Updated on 2024/03/22

写真a

 
GOSHIMA, Gohta
 
Organization
Graduate School of Science Professor
Graduate School
Graduate School of Science
Undergraduate School
School of Science
Title
Professor

Degree 1

  1. 博士(理学) ( 2002.3 ) 

Research Interests 3

  1. スピンドル

  2. 微小管

  3. 細胞分裂

Research Areas 1

  1. Others / Others  / Cell Biology

Education 1

  1. Kyoto University   Graduate School, Division of Natural Science

    1999.4 - 2002.3

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    Country: Japan

Professional Memberships 2

  1. 日本細胞生物学会

  2. 日本分子生物学会

Awards 3

  1. 第15回(平成30年度)日本学術振興会賞

    2019.2   独立行政法人 日本学術振興会  

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    Country:Japan

  2. 井上リサーチアウォード

    2010.2   井上科学振興財団  

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    Country:Japan

  3. HFSP Career Development Award

    2008   HFSPO  

 

Papers 68

  1. Live-cell imaging under centrifugation characterized the cellular force for nuclear centration in the Caenorhabditis elegans embryo

    Goda M, Shribak M, Ikeda Z, Okada N, Tani T, Goshima G, Oldenbourg R, Kimura A.

    bioRxiv     2024.1

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    Language:English  

    DOI: 10.1101/2024.01.03.574024

  2. Genome sequence and cell biological toolbox of the highly regenerative, coenocytic green feather alga Bryopsis

    Ochiai KK, Hanawa D, Ogawa HA, Tanaka H, Uesaka K, Edzuka T, Shirae-Kurabayashi M, Toyoda A, Itoh T, Goshima G.

    bioRxiv     2023.11

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    Authorship:Last author, Corresponding author   Language:English  

    DOI: 10.1101/2023.11.22.568388

  3. <i>Physcomitrium patens</i> SUN2 Mediates MTOC Association with the Nuclear Envelope and Facilitates Chromosome Alignment during Spindle Assembly Reviewed

    Yoshida, MW; Oguri, N; Goshima, G

    PLANT AND CELL PHYSIOLOGY   Vol. 64 ( 9 ) page: 1106 - 1117   2023.9

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    Authorship:Last author, Corresponding author   Language:English  

    DOI: 10.1093/pcp/pcad074

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  4. Draft Genome Sequences of Two Dothideomycetes Strains, NU30 and NU200, Derived from the Marine Environment around Sugashima, Japan Reviewed

    Kurita, G; Goshima, G; Uesaka, K

    MICROBIOLOGY RESOURCE ANNOUNCEMENTS   Vol. 12 ( 5 )   2023.5

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  5. Armadillo repeat-containing kinesin represents the versatile plus-end-directed transporter in <i>Physcomitrella</i> Reviewed

    Yoshida, MW; Hakozaki, M; Goshima, G

    NATURE PLANTS   Vol. 9 ( 5 ) page: 733 - +   2023.5

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    Authorship:Last author, Corresponding author   Language:English  

    DOI: 10.1038/s41477-023-01397-x

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  6. Control of Plant Cell Growth and Proliferation by MO25A, a Conserved Major Component of the Mammalian Sterile 20–Like Kinase Pathway Reviewed International coauthorship

    Plant and Cell Physiology   Vol. 64 ( 3 ) page: 336 - 351   2023.3

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcad005

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  7. Armadillo repeat-containing kinesin ARK represents the versatile plus-end-directed transporter in plants

    Yoshida, M; Hakozaki, M; Goshima, G

    MOLECULAR BIOLOGY OF THE CELL   Vol. 34 ( 2 ) page: 497 - 498   2023.2

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  8. Spindle motility skews division site determination during asymmetric cell division in Physcomitrella

    Kozgunova, E; Yoshida, MW; Reski, R; Goshima, G

    NATURE COMMUNICATIONS   Vol. 13 ( 1 )   2022.5

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  9. Mitotic spindle formation in the absence of Polo kinase Invited Reviewed

    Kim J, Goshima G.

    Proc Natl Acad Sci USA   Vol. 119 ( 12 ) page: e2114429119   2022.3

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  10. Division site determination during asymmetric cell division in plants Reviewed

    Yi, PS; Goshima, G

    PLANT CELL   Vol. 34 ( 6 ) page: 2120 - 2139   2022.3

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  11. Physical properties of the cytoplasm modulate the rates of microtubule polymerization and depolymerization Reviewed

    Molines, AT; Lemière, J; Gazzola, M; Steinmark, IE; Edrington, CH; Hsu, CT; Real-Calderon, P; Suhling, K; Goshima, G; Holt, LJ; Thery, M; Brouhard, GJ; Chang, F

    DEVELOPMENTAL CELL   Vol. 57 ( 4 ) page: 466 - +   2022.2

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  12. Growth and division mode plasticity is dependent on cell density in marine-derived black yeasts Reviewed

    GENES TO CELLS   Vol. 27 ( 2 ) page: 124 - 137   2022.2

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  13. Fifteen compelling open questions in plant cell biology Reviewed

    Roeder Adrienne H. K., Otegui Marisa S., Dixit Ram, Anderson Charles T., Faulkner Christine, Zhang Yan, Harrison Maria J., Kirchhelle Charlotte, Goshima Gohta, Coate Jeremy E., Doyle Jeff J., Hamant Olivier, Sugimoto Keiko, Dolan Liam, Meyer Heather, Ehrhardt David W., Boudaoud Arezki, Messina Carlos

    PLANT CELL   Vol. 34 ( 1 ) page: 72 - 102   2022.1

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  14. Cell tip growth underlies injury response of marine macroalgae Invited Reviewed

    Shirae-Kurabayashi M, Edzuka T, Suzuki M, Goshima G.

