研究者詳細 - 佐藤　義朗

ドップラーセンサを用いた新生児の心拍測定におけるテンプレートマッチング呼吸残差法の検討査読有り

上林眞司, 森智也, 佐藤義朗

電子情報通信学会論文誌B 通信 J104-B 巻 ( 3 ) 頁： 369 - 378 2021年3月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：電子情報通信学会

従来，ドップラーセンサを使い，電波により遠隔で心拍を測定する方法の研究が数多く報告されているが，呼吸・体動の影響の除去が課題であった．心拍による胸壁の振幅は，呼吸による振幅の1/10程度であり，更に心拍間隔，呼吸間隔は共に，1周期ごとにわずかに変動するため，呼吸と心拍が混在する信号波形から心拍のみを抽出し精度よく測定することは容易ではない．本研究では，体動の少ない保育器内の新生児をターゲットとし，ドップラーセンサ出力波形から呼吸のテンプレート波形を自動的に生成し，テンプレートマッチング法により1周期ごとに同期して呼吸波形を除去して心拍を測定する方法を提案する．本手法を新生児モデル実験及び成人実験に適用した結果，測定時間10秒の場合，心拍間隔の平均値の測定誤差3%程度の遠隔心拍測定が実現できることを確認した．

DOI： 10.14923/transcomj.2020jbp3039

Brain damage caused by neonatal hypoxia-ischemia and the effects of hypothermia in severe combined immunodeficient (SCID) mice. 査読有り国際誌

Ogawa Y, Tanaka E, Sato Y, Tsuji M

Experimental neurology 337 巻頁： 113577 - 113577 2021年3月

Non-invasive biosignals detection for continuous monitoring of a neonate using quartz crystal resonator 査読有り

Shiro Watanabe, Yuichi Murozaki, Hirotaka Sugiura, Yoshiaki Sato, Kazuya Honbe, Fumihito Arai

Sensors and Actuators A: Physical 317 巻頁： 112475 - 112475 2021年1月

Muse細胞を用いた新生児低酸素性虚血性脳症の新規治療法開発～動物実験から探索的臨床試験（医師主導治験）まで～招待有り査読有り

佐藤義朗

日本周産期・新生児医学会雑誌 56 巻 ( 4 ) 頁： 586 - 589 2021年

担当区分：筆頭著者,　最終著者,　責任著者記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：一般社団法人日本周産期・新生児医学会

はじめに　新生児死亡率は年々減少しているが，脳性麻痺の発症は出生1,000人に対し，1.5から2.5の間であり一向に減少の兆しがない．脳性麻痺は生涯続き，1人当たりの経済的費用は約1億円と非常に高額である．患者本人はもちろん，支える家族にとっても大きな負担となる．そのため脳性麻痺を未然に防ぐことが極めて重要である．　周産期低酸素性虚血性脳症（HIE）は，脳性麻痺の主たる原因である．発症頻度は出生1,000人に対し1.3から1.7であるが，確立した治療は低体温療法のみである．その低体温療法も18カ月後の死亡，もしくは，重度の神経学的後遺症を減少させる治療必要数（NNT：number needed to treat）は9と効果は限定的であり，新たな治療法が望まれる．そこで，我々は幹細胞療法に着目し，研究を進めてきた．

DOI： 10.34456/jjspnm.56.4_586

Metabolomic analysis and mass spectrometry imaging after neonatal stroke and cell therapies in mouse brains. 査読有り国際誌

Tanaka E, Ogawa Y, Fujii R, Shimonaka T, Sato Y, Hamazaki T, Nagamura-Inoue T, Shintaku H, Tsuji M

Scientific reports 10 巻 ( 1 ) 頁： 21881 - 21881 2020年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

