論文 - 西口　康二

Defects in RGS9 or its anchor protein R9AP in patients with slow photoreceptor deactivation. 査読有り

Nature   427 巻 ( 6969 ) 頁： 75-78   2004年

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Novel mutations in the cellular retinaldehyde-binding protein gene (RLBP1) associated with retinitis punctata albescens: evidence of interfamilial genetic heterogeneity and fundus changes in heterozygotes 査読有り

Fishman GA, Roberts MF, Derlacki DJ, Grimsby JL, Yamamoto H, Sharon D, Nishiguchi KM, Dryja TP

Arch Ophthalmol   122 巻 ( 1 ) 頁： 70-75   2004年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Mutation screening of the phosducin gene PDC in patients with retinitis pigmentosa and allied diseases 査読有り

Mol Vis   10 巻   頁： 62-64   2004年

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

A novel mutation (I143NT) in guanylate cyclase-activating protein 1 (GCAP1) associated with autosomal dominant cone degeneration 査読有り

  45 巻   頁： 3863-3870   2004年

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Recessive NRL mutations in patients with clumped pigmentary retinal degeneration and relative preservation of blue cone function 査読有り

Nishiguchi KM, Friedman JS, Sandberg MA, Swaroop A, Berson EL, Dryja TP

  101 巻   頁： 17819-17824   2004年

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Cone cGMP-gated channel mutations and clinical findings in patients with achromatopsia, macular degeneration, and other hereditary cone diseases 査読有り

  25 巻   頁： 248-258   2005年

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Recessive mutations in the CYP4V2 gene in East Asian and Middle Eastern patients with Bietti crystalline corneoretinal dystrophy 査読有り

Lin J, Nishiguchi KM, Nakamura M, Dryja TP, Berson EL, Miyake Y

J Med Genet   42 巻   2005年

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Retinopathy mutations in the bZIP protein NRL alter phosphorylation and transcriptional activity 査読有り

Hum Mutat   28 巻   頁： 589-598   2007年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

*The role of VEGF and VEGFR2/Flk1 in proliferation of retinal progenitor cells in murine retinal degeneration 査読有り

  48 巻   頁： 4315-4320   2007年

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

*Identification of photoreceptor precursors in the pars plana during ocular development and after retinal injury 査読有り

Nishiguchi KM, Nakamura M, Kaneko H, Kachi S, Terasaki H

Invest Ophthalmol Vis Sci   49 巻   頁： 422-428   2008年1月

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

*Characteristics of Bone Marrow-Derived Microglia in the Normal and Injured Retina 査読有り

Kaneko H, Nishiguchi KM, Nakamura M, Kachi S, Terasaki H

  49 巻   頁： 4162-4168   2008年9月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

*Retardation of Photoreceptor Degeneration in the Detached Retina of rd1 Mouse 査読有り

Kaneko H, Nishiguchi KM, Nakamura M, Kachi S, Terasaki H

Invest Ophthalmol Vis Sci   49 巻   頁： 781-787   2008年2月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

TRPM1 mutations are associated with the complete form of congenital stationary night blindness 査読有り

Nakamura M, Sanuki R, Yasuma TR, Onishi A, Nishiguchi KM, Koike C, Kadowaki M, Kondo M, Miyake Y, Furukawa T.

Mol Vis   16 巻   頁： 425-437   2010年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Regulation of pathologic retinal angiogenesis and inhibition of VEGF-VEGFR2 binding by soluble heparan sulfate. 査読有り

Nishiguchi KM, Kataoka K, Kachi S, Komeima K, Terasaki H

PLoS ONE   5 巻   頁： e13493   2010年

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

The roles of vitreal macrophages and circulating leukocytes in retinal neovascularization. 査読有り

Kataoka K, Nishiguchi KM, Kaneko H, van Rooijen N, Kachi S, Terasaki H

Invest Ophthalmol Vis Sci   in press 巻   2010年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

EVALUATION OF RETINAL ARTERIOLES IN RETINITIS PIGMENTOSA: Arterial Lumen Diameter Reduced With Retinal Degeneration and Wall Thickness Related to Systemic Condition.

Ota J, Inooka T, Tomita R, Kominami T, Koyanagi Y, Ito Y, Terasaki H, Nishiguchi KM, Ueno S

Retina (Philadelphia, Pa.)   45 巻 ( 3 ) 頁： 532 - 540   2025年3月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Retina  

Purpose:Retinal arterioles are attenuated in eyes with retinitis pigmentosa, but dimensions such as lumen diameter and wall thickness have not been thoroughly investigated. The aim of the study was to determine the dimensions of reduced retinal arterioles in eyes with retinitis pigmentosa.Methods:The study included 66 patients with retinitis pigmentosa (66 eyes) and 36 control participants (36 eyes) for whom adaptive optics recordings of the retinal arteries were available. Lumen diameter and wall thickness were measured on the adaptive optics images. The correlations between arteriole dimensions and clinical parameters were determined.Results:The mean lumen diameter was significantly smaller in patients with retinitis pigmentosa than in controls, while the mean wall thickness did not differ significantly. Lumen diameter was significantly and positively correlated with the mean deviation of the Humphrey 10-2 field analyzer and ellipsoid zone width. The wall thickness and wall-to-lumen ratio were significantly and positively correlated with age and systolic blood pressure but not with mean deviation and ellipsoid zone width.Conclusion:Retinal arteriole dimensions were significantly correlated with retinal degeneration and systemic factors.

DOI： 10.1097/IAE.0000000000004333

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PubMed

Plate Manufacturing Constraint in Topology Optimization Using Anisotropic Filter

Wada, Y; Shimada, T; Nishiguchi, K; Okazawa, S; Tsubokura, M

COMPUTER-AIDED DESIGN   180 巻   2025年3月

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出版者・発行元：CAD Computer Aided Design  

The manufacturing constraint of topology optimization for finding structures composed primarily of plate cross sections with an automatically decided surface normal is discussed. The anisotropic partial differential equation filter for the sensitivity of the objective function is designed such that the eigenvalue-resolved stress tensor is converted to the anisotropic filter tensor. The user can obtain an optimal shape proposal with different degrees of plate formation by changing the influence radius of the ellipsoidal filter. Plate structure formation and stiffness compliance performance are discussed through three sample problems: a solid bar subjected to torsional loads, an L-shaped member subjected to multiple loads, and a vehicle body frame intended to be a complex frame. The finite element analysis with 3–300 million degrees of freedom is required to form a shell plate structure from a large ingot with a volume fraction constraint of 1%–5%. A voxel topology optimization software that utilizes the building cube method framework available in massively parallel environments is developed, and the parallel performance of the optimization routine, including the plate filtering, is measured.

DOI： 10.1016/j.cad.2024.103823

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Silicone oil, an intraocular surgical adjuvant, induces retinal ferroptosis

Shimizu, H; Tanaka, H; Tazaki, A; Yamada, K; Suzumura, A; Ota, J; Ushio-Watanabe, N; Zheng, H; Kataoka, K; Hara, H; Nishikawa, Y; Yasukawa, T; Suzuma, K; Terasaki, H; Nishiguchi, KM; Kato, M; Toyokuni, S; Kaneko, H

FREE RADICAL BIOLOGY AND MEDICINE   228 巻   頁： 33 - 43   2025年2月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Free Radical Biology and Medicine  

Vitrectomy with silicone oil (SO) endotamponade is an effective treatment for vision-threatening retinal diseases. However, unexplained vision impairment has been reportedly critical side effects. Previously, we reported that the eyes with ocular toxoplasmosis showed retinal ferroptosis with the clinical sign of reduced intravitreal iron (Fe). We also found that total iron levels in sub-silicone oil fluid (SOF) in eyes with SO endotamponade were significantly reduced. We hypothesized that the cause of complications related to SO endotamponade is retinal ferroptosis and that low total iron in SOF is a secondary change that occurs similarly to the changes in ocular toxoplasmosis. In this study, we measured total iron levels in ocular fluid from patients, rabbits with SO endotamponade. Retinal iron taken up from the SOF was evaluated using laser ablation inductively coupled plasma mass spectrometry in human and rabbit eyes. Retinal ferroptosis was confirmed by immunohistochemistry of 4-hydrox-2-nonenal-modified proteins, FeRhoNox-1 staining, western blotting and RT-PCR. We found low total iron levels in the SOF, increased oxidative stress and Fe uptake from the SOF into the retinae of human and rabbit eyes, as well as decreased GPx4 expression, increased FeRhoNox-1 signals and altered Fe-related gene expression in SO-filled rabbit eyes. Of note, the target of ferroptosis was Müller cells. We generated an in vitro silicone oil-filled eye model using MIO-M1 cells (a human Müller cell line). The in vitro SO-filled eye model showed decreased GPx4 expression and increased intracellular catalytic Fe(II), an increase in ferroptosis, prevention of cell death by ferrostatin-1, a ferroptosis inhibitor, and altered Fe-related gene expression. These results indicate that the cause of complications related to SO endotamponade was the induction of retinal (Müller cell) ferroptosis, which can be prevented by ferrostatin-1.

DOI： 10.1016/j.freeradbiomed.2024.12.039

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Classification of fundus autofluorescence images based on macular function in retinitis pigmentosa using convolutional neural networks

Kominami, T; Ueno, S; Ota, J; Inooka, T; Oda, M; Mori, K; Nishiguchi, KM

JAPANESE JOURNAL OF OPHTHALMOLOGY     2025年2月

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記述言語：英語  

DOI： 10.1007/s10384-025-01163-w

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Phenotypic Distinctions Between EYS- and USH2A-Associated Retinitis Pigmentosa in an Asian Population.

Yeo EYH, Kominami T, Tan TE, Babu L, Ong KGS, Tan W, Bylstra YM, Jain K, Tang RWC, Farooqui SZ, Kam SPR, Chan CM, Mathur RS, Jamuar SS, Lim WK, Nishiguchi K, Fenner BJ

Translational vision science & technology   14 巻 ( 2 ) 頁： 16   2025年2月

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記述言語：英語   出版者・発行元：Translational vision science & technology  

Purpose: This study compares clinical characteristics of retinitis pigmentosa (RP) associated with mutations in the EYS and USH2A genes in a Southeast Asian cohort. Methods: Prospective single-center study of families with EYS- or USH2A-associated RP seen at the Singapore National Eye Centre. Comprehensive ophthalmic evaluations, multimodal imaging, genetic testing, and longitudinal follow-up identified clinically useful differentiating features between the two genotypes. Results: A total of 300 families with RP were enrolled, with EYS- and USH2A-associated RP, accounting for 24.7% of all probands and 50.7% of solved or likely solved cases. USH2A cases were predominantly nonsyndromic RP (75%). EYS-associated RP was more severe in functional and structural outcomes, and patients were more myopic than USH2A (SE -3.31 vs. -0.69; P < 0.0001). EYS RP displayed peripapillary nasal sparing on autofluorescence imaging more frequently than USH2A (57.6% vs. 26.7%; P = 0.006), whereas USH2A cases more often had a parafoveal ring (73.3% vs. 30.3%; P = 0.0002). Multiple logistic regression identified diagnostic features with 83.2% accuracy in distinguishing between EYS and USH2A, validated in a second unrelated clinical cohort. Conclusions: EYS- and USH2A-associated RP have overlapping clinical presentations but can often be distinguished based on a constellation of phenotypic features including disease onset and severity, refractive error, and fundus autofluorescence. These diagnostic features may support a more effective diagnostic strategy for these common forms of RP. Translational Relevance: Distinct clinical features differentiating EYS- and USH2A-associated RP provide valuable diagnostic tools that may inform personalized management and facilitate targeted interventions in clinical practice.

DOI： 10.1167/tvst.14.2.16

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Fundus autofluorescence features specific for EYS-associated retinitis pigmentosa. 国際誌

Kominami T, Tan TE, Ushida H, Jain K, Goto K, Bylstra YM, Sajiki AF, Mathur RS, Ota J, Lim WK, Nishiguchi KM, Fenner BJ

PloS one   20 巻 ( 2 ) 頁： e0318857   2025年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1371/journal.pone.0318857

PubMed

Analysis of the aqueous humor before and after the administration of faricimab in patients with nAMD

Nonogaki, R; Ota, H; Takeuchi, J; Nakano, Y; Sajiki, AF; Todoroki, T; Nakamura, K; Kaneko, H; Nishiguchi, KM

SCIENTIFIC REPORTS   14 巻 ( 1 ) 頁： 31951   2024年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Scientific Reports  

This study aimed to evaluate the changes in cytokine levels in the aqueous humor and factors of treatment resistance following intravitreal faricimab injection in treatment-naïve patients with neovascular age-related macular degeneration. A total of 32 eyes were analyzed before and after a single faricimab injection. Although the best-corrected visual acuity (BCVA) showed no significant improvement, the mean central retinal thickness decreased significantly by 73.7% (P < 0.01), and more than 90% of the eyes showed improvement in exudative changes 1 month after faricimab injection. Moreover, the aqueous humor concentrations of vascular endothelial growth factor (VEGF)-A, angiopoietin (Ang)-2, and placental growth factor considerably decreased 1 month after faricimab injection. Multivariate analyses adjusted for age, sex, BCVA, central choroidal thickness, and aqueous humor cytokines revealed that higher Ang-2 levels in the aqueous humor at baseline were associated with better treatment response to faricimab injection. These findings suggest that the dual inhibition of VEGF-A and Ang-2 by faricimab is effective in reducing exudative changes and that Ang-2 may serve as a potential biomarker for predicting faricimab treatment response.

DOI： 10.1038/s41598-024-83473-6

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その他リンク： https://www.nature.com/articles/s41598-024-83473-6

Assessment of factors affecting anterior chamber depth from data obtained from health checkup participants in Japan

Inooka, T; Kominami, T; Tomita, R; Suzumura, A; Matsuno, T; Ota, J; Koyanagi, Y; Takeyama, H; Ueno, S; Ito, Y; Nishiguchi, KM; Yuki, K

SCIENTIFIC REPORTS   14 巻 ( 1 ) 頁： 30342   2024年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Scientific Reports  

Measurement of anterior chamber depth (ACD), an important marker for the screening of primary angle-closure glaucoma, requires biometry, which is not readily used. This study assessed the relationship between ACD and health check-up data findings from participants with good corrected visual acuity in Japan. Participants underwent ophthalmic, anthropometric, and hematological assessments. The mean ACD of all 3060 participants was 3.33 ± 0.34 mm [2.22–4.72 mm]. Multivariable linear regression analysis was performed to determine factors that were significantly correlated with ACD, and logistic regression analysis was performed to predict ACD < 2.70 mm. Multivariable linear regression analysis showed that age, sex, intraocular pressure, spherical equivalent refractive error (SER), height, and fasting blood sugar levels significantly correlated with ACD (P < 0.05). Logistic regression analysis showed that age, sex, and SER were the best predictors of ACD < 2.70 mm. The area under receiving operator characteristic curves of ‘age and SER’ and ‘age, SER, and sex’ were 0.821 and 0.835, respectively, with no significant difference (P = 0.122). In conclusion, ACD correlates with several parameters, and age and SER may be particularly important for predicting ACD in participants undergoing health checkups.