    PLoS ONE   Vol. 17 ( 3 ) page: e0264827   2022

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  15. SS Microtubule-associated proteins promote microtubule generation in the absence of gamma-tubulin in human colon cancer cells Reviewed

    Tsuchiya Kenta, Goshima Gohta

    JOURNAL OF CELL BIOLOGY   Vol. 220 ( 12 )   2021.12

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  16. The 3D architecture and molecular foundations of de novo centriole assembly via bicentrioles Reviewed

    Pereira Sonia Gomes, Sousa Ana Laura, Nabais Catarina, Paixao Tiago, Holmes Alexander J., Schorb Martin, Goshima Gohta, Tranfield Erin M., Becker Jorg D., Bettencourt-Dias Monica

    CURRENT BIOLOGY   Vol. 31 ( 19 ) page: 4340 - +   2021.10

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  17. Plant stem cell research is uncovering the secrets of longevity and persistent growth Reviewed

    Umeda Masaaki, Ikeuchi Momoko, Ishikawa Masaki, Ito Toshiro, Nishihama Ryuichi, Kyozuka Junko, Torii Keiko U., Satake Akiko, Goshima Gohta, Sakakibara Hitoshi

    PLANT JOURNAL   Vol. 106 ( 2 ) page: 326 - 335   2021.4

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  18. Ran-GTP Is Non-essential to Activate NuMA for Mitotic Spindle-Pole Focusing but Dynamically Polarizes HURP Near Chromosomes Reviewed International journal

    Tsuchiya, K; Hayashi, H; Nishina, M; Okumura, M; Sato, Y; Kanemaki, MT; Goshima, G; Kiyomitsu, T

    CURRENT BIOLOGY   Vol. 31 ( 1 ) page: 115 - +   2021.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.cub.2020.09.091

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  19. Rho of Plants GTPases and Cytoskeletal Elements Control Nuclear Positioning and Asymmetric Cell Division during <i>Physcomitrella</i> <i>patens</i> Branching Reviewed International journal

    Yi, PS; Goshima, G

    CURRENT BIOLOGY   Vol. 30 ( 14 ) page: 2860 - +   2020.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.cub.2020.05.022

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  20. Transient cotransformation of CRISPR/Cas9 and oligonucleotide templates enables efficient editing of target loci in <i>Physcomitrella patens</i> Reviewed International journal

    Yi, PS; Goshima, G

    PLANT BIOTECHNOLOGY JOURNAL   Vol. 18 ( 3 ) page: 599 - 601   2020.3

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/pbi.13238

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  21. Kinesin-13 and Kinesin-8 Function during Cell Growth and Division in the Moss <i>Physcomitrella patens</i><SUP>[OPEN]</SUP> Reviewed International journal

    Leong, SY; Edzuka, T; Goshima, G; Yamada, M

    PLANT CELL   Vol. 32 ( 3 ) page: 683 - 702   2020.3

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1105/tpc.19.00521

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  22. A versatile microfluidic device for highly inclined thin illumination microscopy in the moss <i>Physcomitrella patens</i> Reviewed International journal

    Kozgunova, E; Goshima, G

    SCIENTIFIC REPORTS   Vol. 9   2019.10

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-019-51624-9

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  23. Editorial overview: Cell division - from molecules to tissues Invited International coauthorship International journal

    Goshima, G; Bellaïche, Y

    CURRENT OPINION IN CELL BIOLOGY   Vol. 60   page: III - V   2019.10

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ceb.2019.06.006

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  24. Kinetochore protein depletion underlies cytokinesis failure and somatic polyploidization in the moss <i>Physcomitrella patens</i> Reviewed International journal

    Kozgunova, E; Nishina, M; Goshima, G

    ELIFE   Vol. 8   2019.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.7554/eLife.43652

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  25. <i>Drosophila</i> kinesin-8 stabilizes the kinetochore-microtubule interaction Reviewed International journal

    Edzuka, T; Goshima, G

    JOURNAL OF CELL BIOLOGY   Vol. 218 ( 2 ) page: 474 - 488   2019.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1083/jcb.201807077

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  26. Moss Kinesin-14 KCBP Accelerates Chromatid Motility in Anaphase Reviewed International journal

    Yoshida, MW; Yamada, M; Goshima, G

    CELL STRUCTURE AND FUNCTION   Vol. 44 ( 2 ) page: 95 - 104   2019

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1247/csf.19015

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  27. Identification of 15 New Bypassable Essential Genes of Fission Yeast Reviewed International coauthorship International journal

    Takeda, A; Saitoh, S; Ohkura, H; Sawin, KE; Goshima, G

    CELL STRUCTURE AND FUNCTION   Vol. 44 ( 2 ) page: 113 - 119   2019

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1247/csf.19025

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  28. Microtubule nucleation and organization without centrosomes Invited Reviewed International journal

    Yi, PS; Goshima, G

    CURRENT OPINION IN PLANT BIOLOGY   Vol. 46   page: 1 - 7   2018.12

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    DOI: 10.1016/j.pbi.2018.06.004

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  29. The KCH Kinesin Drives Nuclear Transport and Cytoskeletal Coalescence to Promote Tip Cell Growth in Physcomitrella patens

    Yamada Moe, Goshima Gohta

    PLANT CELL   Vol. 30 ( 7 ) page: 1496 - 1510   2018.7

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    DOI: 10.1105/tpc.18.00038

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  30. SPIRAL2 Stabilises Endoplasmic Microtubule Minus Ends in the Moss <i>Physcomitrella patens</i> Reviewed International journal

    Leong, SY; Yamada, M; Yanagisawa, N; Goshima, G

    CELL STRUCTURE AND FUNCTION   Vol. 43 ( 1 ) page: 53 - 60   2018

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    DOI: 10.1247/csf.18001

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  31. Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2

