研究者詳細 - 加藤　竜司

論文 - 加藤　竜司

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Importance of dataset design in developing robust U-Net models for label-free cell morphology evaluation.

Shiina T, Kimura K, Takemoto Y, Tanaka K, Kato R

Journal of bioscience and bioengineering 139 巻 ( 4 ) 頁： 329 - 339 2025年4月

記述言語：英語出版者・発行元：Journal of Bioscience and Bioengineering

Advances in regenerative medicine highlighted the need for label-free cell image analysis to replace conventional microscopic observation for non-invasive cell quality evaluation. Image-based evaluation provides an efficient, quantitative, and automated approach to cell analysis, but segmentation remains a critical and challenging step. In this study, we investigated how training dataset design influenced the robustness of U-Net models for cell segmentation, focusing on challenges posed by limited data availability in cell culture. Using 2592 image pairs from four cell types representing key morphological categories, we constructed 42 investigation patterns to evaluate the effects of dataset size, dataset content, and morphological diversity on model performance. Our results showed that robust segmentation models could be developed with approximately 10 raw images captured using a 4× objective lens, a much smaller dataset than typically assumed. The dataset content was found to be crucial: training dataset images that captured commonly observed cell patterns yielded more robust models compared to those capturing rare or irregular cell patterns, which often impaired model performance with large deviations. Additionally, including both spindle and round cell morphologies in the training datasets improved model robustness when tested across all four cell types, while datasets restricted to a single morphology type could not achieve robust models. These findings highlight the importance of curating datasets that capture representative yet diverse cell morphologies. By addressing critical questions about dataset design, this study provides actionable guidance for the effective use of deep learning-based cell segmentation models in manufacturing and research applications.

DOI： 10.1016/j.jbiosc.2025.01.004

Scopus

PubMed

Medicinal cannabis extracts are neuroprotective against Aβ1-42-mediated toxicity in vitro Open Access

Marsh, DT; Shibuta, M; Kato, R; Smid, SD

BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY 135 巻 ( 5 ) 頁： 575 - 592 2024年11月

記述言語：英語出版者・発行元：Basic and Clinical Pharmacology and Toxicology

Background: Phytocannabinoids inhibit the aggregation and neurotoxicity of the neurotoxic Alzheimer's disease protein β amyloid (Aβ). We characterised the capacity of five proprietary medical cannabis extracts, heated and non-heated, with varying ratios of cannabidiol and Δ9-tetrahydrocannabinol and their parent carboxylated compounds to protect against lipid peroxidation and Aβ-evoked neurotoxicity in PC12 cells. Methods: Neuroprotection against lipid peroxidation and Aβ1–42-induced cytotoxicity was assessed using the thiazolyl blue tetrazolium bromide (MTT) assay. Transmission electron microscopy was used to visualise phytocannabinoid effects on Aβ1–42 aggregation and fluorescence microscopy. Results: Tetrahydrocannabinol (THC)/tetrahydrocannabinolic acid (THCA)-predominant cannabis extracts demonstrated the most significant overall neuroprotection against Aβ1–42-induced loss of PC12 cell viability. These protective effects were still significant after heating of extracts, while none of the extracts provided significant neuroprotection to lipid peroxidation via tbhp exposure. Modest inhibition of Aβ1–42 aggregation was demonstrated only with the non-heated BC-401 cannabis extract, but overall, there was no clear correlation between effects on fibrils and conferral of neuroprotection. Conclusions: These findings highlight the variable neuroprotective activity of cannabis extracts containing major phytocannabinoids THC/THCA and cannabidiol (CBD)/cannabidiolic acid (CBDA) on Aβ-evoked neurotoxicity and inhibition of amyloid β aggregation. This may inform the future use of medicinal cannabis formulations in the treatment of Alzheimer's disease and dementia.

DOI： 10.1111/bcpt.14078

Editorial: Artificial intelligence in predicting, determining and controlling cell phenotype or tissue function in inflammatory diseases Open Access

Hart, ML; Kato, R; Rolauffs, B

FRONTIERS IN IMMUNOLOGY 15 巻頁： 1443534 2024年6月

Construction of transcript regulation mechanism prediction models based on binding motif environment of transcription factor AoXlnR in Aspergillus oryzae

Oka, H; Kojima, T; Kato, R; Ihara, K; Nakano, H

JOURNAL OF BIOINFORMATICS AND COMPUTATIONAL BIOLOGY 22 巻 ( 03 ) 頁： 2450017 2024年6月

記述言語：英語出版者・発行元：Journal of Bioinformatics and Computational Biology

DNA-binding transcription factors (TFs) play a central role in transcriptional regulation mechanisms, mainly through their specific binding to target sites on the genome and regulation of the expression of downstream genes. Therefore, a comprehensive analysis of the function of these TFs will lead to the understanding of various biological mechanisms. However, the functions of TFs in vivo are diverse and complicated, and the identified binding sites on the genome are not necessarily involved in the regulation of downstream gene expression. In this study, we investigated whether DNA structural information around the binding site of TFs can be used to predict the involvement of the binding site in the regulation of the expression of genes located downstream of the binding site. Specifically, we calculated the structural parameters based on the DNA shape around the DNA binding motif located upstream of the gene whose expression is directly regulated by one TF AoXlnR from Aspergillus oryzae, and showed that the presence or absence of expression regulation can be predicted from the sequence information with high accuracy (AUPRC=0.8-1.0) by machine learning incorporating these parameters.

DOI： 10.1142/S0219720024500173

Web of Science

Scopus

PubMed

Image-based hybrid model incorporating initial spatial distribution for mesenchymal stem cell cultivation process design

Hirono, K; Hayashi, Y; Udugama, IA; Takemoto, Y; Kato, R; Kino-oka, M; Sugiyama, H

AICHE JOURNAL 2024年4月

出版者・発行元：AIChE Journal

This work presents an image-based hybrid model incorporating the initial spatial distribution for mesenchymal stem cell (MSC) cultivation process design. First, three levels of seeding bias in static MSC cultivation were experimentally investigated, and the resulting initial distribution was quantified using phase contrast microscopy image analysis. Second, the observed spatial heterogeneity was defined as a new parameter by calculating the standard deviation of the seeded cell number fraction among numerical 8 × 8 tiles on two-dimensional plates. Third, the parameter was incorporated into kinetic models to consider spatial growth limitation. The model was then applied to simulate MSC cultivation processes with experimental data. Using dynamic and stochastic simulation outputs, feasible ranges of cell-harvesting time could be determined to satisfy a given requirement for the minimum cell number and acceptable confluency level. The developed hybrid model could serve as a basis for quantitative decision-making in the design of MSC cultivation processes.