DOI： 10.1038/s41598-024-82096-1

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その他リンク： https://www.nature.com/articles/s41598-024-82096-1

Clinical utility of swept-source optical coherence tomography angiography for the diagnosis of exudative maculopathy

Sajiki, A; Kataoka, K; Takeuchi, J; Ota, H; Nakano, Y; Horiguchi, E; Kaneko, H; Terasaki, H; Ito, Y; Nishiguchi, KM

JAPANESE JOURNAL OF OPHTHALMOLOGY   68 巻 ( 6 ) 頁： 614 - 620   2024年11月

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記述言語：英語   出版者・発行元：Japanese Journal of Ophthalmology  

Purpose: To assess the feasibility of swept-source optical coherence tomography angiography (SS-OCTA) to differentiate macular diseases, including nonpolypoidal macular neovascularization (MNV), polypoidal choroidal vasculopathy (PCV), type 3 MNV, and chronic central serous chorioretinopathy (CSC) without indocyanine green angiography (ICGA). Study design: Retrospective observational study. Methods: This study examined 63 eyes of 63 patients with treatment-naive neovascular age-related macular degeneration (AMD), including 23 eyes with nonpolypoidal MNV, 17 eyes with PCV, and 1 eye with type 3 MNV and 22 eyes with chronic CSC. Two independent retina specialists, blinded to the clinical diagnosis, assessed each case of neovascular AMD and chronic CSC using only B-scan and en face images of SS-OCTA without referring to other examination outcomes. Results: By SS-OCTA alone, 19 eyes were diagnosed with nonpolypoidal MNV, 17 eyes with PCV, 2 eyes with type 3 MNV, and 22 eyes with chronic CSC, indicating high sensitivity (82.6%, 94.1%, 100%, and 100%, respectively) and specificity (100%, 97.8%, 98.4%, and 100%, respectively); however, three eyes could not be diagnosed because of obscure images. The agreement of diagnosis with SS-OCTA alone was high between the two specialists (κ = 0.82). Conclusion: SS-OCTA showed high sensitivity and specificity in the differentiation of nonpolypoidal MNV, PCV, type 3 MNV, and chronic CSC. The differential criteria based on SS-OCTA could be a substitute for the ICGA-based diagnoses.

DOI： 10.1007/s10384-024-01115-w

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Genetic Risk Stratification of Primary Open-Angle Glaucoma in Japanese Individuals

Akiyama M., Tamiya G., Fujiwara K., Shiga Y., Yokoyama Y., Hashimoto K., Sato M., Sato K., Narita A., Hashimoto S., Ueda E., Furuta Y., Hata J., Miyake M., Ikeda H.O., Suda K., Numa S., Mori Y., Morino K., Murakami Y., Shimokawa S., Nakamura S., Yawata N., Fujisawa K., Yamana S., Mori K., Ikeda Y., Miyata K., Mori K., Ogino K., Koyanagi Y., Kamatani Y., Matsuda K., Yamanashi Y., Furukawa Y., Morisaki T., Okada Y., Murakami Y., Muto K., Nagai A., Nakamura Y., Obara W., Yamaji K., Takahashi K., Asai S., Takahashi Y., Higashiue S., Kobayashi S., Yamaguchi H., Nagata Y., Wakita S., Nito C., Iwasaki Y.K., Murayama S., Yoshimori K., Miki Y., Obata D., Higashiyama M., Masumoto A., Koga Y., Koretsune Y., Ninomiya T., Sonoda K.H., Nakazawa T., Aihara M., Sakata R., Kashiwagi K., Mabuchi F., Kawase K., Iwata T., Tsujikawa M., Nishiguchi K.M.

Ophthalmology   131 巻 ( 11 ) 頁： 1271 - 1280   2024年11月

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出版者・発行元：Ophthalmology  

Purpose: To assess the impact of genetic risk estimation for primary open-angle glaucoma (POAG) in Japanese individuals. Design: Cross-sectional analysis. Participants: Genetic risk scores (GRSs) were constructed based on a genome-wide association study (GWAS) of POAG in Japanese people. A total of 3625 Japanese individuals, including 1191 patients and 2434 controls (Japanese Tohoku), were used for the model selection. We also evaluated the discriminative accuracy of constructed GRSs in a dataset comprising 1034 patients and 1147 controls (the Japan Glaucoma Society Omics Group [JGS-OG] and the Genomic Research Committee of the Japanese Ophthalmological Society [GRC-JOS]) and 1900 participants from a population-based study (Hisayama Study). Methods: We evaluated 2 types of GRSs: polygenic risk scores using the pruning and thresholding procedure and a GRS using variants associated with POAG in the GWAS of the International Glaucoma Genetics Consortium (IGGC). We selected the model with the highest areas under the receiver operating characteristic curve (AUC). In the population-based study, we evaluated the correlations between GRS and ocular measurements. Main Outcome Measure: Proportion of patients with POAG after stratification according to the GRS. Results: We found that a GRS using 98 variants, which showed genome-wide significance in the IGGC, showed the best discriminative accuracy (AUC, 0.65). In the Japanese Tohoku, the proportion of patients with POAG in the top 10% individuals was significantly higher than that in the lowest 10% (odds ratio [OR], 6.15; 95% confidence interval [CI], 4.35–8.71). In the JGS-OG and GRC-JOS, we confirmed similar impact of POAG GRS (AUC, 0.64; OR [top vs. bottom decile], 5.81; 95% CI, 3.79–9.01). In the population-based study, POAG prevalence was significantly higher in the top 20% individuals of the GRS compared with the bottom 20% (9.2% vs. 5.0%). However, the discriminative accuracy was low (AUC, 0.56). The POAG GRS was correlated positively with intraocular pressure (r = 0.08: P = 4.0 × 10–4) and vertical cup-to-disc ratio (r = 0.11; P = 4.0 × 10–6). Conclusions: The GRS showed moderate discriminative accuracy for POAG in the Japanese population. However, risk stratification in the general population showed relatively weak discriminative performance. Financial Disclosure(s): Proprietary or commercial disclosure may be found in the Footnotes and Disclosures at the end of this article.

DOI： 10.1016/j.ophtha.2024.05.026

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Five-year outcomes of treat and extend regimen using intravitreal aflibercept injection for treatment-naïve age-related macular degeneration

Ota, H; Kataoka, K; Asai, K; Takeuchi, J; Nakano, Y; Nakamura, K; Todoroki, T; Nishiguchi, KM

GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY   262 巻 ( 11 ) 頁： 3483 - 3491   2024年11月

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記述言語：英語   出版者・発行元：Graefe's Archive for Clinical and Experimental Ophthalmology  

Purpose: This study evaluated the long-term outcomes of eyes with neovascular age-related macular degeneration (nAMD) treated with aflibercept according to a treat-and-extend (T&E) regimen for up to 5 years. Methods This retrospective study included 112 eyes of 111 patients with nAMD who received aflibercept according to the T&E regimen. The patients received 3 monthly injections of aflibercept followed by a T&E regimen for at least 12 months. Data, including best-corrected visual acuity (BCVA), treatment interval, presence of exudation, central retinal thickness, and central choroidal thickness were analyzed. Results: Of the 112 consecutive eyes, 66 completed the 5-year follow-up. After 5 years of treatment, BCVA (logMAR) was significantly better than baseline (0.29 ± 0.31 at baseline and 0.18 ± 0.23 at 5 years, P < 0.01). A mean of 7.0 ± 1.5 injections in the first year and 4.9 ± 2.2 injections per year thereafter were required. In eyes with subretinal hyperreflective material (SHRM) at baseline, BCVA at baseline and 5 years were significantly worse than in eyes without SHRM at baseline and 5 years. However, the eyes with SHRM required fewer injections and exhibited greater BCVA improvement. Conclusion: This retrospective study demonstrated the effectiveness of the T&E regimen with aflibercept in managing nAMD over a 5-year period, maintaining significant improvements in BCVA.

DOI： 10.1007/s00417-024-06519-5

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Phenotypic variability of <i>RP1</i>-related inherited retinal dystrophy associated with the c.5797 C > T (p.Arg1933*) variant in the Japanese population 国際誌

Natsume, K; Kominami, T; Goto, K; Koyanagi, Y; Inooka, T; Ota, J; Kawano, K; Yamada, K; Okuda, D; Yuki, K; Nishiguchi, KM; Ushida, H

SCIENTIFIC REPORTS   14 巻 ( 1 ) 頁： 25669 - 25669   2024年10月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Scientific Reports  

The phenotypes of RP1-related inherited retinal dystrophies (RP1-IRD), causing autosomal dominant (AD) and autosomal recessive (AR) diseases, vary depending on specific RP1 variants. A common nonsense mutation near the C-terminus, c.5797 C > T (p.Arg1933*), is associated with RP1-IRD, but the exact role of this mutation in genotype-phenotype correlation remains unclear. In this study, we retrospectively analyzed patients with RP1-IRD (N = 42) from a single center in Japan. AR RP1-IRD patients with the c.5797 C > T mutation (N = 14) mostly displayed macular dystrophy but rarely retinitis pigmentosa or cone-rod dystrophy. Conversely, AR RP1-IRD patients without the c.5797 C > T mutation, including those with other pathogenic RP1 variants, were mostly diagnosed with severe retinitis pigmentosa. Full-field electroretinograms were significantly better in patients homozygous or compound heterozygous for the c.5797 C > T mutation than in those without this mutation, corresponding to their milder phenotypes. Clinical tests also revealed a slower onset of age and a better mean deviation value with the static visual field in AR RP1-IRD patients with the c.5797 C > T mutation compared to those without. Therefore, the presence of c.5797 C > T may partly account for the phenotypic variety of RP1-IRD and may yield milder phenotypes. These findings may be useful for predicting the prognosis of RP1-IRD patients.

DOI： 10.1038/s41598-024-77441-3

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今月の表紙 網膜色素変性患者の前房内水晶体脱臼

前原 紘基, 西口 康二

臨床眼科   78 巻 ( 10 ) 頁： 1186 - 1186   2024年10月

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出版者・発行元：株式会社医学書院  

DOI： 10.11477/mf.1410215289

CiNii Research

A Novel Approach To Predict Glaucomatous Impairment in the Central 10° Visual Field, Excluding the Effect of Cataract

Tomita, R; Asaoka, R; Hirasawa, K; Fujino, Y; Omura, T; Inatomi, T; Obana, A; Nishiguchi, KM; Tanito, M

TRANSLATIONAL VISION SCIENCE & TECHNOLOGY   13 巻 ( 10 ) 頁： 35   2024年10月

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記述言語：英語   出版者・発行元：Translational Vision Science and Technology  

Purpose: Our previous study predicted genuine glaucomatous visual field (VF) impairment in the central 10° VF, excluding the effect of cataract, using visual acuity (VA) and global indexes of VF more accurately than pattern deviation (PD). This study aimed to improve the accuracy by using pointwise total deviation (TD) values with the machine-learning method of random forest model (RFM) and to investigate whether incorporating optical coherence tomography–measured ganglion cell-inner plexiform layer (GCIPL) thickness is useful. Methods: This retrospective study included 89 eyes with open-angle glaucoma that underwent successful cataract surgery (with or without iStent implantation or ab interno trabeculotomy). Postoperative TD in each of the 68 VF points was predicted using preoperative (1) PD, (2) VA and VF with a linear regression model (LM), and (3) VA and VF with RFM, and averaged as predicted mean TD (mTDpost). Further prediction was made by incorporating the preoperative GCIPL into the best model. Results: The mean absolute error (MAE) between the actual and predicted mTDpost with RFM (1.25 ± 1.03 dB) was significantly smaller than that with PD (3.20 ± 4.06 dB, p < 0.01) and LM (1.42 ± 1.06 dB, p < 0.05). The MAEs with the model incorporating GCIPL into RFM (1.24 ± 1.04 dB) and RFM were not significantly different. Conclusions: Accurate prediction of genuine glaucomatous VF impairment was achieved using pointwise TD with RFM. No merit was observed by incorporating the GCIPL into this model. Translational Relevance: This pointwise RFM could clinically reduce cataract effect on VF.

DOI： 10.1167/tvst.13.10.35

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GENETIC ETIOLOGY AND CLINICAL FEATURES OF ACHROMATOPSIA IN JAPAN 国際誌

Inooka, T; Hayashi, T; Tsunoda, K; Kuniyoshi, K; Kondo, H; Mizobuchi, K; Suga, A; Iwata, T; Yoshitake, K; Kondo, M; Goto, K; Ota, J; Kominami, T; Nishiguchi, KM; Ueno, S

RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES   44 巻 ( 10 ) 頁： 1836 - 1844   2024年10月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Retina  

Purpose:To ascertain the characteristics of achromatopsia (ACHM) in Japan by analyzing the genetic and phenotypic features of patients with ACHM.Methods:The medical records of 52 patients from 47 Japanese families who were clinically diagnosed with ACHM were reviewed in this retrospective observational study.Results:Thirty-six causative variants of ACHM were identified in 26 families via whole-exome sequencing: PDE6C (12 families), CNGA3 (10 families), CNGB3 (two families), and GNAT2 (two families). However, none of the 6 causative variants that are known to cause ACHM, or the 275 other genes listed in RetNet, were observed in 19 families. A significant trend toward older age and worsening of ellipsoid zone disruption on optical coherence tomography images was observed (P < 0.01). Progressive ellipsoid zone disruptions were observed in 13 eyes of seven patients during the follow-up visits. These patients harbored one or more variants in PDE6C.Conclusion:The ACHM phenotype observed in this study was similar to those observed in previous reports; however, the causative gene variants differed from those in Europe. The low identification ratio of causative genes in whole-exome sequencing suggests the presence of unique hotspots in Japanese patients with ACHM that were not detectable via ordinal whole-exome sequencing.

DOI： 10.1097/IAE.0000000000004170

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Aqueous Humor Cytokine Analysis in Age-Related Macular Degeneration After Switching From Aflibercept to Faricimab

Todoroki, T; Takeuchi, J; Ota, H; Nakano, Y; Sajiki, A; Nakamura, K; Kaneko, H; Nishiguchi, KM

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE   65 巻 ( 11 ) 頁： 15 - 15   2024年9月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Investigative Ophthalmology and Visual Science  

PURPOSE. To examine the changes in aqueous humor cytokine levels and clinical outcomes of switching from aflibercept to faricimab in eyes with neovascular age-related macular degeneration (nAMD). METHODS. Fifty-four eyes of 54 patients with AMD undergoing treatment with aflibercept under a treat-and-extend (TAE) regimen were switched to faricimab and studied prospectively. Best-corrected visual acuity (BCVA; in logarithm of the minimum angle of resolution), central retinal thickness (CRT), central choroidal thickness (CCT), and exudative status were analyzed using optical coherence tomography. Aqueous humor was collected before and after the switch, and angiopoietin-2 (Ang-2), placental growth factor (PlGF), and vascular endothelial growth factor (VEGF) A levels were measured. RESULTS. After switching from aflibercept to faricimab, exudative changes improved in 28 eyes (52%), remained stable in eight eyes (15%), and worsened in 18 eyes (33%). BCVA changed from 0.27 ± 0.31 to 0.26 ± 0.29 (P = 0.46), CRT decreased from 306.2 ± 147.5 μm to 278.6 ± 100.4 μm (P = 0.11), and CCT changed from 189.5 ± 92.8 μm to 186.8 ± 93.9 μm (P = 0.21). VEGF-A levels were below the detection sensitivity in many cases throughout the pre- and post-switching periods. Ang-2 significantly decreased from 23.8 ± 23.5 pg/mL to 16.4 ± 21.9 pg/mL (P < 0.001), and PlGF significantly increased from 0.86 ± 0.85 pg/mL to 1.72 ± 1.39 pg/mL (P < 0.001). CONCLUSIONS. Switching from aflibercept to faricimab in patients with nAMD may not only suppress VEGF-A but also Ang-2 and reduce exudative changes.