    Tungadi Elsa A., Ito Ami, Kiyomitsu Tomomi, Goshima Gohta

    JOURNAL OF CELL SCIENCE   Vol. 130 ( 21 ) page: 3676 - 3684   2017.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/jcs.203703

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  32. Cytoplasmic MTOCs control spindle orientation for asymmetric cell division in plants

    Kosetsu Ken, Murata Takashi, Yamadaa Moe, Nishina Momoko, Boruc Joanna, Hasebe Mitsuyasu, Van Damme Daniel, Goshima Gohta

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 114 ( 42 ) page: E8847 - E8854   2017.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.1713925114

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  33. 14-3-3 regulation of Ncd reveals a new mechanism for targeting proteins to the spindle in oocytes

    Beaven Robin, Bastos Ricardo Nunes, Spanos Christos, Rome Pierre, Cullen C. Fiona, Rappsilber Juri, Giet Regis, Goshima Gohta, Ohkura Hiroyuki

    JOURNAL OF CELL BIOLOGY   Vol. 216 ( 10 ) page: 3029 - 3039   2017.10

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    DOI: 10.1083/jcb.201704120

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  34. Multiple kinesin-14 family members drive microtubule minus end-directed transport in plant cells

    Yamada Moe, Tanaka-Takiguchi Yohko, Hayashi Masahito, Nishina Momoko, Goshima Gohta

    JOURNAL OF CELL BIOLOGY   Vol. 216 ( 6 ) page: 1705 - 1714   2017.6

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    DOI: 10.1083/jcb.201610065

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  35. Mitotic Spindle Assembly in Land Plants: Molecules and Mechanisms Invited Reviewed International journal

    Yamada, M; Goshima, G

    BIOLOGY-BASEL   Vol. 6 ( 1 )   2017.3

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    DOI: 10.3390/biology6010006

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  36. Shortening of Microtubule Overlap Regions Defines Membrane Delivery Sites during Plant Cytokinesis

    de Keijzer Jeroen, Kieft Henk, Ketelaar Tijs, Goshima Gohta, Janson Marcel E.

    CURRENT BIOLOGY   Vol. 27 ( 4 ) page: 514 - 520   2017.2

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    DOI: 10.1016/j.cub.2016.12.043

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  37. Intra-spindle Microtubule Assembly Regulates Clustering of Microtubule-Organizing Centers during Early Mouse Development Reviewed

    Watanabe S, Shioi G, Furuta Y, Goshima G.

    Cell Rep   Vol. 15   page: 54-60   2016.4

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  38. Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission Reviewed

    Uehara R, Kamasaki T, Hiruma S, Poser I, Yoda K, Yajima J, Gerlich DW, Goshima G.

    Mol Biol Cell   Vol. 27   page: 812-27   2016.3

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  39. Live Cell Microscopy-Based RNAi Screening in the Moss Physcomitrella patens Invited

    Miki T, Nakaoka Y, Goshima G.

    Methods Mol Biol   Vol. 1470   page: 225-46   2016

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  40. Imaging Mitosis in the Moss Physcomitrella patens Invited

    Yamada M, Miki T, Goshima G.

    Methods Mol Biol   Vol. 1413   page: 263-82   2016

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  41. Five factors can reconstitute all three phases of microtubule polymerization dynamics Reviewed

    Moriwaki T, Goshima G

    J Cell Biol   Vol. 215 ( 3 ) page: 357   2016

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  42. The microtubule catastrophe promoter Sentin delays stable kinetochore-microtubule attachment in oocytes. Reviewed

    Głuszek AA, Cullen CF, Li W, Battaglia RA, Radford SJ, Costa MF, McKim KS, Goshima G, Ohkura H.

    J Cell Biol   Vol. 211   page: 1113-20   2015.12

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  43. Microcephaly protein Asp focuses the minus ends of spindle microtubules at the pole and within the spindle. Reviewed

    Ito A, Goshima G.

    J. Cell Biol.   Vol. 211   page: 999-1009   2015.12

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  44. Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants

    Jonsson E, Yamada M, Vale RD, Goshima G.

    Nature Plants   Vol. 1   page: 15087   2015.6

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  45. RNAi screening identifies the armadillo repeat-containing kinesins responsible for microtubule-dependent nuclear positioning in Physcomitrella patens.

    Miki T, Nishina M, Goshima G.

    Plant Cell Physiol.   Vol. 56 ( 4 ) page: 737-749   2015.1

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  46. Cytoplasmic nucleation and atypical branching nucleation generate endoplasmic microtubules in Physcomitrella patens Reviewed

    Nakaoka Y, Kimura A, Tani T, Goshima G.

    Plant Cell   Vol. 27 ( 1 ) page: 228-242   2015.1

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  47. NACK kinesin is required for metaphase chromosome alignment and cytokinesis in the moss Physcomitrella patens.

    Naito H, Goshima G.

    Cell Structure and Function.   Vol. 40 ( 1 ) page: 31-41   2015

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  48. Gohta Goshima: questing for answers on the mitotic spindle

    Goshima G, Sedwick C.

    J Cell Biol     2014.7

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    DOI: 10.1083/jcb.2062pi

  49. Identification of the augmin complex in the filamentous fungus Aspergillus nidulans.

    Edzuka T, Yamada L, Kanamaru K, Sawada H, Goshima G.

    PLoS One     2014.7

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    DOI: 10.1371/journal.pone.0101471

  50. Endogenous localizome identifies 43 mitotic kinesins in a plant cell

    Miki T, Naito H, Nishina M, Goshima G.

    Proc Natl Acad Sci U S A     2014.5

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    DOI: 10.1073/pnas.1311243111

  51. Friction on MAP determines its traveling direction on microtubules.

        2014.4

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    DOI: 10.1016/j.devcel.2014.03.022

  52. Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells

    Proc Natl Acad Sci U S A   Vol. 110 ( 49 ) page: 19808-13   2013.12

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  53. Loss of a Rho-Regulated Actin Nucleator, mDia2, Impairs cytokinesis during mouse fetal erythropoiesis.