DOI： 10.1002/aic.18452

Web of Science

Scopus

Prediction of antibody production performance change in Chinese hamster ovary cells using morphological profiling. 査読有り

Hisada T, Imai Y, Takemoto Y, Kanie K, Kato R

Journal of bioscience and bioengineering 2024年3月

Development of novel waxy bone haemostatic agents composed of biodegradable polymers with osteogenic-enhancing peptides in rabbit models 査読有り

Ohno, T; Suenaga, H; Yamawaki-Ogata, A; Kanie, K; Kato, R; Uto, K; Ebara, M; Ito, H; Narita, Y; Usui, A; Mutsuga, M

Interdisciplinary cardiovascular and thoracic surgery 37 巻 ( 5 ) 2023年11月

The structurally diverse phytocannabinoids cannabichromene, cannabigerol and cannabinol significantly inhibit amyloid β-evoked neurotoxicity and changes in cell morphology in PC12 cells. 査読有り国際共著

Marsh DT, Sugiyama A, Imai Y, Kato R, Smid SD

Basic & clinical pharmacology & toxicology 2023年9月

Automated cell isolation from photodegradable hydrogel based on fluorescence image analysis. 査読有り

Shinji Sugiura, Shinya Yamahira, Masato Tamura, Kazumi Shin, Mayu Shibuta, Taku Satoh, Yui Matsuzawa, Gen Fujii, Fumiki Yanagawa, Michihiro Mutoh, Masumi Yanagisawa, Ryuji Kato, Hirofumi Matsui

BIOTECHNOLOGY AND BIOENGINEERING 2023年3月

Novel functional peptide for next-generation vital pulp therapy 査読有り

Watanabe Masakatsu, Okamoto Motoki, Komichi Shun, Huang Hailing, Matsumoto Sayoko, Moriyama Kiichi, Ohshima Jun, Abe Shotaro, Morita Masayoshi, Ali Manahil,, Takebe Katsuki, Kozaki Ikko, Fujimoto Akiyo, Kanie Kei, Kato Ryuji, Uto Kenichiro, Ebara Mitsuhiro, Yamawaki-Ogata Aika, Narita Yuji, Takahashi Yusuke, Hayashi Mikako

JOURNAL OF DENTAL RESEARCH 102 巻 ( 3 ) 頁： 322 - 330 2023年3月

Bioinformatics in bioscience and bioengineering: Recent advances, applications, and perspectives 査読有り

Uesaka Kazuma, Oka Hiroya, Kato Ryuji, Kanie Kei, Kojima Takaaki, Tsugawa Hiroshi, Toda Yosuke, Horinouchi Takaaki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 134 巻 ( 5 ) 頁： 363 - 373 2022年11月

天野日出, 木村和恵, 今井佑太, 加藤竜司, 中澤温子, 城田千代栄, 滝本愛太朗, 住田亙, 牧田智, 岡本眞宗, 安井昭洋, 高田瞬也, 中川洋一, 加藤大幾, 檜顕成, 内田広夫

小児外科 54 巻 ( 10 ) 頁： 1007 - 1011 2022年10月

Morphology-based noninvasive early prediction of serial-passage potency enhances the selection of clone-derived high-potency cell bank from mesenchymal stem cells 査読有り

Suyama Takashi, Takemoto Yuto, Miyauchi Hiromi, Kato Yuko, Matsuzaki Yumi, Kato Ryuji

INFLAMMATION AND REGENERATION 42 巻 ( 1 ) 頁： 30 2022年10月

Label-free morphological sub-population cytometry for sensitive phenotypic screening of heterogenous neural disease model cells 査読有り

Imai Yuta, Iida Madoka, Kanie Kei, Katsuno Masahisa, Kato Ryuji

SCIENTIFIC REPORTS 12 巻 ( 1 ) 頁： 9296 2022年6月

Bi-layered carboxymethyl cellulose-collagen vitrigel dual-surface adhesion-prevention membrane 査読有り

Wang Yue, Kanie Kei, Takezawa Toshiaki, Horikawa Miki, Kaneko Kyoshiro, Sugimoto Ayako, Yamawaki-Ogata Aika, Narita Yuji, Kato Ryuji

CARBOHYDRATE POLYMERS 285 巻頁： 119223 2022年6月

Myc supports self-renewal of basal cells in the esophageal epithelium. 査読有り国際共著

Tomoaki Hishida, Eric Vazquez-Ferrer, Yuriko Hishida-Nozaki, Yuto Takemoto, Fumiyuki Hatanaka, Kei Yoshida, Javier Prieto, Sanjeeb Kumar Sahu, Yuta Takahashi, Pradeep Reddy, David D O'Keefe, Concepcion Rodriguez Esteban, Paul S Knoepfler, Estrella Nuñez Delicado, Antoni Castells, Josep M Campistol, Ryuji Kato, Hiroshi Nakagawa, Juan Carlos Izpisua Belmonte

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 10 巻頁： 786031 2022年3月

Collection of Data Variation Using a High-Throughput Image-Based Assay Platform Facilitates Data-Driven Understanding of TRPA1 Agonist Diversity 査読有り

Terada Yuko, Tanaka Kenjiro, Matsuyama Minami, Fujitani Masaya, Shibuya Masatoshi, Yamamoto Yoshihiko, Kato Ryuji, Ito Keisuke

APPLIED SCIENCES 12 巻 ( 3 ) 2022年2月

Morphological heterogeneity description enabled early and parallel non-invasive prediction of T-cell proliferation inhibitory potency and growth rate for facilitating donor selection of human mesenchymal stem cells 査読有り

Imai Yuta, Kanie Kei, Kato Ryuji

INFLAMMATION AND REGENERATION 42 巻 ( 1 ) 頁： 8 2022年1月

Enhancement of protein thermostability by three consecutive mutations using loop-walking method and machine learning 査読有り

Yoshida Kazunori, Kawai Shun, Fujitani Masaya, Koikeda Satoshi, Kato Ryuji, Ema Tadashi

SCIENTIFIC REPORTS 11 巻 ( 1 ) 頁： 11883 2021年6月

Predicting quality decay in continuously passaged mesenchymal stem cells by detecting morphological anomalies.  査読有り

Takemoto Yuto, Imai Yuta, Kanie Kei, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 131 巻 ( 2 ) 頁： 198 - 206 2021年2月

機械学習を用いた浸炭熱処理品のミクロ組織画像からの炭素濃度予測技術の開発査読有り

谷地宣紀, 服部清幸, 加藤竜司

熱処理 61 巻 ( 5 ) 頁： 257 - 263 2021年

記述言語：日本語出版者・発行元：一般社団法人日本熱処理技術協会

浸炭焼入焼戻し処理は表面硬化熱処理として広く使われており炭素濃度によって性能が変化することが知られている。筆者らは近年着目されている画像認識技術と機械学習を用いて浸炭焼入焼戻し後のミクロ組織から炭素濃度を予測する技術を開発した。この技術は画像の黒色部と白色部を抽出した後，それぞれの形状情報と輝度情報を特徴量として機械学習させる手法である。この技術を基盤としてミクロ組織の性状に応じた回帰モデルを用いることによって0.78 mass％のミクロ組織を0.75 mass％から0.79 mass％の範囲で予測させることに成功した。