DOI： 10.1167/iovs.65.11.15

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Visualization of distribution in the vitreous cavity via eye drops using ultra-heavily T2-weighted sequences in MRI: a preliminary study with enucleated pig eyes

Kato, Y; Yuki, K; Nishiguchi, K; Naganawa, S

RADIOLOGICAL PHYSICS AND TECHNOLOGY   17 巻 ( 3 ) 頁： 715 - 724   2024年9月

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記述言語：英語   出版者・発行元：Radiological Physics and Technology  

We investigated whether magnetic resonance imaging can visualize the distribution in the vitreous cavity via eye drops of ophthalmic solutions, gadolinium-based contrast agent, and 17O-water, and to clarify the usefulness of ultra-heavily T2-weighted sequences in the research of intraocular distribution. Five different solutions (V-ROHTO, TRAVATANZ, gadobutrol, H217O, and saline) were administered to excised pig eye specimens. The samples were scanned using T1 mapping, T2 mapping, 3D T2-weighted (echo times (TE): 500, 3200, and 4500 ms), a half-Fourier single-shot turbo-spin echo sequence (HASTE; TE: 440 and 3000 ms), and 3D-real inversion-recovery before eye drops administration. Subsequently, we used a plastic dropper to drop a 0.5 mL solution each, and images were obtained up to 26 h later. Temporal changes in the T1 and T2 values of the anterior chamber and vitreous cavity were compared. The other sequences were evaluated by determining temporal signal changes as signal intensity ratio (SIR) compared to “No drop.” The T1 and T2 values of samples treated with gadobutrol and H217O decreased over time. The SIR of samples treated with gadobutrol and H217O showed remarkable changes in the 3D T2-weighted images, whereas no remarkable temporal changes were observed in the other solutions. Longer TEs resulted in remarkable changes. We demonstrated that visualization of distribution in the vitreous cavity via eye drops could be achieved with excised pig eyes using gadobutrol and H217O, but not with ophthalmic solutions. Ultra-heavily T2-weighted sequences may be promising for the early and highly sensitive visualization of the intraocular distribution of eye drops.

DOI： 10.1007/s12194-024-00826-6

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本邦における杆体一色覚の遺伝的病因と臨床的特徴

井岡 大河, 林 孝彰, 溝渕 圭, 角田 和繁, 國吉 一樹, 近藤 寛之, 太田 淳也, 後藤 健介, 小南 太郎, 西口 康二, 上野 真治

眼科臨床紀要   17 巻 ( 8 ) 頁： 635 - 635   2024年8月

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記述言語：日本語   出版者・発行元：眼科臨床紀要会  

Specification of variant interpretation guidelines for inherited retinal dystrophy in Japan

Fujinami, K; Nishiguchi, KM; Oishi, A; Akiyama, M; Ikeda, Y

JAPANESE JOURNAL OF OPHTHALMOLOGY   68 巻 ( 4 ) 頁： 389 - 399   2024年7月

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記述言語：英語   出版者・発行元：Japanese Journal of Ophthalmology  

Accurate interpretation of sequence variants in inherited retinal dystrophy (IRD) is vital given the significant genetic heterogeneity observed in this disorder. To achieve consistent and accurate diagnoses, establishment of standardized guidelines for variant interpretation is essential. The American College of Medical Genetics and Genomics/Association for Molecular Pathology (ACMG/AMP) guidelines for variant interpretation serve as the global “cross-disease” standard for classifying variants in Mendelian hereditary disorders. These guidelines propose a systematic approach for categorizing variants into 5 classes based on various types of evidence, such as population data, computational data, functional data, and segregation data. However, for clinical genetic diagnosis and to ensure standardized diagnosis and treatment criteria, additional specifications based on features associated with each disorder are necessary. In this context, we present a comprehensive framework outlining the newly specified ACMG/AMP rules tailored explicitly to IRD in the Japanese population on behalf of the Research Group on Rare and Intractable Diseases (Ministry of Health, Labour and Welfare of Japan). These guidelines consider disease frequencies, allele frequencies, and both the phenotypic and the genotypic characteristics unique to IRD in the Japanese population. Adjustments and modifications have been incorporated to reflect the specific requirements of the population. By incorporating these IRD-specific factors and refining the existing ACMG/AMP guidelines, we aim to enhance the accuracy and consistency of variant interpretation in IRD cases, particularly in the Japanese population. These guidelines serve as a valuable resource for ophthalmologists and clinical geneticists involved in the diagnosis and treatment of IRD, providing them with a standardized framework to assess and classify genetic variants.

DOI： 10.1007/s10384-024-01063-5

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特集 第77回日本臨床眼科学会講演集[4] 原著 HLA-A26陽性ベーチェット病との鑑別に苦慮した真菌性眼内炎の1例

新美 渚, 井岡 大河, 鈴村 文那, 牛田 宏昭, 西口 康二

臨床眼科   78 巻 ( 6 ) 頁： 716 - 721   2024年6月

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出版者・発行元：株式会社医学書院  

DOI： 10.11477/mf.1410215196

CiNii Research

Mice with deficiency in <i>Pcdh15</i>, a gene associated with bipolar disorders, exhibit significantly elevated diurnal amplitudes of locomotion and body temperature

Mori, D; Inami, C; Ikeda, R; Sawahata, M; Urata, S; Yamaguchi, ST; Kobayashi, Y; Fujita, K; Arioka, Y; Okumura, H; Kushima, I; Kodama, A; Suzuki, T; Hirao, T; Yoshimi, A; Sobue, A; Ito, T; Noda, Y; Mizoguchi, H; Nagai, T; Kaibuchi, K; Okabe, S; Nishiguchi, K; Kume, K; Yamada, K; Ozaki, N

TRANSLATIONAL PSYCHIATRY   14 巻 ( 1 ) 頁： 216   2024年5月

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記述言語：英語   出版者・発行元：Translational Psychiatry  

Genetic factors significantly affect the pathogenesis of psychiatric disorders. However, the specific pathogenic mechanisms underlying these effects are not fully understood. Recent extensive genomic studies have implicated the protocadherin-related 15 (PCDH15) gene in the onset of psychiatric disorders, such as bipolar disorder (BD). To further investigate the pathogenesis of these psychiatric disorders, we developed a mouse model lacking Pcdh15. Notably, although PCDH15 is primarily identified as the causative gene of Usher syndrome, which presents with visual and auditory impairments, our mice with Pcdh15 homozygous deletion (Pcdh15-null) did not exhibit observable structural abnormalities in either the retina or the inner ear. The Pcdh15-null mice showed very high levels of spontaneous motor activity which was too disturbed to perform standard behavioral testing. However, the Pcdh15 heterozygous deletion mice (Pcdh15-het) exhibited enhanced spontaneous locomotor activity, reduced prepulse inhibition, and diminished cliff avoidance behavior. These observations agreed with the symptoms observed in patients with various psychiatric disorders and several mouse models of psychiatric diseases. Specifically, the hyperactivity may mirror the manic episodes in BD. To obtain a more physiological, long-term quantification of the hyperactive phenotype, we implanted nano tag® sensor chips in the animals, to enable the continuous monitoring of both activity and body temperature. During the light-off period, Pcdh15-null exhibited elevated activity and body temperature compared with wild-type (WT) mice. However, we observed a decreased body temperature during the light-on period. Comprehensive brain activity was visualized using c-Fos mapping, which was assessed during the activity and temperature peak and trough. There was a stark contrast between the distribution of c-Fos expression in Pcdh15-null and WT brains during both the light-on and light-off periods. These results provide valuable insights into the neural basis of the behavioral and thermal characteristics of Pcdh15-deletion mice. Therefore, Pcdh15-deletion mice can be a novel model for BD with mania and other psychiatric disorders, with a strong genetic component that satisfies both construct and surface validity.

DOI： 10.1038/s41398-024-02952-6

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Decrease in electrolyte after vitrectomy surgery may affect the results of forensic investigations using vitreous humor

Ushida, H; Suzumura, A; Yamada, K; Shimizu, H; Suzuki, A; Ishikawa, Y; Kikuchi, R; Nishiguchi, KM; Kaneko, H

BMC OPHTHALMOLOGY   24 巻 ( 1 ) 頁： 219   2024年5月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：BMC Ophthalmology  

Purpose: Vitreous humor (VH) is used for postmortem biochemical studies because it is well protected in an uncontaminated state even after death. The goal of this research was to investigate electrolyte concentrations in the VH from human eyes with and without a history of vitrectomy surgery. Methods: We analyzed the sodium (Na), potassium (K), chloride (Cl) and magnesium (Mg) concentrations from 34 VH samples from 34 patients. Eleven samples were from eyes with a history of vitrectomy, and the remaining 23 eyes had no history of vitrectomy. The correlations of Na, K, Cl and Mg concentrations with patient age, interval between first and second vitrectomy, and lens status (history of cataract surgery) were also evaluated. Results: The Na, K, Cl and Mg concentrations in VH from vitrectomized eyes were 134.1 ± 7.9 mmol/L, 3.7 ± 0.2 mmol/L, 99.7 ± 6.7 mmol/L and 0.59 ± 0.09 mmol/L, respectively; all were significantly lower than the corresponding concentrations in VH from control eyes (lower by 5.0%, 11.0%, 11.7%, and 22.6%, respectively). Na, K, Cl and Mg concentrations in VH from vitrectomized eyes did not show significant correlations with patient ages or the interval between their first and second vitrectomies. There were no significant differences in Na, K, Cl and Mg concentrations in VH between phakic eyes and intraocular lens-implanted eyes. Conclusions: With the increasing number of vitrectomies being performed, it is necessary to consider the history of vitrectomy when using a subject’s VH in forensic examination.

DOI： 10.1186/s12886-024-03445-2

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その他リンク： https://link.springer.com/article/10.1186/s12886-024-03445-2/fulltext.html

Eulerian finite volume method using Lagrangian markers with reference map for incompressible fluid-structure interaction problems

Nishiguchi, K; Shimada, T; Peco, C; Kondo, K; Okazawa, S; Tsubokura, M

COMPUTERS & FLUIDS   274 巻   2024年4月

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出版者・発行元：Computers and Fluids  

We propose a monolithic fluid–structure interaction (FSI) method that presents the advantages of both the reference map technique (RMT) and the Lagrangian Markers approach on a unified, cell-centered finite volume Eulerian framework. Full Eulerian methods that use a Cartesian mesh are attractive for FSI problems that require large-scale computing and involve complex geometries and large solid deformations. However, conventional full Eulerian methods use the velocity gradient to evaluate solid deformations, hence they suffer from numerical instability caused by the discontinuity of the velocity gradient near the interface. In this work, we develop a novel algorithm that interpolates and transfers a reference mapping information field between a collection of Lagrangian Markers and a Eulerian finite volume framework. As a result of integrating these approaches, our method is able to (1) evaluate solid deformations without computing the velocity gradient in the Eulerian framework thanks to RMT, and (2) remove the numerical dissipation of interfaces and internal variables caused by advection in the full Eulerian RMT, thanks to the use of the Lagrangian Markers to compute the constitutive equations. We illustrate with numerical examples that the proposed method preserves geometrical features and yields more accurate results for the deformation and energy than conventional Eulerian FSI method and the full Eulerian RMT.

DOI： 10.1016/j.compfluid.2024.106210

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特集 第77回日本臨床眼科学会講演集[2] 原著 病初期より視神経炎を認めた再発性多発軟骨炎の1例

牛田 知子, 牛田 宏昭, 夏目 啓吾, 鈴村 文那, 西口 康二

臨床眼科   78 巻 ( 4 ) 頁： 505 - 510   2024年4月

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出版者・発行元：株式会社医学書院  

DOI： 10.11477/mf.1410215151

CiNii Research

Detection of elusive DNA copy-number variations in hereditary disease and cancer through the use of noncoding and off-target sequencing reads.

Quinodoz M, Kaminska K, Cancellieri F, Han JH, Peter VG, Celik E, Janeschitz-Kriegl L, Schärer N, Hauenstein D, György B, Calzetti G, Hahaut V, Custódio S, Sousa AC, Wada Y, Murakami Y, Fernández AA, Hernández CR, Minguez P, Ayuso C, Nishiguchi KM, Santos C, Santos LC, Tran VH, Vaclavik V, Scholl HPN, Rivolta C

American journal of human genetics   111 巻 ( 4 ) 頁： 701 - 713   2024年4月

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記述言語：英語   出版者・発行元：American Journal of Human Genetics  

Copy-number variants (CNVs) play a substantial role in the molecular pathogenesis of hereditary disease and cancer, as well as in normal human interindividual variation. However, they are still rather difficult to identify in mainstream sequencing projects, especially involving exome sequencing, because they often occur in DNA regions that are not targeted for analysis. To overcome this problem, we developed OFF-PEAK, a user-friendly CNV detection tool that builds on a denoising approach and the use of “off-target” DNA reads, which are usually discarded by sequencing pipelines. We benchmarked OFF-PEAK on data from targeted sequencing of 96 cancer samples, as well as 130 exomes of individuals with inherited retinal disease from three different populations. For both sets of data, OFF-PEAK demonstrated excellent performance (>95% sensitivity and >80% specificity vs. experimental validation) in detecting CNVs from in silico data alone, indicating its immediate applicability to molecular diagnosis and genetic research.

DOI： 10.1016/j.ajhg.2024.03.001

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INCIDENCE AND RISK FACTORS OF INTRAOCULAR INFLAMMATION AFTER BROLUCIZUMAB TREATMENT IN JAPAN: A Multicenter Age-Related Macular Degeneration Study.

