AND SIZE-BASED ENRICHMENT OF CIRCULATING TUMOR CELLS WITH PRESSURE-SENSING MICROFILTRATION SYSTEM 査読有り

    D. Kuboyama, D. Onoshima, N. Kihara, H. Tanaka, T. Hase, H. Yukawa, K. Ishikawa, H. Odaka, Y. Hasegawa, M. Hori, Y. Baba

    Micro Total Analysis Systems 2017     頁: 882-883   2017年10月

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    記述言語:英語   掲載種別:研究論文(その他学術会議資料等)  

  214. PM2.5 collection into aqueous solution via hydrophilic oxide nanowire surface 査読有り

    T. Shimada, T. Yasui, A. Hibara, H. Yasaki, T. Yanagida, M. Hara, N. Kaji, M. Kanai, K. Nagashima, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2017     頁: 1433-1434   2017年10月

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    記述言語:英語   掲載種別:研究論文(その他学術会議資料等)  

  215. Nanowires enabling cancer diagnosis from 1 mL urine 招待有り 査読有り

    T. Naganawa, T. Yasui, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, H. Yukawa, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2017     頁: 1271-1272   2017年10月

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    記述言語:英語   掲載種別:研究論文(その他学術会議資料等)  

  216. NANOBIODEVICES AND AI FOR SOCIETY 5.0: SUPER SMART SOCIETY 査読有り

    Y. Baba

    Micro Total Analysis Systems 2017     頁: 1-2   2017年10月

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    記述言語:英語   掲載種別:研究論文(その他学術会議資料等)  

  217. Millisecond mechanophenotyping of stem cells by electrical detection in microfluidic constrictions 査読有り

    N. Kaji, S. Ito, H. Yasaki, T. Yasui, H. Yukawa, Y. Baba

    Micro Total Analysis Systems 2017     頁: 832-833   2017年10月

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    記述言語:英語   掲載種別:研究論文(その他学術会議資料等)  

  218. Optimization of the nanofluidic design for label-free detection of biomolecules using a nanowall array 査読有り

    Taiga Ajiri, Takao Yasui, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi

    Sensors and Actuators B: Chemical   250 巻   頁: pp.39-43   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.snb.2017.04.150

  219. 尿を使った疾病診断を目指したナノワイヤ空間の創製 査読有り

    安井隆雄、加地範匡、馬場嘉信

    化学と工業   68 巻 ( 10 ) 頁: 768-772   2017年10月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  220. 尿を使った疾病診断を目指したナノワイヤ空間の創製 (特集 ナノ空間材料の創成と応用)

    安井 隆雄, 加地 範匡, 馬場 嘉信

    化學工業   68 巻 ( 10 ) 頁: 768 - 772   2017年10月

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    記述言語:日本語   掲載種別:研究論文(その他学術会議資料等)   出版者・発行元:小峰工業出版  

    CiNii Article

    CiNii Books

  221. Substantial Expansion of Detectable Size Range in Ionic Current Sensing through Pores by Using a Microfluidic Bridge Circuit 査読有り

    Hirotoshi Yasaki, Takao Yasui, Takeshi Yanagida, Noritada Kaji, Masaki Kanai, Kazuki Nagashima, Tomoji Kawai, and Yoshinobu Baba

    J. Am. Chem. Soc.   139 巻 ( 40 ) 頁: 14137–14142   2017年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/jacs.7b06440

  222. 量子ドットによる移植幹細胞in vivo 蛍光イメージング 査読有り

    湯川博、馬場嘉信

      24 巻 ( 2 ) 頁: 81-86   2017年8月

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    記述言語:日本語  

  223. Transplantation of bioengineered rat lungs recellularized with endothelial and adipose-derived stromal cells 査読有り

    Ryoichiro Doi, Tomoshi Tsuchiya, Norisato Mitsutake, Satoshi Nishimura, Mutsumi Matsuu-Matsuyama, Yuka Nakazawa, Tomoo Ogi, Sadanori Akita, Hiroshi Yukawa, Yoshinobu Baba, Naoya Yamasaki, Keitaro Matsumoto, Takuro Miyazaki, Ryotaro Kamohara, Go Hatachi, Hideyori Sengyoku, Hironosuke Watanabe, Tomohiro Obata, Laura E. Niklason, and Takeshi Nagayasu

    Scientific Reports (Nature Publishing Group)     2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-017-09115-2

  224. Transplantation of bioengineered rat lungs recellularized with endothelial and adipose-derived stromal cells

    Doi Ryoichiro, Tsuchiya Tomoshi, Mitsutake Norisato, Nishimura Satoshi, Matsuu-Matsuyama Mutsumi, Nakazawa Yuka, Ogi Tomoo, Akita Sadanori, Yukawa Hiroshi, Baba Yoshinobu, Yamasaki Naoya, Matsumoto Keitaro, Miyazaki Takuro, Kamohara Ryotaro, Hatachi Go, Sengyoku Hideyori, Watanabe Hironosuke, Obata Tomohiro, Niklason Laura E., Nagayasu Takeshi

    SCIENTIFIC REPORTS   7 巻   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-017-09115-2

    Web of Science

  225. Perioperative airway management of a 16-year-old boy with progressive airway obstruction due to juvenile nasopharyngeal angiofibroma

    Kamiyama Kei, Satomoto Maiko, Minami Kotaro, Baba Yukiko, Makita Koshi

    CLINICAL CASE REPORTS   5 巻 ( 8 ) 頁: 1274 - 1276   2017年8月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/ccr3.1056

    Web of Science

  226. Time-to-Furosemide Treatment and Mortality in Patients Hospitalized With Acute Heart Failure

    Matsue Y.

    Journal of the American College of Cardiology   69 巻 ( 25 ) 頁: 3042-3051   2017年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.jacc.2017.04.042

    Scopus

  227. Stacking-cyclodextrin-microchip electrokinetic chromatographic determination of gabapentinoid drugs in pharmaceutical and biological matrices 査読有り

    Abdallah Zeid, Noritada Kaji, Jenny Jeehan Nasr,Fathalla Belal,Yoshinobu Baba, Mohamed Walash

    Journal of Chromatography A   1503 巻 ( 23 ) 頁: pp.65-75   2017年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.chroma.2017.04.049

  228. Nanostructures Integrated with a Nanochannel for Slowing Down DNA Translocation Velocity for Nanopore Sequencing 査読有り

    Xiaoyin SUN, Takao YASUI, Takeshi YANAGIDA, Noritada KAJI, Sakon RAHONG, Masaki KANAI, Kazuki NAGASHIMA, Tomoji KAWAI, Yoshinobu BABA

    Analytical Sciences   33 巻 ( 6 ) 頁: pp.75-738   2017年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 0.2116/analsci.33.735

  229. Double-hydrophobic elastin-like polypeptides with added functional motifs: Self-assembly and cytocompatibility 査読有り

    Duc H. T. Le, Yoko Tsutsui, Ayae Sugawara-Narutaki, Hiroshi Yukawa, Yoshinobu Baba, Chikara Ohtsuki

    Journal of Biomedical Materials Research Part A     2017年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/jbm.a.36105

  230. Nanostructures Integrated with a Nanochannel for Slowing Down DNA Translocation Velocity for Nanopore Sequencing 査読有り

    Xiaoyin Sun, Takao Yasui, Takeshi Yanagida, Noritada Kaji, Sakon Rahong, Masaki Kanai, Kazuki Nagashima, Tomoji Kawal, Yoshinobu Baba

    ANALYTICAL SCIENCES   33 巻 ( 6 ) 頁: 735 - 738   2017年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC ANALYTICAL CHEMISTRY  

    Here, we developed a device integrated with a nanochannel and nanostructures to slow DNA translocation velocity. We found that translocation velocity of a single DNA molecule inside a nanochannel was decreased by pre-elongating it using some nanostructures, such as a shallow channel or nanopillars. This decrease of the translocation velocity was associated with the DNA mobility change, which is an intrinsic parameter of DNA molecules and unaffected by an electric field.

    Web of Science

  231. A millisecond micro-RNA separation technique by a hybrid structure of nanopillars and nanoslits 査読有り

    Qiong Wu, Noritada Kaji,Takao Yasui, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Kazuki Nagashima,Manabu Tokeshi,Tomoji Kawai, and Yoshinobu Baba

    Scientific Reports   7 巻   2017年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/srep43877

  232. A millisecond micro-RNA separation technique by a hybrid structure of nanopillars and nanoslits

    Wu Qiong, Kaji Noritada, Yasui Takao, Rahong Sakon, Yanagida Takeshi, Kanai Masaki, Nagashima Kazuki, Tokeshi Manabu, Kawai Tomoji, Baba Yoshinobu

    SCIENTIFIC REPORTS   7 巻   2017年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/srep43877

    Web of Science

  233. Transduction Function of a Magnetic Nanoparticle TMADM for Stem-Cell Imaging with Quantum Dots 査読有り

    Yusuke OGIHARA, Hiroshi YUKAWA, Daisuke ONOSHIMA, Yoshinobu BABA

    Analytical Sciences   33 巻 ( 2 ) 頁: pp.143-146   2017年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 0.2116/analsci.33.143

  234. Förster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing 査読有り

    Sreenadh Sasidharan PILLAI, Hiroshi YUKAWA, Daisuke ONOSHIMA, Vasudevanpillai BIJU, Yoshinobu BABA

    Analytical Sciences   33 巻 ( 2 ) 頁: 137 - 142   2017年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Sciences  

    The steady state and time-resolved photoluminescence quenching of streptavidin modified CdSe/ZnS quantum dots (QDs) instigated by biotin-peptide-BHQ-1 (biotin-pep-BHQ-1) molecule was investigated. Here, we have achieved an efficient photoluminescence (PL) quenching of QDs with the conjugation of dark quencher (black hole quencher-BHQ) molecules intermediated with the GPLGVRGK peptide. The luminescence of streptavidin-QDs585 was decreased upon titration with a nano molar concentration of the biotin-GPLGVRGK-BHQ-1 molecule. It has been suggested that the decrease of QDs PL occurred through a Förster resonance energy transfer (FRET) mechanism from the analysis of steady state photoluminescence intensity measurements as well as time resolved lifetime measurements of streptavidin-QDs and QDs- (pep-BHQ-1) n conjugates. The sequence of intermediate peptide GPLG↓VRGK can act as a target material for matrix metalloproteinases-2 (MMP-2) produced by cancer cells at its Gly and Val region, shown by the down-headed arrow. Interestingly, here the reported self-assembled QDs-(pep-BHQ-1) n conjugates could detect the presence MMP-2 at a detection limit of 1 ng/mL with a clear luminescence recovery.

    DOI: 10.2116/analsci.33.137

    Web of Science

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  235. Transduction Function of a Magnetic Nanoparticle TMADM for Stem-Cell Imaging with Quantum Dots 査読有り

    Yusuke Ogihara, Hiroshi Yukawa, Daisuke Onoshima, Yoshinobu Baba

    ANALYTICAL SCIENCES   33 巻 ( 2 ) 頁: 143 - 146   2017年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC ANALYTICAL CHEMISTRY  

    We investigated the transduction function of a cationic dextran hydroxypropyltrimethyl ammonium chloride-coated magnetic iron oxide nanoparticle (TMADM-03) for transducing quantum dots (QDs) into adipose tissue-derived stem cells (ASCs). As a result, the fluorescence intensity of ASCs labeled with QDs using TMADM-03 was much higher than that of QDs only labeling. These data suggest that TMADM-03 can be useful as a transduction agent for QDs in stem-cell imaging.

    Web of Science

  236. In vivo fluorescence imaging and the diagnosis of stem cells using quantum dots for regenerative medicine 査読有り

    H. Yukawa, Y. Baba

    analytical chemistry   89 巻 ( 5 ) 頁: PP.2671-2681   2017年1月

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    記述言語:英語  

    DOI: 10.1021/acs.analchem.6b04763

  237. NIR-II近赤外領域における移植幹細胞in vivo蛍光イメージング 査読有り

    湯川博、小林香央里、新岡宏彦、亀山達矢、佐藤和秀、鳥本司、石川哲也、馬場嘉信

    バイオインダストリー   34 巻   頁: pp.27-34   2017年1月

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    記述言語:日本語  

  238. Immunomodulatory effects of adipose tissue-derived stem cells on concanavalin A-induced acute liver injury in mice 査読有り

    Yoshizumi Y., Yukawa H*., Iwaki R., Fujinaka S., Kanou A., Kanou Y., Yamada T., Nakagawa S., Ohara T., Nakagiri K., Ogihara Y., Tsutsui Y., Hayashi Y., Ishigami M., Baba Y., Ishikawa T.

    Cell Medicine   91 巻 ( 1-2 ) 頁: pp.21-23   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  239. Immunomodulatory effects of adipose tissue-derived stem cells on concanavalin A-induced acute liver injury in mice 査読有り

    Yoshizumi Y, Yukawa H, Iwaki R, Fujinaka S, Kanou A, Kanou Y, Yamada T, Nakagawa S, Ohara T, Nakagiri K, Ogihara Y, Tsutsui Y, Hayashi Y, Ishigami M, Baba Y, Ishikawa T

    Cell Medicine   91 巻 ( 1-2 ) 頁: pp.21-23   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  240. Labeling and in vivo Visualization of Transplanted Adipose Tissue-Derived Stem Cells with Safe Cadmium-Free Aqueous ZnS coating of ZnS-AgInS2 Nanoparticles 査読有り

    .Y. Ogihara, H. Yukawa, T. Kameyama, H. Nishi, D. Onoshima, T. Ishikawa, T. Torimoto, Y. Baba

    Scientific Reports   7 巻   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/srep40047

  241. Labeling and in vivo visualization of transplanted adipose tissue-derived stem cells with safe cadmium-free aqueous ZnS coating of ZnS-AgInS2 nanoparticles

    Ogihara Yusuke, Yukawa Hiroshi, Kameyama Tatsuya, Nishi Hiroyasu, Onoshima Daisuke, Ishikawa Tetsuya, Torimoto Tsukasa, Baba Yoshinobu

    SCIENTIFIC REPORTS   7 巻   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/srep40047

    Web of Science

  242. DDSとナノテクノロジーがもたらす超スマート社会 査読有り

    馬場嘉信

    DDS学会誌   32 巻 ( 1 ) 頁: 8   2017年1月

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    担当区分:筆頭著者   記述言語:日本語  

    DOI: 10.2745/dds.32.8

  243. INSECTS REPORTS セミの翅の物理的防御機構を模倣したナノワイヤ構造体の創製

    安井 隆雄, 米勢 明弘, 加地 範匡, 柳田 剛, 川合 知二, 馬場 嘉信

    昆虫と自然   52 巻 ( 1 ) 頁: 35 - 37   2017年1月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ニューサイエンス社  

    CiNii Article

    CiNii Books

  244. Effect of DNA Methylation on the Velocity of DNA Translocation through a Nanochannel. 査読有り

    Sun X, Yasui T, Yanagida T, Kaji N, Rahong S, Kanai M, Nagashima K, Kawai T, Baba Y

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   33 巻 ( 6 ) 頁: 727 - 730   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Sciences  

    Here, we report the effect of DNA methylation on the velocity of DNA translocation through a nanochannel, as determined by measuring differences in translocation velocities between methylated and non-methylated DNA molecules. We found that the velocity of translocation of methylated DNA was faster than that of non-methylated DNA, which we attributed to variation in the coefficients of diffusion and friction with the nanochannel wall, due to the increased molecular weight and stiffness, respectively, of methylated DNA.

    DOI: 10.2116/analsci.33.727

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    CiNii Article

  245. A Single Bacterium and Mammalian Cell Analysis by Ionic Current Measurements in a Microchannel

    Kaji N., Sano M., Ito S., Yasaki H., Yasui T., Yukawal H., Baba Y.

    2017 IEEE INTERNATIONAL ELECTRON DEVICES MEETING (IEDM)     頁: .   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  246. Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-Immobilized Microbeads Retained in Porous Hydrogel Micropillars

    Kasama Toshihiro, Kaji Noritada, Tokeshi Manabu, Baba Yoshinobu

    MICROCHIP DIAGNOSTICS: METHODS AND PROTOCOLS   1547 巻   頁: 49-56   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/978-1-4939-6734-6_4

    Web of Science

  247. Microfluidic DNA Stretching Device for Single-Molecule Diagnostics

    Onoshima Daisuke, Baba Yoshinobu

    MICROCHIP DIAGNOSTICS: METHODS AND PROTOCOLS   1547 巻   頁: 105-111   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/978-1-4939-6734-6_8

    Web of Science

  248. Nanostructures Integrated with a Nanochannel for Slowing Down DNA Translocation Velocity for Nanopore Sequencing. 査読有り

    Sun X, Yasui T, Yanagida T, Kaji N, Rahong S, Kanai M, Nagashima K, Kawai T, Baba Y

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   33 巻 ( 6 ) 頁: 735 - 738   2017年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Sciences  

    Here, we developed a device integrated with a nanochannel and nanostructures to slow DNA translocation velocity. We found that translocation velocity of a single DNA molecule inside a nanochannel was decreased by pre-elongating it using some nanostructures, such as a shallow channel or nanopillars. This decrease of the translocation velocity was associated with the DNA mobility change, which is an intrinsic parameter of DNA molecules and unaffected by an electric field.