DOI： 10.14940/netsushori.61.257

CiNii Research

身近となった画像解析とAI

加藤竜司

生物工学会誌 99 巻 ( 8 ) 頁： 432 - 435 2021年

Screening of FFAR1-activating peptides by molecular structural analysis 査読有り

Yoshioka Keitaro, Yamashita Haruki, Fujitani Masaya, Kato Ryuji, Shimizu Kazunori, Honda Hiroyuki

KAGAKU KOGAKU RONBUNSHU 47 巻 ( 3 ) 頁： 64 - 68 2021年

記述言語：日本語出版者・発行元：The Society of Chemical Engineers, Japan

To find FFAR1-activating peptides consisting of natural amino acid residues, molecular structure analysis was performed by hierarchical clustering using extraction of physicochemical parameters. When 3 mer to 6 mer peptide libraries were prepared and the molecular structure of each was compared with 5 chemical agonists, 12 peptides with high correlation coefficients of physicochemical parameters were found from the 6 mer peptide library. These peptides were then synthesized by Fmoc solid phase synthesis, and their activity was assessed by TGFα shedding assay using FFAR1-expressing HEK293. As a result, two peptides, GCGGSS and GASGCC, were identified as peptide agonists with relatively high activity. Although these peptides showed approximately one fifth of the activity of chemical agonist GW9508, they are the first examples that we know of FFAR1-activating peptides consisting of natural amino acid residues.

DOI： 10.1252/kakoronbunshu.47.64

Web of Science

Scopus

Machine learning-based amino-acid substitution of short peptides: Acquisition of peptides with enhanced inhibitory activities against α-amylase and α-glucosidase. 査読有り

Yamashita Haruki, Fujitani Masaya, Shimizu Kazunori, Kanie Kei, Kato Ryuji, Honda Hiroyuki

ACS BIOMATERIALS SCIENCE & ENGINEERING 6 巻 ( 11 ) 頁： 6117 - 6125 2020年11月

In vitro model of human skeletal muscle tissues with contractility fabricated by immortalized human myogenic cells. 査読有り

Nagashima Takunori, Hadiwidjaja Stacy, Ohsumi Saki, Murata Akari, Hisada Takumi, Kato Ryuji, Okada Yohei, Honda Hiroyuki, Shimizu Kazunori

ADVANCED BIOSYSTEMS 4 巻 ( 11 ) 頁： e2000121 2020年11月

Reproducible production and image-based quality evaluation of retinalpigment epithelium sheets from human induced pluripotent stem cells. 査読有り

Ye Ke, Takemoto Yuto, Ito Arisa, Onda Masanari, Morimoto Nao, Mandai Michiko, Takahashi Masayo, Kato Ryuji, Osakada Fumitaka

SCIENTIFIC REPORTS 10 巻 ( 1 ) 頁： 14387 2020年9月

The importance of scoring recognition fitness in spheroid morphological analysis for robust label -free quality evaluation 査読有り

Shirai Kazuhide, Kato Hirohito, Imai Yuta, Shibuta Mayu, Kanie Kei, Kato Ryuji

REGENERATIVE THERAPY 14 巻頁： 205 - 214 2020年6月

Performance of a biodegradable compositewith hydroxyapatite as a scaffold in pulp tissue repair. 査読有り

Okamoto Motoki, Matsumoto Sayako, Sugiyama Ayato, Kanie Kei, Watanabe Masakatsu, Huang Hailing, Ali Manahil, Ito Yuki, Miura Jiro, Hirose Yujiro, Uto Koichiro, Ebara Mitsuhiro, Kato Ryuji, Yamawaki-Ogata Aika, Narita Yuji, Kawabata Shigetada, Takahashi Yusuke, Hayashi Mikako

POLYMERS (BASEL) 12 巻 ( 4 ) 頁： 937 - 941 2020年4月

Novel cannabis flavonoid, cannflavin A displays both a hormetic and neuroprotective profile against amyloid β-mediatedneurotoxicity in PC12 cells: Comparison with geranylated flavonoids, mimuloneand diplacone. 査読有り国際共著

Eggers Carly, Fujitani Masaya, Kato Ryuji, Smid Scott

BIOCHEMICAL PHARMACOLOGY 169 巻頁： 113609 2019年8月

Effect of mechanical vibration stress in cell culture on human inducedpluripotent stem cells. 査読有り

Kanie Kei, Sakai Teppei, Imai Yuta, Yoshida Kei, Sugimoto Ayako, Makino Hodaka, Kubo Hirotsugu, Kato Ryuji

REGENERATIVE THERAPY 12 巻頁： 27 - 35 2019年6月

Quantitative analysisof operators' flow line in the cell culture for controlled manual operation. 査読有り

Kanie Kei, Sasaki Hiroto, Ikeda Yurika, Tamada Masaki, Togawa Fumio, Kato Ryuji

REGENERATIVE THERAPY 12 巻頁： 43 - 54 2019年5月

Identification of an early cell fate regulator bydetecting dynamics in transcriptional heterogeneity and co-regulation duringastrocyte differentiation. 査読有り

Ando Tatsuya, Kato Ryuji, Honda Hiroyuki

NPJ SYSTEMS BIOLOGY AND APPLICATIONS 5 巻 ( 1 ) 頁： 18 2019年5月

Numerical investigation of particle dispersion in the preprocessing stage for a staticcell cultivation. 査読有り

Sekimoto Atsushi, Kanemaru Yoshiki, Okano Yasunori, Kanie Kei, Kato Ryuji, Kino-oka Masahiro

REGENERATIVE THERAPY 12 巻 ( 83 ) 頁： 87 2019年4月

Activation of cancer-related and mitogen-activated protein kinase signaling pathways in human mature osteoblasts isolated from patients with type 2 diabetes. 査読有り

Kuroiwa Tomoyuki, Matsumoto Megumi, Kato Ryuji, Nimura Akimoto, Yoshii Toshitaka, Okawa Atsushi, Fujita Koji

BONE REPORTS 10 巻頁： 100199 2019年2月

Time-course colony tracking analysis for evaluating induced pluripotent stem cellculture processes. 査読有り

Yoshida Kei, Okada Mai, Nagasaka Risako, Sasaki Hiroto, Kanie Kei, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 128 巻 ( 2 ) 頁： 209 - 217 2019年2月

