Faculty Profiles - KATO, Ryuji

Papers - KATO, Ryuji

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Importance of dataset design in developing robust U-Net models for label-free cell morphology evaluation.

Shiina T, Kimura K, Takemoto Y, Tanaka K, Kato R

Journal of bioscience and bioengineering Vol. 139 ( 4 ) page： 329 - 339 2025.4

Language：English Publisher：Journal of Bioscience and Bioengineering

Advances in regenerative medicine highlighted the need for label-free cell image analysis to replace conventional microscopic observation for non-invasive cell quality evaluation. Image-based evaluation provides an efficient, quantitative, and automated approach to cell analysis, but segmentation remains a critical and challenging step. In this study, we investigated how training dataset design influenced the robustness of U-Net models for cell segmentation, focusing on challenges posed by limited data availability in cell culture. Using 2592 image pairs from four cell types representing key morphological categories, we constructed 42 investigation patterns to evaluate the effects of dataset size, dataset content, and morphological diversity on model performance. Our results showed that robust segmentation models could be developed with approximately 10 raw images captured using a 4× objective lens, a much smaller dataset than typically assumed. The dataset content was found to be crucial: training dataset images that captured commonly observed cell patterns yielded more robust models compared to those capturing rare or irregular cell patterns, which often impaired model performance with large deviations. Additionally, including both spindle and round cell morphologies in the training datasets improved model robustness when tested across all four cell types, while datasets restricted to a single morphology type could not achieve robust models. These findings highlight the importance of curating datasets that capture representative yet diverse cell morphologies. By addressing critical questions about dataset design, this study provides actionable guidance for the effective use of deep learning-based cell segmentation models in manufacturing and research applications.

DOI： 10.1016/j.jbiosc.2025.01.004

Scopus

PubMed

Medicinal cannabis extracts are neuroprotective against Aβ1-42-mediated toxicity in vitro Open Access

Marsh, DT; Shibuta, M; Kato, R; Smid, SD

BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY Vol. 135 ( 5 ) page： 575 - 592 2024.11

Language：English Publisher：Basic and Clinical Pharmacology and Toxicology

Background: Phytocannabinoids inhibit the aggregation and neurotoxicity of the neurotoxic Alzheimer's disease protein β amyloid (Aβ). We characterised the capacity of five proprietary medical cannabis extracts, heated and non-heated, with varying ratios of cannabidiol and Δ9-tetrahydrocannabinol and their parent carboxylated compounds to protect against lipid peroxidation and Aβ-evoked neurotoxicity in PC12 cells. Methods: Neuroprotection against lipid peroxidation and Aβ1–42-induced cytotoxicity was assessed using the thiazolyl blue tetrazolium bromide (MTT) assay. Transmission electron microscopy was used to visualise phytocannabinoid effects on Aβ1–42 aggregation and fluorescence microscopy. Results: Tetrahydrocannabinol (THC)/tetrahydrocannabinolic acid (THCA)-predominant cannabis extracts demonstrated the most significant overall neuroprotection against Aβ1–42-induced loss of PC12 cell viability. These protective effects were still significant after heating of extracts, while none of the extracts provided significant neuroprotection to lipid peroxidation via tbhp exposure. Modest inhibition of Aβ1–42 aggregation was demonstrated only with the non-heated BC-401 cannabis extract, but overall, there was no clear correlation between effects on fibrils and conferral of neuroprotection. Conclusions: These findings highlight the variable neuroprotective activity of cannabis extracts containing major phytocannabinoids THC/THCA and cannabidiol (CBD)/cannabidiolic acid (CBDA) on Aβ-evoked neurotoxicity and inhibition of amyloid β aggregation. This may inform the future use of medicinal cannabis formulations in the treatment of Alzheimer's disease and dementia.

DOI： 10.1111/bcpt.14078

Editorial: Artificial intelligence in predicting, determining and controlling cell phenotype or tissue function in inflammatory diseases Open Access

Hart, ML; Kato, R; Rolauffs, B

FRONTIERS IN IMMUNOLOGY Vol. 15 page： 1443534 2024.6

Construction of transcript regulation mechanism prediction models based on binding motif environment of transcription factor AoXlnR in Aspergillus oryzae

Oka, H; Kojima, T; Kato, R; Ihara, K; Nakano, H

JOURNAL OF BIOINFORMATICS AND COMPUTATIONAL BIOLOGY Vol. 22 ( 03 ) page： 2450017 2024.6

Language：English Publisher：Journal of Bioinformatics and Computational Biology

DNA-binding transcription factors (TFs) play a central role in transcriptional regulation mechanisms, mainly through their specific binding to target sites on the genome and regulation of the expression of downstream genes. Therefore, a comprehensive analysis of the function of these TFs will lead to the understanding of various biological mechanisms. However, the functions of TFs in vivo are diverse and complicated, and the identified binding sites on the genome are not necessarily involved in the regulation of downstream gene expression. In this study, we investigated whether DNA structural information around the binding site of TFs can be used to predict the involvement of the binding site in the regulation of the expression of genes located downstream of the binding site. Specifically, we calculated the structural parameters based on the DNA shape around the DNA binding motif located upstream of the gene whose expression is directly regulated by one TF AoXlnR from Aspergillus oryzae, and showed that the presence or absence of expression regulation can be predicted from the sequence information with high accuracy (AUPRC=0.8-1.0) by machine learning incorporating these parameters.

DOI： 10.1142/S0219720024500173

Web of Science

Scopus

PubMed

Image-based hybrid model incorporating initial spatial distribution for mesenchymal stem cell cultivation process design

Hirono, K; Hayashi, Y; Udugama, IA; Takemoto, Y; Kato, R; Kino-oka, M; Sugiyama, H

AICHE JOURNAL 2024.4

Publisher：AIChE Journal

This work presents an image-based hybrid model incorporating the initial spatial distribution for mesenchymal stem cell (MSC) cultivation process design. First, three levels of seeding bias in static MSC cultivation were experimentally investigated, and the resulting initial distribution was quantified using phase contrast microscopy image analysis. Second, the observed spatial heterogeneity was defined as a new parameter by calculating the standard deviation of the seeded cell number fraction among numerical 8 × 8 tiles on two-dimensional plates. Third, the parameter was incorporated into kinetic models to consider spatial growth limitation. The model was then applied to simulate MSC cultivation processes with experimental data. Using dynamic and stochastic simulation outputs, feasible ranges of cell-harvesting time could be determined to satisfy a given requirement for the minimum cell number and acceptable confluency level. The developed hybrid model could serve as a basis for quantitative decision-making in the design of MSC cultivation processes.

