研究者詳細 - 柴田　秀樹

論文 - 柴田　秀樹

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The calcium-binding protein ALG-2 regulates protein secretion and trafficking via interactions with MISSL and MAP1B proteins 査読有り国際誌 Open Access

Takahara, T; Inoue, K; Arai, Y; Kuwata, K; Shibata, H; Maki, M

JOURNAL OF BIOLOGICAL CHEMISTRY 292 巻 ( 41 ) 頁： 17057 - 17072 2017年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Biological Chemistry

Mobilization of intracellular calcium is essential for a wide range of cellular processes, including signal transduction, apo-ptosis, and vesicular trafficking. Several lines of evidence have suggested that apoptosis-linked gene 2 (ALG-2, also known as PDCD6), a calcium-binding protein, acts as a calcium sensor linking calcium levels with efficient vesicular trafficking, especially at the endoplasmic reticulum (ER)-to-Golgi transport step. However, how ALG-2 regulates these processes remains largely unclear. Here, we report that MAPK1-interacting and spindle-stabilizing (MISS)-like (MISSL), a previously uncharacterized protein, interacts with ALG-2 in a calcium-dependent manner. Live-cell imaging revealed that upon a rise in intracellular calcium levels, GFP-tagged MISSL (GFP-MISSL) dynamically relocalizes in a punctate pattern and colocalizes with ALG-2. MISSL knockdown caused disorganization of the components of the ER exit site, the ER-Golgi intermediate compartment, and Golgi. Importantly, knockdown of either MISSL or ALG-2 attenuated the secretion of secreted alkaline phospha-tase (SEAP), a model secreted cargo protein, with similar reductions in secretion by single- and double-protein knockdowns, suggesting that MISSL and ALG-2 act in the same pathway to regulate the secretion process. Furthermore, ALG-2 or MISSL knockdown delayed ER-to-Golgi transport of procollagen type I. We also found that ALG-2 and MISSL interact with microtubule-associated protein 1B (MAP1B) and that MAP1B knockdown reverts the reduced secretion of SEAP caused by MISSL or ALG-2 depletion. These results suggest that a change in the intracellular calcium level plays a role in regulation of the secretory pathway via interaction of ALG-2 with MISSL and MAP1B.

DOI： 10.1074/jbc.M117.800201

Web of Science

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PubMed

Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis 査読有り Open Access

Smith, BN; Topp, SD; Fallini, C; Shibata, H; Chen, HJ; Troakes, C; King, A; Ticozzi, N; Kenna, KP; Soragia-Gkazi, A; Miller, JW; Sato, A; Dias, DM; Jeon, M; Vance, C; Wong, CH; de Majo, M; Kattuah, W; Mitchell, JC; Scotter, EL; Parkin, NW; Sapp, PC; Nolan, M; Nestor, PJ; Simpson, M; Weale, M; Lek, M; Baas, F; de Jong, JMV; ten Asbroek, ALMA; Redondo, AG; Esteban-Pérez, J; Tiloca, C; Verde, F; Duga, S; Leigh, N; Pall, H; Morrison, KE; Al-Chalabi, A; Shaw, PJ; Kirby, J; Turner, MR; Talbot, K; Hardiman, O; Glass, JD; de Belleroche, J; Maki, M; Moss, SE; Miller, C; Gellera, C; Ratti, A; Al-Sarraj, S; Brown, RH; Silani, V; Landers, JE; Shaw, CE

SCIENCE TRANSLATIONAL MEDICINE 9 巻 ( 388 ) 頁： eaad9157 2017年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Science Translational Medicine

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder. We screened 751 familial ALS patient whole-exome sequences and identified six mutations including p.D40G in the ANXA11 gene in 13 individuals. The p.D40G mutation was absent from 70,000 control whole-exome sequences. This mutation segregated with disease in two kindreds and was present in another two unrelated cases (P = 0.0102), and all mutation carriers shared a common founder haplotype. Annexin A11-positive protein aggregates were abundant in spinal cord motor neurons and hippocampal neuronal axons in an ALS patient carrying the p.D40G mutation. Transfected human embryonic kidney cells expressing ANXA11 with the p.D40G mutation and other N-terminal mutations showed altered binding to calcyclin, and the p.R235Q mutant protein formed insoluble aggregates. We conclude that mutations in ANXA11 are associated with ALS and implicate defective intracellular protein trafficking in disease pathogenesis. 2017