Inoda S, Takahashi H, Maruyama-Inoue M, Ikeda S, Sekiryu T, Itagaki K, Matsumoto H, Mukai R, Nagai Y, Ohnaka M, Kusuhara S, Miki A, Okada AA, Nakayama M, Nishiguchi KM, Takeuchi J, Mori R, Tanaka K, Honda S, Kohno T, Koizumi H, Miyara Y, Inoue Y, Takana H, Iida T, Maruko I, Hayashi A, Ueda-Consolvo T, Yanagi Y

Retina (Philadelphia, Pa.)   44 巻 ( 4 ) 頁： 714 - 722   2024年4月

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記述言語：英語   出版者・発行元：Retina  

Purpose:To investigate the incidence of intraocular inflammation (IOI) and its risk factors following intravitreal injections of brolucizumab for neovascular age-related macular degeneration in Japan.Methods:A total of 1,351 Japanese consecutive patients with neovascular age-related macular degeneration who were treated with brolucizumab from May 2020 to May 2022 at 14 institutions were examined. The variables analyzed were the number of brolucizumab injections, time to onset of IOI, and risk factors.Results:Intraocular inflammation developed in 152 eyes (11.3%). Retinal vasculitis and/or retinal occlusion occurred in 53 eyes (3.9%). Ninety-four patients received bilaterally, bilateral IOI occurred in five patients (5.3%). Sixteen eyes (1.2%) had irreversible visual acuity loss and nine eyes (0.67%) had visual loss of three lines or more due to retinal vasculitis and/or retinal occlusion. The cumulative IOI incidence was 4.5%, 10.3%, and 12.2% at 30, 180, and 365 days (1-year), respectively. History of IOI (including retinal vasculitis) and/or retinal occlusion (odds ratio [OR], 5.41; P = 0.0075) and female sex (OR, 1.99; P = 0.0004) were significantly associated with IOI onset.Conclusion:The 1-year cumulative incidence of IOI in Japanese neovascular age-related macular degeneration patients treated with brolucizumab was 12.2%. History of IOI (including retinal vasculitis) and/or retinal occlusion and female sex were significant risk factors.

DOI： 10.1097/IAE.0000000000004009

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Disease-specific variant interpretation highlighted the genetic findings in 2325 Japanese patients with retinitis pigmentosa and allied diseases

Goto, K; Koyanagi, Y; Akiyama, M; Murakami, Y; Fukushima, M; Fujiwara, K; Iijima, H; Yamaguchi, M; Endo, M; Hashimoto, K; Ishizu, M; Hirakata, T; Mizobuchi, K; Takayama, M; Ota, J; Sajiki, A; Kominami, T; Ushida, H; Fujita, K; Kaneko, H; Ueno, S; Hayashi, T; Terao, C; Hotta, Y; Murakami, A; Kuniyoshi, K; Kusaka, S; Wada, Y; Abe, T; Nakazawa, T; Ikeda, Y; Momozawa, Y; Sonoda, KH; Nishiguchi, KM

JOURNAL OF MEDICAL GENETICS     2024年3月

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記述言語：英語   出版者・発行元：Journal of Medical Genetics  

Background As gene-specific therapy for inherited retinal dystrophy (IRD) advances, unified variant interpretation across institutes is becoming increasingly important. This study aims to update the genetic findings of 86 retinitis pigmentosa (RP)-related genes in a large number of Japanese patients with RP by applying the standardised variant interpretation guidelines for Japanese patients with IRD (J-IRD-VI guidelines) built upon the American College of Medical Genetics and Genomics and the Association for Molecular Pathology rules, and assess the contribution of these genes in RP–allied diseases. Methods We assessed 2325 probands with RP (n=2155, including n=1204 sequenced previously with the same sequencing panel) and allied diseases (n=170, newly analysed), including Usher syndrome, Leber congenital amaurosis and cone-rod dystrophy (CRD). Target sequencing using a panel of 86 genes was performed. The variants were interpreted according to the J-IRD-VI guidelines. Results A total of 3564 variants were detected, of which 524 variants were interpreted as pathogenic or likely pathogenic. Among these 524 variants, 280 (53.4%) had been either undetected or interpreted as variants of unknown significance or benign variants in our earlier study of 1204 patients with RP. This led to a genetic diagnostic rate in 38.6% of patients with RP, with EYS accounting for 46.7% of the genetically solved patients, showing a 9% increase in diagnostic rate from our earlier study. The genetic diagnostic rate for patients with CRD was 28.2%, with RP-related genes significantly contributing over other allied diseases. Conclusion A large-scale genetic analysis using the J-IRD-VI guidelines highlighted the population-specific genetic findings for Japanese patients with IRD; these findings serve as a foundation for the clinical application of gene-specific therapies.

DOI： 10.1136/jmg-2023-109750

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Genetic and Clinical Features of ABCA4-Associated Retinopathy in a Japanese Nationwide Cohort.

Mizobuchi K, Hayashi T, Tanaka K, Kuniyoshi K, Murakami Y, Nakamura N, Torii K, Mizota A, Sakai D, Maeda A, Kominami T, Ueno S, Kusaka S, Nishiguchi KM, Ikeda Y, Kondo M, Tsunoda K, Hotta Y, Nakano T

American journal of ophthalmology   264 巻   頁： 36 - 43   2024年3月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：American Journal of Ophthalmology  

Purpose: To clarify the genetic and clinical features of Japanese patients with ABCA4-associated retinopathy. Design: Retrospective, multicenter cohort study. Methods: Patients with retinal degeneration and biallelic ABCA4 variants were recruited from 13 different hospitals. Whole exome sequencing analysis was used for genetic testing. Comprehensive ophthalmic examinations were performed on matched patients. The primary outcome measure was identifying multimodal retinal imaging findings associated with disease progression. Results: This study included 63 patients: 19 with missense/missense, 23 with missense/truncation, and 21 with truncation/truncation genotypes. In total, 62 variants were identified, including 29 novel variants. Six patients had a mild phenotype characterized by foveal-sparing or preserved foveal structure, including 4 with missense/missense and 2 with missense/truncation genotypes. The p.Arg212His variant was the most frequent in patients with mild phenotypes (4/12 alleles). Clinical findings showed a disease duration-dependent worsening of the phenotypic stage. Patients with the truncation/truncation genotype exhibited rapid retinal degeneration within a few years and definite fundus autofluorescence imaging patterns, including hyper autofluorescence at the macula and few or no flecks. Conclusions: Our results indicate that missense/missense or missense/truncation genotypes, including the p.Arg212His variant, are associated with a relatively mild phenotype. In contrast, the truncation/truncation genotype causes rapid and severe retinal degeneration in Japanese patients with ABCA4-associated retinopathy. These data are vital in predicting patient prognosis, guiding genetic counseling, and stratifying patients for future clinical trials.

DOI： 10.1016/j.ajo.2024.03.007

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Secondary Angle Closure Caused by Anterior Displacement of Capsular Tension Ring and Intraocular Lens Due to Aqueous Misdirection

Goto, K; Tomita, R; Hiraiwa, J; Kawabe, M; Nishiguchi, KM; Yuki, K

CUREUS JOURNAL OF MEDICAL SCIENCE   16 巻 ( 3 ) 頁： e55716   2024年3月

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記述言語：英語  

DOI： 10.7759/cureus.55716

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特集 先端医療を先取りしよう-日本にはない海外の医療 企画にあたって

西口 康二

臨床眼科   78 巻 ( 2 ) 頁： 159 - 159   2024年2月

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出版者・発行元：株式会社医学書院  

DOI： 10.11477/mf.1410215088

CiNii Research

Accuracy of pattern deviation in estimating the glaucomatous damage in the central 10° visual field in eyes with glaucoma and cataract

Tomita, R; Asaoka, R; Hirasawa, K; Fujino, Y; Nakakura, S; Murata, H; Omura, T; Shoji, N; Obana, A; Nishiguchi, KM; Tanito, M

BRITISH JOURNAL OF OPHTHALMOLOGY   108 巻 ( 1 ) 頁： 78 - 83   2024年1月

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記述言語：英語   出版者・発行元：British Journal of Ophthalmology  

Background/aims The accuracy of pattern deviation (PD) in estimating the damage to the glaucomatous visual field (VF) in the central 10° in eyes with glaucoma and cataract is unclear. Methods This retrospective study includes 63 eyes of 52 glaucoma patients who successfully underwent cataract surgery or cataract surgery plus iStent implantation. Using the Humphrey Field Analyser 10-2 test, VF was measured within 6 months preoperatively and postoperatively (VF pre and VF post, respectively). The mean total deviation values in VF post (mTD post) indicates glaucomatous damage without cataract and the difference between this value and mean PD values in VF pre (mPD pre) was evaluated (ϵmPD). The effect of cataract was then evaluated as the difference between mTD post and mTD pre ( "mTD), while the effects of mTD post and "mTD on ϵmPD were also assessed. In addition, based on preoperative visual acuity (VA pre) and VF pre, the optimal model for predicting mTD post was identified. The error of this method (ϵOptimalModel) was estimated as the difference against mTD post, which was compared with ϵmPD. Results Compared with mTD pre, there was a significant improvement in mTD post (p=0.028). A significant difference was observed between mPD pre and mTD post (p<0.001). Further, ϵmPD significantly increased with the increase of mTD post or "mTD (p<0.001 and p=0.0444, respectively). The absolute ϵOptimalModel was significantly smaller than the absolute ϵmPD (p<0.001). Conclusions This study warns clinicians that PD of the central 10° VF might underestimate the glaucomatous VF damage with the progression of glaucoma and overestimate it as a cataract progresses.

DOI： 10.1136/bjo-2022-322274

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Identification of OPTN p.(Asn51Thr): A novel pathogenic variant in primary open-angle glaucoma.

Shiga Y, Hashimoto K, Fujita K, Maekawa S, Sato K, Kubo S, Kawase K, Tokumo K, Kiuchi Y, Mori S, Nakamura M, Iwata T, Nishiguchi KM, Nakazawa T

Genetics in medicine open   2 巻   頁： 100839 - 100839   2024年

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Genetics in Medicine Open  

Purpose: Pathogenic variants in TBK1, MYOC, and OPTN are associated with primary open-angle glaucoma (POAG) with severe visual field defects. This study aims to understand further POAG-related pathogenic variant(s) based on a cohort of East Asian populations that have not been well-characterized. Methods: We conducted a comprehensive screening of TBK1, MYOC, and OPTN variants in 174 POAG Japanese patients, followed by 8380 population-specific genome sequencing data references, segregation analysis, and functional protein assays to determine pathogenic variants. Results: Despite the small sample size, 4 variants were novel, 2 of which p.(Cys5Trp) and p.(Thr293Met) were in the MYOC gene, and 2 p.(Asn51Thr), and p.(Gln142His) were in the OPTN. Notably, the OPTN p.(Asn51Thr) missense variant adjacent to the p.(Glu50Lys) variant, a well-known POAG pathogenic variant, was segregated from all proband's family members with POAG. Moreover, in silico and in vitro analyses revealed that the OPTN p.(Asn51Thr) protein increased binding instability, interactions of the OPTN-TBK1 complex, and enhanced protein insolubility, likewise the p.(Glu50Lys) protein. Conclusion: Our findings may provide further genetic insights into rare variants of POAG and support the clear conclusion that OPTN p.(Asn51Thr) is a novel likely pathogenic variant.

DOI： 10.1016/j.gimo.2023.100839

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網膜遺伝子治療の夜明け～いま何が問題か？～

西口 康二

日本視能訓練士協会誌   54 巻 ( 0 ) 頁： 1 - 6   2024年

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記述言語：日本語   出版者・発行元：公益社団法人 日本視能訓練士協会  

<p>2008年に先天性に発症する重症な網膜ジストロフィに対する遺伝子治療の成功が初めて報告されて15年が経過し、2023年に同治療がルクスターナ^TMとして本邦で薬事承認された。これによって、眼科領域でも本格的にゲノム医療の時代が幕を開けた。しかし、同治療により、高度に低下していた網膜感度が大幅に改善させることが可能となった一方で、低視力の問題は解決しないことや術後に網脈絡膜萎縮が発症することなどの課題が報告されている。また、保険診療で遺伝病に対する遺伝子特異的な治療を行うにあたっては、保険診療のもとで広く遺伝子検査を実施するシステムの整備が必要となる。本稿では、本邦での網膜遺伝子治療に関する諸問題について考察する。</p>

DOI： 10.4263/jorthoptic.54t001

CiNii Research

Disease-specific variant interpretation highlighted the genetic findings in 2325 Japanese patients with retinitis pigmentosa and allied diseases

Kensuke Goto, Yoshito Koyanagi, Masato Akiyama, Yusuke Murakami, Masatoshi Fukushima, Kohta Fujiwara, Hanae Iijima, Mitsuyo Yamaguchi, Mikiko Endo, Kazuki Hashimoto, Masataka Ishizu, Toshiaki Hirakata, Kei Mizobuchi, Masakazu Takayama, Junya Ota, Ai Fujita Sajiki, Taro Kominami, Hiroaki Ushida, Kosuke Fujita, Hiroki Kaneko, Shinji Ueno, Takaaki Hayashi, Chikashi Terao, Yoshihiro Hotta, Akira Murakami, Kazuki Kuniyoshi, Shunji Kusaka, Yuko Wada, Toshiaki Abe, Toru Nakazawa, Yasuhiro Ikeda, Yukihide Momozawa, Koh-Hei Sonoda, Koji M. Nishiguchi

    2023年11月

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出版者・発行元：Cold Spring Harbor Laboratory  

Abstract

Background

As gene-specific therapy for inherited retinal dystrophy (IRD) advances, unified variant interpretation across institutes is becoming increasingly important. This study aims to update the genetic findings of 86 retinitis pigmentosa (RP)–related genes in a large number of Japanese RP patients by applying the standardized variant interpretation guidelines for Japanese IRD patients (J-IRD-VI guidelines) built upon ACMG/AMP rules and assess the contribution of these genes in RP-allied diseases.

Methods

We assessed 2325 probands with RP (n=2155, including n=1204 sequenced previously with the same sequencing panel) and allied diseases (n=170, all newly analyzed), including Usher syndrome, Leber congenital amaurosis, and cone-rod dystrophy (CRD). Target sequencing using a panel of 86 genes was performed. The variants were interpreted according to the J-IRD-VI guidelines.

Results

A total of 3564 variants were detected, of which 524 variants were interpreted as pathogenic or likely pathogenic. Among these 524 variants, 280 (53.4%) had been either undetected or interpreted as variants of unknown significance or benign variants in our earlier study of 1204 RP patients. This led to a genetic diagnostic rate in 38.6% of RP patients, withEYSaccounting for 46.7% of the genetically solved patients, showing a 9% increase in diagnostic rate from our earlier study. The genetic diagnostic rate for CRD patients was 28.2%, with RP-related genes significantly contributing over other allied diseases.

Conclusion

A large-scale genetic analysis using the J-IRD-VI guidelines highlighted the unique genetic findings for Japanese IRD patients; these findings serve as a foundation for the clinical application of gene-specific therapies.