DOI： 10.1002/aic.18452

Web of Science

Scopus

Prediction of antibody production performance change in Chinese hamster ovary cells using morphological profiling. Reviewed

Hisada T, Imai Y, Takemoto Y, Kanie K, Kato R

Journal of bioscience and bioengineering 2024.3

Development of novel waxy bone haemostatic agents composed of biodegradable polymers with osteogenic-enhancing peptides in rabbit models Reviewed

Ohno, T; Suenaga, H; Yamawaki-Ogata, A; Kanie, K; Kato, R; Uto, K; Ebara, M; Ito, H; Narita, Y; Usui, A; Mutsuga, M

Interdisciplinary cardiovascular and thoracic surgery Vol. 37 ( 5 ) 2023.11

The structurally diverse phytocannabinoids cannabichromene, cannabigerol and cannabinol significantly inhibit amyloid β-evoked neurotoxicity and changes in cell morphology in PC12 cells. Reviewed International coauthorship

Marsh DT, Sugiyama A, Imai Y, Kato R, Smid SD

Basic & clinical pharmacology & toxicology 2023.9

Automated cell isolation from photodegradable hydrogel based on fluorescence image analysis. Reviewed

Shinji Sugiura, Shinya Yamahira, Masato Tamura, Kazumi Shin, Mayu Shibuta, Taku Satoh, Yui Matsuzawa, Gen Fujii, Fumiki Yanagawa, Michihiro Mutoh, Masumi Yanagisawa, Ryuji Kato, Hirofumi Matsui

BIOTECHNOLOGY AND BIOENGINEERING 2023.3

Novel functional peptide for next-generation vital pulp therapy Reviewed

Watanabe Masakatsu, Okamoto Motoki, Komichi Shun, Huang Hailing, Matsumoto Sayoko, Moriyama Kiichi, Ohshima Jun, Abe Shotaro, Morita Masayoshi, Ali Manahil,, Takebe Katsuki, Kozaki Ikko, Fujimoto Akiyo, Kanie Kei, Kato Ryuji, Uto Kenichiro, Ebara Mitsuhiro, Yamawaki-Ogata Aika, Narita Yuji, Takahashi Yusuke, Hayashi Mikako

JOURNAL OF DENTAL RESEARCH Vol. 102 ( 3 ) page： 322 - 330 2023.3

Bioinformatics in bioscience and bioengineering: Recent advances, applications, and perspectives Reviewed

Uesaka Kazuma, Oka Hiroya, Kato Ryuji, Kanie Kei, Kojima Takaaki, Tsugawa Hiroshi, Toda Yosuke, Horinouchi Takaaki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 134 ( 5 ) page： 363 - 373 2022.11

天野日出, 木村和恵, 今井佑太, 加藤竜司, 中澤温子, 城田千代栄, 滝本愛太朗, 住田亙, 牧田智, 岡本眞宗, 安井昭洋, 高田瞬也, 中川洋一, 加藤大幾, 檜顕成, 内田広夫

小児外科 Vol. 54 ( 10 ) page： 1007 - 1011 2022.10

Morphology-based noninvasive early prediction of serial-passage potency enhances the selection of clone-derived high-potency cell bank from mesenchymal stem cells Reviewed

Suyama Takashi, Takemoto Yuto, Miyauchi Hiromi, Kato Yuko, Matsuzaki Yumi, Kato Ryuji

INFLAMMATION AND REGENERATION Vol. 42 ( 1 ) page： 30 2022.10

Label-free morphological sub-population cytometry for sensitive phenotypic screening of heterogenous neural disease model cells Reviewed

Imai Yuta, Iida Madoka, Kanie Kei, Katsuno Masahisa, Kato Ryuji

SCIENTIFIC REPORTS Vol. 12 ( 1 ) page： 9296 2022.6

Bi-layered carboxymethyl cellulose-collagen vitrigel dual-surface adhesion-prevention membrane Reviewed

Wang Yue, Kanie Kei, Takezawa Toshiaki, Horikawa Miki, Kaneko Kyoshiro, Sugimoto Ayako, Yamawaki-Ogata Aika, Narita Yuji, Kato Ryuji

CARBOHYDRATE POLYMERS Vol. 285 page： 119223 2022.6

Myc supports self-renewal of basal cells in the esophageal epithelium. Reviewed International coauthorship

Tomoaki Hishida, Eric Vazquez-Ferrer, Yuriko Hishida-Nozaki, Yuto Takemoto, Fumiyuki Hatanaka, Kei Yoshida, Javier Prieto, Sanjeeb Kumar Sahu, Yuta Takahashi, Pradeep Reddy, David D O'Keefe, Concepcion Rodriguez Esteban, Paul S Knoepfler, Estrella Nuñez Delicado, Antoni Castells, Josep M Campistol, Ryuji Kato, Hiroshi Nakagawa, Juan Carlos Izpisua Belmonte

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY Vol. 10 page： 786031 2022.3

Collection of Data Variation Using a High-Throughput Image-Based Assay Platform Facilitates Data-Driven Understanding of TRPA1 Agonist Diversity Reviewed

Terada Yuko, Tanaka Kenjiro, Matsuyama Minami, Fujitani Masaya, Shibuya Masatoshi, Yamamoto Yoshihiko, Kato Ryuji, Ito Keisuke

APPLIED SCIENCES Vol. 12 ( 3 ) 2022.2

Morphological heterogeneity description enabled early and parallel non-invasive prediction of T-cell proliferation inhibitory potency and growth rate for facilitating donor selection of human mesenchymal stem cells Reviewed

Imai Yuta, Kanie Kei, Kato Ryuji

INFLAMMATION AND REGENERATION Vol. 42 ( 1 ) page： 8 2022.1

Enhancement of protein thermostability by three consecutive mutations using loop-walking method and machine learning Reviewed

Yoshida Kazunori, Kawai Shun, Fujitani Masaya, Koikeda Satoshi, Kato Ryuji, Ema Tadashi

SCIENTIFIC REPORTS Vol. 11 ( 1 ) page： 11883 2021.6

Predicting quality decay in continuously passaged mesenchymal stem cells by detecting morphological anomalies.  Reviewed

Takemoto Yuto, Imai Yuta, Kanie Kei, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 131 ( 2 ) page： 198 - 206 2021.2

Development of Carbon Concentration Prediction Technology from Microstructure Images of Carburizing Heat Treatment Products using Machine Learning Reviewed

YACHI Yoshiki, HATTORI Kiyoyuki, KATO Ryuji

NETSUSHORI Vol. 61 ( 5 ) page： 257 - 263 2021

Language：Japanese Publisher：The Japan Society for Heat Treatment

Carburizing, quenching and tempering heat treatments are widely used as surface hardening, and it is known that their performances are changed depending on the carbon concentration. We had verified the effectiveness of the technique for predicting the carbon concentration from the microstructure images after heat treatment by using image recognition technologies and machine learning technologies that were focused in recent years. These technologies extracted the black and white objects of the image, and then machine learning of each shape information and brightness information as a features．Based on these technologies, we succeeded in predicting 0.78 mass％ of microstructure in the range of 0.75 mass％ to 0.79 mass％ by using regression models according to the characters of microstructure images.