DOI： 10.1126/scitranslmed.aad9157

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The calcium-binding protein ALG-2 promotes endoplasmic reticulum exit site localization and polymerization of Trk-fused gene (TFG) protein 査読有り国際誌 Open Access

Kanadome, T; Shibata, H; Kuwata, K; Takahara, T; Maki, M

FEBS JOURNAL 284 巻 ( 1 ) 頁： 56 - 76 2017年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：FEBS Journal

Apoptosis-linked gene 2 (ALG-2), which is a gene product of PDCD6, is a 22-kDa Ca2+-binding protein. Accumulating evidence points to a role for ALG-2 as a Ca2+-responsive adaptor protein. On binding to Ca2+, ALG-2 undergoes a conformational change that facilitates its interaction with various proteins. It also forms a homodimer and heterodimer with peflin, a paralog of ALG-2. However, the differences in cellular roles for the ALG-2 homodimer and ALG-2/peflin heterodimer are unclear. In the present study, we found that Trk-fused gene (TFG) protein interacted with the ALG-2 homodimer. Immunostaining analysis revealed that TFG and ALG-2 partially overlapped at endoplasmic reticulum exit sites (ERES), a platform for COPII-mediated protein transport from the endoplasmic reticulum. Time-lapse live-cell imaging demonstrated that both green fluorescent protein-fused TFG and mCherry-fused ALG-2 are recruited to ERES after thapsigargin treatment, which raises intracellular Ca2+ levels. Furthermore, overexpression of ALG-2 induced the accumulation of TFG at ERES. TFG has an ALG-2-binding motif and deletion of the motif decreased TFG binding to ALG-2 and shortened its half-life at ERES, suggesting a critical role for ALG-2 in retaining TFG at ERES. We also demonstrated, by in vitro cross-linking assays, that ALG-2 promoted the polymerization of TFG in a Ca2+-dependent manner. Collectively, the results suggest that ALG-2 acts as a Ca2+-sensitive adaptor to concentrate and polymerize TFG at ERES, supporting a potential role for ALG-2 in COPII-dependent trafficking from the endoplasmic reticulum.

DOI： 10.1111/febs.13949

Web of Science

Scopus

PubMed

Multifaceted Roles of ALG-2 in Ca²⁺-Regulated Membrane Trafficking. 査読有り

Masatoshi Maki, Terunao Takahara, Hideki Shibata

International Journal of Molecular Sciences 17 巻 ( 9 ) 頁： E1401 2016年8月

Tubby-like protein superfamily member PLSCR3 functions as a negative regulator of adipogenesis in mouse 3T3-L1 preadipocytes by suppressing induction of late differentiation stage transcription factors. 査読有り

Akira Inokawa, Tatsutoshi Inuzuka, Terunao Takahara, Hideki Shibata, Masatoshi Maki

Bioscience Reports 36 巻 ( 1 ) 頁： e00287 2016年1月

A New Role for Annexin A11 in the Early Secretory Pathway via Stabilizing Sec31A Protein at the Endoplasmic Reticulum Exit Sites (ERES). 査読有り

Hideki Shibata, Takashi Kanadome, Hirofumi Sugiura, Takeru Yokoyama, Minami Yamamuro, Stephen E. Moss, Masatoshi Maki

The Journal of Biological Chemistry 290 巻 ( 8 ) 頁： 4981-4993 2015年2月

Structural Analysis of the Complex between Penta-EF-Hand ALG-2 Protein and Sec31A Peptide Reveals a Novel Target Recognition Mechanism of ALG-2 査読有り

Takeshi Takahashi, Kyosuke Kojima, Wei Zhang, Kanae Sasaki, Masaru Ito, Hironori Suzuki, Masato Kawasaki, Soichi Wakatsuki, Terunao Takahara, Hideki Shibata, Masatoshi Maki

International Journal of Molecular Sciences 16 巻 ( 2 ) 頁： 3677-3699 2015年2月

Involvement of calpain-7 in epidermal growth factor receptor degradation via the endosomal sorting pathway. 査読有り

Yuki Maemoto, Yasuko Ono, Satomi Kiso, Hideki Shibata, Terunao Takahara, Hiroyuki Sorimachi, Masatoshi Maki