    Watanabe S, De Zan T, Ishizaki T, Yasuda S, Kamijo H, Yamada D, Aoki T, Kiyonari H, Kaneko H, Shimizu R, Yamamoto M, Goshima G, Narumiya S.

      Vol. 5 ( 4 ) page: 926-32   2013.11

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  54. MICROTUBULE-ASSOCIATED PROTEIN65 is essential for maintenance of phragmoplast bipolarity and formation of the cell plate in Physcomitrella patens.

    Kosetsu K, de Keijzer J, Janson ME, Goshima G.

    Plant Cell     2013.11

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    DOI: 10.1105/tpc.113.117432

  55. Augmin-dependent microtubule nucleation at microtubule walls in the spindle. Reviewed

    Kamasaki T, O'Toole E, Kita S, Osumi M, Usukura J, McIntosh JR, Goshima G.

    J. Cell Biol.   Vol. 202 ( 1 ) page: 25-33   2013

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  56. Aurora B and Kif2A control microtubule length for assembly of a functional central spindle during anaphase. Reviewed

    Uehara R, Tsukada Y, Kamasaki T, Poser I, Yoda K, Gerlich DW, Goshima G.

      Vol. 202 ( 4 ) page: 623-36   2013

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  57. An inducible RNA interference system in Physcomitrella patens reveals a dominant role of augmin in phragmoplast microtubule generation Reviewed

    Nakaoka Y, Miki T, Fujioka R, Uehara R, Tomioka A, Obuse C, Kubo M, Hiwatashi Y, Goshima G.

    Plant Cell   Vol. 24 ( 4 ) page: 1478-93   2012.4

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  58. Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin Reviewed

    Li W, Moriwaki T, Tani T, Watanabe T, Kaibuchi K, Goshima G.

    J. Cell Biol.   Vol. 199 ( 5 ) page: 849-62   2012

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  59. Identification of a TPX2-like microtubule-associated protein in Drosophila. Reviewed

    Goshima G.

    PLoS One   Vol. 6   page: e28120   2011.11

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  60. EB1 promotes microtubule dynamics by recruiting Sentin in Drosophila cells. Reviewed

    Li W, Miki T, Watanabe T, Kakeno M, Sugiyama I, Kaibuchi K, Goshima G.

    J Cell Biol.   Vol. 193 ( 6 ) page: 973-983   2011.6

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  61. Control of mitotic spindle length. Invited Reviewed

    Goshima G, Scholey JM.

    Annu Rev Cell Dev Biol   Vol. 26   page: 21-57   2010.11

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  62. Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase. Reviewed

    Uehara R, Goshima G.

    Journal of Cell Biology   Vol. 191   page: 259-267   2010.10

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  63. Determinants of myosin II cortical localization during cytokinesis. Reviewed

    Uehara R, Goshima G, Mabuchi I, Vale RD, Spudich JA, Griffis ER.

    Current Biology   Vol. 22   page: 1080-1085   2010.6

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  64. *New look inside the spindle: microtubule-dependent microtuble generation within the spindle. Invited Reviewed

    Goshima G, Kimura A

    Current Opinion in Cell Biology   Vol. Epub   2010.2

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  65. *The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells. Reviewed

    Uehara R, Nozawa RS, Tomioka A, Petry S, Vale RD, Obuse C, Goshima G.

    Proc Natl Acad Sci U S A.   Vol. 106   page: 6998-7003   2009.4

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  66. *Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle Reviewed

    Gohta Goshima, Mirjam Mayer, Nan Zhang, Nico Stuurman, Ronald D. Vale

    Journal of Cell Biology   Vol. 181   page: 421-429   2008.5

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    Since the discovery of gamma-tubulin, attention has focused on its involvement as a microtubule nucleator at the centrosome. However, mislocalization of gamma-tubulin away from the centrosome does not inhibit mitotic spindle formation in Drosophila melanogaster, suggesting that a critical function for gamma-tubulin might reside elsewhere. A previous RNA interference (RNAi) screen identified five genes (Dgt2-6) required for localizing gamma-tubulin to spindle microtubules. We show that the Dgt proteins interact, forming a stable complex. We find that spindle microtubule generation is substantially reduced after knockdown of each Dgt protein by RNAi. Thus, the Dgt complex that we name "augmin" functions to increase microtubule number. Reduced spindle microtubule generation after augmin RNAi, particularly in the absence of functional centrosomes, has dramatic consequences on mitotic spindle formation and function, leading to reduced kinetochore fiber formation, chromosome misalignment, and spindle bipolarity defects. We also identify a functional human homologue of Dgt6. Our results suggest that an important mitotic function for gamma-tubulin may lie within the spindle, where augmin and gamma-tubulin function cooperatively to amplify the number of microtubules.

  67. Functional genomic screen reveals genes involved in lipid-droplet formation and utilization. Reviewed

    Guo Y, Walther TC, Rao M, Stuurman N, Goshima G, Terayama K, Wong JS, Vale RD, Walter P, Farese RV.

    Nature   Vol. 453 ( 7195 ) page: 657-661   2008.5

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    Eukaryotic cells store neutral lipids in cytoplasmic lipid droplets enclosed in a monolayer of phospholipids and associated proteins. These dynamic organelles serve as the principal reservoirs for storing cellular energy and for the building blocks for membrane lipids. Excessive lipid accumulation in cells is a central feature of obesity, diabetes and atherosclerosis, yet remarkably little is known about lipid-droplet cell biology. Here we show, by means of a genome-wide RNA interference (RNAi) screen in Drosophila S2 cells that about 1.5% of all genes function in lipid-droplet formation and regulation. The phenotypes of the gene knockdowns sorted into five distinct phenotypic classes. Genes encoding enzymes of phospholipid biosynthesis proved to be determinants of lipid-droplet size and number, suggesting that the phospholipid composition of the monolayer profoundly affects droplet morphology and lipid utilization. A subset of the Arf1-COPI vesicular transport proteins also regulated droplet morphology and lipid utilization, thereby identifying a previously unrecognized function for this machinery. These phenotypes are conserved in mammalian cells, suggesting that insights from these studies are likely to be central to our understanding of human diseases involving excessive lipid storage.