DOI： 10.1101/2023.11.09.23297953

Abilities of circumpapillary retinal nerve fiber layer thickness and vascular density to discriminate stages in primary open-angle glaucoma 国際誌

Yamaguchi, K; Tomita, R; Koyanagi, Y; Kawase, K; Asaoka, R; Terasaki, H; Iwase, T; Nishiguchi, KM

GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY   262 巻 ( 4 ) 頁： 1221 - 1229   2023年11月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Graefe's Archive for Clinical and Experimental Ophthalmology  

Purpose: To clarify the abilities of circumpapillary retinal nerve fiber layer thickness (cpRNFLT) obtained by optical coherence tomography (OCT) and circumpapillary vessel density (cpVD) measured by OCT-angiography to distinguish different stages in primary open-angle glaucoma determined by 24–2 or 30–2 static visual field (VF) testing. Methods: This retrospective study includes 25 healthy normal eyes of 25 subjects and 87 primary open-angle glaucoma eyes of 87 patients. Areas under the receiver operating characteristic curves (AUROC) were evaluated for determining glaucoma stages using cpRNFLT and cpVD. The absolute errors of the estimated mean total deviation (mTD) using optimal models with cpRNFLT and cpVD were also compared. Results: The AUROCs for discriminating glaucomatous eyes from normal eyes was significantly higher for cpRNFLT than the respective AUROCs for cpVD (0.969 [95% CI 0.939 to 0.998] vs. 0.872 [95% CI 0.806 to 0.938], p = 0.006), whereas cpVD had significantly higher AUROC for discriminating severe glaucoma eyes from moderate glaucoma eyes than cpRNFLT (0.771 [95% CI 0.655 to 0.886] vs. 0.578 [95% CI 0.420 to 0.736], p = 0.022). The mean absolute error in estimating mTD using both cpRNFLT and cpVD was significantly less than the error using cpRNFLT alone (4.56 ± 3.76 dB vs. 5.39 ± 4.00 dB, p = 0.027). Conclusion: Our results suggest that cpVD is better for follow-ups after moderate stage. The combination of cpRNFLT and cpVD may improve VF estimation compared to cpRNFLT alone.

DOI： 10.1007/s00417-023-06302-y

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Retinal ferroptosis as a critical mechanism for the induction of retinochoroiditis during ocular toxoplasmosis

Yamada, K; Tazaki, A; Ushio-Watanabe, N; Usui, Y; Takeda, A; Matsunaga, M; Suzumura, A; Shimizu, H; Zheng, H; Ariefta, NR; Yamamoto, M; Hara, H; Goto, H; Sonoda, KH; Nishiguchi, KM; Kato, M; Nishikawa, Y; Toyokuni, S; Kaneko, H

REDOX BIOLOGY   67 巻   頁： 102890   2023年11月

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記述言語：英語   出版者・発行元：Redox Biology  

Toxoplasmosis is a major infectious disease, affecting approximately one-third of the world's population; its main clinical manifestation, ocular toxoplasmosis (OT), is a severe sight-threatening disease. Nevertheless, the diagnosis of OT is based on clinical findings, which needs improvement, even with biochemical tests, such as polymerase chain reaction and antibody detections. Furthermore, the efficacy of OT-targeted treatment is limited; thus, additional measures for diagnosis and treatments are needed. Here, we for the first time report a significantly reduced iron concentration in the vitreous humor (VH) of human patients infected with OT. To obtain further insights into molecular mechanisms, we established a mouse model of T. gondii infection, in which intravitreally injected tracer 57Fe, was accumulated in the neurosensory retina. T. gondii-infected eyes showed increased lipid peroxidation, reduction of glutathione peroxidase-4 expression and mitochondrial deformity in the photoreceptor as cristae loss. These findings strongly suggest the involvement of ferroptotic process in the photoreceptor of OT. In addition, deferiprone, an FDA-approved iron chelator, reduced the iron uptake but also ameliorated toxoplasma-induced retinochoroiditis by reducing retinal inflammation. In conclusion, the iron levels in the VH could serve as diagnostic markers and iron chelators as potential treatments for OT.

DOI： 10.1016/j.redox.2023.102890

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Ornithine aminotransferase(OAT)遺伝子の複合ヘテロ接合性変異による脳回状脈絡網膜萎縮症の男児例

中野 裕太, 安田 小百合, 溝渕 圭, 池田 麻衣子, 濱島 崇, 上野 真治, 西口 康二, 林 孝彰

日本眼科学会雑誌   127 巻 ( 11 ) 頁： 1069 - 1080   2023年11月

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記述言語：日本語   出版者・発行元：(公財)日本眼科学会  

目的:脳回状脈絡網膜萎縮症(GACR)は,本邦ではまれな常染色体潜性遺伝(劣性遺伝)の先天性代謝異常症で,ビタミンB6(VitB6)依存性オルニチンアミノ基転移酵素活性の低下による高オルニチン血症を特徴とし,進行性の脈絡網膜変性を来す.今回,GACR症例を報告するとともに,過去の日本人GACR報告例の遺伝学的解析結果と比較検討した.症例:3歳,男児.外斜視の精査を目的に紹介受診となった.以前より夜盲を呈していたが,明らかな眼底異常はなく経過観察となった.6歳時の眼底検査で両眼の耳側網膜周辺部に特徴的な脳回状の網脈絡膜萎縮がみられた.全視野刺激網膜電図の杆体系・錐体系反応はいずれも消失していた.高オルニチン血症を認め,GACRが疑われたため,VitB6投与を開始したが,血中オルニチン値は低下しなかった.低蛋白質食治療を併用したところ血中オルニチン値は低下した.最終的に必須アミノ酸を補った蛋白質制限とVitB6投与によって血中オルニチン値の低下が維持された.全エクソーム解析を行い,ornithine aminotransferase(OAT)遺伝子に複合ヘテロ接合性変異(p.Arg271Lysとp.Arg426Ter)が検出された.8歳時の眼底検査で網脈絡膜萎縮は明らかに拡大していた.結論:低蛋白質食治療とVitB6投与の併用によって血中オルニチン値の低下が維持されたが,網脈絡膜萎縮は進行性に拡大した.本症例も含め,過去の日本人GACR症例(13例)のOAT遺伝子変異をまとめた結果,p.Arg426Terが高頻度変異(27%,7/26アレル)であることが見出された.(著者抄録)

今月の表紙 中心性漿液性脈絡網膜症のEn face画像

稲垣 恵子, 西口 康二

臨床眼科   77 巻 ( 10 ) 頁： 1234 - 1234   2023年10月

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出版者・発行元：株式会社医学書院  

DOI： 10.11477/mf.1410214923

CiNii Research

Association Between Torque Teno Virus and Systemic Immunodeficiency in Patients With Uveitis With a Suspected Infectious Etiology 査読有り 国際誌

Sajiki, A; Koyanagi, Y; Ushida, H; Kawano, K; Fujita, K; Okuda, D; Kawabe, M; Yamada, K; Suzumura, A; Kachi, S; Kaneko, H; Komatsu, H; Usui, Y; Goto, H; Nishiguchi, KM

AMERICAN JOURNAL OF OPHTHALMOLOGY   254 巻   頁： 80 - 86   2023年10月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：American Journal of Ophthalmology  

PURPOSE: To determine the correlation between the presence of torque teno virus (TTV) in the aqueous humor of patients with uveitis and clinical information, including immunodeficiency history. DESIGN: Multicenter, retrospective, cross-sectional study. METHODS: Fifty-eight patients with uveitis with a suspected infectious etiology and 24 controls with cataract or age-related macular degeneration were included. We used quantitative polymerase chain reaction to test all subjects for TTV and multiplex polymerase chain reaction to test uveitis subjects for common ocular pathogens. When possible, both serum and aqueous humor samples were tested. Ocular TTV positivity was compared with age, sex, and a history of systemic immunodeficiency with logistic analysis. RESULTS: Ocular TTV positivity was found in 23%, 11%, and 0% of patients with herpetic uveitis, nonherpetic uveitis, and controls, respectively. Among patients with herpes infection, positivity for ocular TTV was found in 43%, 8%, 14%, and 50% of patients with cytomegalovirus retinitis, iridocyclitis, acute retinal necrosis, and Epstein–Barr virus–positive uveitis, respectively. Patients with cytomegalovirus retinitis showed a significantly higher rate of ocular TTV infection than controls (P =.008). Serum analysis revealed TTV positivity in 90% of patients with uveitis and in 100% of controls. Age- and gender-adjusted logistic analysis revealed a correlation between ocular TTV positivity and systemic immunodeficiency (P =.01), but no correlations between ocular TTV and age, gender, or viral pathogenic type. CONCLUSIONS: These findings suggest that positivity for ocular TTV was correlated with a clinical history of systemic immunodeficiency.

DOI： 10.1016/j.ajo.2023.06.012

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Pathogenic variants of <i>MFRP</i> and <i>PRSS56</i> genes are major causes of nanophthalmos in Japanese patients

Ota, J; Inooka, T; Okado, S; Maeda, N; Koyanagi, Y; Kominami, T; Nishiguchi, KM; Ueno, S

OPHTHALMIC GENETICS   44 巻 ( 5 ) 頁： 423 - 429   2023年9月

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記述言語：英語   出版者・発行元：Ophthalmic Genetics  

Background: Nanophthalmos (NNO) is a rare condition with significantly shorter axial length than normal. Several genes are known to cause NNO, among them the MFRP and PRSS56 genes have been reported to cause majority of NNOs. The purpose of this study was to determine the genetic basis of Japanese patients with NNO. Materials and Methods: We studied seven patients with NNO. Whole exome sequencing (WES) and Sanger sequencing were performed to determine the variants causing the NNO. We also reviewed the medical charts of the patients to determine the phenotype of these seven patients. Results: WES revealed that four patients from three families carried homozygous frameshift variants of the PRSS56 gene (c.1066dupC). Two novel variants of the MFRP gene were detected in the other two patients: one proband had a homozygous missense variant (c.1486 G>A) and the other had a compound heterozygous variant (c.1486 G>A and c.662_663insT). The axial length of the eight eyes with the PRSS56 variant was 15.69 ± 0.48 mm (mean ± SD) and that for the 4 eyes with the MFRP variant was 15.63 ± 0.69 mm. Three of the six cases with the PRSS56 or MFRP variant had the uveal effusion syndrome. Conclusions: NNOs in Japanese patients are caused by variants of the PRSS56 and MFRP genes as in other ethnic populations. In addition, two new variants of the MFRP gene were found in our cohort. The phenotypes and anomalies in Japanese patients with NNO were similar to those reported for other ethnic populations.

DOI： 10.1080/13816810.2023.2208220

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Predictive factors for outcomes of half-dose photodynamic therapy combined with aflibercept for pachychoroid neovasculopathy

Takeuchi, J; Ota, H; Nakano, Y; Horiguchi, E; Taki, Y; Ito, Y; Terasaki, H; Nishiguchi, KM; Kataoka, K

GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY   261 巻 ( 8 ) 頁： 2235 - 2243   2023年8月

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記述言語：英語   出版者・発行元：Graefe's Archive for Clinical and Experimental Ophthalmology  

Purpose: To assess the effects of half-dose photodynamic therapy (PDT) combined with an intravitreous aflibercept (IVA) injection for pachychoroid neovasculopathy (PNV) and its predictive factors. Methods: Clinical information of 43 patients (43 eyes) with PNV obtained before and 6 months after treatment with half-dose PDT combined with IVA was retrospectively analyzed. Patients were categorized into the sufficient (25 eyes, 58.1%) or insufficient (18 eyes, 41.9%) group based on resolution or persistence/recurrence of subretinal fluid (SRF), respectively, and clinical data were compared. Macular neovascularization (MNV) change was studied in 30 cases with available pre- and post-treatment optical coherence tomography angiography images. Results: The sufficient group included younger patients with better baseline best-corrected visual acuity (BCVA), more treatment-naïve eyes, and smaller MNV lesions at baseline than the insufficient group (all, P < 0.047). Complete SRF resolution was 81.8% in treatment-naïve eyes and only 33.3% in previously treated eyes. MNV expanded after half-dose PDT was combined with IVA regardless of the treatment outcome (P = 0.003). Conclusion: Half-dose PDT combined with IVA was effective for PNV treatment, especially for younger patients with good baseline BCVA, treatment-naïve eyes, and small MNV sizes at baseline. MNV expanded after treatment regardless of the treatment outcomes.

DOI： 10.1007/s00417-023-06030-3

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Genetic characteristics of retinitis pigmentosa and allied diseases in 2063 Japanese patients

Nishiguchi, KM; Koyanagi, Y; Akiyama, M; Murakami, Y; Iijima, H; Endo, M; Ueno, S; Hayashi, T; Hotta, Y; Murakami, A; Wada, Y; Abe, T; Nakazawa, T; Ikeda, Y; Sonoda, KH; Goto, K

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE   64 巻 ( 8 )   2023年6月

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遺伝性網膜ジストロフィにおける遺伝学的検査のガイドライン

池田 康博, 堀田 喜裕, 近藤 寛之, 西口 康二, 前田 亜希子, 藤波 芳, 大石 明生, 三宅 正裕, 秋山 雅人, 厚生労働科学研究費補助金難治性疾患政策研究事業網膜脈絡膜・視神経萎縮症に関する調査研究班遺伝性網膜ジストロフィにおける遺伝学的検査のガイドライン作成ワーキンググループ

日本眼科学会雑誌   127 巻 ( 6 ) 頁： 628 - 632   2023年6月

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記述言語：日本語   出版者・発行元：(公財)日本眼科学会  

Assessment of factors affecting flicker ERGs recorded with RETeval from data obtained from health checkup screening 国際誌

Inooka, T; Kominami, T; Yasuda, S; Koyanagi, Y; Ota, J; Okado, S; Tomita, R; Ito, Y; Iwase, T; Terasaki, H; Nishiguchi, KM; Ueno, S

PLOS ONE   18 巻 ( 4 ) 頁： e0284686   2023年4月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PLoS ONE  

Purpose To determine the factors significantly associated with the amplitudes and implicit times of the flicker electroretinograms (ERGs) recorded with the RETeval system by analyzing the comprehensive data obtained during a health checkup screening. Methods Flicker ERGs were recorded with the RETeval system from 373 individuals who had a normal fundus and optical coherence tomography images. The sex, age, anthropometric, ophthalmologic, and hematologic data were collected from all participants who were 40- to 89- years-of-age. Univariable and multivariable linear mixed effects regression analyses were performed to identify factors that were significantly associated with the implicit times and amplitudes of the RETeval flicker ERGs. Results Univariable linear mixed effects regression analysis showed significant correlations between the implicit times and the best-corrected visual acuity, the age, the axial length, the blood sugar level, and the blood urea nitrogen level. Analyses by multivariable linear mixed effects regression identified that the axial length (β = 0.28), the age (β = 0.24), and the blood sugar level (β = 0.092) were three independent factors that were significantly correlated with the implicit times of the RETeval flicker ERGs. Univariable linear mixed effects regression analysis also showed significant correlations between the amplitudes of the RETeval flicker ERGs and the age, the platelet count, and the creatinine level. Multivariable linear mixed effects regression models identified the age (β = -0.092), the platelet count (β = 0.099), and the creatinine level (β = -0.12) as three independent factors that were significantly correlated with the amplitudes of the RETeval flicker ERGs. However, the smoking habits, body mass index, and the blood pressure were not significantly correlated with either the implicit times or amplitudes of the RETeval flicker ERGs. Conclusions Our results indicate that the age and some ophthalmologic and hematologic findings but not the anthropometric findings were significantly associated with the implicit times and amplitudes of the RETeval flicker ERGs. Thus, clinicians should remember these factors when analyzing the RETeval flicker ERGs.