Morphology-based analysis of myoblasts for prediction of myotube formation. 査読有り

Ishikawa Kiyoshi, Yoshida Kei, Kanie Kei, Omori Kenji, Kato Ryuji

SLAS DISCOVERY 24 巻 ( 1 ) 頁： 47 - 56 2019年1月

細胞形態情報解析を用いた 1 細胞～細胞集団の応答性の解析

加藤竜司

日本プロテオーム学会大会要旨集 2019 巻 ( 0 ) 頁： 131 - 131 2019年

In-process evaluation of culture errors using morphology-based image analysis. 査読有り

Imai Yuta, Yoshida Kei, Matsumoto Megumi, Okada Mai, Kanie Kei, Shimizu Kazunori, Honda Hiroyuki, Kato Ryuji

REGENERATIVE THERAPY 9 巻 ( 15 ) 頁： 15 - 23 2018年12月

Effective screening for hypolipidemic bile acid-binding peptides by hierarchical clustering-based catalog array. 査読有り

Kato Ryuji, Kanie Kei, Kaga Chiaki, Okochi Mina, Nagaoka Satoshi, Honda Hiroyuki

JOURNAL OF BIOSCIENCE BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 1 巻 ( 1 ) 頁： doi: 10.31546/BBAB.1001 2018年8月

Imaging cell picker: A morphology-based automatedcell separation system on a photodegradable hydrogel culture platform. 査読有り

Mayu Shibuta, Masato Tamura, Kei Kanie, Masumi Yanagisawa, Hirofumi Matsui, Taku Satoh, Toshiyuki Takagi, Toshiyuki Kanamori, Shinji Sugiura, Ryuji Kato

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 126 巻 ( 5 ) 頁： 653 - 660 2018年6月

画像情報処理を用いた再生医療用製品製造工程における非破壊的品質管理技術の開発

加藤竜司

生物工学会誌 96 巻 ( 3 ) 頁： 121 - 128 2018年

細胞画像情報処理を用いた医療用細胞製造プロセス管理

加藤竜司

精密工学会誌 84 巻 ( 11 ) 頁： 901 - 904 2018年

Morphology-based non-invasive quantitative prediction of the differentiationstatus of neural stem cells. 査読有り

Fujitani Masaya, Huddin Noor Safika, Kawai Shun, Kanie Kei, Kiyota Yasujiro, Shimizu Kazunori, Honda Hiroyuki, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 124 巻 ( 3 ) 頁： 351 - 358 2017年9月

Heat-stimuli-enhanced osteogenesis using clinically available biomaterials. 査読有り

Ota Takehiro, Nishida Yoshihiro, Ikuta Kunihiro, Kato Ryuji, Kozawa Eiji, Hamada Shunsuke, Sakai Tomohisa, Ishiguro Naoki

PLOS ONE 12 巻 ( 7 ) 頁： e0181404 2017年7月

Visualization of morphological categories of colonies formonitoring of effect on induced pluripotent stem cell culture status. 査読有り

Nagasaka Risako, Matsumoto Megumi, Okada Mai, Sasaki Hiroto, Kanie Kei, Kii Hiroaki, Uozumi Takayuki, Kiyota Yasujiro, Honda Hiroyuki, Kato Ryuji

REGENERATIVE THERAPY 6 巻頁： 41 - 51 2017年6月

Image-based cell quality evaluation to detect irregularities under same cultureprocess of human induced pluripotent stem cells. 査読有り

Nagasaka Risako, Gotou Yuto, Yoshida Kei, Kanie Kei, Shimizu Kazunori, Honda Hiroyuki, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 123 巻 ( 5 ) 頁： 642 - 650 2017年5月

Prediction of differentiation tendency toward hepatocytes from gene expression in undifferentiated human pluripotent stem cells. 査読有り

Yanagihara Kana, Liu Yujung, Kanie Kei, Takayama Kazuo, Kokunugi Minako, Hirata Mitsuhi, Fukuda Takayuki, Suga Mika, Nikawa Hiroki, Mizuguchi Hiroyuki, Kato Ryuji, Furue-Kusuda Miho

STEM CELLS AND DEVELOPMENT 25 巻 ( 24 ) 頁： 1884 - 1897 2016年12月

Focused screening of ECM-selective adhesion peptides on cellulose-bound peptide microarrays. 査読有り

Kanie Kei, Kondo Yuto, Owaki Junki, Ikeda Yurika, Narita Yuji, Kato Ryuji, Honda Hiroyuki

BIOENGINEERING (BASEL) 3 巻 ( 4 ) 頁： 31 2016年11月

Combinational effects of polymer viscoelasticity and immobilized peptides on cell adhesion to cell-selective scaffolds. 査読有り

Kurimoto Rio, Kanie Kei, Uto Koichiro, Kawai Shun, Hara Mitsuo, Nagano Shusaku, Narita Yuji, Honda Hiroyuki, Naito Masanobu, Ebara Mitsuhiro, Kato Ryuji

ANALYTICAL SCIENCES 32 巻 ( 11 ) 頁： 1195 - 1202 2016年11月

Parametric analysis of colony morphology of non-labelled live human pluripotent stem cells for cell quality control. 査読有り

Kato Ryuji, Matsumoto Megumi, Sasaki Hiroto, Joto Risako, Okada Mai, Ikeda Yurika, Kanie Kei, Suga Mika, Kinehara Masaki, Yanagihara Kana, Liu Yujung, Uchio-Yamada Kozue, Fukuda Takayuki, Kii Hiroaki, Uozumi Takayuki, Honda Hiroyuki, Kiyota Yasujiro, Furue-Kusuda Miho

SCIENTIFIC REPORTS 6 巻頁： 34009 2016年9月

Exploring high-affinity binding properties of octamer peptides by principal component analysisof tetramer peptides. 査読有り

Kume Akiko, Kawai Shun, Kato Ryuji, Iwata Shinmei, Shimizu Kazunori, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 123 巻 ( 2 ) 頁： 230 - 238 2016年9月

Screeningof osteogenic-enhancing short peptides from BMPs for biomimetic material applications. 査読有り

Kanie Kei, Kurimoto Rio, Tian Jing, Ebisawa Katsumi, Narita Yuji, Honda Hiroyuki, Kato Ryuji

MATERIALS (BASEL) 9 巻 ( 9 ) 頁： 730 2016年9月

Morphological evaluation of nonlabeled cells to detect stimulation of nerve growth factor expression by Lyconadin B. 査読有り

Kawai Shun, Sasaki Hiroto, Okada Norihiro, Kanie Kei, Yokoshima Satoshi, Fukuyama Tohru, Honda Hiroyuki, Kato Ryuji

JOURNAL OF BIOMOLECULAR SCREENING 21 巻 ( 8 ) 頁： 795 - 803 2016年9月

Non-invasive quality evaluation of confluent cells by image-based orientation heterogeneity analysis 査読有り

Sasaki Kei, Sasaki Hiroto, Takahashi Atsuki, Kang Siu, Yuasa Tetsuya, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 121 巻 ( 2 ) 頁： 227 - 234 2016年2月