  68. *Genes required for mitotic spindle assembly in Drosophila S2 cells Reviewed

    Gohta Goshima, Roy Wollman, Sarah S. Goodwin, Nan Zhang, Jonathan M. Scholey, Ronald D. Vale and Nico Stuurman.

    Science   Vol. 316 ( 5823 ) page: 417-421   2007.7

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.

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Books 3

  1. 酵母の分裂様式の多様性・可塑性について

    栗田岳歩、五島剛太( Role: Joint author)

    2023.10 

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    Language:Japanese

    DOI: 10.34565/seibutsukogaku.101.10_528

  2. Assessment of mitotic spindle phenotypes in Drosophila S2 cells.

    Gohta Goshima( Role: Sole author)

    Methods Cell Biol.  2010 

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    Language:English

  3. RNAi in Drosophila S2 cells as a tool for studying cell cycle progression

    Bettencourt-Dias M, Goshima G.( Role: Joint author)

    Methods Mol Biol.  2009 

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    Language:English

Presentations 12

  1. Asymmetric cell division in the absence of centrosomes in plants Invited International conference

    Gohta Goshima

    EMBO Workshop "Centrosomes in development, disease and evolution"  2023.9.27 

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    Event date: 2023.9

    Language:English   Presentation type:Oral presentation (invited, special)  

  2. Mitotic cell division in Physcomitrella Invited International conference

    Gohta Goshima

    SEB Centenary Conference 2023  2023.7.5 

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    Event date: 2023.7

    Language:English   Presentation type:Oral presentation (invited, special)  

  3. Microtubule and motors in plants Invited International conference

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    Event date: 2023.2

    Language:English   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Country:France  

  4. Evolutionary replacement of genes required for cell division and intracellular transport. Invited International conference

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    Event date: 2023.2

    Language:English   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Country:Switzerland  

  5. Convention and novelty - studying typical cellular processes in atypical cell models Invited International conference

    2022.11.30 

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    Event date: 2022.11 - 2022.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  6. 細胞分裂の宝探し Invited

    五島剛太

    細胞分裂研究会  2022.7.28 

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    Event date: 2022.7

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  7. Growth and division mode plasticity is dependent on in marine-derived black yeasts

    2021.12.2 

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    Event date: 2021.12

    Presentation type:Oral presentation (general)  

    Country:Japan  

  8. Microtubule generation within the spindle International conference

    FASEB meeting “Mitotic spindle assembly and function" 

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    Event date: 2009.9

    Language:English   Presentation type:Oral presentation (general)  

  9. Genome-wide RNAi Screen Identifies Genes Required for Mitotic Spindle Formation in Animal Cells International conference

    9th EMBL-NIBB joint symposium on Functional Imaging 

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    Event date: 2009.4

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  10. Microtubule generation within the mitotic spindle

    2nd International Symposium on Bio-nanosystems 

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    Event date: 2008.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  11. Roles of Dgt-dependent microtubule generation in mitosis

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    Event date: 2007.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  12. Mechanisms of Microtubule Generation during Mitotic Spindle Assembly International conference

    Gordon Research Conference (Motile and Contractile Systems) 

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    Event date: 2007.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Multiple mechanisms of microtubule generation during mitotic spindle assembly were discussed.

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Research Project for Joint Research, Competitive Funding, etc. 2

  1. Plasticity of non-centrosomal microtubule networks

    2011.10 - 2014.9

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    Grant type:Competitive

  2. 細胞分裂装置が働く仕組みの研究

    2011.2 - 2014.3

    最先端・次世代研究開発プログラム 

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    Grant type:Competitive

KAKENHI (Grants-in-Aid for Scientific Research) 16

  1. 「モデル海藻」確立

    Grant number:22K19308  2022.6 - 2025.3

    科学研究費助成事業  挑戦的研究(萌芽)

    五島 剛太

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    Authorship:Principal investigator 

    Grant amount:\6370000 ( Direct Cost: \4900000 、 Indirect Cost:\1470000 )