DOI： 10.14940/netsushori.61.257

CiNii Research

身近となった画像解析とAI

加藤竜司

生物工学会誌 Vol. 99 ( 8 ) page： 432 - 435 2021

Screening of FFAR1-activating peptides by molecular structural analysis Reviewed

Yoshioka Keitaro, Yamashita Haruki, Fujitani Masaya, Kato Ryuji, Shimizu Kazunori, Honda Hiroyuki

KAGAKU KOGAKU RONBUNSHU Vol. 47 ( 3 ) page： 64 - 68 2021

Language：Japanese Publisher：The Society of Chemical Engineers, Japan

To find FFAR1-activating peptides consisting of natural amino acid residues, molecular structure analysis was performed by hierarchical clustering using extraction of physicochemical parameters. When 3 mer to 6 mer peptide libraries were prepared and the molecular structure of each was compared with 5 chemical agonists, 12 peptides with high correlation coefficients of physicochemical parameters were found from the 6 mer peptide library. These peptides were then synthesized by Fmoc solid phase synthesis, and their activity was assessed by TGFα shedding assay using FFAR1-expressing HEK293. As a result, two peptides, GCGGSS and GASGCC, were identified as peptide agonists with relatively high activity. Although these peptides showed approximately one fifth of the activity of chemical agonist GW9508, they are the first examples that we know of FFAR1-activating peptides consisting of natural amino acid residues.

DOI： 10.1252/kakoronbunshu.47.64

Web of Science

Scopus

Machine learning-based amino-acid substitution of short peptides: Acquisition of peptides with enhanced inhibitory activities against α-amylase and α-glucosidase. Reviewed

Yamashita Haruki, Fujitani Masaya, Shimizu Kazunori, Kanie Kei, Kato Ryuji, Honda Hiroyuki

ACS BIOMATERIALS SCIENCE & ENGINEERING Vol. 6 ( 11 ) page： 6117 - 6125 2020.11

In vitro model of human skeletal muscle tissues with contractility fabricated by immortalized human myogenic cells. Reviewed

Nagashima Takunori, Hadiwidjaja Stacy, Ohsumi Saki, Murata Akari, Hisada Takumi, Kato Ryuji, Okada Yohei, Honda Hiroyuki, Shimizu Kazunori

ADVANCED BIOSYSTEMS Vol. 4 ( 11 ) page： e2000121 2020.11

Reproducible production and image-based quality evaluation of retinalpigment epithelium sheets from human induced pluripotent stem cells. Reviewed

Ye Ke, Takemoto Yuto, Ito Arisa, Onda Masanari, Morimoto Nao, Mandai Michiko, Takahashi Masayo, Kato Ryuji, Osakada Fumitaka

SCIENTIFIC REPORTS Vol. 10 ( 1 ) page： 14387 2020.9

The importance of scoring recognition fitness in spheroid morphological analysis for robust label -free quality evaluation Reviewed

Shirai Kazuhide, Kato Hirohito, Imai Yuta, Shibuta Mayu, Kanie Kei, Kato Ryuji

REGENERATIVE THERAPY Vol. 14 page： 205 - 214 2020.6

Performance of a biodegradable compositewith hydroxyapatite as a scaffold in pulp tissue repair. Reviewed

Okamoto Motoki, Matsumoto Sayako, Sugiyama Ayato, Kanie Kei, Watanabe Masakatsu, Huang Hailing, Ali Manahil, Ito Yuki, Miura Jiro, Hirose Yujiro, Uto Koichiro, Ebara Mitsuhiro, Kato Ryuji, Yamawaki-Ogata Aika, Narita Yuji, Kawabata Shigetada, Takahashi Yusuke, Hayashi Mikako

POLYMERS (BASEL) Vol. 12 ( 4 ) page： 937 - 941 2020.4

Novel cannabis flavonoid, cannflavin A displays both a hormetic and neuroprotective profile against amyloid β-mediatedneurotoxicity in PC12 cells: Comparison with geranylated flavonoids, mimuloneand diplacone. Reviewed International coauthorship

Eggers Carly, Fujitani Masaya, Kato Ryuji, Smid Scott

BIOCHEMICAL PHARMACOLOGY Vol. 169 page： 113609 2019.8

Effect of mechanical vibration stress in cell culture on human inducedpluripotent stem cells. Reviewed

Kanie Kei, Sakai Teppei, Imai Yuta, Yoshida Kei, Sugimoto Ayako, Makino Hodaka, Kubo Hirotsugu, Kato Ryuji

REGENERATIVE THERAPY Vol. 12 page： 27 - 35 2019.6

Quantitative analysisof operators' flow line in the cell culture for controlled manual operation. Reviewed

Kanie Kei, Sasaki Hiroto, Ikeda Yurika, Tamada Masaki, Togawa Fumio, Kato Ryuji

REGENERATIVE THERAPY Vol. 12 page： 43 - 54 2019.5

Identification of an early cell fate regulator bydetecting dynamics in transcriptional heterogeneity and co-regulation duringastrocyte differentiation. Reviewed

Ando Tatsuya, Kato Ryuji, Honda Hiroyuki

NPJ SYSTEMS BIOLOGY AND APPLICATIONS Vol. 5 ( 1 ) page： 18 2019.5

Numerical investigation of particle dispersion in the preprocessing stage for a staticcell cultivation. Reviewed

Sekimoto Atsushi, Kanemaru Yoshiki, Okano Yasunori, Kanie Kei, Kato Ryuji, Kino-oka Masahiro

REGENERATIVE THERAPY Vol. 12 ( 83 ) page： 87 2019.4

Activation of cancer-related and mitogen-activated protein kinase signaling pathways in human mature osteoblasts isolated from patients with type 2 diabetes. Reviewed