    DOI: 10.2116/analsci.33.735

    Web of Science

    Scopus

    PubMed

    CiNii Article

  249. Transduction Function of a Magnetic Nanoparticle TMADM for Stem-Cell Imaging with Quantum Dots. 査読有り

    Ogihara Y, Yukawa H, Onoshima D, Baba Y

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   33 巻 ( 2 ) 頁: 143 - 146   2017年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Sciences  

    We investigated the transduction function of a cationic dextran hydroxypropyltrimethyl ammonium chloride-coated magnetic iron oxide nanoparticle (TMADM-03) for transducing quantum dots (QDs) into adipose tissue-derived stem cells (ASCs). As a result, the fluorescence intensity of ASCs labeled with QDs using TMADM-03 was much higher than that of QDs only labeling. These data suggest that TMADM-03 can be useful as a transduction agent for QDs in stem-cell imaging.

    DOI: 10.2116/analsci.33.143

    Web of Science

    Scopus

    PubMed

    CiNii Article

  250. 量子ドットによる移植幹細胞<i>in vivo</i>蛍光イメージング

    湯川 博, 馬場 嘉信

    Organ Biology   24 巻 ( 2 ) 頁: 201 - 206   2017年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:一般社団法人 日本臓器保存生物医学会  

    Quantum dots (QDs) have excellent fluorescence properties in comparison to traditional fluorescence probes. Thus, the optical application of QDs is in rapid expansion to each field of analytical chemistry. In this paper, we described about the application of QDs to regenerative medicine, especially stem cell transplantation therapy. Specifically, labeling technologies of stem cells by QDs composed of semiconductor materials in combination with poly-cationic liposome (Lipofectamine<sup>&reg;</sup>) or cell penetrating peptide (octa-arginine) were reviewed. Moreover, <i>in vivo</i> imaging of transplanted stem cells in mice by QDs emitting fluorescence in near-infrared region, which could be detected by fluorescence <i>in vivo</i> imaging machines such as IVIS imaging system, was reported.

    DOI: 10.11378/organbio.24.201

    CiNii Article

  251. A Single Bacterium and Mammalian Cell Analysis by Ionic Current Measurements in a Microchannel

    Kaji N, Sano M, Ito S, Yasaki H, Yasui T, Yukawal H, Baba Y

    2017 IEEE INTERNATIONAL ELECTRON DEVICES MEETING (IEDM)     頁: .   2017年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  252. セミの翅の物理的防御機構を模倣したナノワイヤ構造体の創製 査読有り

    安井隆雄, 米勢明弘, 加地範匡, 柳田剛, 川合知二, 馬場嘉信

    昆虫と自然   52 巻   頁: pp.35-37   2016年12月

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    記述言語:日本語  

  253. Microfluidic Transport Through Microsized Holes Treated by Nonequilibrium Atmospheric-Pressure Plasma 査読有り 国際誌

    Ito Takumi, Ishikawa Kenji, Onoshima Daisuke, Kihara Naoto, Tatsukoshi Kentaro, Odaka Hidefumi, Hashizume Hiroshi, Tanaka Hiromasa, Yukawa Hiroshi, Takeda Keigo, Kondo Hiroki, Sekine Makoto, Baba Yoshinobu, Hori Masaru

    IEEE TRANSACTIONS ON PLASMA SCIENCE   44 巻 ( 12 ) 頁: 3060 - 3065   2016年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:IEEE Transactions on Plasma Science  

    In the field of microfluidics, it is possible to facilitate liquid transport through microsized holes with large slip lengths by lowering the friction at the interface between the flow and the inner surface of the holes. In this paper, we discuss the use of nonequilibrium atmospheric-pressure plasma to modify the surface wettability of microsized holes in glass substrates that are similar to those used as flow channels in glass microfiltration devices. In our experiments, liquid transport flows were driven by internal Laplace pressure differences based on the surface tensions of droplets placed on the front and back sides of the tested substrates.

    DOI: 10.1109/TPS.2016.2571721

    Web of Science

    Scopus

  254. Identifying DNA methylation in a nanochannel 査読有り

    X. Sun, T. Yasui, T. Yanagida, N. Kaji, S. Rahong, M. Kanai, K. Nagashima, T. Kawai, Y. Baba

    Sci. Technol. Adv. Mater.   17 巻 ( 1 ) 頁: pp.644-649   2016年10月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

  255. MICROFLUIDIC LONG-PORE-CHANNEL TO HIGHLIGHT BACTERIA CONTENTS 査読有り

    H. Yasaki, T. Yasui1,2, T. Yanagida, N. Kaji, M. Kanai, M. Fukuyama, K. Nagashima, T. Kawai, and Y. Baba

    Micro Total Analysis Systems 2016     頁: 100-101   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  256. MICRO SAND TIMER IN GLASS MEMBRANE DEVICE SEPARATES SINGLE CIRCULATING TUMOR CELLS IN BLOOD 査読有り

    D. Kuboyama, D. Onoshima, H. Yukawa, M. Tanaka, K. Ishikawa4, M. Hori1, and Y. Baba

    Micro Total Analysis Systems 2016     頁: 297-298   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  257. NANOWIRES FOR EARLY CANCER AND DIABETES DIAGNOSIS VIA MICRO-RNA DETECTION IN URINE EXTRACELLULAR VESICLES 査読有り

    T. Yasui, D. Takeshita, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, H. Yukawa1, T. Kawai, and Y. Baba

    Micro Total Analysis Systems 2016     頁: 108-109   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  258. MILLISECOND MEASUREMENT OF SINGLE CELL SIZE AND DEFORMABILITY BY ELECTRICAL DETECTION IN MICROFLUIDIC DEVICE WITH TWO CONSECUTIVE CONSTRICTIONS 査読有り

    N. Kaji, M. Sano, H. Yasaki, T. Yasui, and Y. Baba

    Micro Total Analysis Systems 2016     頁: 341-342   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  259. WATER FILM DEVICE FOR CONTINUOUS PARTICULATE MATTER COLLECTION 査読有り

    T. Shimada, T. Yasui, A. Hibara, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, T. Kawai, and Y. Baba

    Micro Total Analysis Systems 2016     頁: 1473-1474   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  260. A HIGH-SENSITIVITY CYCLODEXTRIN-MICROCHIP ELECTROPHORESIS SIMULTANEOUS ANALYSIS OF GABAPENTIN AND PREGABALIN 査読有り

    A.M. Zeid, J. Nasr, N. Kaji, F.F. Belal, M.I. Walash, and Y. Baba

    Micro Total Analysis Systems 2016     頁: 1457-1458   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  261. EFFECTS OF THE GROOVED STRUCTURES AND THE ETHANOL CONCENTRATION ON THE SMALL-SIZED LIPID NANOPARTICLES FORMATION 査読有り

    Y. Fujishima, M. Maeki, Y. Sato, T. Yasui, A. Ishida, H. Tani, Y. Baba, H. Harashima, and M. Tokeshi

    Micro Total Analysis Systems 2016     頁: 1412-1413   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  262. SIMULTANEOUS IONIC CURRENT AND OPTICAL DETECTION OF FINE PARTICULATE MATTERS (PM2.5) BASED ON CROSS-JUNCTION MICROFLUIDIC DEVICE 査読有り

    S. Rahong, T. Yasui, H. Yasaki, T. Yanagida, M. Kanai, K. Nagashima, N. Kaji, T. Kawai and Y. Baba

    Micro Total Analysis Systems 2016     頁: 1284-1285   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  263. SELF-ASSEMBLED MONOLAYER AND PEPTIDE COATED NANOWIRE DEVICES FOR SELECTIVE DETECTION OF C-REACTIVE PROTEIN AND E.COLI WITHOUT ANTIBODY 査読有り

    A. Yokoyama, T. Yasui, T. Goda, T. Yanagida, M. Tanaka, M. Muto, M. Okochi, N. Kaji, M. Kanai, K. Nagashima, Y. Miyahara, T. Kawai and Y. Baba

    Micro Total Analysis Systems 2016     頁: 816-817   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  264. RECOGNITION MICROPORES FOR DETECTING SINGLE-BACTERIA 査読有り

    M. Tsutsui, K. Yokota, T. Yasui, H. Yasaki, M. Okochi, M. Taniguchi, T. Washio, Y. Baba, and T. Kawai

    Micro Total Analysis Systems 2016     頁: 679-680   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  265. MASSIVELY PARALLEL SINGLE NUCLEI ASSAY ON MICROCHAMBER ARRAY DEVICES 査読有り

    S. Ito, N. Kaji, T. Yasui, Y. Baba

    Micro Total Analysis Systems 2016     頁: 651-652   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  266. IMMUNO-WALL LAB-ON-A-CHIP PROTEIN ANALYSIS DEVICES FOR HIGH PRECISION SURGERY OF GLIOMAS 査読有り

    T. Kasama, , A. Yamamichi, F. Ohka, Y. Kato, H. Suzuki, A. Kato, K. Motomura, M. Hirano, M. Ranjit, L. Chalise, M. Kurimoto, G. Kondo, K. Aoki, N. Kaji, T. Matsubara, H. Suzuki, M. Tokeshi, T. Wakabayashi, A. Natsume, and Y. Baba

    Micro Total Analysis Systems 2016     頁: pp.651-652   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  267. A HIGH-SENSITIVITY CYCLODEXTRIN-MICROCHIP ELECTROPHORESIS SIMULTANEOUS ANALYSIS OF GABAPENTIN AND PREGABALIN 査読有り

    A.M. Zeid, J. Nasr, N. Kaji, F.F. Belal, M.I. Walash, Y. Baba

    Micro Total Analysis Systems 2016     頁: 1457-1458   2016年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  268. SELF-ASSEMBLED MONOLAYER AND PEPTIDE COATED NANOWIRE DEVICES FOR SELECTIVE DETECTION OF C-REACTIVE PROTEIN AND E.COLI WITHOUT ANTIBODY 査読有り

    A. Yokoyama, T. Yasui, T. Goda, T. Yanagida, M. Tanaka, M. Muto, M. Okochi, N. Kaji, M. Kanai, K. Nagashima, Y. Miyahara, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2016     頁: 816-817   2016年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  269. RECOGNITION MICROPORES FOR DETECTING SINGLE-BACTERIA 査読有り

    M. Tsutsui, K. Yokota, T. Yasui, H. Yasaki, M. Okochi, M. Taniguchi, T. Washio, Y. Baba, T. Kawai

    Micro Total Analysis Systems 2016     頁: 679-680   2016年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  270. NANOWIRES FOR EARLY CANCER AND DIABETES DIAGNOSIS VIA MICRO-RNA DETECTION IN URINE EXTRACELLULAR VESICLES 査読有り

    T. Yasui, D. Takeshita, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, H. Yukawa, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2016     頁: 108-109   2016年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  271. MILLISECOND MEASUREMENT OF SINGLE CELL SIZE AND DEFORMABILITY BY ELECTRICAL DETECTION IN MICROFLUIDIC DEVICE WITH TWO CONSECUTIVE CONSTRICTIONS 査読有り

    N. Kaji, M. Sano, H. Yasaki, T. Yasui, Y. Baba

    Micro Total Analysis Systems 2016     頁: 341-342   2016年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  272. MICROFLUIDIC LONG-PORE-CHANNEL TO HIGHLIGHT BACTERIA CONTENTS 査読有り

    H. Yasaki, T. Yasui, T. Yanagida, N. Kaji, M. Kanai, M. Fukuyama, K. Nagashima, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2016     頁: 100-101   2016年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  273. MASSIVELY PARALLEL SINGLE NUCLEI ASSAY ON MICROCHAMBER ARRAY DEVICES 査読有り

    S. Ito, N. Kaji, T. Yasui, Y. Baba

    Micro Total Analysis Systems 2016     頁: 651-652   2016年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  274. IMMUNO-WALL LAB-ON-A-CHIP PROTEIN ANALYSIS DEVICES FOR HIGH PRECISION SURGERY OF GLIOMAS 査読有り

    T. Kasama, A. Yamamichi, F. Ohka, Y. Kato, H. Suzuki, A. Kato, K. Motomura, M. Hirano, M. Ranjit, L. Chalise, M. Kurimoto, G. Kondo, K. Aoki, N. Kaji, T. Matsubara, H. Suzuki, M. Tokeshi, T. Wakabayashi, A. Natsume, Y. Baba

    Micro Total Analysis Systems 2016     頁: pp.651-652   2016年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  275. EFFECTS OF THE GROOVED STRUCTURES AND THE ETHANOL CONCENTRATION ON THE SMALL-SIZED LIPID NANOPARTICLES FORMATION 査読有り

    Y. Fujishima, M. Maeki, Y. Sato, T. Yasui, A. Ishida, H. Tani, Y. Baba, H. Harashima, M. Tokeshi

    Micro Total Analysis Systems 2016     頁: 1412-1413   2016年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  276. SIMULTANEOUS IONIC CURRENT AND OPTICAL DETECTION OF FINE PARTICULATE MATTERS (PM2.5) BASED ON CROSS-JUNCTION MICROFLUIDIC DEVICE 査読有り

    S. Rahong, T. Yasui, H. Yasaki, T. Yanagida, M. Kanai, K. Nagashima, N. Kaji, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2016     頁: 1284-1285   2016年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  277. WATER FILM DEVICE FOR CONTINUOUS PARTICULATE MATTER COLLECTION 査読有り

    T. Shimada, T. Yasui, A. Hibara, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2016     頁: 1473-1474   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  278. An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas 査読有り

    Yamamichi, Akane; Kasama, Toshihiro; Ohka, Fumiharu; Suzuki, Hiromichi; Kato, Akira; Motomura, Kazuya; Hirano, Masaki; Ranjit, Melissa; Chalise, Lushun; Kurimoto, Michihiro; Kondo, Goro; Aoki, Kosuke; Kaji, Noritada; Tokeshi, Manabu; Matsubara, Toshio; Senga, Takeshi; Kaneko, Mika. K; Suzuki, Hidenori; Hara, Masahito; Wakabayashi, Toshihiko; Baba, Yoshinobu; Kato, Yukinari; Natsume, Atsushi

    Science and Technology of Advanced Materials   17 巻 ( 1 ) 頁: pp.618-625   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  279. 量子ドットによる移植幹細胞の生体内イメージング 査読有り

    湯川 博, 馬場嘉信

    光学   45 巻 ( 10 ) 頁: pp.386-391   2016年10月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  280. 量子ドットによる移植幹細胞の生体内イメージング (再生医療を推進するフォトニクス)

    湯川 博, 馬場 嘉信

    光学 = Japanese journal of optics : publication of the Optical Society of Japan   45 巻 ( 10 ) 頁: 386 - 391   2016年10月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:日本光学会  

    CiNii Article

    CiNii Books

  281. Measurement of DNA length changes upon CpG hypermethylation by microfluidic molecular stretching 査読有り

    Onoshima D*., Kawakita N., Takeshita D., Niioka H., Yukawa H., Miyake J., Baba Y.

    Cell Medicine   91 巻 ( 6 ) 頁: pp.61-66   2016年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  282. Measurement of DNA length changes upon CpG hypermethylation by microfluidic molecular stretching 査読有り

    Onoshima D, Kawakita N, Takeshita D, Niioka H, Yukawa H, Miyake J, Baba Y

    Cell Medicine   91 巻 ( 6 ) 頁: pp.61-66   2016年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  283. Multispectral Emissions of Lanthanide-Doped Gadolinium Oxide Nanophosphors for Cathodoluminescence and Near-Infrared Upconversion/Downconversion Imaging. 査読有り 国際誌

    Thi Kim Dung D, Fukushima S, Furukawa T, Niioka H, Sannomiya T, Kobayashi K, Yukawa H, Baba Y, Hashimoto M, Miyake J

    Nanomaterials (Basel, Switzerland)   6 巻 ( 9 ) 頁: 163   2016年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nanomaterials  

    Comprehensive imaging of a biological individual can be achieved by utilizing the variation in spatial resolution, the scale of cathodoluminescence (CL), and near-infrared (NIR), as favored by imaging probe Gd2O3 co-doped lanthanide nanophosphors (NPPs). A series of Gd2O3:Ln3+/Yb3+ (Ln3+: Tm3+, Ho3+, Er3+) NPPs with multispectral emission are prepared by the sol-gel method. The NPPs show a wide range of emissions spanning from the visible to the NIR region under 980 nm excitation. The dependence of the upconverting (UC)/downconverting (DC) emission intensity on the dopant ratio is investigated. The optimum ratios of dopants obtained for emissions in the NIR regions at 810 nm, 1200 nm, and 1530 nm are applied to produce nanoparticles by the homogeneous precipitation (HP) method. The nanoparticles produced from the HP method are used to investigate the dual NIR and CL imaging modalities. The results indicate the possibility of using Gd2O3 co-doped Ln3+/Yb3+ (Ln3+: Tm3+, Ho3+, Er3+) in correlation with NIR and CL imaging. The use of Gd2O3 promises an extension of the object dimension to the whole-body level by employing magnetic resonance imaging (MRI).