Comparisons of cellculture medium using distribution of morphological features in microdevice. 査読有り

Sasaki Hiroto, Enomoto Junko, Ikeda Yurika, Honda Hiroyuki, Fukuda Junji, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 121 巻 ( 1 ) 頁： 117 - 123 2016年1月

Combinational effect of cell adhesion biomolecules and their immobilized polymer property to enhance cell-selective adhesion. 査読有り

Kurimoto Rio, Kei Kanie, Koichiro Uto, Shun Kawai, Mitsuo Hara, Shusaku Nagano, Yuji Narita, Hiroyuki Honda, Masanobu Naito, Mitsuhiro Ebara, Ryuji Kato

INTERNATIONAL JOURNAL OF POLYMER SCIENCE 2016 巻頁： 2090985 2016年

Combinational effects of polymer viscoelasticity and immobilized peptides on cell adhesion to cell-selective scaffolds. 査読有り

Kurimoto Rio, Kei Kanie, Koichiro Uto, Shun Kawai, Mitsuo Hara, Shusaku Nagano, Yuji Narita, Hiroyuki Honda, Masanobu Naito, Mitsuhiro Ebara, Ryuji Kato

ANALYTICAL SCIENCES 32 巻 ( 11 ) 頁： 1195 - 1202 2016年

Differential variability and correlation of geneexpression identifies key genes involved in neuronal differentiation. 査読有り

Ando Tatsuya, Kato Ryuji, Honda Hiroyuki

BMC SYSTEMS BIOLOGY 9 巻 ( 1 ) 頁： 82 2015年11月

Image-based focusedcounting of dividing cells for non-invasive monitoring of regenerative medicineproducts. 査読有り

Sasaki K, Miyata H, Sasaki H, Kang S, Yuasa T, Kato R.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 120 巻 ( 5 ) 頁： 582 - 590 2015年11月

Image-based focused counting of dividing cells for non-invasive monitoring of regenerative medicine products 査読有り

Sasaki Kei, Miyata Hirofumi, Sasaki Hiroto, Kang Siu, Yuasa Tetsuya, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 120 巻 ( 5 ) 頁： 582 - 590 2015年11月

Effects of theproperties of short peptides conjugated with cell-penetrating peptides on theirinternalization into cells. 査読有り

Matsumoto Ryo, Okochi Mina, Shimizu Kazunori, Kanie Kei, Kato Ryuji, Honda Hiroyuki

SCIENTIFIC REPORTS 5 巻頁： 12884 2015年8月

配向性解析によるコンフレント細胞の非侵襲品質評価 (医用画像)

佐々木啓, 佐々木寛人, 高橋厚妃, 蟹江慧, 姜時友, 加藤竜司, 湯浅哲也

電子情報通信学会技術研究報告 114 巻 ( 482 ) 頁： 167 - 172 2015年3月

出版者・発行元：一般社団法人電子情報通信学会

近年,再生医療は実用化に向けて急速に発展しつつあるが,その実現に対する技術的障壁の一つに非侵襲的な細胞品質評価の難しさがある.この問題に対して,非侵襲的に取得される位相差顕微鏡画像から細胞品質を定量評価する画像処理・情報解析技術が注目されている.しかしながら,コンフレントな状態の細胞画像は,形態的特徴量が乏しいために細胞の正確な認識が技術的に難しいという課題があった.本研究では,コンフレントな細胞画像に現れる細胞の集団的な情報をスコア化する画像処理技術として,細胞画像の配向性・ヘテロ性に着目し,それらから特徴量を抽出する手法を考案する.実験データに関してコンフレント細胞の状態を判別することで,提案手法の有効性を定量的に評価する.

Identification of an interactionbetween VWF rs7965413 and platelet count as a novel risk marker for metabolicsyndrome an extensive search of candidate polymorphisms in a case-controlstudy. 査読有り

Nakatochi Masahiro, Ushida Yasunori, Yasuda Yoshinari, Yoshida Yasuko, Kawai Shun, Kato Ryuji, Nakashima Toru, Iwata Masamitsu, Kuwatsuka Yachiyo, Ando Masahiko, Hamajima Nobuyuki, Kondo Takaaki, Oda Hiroaki, Hayashi Mutsuharu, Kato Sawako, Yamaguchi Makoto, Maruyama Shoichi, Matsuo Seiichi, Honda Hiroyuki

PLOS ONE 10 巻 ( 2 ) 頁： e0117591 2015年2月

In vivo heat-stimulus-triggered osteogenesis. 査読有り

Ikuta Kunihiro, Urakawa Hiroshi, Kozawa Eiji, Hamada Shunsuke, Ota Takehiro, Kato Ryuji, Honda Hiroyuki, Kobayashi Takeshi, Ishiguro Naoki, Nishida Yoshihiro

INTERNATIONAL JOURNAL OF HYPERTHERMIA 31 巻 ( 1 ) 頁： 58 - 66 2015年2月

Characterization of time-course morphological features for efficientprediction of osteogenic potential in human mesenchymal stem cells. 査読有り

Matsuoka Fumiko, Takeuchi Ichiro, Agata Hideki, Kagami Hideaki, Shiono Hirofumi, Kiyota Yasujiro, Honda Hiroyuki, Kato Ryuji

BIOTECHNOLOGY AND BIOENGINEERING 111 巻 ( 7 ) 頁： 1430 - 1439 2014年7月

Label-free morphology-based prediction of multiple differentiation potentials ofhuman mesenchymal stem cells for early evaluation of intact cells. 査読有り

Sasaki Hiroto, Takeuchi Ichiro, Okada Mai, Sawada Rumi, Kanie Kei, Kiyota Yasujiro, Honda Hiroyuki, Kato Ryuji

PLOS ONE 9 巻 ( 4 ) 頁： e93952 2014年4月

ペプチドアレイを用いたECMタンパク質由来機能性ペプチドの探索ストラテジーとその可能性

蟹江慧, 加藤竜司

化学と生物 52 巻 ( 6 ) 頁： 361 - 370 2014年

担当区分：最終著者,　責任著者出版者・発行元：公益社団法人日本農芸化学会

生物，特にヒトのような高等動物は体の仕組みが巧妙に整理されている．体は，構成する単位の小さいものから細胞，組織，臓器，器官と構成され，臓器一つをとってみても複雑ではあるが，組織という構成単位で美しく整列している．最近話題となっている再生医療は最小単位である細胞を用い，組織や臓器を作製することが最終目的であり，構成単位が乱れると患者に重篤な副作用を及ぼす．再生医療の考えの一つに組織工学があり，失った組織を細胞を用いることで構成させ，欠損組織を代替・再生させる．この考えのもと人工血管・人工心臓・人工骨・人工歯などの体内留置型医療機器が幅広く開発されてきている．しかし，これらの医療機器は再生というよりは代替の意味合いが強く，完全再生に至っていないため副作用を及ぼす．筆者らは，再生の答えが生体内に隠されており，生体を学ぶことにより解決策を生み出すのではないかと考えた．生体内には細胞の挙動を制御する分子として細胞外マトリックス (ECM:Extracellular matrix) が存在している．その中でもECMタンパク質をターゲットとし，ECMタンパク質を構成する小成分としてペプチドに着目した．本稿では，ペプチドアレイという機能性ペプチド探索ツールを用い，ある細胞には接着するが，ある細胞には接着しない細胞選択的に接着するペプチドを探索するいくつかの手法を紹介する．そして，ペプチドアレイを使用した探索手法を通し，ECM中に存在するペプチドと細胞選択性に関して考察し，ペプチドアレイ探索による生命現象の解釈と再生促進型医療機器開発の可能性に関して述べる．