Ischemic brain injury provokes complex, time-dependent downstream pathways that ultimately lead to cell death. We aimed to demonstrate the levels of a wide range of metabolites in brain lysates and their on-tissue distribution following neonatal stroke and cell therapies. Postnatal day 12 mice underwent middle cerebral artery occlusion (MCAO) and were administered 1 × 105 cells after 48 h. Metabolomic analysis of the injured hemisphere demonstrated that a variety of amino acids were significantly increased and that tricarboxylic acid cycle intermediates and some related amino acids, such as glutamate, were decreased. With the exception of the changes in citric acid, neither mesenchymal stem/stromal cells nor CD34+ cells ameliorated these changes. On-tissue visualization with matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) imaging revealed that the signal intensity of glutamate was significantly decreased in the infarct area, consistent with the metabolomic analysis, while its intensity was significantly increased in the peri-infarct area after MCAO. Although cell therapies did not ameliorate the changes in metabolites in the infarct area, mesenchymal stem cells ameliorated the increased levels of glutamate and carnitine in the peri-infarct area. MALDI-MS imaging showed the location-specific effect of cell therapies even in this subacute setting after MCAO. These methodologies may be useful for further investigation of possible treatments for ischemic brain injury.

DOI： 10.1038/s41598-020-78930-x

Mesenchymal stem/stromal cells stably transduced with an inhibitor of CC chemokine ligand 2 ameliorate bronchopulmonary dysplasia and pulmonary hypertension. 査読有り国際誌

Suzuki T, Sato Y, Yamamoto H, Kato T, Kitase Y, Ueda K, Mimatsu H, Sugiyama Y, Onoda A, Saito S, Takahashi Y, Nakayama T, Hayakawa M

Cytotherapy 22 巻 ( 4 ) 頁： 180 - 192 2020年4月

Autologous cord blood cell therapy for neonatal hypoxic-ischaemic encephalopathy: a pilot study for feasibility and safety. 査読有り国際誌

Tsuji M, Sawada M, Watabe S, Sano H, Kanai M, Tanaka E, Ohnishi S, Sato Y, Sobajima H, Hamazaki T, Mori R, Oka A, Ichiba H, Hayakawa M, Kusuda S, Tamura M, Nabetani M, Shintaku H

Scientific reports 10 巻 ( 1 ) 頁： 4603 - 4603 2020年3月

記述言語：英語掲載種別：研究論文（学術雑誌）

Neonatal hypoxic-ischaemic encephalopathy (HIE) is a serious condition; many survivors develop neurological impairments, including cerebral palsy and intellectual disability. Preclinical studies show that the systemic administration of umbilical cord blood cells (UCBCs) is beneficial for neonatal HIE. We conducted a single-arm clinical study to examine the feasibility and safety of intravenous infusion of autologous UCBCs for newborns with HIE. When a neonate was born with severe asphyxia, the UCB was collected, volume-reduced, and divided into three doses. The processed UCB was infused at 12-24, 36-48, and 60-72 hours after the birth. The designed enrolment was six newborns. All six newborns received UCBC therapy strictly adhering to the study protocol together with therapeutic hypothermia. The physiological parameters and peripheral blood parameters did not change much between pre- and postinfusion. There were no serious adverse events that might be related to cell therapy. At 30 days of age, the six infants survived without circulatory or respiratory support. At 18 months of age, neurofunctional development was normal without any impairment in four infants and delayed with cerebral palsy in two infants. This pilot study shows that autologous UCBC therapy is feasible and safe.

DOI： 10.1038/s41598-020-61311-9

Comparison of Clinical Characteristics of Human Metapneumovirus and Respiratory Syncytial Virus Infections in Hospitalized Young Children.