    DOI: 10.3390/nano6090163

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    PubMed

  284. Anomalous Separation of Small Y-Chromosomal DNA Fragments on Microchip Electrophoresis. 査読有り

    Jabasini M, Ewis A, Sato Y, Nakahori Y, Baba Y

    Scientia pharmaceutica   84 巻 ( 3 ) 頁: 507 - 513   2016年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Scientia Pharmaceutica  

    We investigated an anomalous DNA separation where two DNA fragments from the human Y-chromosome sY638 (64 bp) and sY592 (65 bp), with only one base pair difference, were separated. This result is abnormal since in a previous study, we found that 5 bp was the minimum difference between two DNA fragments that the microchip electrophoresis system can separate. The formation of a mini-loop in the structure of the DNA fragment of sY638 (64 bp) was strongly expected to be the reason. To investigate this, we synthesized three modified DNA fragments for sY638 (64 bp), and the modifications were in two expected locations for possible mini-loop formation. Later, the separation between sY592 (65 bp) and the three modified fragments of sY638 (64 bp) was not possible. Thus, we conclude that the formation of a mini-loop in the structure of the DNA is the reason behind this anomalous separation.

    DOI: 10.3390/scipharm84030507

    Web of Science

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    PubMed

  285. セミの翅の物理的防御機構を模倣したナノワイヤ構造体の創製 査読有り

    安井隆雄, 米勢明弘, 加地範匡, 柳田剛, 川合知二, 馬場嘉信

    昆虫と自然   51 巻 ( 9 ) 頁: pp.39-41   2016年8月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  286. Label-free detection of real-time DNA amplification using a nanofluidic diffraction grating. 査読有り 国際誌

    Yasui T, Ogawa K, Kaji N, Nilsson M, Ajiri T, Tokeshi M, Horiike Y, Baba Y

    Scientific reports   6 巻   頁: 31642 - 31642   2016年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Scientific Reports  

    Quantitative DNA amplification using fluorescence labeling has played an important role in the recent, rapid progress of basic medical and molecular biological research. Here we report a label-free detection of real-time DNA amplification using a nanofluidic diffraction grating. Our detection system observed intensity changes during DNA amplification of diffracted light derived from the passage of a laser beam through nanochannels embedded in a microchannel. Numerical simulations revealed that the diffracted light intensity change in the nanofluidic diffraction grating was attributed to the change of refractive index. We showed the first case reported to date for label-free detection of real-time DNA amplification, such as specific DNA sequences from tubercle bacilli (TB) and human papillomavirus (HPV). Since our developed system allows quantification of the initial concentration of amplified DNA molecules ranging from 1 fM to 1 pM, we expect that it will offer a new strategy for developing fundamental techniques of medical applications.

    DOI: 10.1038/srep31642

    Web of Science

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    PubMed

  287. INSECTS REPORTS セミの翅の物理的防御機構を模倣したナノワイヤ構造体の創製

    安井 隆雄, 米勢 明弘, 加地 範匡, 柳田 剛, 川合 知二, 馬場 嘉信

    昆虫と自然   51 巻 ( 9 ) 頁: 39 - 41   2016年8月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ニューサイエンス社  

    CiNii Article

    CiNii Books

  288. Application of a cell microarray chip system for accurate, highly sensitive, and rapid diagnosis for malaria in Uganda. 査読有り 国際誌

    Yatsushiro S, Yamamoto T, Yamamura S, Abe K, Obana E, Nogami T, Hayashi T, Sesei T, Oka H, Okello-Onen J, Odongo-Aginya EI, Alai MA, Olia A, Anywar D, Sakurai M, Palacpac NM, Mita T, Horii T, Baba Y, Kataoka M

    Scientific reports   6 巻   頁: 30136 - 30136   2016年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Scientific Reports  

    Accurate, sensitive, rapid, and easy operative diagnosis is necessary to prevent the spread of malaria. A cell microarray chip system including a push column for the recovery of erythrocytes and a fluorescence detector was employed for malaria diagnosis in Uganda. The chip with 20,944 microchambers (105 μm width and 50 μm depth) was made of polystyrene. For the analysis, 6 μl of whole blood was employed, and leukocytes were practically removed by filtration through SiO 2 -nano-fibers in a column. Regular formation of an erythrocyte monolayer in each microchamber was observed following dispersion of an erythrocyte suspension in a nuclear staining dye, SYTO 21, onto the chip surface and washing. About 500,000 erythrocytes were analyzed in a total of 4675 microchambers, and malaria parasite-infected erythrocytes could be detected in 5 min by using the fluorescence detector. The percentage of infected erythrocytes in each of 41 patients was determined. Accurate and quantitative detection of the parasites could be performed. A good correlation between examinations via optical microscopy and by our chip system was demonstrated over the parasitemia range of 0.0039-2.3438% by linear regression analysis (R 2 =0.9945). Thus, we showed the potential of this chip system for the diagnosis of malaria.

    DOI: 10.1038/srep30136

    Web of Science

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  289. The immunosuppressive effect via HGF-CMET pathway of autologous adipose tissue-derived mesenchymal stem cells in a rat lung transplantation model

    Watanabe Hironosuke, Tsuchiya Tomoshi, Yamasaki Naoya, Miyazaki Takuro, Matsumoto Keitaro, Yukawa Hiroshi, Baba Yoshinobu, Nagayasu Takeshi

    TRANSPLANTATION   100 巻 ( 7 ) 頁: S107-S108   2016年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  290. The immunosuppressive effect via HGF-CMET pathway of autologous adipose tissue-derived mesenchymal stem cells in a rat lung transplantation model 査読有り

    Watanabe Hironosuke, Tsuchiya Tomoshi, Yamasaki Naoya, Miyazaki Takuro, Matsumoto Keitaro, Yukawa Hiroshi, Baba Yoshinobu, Nagayasu Takeshi

    TRANSPLANTATION   100 巻 ( 7 ) 頁: S107 - S108   2016年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  291. Microfluidic transport through microsized holes treated by non-equilibrium atmospheric-pressure plasma 査読有り

    T. Ito, K. Ishikawa, D. Onoshima, N. Kihara, K. Tatsukoshi, H. Odaka, H. Hashizume, H. Tanaka, H. Yukawa, K. Takeda, H. Kondo, M. Sekine, Y. Baba, and M. Hori

    IEEE Trans. Plasma Sci   44 巻   頁: pp.3060-3065   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  292. Elucidation of the physicochemical properties and potency of siRNA-loaded small-sized lipid nanoparticles for siRNA delivery 査読有り

    Yusuke Sato, Yusuke Note, Masatoshi Maeki, Noritada Kaji, Yoshinobu Baba, Manabu Tokeshi, Hideyoshi Harashima

    Journal of Controlled Release   229 巻   頁: 48-57   2016年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  293. Three-dimensional fabrication for microfluidics by conventional techniques and equipment used in mass production 査読有り

    Toyohiro Naito, Makoto Nakamura, Noritada Kaji, Takuya Kubo, Yoshinobu Baba, Koji Otsuka

    Micromachines   7 巻 ( 5 ) 頁: 82   2016年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/mi7050082

  294. Correlative near-infrared light and cathodoluminescence microscopy using Y<inf>2</inf>O<inf>3</inf>:Ln, Yb (Ln = Tm, Er) nanophosphors for multiscale, multicolour bioimaging 査読有り

    Fukushima S., Furukawa T., Niioka H., Ichimiya M., Sannomiya T., Tanaka N., Onoshima D., Yukawa H., Baba Y., Ashida M., Miyake J., Araki T., Hashimoto M.

    Scientific Reports   6 巻   2016年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Scientific Reports  

    This paper presents a new correlative bioimaging technique using Y2O3:Tm, Yb and Y2O3:Er, Yb nanophosphors (NPs) as imaging probes that emit luminescence excited by both near-infrared (NIR) light and an electron beam. Under 980 nm NIR light irradiation, the Y2O3:Tm, Yb and Y2O3:Er, Yb NPs emitted NIR luminescence (NIRL) around 810 nm and 1530 nm, respectively, and cathodoluminescence at 455 nm and 660 nm under excitation of accelerated electrons, respectively. Multimodalities of the NPs were confirmed in correlative NIRL/CL imaging and their locations were visualized at the same observation area in both NIRL and CL images. Using CL microscopy, the NPs were visualized at the single-particle level and with multicolour. Multiscale NIRL/CL bioimaging was demonstrated through in vivo and in vitro NIRL deep-tissue observations, cellular NIRL imaging, and high-spatial resolution CL imaging of the NPs inside cells. The location of a cell sheet transplanted onto the back muscle fascia of a hairy rat was visualized through NIRL imaging of the Y2O3:Er, Yb NPs. Accurate positions of cells through the thickness (1.5 mm) of a tissue phantom were detected by NIRL from the Y2O3:Tm, Yb NPs. Further, locations of the two types of NPs inside cells were observed using CL microscopy.

    DOI: 10.1038/srep25950

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  295. Elucidation of the physicochemical properties and potency of siRNA-loaded small-sized lipid nanoparticles for siRNA delivery. 査読有り 国際誌

    Sato Y, Note Y, Maeki M, Kaji N, Baba Y, Tokeshi M, Harashima H

    Journal of controlled release : official journal of the Controlled Release Society   229 巻   頁: 48 - 57   2016年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Journal of Controlled Release  

    Because nanoparticles with diameters less than 50 nm penetrate stromal-rich tumor tissues more efficiently, the synthesis of small-sized nanoparticles encapsulating short interfering RNA (siRNA) is important in terms of realizing novel siRNA medicine for the treatment of various cancers. Lipid nanoparticles (LNPs) are the leading systems for the delivery of siRNA in vivo. Limit size LNPs were successfully synthesized using a microfluidic mixing technique. However, the physicochemical properties and potential for in vivo siRNA delivery of the limit-size LNPs have not been examined in detail. In the present study, we prepared LNPs with different diameters from 32 to 67 nm using a microfluidic mixing devise and examined the physicochemical properties of the particles and the potential for their use in delivering siRNA in vitro and in vivo to liver. Reducing the size of the LNPs causes poor-packing and an increased surface area, which result in their instability in serum. Moreover, it was revealed that the ability of endosomal escape (cytosolic siRNA release) of the smaller LNPs is subject to inhibition by serum compared to that of larger counterparts. Taken together, an increase in packing and avoiding the adsorption of serum components are key strategies for the development of next-generation highly potent and small-sized LNPs.

    DOI: 10.1016/j.jconrel.2016.03.019

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  296. Recent developments of nanowires for bio-applications from molecular to cellular levels 査読有り

    S. Rahong, T. Yasui, N. Kaji, Y. Baba

    Lab on a Chip   16 巻   頁: 1126-1138   2016年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  297. Recent developments in nanowires for bio-applications from molecular to cellular levels. 査読有り 国際誌

    Rahong S, Yasui T, Kaji N, Baba Y

    Lab on a chip   16 巻 ( 7 ) 頁: 1126 - 1138   2016年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Lab on a Chip  

    This review highlights the most promising applications of nanowires for bioanalytical chemistry and medical diagnostics. The materials discussed here are metal oxide and Si semiconductors, which are integrated with various microfluidic systems. Nanowire structures offer desirable advantages such as a very small diameter size with a high aspect ratio and a high surface-to-volume ratio without grain boundaries; consequently, nanowires are promising tools to study biological systems. This review starts with the integration of nanowire structures into microfluidic systems, followed by the discussion of the advantages of nanowire structures in the separation, manipulation and purification of biomolecules (DNA, RNA and proteins). Next, some representative nanowire devices are introduced for biosensors from molecular to cellular levels based on electrical and optical approaches. Finally, we conclude the review by highlighting some bio-applications for nanowires and presenting the next challenges that must be overcome to improve the capabilities of nanowire structures for biological and medical systems.

    DOI: 10.1039/c5lc01306b

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  298. Crystal Phase-Controlled Synthesis of Rod-Shaped AgInTe2 Nanocrystals for in vivo Imaging in the Near-Infrared Wavelength Region 査読有り

    Kameyama, Tatsuya; Ishigami, Yujiro; Yukawa, Hiroshi; Shimada, Taisuke; Baba, Yoshinobu; Ishikawa, Tetsuya; Kuwabata, Susumu; Torimoto, Tsukasa

    Nanoscale   8 巻   頁: 5435-5440   2016年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  299. Functional Double-Shelled Silicon Nanocrystals for Two-Photon Fluorescence Cell Imaging: Spectral Evolution and Tuning 査読有り

    Sourov Chandra, Batu Ghosh, Grory Beaune, Usharani Nagarajan, Takao Yasui, Jin Nakamura, Tohru Tsuruoka, Yoshinobu Baba, Naoto Shirahata, Francoise M. Winnik

    Nanoscale   8 巻   頁: 9009-9019   2016年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/C6NR01437B

  300. Determination of six anti-Parkinson drugs using cyclodextrin-capillary electrophoresis method: application to pharmaceutical dosage forms 査読有り

    Abdallah M. Zeid*ab, Jenny Jeehan M. Nasrb, Fathalla F. Belalb, Shinya Kitagawac, Noritada Kajiade, Yoshinobu Babaadfg and Mohamed I. Walashb

    RSC Advances   6 巻 ( 21 ) 頁: pp.17519-17530   2016年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 17519-17530

  301. 移植幹細胞in vivo蛍光イメージングが再生医療創薬に果たす役割 査読有り

    湯川博、馬場嘉信

    DDS学会誌   31 巻   頁: 135-145   2016年1月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  302. Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells on Concanavalin A-Induced Acute Liver Injury in Mice

    Yoshizumi Yasuma, Yukawa Hiroshi, Iwaki Ryoji, Fujinaka Sanae, Kanou Ayano, Kanou Yuki, Yamada Tatsuya, Nakagawa Shingo, Ohara Tomomi, Nakagiri Kenta, Ogihara Yusuke, Tsutsui Yoko, Hayashi Yumi, Ishigami Masatoshi, Baba Yoshinobu, Ishikawa Tetsuya

    CELL MEDICINE   9 巻 ( 1-2 ) 頁: 21-33   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3727/215517916X693159

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  303. Measurement of DNA Length Changes Upon CpG Hypermethylation by Microfluidic Molecular Stretching

    Onoshima Daisuke, Kawakita Naoko, Takeshita Daiki, Niioka Hirohiko, Yukawa Hiroshi, Miyake Jun, Baba Yoshinobu

    CELL MEDICINE   9 巻 ( 1-2 ) 頁: 61-66   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3727/215517916X693087

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  304. An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas. 査読有り

    Yamamichi A, Kasama T, Ohka F, Suzuki H, Kato A, Motomura K, Hirano M, Ranjit M, Chalise L, Kurimoto M, Kondo G, Aoki K, Kaji N, Tokeshi M, Matsubara T, Senga T, Kaneko MK, Suzuki H, Hara M, Wakabayashi T, Baba Y, Kato Y, Natsume A

    Science and technology of advanced materials   17 巻 ( 1 ) 頁: 618 - 625   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Science and Technology of Advanced Materials  

    World Health Organization grade II and III gliomas most frequently occur in the central nervous system (CNS) in adults. Gliomas are not circumscribed; tumor edges are irregular and consist of tumor cells, normal brain tissue, and hyperplastic reactive glial cells. Therefore, the tumors are not fully resectable, resulting in recurrence, malignant progression, and eventual death. Approximately 69–80% of grade II and III gliomas harbor mutations in the isocitrate dehydrogenase 1 gene (IDH1), of which 83–90% are found to be the IDH1-R132H mutation. Detection of the IDH1-R132H mutation should help in the differential diagnosis of grade II and III gliomas from other types of CNS tumors and help determine the boundary between the tumor and normal brain tissue. In this study, we established a highly sensitive antibody-based device, referred to as the immuno-wall, to detect the IDH1-R132H mutation in gliomas. The immuno-wall causes an immunoreaction in microchannels fabricated using a photo-polymerizing polymer. This microdevice enables the analysis of the IDH1 status with a small sample within 15 min with substantially high sensitivity. Our results suggested that 10% content of the IDH1-R132H mutation in a sample of 0.33 μl volume, with 500 ng protein, or from 500 cells is theoretically sufficient for the analysis. The immuno-wall device will enable the rapid and highly sensitive detection of the IDH1-R132H mutation in routine clinical practice.