DOI： 10.1271/kagakutoseibutsu.52.361

培養中の幹細胞品質評価 : 画像を用いた評価技術とその貢献 (特集再生医療実現に向けた幹細胞培養工学の最前線)

加藤竜司, 清田泰次郎, 備瀬竜馬

生物工学会誌 92 巻 ( 9 ) 頁： 495 - 499 2014年

Orientation based segmentation for phase-contrast microscopic image of confluent cell 査読有り

Sasaki Kei, Yuasa Tetsuya, Sasaki Hiroto, Kato Ryuji

PROCEEDINGS OF THE ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY 頁： 3323 - 3326 2013年10月

Screening of peptide ligands that bind to the Fc region of IgG using peptide array and its application to affinity purification of antibody 査読有り

Sugita Tomoya, Katayama Makoto, Okochi Mina, Kato Ryuji, Ichihara Takamitsu, Honda Hiroyuki

BIOCHEMICAL ENGINEERING JOURNAL 79 巻頁： 33 - 40 2013年10月

Analysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library. 査読有り

Ito Keisuke, Hikida Aya, Kawai Shun, Vu Thi Tuyet Lan, Motoyama Takayasu, Kitagawa Sayuri, Yoshikawa Yuko, Kato Ryuji, Kawarasaki Yasuaki

NATURE COMMUNICATIONS 4 巻頁： 2502 2013年9月

Morphology-based prediction of osteogenic differentiation potential of humanmesenchymal stem cells. 査読有り

Matsuoka Fumiko, Takeuchi Ichiro, Agata Hideki, Kagami Hideaki, Shiono Hirofumi, Kiyota Yasujiro, Honda Hiroyuki, Kato Ryuji

PLOS ONE 8 巻 ( 2 ) 頁： e55082 2013年2月

Systematic analysis of a dipeptide library for inhibitor development using human dipeptidyl peptidase IV produced by a Saccharomyces cerevisiae expression system 査読有り

Hikida Aya, Ito Keisuke, Motoyama Takayasu, Kato Ryuji, Kawarasaki Yasuaki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 430 巻 ( 4 ) 頁： 1217 - 1222 2013年1月

Comprehensive Combination Analysis for Screening of Significant Peptide Epitopes Using a Slide Glass Type-Exclusive Peptide Array from Milk Protein 査読有り

Nakatochi Masahiro, Katayama Makoto, Kato Ryuji, Okochi Mina, Takase Tomokazu, Yoshida Yasuko, Kawase Mitsuo, Honda Hiroyuki

KAGAKU KOGAKU RONBUNSHU 39 巻 ( 1 ) 頁： 40 - 45 2013年

出版者・発行元：The Society of Chemical Engineers, Japan

Based on the hypothesis that development of cow's milk allergy (CMA) in children can diagnosed from species of IgE epitope in patients, significant peptide epitope for IgE was analyzed by comprehensive combination analysis using the graphic assessment method. The Adaboost algorithm was used for classification of decision stump as weak classifier, and 6044060 models were constructed. Classification accuracies by 10-fol CV (10-fold cross-validation) were determined. Total distribution of accuracies was depicted and it was analyzed by Gaussian mixture distribution using the EM algorithm. It was found that the distribution could be divided into 5 sub-distributions by the Bayesian information criterion. The Gaussian sub-distributions were grouped into “significant sub-distributions” and “non-significant sub-distributions” and classifiers that belong to “significant distribution” were identified. Analysis of the assignment frequency of individual inputs assigned to the “significant distribution” identified 16 peptide epitopes with high assignment frequency, including aS1C30 and aS1C47 peptides. Since SERYLGYL from aS1C30 has already been reported as significant peptide epitope for CMA diagnose, our proposed method, CC-GAM, was suggested to be a useful tool for selection of significant epitopes.

DOI： 10.1252/kakoronbunshu.39.40

Web of Science

Scopus

再生促進型医療材料開発のための細胞選択的接着ペプチドの探索と応用

蟹江慧, 加藤竜司, 桑原史明, 成田裕司, 本多裕之

生体医工学 51 巻 ( 3 ) 頁： 202 - 206 2013年

Orientation based segmentation for phase-contrast microscopic image of confluent cell 査読有り

Sasaki Kei, Yuasa Tetsuya, Sasaki Hiroto, Kato Ryuji

2013 35TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) 頁： 3323 - 3326 2013年

実用化に資する医薬品生産培養技術の課題と展開～抗体医薬品から細胞医薬品まで～)

長森英二, 加藤竜司, 清水一憲, 柳原佳奈

生物工学会誌 91 巻 ( 9 ) 2013年

Combinational risk factors ofmetabolic syndrome identified by fuzzy neural network analysis of health-checkdata. 査読有り

Ushida Yasunori, Kato Ryuji, Niwa Kosuke, Tanimura Daisuke, Izawa Hideo, Yasui Kenji, Takase Tomokazu, Yoshida Yasuko, Kawase Mitsuo, Yoshida Tsutomu, Murohara Toyoaki, Honda Hiroyuki

BMC MEDICAL INFORMATICS AND DECISION MAKING 12 巻 ( 1 ) 頁： 80 2012年8月

Screening for silver nanoparticle-binding peptides by using a peptide array 査読有り

Kuboyama Masashi, Kato Ryuji, Okochi Mina, Honda Hiroyuki

BIOCHEMICAL ENGINEERING JOURNAL 66 巻頁： 73 - 77 2012年7月

Collagen type IV-specific tripeptides forselective adhesion of endothelial and smooth muscle cells. 査読有り

Kanie Kei, Narita Yuji, Zhao Yingzi, Kuwabara Fumiaki, Satake Makoto, Honda Susumu, Kaneko Hiroaki, Yoshioka Tomohiko, Okochi Mina, Honda Hiroyuki, Kato Ryuji