DOI： 10.1371/journal.pone.0284686

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特集 第76回日本臨床眼科学会講演集[2] 原著 視神経管狭窄を伴う点状軟骨異形成症の1例

早川 史織, 野々部 典枝, 西口 康二

臨床眼科   77 巻 ( 4 ) 頁： 533 - 536   2023年4月

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出版者・発行元：株式会社医学書院  

DOI： 10.11477/mf.1410214775

CiNii Research

Genome-Wide Association Study of Age-Related Macular Degeneration Reveals 2 New Loci Implying Shared Genetic Components with Central Serous Chorioretinopathy

Akiyama, M; Miyake, M; Momozawa, Y; Arakawa, S; Maruyama-Inoue, M; Endo, M; Iwasaki, Y; Ishigaki, K; Matoba, N; Okada, Y; Yasuda, M; Oshima, Y; Yoshida, S; Nakao, SY; Morino, K; Mori, Y; Kido, A; Kato, A; Yasukawa, T; Obata, R; Nagai, Y; Takahashi, K; Fujisawa, K; Miki, A; Nakamura, M; Honda, S; Ushida, H; Yasuma, T; Nishiguchi, KM; Mori, R; Tanaka, K; Wakatsuki, Y; Yamashiro, K; Kadonosono, K; Terao, C; Ishibashi, T; Tsujikawa, A; Sonoda, KH; Kubo, M; Kamatani, Y

OPHTHALMOLOGY   130 巻 ( 4 ) 頁： 361 - 372   2023年4月

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記述言語：英語   出版者・発行元：Ophthalmology  

Purpose: To investigate the genetic architecture of age-related macular degeneration (AMD) in a Japanese population. Design: Genome-wide association study (GWAS). Participants: Three thousand seven hundred seventy-two patients with AMD and 16 770 control participants from the Japanese population were enrolled in the association analyses. Methods: We conducted a meta-analysis of 2 independent GWASs that included a total of 2663 patients with AMD and 9471 control participants using the imputation reference panel for genotype imputation specified for the Japanese population (n = 3541). A replication study was performed using an independent set of 1109 patients with AMD and 7299 control participants. Main Outcome Measures: Associations of genetic variants with AMD. Results: A meta-analysis of the 2 GWASs identified 6 loci significantly associated with AMD (P < 5.0 × 10–8). Of these loci, 4 were known to be associated with AMD (CFH, C2/FB, TNFRSF10A, and ARMS2), and 2 were novel (rs4147157 near WBP1L and rs76228488 near GATA5). The newly identified associations were confirmed in a replication study (P < 0.01). After the meta-analysis of all datasets, we observed strong associations in these loci (P = 1.88 × 10–12 and P = 1.35 × 10–9 for meta-analysis for rs4147157 and rs76228488, respectively). When we looked up the associations in the reported central serous chorioretinopathy (CSC) GWAS conducted in the Japanese population, both loci were associated significantly with CSC (P = 4.86 × 10–3 and P = 4.28 × 10–3 for rs4147157 and rs76228488, respectively). We performed a genetic colocalization analysis for these loci and estimated that the posterior probabilities of shared causal variants between AMD and CSC were 0.39 and 0.60 for WBP1L and GATA5, respectively. Genetic correlation analysis focusing on the epidemiologically suggested clinical risk factors implicated shared polygenic architecture between AMD and smoking cessation (rg [the measure of genetic correlation] = –0.33; P = 0.01; false discovery rate, 0.099). Conclusions: Our findings imply shared genetic components conferring the risk of both AMD and CSC. Financial Disclosure(s): Proprietary or commercial disclosure may be found after the references.

DOI： 10.1016/j.ophtha.2022.10.034

Web of Science

Scopus

PubMed

遺伝性網膜ジストロフィ2459例の次世代シークエンスから得られた遺伝的特徴

後藤 健介, 小柳 俊人, 秋山 雅人, 村上 祐介, 福嶋 正俊, 藤原 康太, 飯島 花枝, 山口 光代, 橋本 和軌, 石津 正崇, 平形 寿彬, 溝渕 圭, 高山 理和, 佐治木 愛, 小南 太郎, 牛田 宏昭, 藤田 幸輔, 兼子 裕規, 上野 真治, 林 孝彰, 寺尾 知可史, 堀田 喜裕, 村上 晶, 和田 裕子, 阿部 俊明, 中澤 徹, 池田 康博, 桃沢 幸秀, 園田 康平, 西口 康二

日本眼科学会雑誌   127 巻 ( 臨増 ) 頁： 199 - 199   2023年3月

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記述言語：日本語   出版者・発行元：(公財)日本眼科学会  

眼科診断・治療のイノベーション ゲノム医療による眼疾患の診断・治療のイノベーション

西口 康二, 藤田 幸輔, 小柳 俊人, 佐治木 愛, 川野 健一, 山田 和久, 川部 満希, 奥田 大志, 鈴村 文那, 清水 英幸, 牛田 宏昭, 兼子 裕規, 小松 紘之, 臼井 嘉彦, 後藤 浩, 中澤 徹, 加地 秀

日本眼科学会雑誌   127 巻 ( 3 ) 頁： 402 - 422   2023年3月

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記述言語：日本語   出版者・発行元：(公財)日本眼科学会  

眼科領域でのゲノム医療のプレゼンスは現時点では限定的である.しかし,来るべきゲノム医療時代を見据え,ゲノム情報を用いた先端的な研究を牽引することは,臨床検体にアクセスのある眼科アカデミアの使命である.我々は,眼感染症のゲノム診断と網膜ジストロフィに対するゲノム編集遺伝子治療のトランスレーショナルリサーチを重点テーマとして定め,それらの開発に取り組んでいる.本稿では,これらの新しいゲノム診療プラットフォームの構築と臨床実装を目指した研究の最新の成果を紹介する.I.Nanopore長鎖シークエンシングの眼感染症診断への応用 近年,感染症の領域では,培養するプロセスを経ることなく,検体中の微生物群のDNAやRNAを直接精製し,網羅的にシークエンシングする「メタゲノム解析」が研究に広く用いられている.我々は,簡便性・迅速性に優れた人工知能搭載の長鎖シークエンサーであるNanoporeを用いた眼感染症診断のための新しいメタゲノム解析プラットフォームの開発に取り組んでいる.Multiplexポリメラーゼ連鎖反応(mPCR)でヘルペス属ウイルスなどが検出され診断のついた症例の前房水・硝子体液を対象にNanoporeを用いてメタゲノム解析を行ったところ,多くの検体で診断どおりの病原体が確認された.しかし,一部の検体では病原体が検出されず,さらに,微生物ゲノムのコンタミネーションがあることも判明した.そこで,これらの問題を克服し,起炎微生物を網羅的に探索することを目的に,mPCR陰性症例と対照を複数例解析し,結果を両群で比較するメタゲノム関連解析を行った.しかし,解析により起炎微生物由来と推測されるゲノム断片は検出できたものの,それはChlamydia Trachomatisなど複数の微生物が共通に持つ配列であることが後に判明した.以上より,現時点では,メタゲノム関連解析で検出されたmPCR陰性ぶどう膜炎に関連するゲノムの正体は絞り切れていない.現在,検出されたゲノム断片の由来である病原体を特定するために,多面的なゲノム解析を実施中である.また,Nanoporeメタゲノム解析では一部のぶどう膜炎症例においてtorque teno virusが検出された.同ウイルスは,これまで免疫抑制状態との関連が報告されているが,眼科領域での報告は未だ少ない.そこで,ぶどう膜炎症例において,定量的PCR(qPCR)を用いて前房水中のtorque teno virusの有無を調べ,全身性の免疫能低下の病歴との関連を解析した.その結果,ベースに全身性の免疫能低下がある患者では同ウイルスの検出率が高いことが判明した.このことは,torque teno virusの眼内感染と眼内の免疫能低下の関連性を示唆している可能性がある.II.網膜疾患に対するゲノム編集遺伝子治療戦略 現在行われている眼疾患に対する遺伝子治療の多くはadeno-associated virus(AAV)ベクターを用いた遺伝子補充療法である.この治療の基本コンセプトは,機能喪失型の変異を持つ病的細胞に正常コピーの全長遺伝子を導入することで,欠損遺伝子の機能を補完することである.しかし,AAV容量の制約により,遺伝子補充療法では小さい病因遺伝子しか治療できない.したがって,この方法で治療できる日本人の網膜色素変性患者は,現状で全体のわずか数%程度である.それに対して,ゲノム編集遺伝子治療ではゲノム局所を標的とするため,治療対象の遺伝子のサイズに依存せず,理論的にはほとんどの遺伝子変異の治療が可能である.しかし,ゲノム編集遺伝子治療の治療効率は低く,そのことが臨床応用を妨げている.我々は,臨床応用を念頭にAAVゲノム編集遺伝子治療の効率改善に取り組み,3つの異なるゲノム編集手法を開発した.最初に,汎用性の高いプラットフォームとして,変異を正常配列に置換可能な単一AAVゲノム編集遺伝子治療プラットフォームを開発し(変異置換ゲノム編集遺伝子治療),その有効性をマウスモデルで証明した.続いて,塩基の挿入や欠失によって生じるフレームシフト変異を対象とし,ずれたフレームを元に戻すことに主眼を置いたシンプルかつ治療効率の高いゲノム編集法(リフレームゲノム編集遺伝子治療)を開発した.この方法を用いて,日本人網膜色素変性患者の約12%が保有するeyes shut homolog(EYS)遺伝子のS1653Kfs変異を有する患者由来細胞株を対象に,その有効性を実証した.最後に,マイクロホモロジー媒介末端結合(MMEJ)とhomology-independent targeted integration(HITI)の異なる2つのゲノム修復法をコンパクトに組み合わせたMMEJ-Hゲノム編集遺伝子治療を新たに考案し,イントロンなどの非翻訳領域を主なターゲットとする治療の開発を進めている.今後も,幅広い眼疾患に対して,ゲノム関連技術の応用にフォーカスを当てた挑戦的なトランスレーショナルリサーチを展開し,眼科アカデミアとして,ゲノム医療の発展に貢献していきたい.(著者抄録)

眼科診断・治療のイノベーション ゲノム医療による眼疾患の診断・治療のイノベーション

西口 康二, 藤田 幸輔, 小柳 俊人, 佐治木 愛, 川野 健一, 山田 和久, 川部 満希, 奥田 大志, 鈴村 文那, 清水 英幸, 牛田 宏昭, 兼子 裕規, 小松 紘之, 臼井 嘉彦, 後藤 浩, 中澤 徹, 加地 秀

日本眼科学会雑誌   127 巻 ( 3 ) 頁： 402 - 422   2023年3月

　詳細を見る

記述言語：日本語   出版者・発行元：(公財)日本眼科学会  

眼科領域でのゲノム医療のプレゼンスは現時点では限定的である.しかし,来るべきゲノム医療時代を見据え,ゲノム情報を用いた先端的な研究を牽引することは,臨床検体にアクセスのある眼科アカデミアの使命である.我々は,眼感染症のゲノム診断と網膜ジストロフィに対するゲノム編集遺伝子治療のトランスレーショナルリサーチを重点テーマとして定め,それらの開発に取り組んでいる.本稿では,これらの新しいゲノム診療プラットフォームの構築と臨床実装を目指した研究の最新の成果を紹介する.I.Nanopore長鎖シークエンシングの眼感染症診断への応用 近年,感染症の領域では,培養するプロセスを経ることなく,検体中の微生物群のDNAやRNAを直接精製し,網羅的にシークエンシングする「メタゲノム解析」が研究に広く用いられている.我々は,簡便性・迅速性に優れた人工知能搭載の長鎖シークエンサーであるNanoporeを用いた眼感染症診断のための新しいメタゲノム解析プラットフォームの開発に取り組んでいる.Multiplexポリメラーゼ連鎖反応(mPCR)でヘルペス属ウイルスなどが検出され診断のついた症例の前房水・硝子体液を対象にNanoporeを用いてメタゲノム解析を行ったところ,多くの検体で診断どおりの病原体が確認された.しかし,一部の検体では病原体が検出されず,さらに,微生物ゲノムのコンタミネーションがあることも判明した.そこで,これらの問題を克服し,起炎微生物を網羅的に探索することを目的に,mPCR陰性症例と対照を複数例解析し,結果を両群で比較するメタゲノム関連解析を行った.しかし,解析により起炎微生物由来と推測されるゲノム断片は検出できたものの,それはChlamydia Trachomatisなど複数の微生物が共通に持つ配列であることが後に判明した.以上より,現時点では,メタゲノム関連解析で検出されたmPCR陰性ぶどう膜炎に関連するゲノムの正体は絞り切れていない.現在,検出されたゲノム断片の由来である病原体を特定するために,多面的なゲノム解析を実施中である.また,Nanoporeメタゲノム解析では一部のぶどう膜炎症例においてtorque teno virusが検出された.同ウイルスは,これまで免疫抑制状態との関連が報告されているが,眼科領域での報告は未だ少ない.そこで,ぶどう膜炎症例において,定量的PCR(qPCR)を用いて前房水中のtorque teno virusの有無を調べ,全身性の免疫能低下の病歴との関連を解析した.その結果,ベースに全身性の免疫能低下がある患者では同ウイルスの検出率が高いことが判明した.このことは,torque teno virusの眼内感染と眼内の免疫能低下の関連性を示唆している可能性がある.II.網膜疾患に対するゲノム編集遺伝子治療戦略 現在行われている眼疾患に対する遺伝子治療の多くはadeno-associated virus(AAV)ベクターを用いた遺伝子補充療法である.この治療の基本コンセプトは,機能喪失型の変異を持つ病的細胞に正常コピーの全長遺伝子を導入することで,欠損遺伝子の機能を補完することである.しかし,AAV容量の制約により,遺伝子補充療法では小さい病因遺伝子しか治療できない.したがって,この方法で治療できる日本人の網膜色素変性患者は,現状で全体のわずか数%程度である.それに対して,ゲノム編集遺伝子治療ではゲノム局所を標的とするため,治療対象の遺伝子のサイズに依存せず,理論的にはほとんどの遺伝子変異の治療が可能である.しかし,ゲノム編集遺伝子治療の治療効率は低く,そのことが臨床応用を妨げている.我々は,臨床応用を念頭にAAVゲノム編集遺伝子治療の効率改善に取り組み,3つの異なるゲノム編集手法を開発した.最初に,汎用性の高いプラットフォームとして,変異を正常配列に置換可能な単一AAVゲノム編集遺伝子治療プラットフォームを開発し(変異置換ゲノム編集遺伝子治療),その有効性をマウスモデルで証明した.続いて,塩基の挿入や欠失によって生じるフレームシフト変異を対象とし,ずれたフレームを元に戻すことに主眼を置いたシンプルかつ治療効率の高いゲノム編集法(リフレームゲノム編集遺伝子治療)を開発した.この方法を用いて,日本人網膜色素変性患者の約12%が保有するeyes shut homolog(EYS)遺伝子のS1653Kfs変異を有する患者由来細胞株を対象に,その有効性を実証した.最後に,マイクロホモロジー媒介末端結合(MMEJ)とhomology-independent targeted integration(HITI)の異なる2つのゲノム修復法をコンパクトに組み合わせたMMEJ-Hゲノム編集遺伝子治療を新たに考案し,イントロンなどの非翻訳領域を主なターゲットとする治療の開発を進めている.今後も,幅広い眼疾患に対して,ゲノム関連技術の応用にフォーカスを当てた挑戦的なトランスレーショナルリサーチを展開し,眼科アカデミアとして,ゲノム医療の発展に貢献していきたい.(著者抄録)