Kuroiwa Tomoyuki, Matsumoto Megumi, Kato Ryuji, Nimura Akimoto, Yoshii Toshitaka, Okawa Atsushi, Fujita Koji

BONE REPORTS Vol. 10 page： 100199 2019.2

Time-course colony tracking analysis for evaluating induced pluripotent stem cellculture processes. Reviewed

Yoshida Kei, Okada Mai, Nagasaka Risako, Sasaki Hiroto, Kanie Kei, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 128 ( 2 ) page： 209 - 217 2019.2

Morphology-based analysis of myoblasts for prediction of myotube formation. Reviewed

Ishikawa Kiyoshi, Yoshida Kei, Kanie Kei, Omori Kenji, Kato Ryuji

SLAS DISCOVERY Vol. 24 ( 1 ) page： 47 - 56 2019.1

Image-based Morphometric Analysis for Evaluation of CellularResponses

Kato Ryuji

Abstracts for Annual Meeting of Japanese Proteomics Society Vol. 2019 ( 0 ) page： 131 - 131 2019

In-process evaluation of culture errors using morphology-based image analysis. Reviewed

Imai Yuta, Yoshida Kei, Matsumoto Megumi, Okada Mai, Kanie Kei, Shimizu Kazunori, Honda Hiroyuki, Kato Ryuji

REGENERATIVE THERAPY Vol. 9 ( 15 ) page： 15 - 23 2018.12

Effective screening for hypolipidemic bile acid-binding peptides by hierarchical clustering-based catalog array. Reviewed

Kato Ryuji, Kanie Kei, Kaga Chiaki, Okochi Mina, Nagaoka Satoshi, Honda Hiroyuki

JOURNAL OF BIOSCIENCE BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY Vol. 1 ( 1 ) page： doi: 10.31546/BBAB.1001 2018.8

Imaging cell picker: A morphology-based automatedcell separation system on a photodegradable hydrogel culture platform. Reviewed

Mayu Shibuta, Masato Tamura, Kei Kanie, Masumi Yanagisawa, Hirofumi Matsui, Taku Satoh, Toshiyuki Takagi, Toshiyuki Kanamori, Shinji Sugiura, Ryuji Kato

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 126 ( 5 ) page： 653 - 660 2018.6

Development of image-based informatics as non-invasive quality control technology for controlling regenerative medicine product

Kato Ryuji

SEIBUTSU KOUGAKU KAISHI Vol. 96 ( 3 ) page： 121 - 128 2018

Cell Image-Based Informatics for Control of Cell-Based Therapeutic Product Manufacturing

KATO Ryuji

Journal of the Japan Society for Precision Engineering Vol. 84 ( 11 ) page： 901 - 904 2018

Morphology-based non-invasive quantitative prediction of the differentiationstatus of neural stem cells. Reviewed

Fujitani Masaya, Huddin Noor Safika, Kawai Shun, Kanie Kei, Kiyota Yasujiro, Shimizu Kazunori, Honda Hiroyuki, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 124 ( 3 ) page： 351 - 358 2017.9

Heat-stimuli-enhanced osteogenesis using clinically available biomaterials. Reviewed

Ota Takehiro, Nishida Yoshihiro, Ikuta Kunihiro, Kato Ryuji, Kozawa Eiji, Hamada Shunsuke, Sakai Tomohisa, Ishiguro Naoki

PLOS ONE Vol. 12 ( 7 ) page： e0181404 2017.7

Visualization of morphological categories of colonies formonitoring of effect on induced pluripotent stem cell culture status. Reviewed

Nagasaka Risako, Matsumoto Megumi, Okada Mai, Sasaki Hiroto, Kanie Kei, Kii Hiroaki, Uozumi Takayuki, Kiyota Yasujiro, Honda Hiroyuki, Kato Ryuji

REGENERATIVE THERAPY Vol. 6 page： 41 - 51 2017.6

Image-based cell quality evaluation to detect irregularities under same cultureprocess of human induced pluripotent stem cells. Reviewed

Nagasaka Risako, Gotou Yuto, Yoshida Kei, Kanie Kei, Shimizu Kazunori, Honda Hiroyuki, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 123 ( 5 ) page： 642 - 650 2017.5

Prediction of differentiation tendency toward hepatocytes from gene expression in undifferentiated human pluripotent stem cells. Reviewed

Yanagihara Kana, Liu Yujung, Kanie Kei, Takayama Kazuo, Kokunugi Minako, Hirata Mitsuhi, Fukuda Takayuki, Suga Mika, Nikawa Hiroki, Mizuguchi Hiroyuki, Kato Ryuji, Furue-Kusuda Miho

STEM CELLS AND DEVELOPMENT Vol. 25 ( 24 ) page： 1884 - 1897 2016.12

Focused screening of ECM-selective adhesion peptides on cellulose-bound peptide microarrays. Reviewed

Kanie Kei, Kondo Yuto, Owaki Junki, Ikeda Yurika, Narita Yuji, Kato Ryuji, Honda Hiroyuki

BIOENGINEERING (BASEL) Vol. 3 ( 4 ) page： 31 2016.11

Combinational effects of polymer viscoelasticity and immobilized peptides on cell adhesion to cell-selective scaffolds. Reviewed

Kurimoto Rio, Kanie Kei, Uto Koichiro, Kawai Shun, Hara Mitsuo, Nagano Shusaku, Narita Yuji, Honda Hiroyuki, Naito Masanobu, Ebara Mitsuhiro, Kato Ryuji

ANALYTICAL SCIENCES Vol. 32 ( 11 ) page： 1195 - 1202 2016.11

Parametric analysis of colony morphology of non-labelled live human pluripotent stem cells for cell quality control. Reviewed

Kato Ryuji, Matsumoto Megumi, Sasaki Hiroto, Joto Risako, Okada Mai, Ikeda Yurika, Kanie Kei, Suga Mika, Kinehara Masaki, Yanagihara Kana, Liu Yujung, Uchio-Yamada Kozue, Fukuda Takayuki, Kii Hiroaki, Uozumi Takayuki, Honda Hiroyuki, Kiyota Yasujiro, Furue-Kusuda Miho

SCIENTIFIC REPORTS Vol. 6 page： 34009 2016.9

Exploring high-affinity binding properties of octamer peptides by principal component analysisof tetramer peptides. Reviewed