    動物・植物・酵母の方法論を適用し、海藻の分子細胞生物学の基盤確立に挑戦する。もし研究が成功すれば、モデル動植物で日常的に行われている実験が可能になる系が初めて立ち上がることになり、波及効果は大きい。海藻の発生、細胞生理、環境応答、受精などの知見の蓄積を加速させるなど、これまでの海藻の生物学の体系を大きく変革させる潜在性を有する。また、モデル海藻と共生細菌から生理活性物質「海藻ホルモン」を見つけ出すことで創薬分野などへの貢献が見込めるなど、研究成果の多分野への波及が見込める。
    モデル動植物(線虫、シロイヌナズナ等)は、ヒトの体の発生、病気の理解や、植物育種のための基盤技術創生などに大きな貢献を果たしてきたが、海藻については、モデル実験系が存在しないことで、発生、細胞生理、環境応答、受精など、あらゆる分野において知見が決定的に欠けている。先端テクノロジーを使った育種を見据えて、海藻の生物学を理解するためには、実験系の構築は重要である。本研究では、実験モデルとなる海藻を確立することが目標である。
    モデルとしての条件として4つ考えられる。1)幅広い種に備わる特徴的な性質を有していること。2)実験室での培養が容易であること。例えば無性増殖する種は有力である。3)ライブセルイメージングや免疫染色といった細胞生物学手法が適用できること。4)分子遺伝学操作ができること。
    初年度、以下の成果が上がった。
    1) 採取した海藻のうち、緑藻・ハネモについて、微小管やアクチンの免疫染色に成功し、細胞骨格がどのように発達しているかを共焦点顕微鏡で可視化することができた。
    2)海藻にはしばしば形態形成を制御する共生細菌が存在すると考えられている。しかし、形質転換等の操作や将来のゲノム配列の決定時には、無菌培養された海藻を使用することが望ましい。そこで、抗生物質を用いた殺菌を試したところ、抗生物質存在下ではハネモの成長が著しく阻害された。共生細菌が生育に必須の役割を担っていることが示唆された。
    3)ハネモの全ゲノム配列を決定すべく、DNAを抽出しシークエンシングした(葉緑体、ミトコンドリア、核)。現在、ハネモがどういった特徴的な遺伝子を有しているか、解析を進めている。
    当初予定していた3テーマについて、いずれも進展が見られたため。
    モデルとしての条件を満たす海藻を見つけ出し基礎技術を確立するためには、
    (1)ハネモゲノムの解読、(2)必須共生細菌の同定、(3)細胞内ダイナミクスの可視化、は重要である。23年度もこの3つを柱に研究を進める。特に、ハネモゲノムのRaw dataは得られたため、これを解析すればゲノムの全貌が明らかになると期待している。

  2. 細胞分裂面決定を司る新機構の解明

    Grant number:22H02644  2022.4 - 2026.3

    科学研究費助成事業  基盤研究(B)

    五島 剛太

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    Authorship:Principal investigator 

    Grant amount:\17290000 ( Direct Cost: \13300000 、 Indirect Cost:\3990000 )

    細胞が対称あるいは非対称に分裂し、同一のあるいは互いに異なる性質を持つ娘細胞を作り出すことは、多細胞生物の発生に必要である。娘細胞の性質の差異には、細胞分裂面がどこにできるかが鍵となる。ヒメツリガネゴケの幹細胞で細胞分裂研究を展開してきた。そして最近、この系では分裂中に分裂面が決定されること強く示唆するデータを得た。本研究では、この独自に見出した分裂面決定過程がどのタンパク質のどのような働きにより駆動されるのかを明らかにする。さらに、見出した機構が他の細胞種で保存されているか、検証する。

  3. 微小管系輸送モーターの働きによる周期的な分枝形成機構

    Grant number:22H04717  2022.4 - 2024.3

    科学研究費助成事業  新学術領域研究(研究領域提案型)

    五島 剛太

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    Authorship:Principal investigator 

    Grant amount:\7800000 ( Direct Cost: \6000000 、 Indirect Cost:\1800000 )

    分枝、分岐は細胞壁を有する生物一般の代表的な成長様式の一つで、しばしば周期性を示す。本研究では、周期的な分枝形成の原理の解明を目指す。最近、微小管系輸送モーター・キネシンの1種の変異により、通常は細胞あたり一度に一つしかできない分枝が複数生じ、ヒメツリガネゴケ原糸体の分枝周期性が変調することを見出した。そこで、キネシンにより何が運ばれるかを突き止め、次の仮説を検証する。「キネシンは分枝成長に必要な物質を運び続け他の場所への物質集積を防ぐことで分枝を一箇所だけに限定させる。」
    分枝、分岐は植物に限らず細胞壁を有する生物一般の代表的な成長様式の一つであり、しばしば周期性を示す。最近、ヒメツリガネゴケの原糸体の分枝形成に周期性を持たせる鍵分子候補を見出した。それは植物に固有の微小管系輸送モーター・ARKキネシンであった。ARKの変異により通常は細胞あたり一度に一つしかできない分枝が複数生じ、原糸体の分枝周期性が変調した。この表現型は微小管上の輸送活性を失ったARKの発現ではレスキューされなかったこと、また、分枝では微小管の配向が定まっていたことから、次のような仮説が立てられた。すなわち、「周期的な分枝形成の鍵は、ARKが分枝成長に必要な物質を運び続け他の場所への物質集積を防ぐことで分枝を一箇所だけに限定させることである。」本研究ではまず、ヒメツリガネゴケでこの仮説を検証する。具体的には、ARKキネシンにより何が運ばれることが周期的な分枝形成に必要なのかを突き止める。さらに、ヒメツリガネゴケで得られた知見が他の生物種でも保存されているのかを検証する。
    <BR>
    初年度、ヒメツリガネゴケを用いた遺伝学的解析とライブ細胞観察により、ARKキネシンが多様な積荷(細胞核、葉緑体、ミトコンドリアなど)を輸送するトランスポーターであることを見出した。ARK変異体では細胞の極性化と成長に重要なアクチン制御分子RopGEF3およびRopGEF6の細胞先端蓄積に異常が生じた。さらに、RopGEF3の細胞先端への強制的な局在化によりARK変異体の成長異常は抑制された。すなわち、ARKによるアクチン制御因子輸送がヒメツリガネゴケ細胞の極性確立や成長に必要であることがわかった(Yoshida et al. in press)。
    予定していた実験を進めて、結果をまとめた論文が公表されるに至ったため。
    これまでの研究により、植物にもキネシンが複数の積荷に結合し長距離輸送する機構が存在することがわかった。また、動物や菌類ではすでに報告されていた微小管依存的輸送による細胞極性の制御が植物でも見られたことから、この機構の一般性が示唆された。一方で、植物がARKという独特なキネシンを用いる理由は未解明で、今後は他生物種での保存性にも着目した研究を展開したい。

  4. ライブ顕微イメージングを通した海生真菌類の多様性と表現型可塑性の研究

    Grant number:22H04884  2022.4 - 2024.3

    科学研究費助成事業  新学術領域研究(研究領域提案型)