LONG-TERM MORPHOLOGIC CHANGES IN MACULAR NEOVASCULARIZATION UNDER AFLIBERCEPT TREATMENT WITH A TREAT-AND-EXTEND REGIMEN

Nakano, Y; Takeuchi, J; Horiguchi, E; Ota, H; Taki, Y; Ito, Y; Terasaki, H; Nishiguchi, KM; Kataoka, K

RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES   43 巻 ( 3 ) 頁： 412 - 419   2023年3月

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記述言語：英語   出版者・発行元：Retina  

Purpose:To examine the morphologic changes in macular neovascularization (MNV) secondary to age-related macular degeneration after 2 years of aflibercept treatment under a treat-and-extend (T&E) regimen.Methods:This retrospective study analyzed the medical records for 26 eyes of 25 patients diagnosed with treatment-naive neovascular age-related macular degeneration and treated with aflibercept under a treat-and-extend regimen for 2 years. The areas of the MNV and vascular structures were assessed using swept-source optical coherence tomography angiography at baseline and after 2 years of treatment.Results:The mean MNV area increased significantly from 0.65 ± 0.42 mm2 at baseline to 0.78 ± 0.45 mm2 at 2 years. At 2 years, the mean change in the MNV area from baseline was 22% (interquartile range: 4%-60%). The baseline MNV area was negatively correlated with the change ratio of the MNV areas at 2 years and baseline (R = -0.68, P < 0.001). Nine of the 26 eyes (34.6%) showed newly formed mature vessels, and 7 eyes (26.9%) showed prominently developing preexisting mature vessels.Conclusion:Macular neovascularization expanded and showed vascular maturation under aflibercept treatment with a treat-and-extend regimen. The smaller the MNV at baseline, the greater is its expansion in 2 years.

DOI： 10.1097/IAE.0000000000003676

Web of Science

Scopus

PubMed

Cost-effective sequence analysis of 113 genes in 1,192 probands with retinitis pigmentosa and Leber congenital amaurosis

Panneman, DM; Hitti-Malin, RJ; Holtes, LK; de Bruijn, SE; Reurink, J; Boonen, EGM; Khan, MI; Ali, M; Andréasson, S; De Baere, E; Banfi, S; Bauwens, M; Ben-Yosef, T; Bocquet, B; De Bruyne, M; de la Cerda, B; Coppieters, F; Farinelli, P; Guignard, T; Inglehearn, CF; Karali, M; Kjellström, U; Koenekoop, R; de Koning, B; Leroy, BP; McKibbin, M; Meunier, I; Nikopoulos, K; Nishiguchi, KM; Poulter, JA; Rivolta, C; de la Rua, ER; Saunders, P; Simonelli, F; Tatour, Y; Testa, F; Thiadens, AAHJ; Toomes, C; Tracewska, AM; Tran, HV; Ushida, H; Vaclavik, V; Verhoeven, VJM; van de Vorst, M; Gilissen, C; Hoischen, A; Cremers, FPM; Roosing, S

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY   11 巻   頁： 1112270   2023年2月

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記述言語：英語   出版者・発行元：Frontiers in Cell and Developmental Biology  

Introduction: Retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA) are two groups of inherited retinal diseases (IRDs) where the rod photoreceptors degenerate followed by the cone photoreceptors of the retina. A genetic diagnosis for IRDs is challenging since >280 genes are associated with these conditions. While whole exome sequencing (WES) is commonly used by diagnostic facilities, the costs and required infrastructure prevent its global applicability. Previous studies have shown the cost-effectiveness of sequence analysis using single molecule Molecular Inversion Probes (smMIPs) in a cohort of patients diagnosed with Stargardt disease and other maculopathies. Methods: Here, we introduce a smMIPs panel that targets the exons and splice sites of all currently known genes associated with RP and LCA, the entire RPE65 gene, known causative deep-intronic variants leading to pseudo-exons, and part of the RP17 region associated with autosomal dominant RP, by using a total of 16,812 smMIPs. The RP-LCA smMIPs panel was used to screen 1,192 probands from an international cohort of predominantly RP and LCA cases. Results and discussion: After genetic analysis, a diagnostic yield of 56% was obtained which is on par with results from WES analysis. The effectiveness and the reduced costs compared to WES renders the RP-LCA smMIPs panel a competitive approach to provide IRD patients with a genetic diagnosis, especially in countries with restricted access to genetic testing.

DOI： 10.3389/fcell.2023.1112270

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PubMed

特集 日本の眼の難病-何がどこまでわかってきたのか? 網膜色素変性

小南 太郎, 西口 康二

臨床眼科   77 巻 ( 1 ) 頁： 27 - 36   2023年1月

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出版者・発行元：株式会社医学書院  

DOI： 10.11477/mf.1410214679

CiNii Research

Dimethyl Fumarate Protects Retinal Pigment Epithelium from Blue Light-Induced Oxidative Damage via the Nrf2 Pathway 査読有り

Shimizu, H; Takayama, K; Yamada, K; Suzumura, A; Sato, T; Nishio, Y; Ito, M; Ushida, H; Nishiguchi, KM; Takeuchi, M; Kaneko, H

ANTIOXIDANTS   12 巻 ( 1 )   2023年1月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Antioxidants  

The purpose of this study is to investigate the protective effect of dimethyl fumarate (DMF), the methyl-ester of fumaric acid, against blue-light (BL) exposure in retinal pigment epithelial (RPE) cells. ARPE-19 cells, a human RPE cell line, were cultured with DMF followed by exposure to BL. Reactive oxygen species (ROS) generation, cell viability, and cell death rate were determined. Real-time polymerase chain reaction and Western blotting were performed to determine the change in nuclear factor (erythroid-derived)-like 2 (NRF2) expression. Twenty-seven inflammatory cytokines in the supernatant of culture medium were measured. BL exposure induced ROS generation in ARPE-19 cells, which DMF alleviated in a concentration-dependent manner. BL exposure increased the ARPE-19 cell death rate, which DMF alleviated. BL exposure induced ARPE-19 cell apoptosis, again alleviated by DMF. Under BL exposure, DMF increased the NRF2 mRNA level and promoted NRF2 expression in the nucleus. BL also strongly increased interleukin (IL)-1β and fibroblast growth factor (FGF) expression. BL strongly induced RPE cell damage with apoptotic change while DMF mainly reduced inflammation in BL-induced RPE damage, resulting in blockade of cell death. DMF has a protective effect in RPE cells against BL exposure via activation of the NRF2 pathway.

DOI： 10.3390/antiox12010045

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PubMed

EFFECTS OF HALF-DOSE PHOTODYNAMIC THERAPY ON CHRONIC CENTRAL SEROUS CHORIORETINOPATHY WITH OR WITHOUT MACULAR NEOVASCULARIZATION ASSESSED USING OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY

Nakamura, K; Takeuchi, J; Kataoka, K; Ota, H; Asai, K; Nakano, Y; Horiguchi, E; Taki, Y; Ito, Y; Terasaki, H; Nishiguchi, KM

RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES   42 巻 ( 12 ) 頁： 2346 - 2353   2022年12月

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記述言語：英語   出版者・発行元：Retina  

Purpose:To assess the effects of half-dose photodynamic therapy on subretinal fluid and macular neovascularization (MNV) using optical coherence tomography angiography in patients with chronic central serous chorioretinopathy.Methods:Clinical information on 168 patients (168 eyes) with chronic central serous chorioretinopathy obtained before and 6 months after treatment with half-dose photodynamic therapy was retrospectively analyzed. Patients were categorized into a success (145 eyes) or failure (23 eyes) group based on the absence or presence of subretinal fluid, respectively, and clinical data were compared between them. Macular neovascularization was studied in 147 cases with available optical coherence tomography angiography images. P < 0.05 indicated statistical significance.Results:The success group showed a younger patient age, better posttreatment best-corrected visual acuity, and thicker pretreatment central choroidal thickness (all, P < 0.047) than did the failure group. Regarding MNV analysis, nine, eight, and 130 eyes had definite, possible, and no MNV, respectively, at baseline; among them, 100.0%, 75.0%, and 2.3%, respectively, had MNV at 6 months posttreatment. Patients with definite MNV at baseline were less likely to show successful subretinal fluid resolution.Conclusion:Although half-dose photodynamic therapy is generally effective for the treatment of chronic central serous chorioretinopathy, coexisting MNV may compromise the outcome; thus, optical coherence tomography angiography-based assessment of chronic central serous chorioretinopathy is important.

DOI： 10.1097/IAE.0000000000003604

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Likely pathogenic structural variants in genetically unsolved patients with retinitis pigmentosa revealed by long-read sequencing 国際誌

Sano, Y; Koyanagi, Y; Wong, JH; Murakami, Y; Fujiwara, K; Endo, M; Aoi, T; Hashimoto, K; Nakazawa, T; Wada, Y; Ueno, S; Gao, D; Murakami, A; Hotta, Y; Ikeda, Y; Nishiguchi, KM; Momozawa, Y; Sonoda, KH; Akiyama, M; Fujimoto, A

JOURNAL OF MEDICAL GENETICS   59 巻 ( 11 ) 頁： 1133 - 1138   2022年11月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Journal of Medical Genetics  

Despite the successful identification of causative genes and genetic variants of retinitis pigmentosa (RP), many patients have not been molecularly diagnosed. Our recent study using targeted short-read sequencing showed that the proportion of carriers of pathogenic variants in EYS, the cause of autosomal recessive RP, was unexpectedly high in Japanese patients with unsolved RP. This result suggested that causative genetic variants, which are difficult to detect by short-read sequencing, exist in such patients. Using long-read sequencing technology (Oxford Nanopore), we analysed the whole genomes of 15 patients with RP with one heterozygous pathogenic variant in EYS detected in our previous study along with structural variants (SVs) in EYS and another 88 RP-associated genes. Two large exon-overlapping deletions involving six exons were identified in EYS in two patients with unsolved RP. An analysis of an independent patient set (n=1189) suggested that these two deletions are not founder mutations. Our results suggest that searching for SVs by long-read sequencing in genetically unsolved cases benefits the molecular diagnosis of RP.

DOI： 10.1136/jmedgenet-2022-108428

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ASSESSMENTS OF MACULAR FUNCTION BY FOCAL MACULAR ELECTRORETINOGRAPHY AND STATIC PERIMETRY IN EYES WITH RETINITIS PIGMENTOSA

Okado, S; Koyanagi, Y; Inooka, T; Kominami, T; Terasaki, H; Nishiguchi, KM; Ueno, S

RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES   42 巻 ( 11 ) 頁： 2184 - 2193   2022年11月

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記述言語：英語   出版者・発行元：Retina  

Purpose:To assess the macular function by focal macular electroretinography and static perimetry in eyes with retinitis pigmentosa.Methods:Eighty-eight eyes of 88 retinitis pigmentosa patients were analyzed. The relationships between the focal macular electroretinography components and the mean deviations (MDs) of the Humphrey Field Analyzer 10-2 were determined. Spectral-domain optical coherence tomography was used to determine the integrity of the ellipsoid zone (EZ) and the interdigitation zone.Results:Forward-backward stepwise regression analyses showed that the amplitudes (r = 0.45, P < 0.01) and implicit times (r = -0.29, P < 0.01) of the b-waves were significantly correlated with the MDs. Some of the eyes had reduced b-wave amplitudes (<1.0 µV) and disrupted interdigitation zone, despite having a better MD (≥ -10.0 dB) and intact EZ. Subgroup analyses of eyes with better MD (≥ -10.0 dB) showed that the EZ width was correlated with the MDs but not with the b-wave amplitude. The thickness of the EZ-retinal pigment epithelium as an alternative indicator of interdigitation zone was correlated with the b-wave amplitude (r = 0.32, P = 0.04) but not with the MDs (r = -0.10, P = 0.53).Conclusion:The fact that the focal macular electroretinography amplitudes are reduced before the shortening of the EZ in the early stage of retinitis pigmentosa indicates that the focal macular electroretinography amplitudes are an earlier indicator of macular dysfunction than the Humphrey Field Analyzer 10-2 findings.

DOI： 10.1097/IAE.0000000000003589

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Choroidal hemodynamics in central serous chorioretinopathy after half-dose photodynamic therapy and the effects of smoking 国際誌

Horiguchi, E; Takeuchi, J; Tomita, R; Asai, K; Nakano, Y; Ota, H; Taki, Y; Ito, Y; Terasaki, H; Nishiguchi, KM; Kataoka, K

SCIENTIFIC REPORTS   12 巻 ( 1 ) 頁： 17032 - 17032   2022年10月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Scientific Reports  

This retrospective study aimed to evaluate choroidal hemodynamics after half-dose photodynamic therapy (PDT) for central serous chorioretinopathy (CSC) and the effects of smoking using laser speckle flowgraphy. This study included 29 eyes of 29 patients treated with half-dose PDT for CSC, who were followed-up for at least 6 months. The mean blur rate (MBR) in the PDT irradiation area (whole area), the pachyvessel (PV) area, non-PV (NPV) area, and filling delay (FD) area were assessed at baseline and 1, 3, and 6 months post-PDT, respectively. The MBR was also assessed by smoking status. The MBR significantly decreased from baseline in the whole, PV, NPV, and FD areas at all time points (P < 0.001). Of the 29 patients, 6 were never smokers, 13 were past smokers, and 10 were current smokers. At baseline, no significant difference was found in the MBR in the whole, PV, NPV, and FD areas among never, past, and current smokers. The MBR changes showed a significantly smaller decrease in current smokers than in never smokers in the whole (P = 0.021), PV (P = 0.009), and NPV (P = 0.034) areas, but not in the FD area (P = 0.172). Half-dose PDT for CSC reduced choroidal blood flow in the PDT-irradiated area, which was blunted by current smoking status.