A Novel Treatment with Stem Cells from Human Exfoliated Deciduous Teeth for Hypoxic-Ischemic Encephalopathy in Neonatal Rats. 査読有り国際誌

Kitase Y, Sato Y, Ueda K, Suzuki T, Mikrogeorgiou A, Sugiyama Y, Matsubara K, Tsukagoshi Okabe Y, Shimizu S, Hirata H, Yukawa H, Baba Y, Tsuji M, Takahashi Y, Yamamoto A, Hayakawa M

Stem cells and development 29 巻 ( 2 ) 頁： 63 - 74 2020年1月

Establishment of a Novel Fetal Growth Restriction Model and Development of a Stem-Cell Therapy Using Umbilical Cord-Derived Mesenchymal Stromal Cells. 査読有り国際誌

Kitase Y, Sato Y, Arai S, Onoda A, Ueda K, Go S, Mimatsu H, Jabary M, Suzuki T, Ito M, Saito A, Hirakawa A, Mukai T, Nagamura-Inoue T, Takahashi Y, Tsuji M, Hayakawa M

Frontiers in cellular neuroscience 14 巻頁： 212 - 212 2020年

Risk factors for absence of catch-up growth in small for gestational age very low-birthweight infants. 査読有り国際誌

Arai S, Sato Y, Muramatsu H, Yamamoto H, Aoki F, Okai Y, Kataoka S, Hanada Y, Hamada M, Morimoto Y, Kojima S, Natsume J, Takahashi Y, Nagoya Collaborative Clinical Research Team

Pediatrics international : official journal of the Japan Pediatric Society 61 巻 ( 9 ) 頁： 889 - 894 2019年9月

Taniguchi A, Kawada JI, Go K, Fujishiro N, Hosokawa Y, Maki Y, Sugiyama Y, Suzuki M, Tsuji T, Hoshino S, Muramatsu H, Kidokoro H, Kinoshita F, Hirakawa A, Takahashi Y, Sato Y, Natsume J, Nagoya Collaborative Clinical Research Team

Japanese journal of infectious diseases 72 巻 ( 4 ) 頁： 237 - 242 2019年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会

Human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) are the leading causes of acute respiratory tract infection in children, and clinical manifestations of these virus infections are considered similar. To investigate the differences in clinical characteristics between HMPV and RSV infections in young children, we prospectively enrolled children ＜ 3 years old who required hospitalization with acute respiratory tract infection due to HMPV or RSV at 10 hospitals in Japan. We enrolled 48 children with HMPV infection and 141 with RSV infection. Patients with HMPV infection were older than those with RSV infection. High-grade fever was more frequently observed in patients with HMPV infection, whereas no significant differences in respiratory symptoms were apparent. Abnormal serum lactate dehydrogenase values and consolidation shadows on chest X-ray were more frequently observed in patients with HMPV infection. During hospitalization, nasal mucus suction was more frequently required in patients with RSV infection. On the other hand, β2-adrenergic agonists, corticosteroids, and leukotriene receptor antagonists were more frequently used in patients with HMPV infection. These findings suggest that HMPV and RSV infections show similar respiratory symptoms, but HMPV infection is more likely to lead to the development of pneumonia, at least among hospitalized young children.

DOI： 10.7883/yoken.JJID.2018.480

Factors related to survival discharge in trisomy 18: A retrospective multicenter study. 査読有り国際誌

Kato E, Kitase Y, Tachibana T, Hattori T, Saito A, Muramatsu Y, Takemoto K, Yamamoto H, Hayashi S, Yasuda A, Kato Y, Ieda K, Oshiro M, Sato Y, Hayakawa M

American journal of medical genetics. Part A 179 巻 ( 7 ) 頁： 1253 - 1259 2019年7月

Design of a prospective multicenter randomized controlled trial evaluating the effects of gastric lavage on coffee-ground emesis in neonates: study protocol. 査読有り

Maeda T, Sato Y, Hirakawa A, Nakatochi M, Kinoshita F, Suzuki T, Ichimura S, Ito R, Kudo R, Suzuki M, Hoshino S, Sugiyama Y, Muramatsu H, Kidokoro H, Kawada JI, Takahashi Y, Nagoya Collaborative Clinical Research Team