    DOI: 10.1080/14686996.2016.1227222

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  305. Crystal phase-controlled synthesis of rod-shaped AgInTe2 nanocrystals for in vivo imaging in the near-infrared wavelength region. 査読有り 国際誌

    Kameyama T, Ishigami Y, Yukawa H, Shimada T, Baba Y, Ishikawa T, Kuwabata S, Torimoto T

    Nanoscale   8 巻 ( 10 ) 頁: 5435 - 5440   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nanoscale  

    Rod-shaped AgInTe2 nanocrystals (NCs) exhibiting intense near-band edge photoluminescence in the near-infrared (NIR) wavelength region, were successfully prepared by the thermal reaction of metal acetates and Te precursors in 1-dodecanethiol. Increasing the reaction temperature resulted in the formation of larger AgInTe2 NCs with crystal structures varying from hexagonal to tetragonal at reaction temperatures of 280 °C or higher. The energy gap was increased from 1.13 to 1.20 eV with a decrease in rod width from 8.3 to 5.6 nm, accompanied by a blue shift in the photoluminescence (PL) peak wavelength from 1097 to 1033 nm. The optimal PL quantum yield was approximately 18% for AgInTe2 NCs with rod widths of 5.6 nm. The applicability of AgInTe2 NCs as a NIR-emitting material for in vivo biological imaging was examined by injecting AgInTe2 NC-incorporated liposomes into the back of a C57BL/6 mouse, followed by in vivo photoluminescence imaging in the NIR region.

    DOI: 10.1039/c5nr07532g

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  306. Determination of six anti-Parkinson drugs using cyclodextrin-capillary electrophoresis method: Application to pharmaceutical dosage forms 査読有り

    Zeid A., Nasr J., Belal F., Kitagawa S., Kaji N., Baba Y., Walash M.

    RSC Advances   6 巻 ( 21 ) 頁: 17519 - 17530   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:RSC Advances  

    A novel capillary electrophoretic method was developed for the assay of two quaternary anti-Parkinson mixtures, entacapone, levodopa, carbidopa, and benserazide (mixture I), and selegiline, levodopa, carbidopa, and benserazide (mixture II), by using α-methyldopa as an internal standard. Furthermore, the method was extended for the determination of another anti-Parkinson drug, lisuride, as well as a psychoactive antihypertensive drug, α-methyldopa, without any modification of the general method. Separation and analyses of all compounds were simply achieved in an untreated fused-silica capillary tube (42.0 cm effective length and 50 μm internal diameter) within 7 minutes under an applied voltage of 20 kV. Optimum separation and analyses were obtained using 25 mM borate buffer (pH 9.5) containing 5 mM β-cyclodextrin as the background electrolyte. The apparatus was equipped with a diode array detector (DAD) to identify lisuride at 240 nm and all other drugs at 200 nm. The addition of 5 mM β-cyclodextrin to the borate buffer has a significant effect on the separation of entacapone and benserazide in mixture I, and on the separation of selegiline and benserazide in mixture II, which cannot be achieved without it. The proposed method was successfully applied to analyse the studied drugs in their multi-component and single-component pharmaceutical dosage forms. The analytical results proved the linearity (r2 ≥ 0.9997), accuracy, precision (% RSD < 2), and selectivity of the proposed capillary electrophoretic method.

    DOI: 10.1039/c5ra26473a

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  307. Design of wave-shaped-micro-channel for active transportation of C. elegans

    Baba Y., Nakajima M., Ichikawa A., Fukuda T.

    2016 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)     頁: .   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  308. Functional double-shelled silicon nanocrystals for two-photon fluorescence cell imaging: spectral evolution and tuning

    Chandra Sourov, Ghosh Batu, Beaune Gregory, Nagarajan Usharani, Yasui Takao, Nakamura Jin, Tsuruoka Tohru, Baba Yoshinobu, Shirahata Naoto, Winnik Francoise M.

    NANOSCALE   8 巻 ( 16 ) 頁: 9009-9019   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/c6nr01437b

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  309. Identifying DNA methylation in a nanochannel. 査読有り

    Sun X, Yasui T, Yanagida T, Kaji N, Rahong S, Kanai M, Nagashima K, Kawai T, Baba Y

    Science and technology of advanced materials   17 巻 ( 1 ) 頁: 644 - 649   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Science and Technology of Advanced Materials  

    DNA methylation is a stable epigenetic modification, which is well known to be involved in gene expression regulation. In general, however, analyzing DNA methylation requires rather time consuming processes (24–96 h) via DNA replication and protein modification. Here we demonstrate a methodology to analyze DNA methylation at a single DNA molecule level without any protein modifications by measuring the contracted length and relaxation time of DNA within a nanochannel. Our methodology is based on the fact that methylation makes DNA molecules stiffer, resulting in a longer contracted length and a longer relaxation time (a slower contraction rate). The present methodology offers a promising way to identify DNA methylation without any protein modification at a single DNA molecule level within 2 h.

    DOI: 10.1080/14686996.2016.1223516

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  310. 移植幹細胞<i>in vivo</i>蛍光イメージングが再生医療創薬研究に果たす役割

    湯川 博, 馬場 嘉信

    Drug Delivery System   31 巻 ( 2 ) 頁: 135 - 145   2016年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:日本DDS学会  

    現代の医療において、生体イメージングによる画像診断技術が果たす役割は非常に大きい。本稿では、最初に筆者らが検討を進めてきた量子ドットによる移植幹細胞in vivo蛍光イメージング技術について紹介した。そして、移植幹細胞in vivo蛍光イメージング技術が、現在、オールジャパン体制で進められている各組織・臓器、およびがん・免疫領域における再生医療の臨床研究、ひいては再生医療創薬に果たす役割について概説した。今後、最先端ナノテクノロジーによるin vivo蛍光イメージング技術と再生医療技術の融合により、再生医療創薬が早期に実現されることが期待される。(著者抄録)

    DOI: 10.2745/dds.31.135

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  311. Design of wave-shaped-micro-channel for active transportation of C. elegans

    Baba Y, Nakajima M, Ichikawa A, Fukuda T

    2016 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)     頁: .   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  312. Micro sand timer in glass membrane device separates single circulating tumor cells in blood 査読有り

    Kuboyama D., Onoshima D., Yukawa H., Tanaka M., Ishikawa K., Hori M., Baba Y.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016     頁: 297 - 298   2016年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016  

    We developed a size-exclusive micro sand timer in which a single cancer cell was trapped at the narrow neck through a trickle of blood from the upper bulb to the lower one. The cells in the bulbs could be directly subjected to enumeration and staining assays for identification of cancer cells in blood. A cell population with tumor markers was successfully detected as distinguished from other blood cells.

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  313. Multifunctional quantum dots-based cancer diagnostics and stem cell therapeutics for regenerative medicine 査読有り

    Daisuke Onoshima, Hiroshi Yukawa, Yoshinobu Baba

    Advanced Drug Delivery Reviews   95 巻   頁: 2-14   2015年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  314. Multifunctional nanobiodevices in medical sciences. 査読有り 国際誌

    Onoshima D, Yukawa H, Baba Y

    Advanced drug delivery reviews   95 巻 ( 1 ) 頁: 1 - 1   2015年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.addr.2015.11.006

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  315. Multifunctional quantum dots-based cancer diagnostics and stem cell therapeutics for regenerative medicine. 査読有り 国際誌

    Onoshima D, Yukawa H, Baba Y

    Advanced drug delivery reviews   95 巻   頁: 2 - 14   2015年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Advanced Drug Delivery Reviews  

    A field of recent diagnostics and therapeutics has been advanced with quantum dots (QDs). QDs have developed into new formats of biomolecular sensing to push the limits of detection in biology and medicine. QDs can be also utilized as bio-probes or labels for biological imaging of living cells and tissues. More recently, QDs has been demonstrated to construct a multifunctional nanoplatform, where the QDs serve not only as an imaging agent, but also a nanoscaffold for diagnostic and therapeutic modalities. This review highlights the promising applications of multi-functionalized QDs as advanced nanosensors for diagnosing cancer and as innovative fluorescence probes for in vitro or in vivo stem cell imaging in regenerative medicine.

    DOI: 10.1016/j.addr.2015.08.004

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  316. Micropillars fabricated on poly(methyl methacrylate) substrates for separation of microscale objects 査読有り

    Takao YASUI, Satoru ITO, Noritada KAJI, Manabu TOKESHI, and Yoshinobu BABA

    Analytical Sciences   31 巻 ( 11 ) 頁: 1197-1200   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2116/analsci31.1197

  317. マイクロ化学チップを用いた単一DNA分子シークエンシング法

    加地範匡、馬場嘉信

    クリーンテクノロジー   11 巻   頁: 5-8   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  318. マイクロ化学チップを用いた単一DNA分子シークエンシング法

    加地範匡, 馬場嘉信

    クリーンテクノロジー   11 巻   頁: 5-8   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  319. IMMUNOSUPPRESSIVE EFFECT OF AUTOLOGOUS ADIPOSE-TISSUE DERIVED MESENCHYMAL STEM CELL IN A RAT LUNG TRANSPLANTATION MODEL

    Watanabe Hironosuke, Tsuchiya Tomoshi, Miyazaki Takuro, Matsumoto Keitaro, Yamasaki Naoya, Yukawa Hiroshi, Baba Yoshinobu, Nagayasu Takeshi

    TRANSPLANT INTERNATIONAL   28 巻   頁: 164-164   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  320. Micropillars Fabricated on Poly(methyl methacrylate) Substrates for Separation of Microscale Objects. 査読有り

    Yasui T, Ito S, Kaji N, Tokeshi M, Baba Y

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   31 巻 ( 11 ) 頁: 1197 - 1200   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Sciences  

    Development of polymeric microfluidic devices has played an important role in the recent, rapid progress of biomedical research. Here we report a fabrication method for micropillars on poly(methyl methacrylate) (PMMA) substrates for separation of microscale objects. The fabricated micropillars enable continuous separation of microparticles only by introducing fluids. The present method offers a new strategy to fabricate polymeric prototype devices for R&D work.

    DOI: 10.2116/analsci.31.1197

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  321. Micropillars Fabricated on Poly(methyl methacrylate) Substrates for Separation of Microscale Objects

    Yasui Takao, Ito Satoru, Kaji Noritada, Tokeshi Manabu, Baba Yoshinobu

    ANALYTICAL SCIENCES   31 巻 ( 11 ) 頁: 1197-1200   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  322. IMMUNOSUPPRESSIVE EFFECT OF AUTOLOGOUS ADIPOSE-TISSUE DERIVED MESENCHYMAL STEM CELL IN A RAT LUNG TRANSPLANTATION MODEL 査読有り

    Hironosuke Watanabe, Tomoshi Tsuchiya, Takuro Miyazaki, Keitaro Matsumoto, Naoya Yamasaki, Hiroshi Yukawa, Yoshinobu Baba, Takeshi Nagayasu

    TRANSPLANT INTERNATIONAL   28 巻   頁: 164 - 164   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Web of Science

  323. NIR-II近赤外領域における移植幹細胞in vivo蛍光イメージング

    湯川 博, 小野島 大介, 新岡 宏彦, 竹内 司, 大谷 敬亨, 三宅 淳, 石川 哲也, 馬場 嘉信

    日本バイオマテリアル学会大会予稿集   37回 巻   頁: 113 - 113   2015年11月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:日本バイオマテリアル学会  

  324. Superhydrophilic glass membrane device with open-microhole array for filtering and counting rare tumor cells 査読有り

    Akihiro Yonese, Daisuke Onoshima, Hiroshi Yukawa, Kenji Ishikawa, Masaru Hori, and Yoshinobu Baba

    Micro Total Analysis Systems 2015     頁: 493-495   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  325. PDMS-anchored nanowires for high throughput micro-rna extraction from extracellular vesicles in body fluid 査読有り

    D. Takeshita, T. Yasui, H. Yong, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2015     頁: 1516-1518   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  326. A single cell nucleus array to monitor messenger-rna molecule transportation through nuclear membrane 査読有り

    N. Kaji, R. Koyama, T. Yasui, T. Higashiyama, Y. Baba

    Micro Total Analysis Systems 2015     頁: 832-834   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  327. A microchamber array device for studying genomic diversity in cancer cells at a single cell level 査読有り

    S. Ito, N. Kaji, T. Yasui, Y. Baba

    Micro Total Analysis Systems 2015     頁: 534-536   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  328. A microchamber array device for studying genomic diversity in cancer cells at a single cell level 査読有り

    S. Ito, N. Kaji, T. Yasui, Y. Baba

    Micro Total Analysis Systems 2015     頁: 534-536   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  329. PDMS-anchored nanowires for high throughput micro-rna extraction from extracellular vesicles in body fluid 査読有り

    D. Takeshita, T. Yasui, H. Yong, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2015     頁: 1516-1518   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  330. A single cell nucleus array to monitor messenger-rna molecule transportation through nuclear membrane 査読有り

    N. Kaji, R. Koyama, T. Yasui, T. Higashiyama, Y. Baba

    Micro Total Analysis Systems 2015     頁: 832-834   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  331. Microfluidic approach for production of lipid nanoparticles-based nano medicine 査読有り

    M. Maeki, T. SAITO, Y. NODE, Y. Sato, T. Yasui, N. Kaji, A. Ishida, H. Tani, Y. Baba, H. Harashima, and M. Tokeshi

    Micro Total Analysis Systems 2015     頁: 838-840   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  332. Label-free detection of extracellular vesicles for cancer diagnosis 査読有り

    Taiga Ajiri, Takao Yasui, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi

    Micro Total Analysis Systems 2015     頁: 1789-1791   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  333. Label-free detection of extracellular vesicles for cancer diagnosis 査読有り

    Taiga Ajiri, Takao Yasui, Akihiko Ishida, Hirofumi Tani, Yoshinobu Baba, Manabu Tokeshi

    Micro Total Analysis Systems 2015     頁: 1789-1791   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  334. Microfluidic approach for production of lipid nanoparticles-based nano medicine 査読有り

    M. Maeki, T. SAITO, Y. NODE, Y. Sato, T. Yasui, N. Kaji, A. Ishida, H. Tani, Y. Baba, H. Harashima, M. Tokeshi

    Micro Total Analysis Systems 2015     頁: 838-840   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  335. Cell deformability measurements for single cancer cells by ionic current in microfluidic devices 査読有り

    M. Sano, N. Kaji, T. Yasui, Y. Baba

    Micro Total Analysis Systems 2015     頁: 455-457   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  336. Nanowire devices for extracellular vesicles analysis towards elucidation of intercellular communication 査読有り

    K. Tabuchi, T. Yasui, H. Yong, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2015     頁: 1510-1512   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  337. Conformation and dynamic behavior of single DNA molecules in nanofluidic channels for detection of dna methylation 査読有り

    X. Sun, T. Yasui, S. Rahong, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2015     頁: 1115-1117   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  338. Immuno-wall lab-on-chip companion diagnostic devices for rapid and low-cost detection of mutant epidermal growth factor receptors (EGFR) from cytological samples in lung cancer patients 査読有り

    T. Kasama, T. Hase, N. Nishiwaki, N. Yogo, M. Sato, M. Kondo, N. Kaji, M. Tokeshi, Y. Hasegawa and Y. Baba

    Micro Total Analysis Systems 2015     頁: 925-927   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  339. High-throughput methylation mapping by detecting fluorescently stained methylation sites at a single molecule level 査読有り

    A. Hattori, T. Yasui, N. Kaji, Y. Baba

    Micro Total Analysis Systems 2015     頁: 861-863   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  340. Cell deformability measurements for single cancer cells by ionic current in microfluidic devices 査読有り

    M. Sano, N. Kaji, T. Yasui, Y. Baba

    Micro Total Analysis Systems 2015     頁: 455-457   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  341. Nanowire devices for extracellular vesicles analysis towards elucidation of intercellular communication 査読有り