Kume Akiko, Kawai Shun, Kato Ryuji, Iwata Shinmei, Shimizu Kazunori, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 123 ( 2 ) page： 230 - 238 2016.9

Screeningof osteogenic-enhancing short peptides from BMPs for biomimetic material applications. Reviewed

Kanie Kei, Kurimoto Rio, Tian Jing, Ebisawa Katsumi, Narita Yuji, Honda Hiroyuki, Kato Ryuji

MATERIALS (BASEL) Vol. 9 ( 9 ) page： 730 2016.9

Morphological evaluation of nonlabeled cells to detect stimulation of nerve growth factor expression by Lyconadin B. Reviewed

Kawai Shun, Sasaki Hiroto, Okada Norihiro, Kanie Kei, Yokoshima Satoshi, Fukuyama Tohru, Honda Hiroyuki, Kato Ryuji

JOURNAL OF BIOMOLECULAR SCREENING Vol. 21 ( 8 ) page： 795 - 803 2016.9

Non-invasive quality evaluation of confluent cells by image-based orientation heterogeneity analysis Reviewed

Sasaki Kei, Sasaki Hiroto, Takahashi Atsuki, Kang Siu, Yuasa Tetsuya, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 121 ( 2 ) page： 227 - 234 2016.2

Comparisons of cellculture medium using distribution of morphological features in microdevice. Reviewed

Sasaki Hiroto, Enomoto Junko, Ikeda Yurika, Honda Hiroyuki, Fukuda Junji, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 121 ( 1 ) page： 117 - 123 2016.1

Combinational effect of cell adhesion biomolecules and their immobilized polymer property to enhance cell-selective adhesion. Reviewed

Kurimoto Rio, Kei Kanie, Koichiro Uto, Shun Kawai, Mitsuo Hara, Shusaku Nagano, Yuji Narita, Hiroyuki Honda, Masanobu Naito, Mitsuhiro Ebara, Ryuji Kato

INTERNATIONAL JOURNAL OF POLYMER SCIENCE Vol. 2016 page： 2090985 2016

Combinational effects of polymer viscoelasticity and immobilized peptides on cell adhesion to cell-selective scaffolds. Reviewed

Kurimoto Rio, Kei Kanie, Koichiro Uto, Shun Kawai, Mitsuo Hara, Shusaku Nagano, Yuji Narita, Hiroyuki Honda, Masanobu Naito, Mitsuhiro Ebara, Ryuji Kato

ANALYTICAL SCIENCES Vol. 32 ( 11 ) page： 1195 - 1202 2016

Differential variability and correlation of geneexpression identifies key genes involved in neuronal differentiation. Reviewed

Ando Tatsuya, Kato Ryuji, Honda Hiroyuki

BMC SYSTEMS BIOLOGY Vol. 9 ( 1 ) page： 82 2015.11

Image-based focusedcounting of dividing cells for non-invasive monitoring of regenerative medicineproducts. Reviewed

Sasaki K, Miyata H, Sasaki H, Kang S, Yuasa T, Kato R.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 120 ( 5 ) page： 582 - 590 2015.11

Image-based focused counting of dividing cells for non-invasive monitoring of regenerative medicine products Reviewed

Sasaki Kei, Miyata Hirofumi, Sasaki Hiroto, Kang Siu, Yuasa Tetsuya, Kato Ryuji

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 120 ( 5 ) page： 582 - 590 2015.11

Effects of theproperties of short peptides conjugated with cell-penetrating peptides on theirinternalization into cells. Reviewed

Matsumoto Ryo, Okochi Mina, Shimizu Kazunori, Kanie Kei, Kato Ryuji, Honda Hiroyuki

SCIENTIFIC REPORTS Vol. 5 page： 12884 2015.8

Non-invasive quality evaluation of confluent cells by orientation analysis

Sasaki Kei, Sasaki Hiroto, Takahashi Atsuki, Kanie Kei, Kang Siu, Kato Ryuji, Yuasa Tetsuya

IEICE TECHNICAL REPORT Vol. 114 ( 482 ) page： 167 - 172 2015.3

Publisher：The Institute of Electronics, Information and Communication Engineers

Although regenerative medicine is rapidly evolving toward clinical application in recent years, the fact that no effective technique to non-invasively and objectively monitor the state of cultured cells is existent hampers its realization. For this problem, an image processing/recognition technique using phase-contrast microscopy images acquired non-invasively, which first segments individual cells and then quantitatively evaluates the quality of each cell morphologically, will be a solution. However, for confluent cells it has been ineffective because the confluent cells have no decisive boundary, and then each cell cannot be segmented and characterized by the morphological features. In this research, we devise a method to quantify the state of confluent cells by scoring each pixel based on the collective feature in terms of orientation and then statistically characterizing the heterogeneity of the score distribution throughout an image. We demonstrate the efficacy of the proposed method by classifying actual microscopic images of confluent cells into proper classes using a support vector machine based on the feature derived from the orientation and heterogeneity.

Identification of an interactionbetween VWF rs7965413 and platelet count as a novel risk marker for metabolicsyndrome an extensive search of candidate polymorphisms in a case-controlstudy. Reviewed

Nakatochi Masahiro, Ushida Yasunori, Yasuda Yoshinari, Yoshida Yasuko, Kawai Shun, Kato Ryuji, Nakashima Toru, Iwata Masamitsu, Kuwatsuka Yachiyo, Ando Masahiko, Hamajima Nobuyuki, Kondo Takaaki, Oda Hiroaki, Hayashi Mutsuharu, Kato Sawako, Yamaguchi Makoto, Maruyama Shoichi, Matsuo Seiichi, Honda Hiroyuki

PLOS ONE Vol. 10 ( 2 ) page： e0117591 2015.2

In vivo heat-stimulus-triggered osteogenesis. Reviewed

Ikuta Kunihiro, Urakawa Hiroshi, Kozawa Eiji, Hamada Shunsuke, Ota Takehiro, Kato Ryuji, Honda Hiroyuki, Kobayashi Takeshi, Ishiguro Naoki, Nishida Yoshihiro

INTERNATIONAL JOURNAL OF HYPERTHERMIA Vol. 31 ( 1 ) page： 58 - 66 2015.2

Characterization of time-course morphological features for efficientprediction of osteogenic potential in human mesenchymal stem cells. Reviewed

Matsuoka Fumiko, Takeuchi Ichiro, Agata Hideki, Kagami Hideaki, Shiono Hirofumi, Kiyota Yasujiro, Honda Hiroyuki, Kato Ryuji