BIOTECHNOLOGY AND BIOENGINEERING 109 巻 ( 7 ) 頁： 1808 - 1816 2012年7月

Screening of an α-amylaseinhibitor peptide by photolinker-peptide array. 査読有り

Ochiai Takashi, Sugita Tomoya, Kato Ryuji, Okochi Mina, Honda Hiroyuki

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 76 巻 ( 4 ) 頁： 819 - 824 2012年4月

ヒト Collagen における細胞選択的ペプチドの分布査読有り

蟹江慧, 成田裕司, 桑原史明, 佐竹真, 本多勧, 兼子博章, 本多裕之, 加藤竜司

高分子論文集 69 巻 ( 3 ) 頁： 129 - 134 2012年3月

担当区分：最終著者,　責任著者出版者・発行元：公益社団法人高分子学会

To reduce risks in vascular implants, the design of effective coating polymer for balancing the organization and growth of surrounding cells is important. To design such polymer by learning the mechanism of native extracellular matrix (ECM), we had investigated ECM-specific peptides with cell-selectivity to control the adhesion balance of endothelial cells (ECs) and smooth muscle cells (SMCs). From our high-throughput comparative screening using in silico analysis and peptide array screening platform, we had found that there are various types of EC-selective and SMC-selective peptides in human collagen type IV. In this report, we mapped the distribution of obtained EC-and SMC-selective peptides in the collagen, and extracted a new design policy of cell-selective polymers for medical implant surface modification.

DOI： 10.1295/koron.69.129

Scopus

位相差顕微鏡による細胞画像の領域分割と特徴量抽出 (医用画像)

佐々木啓, 佐々木寛人, 加藤竜司, 湯浅哲也, 武澤康範, 松本阿佐子

電子情報通信学会技術研究報告 : 信学技報 111 巻 ( 389 ) 頁： 343 - 348 2012年1月

出版者・発行元：一般社団法人電子情報通信学会

我々は,位相差顕微鏡によって取得した細胞画像から,細胞領域を自動的に分割し,細胞形態の特徴量を高速で抽出するソフトウェアシステムの構築を目指している.位相差顕微鏡は細胞のリアルタイムモニタリングに適しているが,位相差画像には,細胞と背景の境界に生ずるハロ,および背景の濃度ムラが存在することなどにより,細胞の領域分割を困難にする.本研究では,モルフォロジカルフィルタを使用した細胞領域分割方法を提案する.次に,領域分割によって得られた2値画像から得られるチェインコードを用いて,細胞の特徴量を高速に計算する方法を提案する.最後に,領域分割結果と熟練者がトレースした輪郭とを比較し,提案手法の領域分割性能をF値により評価する.この結果は平均して6割程度であった.

Image-based cell quality assessment: Modeling of cell morphology and quality for clinical cell therapy 査読有り

Sasaki Hiroto, Matsuoka Fumiko, Yamamoto Wakana, Kojima Kenji, Honda Hiroyuki, Kato Ryuji

COMPUTATIONAL MODELING IN TISSUE ENGINEERING 10 巻頁： 207 - 226 2012年1月

Novel small-caliber vascular grafts with trimeric peptide for acceleration of endothelialization. 査読有り

Kuwabara Fumiaki, Narita Yuji, Yamawaki-Ogata Aika, Kanie Kei, Kato Ryuji, Satake Makoto, Kaneko Hiroaki, Oshima Hideki, Usui Akihiko, Ueda Yuichi

ANNALS OF THORACIC SURGERY 93 巻 ( 1 ) 頁： 156 - 163 2012年1月

Enhancement of the Activity of a Lactobacilli-Aggregating Peptide by Freezing Treatment 査読有り

Asai Yuji, Sugita Tomoya, Kato Ryuji, Okochi Mina, Nakagawa Kyuya, Honda Hiroyuki

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 45 巻 ( 8 ) 頁： 609 - 614 2012年

Screening of peptides with a high affinity to bile acids using peptide arraysand a computational analysis. 査読有り

Takeshita Toshiyuki, Okochi Mina, Kato Ryuji, Kaga Chiaki, Tomita Yasuyuki, Nagaoka Satoshi, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 112 巻 ( 1 ) 頁： 92 - 97 2011年4月

The ratio of adiponectinto homeostasis model assessment of insulin resistance is a powerful index ofeach component of metabolic syndrome in an aged Japanese population resultsfrom the KING Study. 査読有り

Nakatochi Masahiro, Miyata Seiko, Tanimura Daisuke, Izawa Hideo, Asano Hiroyuki, Murase Yosuke, Kato Ryuji, Ichihara Sahoko, Naruse Keiko, Matsubara Tatsuaki, Honda Hiroyuki, Yokota Mitsuhiro

DIABETES RESEARCH AND CLINICAL PRACTICE 92 巻 ( 3 ) 頁： 61 - 65 2011年3月

Amino acid sequence preferences to control cell-specific organization of endothelial cells, smooth muscle cells, and fibroblasts. 査読有り国際誌

Kanie Kei, Kato Ryuji, Zhao Yingzi, Narita Yuji, Okochi Mina, Honda Hiroyuki

JOURNAL OF PEPTIDE RESEARCH 17 巻 ( 6 ) 頁： 479 - 486 2011年2月

Gingival and dermal fibroblasts their similarities anddifferences revealed from gene expression. 査読有り

Ebisawa Katsumi, Kato Ryuji, Okada Mai, Sugimura Tomotaka, Abdul Latif Mazlyzam, Hori Yusuke, Narita Yuji, Ueda Minoru, Honda Hiroyuki, Kagami Hideaki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 111 巻 ( 3 ) 頁： 255 - 258 2011年2月

Peptide array-based peptide-cell interaction analysis. 査読有り国際誌

Kato Ryuji, Kaga Chiaki, Kanie Kei, Kunimatsu Mitoshi, Okochi Mina, Honda Hiroyuki

MINI-REVIEWS OF ORGANIC CHEMISTRY 8 巻 ( 2 ) 頁： 171 - 177 2011年

Screening ofpeptides with a high affinity for ZnO using spot-synthesized peptide arrays andcomputational analysis. 査読有り国際誌

Okochi Mina, Ogawa Masatoshi, Kaga Chiaki, Sugita Tomoya, Tomita Yasuaki, Kato Ryuji< Honda Hiroyuki

ACTA BIOMATERIALIA 6 巻 ( 6 ) 頁： 2301 - 2306 2010年12月

Acompact, automated cell culture system for clinical scale cell expansion fromprimary tissues. 査読有り国際誌

Kato Ryuji, Iejima Daisuke, Agata Hideki, Asahina Izumi, Okada Kunihiko, Ueda Minoru, Honda Hiroyuki, Kagami Hideaki

TISSUE ENGINEERING PART C METHODS 16 巻 ( 5 ) 頁： 947 - 956 2010年11月

Novel strategy for prevention ofesophageal stricture after endoscopic surgery. 査読有り国際誌