    K. Tabuchi, T. Yasui, H. Yong, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2015     頁: 1510-1512   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  342. Immuno-wall lab-on-chip companion diagnostic devices for rapid and low-cost detection of mutant epidermal growth factor receptors (EGFR) from cytological samples in lung cancer patients 査読有り

    T. Kasama, T. Hase, N. Nishiwaki, N. Yogo, M. Sato, M. Kondo, N. Kaji, M. Tokeshi, Y. Hasegawa, Y. Baba

    Micro Total Analysis Systems 2015     頁: 925-927   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  343. High-throughput methylation mapping by detecting fluorescently stained methylation sites at a single molecule level 査読有り

    A. Hattori, T. Yasui, N. Kaji, Y. Baba

    Micro Total Analysis Systems 2015     頁: 861-863   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  344. Conformation and dynamic behavior of single DNA molecules in nanofluidic channels for detection of dna methylation 査読有り

    X. Sun, T. Yasui, S. Rahong, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2015     頁: 1115-1117   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  345. Nanowire lysis and dna extracrtion from a single bacterium for bacteria analysis 査読有り

    T. Yasui, K. Otsuka, M. Takeuchi1, T. Yanagida, N. Kaji, M. Kanai, S. Rahong, K. Nagashima, T. Naito, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2015     頁: 320-322   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  346. Highly sensitive ionic current sensing system with optical observation for discriminating a wide diversity of sizes of bacteria with contaminants 査読有り

    H. Yasaki, T. Yasui, S. Rahong, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2015     頁: 314-316   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  347. Highly sensitive ionic current sensing system with optical observation for discriminating a wide diversity of sizes of bacteria with contaminants 査読有り

    H. Yasaki, T. Yasui, S. Rahong, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2015     頁: 314-316   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  348. Nanowire lysis and dna extracrtion from a single bacterium for bacteria analysis 査読有り

    T. Yasui, K. Otsuka, M. Takeuchi, T. Yanagida, N. Kaji, M. Kanai, S. Rahong, K. Nagashima, T. Naito, T. Kawai, Y. Baba

    Micro Total Analysis Systems 2015     頁: 320-322   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  349. Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture 査読有り

    Naito Toyohito, Kaji Noritada, Tokeshi Manabu, Kubo Takuya, Baba Yoshinobu, Otsuka Koji

    ANALYTICAL METHODS   7 巻 ( 17 ) 頁: 7264 - 7269   2015年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Methods  

    Cell collection based on deterministic lateral displacement (DLD) and cell circulation with a loop channel are two component technologies for stressless cell retention which have been developed with a view to working toward suspension culture in a microfluidic channel. DLD devices with low array angles collect floating S. cerevisiae through an array effectively. The DLD device with an array angle of 2.6°showed an efficiency of 91.7%. Two types of loop channels with a piezoelectrie pump make a stable two-phase laminar flow of a pre-filled fluid and supplied fluid. A loop channel with a connection between an inlet and outlet on the opposite side replaces a filled fluid in the channel with a supplied fluid, which is essential for supplying nutrient rich medium to cells in microfluidic channels as well as retaining cells in a microenvironment without external stresses.

    DOI: 10.1039/c5ay00485c

    Web of Science

    Scopus

  350. Development of Microfluidic Devices for Rapid, Low-Cost Detection of EGFR Mutations in Cytological Samples from Patients with Lung Cancer

    Hase Tetsunari, Kasama Toshihiro, Nishiwaki Nanako, Yogo Naoyuki, Sato Mitsuo, Kaji Noritada, Kondo Masashi, Tokeshi Manabu, Baba Yoshinobu, Hasegawa Yoshinori

    JOURNAL OF THORACIC ONCOLOGY   10 巻 ( 9 ) 頁: S585-S585   2015年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  351. Fluorescence Quenching of CdSe/ZnS Quantum Dots by Using Black Hole Quencher Molecules Intermediated With Peptide for Biosensing Application 査読有り

    Sreenadh Sasidharan Pillai, Hiroshi Yukawa, Daisuke Onoshima,Vasudevanpillai Biju, and Yoshinobu Baba

    Cell Medicine   8 巻   頁: 57-62   2015年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3727/215517915X689074

  352. ナノバイオデバイスが拓く次世代医療・創薬 査読有り

    馬場嘉信

    ファルマシア   51 巻   頁: 747-749   2015年8月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  353. ナノバイオデバイスによる単一細胞解析と単一生体分子解析 査読有り

    安井隆雄,馬場嘉信

    分析化学 特集号「ミクロで拓く分析化学」   64 巻 ( 6 ) 頁: 413-419   2015年7月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  354. The Scope of Analytical Chemistry 査読有り

    Jonathan V. Sweedler, Daniel W. Armstrong, Yoshinobu Baba, Gert Desmet, Norman Dovichi, Andrew Ewing, Catherine C. Fenselau, Robert T. Kennedy, Cynthia K. Larive, Frances S. Ligler, Richard L. McCreery, Reinhard Niessner, Jeanne E. Pemberton, Weihong Tan, David R. Walt, John R. YatesIII, Renato Zenobi, and Xinrong Zhang

    Analytical Chemistry   87 巻 ( 13 ) 頁: 6425-6425   2015年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  355. ナノバイオデバイスによる単一細胞解析と単一生体分子解析 査読有り

    安井隆雄, 馬場嘉信

    分析化学 特集号「ミクロで拓く分析化学」   64 巻 ( 6 ) 頁: 413-419   2015年7月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  356. The Scope of Analytical Chemistry. 査読有り

    Sweedler JV, Armstrong DW, Baba Y, Desmet G, Dovichi N, Ewing A, Fenselau CC, Kennedy RT, Larive CK, Ligler FS, McCreery RL, Niessner R, Pemberton JE, Tan W, Walt DR, Yates JR 3rd, Zenobi R, Zhang X

    Analytical chemistry   87 巻 ( 13 ) 頁: 6425 - 6425   2015年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acs.analchem.5b02231

    Web of Science

    PubMed

  357. イムノピラーデバイスの高性能化:抗体固定化担体の改良 査読有り

    西脇 奈菜子,笠間 敏博,石田 晃彦,谷 博文,馬場 嘉信,渡慶次 学

    分析化学 特集号「生(bio)」   64 巻 ( 5 ) 頁: 329-335   2015年6月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  358. Nanobiodevice-based Single Cell Analysis and Single Biomolecule Analysis

    Yasui Takao, Baba Yoshinobu

    BUNSEKI KAGAKU   64 巻 ( 6 ) 頁: 413-419   2015年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  359. Three-dimensional Nanowire Structures for Ultra-Fast Separation of DNA, Protein and RNA Molecules, 査読有り

    Sakon Rahong, Takao Yasui, Takeshi Yanagida, Kazuki Nagashima, Masaki Kanai, Gang Meng, Yong He, Fuwei Zhuge, Noritada Kaji, Tomoji Kawai and Yoshinobu Baba,

    Sci. Rep.   5 巻   頁: 10584   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  360. Rapid, highly sensitive, and simultaneous detection of staphylococcal enterotoxins in milk by using immuno-pillar devices 査読有り

    Toshihiro Kasama, Mai Ikami, Wanchun Jin, Keiko Yamada, Noritada Kaji, Yusuke Atsumi, Makoto Mizutani, Atsushi Murai, Akira Okamoto, Takao Namikawa, Michio Ohta, Manabu Tokeshi, and Yoshinobu Baba

    Analytical Methods   12 巻 ( 7 ) 頁: 5092-5095   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  361. Three-dimensional Nanowire Structures for Ultra-Fast Separation of DNA, Protein and RNA Molecules, 査読有り

    Sakon Rahong, Takao Yasui, Takeshi Yanagida, Kazuki Nagashima, Masaki Kanai, Gang Meng, Yong He, Fuwei Zhuge, Noritada Kaji, Tomoji Kawai, Yoshinobu Baba

    Sci. Rep.   5 巻   頁: 10584   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  362. A Strategy for Synthesis of Lipid Nanoparticles Using Microfluidic Devices with a Mixer Structure RSC Advances 査読有り

    M. Maeki, T. Saito, Y. Sato, T. Yasui, N. Kaji, A. Ishida, H. Tani, Y. Baba, H. Harashima, M. Tokeshi

    RSC Advances   5 巻   頁: 46181-46185   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  363. A Strategy for Synthesis of Lipid Nanoparticles Using Microfluidic Devices with a Mixer Structure RSC Advances 査読有り

    M. Maeki, T. Saito, Y. Sato, T. Yasui, N. Kaji, A. Ishida, H. Tani, Y. Baba, H. Harashima, M. Tokeshi

    RSC Advances   5 巻   頁: 46181-46185   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  364. Three-dimensional fabrication for microfluidics by conventional techniques and equipment used in mass production 査読有り

    Toyohiro Naito *, Makoto Nakamura, Noritada Kaji, Takuya Kubo, Yoshinobu Baba, Koji Otsuka

    Micromachies   7 巻   頁: 82   2015年5月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/mi7050082

  365. Development of High-performance Immuno-pillar Devices: Improvement of Antibody-immobilized Solid Support

    Nishiwaki Nanako, Kasama Toshihiro, Ishida Akihiko, Tani Hirofumi, Baba Yoshinobu, Tokeshi Manabu

    BUNSEKI KAGAKU   64 巻 ( 5 ) 頁: 329-335   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  366. Arrangement of a nanostructure array to control equilibrium and non-equilibrium transports of macromolecules 査読有り

    Yasui Takao, Kaji Noritada, Ogawa Ryo, Hashioka Shingi, Tokeshi Manabu,Horiike Yasuhiro, Baba Yoshinobu

    Nano Lett.   15 巻   頁: 3445-3451   2015年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  367. Arrangement of a nanostructure array to control equilibrium and non-equilibrium transports of macromolecules 査読有り

    Yasui Takao, Kaji Noritada, Ogawa Ryo, Hashioka Shingi, Tokeshi Manabu, Horiike Yasuhiro, Baba Yoshinobu

    Nano Lett.   15 巻   頁: 3445-3451   2015年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  368. Self-assembled Nanowire Arrays as Three-dimensional Nanopores for Filtration of DNA Molecules 査読有り

    Sakon RAHONG, Takao YASUI, Takeshi YANAGIDA, Kazuki NAGASHIMA, Masaki KANAI, Gang MENG, Yong HE, Fuwei ZHUGE, Noritada KAJI, Tomoji KAWAI, and Yoshinobu BABA

    Anal. Sci.   31 巻 ( 3 ) 頁: 153-157   2015年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  369. 量子ドットによる移植幹細胞in vivoイメージング 査読有り

    湯川博、馬場嘉信

    分析化学 特集号「生(bio)」   64 巻 ( 2 ) 頁: 89-97   2015年3月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  370. ナノバイオデバイス創製と未来医療への展開 ナノとバイオと医療の境界領域に挑む 査読有り

    馬場嘉信

    化学と工業   68 巻 ( 3 ) 頁: 274-276   2015年3月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  371. Self-assembled Nanowire Arrays as Three-dimensional Nanopores for Filtration of DNA Molecules

    Rahong Sakon, Yasui Takao, Yanagida Takeshi, Nagashima Kazuki, Kanai Masaki, Meng Gang, He Yong, Zhuge Fuwei, Kaji Noritada, Kawai Tomoji, Baba Yoshinobu

    ANALYTICAL SCIENCES   31 巻 ( 3 ) 頁: 153-157   2015年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  372. Self-assembled Nanowire Arrays as Three-dimensional Nanopores for Filtration of DNA Molecules 査読有り

    Sakon Rahong, Takao Yasui, Takeshi Yanagida, Kazuki Nagashima, Masaki Kanai, Gang Meng, Yong He, Fuwei Zhuge, Noritada Kaji, Tomoji Kawai, Yoshinobu Baba

    ANALYTICAL SCIENCES   31 巻 ( 3 ) 頁: 153 - 157   2015年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC ANALYTICAL CHEMISTRY  

    Molecular filtration and purification play important roles for biomolecule analysis. However, it is still necessary to improve efficiency and reduce the filtration time. Here, we show self-assembled nanowire arrays as three-dimensional (3D) nanopores embedded in a microfluidic channel for ultrafast DNA filtration. The 3D nanopore structure was formed by a vapor-liquid-solid (VLS) nanowire growth technique, which allowed us to control pore size of the filtration material by varying the number of growth cycles. lambda DNA molecules (48.5 kbp) were filtrated from a mixture of T4 DNA (166 kbp) at the entrance of the 3D nanopore structure within 1 s under an applied electric field. Moreover, we observed single DNA molecule migration of T4 and lambda DNA molecules to clarify the filtration mechanism. The 3D nanopore structure has simplicity of fabrication, flexibility of pore size control and reusability for biomolecule filtration. Consequently it is an excellent material for biomolecular filtration.

    Web of Science

  373. 私の自慢 ナノバイオデバイス創製と未来医療への展開 : ナノとバイオと医療の境界領域に挑む

    馬場 嘉信

    化学と工業 = Chemistry & chemical industry   68 巻 ( 3 ) 頁: 274 - 276   2015年3月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:日本化学会  

    CiNii Article

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  374. Influence of Autofluorescence Derived From Living Body on In Vivo Fluorescence Imaging Using Quantum Dots. 国際誌

    Yukawa H, Watanabe M, Kaji N, Baba Y

    Cell medicine   7 巻 ( 2 ) 頁: 75 - 82   2015年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3727/215517914X685169

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    PubMed

  375. In vivo Imaging of Transplanted Stem Cells Using Quantum Dots

    Yukawa Hiroshi, Baba Yoshinobu

    BUNSEKI KAGAKU   64 巻 ( 2 ) 頁: 89-97   2015年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  376. 量子ドットによる移植幹細胞<i>in vivo</i>イメージング 査読有り

    湯川 博, 馬場 嘉信

    分析化学   64 巻 ( 2 ) 頁: 89 - 97   2015年2月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:公益社団法人 日本分析化学会  

    半導体材料や希土類錯体からなる量子ドットは,量子サイズ効果により従来の有機系蛍光プローブとは異なる優れた蛍光特性(超高感度,超高精細,超長寿命など)を有することから,その応用は分析化学の各分野に急速に拡大している.本論文では,近年,著者らが検討を進めてきた量子ドットの再生医療,殊に幹細胞移植治療への応用について紹介した.具体的には,ポリカチオン性リポソームや膜透過性ペプチドを用いた,半導体材料からなる量子ドットによる高効率幹細胞ラベリング技術,及び小動物用蛍光<i>in vivo</i>イメージング装置を用いた,近赤外領域に蛍光を発する量子ドットによる移植幹細胞<i>in vivo</i>イメージングについて概説した.

    DOI: 10.2116/bunsekikagaku.64.89

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  377. マイクロ抽出場によるヒト細胞由来のマイクロRNA抽出法 査読有り

    岡本 行広,日比野 理人,加地 範匡, 渡慶次 学, 馬場 嘉信

    分析化学   64 巻   頁: 9-13   2015年1月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  378. ここまで進んだがんの診断 エクソソーム・マイクロRNAによる超早期がん診断の最前線 査読有り

    湯川博,馬場嘉信

    ライフライン21 がんの先進医療   16 巻   頁: 46-48   2015年1月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  379. Microfluidic transfer of liquid interface for parallel stretching and stamping of terminal-unmodified single DNA molecules between zigzag-shaped microgrooves 査読有り

    Hirotoshi Yasaki, Daisuke Onoshima, Takao Yasui, Hiroshi Yukawa, Notitada Kaji, and Yoshinobu Baba

    Lab on a Chip   15 巻   頁: 135-140   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  380. Microfluidic transfer of liquid interface for parallel stretching and stamping of terminal-unmodified single DNA molecules between zigzag-shaped microgrooves 査読有り

    Hirotoshi Yasaki, Daisuke Onoshima, Takao Yasui, Hiroshi Yukawa, Notitada Kaji, Yoshinobu Baba

    Lab on a Chip   15 巻   頁: 135-140   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  381. Carbon nanotubes and modern nanoagriculture 査読有り

    Serag M.F., Kaji N., Tokeshi M., Baba Y.