    五島 剛太

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    Authorship:Principal investigator 

    Grant amount:\8320000 ( Direct Cost: \6400000 、 Indirect Cost:\1920000 )

    海には多様な真菌類(糸状菌、酵母)が生息しているが、同じ微生物のバクテリアと比べても、生態系は全く掴めていないのが現状である。さらに、最近、実験所の前の海で採集した海生酵母数種について、増殖表現型に可塑性があることを発見した。これまで実験室でプレート培養し記載されてきた成長や分裂の様式は、再検討が必要である。本研究では、海生真菌類の多様性の把握に加え、海生真菌類はどのような様式で増殖しているのかをライブ顕微鏡観察により明らかにすること、そして、表現型の可塑性の基盤となる分子機構の解明を目指す。
    海には多様な真菌類(糸状菌、酵母)が生息しているが、同じ微生物のバクテリアと比べても、生態系は全く掴めていないのが現状である。さらに最近、実験所の前の海で採集した海生酵母数種について、増殖表現型に可塑性があることを発見した。これまで実験室でプレート培養し記載されてきた成長や分裂の様式は、再検討が必要である。本研究では、海生真菌類の多様性の把握に加え、自然環境で海生真菌類は実際にどのような様式で増殖しているのかを明らかにすること、そして、表現型の可塑性の基盤となる分子機構の解明を目指す。具体的には、以下の3点が目標である。1) 実験室で実際の環境をミミックした条件を作り、ポストコッホ生態系の中での成長、分裂様式を明らかにする。2) 遺伝学解析を通じて表現型可塑性の分子基盤を明らかにする。3) 調査の手が及びにくい海域や他の生物に寄生する真菌類を採集、同定し、多様性の理解を深めるとともに、表現型可塑性の一般性を調べる。
    初年度、各地で取得した海水、泥、生物片などから真菌類のサンプリングを行った。プレート上での単離、DNAバーコード配列解析により、新たに数十種の真菌類が同定された。糸状菌と酵母が含まれていた。種が不明のものも複数取得された。ライブセルイメージングにより、細胞密度に応じて成長・分裂モードを変換する種も複数見出された。
    黒色酵母の同定不能種NU30とNU200については、培養後にDNAを抽出し、次世代シークエンサーを用いて全ゲノム配列を決定した。系統樹解析の結果、両種はDothideomycetes綱に属する未記載種であることがわかった(Kurita et al. in press)。
    期待していた通り新しい真菌類の培養に成功したことに加え、2種の全ゲノム配列を決定して論文発表が確定したため。
    (1)海生真菌類の新たな単離、(2)ポストコッホ生態系の中での成長、分裂様式の検証、(3)表現型可塑性の分子基盤の解明、を引き続き3本柱に据え、研究を継続する。特に、表現型可塑性の分子基盤については、生物進化実験を用いた変異体の取得を通じて原因遺伝子、シグナル伝達経路解明へと迫るアプローチを考えている。

  5. Study on bypass of gene essentiality

    Grant number:19K22383  2019.6 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    Goshima Gohta

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    Authorship:Principal investigator 

    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

    Although genes that control cell division are often conserved in a wide range of species, some species do not have a specific gene. In this case, it is assumed that the species has developed an alternative mechanism during the course of evolution. In this study, by means of experimental evolution, we showed such an example: a yeast strain that has lost a gene “essential” for cell division continued to proliferate by developing an alternative “masked” mechanism.

  6. Revealing the mechanism of intracellular positioning of the nucleus using cutting-edge microscopes

    Grant number:18KK0202  2018.10 - 2024.3

    Grants-in-Aid for Scientific Research  Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

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    Authorship:Coinvestigator(s) 

  7. Asymmetric cell division of plant stem cells

    Grant number:17H06471  2017.6 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Goshima Gohta

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    Authorship:Principal investigator 

    Grant amount:\152360000 ( Direct Cost: \117200000 、 Indirect Cost:\35160000 )

    Stem cell renewal and maintenance often involve asymmetric division, in which two daughter cells display different properties. However, compared to animals, the mechanism of this important process remains poorly understood in plants. In this study, we used rice, a major crop, and a model plant, the moss, to elucidate the mechanisms of a series of events during asymmetric division, namely, cell polarity establishment, mitosis, and maintenance of differentiation.

  8. Principles of pluripotent stem cells underlying plant vitality

    Grant number:17H06470  2017.6 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Umeda Masaaki

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    Authorship:Coinvestigator(s) 

    Our project aimed to understand the characteristics of plant stem cells, which support plant longevity and vitality, especially by focusing on their proliferative activity and pluripotency. To activate organic cooperation between research groups, we supported various activities including those involving animal scientists, thereby promoting collaborations. Besides, we conducted public relations activities to disseminate our research progress to Japan and abroad through international symposiums and via the homepage and newsletters.

  9. Elucidating the mechanisms of spindle formation through systematic gene deletion

    Grant number:17H01431  2017.4 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    Goshima Gohta

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    Authorship:Principal investigator 

    Grant amount:\41990000 ( Direct Cost: \32300000 、 Indirect Cost:\9690000 )

    The spindle is essential for chromosome segregation. As many genes act redundantly, the molecular mechanisms of spindle formation are not fully understood. By systematically disrupting candidate spindle regulators in model cell systems, we studied the mechanism of spindle parts formation, including the kinetochore, which is the contact point between chromosome and spindle, the ends of microtubules, which are the main components of the spindle, and the spindle pole.