BIOTECHNOLOGY AND BIOENGINEERING Vol. 111 ( 7 ) page： 1430 - 1439 2014.7

Label-free morphology-based prediction of multiple differentiation potentials ofhuman mesenchymal stem cells for early evaluation of intact cells. Reviewed

Sasaki Hiroto, Takeuchi Ichiro, Okada Mai, Sawada Rumi, Kanie Kei, Kiyota Yasujiro, Honda Hiroyuki, Kato Ryuji

PLOS ONE Vol. 9 ( 4 ) page： e93952 2014.4

The screening strategy of functional peptides derived from scaffold protein by peptide array and its possibility

Kanie Kei, Kato Ryuji

KAGAKU TO SEIBUTSU Vol. 52 ( 6 ) page： 361 - 370 2014

培養中の幹細胞品質評価 : 画像を用いた評価技術とその貢献 (特集再生医療実現に向けた幹細胞培養工学の最前線)

加藤竜司, 清田泰次郎, 備瀬竜馬

生物工学会誌 Vol. 92 ( 9 ) page： 495 - 499 2014

Orientation based segmentation for phase-contrast microscopic image of confluent cell Reviewed

Sasaki Kei, Yuasa Tetsuya, Sasaki Hiroto, Kato Ryuji

PROCEEDINGS OF THE ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY page： 3323 - 3326 2013.10

Screening of peptide ligands that bind to the Fc region of IgG using peptide array and its application to affinity purification of antibody Reviewed

Sugita Tomoya, Katayama Makoto, Okochi Mina, Kato Ryuji, Ichihara Takamitsu, Honda Hiroyuki

BIOCHEMICAL ENGINEERING JOURNAL Vol. 79 page： 33 - 40 2013.10

Analysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library. Reviewed

Ito Keisuke, Hikida Aya, Kawai Shun, Vu Thi Tuyet Lan, Motoyama Takayasu, Kitagawa Sayuri, Yoshikawa Yuko, Kato Ryuji, Kawarasaki Yasuaki

NATURE COMMUNICATIONS Vol. 4 page： 2502 2013.9

Morphology-based prediction of osteogenic differentiation potential of humanmesenchymal stem cells. Reviewed

Matsuoka Fumiko, Takeuchi Ichiro, Agata Hideki, Kagami Hideaki, Shiono Hirofumi, Kiyota Yasujiro, Honda Hiroyuki, Kato Ryuji

PLOS ONE Vol. 8 ( 2 ) page： e55082 2013.2

Systematic analysis of a dipeptide library for inhibitor development using human dipeptidyl peptidase IV produced by a Saccharomyces cerevisiae expression system Reviewed

Hikida Aya, Ito Keisuke, Motoyama Takayasu, Kato Ryuji, Kawarasaki Yasuaki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Vol. 430 ( 4 ) page： 1217 - 1222 2013.1

Comprehensive Combination Analysis for Screening of Significant Peptide Epitopes Using a Slide Glass Type-Exclusive Peptide Array from Milk Protein Reviewed

Nakatochi Masahiro, Katayama Makoto, Kato Ryuji, Okochi Mina, Takase Tomokazu, Yoshida Yasuko, Kawase Mitsuo, Honda Hiroyuki

KAGAKU KOGAKU RONBUNSHU Vol. 39 ( 1 ) page： 40 - 45 2013

Publisher：The Society of Chemical Engineers, Japan

Based on the hypothesis that development of cow's milk allergy (CMA) in children can diagnosed from species of IgE epitope in patients, significant peptide epitope for IgE was analyzed by comprehensive combination analysis using the graphic assessment method. The Adaboost algorithm was used for classification of decision stump as weak classifier, and 6044060 models were constructed. Classification accuracies by 10-fol CV (10-fold cross-validation) were determined. Total distribution of accuracies was depicted and it was analyzed by Gaussian mixture distribution using the EM algorithm. It was found that the distribution could be divided into 5 sub-distributions by the Bayesian information criterion. The Gaussian sub-distributions were grouped into “significant sub-distributions” and “non-significant sub-distributions” and classifiers that belong to “significant distribution” were identified. Analysis of the assignment frequency of individual inputs assigned to the “significant distribution” identified 16 peptide epitopes with high assignment frequency, including aS1C30 and aS1C47 peptides. Since SERYLGYL from aS1C30 has already been reported as significant peptide epitope for CMA diagnose, our proposed method, CC-GAM, was suggested to be a useful tool for selection of significant epitopes.

DOI： 10.1252/kakoronbunshu.39.40

Web of Science

Scopus

Screening and application of cell-selective adhesion peptide for regenerative medical materials

Vol. 51 ( 3 ) page： 202 - 206 2013

Orientation based segmentation for phase-contrast microscopic image of confluent cell Reviewed

Sasaki Kei, Yuasa Tetsuya, Sasaki Hiroto, Kato Ryuji

2013 35TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) page： 3323 - 3326 2013

実用化に資する医薬品生産培養技術の課題と展開～抗体医薬品から細胞医薬品まで～)

長森英二, 加藤竜司, 清水一憲, 柳原佳奈

生物工学会誌 Vol. 91 ( 9 ) 2013

Combinational risk factors ofmetabolic syndrome identified by fuzzy neural network analysis of health-checkdata. Reviewed

Ushida Yasunori, Kato Ryuji, Niwa Kosuke, Tanimura Daisuke, Izawa Hideo, Yasui Kenji, Takase Tomokazu, Yoshida Yasuko, Kawase Mitsuo, Yoshida Tsutomu, Murohara Toyoaki, Honda Hiroyuki

BMC MEDICAL INFORMATICS AND DECISION MAKING Vol. 12 ( 1 ) page： 80 2012.8

Screening for silver nanoparticle-binding peptides by using a peptide array Reviewed

Kuboyama Masashi, Kato Ryuji, Okochi Mina, Honda Hiroyuki

BIOCHEMICAL ENGINEERING JOURNAL Vol. 66 page： 73 - 77 2012.7

Collagen type IV-specific tripeptides forselective adhesion of endothelial and smooth muscle cells. Reviewed

Kanie Kei, Narita Yuji, Zhao Yingzi, Kuwabara Fumiaki, Satake Makoto, Honda Susumu, Kaneko Hiroaki, Yoshioka Tomohiko, Okochi Mina, Honda Hiroyuki, Kato Ryuji

BIOTECHNOLOGY AND BIOENGINEERING Vol. 109 ( 7 ) page： 1808 - 1816 2012.7

Screening of an α-amylaseinhibitor peptide by photolinker-peptide array. Reviewed