    Nanotechnology and Plant Sciences: Nanoparticles and Their Impact on Plants     頁: 183 - 201   2015年1月

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    記述言語:日本語   掲載種別:論文集(書籍)内論文   出版者・発行元:Nanotechnology and Plant Sciences: Nanoparticles and Their Impact on Plants  

    Since their discovery, carbon nanotubes have been prominent members of the nanomaterial family. Owing to their extraordinary physical, chemical, and mechanical properties, carbon nanotubes have been proven to be a useful tool in the field of plant science. They were frequently perceived to bring about valuable biotechnological and agricultural applications that still remain beyond experimental realization. An increasing number of studies have demonstrated the ability of carbon nanotubes to traverse different plant cell barriers. These studies, also, assessed the toxicity and environmental impacts of these nanomaterials. The knowledge provided by these studies is of practical and fundamental importance for diverse applications including intracellular labeling and imaging, genetic transformation, and for enhancing our knowledge of plant cell biology. Although different types of nanoparticles have been found to activate physiological processes in plants, carbon nanotubes received particular interest. Following addition to germination medium, carbon nanotubes enhanced root growth and elongation of some plants such as onion, cucumber and rye-grass. They, also, modulated the expression of some genes that are essential for cell division and plant development. In addition, multi-walled carbon nanotubes were evidenced to penetrate thick seed coats, stimulate germination, and to enhance growth of young tomato seedlings. Multi-walled carbon nanotubes can penetrate deeply into the root system and further distribute into the leaves and the fruits. In recent studies, carbon nanotubes were reported to be chemically entrapped into the structure of plant tracheary elements. This should activate studies in the fields of plant defense and wood engineering. Although, all of these effects on plant physiology and plant developmental biology have not been fully understood, the valuable findings promises more research activity in the near future toward complete scientific understanding of the behavior of carbon nanotubes in plants. This chapter focuses on the impact of carbon nanotubes on plants and the potential use of these unique nanomaterials in crop management and plant biotechnology.

    DOI: 10.1007/978-3-319-14502-0_10

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  382. Development of a Micro RNA Extraction Chip from Human Tumor Cells

    Okamoto Yukihiro, Hibino Ayato, Kaji Noritada, Tokeshi Manabu, Baba Yoshinobu

    BUNSEKI KAGAKU   64 巻 ( 1 ) 頁: 9-13   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  383. Nanobiodevices for single biomolecular separations

    Y. Baba

    Chromatography   36 巻   頁: 73-79   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  384. Influence of Autofluorescence Derived From Living Body on In Vivo Fluorescence Imaging Using Quantum Dots 査読有り

    Yukawa, Hiroshi; Watanabe, Masaki; Kaji, Noritada; Baba, Yoshinobu

    Cell Medicine   7 巻 ( 2 ) 頁: 75-82   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  385. Multifunctional nanobiodevices in Medical Sciences 査読有り

    D. Onoshima, H. Yukawa, Y. Baba

    Adv. Drug Del. Rev.   95 巻 ( 1 ) 頁: 00   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  386. マイクロ抽出場によるヒト腫瘍細胞由来のマイクロRNA抽出法

    岡本 行広, 日比野 理人, 加地 範匡, 渡慶次 学, 馬場 嘉信

    分析化学   64 巻 ( 1 ) 頁: 9 - 13   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:日本分析化学会  

    The importance of micro RNA analysis has been significantly increasing because the role of micro RNA in the human body has been gradually revealed. Despite its importance, the analysis has suffered from several troublesome pretreatments, which hamper any easy analysis. Therefore, an easy and high-throughput pretreatment method has been demanded. In this paper, we focused on the great advantages of microchip pretreatments over conventional manual pretreatments and developed a microchip for micro RNA extraction. To simplify microchip fabrication, we adopted poly(dimethyl siloxane) (PDMS) microchip and a silica membrane, which has rolls in RNA extraction fields. With silica membranes and the adhesion nature of PDMS, we could easily fabricate RNA extraction fields in the microchip. With this microchip, we successfully extracted micro RNA from cancer tumor cells. Though this is a preliminary experiment, and still has many improvement points, our device is expected to be applied for easy and fast micro RNA extraction from biological samples.

    DOI: 10.2116/bunsekikagaku.64.9

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  387. イムノピラーデバイスの高性能化:抗体固定化担体の改良

    西脇 奈菜子, 笠間 敏博, 石田 晃彦, 谷 博文, 馬場 嘉信, 渡慶次 学

    分析化学   64 巻 ( 5 ) 頁: 329 - 335   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:日本分析化学会  

    著者らはこれまでに,光硬化性樹脂と抗体を固定化したビーズを組み合わせた,イムノアッセイによる疾病マーカー検出のためのイムノピラーデバイスと呼ばれるマイクロデバイスの開発を行ってきた.しかし,臨床診断に応用するためには,さらなる性能の向上が求められていた.そこで,イムノピラーデバイスの高性能化を目指し,抗体固定化担体の改良に取り組んだ.従来のイムノピラーデバイスはポリスチレンビーズに抗体を物理吸着により固定化してきた.本研究では,化学結合によりビーズ表面に抗体を固定化することのできるアフィニティビーズを用いたイムノピラーデバイスを作製し,C反応性タンパク質(CRP)の検出を試みた.インキュベーション時間とピラーの直径の検討を行った結果,全測定時間23分で,検出限界0.1 ng mL<sup>-1</sup>のCRPを測定することが可能となり,従来のデバイスよりも感度が100倍高いことが明らかとなった.本イムノピラーデバイスの長期保存安定性の評価を行ったところ,9か月以上保存可能であることが分かった.以上により,従来よりも優れた感度を有する,高性能なイムノアッセイ用マイクロデバイスを開発することができた.

    DOI: 10.2116/bunsekikagaku.64.329

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  388. Microfluidic transfer of liquid interface for parallel stretching and stamping of terminal-unmodified single DNA molecules in zigzag-shaped microgrooves

    Yasaki Hirotoshi, Onoshima Daisuke, Yasui Takao, Yukawa Hiroshi, Kaji Noritada, Baba Yoshinobu

    LAB ON A CHIP   15 巻 ( 1 ) 頁: 135-140   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/c4lc00990h

    Web of Science

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  389. Nano- and Microbiodevices for High-Performance Separation of Biomolecules 国際誌

    Baba Yoshinobu

    CHROMATOGRAPHY   36 巻 ( 3 ) 頁: 73-79 - 79   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:クロマトグラフィー科学会  

    Nano- and microbiodevices were developed for the high-performance separation of DNA, RNA, protein, sugar chain, and other biomolecules. We describe numerous advantages of nano- and microbiodevices as the separation technologies, including HPLC and capillary electrophoretic separation of DNA, nanopillar devices for the ultra-fast separation of DNA and proteins, nanoball materials for the fast separation of wide range of DNA fragments, nanowire devices for ultra-fast separation of DNA, RNA, and proteins. Because of the high efficiency of nano- and microbiodevices for the separation of biomolecules in the clinical blood and urea samples, numerous types of nano- and microbiodevices were developed and applied to the diagnosis of diseases, including cancer, diabetes, hypertension, infectious diseases, and other noncommunicable diseases.

    DOI: 10.15583/jpchrom.2015.030

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  390. Rapid, highly sensitive, and simultaneous detection of staphylococcal enterotoxins in milk by using immuno-pillar devices 査読有り

    Kasama Toshihiro, Ikami Mai, Jin Wanchun, Yamada Keiko, Kaji Noritada, Atsumi Yusuke, Mizutani Makoto, Murai Atsushi, Okamoto Akira, Namikawa Takao, Ohta Michio, Tokeshi Manabu, Baba Yoshinobu

    ANALYTICAL METHODS   7 巻 ( 12 ) 頁: 5092 - 5095   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Methods  

    Staphylococcal enterotoxins (SEs) have repeatedly caused food poisoning incidents worldwide. Some of the challenges associated with food poisoning outbreaks are that traditional detection methods are expensive and require long processing times and trained technicians. Microchannel devices represent a potential detection method by which these difficulties can be overcome. In this paper, we propose that immuno-pillar devices may represent a rapid, highly sensitive, and low-cost analytical system for the simultaneous detection of staphylococcal enterotoxin types A, B, and D (SEA, SEB, and SED) in milk. To prepare milk samples simulating food contaminated with SEs, commercial milk was spiked with equal amounts of SEA, SEB, and SED. A quantitative analysis of the milk samples was performed within 15 min by using the microchannel device. The analysis required only 0.5 μL of untreated milk sample. The resultant limit of detection was 15.6 pg mL<sup>-1</sup> for each SE, and the total assay time and sensitivity were markedly shorter and higher, respectively, than those for commercially available assay kits. The detection range of each enterotoxin using these devices was estimated as 15.6 pg mL<sup>-1</sup> to 100 ng mL<sup>-1</sup>, which completely covers the SE concentrations that can lead to foodborne diseases based on the U.S. Food and Drug Administration's criterion for the infectious SE dose in SE poisoning (1 μg SE). Using our devices, frequent assessment of food potentially contaminated with SE is possible.

    DOI: 10.1039/c5ay00698h

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  391. ナノバイオデバイスが拓く未来医療・創薬

    馬場 嘉信

    ファルマシア   51 巻 ( 8 ) 頁: 747 - 749   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:公益社団法人 日本薬学会  

    ナノテクノロジーは,半導体微細加工技術や自己組織化技術により,原子・分子サイズで精密にサイズを制御したナノバイオデバイスを開発することができる.私の研究室は,ナノテクノロジーを駆使して,医工薬などの異分野融合の基礎研究を展開するとともに,産学連携による医療・創薬応用可能なナノバイオデバイスの実用化を進めており,ナノポア1分子DNAシークエンシング※,単一分子計測・単一細胞計測に基づくがん超早期診断と疾患診断,がん治療・ドラッグデリバリーシステム(drug delivery system:DDS),iPS細胞再生医療用<i>in vivo</i>イメージング,がん診断・治療融合などの成果を上げてきた.<br>本稿では,私が研究を始めた動機から,どのように異分野の壁を乗り越えて,基礎研究から実用化まで研究を展開してきたのか振り返ってみたい.

    DOI: 10.14894/faruawpsj.51.8_747

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  392. ナノポア1分子DNAシークエンシング技術

    馬場 嘉信

    ファルマシア   51 巻 ( 8 ) 頁: 775_1-775_1   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    シリコン上に,DNAの直径と同程度(2nm)の孔(ナノポア)を形成し,ナノポア中にDNAが通り抜ける間隔で電極を作成したデバイスを用いて,DNAのシークエンシングを行う技術である.ナノポア中の電極間にトンネル電流を流し,1分子の1本鎖DNAを一定速度で通過させると,DNAの各塩基の電子状態の違いにより,トンネル電流値が変化するために,その変化量を解析しDNA配列を解読できる.DNA解読速度は,1秒間に1,000塩基程度であり,1,000個のナノポアを並列化することで,1時間で30億塩基のヒト・ゲノム解析が可能になる.DNAのみならず,RNA,メチル化DNA,タンパク質もシークエンシング可能である.

    DOI: 10.14894/faruawpsj.51.8_775_1

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  393. ナノピラー・ナノワイヤチップによる核酸の高速分離法

    加地 範匡, 馬場 嘉信

    電気泳動   59 巻 ( 2 ) 頁: 61-63   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2198/electroph.59.61

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  394. ナノバイオデバイスによるがん細胞由来エクソソーム解析

    湯川 博, 馬場 嘉信

    Organ Biology   22 巻 ( 2 ) 頁: 193-198   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We herein showed the analysis of exosomes derived from cancer cells by nanobiodevices. At fi rst, the nano-pore device formed by an electrode couple on a nano-pore was developed. The number and size of exosomes could be analyzed by measuring the current value of the tunnel current of exosomes in the culture supernatant poured into nano-pore device. Next, the nano-wire device formed by nano-wires on a microfl uidic device made from polymethylmethacrylate (PMMA) was developed. The exosomes could be collected from the culture supernatant with high effi ciency by using this nano-wire device, and then miRNA existing in the exosomes could be extracted by pouring the miRNA extraction liquid into the nanowire device. In addition, these devices were available to the analysis of exosomes in the serum and urine. Therefore, the analysis of exosomes derived from cancer cells by nanobiodevices may be useful for the diagnosis of cancers in early stage.

    DOI: 10.11378/organbio.22.193

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  395. Influence of Autofluorescence Derived From Living Body on In Vivo Fluorescence Imaging Using Quantum Dots 査読有り

    Yukawa, Hiroshi, Watanabe, Masaki, Kaji, Noritada, Baba, Yoshinobu

    Cell Medicine   7 巻 ( 2 ) 頁: 75-82   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  396. Nanobiodevices for single biomolecular separations

    Y. Baba

    Chromatography   36 巻   頁: 73-79   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  397. ナノバイオデバイスが拓く未来医療

    馬場嘉信

      7 巻 ( 6 ) 頁: 1-15   2014年12月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  398. Carbon nanowall scaffold to control culturing of cervical cancer cells 査読有り

    Watanabe Hitoshi, Kondo Hiroki, Okamoto Yukihiro, Hiramatsu Mineo, Sekine Makoto, Baba Yoshinobu, Hori Masaru

    APPLIED PHYSICS LETTERS   105 巻 ( 24 )   2014年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Applied Physics Letters  

    The effect of carbon nanowalls (CNWs) on the culturing rate and morphological control of cervical cancer cells (HeLa cells) was investigated. CNWs with different densities were grown using plasma-enhanced chemical vapor deposition and subjected to post-growth plasma treatment for modification of the surface terminations. Although the surface wettability of the CNWs was not significantly dependent on the CNWdensities, the cell culturing rates were significantly dependent. Morphological changes of the cells were not significantly dependent on the density of CNWs. These results indicate that plasma-induced surface morphology and chemical terminations enable nanobio applications using carbon nanomaterials.

    DOI: 10.1063/1.4902054

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  399. Synthesis and Reactions of Ynolates via a Stop-Flow Method with a Flow Microreactor 査読有り

    Toshiya Yoshiiwa, Satoshi Umezu, Manabu Tokeshi, Yoshinobu Babad and Mitsuru Shindo

    J. Flow Chem.   4 巻   頁: 180-184   2014年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  400. Synthesis and Reactions of Ynolates via a Stop-Flow Method with a Flow Microreactor 査読有り

    Toshiya Yoshiiwa, Satoshi Umezu, Manabu Tokeshi, Yoshinobu Babad, Mitsuru Shindo

    J. Flow Chem.   4 巻   頁: 180-184   2014年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  401. 超解像蛍光顕微鏡の開発

    加地範匡、渡慶次学、馬場嘉信

    現代化学   ( 12 ) 頁: 31-34   2014年12月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  402. 光学顕微鏡の限界を超える ノーベル化学賞に輝く超解像顕微鏡の世界

    馬場嘉信

    化学と工業   67 巻 ( 12 ) 頁: 1065-1066   2014年12月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  403. 2014年ノーベル化学賞 超解像蛍光顕微鏡の開発

    加地 範匡, 渡慶次 学, 馬場 嘉信

    現代化学   ( 525 ) 頁: 31 - 34   2014年12月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:東京化学同人  

    CiNii Article

    CiNii Books

  404. 光学顕微鏡の限界を超える ノーベル化学賞に輝く超解像顕微鏡の世界

    馬場嘉信

    化学と工業   67 巻 ( 12 ) 頁: 1065-1066   2014年12月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  405. Nanopillar, Nanowall, and Nanowire Devices for Fast Separation of Biomolecules

    Yasui Takao, Rahong Sakon, Kaji Noritada, Baba Yoshinobu

    ISRAEL JOURNAL OF CHEMISTRY   54 巻 ( 11-12 ) 頁: 1556 - 1563   2014年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/ijch.201400102

    Web of Science

  406. ここまで進んだがんの診断・ 血中マーカーによる診断と呼気診断の最前線

    小野島大介、馬場嘉信

    ライフライン21 がんの先進医療   ( 15 ) 頁: 46-48   2014年10月

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  407. A MILLISECOND MICRO-RNA EXTRACTION TECHNIQUE FOR NANOPORE-BASED NUCLEIC ACID SEQUENCING 査読有り

    Qiong Wu, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Noritada Kaji, Manabu Tokeshi, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba

    MicroTAS 2014     頁: 233-235   2014年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  408. SIZING AND SORTING OF SINGLE DNA MOLECULES BY MICROFLUIDIC MOLECULAR COMBING DEVICE 査読有り

    Daisuke Onoshima, Naoko Kawakita, Daiki Takeshita, Hiroshi Yukawa, and Yoshinobu Baba

    MicroTAS 2014     頁: 1775-1777   2014年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  409. ADDITIVE MANUFACTURING BASED ON INJECTION MOLDING FOR THREE DIMENSIONAL MICROFLUIDICS 査読有り

    Toyohiro Naito, Makoto Nakamura, Takuya Kubo, Takao Yasui, Noritada Kaji, Yoshinobu Baba and Koji Otsuka

    MicroTAS 2014     頁: 1686-1688   2014年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  410. MICROFLUIDIC STRETCHING OF DNA WITH FLUORESCENT GOLD NANOPARTICLE FOR OPTICAL/ELECTRON MICROSCOPIC IMAGING OF A SINGLE DNA METHYLATION 査読有り