  10. オーロラキナーゼシグナル伝達の数理・遺伝学的解析

    Grant number:17H06000  2017.4 - 2018.3

    新学術領域研究(研究領域提案型)

    五島 剛太

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\11700000 ( Direct Cost: \9000000 、 Indirect Cost:\2700000 )

    本研究では、細胞分裂制御の鍵キナーゼ・オーロラによるリン酸化シグナルを通じた分裂制御機構の解明を目指した。
    オーロラは細胞分裂に必須のキナーゼであり、その制御の破綻は癌化を引き起こす可能性が示唆されている。申請者らは数年前、細胞実験と数理シミュレーションにより、ヒトの細胞分裂装置・スピンドルにおいて、「オーロラキナーゼの濃度勾配が、基質である微小管脱重合酵素KIF2Aの活性勾配を規定し、これによりスピンドル長が決定される」とのシンプルなモデルを発表した (J Cell Biol. 2013)。ここでは、オーロラキナーゼが中央紡錘体微小管の先端付近に集積することで濃度勾配を形成するという知見が基となっている。
    本研究では、まず、精製した微小管、オーロラ、KIF2Aによるスピンドル長制御の試験管内再構成を通じてモデルを定性的に検証することを目指した。次に、各因子の挙動、スピンドル長変化を測定し、その定量データを数理シミュレーションに組み入れて挙動を比較し、定量的な数理モデルを完成させることを目標とした。あるいは未知の因子の存在を予言する結果が得られる可能性もあると考えた。
    研究開始後、微小管プラス端局在タンパク質EB1と融合したオーロラキナーゼの精製に成功し、試験管内でオーロラキナーゼが細胞内と同様、微小管のプラス端に集積する様子を観察した。さらに、この融合キナーゼによるKIF2Aのリン酸化にも成功した。
    研究は順調に開始されたが、重複制限により、本研究課題は廃止となった。
    29年度が最終年度であるため、記入しない。
    29年度が最終年度であるため、記入しない。

  11. 染色体分配装置の再構成

    Grant number:15KT0077  2015.7 - 2018.3

    五島 剛太

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    Authorship:Principal investigator 

    Grant amount:\18070000 ( Direct Cost: \13900000 、 Indirect Cost:\4170000 )

    キネシン8は真核生物において保存されたモータータンパク質で、動原体微小管先端に局在し動原体微小管の長さ制御および染色体整列に重要な働きをする。しかし、このタンパク質によってどのように染色体整列が制御されるかについての詳細な機構はよく分かっていない。生化学的活性についても定まっていなかった。例えばヒトのキネシン8は微小管脱重合活性があるとする報告と、微小管伸縮を抑制するという報告がある。
    本研究では前年度までに、ショウジョウバエにひとつだけ存在するキネシン8の生化学的活性を決定した。すなわち、キネシン8タンパク質全長を精製し、試験管内において伸び縮みする動的な微小管と反応させると、微小管の伸長から短縮に移行する「カタストロフ」と呼ばれる現象の頻度が上昇し、微小管の長さを制限した。さらに精製したキネシン8は微小管の短縮速度を減少させ、伸長、短縮いずれも起こらない、「ポーズ」と呼ばれる現象や、短縮から伸長に移行する「レスキュー」と呼ばれる現象の頻度を上昇させた。
    今年度は、これらの活性がキネシン8の動原体機能にどう関与するかを明らかにすることを目指した。ショウジョウバエS2細胞においてキネシン8を欠損させると異常に長い動原体微小管が観察されるが、S2細胞においてキネシン8を欠損させ、さらに微小管重合阻害剤であるコルセミドを加え微小管の長さを制限したが、染色体整列異常の表現型をレスキューできなかった。このことから、キネシン8のカタストロフを促進する以外の機能も染色体整列に重要なことが示唆された。そこで、ショウジョウバエS2細胞においてキネシン8を欠損させ、動原体-微小管の結合を詳細に観察することにした。
    研究は順調に進んだが、重複制限により、本研究課題は廃止となった。

  12. Searching for the "plant dynein"

    Grant number:15K14540  2015.4 - 2018.3

    Goshima Gohta

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\3900000 ( Direct Cost: \3000000 、 Indirect Cost:\900000 )

    In animal cells, the retrograde transport of intracellular cargo along microtubules is executed by the cytoplasmic dynein motor (retrograde transport is transport towards the minus end of the microtubule). Interestingly, however, land plants have lost the cytoplasmic dynein gene, despite that they execute retrograde transport. In this research, we aim to identify the motor(s) responsible for retrograde transport in plants. Using he moss Physcomitrella patens, we identified three kinesin-14 motor proteins (KCBP, KCH, ATK) that were required for retrograde transport of the nucleus, chloroplast, and microtubule itself in the moss cytoplasm.

  13. Microtubule generation independent of centrosomes

    Grant number:26711012  2014.4 - 2018.3

    Goshima Gohta

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    Authorship:Principal investigator 

    Grant amount:\24050000 ( Direct Cost: \18500000 、 Indirect Cost:\5550000 )

    This research project aimed to understand the mechanism of centrosome-independent microtubule generation. The major outcomes are as follows: (1) The augmin complex has been shown required for centrosome-independent microtubule nucleation within the mitotic spindle in animal and plant cells. Here, we identified augmin in the filamentous fungus, elucidating the evolutionary conservation of this protein complex. (2) We generated knockout mice of a critical augmin subunit, and identified a defect in MTOC clustering during early embryonic division. (3) Microtubule nucleation independent of centrosomes or augmin was identified in the moss Physcomitrella patens.

  14. 中心体に依存しない微小管生成機構

    2014.4 - 2017.3

    科学研究費補助金  若手研究(A)

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    Authorship:Principal investigator 

  15. 細胞内における微小管生成機構とその役割の解明

    2008.4 - 2011.3

    科学研究費補助金  若手研究(A),課題番号:20687013

    五島 剛太

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    Authorship:Principal investigator 

  16. 動原体の核内配置機構とその役割の解明                      

    2008.4 - 2009.3

    科学研究費補助金  萌芽研究,課題番号:20657002

    五島 剛太

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    Authorship:Principal investigator 

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