Ochiai Takashi, Sugita Tomoya, Kato Ryuji, Okochi Mina, Honda Hiroyuki

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY Vol. 76 ( 4 ) page： 819 - 824 2012.4

Cell-Selective Peptide Distribution in Human Collagen Proteins Reviewed

KOBUNSHI RONBUNSHU Vol. 69 ( 3 ) page： 129 - 134 2012.3

Authorship：Last author,　Corresponding author Publisher：The Society of Polymer Science, Japan

To reduce risks in vascular implants, the design of effective coating polymer for balancing the organization and growth of surrounding cells is important. To design such polymer by learning the mechanism of native extracellular matrix (ECM), we had investigated ECM-specific peptides with cell-selectivity to control the adhesion balance of endothelial cells (ECs) and smooth muscle cells (SMCs). From our high-throughput comparative screening using in silico analysis and peptide array screening platform, we had found that there are various types of EC-selective and SMC-selective peptides in human collagen type IV. In this report, we mapped the distribution of obtained EC-and SMC-selective peptides in the collagen, and extracted a new design policy of cell-selective polymers for medical implant surface modification.

DOI： 10.1295/koron.69.129

Scopus

Segmentation and feature extraction of cell images with phase-contrast microscope

IEICE TECHNICAL REPORT Vol. 111 ( 389 ) page： 343 - 348 2012.1

Publisher：The Institute of Electronics, Information and Communication Engineers

We preliminarily constructed a software system to automatically segment cellular regions from phase-contrast microscopic imagery and then to extract the cellular shape features at high speed. Generally, it is difficult to segment cellular regions from phase-contrast microscopic images, because of halo around object and inhomogeneous background, while phase-contrast microscopy is suitable for real-time cellular monitoring. First, we proposed a segmentation method with morphological filters. Then, we devised fast algorithms to calculate cellular features based on chain-code acquired from the segmented image. Finally, we evaluated the performance of proposed method using F-value by comparing the result by the proposed method with the result obtained manually by an expert.

Image-based cell quality assessment: Modeling of cell morphology and quality for clinical cell therapy Reviewed

Sasaki Hiroto, Matsuoka Fumiko, Yamamoto Wakana, Kojima Kenji, Honda Hiroyuki, Kato Ryuji

COMPUTATIONAL MODELING IN TISSUE ENGINEERING Vol. 10 page： 207 - 226 2012.1

Novel small-caliber vascular grafts with trimeric peptide for acceleration of endothelialization. Reviewed

Kuwabara Fumiaki, Narita Yuji, Yamawaki-Ogata Aika, Kanie Kei, Kato Ryuji, Satake Makoto, Kaneko Hiroaki, Oshima Hideki, Usui Akihiko, Ueda Yuichi

ANNALS OF THORACIC SURGERY Vol. 93 ( 1 ) page： 156 - 163 2012.1

Enhancement of the Activity of a Lactobacilli-Aggregating Peptide by Freezing Treatment Reviewed

Asai Yuji, Sugita Tomoya, Kato Ryuji, Okochi Mina, Nakagawa Kyuya, Honda Hiroyuki

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN Vol. 45 ( 8 ) page： 609 - 614 2012

Screening of peptides with a high affinity to bile acids using peptide arraysand a computational analysis. Reviewed

Takeshita Toshiyuki, Okochi Mina, Kato Ryuji, Kaga Chiaki, Tomita Yasuyuki, Nagaoka Satoshi, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 112 ( 1 ) page： 92 - 97 2011.4

The ratio of adiponectinto homeostasis model assessment of insulin resistance is a powerful index ofeach component of metabolic syndrome in an aged Japanese population resultsfrom the KING Study. Reviewed

Nakatochi Masahiro, Miyata Seiko, Tanimura Daisuke, Izawa Hideo, Asano Hiroyuki, Murase Yosuke, Kato Ryuji, Ichihara Sahoko, Naruse Keiko, Matsubara Tatsuaki, Honda Hiroyuki, Yokota Mitsuhiro

DIABETES RESEARCH AND CLINICAL PRACTICE Vol. 92 ( 3 ) page： 61 - 65 2011.3

Amino acid sequence preferences to control cell-specific organization of endothelial cells, smooth muscle cells, and fibroblasts. Reviewed International journal

Kanie Kei, Kato Ryuji, Zhao Yingzi, Narita Yuji, Okochi Mina, Honda Hiroyuki

JOURNAL OF PEPTIDE RESEARCH Vol. 17 ( 6 ) page： 479 - 486 2011.2

Gingival and dermal fibroblasts their similarities anddifferences revealed from gene expression. Reviewed

Ebisawa Katsumi, Kato Ryuji, Okada Mai, Sugimura Tomotaka, Abdul Latif Mazlyzam, Hori Yusuke, Narita Yuji, Ueda Minoru, Honda Hiroyuki, Kagami Hideaki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 111 ( 3 ) page： 255 - 258 2011.2

Peptide array-based peptide-cell interaction analysis. Reviewed International journal

Kato Ryuji, Kaga Chiaki, Kanie Kei, Kunimatsu Mitoshi, Okochi Mina, Honda Hiroyuki

MINI-REVIEWS OF ORGANIC CHEMISTRY Vol. 8 ( 2 ) page： 171 - 177 2011

Screening ofpeptides with a high affinity for ZnO using spot-synthesized peptide arrays andcomputational analysis. Reviewed International journal

Okochi Mina, Ogawa Masatoshi, Kaga Chiaki, Sugita Tomoya, Tomita Yasuaki, Kato Ryuji< Honda Hiroyuki

ACTA BIOMATERIALIA Vol. 6 ( 6 ) page： 2301 - 2306 2010.12

Acompact, automated cell culture system for clinical scale cell expansion fromprimary tissues. Reviewed International journal

Kato Ryuji, Iejima Daisuke, Agata Hideki, Asahina Izumi, Okada Kunihiko, Ueda Minoru, Honda Hiroyuki, Kagami Hideaki

TISSUE ENGINEERING PART C METHODS Vol. 16 ( 5 ) page： 947 - 956 2010.11

Novel strategy for prevention ofesophageal stricture after endoscopic surgery. Reviewed International journal

Mizutani Taro, Tadauchi Akimitsu, Arinobe Manabu, Narita Yuji, Kato Ryuji, Niwa Yasumasa, Ohmiya Naoki, Itoh Akihiro, Hirooka Yoshiki, Honda Hiroyuki, Ueda Minoru, Goto Hidemi