    Daiki Takeshita, Daisuke Onoshima, Yukawa Hiroshi, Takao Yashui, Noritada Kaji and Yoshinobu Baba

    MicroTAS 2014     頁: 2348-2350   2014年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  411. DEVELOPMENT OF IMMUNO-WALL DEVICES AND A MOBILE FLUORESCENCE READER FOR ON-SITE SAMPLE-TO-ANSWER IMMUNOASSAY 査読有り

    Toshihiro Kasama, Yutaka Hasegawa, Haruyasu Kondo, Tsutomu Ozawa, Noritada Kaji, Manabu Tokeshi and Yoshinobu Baba

    MicroTAS 2014     頁: 935-937   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  412. QUANTITATIVE EVALUATION OF INSULATING PERFORMANCE ON SILICON NANOBIODEVICES FOR FULLY INTEGRATED NANOPORE SINGLE DNA SEQUENCING 査読有り

    Mamiko Sano, Takao Yasui, Noritada Kaji, Masateru Taniguchi, Tomoji Kawai, Yoshinobu Baba

    MicroTAS 2014     頁: 1479-1481   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  413. MICROPORE CHANELL-BASED SIMULTANEOUS ELECTRICAL AND OPTICAL SENSING FROM SINGLE BIOMOLECULES, SINGLEEXOSOMES TO SINGLE CELLS 査読有り

    Hirotoshi Yasaki, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Noritada Kaji, Masaki Kanai, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba

    MicroTAS 2014     頁: 2161-2163   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  414. GEOMETRICAL CONTROL OF A SINGLE DNA MOLECULE TRANSLOCATION VELOCITY IN NANOPORE CHANNELS 査読有り

    Xiaoyin Sun, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Noritada Kaji, Masaki Kanai, Kazuki Nagashima, Tomoji Kawai and Yoshinobu Baba

    MicroTAS 2014     頁: 1338-1340   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  415. ULTRAFAST SEPARATION OF SMALL BIOMOLECULES BY THREE-DIMENSIONAL NANOWIRE STRUCTURE 査読有り

    Sakon Rahong, Takao Yasui, Takeshi Yanagida, Masaki Kanai, Kazuki Nagashima, Noritada Kaji, Tomoji Kawai and Yoshinobu Baba

    MicroTAS 2014     頁: 2432-2434   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  416. EXOSOMAL MEMBRANE PROTEIN DETECTION BY NANOWIRE DEVICE 査読有り

    Yuki Konakade, Takao Yasui, Takeshi Yanagida, Noritada Kaji, Yong He, Masaki Kanai, Kazuki Nagashima, Hiroshi Yukawa, Tomoji Kawai, Yoshinobu Baba

    MicroTAS 2014     頁: 2017-2019   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  417. EXOSOMAL MICRO-RNA ANALYSIS IN URINE OR SERUM USING NANOWIRE STRUCTURES 査読有り

    Takao Yasui, Satoru Ito, Takeshi Yanagida, Yong He, Sakon Rahong, Masaki Kanai, Kazuki Nagashima, Hiroshi Yukawa, Noritada Kaji, Tomoji Kawai and Yoshinobu Baba

        頁: 680-682   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  418. Nanobiodevices for Single DNA and Cell Analysis 査読有り

    N. Kaji, Y. Baba

    Micro Total Analysis Systems 2014     頁: 264-266   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  419. ADDITIVE MANUFACTURING BASED ON INJECTION MOLDING FOR THREE DIMENSIONAL MICROFLUIDICS 査読有り

    Toyohiro Naito, Makoto Nakamura, Takuya Kubo, Takao Yasui, Noritada Kaji, Yoshinobu Baba, Koji Otsuka

    MicroTAS 2014     頁: 1686-1688   2014年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  420. SIZING AND SORTING OF SINGLE DNA MOLECULES BY MICROFLUIDIC MOLECULAR COMBING DEVICE 査読有り

    Daisuke Onoshima, Naoko Kawakita, Daiki Takeshita, Hiroshi Yukawa, Yoshinobu Baba

    MicroTAS 2014     頁: 1775-1777   2014年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  421. Microfluidic transfer of liquid interface for parallel stretching and stamping of terminal-unmodified single DNA molecules between zigzag-shaped microgrooves 査読有り

    Hirotoshi Yasaki, Daisuke Onoshima, Takao Yasui, Hiroshi Yukawa, Notitada Kaji, and Yoshinobu Baba

    Lab on a Chip     頁: DOI   2014年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/C4LC00990H

  422. Nanobiodebice-based single biomolecule analysis, single-cell analysis, and in vivo imaging for cancer diagnosis, cancer theranostics, and iPS cell-based regenerative medicine 査読有り

    N. Kaji, Y. Baba

    Anal. Sci.   30 巻   頁: 859-864   2014年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  423. Nanobiodevice-based Single Biomolecule Analysis, Single-Cell Analysis, and in vivo Imaging for Cancer Diagnosis, Cancer Theranostics, and iPS Cell-based Regenerative Medicine

    Kaji Noritada, Baba Yoshinobu

    ANALYTICAL SCIENCES   30 巻 ( 9 ) 頁: 859-864   2014年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  424. ここまで進んだがんの診断・ 血中循環がん細胞の最新検出技術

    笠間敏博、馬場嘉信

    ライフライン21 がんの先進医療   14 巻   頁: 44-46   2014年7月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  425. ナノ流路を用いた単一分子解析技術 査読有り

    加地範匡、安井隆雄、馬場嘉信

    ぶんせき   ( 7 ) 頁: 348-354   2014年7月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: n/a

  426. Ultrafast and Wide Range Analysis of DNA Molecules Using Rigid Network Structure of Solid Nanowires 査読有り

    Sakon Rahong, Takao Yasui, Takeshi Yanagida, Kazuki Nagashima, Masaki Kanai, Annop Klamchuen, Gang Meng, Yong He, Fuwei Zhuge, Noritada Kaji, Tomoji Kawai & Yoshinobu Baba

    Scientific Reports   4 巻   頁: 5252   2014年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/srep05252

  427. Synthesis and Reactions of Ynolates via a Stop-Flow Method with a Flow Microreactor 査読有り

    Toshiya Yoshiiwa,Satoshi Umezu,Manabu Tokeshi,Yoshinobu Baba and Mitsuru Shindo

    J. Flow Chem.     頁: 10.1556/JFC-D-14-00008   2014年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1556/JFC-D-14-00008

  428. ナノバイオデバイスによるエクソソーム解析 査読有り

    湯川博、安井隆雄、馬場嘉信

      6 巻   頁: n/a   2014年5月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: n/a

  429. 量子ドット 査読有り

    湯川博、馬場嘉信

    再生医療 日本再生医療学会雑誌   13 巻 ( 2 ) 頁: 168-169   2014年5月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  430. がん早期診断の最前線 査読有り

    湯川博、馬場嘉信

    ライフライン21 がんの先進医療   13 巻   頁: 31-34   2014年4月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 31-34

  431. Hepatic Monoacylglycerol O-acyltransferase 1 as a Promising Therapeutic Target for Steatosis, Obesity, and Type 2 Diabetes 査読有り

    Yasuhiro Hayashi, Erina Suemitsu, Kazuaki Kajimoto, Yusuke Sato, Afsana Akhter, Erina Suemitsu, Yu Sakurai, Hiroto Hatakeyama, Mamoru Hyodo, Noritada Kaji, Yoshinobu Baba, and Hideyoshi Harashima

    Mol. Ther., Nucleic Acids   3 巻   頁: e154   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/mtna.2014.4.

  432. Hepatic Monoacylglycerol O-acyltransferase 1 as a Promising Therapeutic Target for Steatosis, Obesity, and Type 2 Diabetes 査読有り

    Yasuhiro Hayashi, Erina Suemitsu, Kazuaki Kajimoto, Yusuke Sato, Afsana Akhter, Erina Suemitsu, Yu Sakurai, Hiroto Hatakeyama, Mamoru Hyodo, Noritada Kaji, Yoshinobu Baba, and Hideyoshi Harashima

      4 巻   頁: 5252   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/srep05252

  433. On-Chip Analysis of Intermittent Molecular Encounters in Nuclease Digestion of Specific DNA Sequence

    Onoshima Daisuke, Kaji Noritada, Tokeshi Manabu, Baba Yoshinobu

    BIOPHYSICAL JOURNAL   106 巻 ( 2 ) 頁: 699A-699A   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  434. 量子ドットイメージング 査読有り

    湯川博、馬場嘉信

    Organ Biology   21 巻   頁: 237-240   2014年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: n/a

  435. Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells 査読有り

    Hiroshi Yukawa a, Shingo Nakagawa b, Yasuma Yoshizumi b, Masaki Watanabe c, Hiroaki Saito d, Yoshitaka Miyamoto e, Hirofumi Noguchi f, Koichi Oishi g, Kenji Ono g, Makoto Sawada g, Ichiro Kato d, Daisuke Onoshima h, Momoko Obayashi a, Yumi Hayashi b, Noritada Kaji a, c, Tetsuya Ishikawa b, Shuji Hayashi e, Yoshinobu Baba a, b, i

    PLOS ONE     頁: DOI   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: DOI

  436. 肝がん細胞由来エクソソーム凍結保存の血管形成機能に及ぼす影響

    湯川 博, 馬場 嘉信

    Organ Biology   21 巻 ( 2 ) 頁: 237-240   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In this study, we herein examined the effects of the exosomes secreted from a stable hepatocellular carcinoma (HCC) cell line (HCC-exosomes) on the lumen formation of human umbilical vein endothelial cells(HUVECs). The uptake of HCC-exosomes by HUVECs and lumen formation by the HUVECs that included the exosomes were observed. The degree of lumen formation of HUVECs was dependent on the number of the HCC-exosomes. HCC-exosomes expressed a stress-induced heat shock protein associated with angiogenesis through the vascular endothelial growth factor(VEGF)receptor. In addition, the exosomes contained several microRNAs (miRNAs)reported to exist in the serum of HCC patients. The changes in the expression levels of miRNAs associated with angiogenesis were detected in the HUVECs treated with HCC-exosomes. Moreover, the abilities of the exosomes maintained after the treatment of cryopreservation. These results suggest that the HCC-exosomes play an important role in the angiogenesis. Further studies on the function of HCC-exosomes may provide a new target for HCC treatment.

    DOI: 10.11378/organbio.21.237

    CiNii Article

  437. Induced Pluripotent Stem Cell Labeling Using Quantum Dots.

    Yukawa H, Suzuki K, Kano Y, Yamada T, Kaji N, Ishikawa T, Baba Y

    Cell medicine   6 巻 ( 1-2 ) 頁: 83 - 90   2013年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3727/215517913X674270

    PubMed

  438. Adipose Tissue-Derived Stem Cell Imaging Using Cadmium-Free Quantum Dots.

    Miyazaki Y, Yukawa H, Nishi H, Okamoto Y, Kaji N, Torimoto T, Baba Y

    Cell medicine   6 巻 ( 1-2 ) 頁: 91 - 7   2013年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3727/215517913X674261

    PubMed

  439. Reduced Plasma Glucose by Asparagine Synthetase Knockdown in the Mouse Liver

    Iida Shinya, Kamiya Hiroyuki, Nakaya Akihiro, Hayashi Yasuhiro, Sawada Akihiro, Kaji Noritada, Baba Yoshinobu, Harashima Hideyoshi

    BIOLOGICAL & PHARMACEUTICAL BULLETIN   36 巻 ( 12 ) 頁: 2009-2011   2013年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  440. Observation of Positively Charged Magnetic Nanoparticles Inside HepG2 Spheroids Using Electron Microscopy.

    Miyamoto Y, Koshidaka Y, Noguchi H, Oishi K, Saito H, Yukawa H, Kaji N, Ikeya T, Suzuki S, Iwata H, Baba Y, Murase K, Hayashi S

    Cell medicine   5 巻 ( 2-3 ) 頁: 89 - 96   2013年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3727/215517913X666530

    PubMed

  441. Induced Pluripotent Stem Cell Labeling Using Quantum Dots 査読有り

    Hiroshi Yukawa, Kaoru Suzuki, Yuki Kano, Tatsuya Yamada, Noritada Kaji, Tetsuya Ishikawa, and Yoshinobu Baba

    Cell Medicine   6 巻   頁: 83-90   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  442. Adipose Tissue-Derived Stem Cell Imaging Using Cadmium-Free Quantum Dots 査読有り

    Yoshiyuki Miyazaki, Hiroshi Yukawa, Hiroyasu Nishi, Yukihiro Okamoto, Noritada Kaji, Tsukasa Torimoto, and Yoshinobu Baba

    Cell Medicine   6 巻   頁: 91-97   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  443. MICROFLUIDIC PARALLEL STRETCHING AND STAMPING OF SINGLE DNA MOLECULES FOR SUPER RESOLUTION MICROSCOPE IMAGING 査読有り

    Hirotoshi Yasaki, Daisuke Onoshima, Takao Yasui, Toyohiro Naito, Noritada Kaji, and Yoshinobu Baba

    Micro Total Analysis Systems 2013     頁: 88-90   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 88-90

  444. DEVELOPMENT OF 3RD GENERATION IMMUNO-PILLAR DEVICE FOR HIGH SENSITIVE DETECTION OF DISEASE MARKERS 査読有り

    N. Nishiwaki, T. Kasama, A. Ishida, H. Tani, Y. Baba, M. Tokeshi

    Micro Total Analysis Systems 2013     頁: 1164-1166   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 1164-1166

  445. CHRISTMAS-TREE NANOWIRE CHIPS FOR DNA SEPARATION 査読有り

    Sakon Rahong, Takao Yasui, Takeshi Yanagida, Masaki Kanai, Kazuki Nagashima, Annop Klamchuen, Meng Gang, He Yong, Fuwei Zhuge, Noritada Kaji, Yoshinobu Baba and Tomoji Kawai

    Micro Total Analysis Systems 2013     頁: 164-166   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  446. NANOWIRE DEVICES FOR EXOSOMAL MICRORNA EXTRACTION 査読有り

    Satoru Ito, Takao Yasui, He Yong, Takeshi Yanagida, Sakon Rahong, Masaki Kanai, Kazuki Nagashima, Hiroshi Yukawa, Noritada Kaji, Tomoji Kawai, Yoshinobu Baba

    Micro Total Analysis Systems 2013     頁: 1887-1889   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 1887-1889

  447. LABAL-FREE DETECTION AND QUANTIFICATION OF REAL-TIME DNA AMPLIFICATION USING ONE-DIMENSIONAL PHOTONIC CRYSTAL

    Takao Yasui, Kensuke Ogawa, Noritada Kaji, Mats Nilsson, Manabu Tokeshi, Yasuhiro Horiike, and Yoshinobu Baba

    Micro Total Analysis Systems 2013     頁: 1123-1125   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 1123-1125

  448. SINGLE DNA MOLECULE EXTRACTION FROM SINGLE BACTERIUM USING NANOWIRE STRUCTURES

    Kouhei Ootsuka, Takao Yasui, Noritada Kaji, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Kazuki Nagashima, Tomoji Kawai and Yoshinobu Baba

    Micro Total Analysis Systems 2013     頁: 479-481   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 479-481

  449. MICROFLUIDIC SINGLE-MOLECULE NUCLEASE DIGESTION REVEALS RATE-ENHANCING OFF-AND-ON MOLECULAR ENCOUNTERING FUNCTION FOR SITE-SPECIFIC DNA BREAK 査読有り

    Daisuke Onoshima, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

    Micro Total Analysis Systems 2013     頁: 820-822   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 820-822

  450. HIGH THROUGHPUT PURIFICATION DEVICES FOR IN VIVO APPLICATIONS OF GENEDELIVERY MULTIFUNCTIONAL EVELOPE-TYPE NANODEVICES 査読有り

    Noritada Kaji, Daisuke Shigenaka, Masami Ukawa, Manabu Tokeshi, Hidetaka Akita, Hideyoshi Harashima and Yoshinobu Baba

    Micro Total Analysis Systems 2013     頁: 1170-1172   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 1170-1172

  451. CONTROL OF DNA TRANSLOCATION VELOCITIES FOR NANOPORE-BASED DNA SEQUENCING 査読有り

    Xiaoyin Sun, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Noritada Kaji, Masaki Kanai, Kazuki Nagashima, Tomoji Kawai and Yoshinobu Baba

    Micro Total Analysis Systems 2013     頁: 1117-1119   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 1117-1119

  452. HIGH-SPEED MICRO-RNA ISOLATION FROM DNA FRAGMENTS BY NANOPILLER ARRAY CHIP 査読有り

    .Qiong Wu, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Noritada Kaji, Manabu Tokeshi, Kazuki Nagashima, Tomoji Kawai, and Yoshinobu Baba

    Micro Total Analysis Systems 2013     頁: 1206-1208   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 1206-1208

  453. DNA METHYLATION MAPPING IN NANOSLIT DEVICES AT A SINGLE MOLECULE LEVELMicro Total Analysis Systems 2013, 2013, 29-31. 査読有り

    Mayu Mizutani, Takao Yasui, Kasama Toshihiro, Nortada Kaji, Yoshinobu Baba

    Micro Total Analysis Systems 2013     頁: 29-31   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 29-31

  454. Visualization of Two-Dimensional Excitation Temperatures in CH4/N2/Ar Plasmas for Preparation of Carbonaceous Materials 査読有り

    Qingchun Shen, Yasushi Miyata, Shigeaki Morita, Yoshinobu Baba, Kuniyuki Kitagawa and Ashwani K. Gupta

    Journal of Energy Resources Technology   135 巻 ( 3 ) 頁: 034501-1,2, 3   2013年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1115/1.4023744

  455. Microfluidic biosensor for the detection of DNA by fluorescence enhancement and the following streptavidin detection by fluorescence quenching

    Jun Wang, Michihiko Aki, Daisuke Onoshima, Kenji Arinag Noritada Kaji Manabu Tokeshi,Shozo Fujita, Naoki Yokoyama, and Yoshinobu Baba

    Biosensors and Bioelectronics   ( 5 ) 頁: 280-285   2013年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  456. Plasmonic staining of DNA molecules with photo-induced Ag nanoparticles monitored using dark-field microscopy. 国際誌

    Yamamoto YS, Hirano K, Ishido T, Yasui T, Murase N, Baba Y, Itoh T

    Physical chemistry chemical physics : PCCP   15 巻 ( 25 ) 頁: 10316 - 20   2013年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Physical Chemistry Chemical Physics  

    DOI: 10.1039/c3cp51494c

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  457. High speed DNA denaturation using microheating devices 査読有り

    M. Furuhashi, Y. Okamoto, D. Onoshima, T. Ohshiro, S. Ryuzaki, K. Yokota, M. Tsutsui, M. Taniguchi, K. Nakatani, Y. Baba, T. Kawai

    Applied Physics Letters   103 巻 ( 2 ) 頁: 023112-1,2,3,4   2013年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  458. Nanopillar array chip integrated with on-line stacking for fast DNA separation with high sensitivity and high resolution

    Yasui T.