FEBS J. 281 巻 ( 16 ) 頁： 3642-3655 2014年7月

Nuclear ALG-2 protein interacts with Ca2+ homeostasis endoplasmic reticulum protein (CHERP) Ca2+-dependently and participates in regulation of alternative splicing of inositol trisphosphate receptor type 1 (IP3R1) pre-mRNA. 査読有り

Kanae Sasaki-Osugi, Chiaki Imoto, Terunao Takahara, Hideki Shibata, Masatoshi Maki

J. Biol. Chem. 288 巻 ( 46 ) 頁： 33361-33375 2013年9月

VPS37 isoforms differentially modulate the ternary complex formation of ALIX, ALG-2, and ESCRT-I. 査読有り

Mayumi Okumura, Angela M. Katsuyama, Hideki Shibata, Masatoshi Maki

Biosci. Biotechnol. Biochem. 77 巻 ( 8 ) 頁： 1715-1721 2013年8月

Identification of phosphorylation sites in the C-terminal region of charged multivesicular body protein 1A (CHMP1A). 査読有り

Yuki Maemoto, Hideki Shibata, Masatoshi Maki

Biosci. Biotechnol. Biochem. 77 巻 ( 6 ) 頁： 1317-1319 2013年6月

Mammalian ESCRT-III-related protein IST1 has a distinctive Met-Pro repeat sequence that is essential for interaction with ALG-2 in the presence of Ca2+. 査読有り

Mayumi Okumura, Takeshi Takahashi, Hideki Shibata, Masatoshi Maki

Biosci. Biotechnol. Biochem. 77 巻 ( 5 ) 頁： 1049-1054 2013年5月

Analysis of limited proteolytic activity of calpain-7 using non-physiological substrates in mammalian cells. 査読有り

Yuki Maemoto, Satomi Kiso, Hideki Shibata, Masatoshi Maki

FEBS J. 280 巻 ( 11 ) 頁： 2594-2607 2013年4月

ALG-2-interacting Tubby-like protein superfamily member PLSCR3 is secreted by an exosomal pathway and taken up by recipient cultured cells. 査読有り

Tatsutoshi Inuzuka, Akira Inokawa, Cen Chen, Kumiko Kizu, Hiroshi Narita, Hideki Shibata, Masatoshi Maki

Biosci. Rep. 33 巻 ( 2 ) 頁： e00026 2013年3月

Biochemical and immunological detection of physical interactions between penta-EF-hand protein ALG-2 and its binding partners. 査読有り

Kanae Osugi, Hideki Shibata, Masatoshi Maki

Methods Mol Biol. 963 巻頁： 187-200 2012年11月

Prediction of a New Ligand-Binding Site for Type 2 Motif based on the Crystal Structure of ALG-2 by Dry and Wet Approaches. 査読有り

Takeshi Takahashi, Hironori Suzuki, Tatsutoshi Inuzuka, Hideki Shibata, Masatoshi Maki

Int J Mol Sci. 13 巻 ( 6 ) 頁： 7535-7549 2012年6月

Identification of the P-body component PATL1 as a novel ALG-2-interacting protein by in silico and far-Western screening of proline-rich proteins. 査読有り

Kanae Osugi, Hironori Suzuki, Tomomi Nomura, Yasuo Ariumi, Hideki Shibata, Masatoshi Maki

J Biochem. 151 巻 ( 6 ) 頁： 657-666 2012年6月

Evolutionary and physical linkage between calpains and penta-EF-hand Ca2+-binding proteins. 査読有り

Masatoshi Maki, Yuki Maemoto, Yohei Osako, Hideki Shibata

FEBS J. 279 巻 ( 8 ) 頁： 1414-1421 2012年4月

Calpain-7 binds to CHMP1B at its second α-helical region and forms a ternary complex with IST1. 査読有り

Yuki Maemoto, Yohei Osako, Emi Goto, Eri Nozawa, Hideki Shibata, Masatoshi Maki

J. Biochem. 150 巻 ( 4 ) 頁： 411-421 2011年10月

Structure and function of ALG-2, a penta-EF-hand calcium-dependent adaptor protein. 査読有り

Masatoshi Maki, Hironori Suzuki, Hideki Shibata

Sci China Life Sci. 54 巻 ( 8 ) 頁： 770-779 2011年8月

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