Nagoya journal of medical science 81 巻 ( 2 ) 頁： 227 - 232 2019年5月

Comparison of high-dose and low-dose corticosteroid therapy for refractory Mycoplasma pneumoniae pneumonia in children. 査読有り国際誌

Okumura T, Kawada JI, Tanaka M, Narita K, Ishiguro T, Hirayama Y, Narahara S, Tsuji G, Sugiyama Y, Suzuki M, Tsuji T, Hoshino S, Nakatochi M, Muramatsu H, Kidokoro H, Takahashi Y, Sato Y, Nagoya Collaborative Clinical Research Team

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 25 巻 ( 5 ) 頁： 346 - 350 2019年5月

Correction: Treatment with silver nitrate versus topical steroid treatment for umbilical granuloma: A non-inferiority randomized control trial. 査読有り国際誌

Chikako Ogawa, Yoshiaki Sato, Chiyo Suzuki, Azusa Mano, Atsushi Tashiro, Takafumi Niwa, Sayako Hamazaki, Yoshihiro Tanahashi, Midori Suzumura, Satoshi Hayano, Masahiro Hayakawa, Takeshi Tsuji, Shin Hoshino, Yuichiro Sugiyama, Hiroyuki Kidokoro, Jun-Ichi Kawada, Hideki Muramatsu, Akihiro Hirakawa, Masahiko Ando, Jun Natsume, Seiji Kojima

PloS one 14 巻 ( 6 ) 頁： e0218205 2019年

Magnetic resonance spectroscopy in preterm infants: association with neurodevelopmental outcomes. 査読有り国際誌

Hyodo R, Sato Y, Ito M, Sugiyama Y, Ogawa C, Kawai H, Nakane T, Saito A, Hirakawa A, Kidokoro H, Natsume J, Hayakawa M

Archives of disease in childhood. Fetal and neonatal edition 103 巻 ( 3 ) 頁： F238 - F244 2018年5月

Successful Infant Pneumonectomy with Unilateral Pulmonary Artery Occlusion Test. 査読有り

Kato K, Kato T, Hayano S, Fukasawa Y, Numaguchi A, Hattori T, Saito A, Sato Y, Hayakawa M

International heart journal 59 巻 ( 1 ) 頁： 237 - 239 2018年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：一般社団法人インターナショナル・ハート・ジャーナル刊行会

The use of unilateral pulmonary artery occlusion (UPAO) test for the preoperative evaluation of pneumonectomy was reported in adult patients. On the contrary, in infants, no strategies have yet been recommended to predict hemodynamics after pneumonectomy, nor has use of the UPAO test been reported. We describe the first case of infant with abnormal pulmonary circulation in whom successful pneumonectomy was performed after preoperative evaluation using UPAO test. Right pneumonectomy was planned for an 8-month-old girl, because of decreased right pulmonary function, high risk of pneumothorax, and impaired left lung expansion due to overexpansion caused by severe left bronchial stenosis and bronchomalacia. However, she had also prolonged pulmonary hypertension and there was difficulty in accurate echocardiographic evaluation of its severity due to concomitant left pulmonary artery stenosis. Furthermore, contrast-enhanced computer tomography suggested a certain degree of right pulmonary venous flow, discordant with the result showing scarce right pulmonary flow in perfusion scintigraphy. Predicting postoperative hemodynamic changes was therefore considered difficult. To evaluate these concerns, we performed cardiac catheterization and UPAO test to simulate postoperative hemodynamics. Pulmonary arteriography showed decreased but significant right pulmonary arterial and venous flows. Measurements including pulmonary artery pressure and cardiac index showed no marked changes after occlusion. Based on UPAO test results, the operation was successfully performed and hemodynamics remained stable postoperatively. The UPAO test may be useful for infants with cardiopulmonary impairment to evaluate the tolerability of pneumonectomy.

DOI： 10.1536/ihj.16-606