    Microfluidics and Nanofluidics   14 巻 ( 6 ) 頁: 961 - 967   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Microfluidics and Nanofluidics  

    DOI: 10.1007/s10404-012-1103-7

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  459. Nanobiodevices for Biomolecule Analysis and Imaging

    T. Yasui, N. Kaji, Y. Baba

    Annual Review of Analytical Chemistry   6 巻   頁: 83-96   2013年6月

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    記述言語:英語  

  460. Application of IgY to ELISA, LFDs, and immunopillar chips for detecting staphylococcal enterotoxins in milk and dairy products

    Jin W., Yamada K., Ikami M., Kaji N., Tokeshi M., Atsumi Y., Mizutani M., Murai A., Okamoto A., Namikawa T., Baba Y., Ohta M.

    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS   42 巻   頁: S46-S47   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  461. Plasmonic imaging of brownian motion of single DNA molecules spontaneously binding to Ag nanoparticles.

    Hirano K, Ishido T, Yamamoto YS, Murase N, Ichikawa M, Yoshikawa K, Baba Y, Itoh T

    Nano letters   13 巻 ( 5 ) 頁: 1877 - 82   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nano Letters  

    DOI: 10.1021/nl304247n

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  462. マウス尿中肺がんマーカー候補選定のための前処理法

    花井 陽介, 馬場 嘉信

    分析化学 = Japan analyst   62 巻 ( 5 ) 頁: 437 - 442   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:公益社団法人 日本分析化学会  

    Lung cancer is a leading cause of cancer-related deaths. Hence, developing early stage diagnostic tests that are highly sensitive and specific is crucial. In order to achieve a desirable preventive medicine in the future, a non-invasive diagnostic technique with a volatile biomarker is a key. In this work, we compared VOCs obtained from the urine of tumor-bearing mice and normal control mice, and found 21 VOCs to be candidate biomarkers for lung cancer. To more efficiently extract the candidate biomarkers, we tested five different pretreatment methods. Among these the most improved extraction efficiency was the addition of sodium chloride along with a heat treatment.

    DOI: 10.2116/bunsekikagaku.62.437

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  463. マウス尿中肺がんマーカー候補選定のための前処理法 査読有り

    分析化学   62 巻 ( 5 ) 頁: 437-442   2013年5月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  464. Plasmonic staining of DNA molecules with photo-induced Ag nanoparticles monitored using dark-field microscopy 査読有り

    Y.S. Yamamoto, K. Hirano, T. Ishido, T. Yasui, N. Murase, Y. Baba

    Physical Chemistry Chemical Physics   15 巻 ( 25 ) 頁: 10316-10320   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  465. Plasmonic Imaging of Brownian Motion of Single DNA Molecules Spontaneously Binding to Ag Nanoparticles 査読有り

    K. Hirano, T. Ishido, Y.S. Yamamoto, N. Murase, M. Ichikawa, K. Yoshikawa, Y. Baba

    Nano Lett.   13 巻 ( 5 ) 頁: 1877-1882   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  466. Nano Packagingが拓く次世代核酸医療 査読有り

    松尾保隆、加地範匡、畠山浩人、渡慶次学、小暮健太朗、馬場嘉信、原島秀吉

    表面   51 巻   頁: 227-240   2013年5月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 227-240

  467. Pretreatment Methods for Selections Lung Cancer Markers in Mouse Urine

    Hanai Yosuke, Baba Yoshinobu

    BUNSEKI KAGAKU   62 巻 ( 5 ) 頁: 437-442   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  468. DNA manipulation and separation in sublithographic-scale nanowire array.

    Yasui T, Rahong S, Motoyama K, Yanagida T, Wu Q, Kaji N, Kanai M, Doi K, Nagashima K, Tokeshi M, Taniguchi M, Kawano S, Kawai T, Baba Y

    ACS nano   7 巻 ( 4 ) 頁: 3029 - 35   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/nn4002424

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  469. Nanobiotechnology meets plant cell biology: Carbon nanotubes as organelle targeting nanocarriers

    Serag M.F.

    RSC Advances   3 巻 ( 15 ) 頁: 4856 - 4862   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:RSC Advances  

    DOI: 10.1039/c2ra22766e

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  470. SPME GC-TOF MSを用いた肺がん識別のための尿中揮発性有機化合物のスクリーニング法の検討

    花井 陽介, 馬場 嘉信

    Journal of the Mass Spectrometry Society of Japan   61 巻 ( 2 ) 頁: 13-21   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Of all types of cancers, lung cancer is the primary cause of mortality. To reduce the high mortality rate, development of sensitive and specific techniques for the early diagnosis of lung cancer would be highly desirable. For preventive medicine in the future, one of the key technologies is a non-invasive diagnostic technique for identifying volatile biomarkers. In this work, volatile organic compounds (VOCs) derived from urine were extracted by solid phase micro extraction (SPME) and analyzed by gas chromatography time-of-flight mass spectrometer (GC-TOF MS). We compared the VOCs obtained from the urine of the tumor-bearing mice with the corresponding compounds from normal control mice. In order to detect volatile biomarkers for lung cancer more efficiently, we examined five different pretreatment methods for the extraction of VOCs by SPME, and finally were able to identify some potential candidate biomarkers for lung cancer.

    DOI: 10.5702/massspec.12-49

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  471. DNA Manipulation and Separation in Sublithographic-Scale Nanowire Array 査読有り

    T. Yasui, S. Rahong, K. Motoyama, T. Yanagida, Q. Wu, N. Kaji, M. Kanai, K. Doi, K. Nagashima, M. Tokeshi, M. Taniguchi, S. Kawano, T. Kawai, Y. Baba

    ACS Nano   7 巻 ( 4 ) 頁: 3029-3035   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  472. SPME GC-TOF MSを用いた肺がん識別のため尿中揮発性有機化合物のスクリーニング法の検討 査読有り

    花井陽介, 馬場嘉信

    質量分析学会誌   61 巻 ( 2 ) 頁: 13-21   2013年4月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  473. Nanobiotechnology meets plant cell biology: carbon nanotubes as organelle targeting nanocarriers 査読有り

    M.F. Serag, N. Kaji, S. Habuchi, A. Bianco, Y. Baba

    RSC Advances   3 巻 ( 15 ) 頁: 4856-4862   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  474. Application of IgY to sandwich enzyme-linked immunosorbent assays, lateral flow devices, and immunopillar chips for detecting staphylococcal enterotoxins in milk and dairy products 査読有り

    W.C. Jin, K. Yamada, M. Ikami, N. Kaji, M. Tokeshi, Y. Atsumi, M. Mizutani, A. Murai, A. Okamoto, T. Namikawa, Y. Baba, M. Ohta

    Journal of Microbiological Methods   92 巻 ( 3 ) 頁: 323-331   2013年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  475. Parallel Real-Time PCR on a Chip for Genetic Tug-of-War (gTOW) Method 査読有り

    T. Naito, A. Yatsuhashi, N. Kaji, T. Ando, K. Sato, H. Moriya, H. Kitano, T. Yasui, M. Tokeshi, Y. Baba

    Analytical Sciences   29 巻 ( 3 ) 頁: 367-371   2013年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  476. Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules 査読有り

    T. Yasui, K. Motoyma, N. Kaji, M. Tokeshia, Y. Baba

    RSC Adv.   3 巻 ( 10 ) 頁: 3237–3240   2013年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  477. ナノバイオデバイスが拓く未来ヘルスケアとバイオセンサへの展開

    馬場嘉信

    月刊ディスプレイ   19 巻 ( 3 ) 頁: 1-8   2013年3月

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    記述言語:日本語  

  478. Application of IgY to sandwich enzyme-linked immunosorbent assays, lateral flow devices, and immunopillar chips for detecting staphylococcal enterotoxins in milk and dairy products. 国際誌

    Jin W, Yamada K, Ikami M, Kaji N, Tokeshi M, Atsumi Y, Mizutani M, Murai A, Okamoto A, Namikawa T, Baba Y, Ohta M

    Journal of microbiological methods   92 巻 ( 3 ) 頁: 323 - 31   2013年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.mimet.2013.01.001

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  479. Parallel Real-Time PCR on a Chip for Genetic Tug-of-War (gTOW) Method

    Naito Toyohiro, Yatsuhashi Ai, Kaji Noritada, Ando Taeko, Sato Kazuo, Moriya Hisao, Kitano Hiroaki, Yasui Takao, Tokeshi Manabu, Baba Yoshinobu

    ANALYTICAL SCIENCES   29 巻 ( 3 ) 頁: 367-371   2013年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  480. Temperature-driven self-actuated microchamber sealing system for highly integrated microfluidic devices.

    Naito T, Arayanarakool R, Le Gac S, Yasui T, Kaji N, Tokeshi M, van den Berg A, Baba Y

    Lab on a chip   13 巻 ( 3 ) 頁: 452 - 8   2013年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/c2lc41030c

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  481. Temperature-driven self-actuated microchamber sealing system for highly integrated microfluidic devices 査読有り

    T. Naito, R. Arayanarakool, S. Le Gac, T. Yasui, N. Kaji, M. Tokeshi, A. van den Berg, Y. Baba

    Lab on a Chip   13 巻 ( 3 ) 頁: 452-458   2013年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  482. カーボンナノチューブによる植物細胞への遺伝子導入

    馬場嘉信

    VBLニュース   17 巻 ( 2 ) 頁: 4   2013年2月

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    記述言語:日本語  

  483. 血中循環がん細胞(CTC)検出によるがん診断

    岡本行広, 馬場嘉信

    現代化学   504 巻   頁: 32-36   2013年2月

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    記述言語:日本語  

  484. 進展する早期がん診断の研究

    馬場嘉信

    現代化学   504 巻   頁: 21-23   2013年2月

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    記述言語:日本語  

  485. Simultaneous Immunoassay Analysis of Plasma IL-6 and TNF-alpha on a Microchip 査読有り

    K. Abe, Y. Hashimoto, S. Yatsushiro, S. Yamamura, M. Bando, Y. Hiroshima, J. Kido, M. Tanaka, Y. Shinohara, T. Ooie, Y. Baba, M. Kataoka

    PLoS One   8 巻 ( 1 ) 頁: 8   2013年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  486. A DNA microarray-based analysis of immune-stimulatory and transcriptional responses of dendritic cells to KALA-modified nanoparticles 査読有り

    H. Akita, S. Ishii, N. Miura, S.M. Shaheen, Y. Hayashi, T. Nakamura, N. Kaji, Y. Baba

    Biomaterials     頁: in press   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  487. On-demand Production of Highly Reproducible SERS Substrates Made of Silver Nanohexagonal Columns via Galvanic Displacement 査読有り

    Y.S. Yamamoto, K. Hasegawa, Y. Hasegawa, Y. Kitahama, S. Fukuoka, N. Murase, Y. Baba, Y. Ozaki, T. Itoh

    Physical Chemistry Chemical Physics     頁: in press   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  488. Reduced plasma glucose by asparagine synthetase knockdown in the mouse liver 査読有り

    Shinya Iida, Hiroyuki Kamiya, Akihiro Nakaya, Yasuhiro Hayashi, Akihiro Sawada, Noritada Kaji, Yoshinobu Baba and Hideyoshi Harashima

    Biol. Pharm. Bull., in press     頁: -   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: -

  489. Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

    Yasui Takao, Motoyama Koki, Kaji Noritada, Tokeshi Manabu, Baba Yoshinobu

    RSC ADVANCES   3 巻 ( 10 ) 頁: 3237 - 3240   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/c3ra22999h

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  490. ESTABLISHMENT OF PORTABLE IMMUNOASSAY SYSTEM FOR EARLY DIAGNOSIS

    Kasama Toshihiro, Hasegawa Yutaka, Matsumoto Hiroyuki, Kondo Haruyasu, Ozawa Tsutomu, Kaji Noritada, Tokeshi Manabu, Baba Yoshinobu

    2013 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)     頁: .   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Web of Science

  491. Nanobiodevices for biomolecule analysis and imaging. 国際誌

    Yasui T, Kaji N, Baba Y

    Annual review of analytical chemistry (Palo Alto, Calif.)   6 巻   頁: 83 - 96   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Annual Review of Analytical Chemistry  

    DOI: 10.1146/annurev-anchem-062012-092619

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  492. Parallel real-time PCR on a chip for genetic tug-of-war (gTOW) method.

    Naito T, Yatsuhashi A, Kaji N, Ando T, Sato K, Moriya H, Kitano H, Yasui T, Tokeshi M, Baba Y

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   29 巻 ( 3 ) 頁: 367 - 71   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:日本分析化学会  

    A microchip-based real-time polymerase chain reaction (PCR) device has been developed for the genetic tug-of-war (gTOW) method that provides quantitative data for research on biorobustness and systems biology. The device was constructed of a silicon glass chip, a temperature controlling Peltier element, and a microscope. A parallel real-time amplification process of target genes on the plasmids and the housekeeping genes in a model eukaryote <i>Saccharomyces cerevisiae</i> were detected simultaneously, and the copy number of the target genes were estimated. The device provides unique quantitative data that can be used to augment understanding of the system-level properties of living cells.

    DOI: 10.2116/analsci.29.367

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  493. Simultaneous immunoassay analysis of plasma IL-6 and TNF-α on a microchip.

    Abe K, Hashimoto Y, Yatsushiro S, Yamamura S, Bando M, Hiroshima Y, Kido J, Tanaka M, Shinohara Y, Ooie T, Baba Y, Kataoka M

    PloS one   8 巻 ( 1 ) 頁: e53620   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1371/journal.pone.0053620

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  494. 連載 超解像顕微鏡の世界:波長の壁を越えて見る(1)  超解像顕微鏡とは:Abbeの法則への挑戦

    加地範匡、渡慶次学、馬場嘉信

    現代化学   1 巻   頁: 50-54   2012年12月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    DOI: 50-54

  495. Spatiotemporal visualization of subcellular dynamics of carbon nanotubes.

    Serag MF, Braeckmans K, Habuchi S, Kaji N, Bianco A, Baba Y

    Nano letters   12 巻 ( 12 ) 頁: 6145 - 51   2012年12月

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    DOI: 10.1021/nl3029625

    PubMed

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    馬場嘉信

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    DOI: 10.1021/ac3020565

    Web of Science

    Scopus

    PubMed

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