HEPATOGASTROENTEROLOGY Vol. 57 ( 102-103 ) page： 1150 - 1156 2010.9

Practical cell labeling with magnetitecationic liposomes for cell manipulation. Reviewed International journal

Ito Hiroshi, Nonogaki Yurika, Kato Ryuji, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 110 ( 1 ) page： 124 - 129 2010.2

Directed evolution of angiotensin II-inhibiting peptides using a microbeaddisplay. Reviewed International journal

Gan Rui, Furuzawa Seiji, Kojima Takaaki, Kanie Keie, Kato Ryuji, Okochi Mina, Honda Hiroyuki, Nakano Hideo

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 109 ( 4 ) page： 411 - 417 2009.11

Magnetic manipulation device for theoptimization of cell processing conditions. Reviewed International journal

Ito Hiroshi, Kato Ryuji, Ino Kosuke, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 109 ( 2 ) page： 182 - 188 2009.8

Peptide array-based analysis of the specific IgE andIgG4 in cow's milk allergens and its use in allergy evaluation. Reviewed International journal

Matsumoto Naoki, Okochi Mina, Matsushima Miyoko, Kato Ryuji, Takase Tomokazu, Yoshida Yasuko, Kawase Mitsuo, Isobe Ken-ichi, Kawabe Tsutomu, Honda Hiroyuki

PEPTIDES Vol. 30 ( 10 ) page： 1840 - 1847 2009.7

Cancer DNA microarrayanalysis considering multi-subclass with graph-based clustering method. Reviewed International journal

Kawamura Takashi, Hutoh Hironori, Tomita Yasuyuki, Kato Ryuji, Honda Hiroyuk

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 106 ( 5 ) page： 442 - 448 2008.12

Size dependent heat generation of magnetite nanoparticles under AC magneticfield for cancer therapy. Reviewed International journal

Motoyama Jun, Hakata Toshiyuki, Kato Ryuji, Yamashita Noriyuki, Morino Tomio, Kobayashi Takeshi, Honda Hiroyuki

BIOMAGNETIC RESEARCH AND TECHNOLOGY Vol. 6 page： 4 2008.10

A motif detection and classificationmethod for peptide sequences using genetic programming. Reviewed International journal

Tomita Yasuyuki, Kato Ryuji, Okochi Mina, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 106 ( 2 ) page： 154 - 161 2008.9

Screening of anovel octamer peptide, CNSCWSKD, that induces caspase-dependent cell death. Reviewed International journal

Kaga Chiaki, Okochi Mina, Nakanishi Mari, Hayashi Hiroki, Kato Ryuji, Honda Hiroyuki

BIOCHEMICAL AND BIOPHYSICAL RESARCH COMMUNICATIONS Vol. 362 ( 4 ) page： 1063 - 1068 2008.8

Computationally assistedscreening and design of cell-interactive peptides by a cell-based assay usingpeptide arrays and a fuzzy neural network algorithm. Reviewed International journal

Kaga Chiaki, Okochi Mina, Tomoita Yasuyuki, Kato Ryuji, Honda Hiroyuki

BIOTECHNIQUES Vol. 44 ( 3 ) page： 393 - 402 2008.5

Identification of HLA-A24-restricted epitopes with high affinities to Hsp70using peptide arrays. Reviewed International journal

Okochi Mina, Hayashi Hiroki, Ito Akira, Kato Ryuji, Tamura Yasuaki, Sato Noriyuki, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 105 ( 3 ) page： 198 - 203 2008.4

Screening of cell adhesive peptide from the human laminin-5 alpha 3 chain globular 2 and 3 domains. Reviewed International journal

Okochi Mina, Nomura Shigeyuki, Kaga Chiaki, Honda Hiroyuki

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN Vol. 41 ( 3 ) page： 206 - 209 2008.3

Cryopreservation of mouse adipose tissue-derived stem/progenitorcells. Reviewed International journal

Oishi Koichi, Noguchi Hirofumi, Yukawa Hiroshi, Miyazaki Takamichi, Kato Ryuji, Kitagawa Yasuo, Ueda Minoru, Hayashi Shuji

CELL TRANSPLANTATION Vol. 17 ( 1-2 ) page： 35 - 41 2008.1

Peptide array-basedinteraction assay of solid-bound peptides and anchorage-dependant cells and itseffectiveness in cell-adhesive peptide design. Reviewed International journal

Kato Ryuji, Kaga Chiaki, Kunimatsu Mitoshi, Kobayashi Takeshi, Honda Hiroyuki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 101 ( 6 ) page： 485 - 495 2006.8

High-throughputscreening of cell death inducible short peptides from TNF-related apoptosis-inducing ligand sequence. Reviewed International journal

Okochi Mina, Nakanishi Mari, Kato Ryuji, Kobayashi Takeshi, Honda Hiroyuki

FEBS LETTERS Vol. 580 ( 3 ) page： 885 - 889 2006.1

Novel strategy for protein exploration high-throughput screening assisted withfuzzy neural network. Reviewed International journal

Kato Ryuji, Nakano Hideo, Konishi Hiroyuki, Kato Katsuya, Koga Yuchi, Yamane Tsuneo, Kobayashi Takeshi, Honda Hiroyuki

JOURNAL OF MOLECULAR BIOLOGY Vol. 351 ( 3 ) page： 683 - 692 2005.6

Angiotensin IIinhibitory peptide found in the receptor sequence using peptide array. Reviewed International journal

Kato Ryuji, Kunimatsu Mitoshi, Fujimoto Seigo, Kobayashi Takeshi, Honda Hiroyuki

BIOCHEMICAL AND BIOPHYSICAL RESARCH COMMUNICATIONS Vol. 315 ( 1 ) page： 22 - 29 2004.1

Hidden Markov model-based approach as the first screening of binding peptides that interact with MHC class II molecules. Reviewed International journal

Kato Ryuji, Noguchi Hideki, Honda Hiroyuki, Kobayashi Takeshi

ENZYME AND MICROBIAL TECHNOLOGY Vol. 33 ( 4 ) page： 472 - 481 2003.6

Hidden Markov model-based prediction of antigenic peptides that interact with MHC class II molecules. Reviewed International coauthorship International journal

Noguchi Hideki, Kato Ryuji, Hanai Taizo, Matubara Yukari, Honda Hiroyuki, Vladimir Brusic, Kobayashi Takeshi

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 94 ( 3 ) page： 264 - 270 2002.11

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Papers - KATO, Ryuji