Mizutani Taro, Tadauchi Akimitsu, Arinobe Manabu, Narita Yuji, Kato Ryuji, Niwa Yasumasa, Ohmiya Naoki, Itoh Akihiro, Hirooka Yoshiki, Honda Hiroyuki, Ueda Minoru, Goto Hidemi

HEPATOGASTROENTEROLOGY 57 巻 ( 102-103 ) 頁： 1150 - 1156 2010年9月

Practical cell labeling with magnetitecationic liposomes for cell manipulation. 査読有り国際誌

Ito Hiroshi, Nonogaki Yurika, Kato Ryuji, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 110 巻 ( 1 ) 頁： 124 - 129 2010年2月

Directed evolution of angiotensin II-inhibiting peptides using a microbeaddisplay. 査読有り国際誌

Gan Rui, Furuzawa Seiji, Kojima Takaaki, Kanie Keie, Kato Ryuji, Okochi Mina, Honda Hiroyuki, Nakano Hideo

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 109 巻 ( 4 ) 頁： 411 - 417 2009年11月

Magnetic manipulation device for theoptimization of cell processing conditions. 査読有り国際誌

Ito Hiroshi, Kato Ryuji, Ino Kosuke, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 109 巻 ( 2 ) 頁： 182 - 188 2009年8月

Peptide array-based analysis of the specific IgE andIgG4 in cow's milk allergens and its use in allergy evaluation. 査読有り国際誌

Matsumoto Naoki, Okochi Mina, Matsushima Miyoko, Kato Ryuji, Takase Tomokazu, Yoshida Yasuko, Kawase Mitsuo, Isobe Ken-ichi, Kawabe Tsutomu, Honda Hiroyuki

PEPTIDES 30 巻 ( 10 ) 頁： 1840 - 1847 2009年7月

Cancer DNA microarrayanalysis considering multi-subclass with graph-based clustering method. 査読有り国際誌

Kawamura Takashi, Hutoh Hironori, Tomita Yasuyuki, Kato Ryuji, Honda Hiroyuk

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 106 巻 ( 5 ) 頁： 442 - 448 2008年12月

Size dependent heat generation of magnetite nanoparticles under AC magneticfield for cancer therapy. 査読有り国際誌

Motoyama Jun, Hakata Toshiyuki, Kato Ryuji, Yamashita Noriyuki, Morino Tomio, Kobayashi Takeshi, Honda Hiroyuki

BIOMAGNETIC RESEARCH AND TECHNOLOGY 6 巻頁： 4 2008年10月

A motif detection and classificationmethod for peptide sequences using genetic programming. 査読有り国際誌

Tomita Yasuyuki, Kato Ryuji, Okochi Mina, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 106 巻 ( 2 ) 頁： 154 - 161 2008年9月

Screening of anovel octamer peptide, CNSCWSKD, that induces caspase-dependent cell death. 査読有り国際誌

Kaga Chiaki, Okochi Mina, Nakanishi Mari, Hayashi Hiroki, Kato Ryuji, Honda Hiroyuki

BIOCHEMICAL AND BIOPHYSICAL RESARCH COMMUNICATIONS 362 巻 ( 4 ) 頁： 1063 - 1068 2008年8月

Computationally assistedscreening and design of cell-interactive peptides by a cell-based assay usingpeptide arrays and a fuzzy neural network algorithm. 査読有り国際誌

Kaga Chiaki, Okochi Mina, Tomoita Yasuyuki, Kato Ryuji, Honda Hiroyuki

BIOTECHNIQUES 44 巻 ( 3 ) 頁： 393 - 402 2008年5月

Identification of HLA-A24-restricted epitopes with high affinities to Hsp70using peptide arrays. 査読有り国際誌

Okochi Mina, Hayashi Hiroki, Ito Akira, Kato Ryuji, Tamura Yasuaki, Sato Noriyuki, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 105 巻 ( 3 ) 頁： 198 - 203 2008年4月

Screening of cell adhesive peptide from the human laminin-5 alpha 3 chain globular 2 and 3 domains. 査読有り国際誌

Okochi Mina, Nomura Shigeyuki, Kaga Chiaki, Honda Hiroyuki

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 41 巻 ( 3 ) 頁： 206 - 209 2008年3月

Cryopreservation of mouse adipose tissue-derived stem/progenitorcells. 査読有り国際誌

Oishi Koichi, Noguchi Hirofumi, Yukawa Hiroshi, Miyazaki Takamichi, Kato Ryuji, Kitagawa Yasuo, Ueda Minoru, Hayashi Shuji

CELL TRANSPLANTATION 17 巻 ( 1-2 ) 頁： 35 - 41 2008年1月

Peptide array-basedinteraction assay of solid-bound peptides and anchorage-dependant cells and itseffectiveness in cell-adhesive peptide design. 査読有り国際誌

Kato Ryuji, Kaga Chiaki, Kunimatsu Mitoshi, Kobayashi Takeshi, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 101 巻 ( 6 ) 頁： 485 - 495 2006年8月

High-throughputscreening of cell death inducible short peptides from TNF-related apoptosis-inducing ligand sequence. 査読有り国際誌

Okochi Mina, Nakanishi Mari, Kato Ryuji, Kobayashi Takeshi, Honda Hiroyuki

FEBS LETTERS 580 巻 ( 3 ) 頁： 885 - 889 2006年1月

Novel strategy for protein exploration high-throughput screening assisted withfuzzy neural network. 査読有り国際誌

Kato Ryuji, Nakano Hideo, Konishi Hiroyuki, Kato Katsuya, Koga Yuchi, Yamane Tsuneo, Kobayashi Takeshi, Honda Hiroyuki

JOURNAL OF MOLECULAR BIOLOGY 351 巻 ( 3 ) 頁： 683 - 692 2005年6月

Angiotensin IIinhibitory peptide found in the receptor sequence using peptide array. 査読有り国際誌

Kato Ryuji, Kunimatsu Mitoshi, Fujimoto Seigo, Kobayashi Takeshi, Honda Hiroyuki

BIOCHEMICAL AND BIOPHYSICAL RESARCH COMMUNICATIONS 315 巻 ( 1 ) 頁： 22 - 29 2004年1月

Hidden Markov model-based approach as the first screening of binding peptides that interact with MHC class II molecules. 査読有り国際誌

Kato Ryuji, Noguchi Hideki, Honda Hiroyuki, Kobayashi Takeshi

ENZYME AND MICROBIAL TECHNOLOGY 33 巻 ( 4 ) 頁： 472 - 481 2003年6月

Hidden Markov model-based prediction of antigenic peptides that interact with MHC class II molecules. 査読有り国際共著国際誌

Noguchi Hideki, Kato Ryuji, Hanai Taizo, Matubara Yukari, Honda Hiroyuki, Vladimir Brusic, Kobayashi Takeshi

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 94 巻 ( 3 ) 頁： 264 - 270 2002年11月

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