DOI： 10.1038/s41598-022-21584-8

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今月の表紙 サルコイドーシス

平田 泰介, 山本 素士, 西口 康二

臨床眼科   76 巻 ( 9 ) 頁： 1191 - 1191   2022年9月

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出版者・発行元：株式会社医学書院  

DOI： 10.11477/mf.1410214491

CiNii Research

第1土曜特集 五感を科学する--感覚器研究の最前線 視覚 難治性眼疾患に対するin vivo遺伝子治療の最前線

西口 康二

医学のあゆみ   282 巻 ( 6 ) 頁： 634 - 640   2022年8月

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出版者・発行元：医歯薬出版  

DOI： 10.32118/ayu28206634

CiNii Research

綜説 網膜ジストロフィに対する遺伝子治療の進歩

西口 康二

眼科   64 巻 ( 7 ) 頁： 647 - 652   2022年7月

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出版者・発行元：金原出版  

DOI： 10.18888/ga.0000002697

CiNii Research

今月の表紙 アトピー性白内障

坂本 正明, 髙橋 次郎, 西口 康二

臨床眼科   76 巻 ( 6 ) 頁： 722 - 722   2022年6月

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出版者・発行元：株式会社医学書院  

DOI： 10.11477/mf.1410214400

CiNii Research

Switching from aflibercept to brolucizumab for the treatment of refractory neovascular age-related macular degeneration

Ota, H; Takeuchi, J; Nakano, Y; Horiguchi, E; Taki, Y; Ito, Y; Terasaki, H; Nishiguchi, KM; Kataoka, K

JAPANESE JOURNAL OF OPHTHALMOLOGY   66 巻 ( 3 ) 頁： 278 - 284   2022年5月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japanese Journal of Ophthalmology  

Purpose: To examine the 16-week outcomes of switching to brolucizumab in eyes with neovascular age-related macular degeneration (nAMD) refractory to aflibercept. Study design: Retrospective observational study. Methods: Data of eyes with nAMD who switched to brolucizumab because of resistance to aflibercept were collected. The best-corrected visual acuity (BCVA; in logarithm of the minimum angle of resolution), central retinal thickness (CRT), central choroidal thickness (CCT), and exudative status on optical coherence tomography were analyzed. Results: A total of 48 eyes of 48 patients were reviewed. At 4 to 7 weeks after switching, BCVA changed from 0.26 ± 0.19 to 0.25 ± 0.21 (not significant; P = 0.95), but CRT significantly decreased from 298.9 ± 108.4 µm to 241.9 ± 92.5 µm (P < 0.001) and CCT from 182.6 ± 89.3 µm to 169.7 ± 82.6 µm (P < 0.001). Of the 23 eyes refractory to monthly aflibercept injections, 12 (52.2%) achieved a dry macula, and 8 (34.8%) reduced exudative changes at 1 month. At 16 weeks, 31 eyes (64.6%) achieved the treatment interval ≥ 8 weeks. Two patients (4.2%) dropped out, 7 eyes (14.6%) developed intraocular inflammation (IOI), and 8 eyes (16.7%) switched back to aflibercept because of the failure to extend the treatment interval ≥ 8 weeks. Conclusion: Switching to brolucizumab in eyes refractory to aflibercept conferred favorable outcomes in controlling exudative changes. However, IOI and the regulation of the treatment interval to at least 8 weeks during the maintenance phase disrupted the continuation of brolucizumab treatment.

DOI： 10.1007/s10384-022-00908-1

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Ablation of <i>Ctrp9</i>, Ligand of AdipoR1, and Lower Number of Cone Photoreceptors in Mouse Retina 国際誌

Inooka, D; Omori, Y; Ouchi, N; Ohashi, K; Kawakami, Y; Koyanagi, Y; Koike, C; Terasaki, H; Nishiguchi, KM; Ueno, S

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE   63 巻 ( 5 ) 頁： 14 - 14   2022年5月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Investigative Ophthalmology and Visual Science  

PURPOSE. C1q/TNF-related protein (CTRP) 9 is one of the adiponectin paralogs, and a genetic ablation of its receptor, AdipoR1, is known to cause retinal degeneration. The purpose of this study was to determine the role played by CTRP9 in the retina. METHODS. The retinas of Ctrp9 gene knockout (KO) and wild type (WT) mice were examined by electroretinography (ERG), histology, RNA sequencing, and quantitative real-time PCR. RESULTS. The amplitude of the photopic ERG elicited by the maximum stimulus intensity was smaller by 40% in the Ctrp9 KO mice than in WT mice at 8 weeks of age. However, the photopic ERGs was not reduced from 8 weeks to 6 months of age. The amplitudes of the scotopic ERGs were not reduced in the Ctrp9 KO mice at 8 weeks and 6 months of age. No distinct histological abnormalities were found in the retinal sections but the density of peanut agglutinin-stained cells in the retinal flat mount of KO mice was reduced to about 70% of that of WT mice. Genomewide RNA sequencing of the retina revealed the absence of the expression of CTRP9 in both KO and WT mice. RNA sequencing and quantitative real-time PCR analysis showed that the expressions of the transcripts of genes expressed in cones, Opn1sw, Opn1mw, Gnat2, and Cnga3, were reduced in the KO mice retina, however, the degree of expression of the transcripts in rods was not significantly reduced. CONCLUSIONS. CTRP9 is released ectopically from other tissues, and it regulates the number of cones in the mouse retinas.

DOI： 10.1167/iovs.63.5.14

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Clinical findings in eyes with BEST1-related retinopathy complicated by choroidal neovascularization 国際誌

Miyagi, M; Takeuchi, J; Koyanagi, Y; Mizobuchi, K; Hayashi, T; Ito, Y; Terasaki, H; Nishiguchi, KM; Ueno, S

GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY   260 巻 ( 4 ) 頁： 1125 - 1137   2022年4月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Graefe's Archive for Clinical and Experimental Ophthalmology  

Purpose: To determine the characteristics of eyes diagnosed with Best vitelliform macular dystrophy (BVMD) and autosomal recessive bestrophinopathy (ARB) complicated by choroidal neovascularization (CNV). Methods: This was a retrospective, multicenter observational case series. Fourteen genetically confirmed BVMD patients and 9 ARB patients who had been examined in 2 ophthalmological institutions in Japan were studied. The findings in a series of ophthalmic examinations including B-scan optical coherence tomography (OCT) and OCT angiography (OCTA) were reviewed. Results: CNV was identified in 5 eyes (17.9%) of BVMD patients and in 2 eyes (11.1%) of ARB patients. Three of 5 eyes with BVMD were classified as being at the vitelliruptive stage and 2 eyes at the atrophic stage. The CNV in 2 BVMD eyes were diagnosed as exudative because of acute visual acuity reduction, retinal hemorrhage, and intraretinal fluid, while the CNV in 3 BVMD eyes and 2 ARB eyes were diagnosed as non-exudative. The visual acuity of the two eyes with exudative CNV did not improve despite anti-VEGF treatments. None of the eyes with non-exudative CNV had a reduction of their visual acuity for at least 4 years. All of the CNV were located within hyperreflective materials which were detected in 16 eyes (57.1%) of the BVMD eyes and in 7 eyes (38.9%) of the ARB eyes. Conclusions: CNV is a relatively common complication in BEST1-related retinopathy in Asian population as well. The prognosis of eyes with exudative CNV is not always good, and OCTA can detect CNV in eyes possessing hyperreflective materials.

DOI： 10.1007/s00417-021-05447-y

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網膜色素変性の疾患レジストリを用いた病因遺伝子毎の臨床像

池田 康博, 山本 修一, 村上 晶, 川崎 良, 西口 康二, 平見 恭彦, 大石 明生, 坂本 泰二, 日本網膜色素変性レジストリプロジェクトJRPRP

日本眼科学会雑誌   126 巻 ( 臨増 ) 頁： 204 - 204   2022年3月

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記述言語：日本語   出版者・発行元：(公財)日本眼科学会  

網膜色素変性の疾患レジストリを用いた病因遺伝子毎の臨床像

池田 康博, 山本 修一, 村上 晶, 川崎 良, 西口 康二, 平見 恭彦, 大石 明生, 坂本 泰二, 日本網膜色素変性レジストリプロジェクトJRPRP

日本眼科学会雑誌   126 巻 ( 臨増 ) 頁： 204 - 204   2022年3月

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記述言語：日本語   出版者・発行元：(公財)日本眼科学会  

日本人enhanced S-cone syndromeの臨床的および遺伝学的特徴

林 孝彰, 溝渕 圭, 亀谷 修平, 國吉 一樹, 日下 俊次, 上野 真治, 西口 康二, 三宅 養三, 中野 匡

日本眼科学会雑誌   126 巻 ( 1 ) 頁： 7 - 18   2022年1月

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記述言語：日本語   出版者・発行元：(公財)日本眼科学会  

目的:Enhanced S-conesyndrome(ESCS)は網膜電図(ERG)でshort-wavelength-sensitive(S)錐体応答が増大する遺伝性網膜疾患であるが,網膜機能の進行性についてははっきりしていない.日本人ESCS症例に関して,ERGを含む長期臨床経過および遺伝学的特徴について後ろ向きに検討する.対象と方法:ESCSと診断された9例を対象とし,このうち8例の初期臨床像は過去に報告されている.NR2E3遺伝子変異はSanger法で決定された.視力,光干渉断層計を用いた中心窩網膜厚,ERG所見について検討した.結果:初発症状を聴取できた7例は,就学前に夜盲を自覚していた.各施設の初診時平均年齢は36.2歳,平均観察期間は15.0年であった.初診時に視力不良であったほうの眼の視力は経過中に大きく変化していなかった.最終測定時の平均中心窩網膜厚は初回測定時に比べ有意に減少していた.特徴的全視野ERGおよびS錐体ERG所見は全例で観察された.20年以上の長期経過を追えた2例の錐体応答は明らかに減弱していた.全例でNR2E3遺伝子に両アレル変異が検出され,p.Arg104Gln変異が28%(5/18アレル)と最多であった.結論:ERGの結果から,ESCSは進行性の網膜変性疾患であることが確認できた.視力は黄斑部網膜分離を合併しなければ比較的長期にわたり維持される可能性がある.p.Arg104Gln変異が日本人ESCSの主要変異であると考えられた.(著者抄録)

BEST1遺伝子関連網膜症に伴う脈絡膜新生血管に関する臨床所見

宮城 麻衣, 上野 真治, 武内 潤, 小柳 俊人, 小南 太郎, 溝渕 圭, 林 孝彰, 伊藤 逸毅, 寺崎 浩子, 西口 康二

眼科臨床紀要   15 巻 ( 1 ) 頁： 70 - 70   2022年1月

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記述言語：日本語   出版者・発行元：眼科臨床紀要会  

Surgical outcome and pathological findings in macular epiretinal membrane caused by neurofibromatosis type 2. 国際誌

Hiroshi Kunikata, Koji M Nishiguchi, Mika Watanabe, Toru Nakazawa

Digital journal of ophthalmology : DJO   28 巻 ( 1 ) 頁： 12 - 16   2022年

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記述言語：英語  

We present surgical outcomes in a 10-year-old Japanese girl with neurofibromatosis type 2 (NF2)-induced epiretinal membrane (ERM). Her right eye underwent lens-sparing 27-gauge microincision vitrectomy surgery (MIVS) with ERM peeling. Decimal best-corrected visual acuity increased from 0.3 to 0.4 postoperatively. However, abnormal thickening of the macula persisted for 3 years. Staining of the extracted ERM revealed many cells positive for glial fibrillary acidic protein and nestin. Although removal of NF2-induced ERM with MIVS can improve visual acuity, the potential surgical risks require careful consideration on a case-by-case basis.

DOI： 10.5693/djo.02.2021.06.001

PubMed

Seasonal variation in submacular hemorrhages in retinal macroaneurysms and its disappearance in age-related macular degeneration 国際誌

Kaneko, H; Takashi, N; Matsunaga, M; Ito, Y; Takeuchi, J; Terasaki, H; Yatsuya, H; Nishiguchi, KM

GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY   259 巻 ( 12 ) 頁： 3589 - 3596   2021年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Graefe's Archive for Clinical and Experimental Ophthalmology  

Purpose: To investigate whether previously reported seasonal variation and winter-dominant prevalence of acute massive submacular hemorrhages (SMHs) caused by age-related macular degeneration (AMD) disappeared, and those caused by retinal microaneurysms (RMAs) emerged. Method: The medical charts of 95 patients (95 eyes) with SMH caused by AMD and 76 patients (76 eyes) with SMH caused by RMAs in 2012–2019 were retrospectively reviewed. For each subject, the month of onset, the mean ambient temperature of that month were recorded. Results: The monthly numbers of cases of SMHs caused by AMD from January to December were 6, 8, 4, 9, 7, 10, 9, 11, 7, 11, 3, and 10. No significant seasonal variation in the monthly incidence was identified (Roger’s R = 1.89, p = 0.39). The monthly numbers of SMHs caused by RMAs from January to December were 3, 11, 11, 8, 7, 8, 5, 5, 2, 4, 7, and 5. There was significant seasonal variation in the monthly incidence (Roger’s R = 7.67, p = 0.02). There was no significant correlation between the monthly incidence of SMHs caused by RMAs and mean ambient temperature. Conclusion: Our previous study conducted for cases obtained in 1998–2005 showed seasonal cyclic trend in the number of SMHs caused by AMD, with the peak in winter. However, that significant seasonal variation disappeared in 2012–2019 in the present study. Common usage of OCT devices and anti-VEGF drugs might be the reason for the lack of seasonal variation in the cases of SMH caused by AMD. [Figure not available: see fulltext.]

DOI： 10.1007/s00417-021-05280-3

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CHOP deletion and anti-neuroinflammation treatment with hesperidin synergistically attenuate NMDA retinal injury in mice 国際誌

Sato, K; Sato, T; Ohno-Oishi, M; Ozawa, M; Maekawa, S; Shiga, Y; Yabana, T; Yasuda, M; Himori, N; Omodaka, K; Fujita, K; Nishiguchi, KM; Ge, S; Nakazawa, T

EXPERIMENTAL EYE RESEARCH   213 巻   頁： 108826 - 108826   2021年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Experimental Eye Research  

Glaucoma is a leading cause of blindness worldwide and is characterized by degeneration associated with the death of retinal ganglion cells (RGCs). It is believed that glaucoma is a group of heterogeneous diseases with multifactorial pathomechanisms. Here, we investigate whether anti-inflammation treatment with an ER stress blockade can selectively promote neuroprotection against NMDA injury in the RGCs. Retinal excitotoxicity was induced with an intravitreal NMDA injection. Microglial activation and neuroinflammation were evaluated with Iba1 immunostaining and cytokine gene expression. A stable HT22 cell line transfected with an NF-kB reporter was used to assess NF-kB activity after hesperidin treatment. CHOP-deficient mice were used as a model of ER stress blockade. Retinal cell death was evaluated with a TUNEL assay. As results, in the NMDA injury group, Iba1-positive microglia increased 6 h after NMDA injection. Also at 6 h, pro-inflammatory cytokines and chemokine increased, including TNFα, IL-1b, IL-6 and MCP-1. In addition, the MCP-1 promoter-driven EGFP signal, which we previously identified as a stress signal in injured RGCs, also increased; hesperidin treatment suppressed this inflammatory response and reduced stressed RGCs. In CHOP-deficient mice that received an NMDA injection, the gene expression of pro-inflammatory cytokines, chemokines, markers of active microglia, and inflammatory regulators was greater than in WT mice. In WT mice, hesperidin treatment partially prevented retinal cell death after NMDA injury; this neuroprotective effect was enhanced in CHOP-deficient mice. These findings demonstrate that ER stress blockade is not enough by itself to prevent RGC loss due to neuroinflammation in the retina, but it has a synergistic neuroprotective effect after NMDA injury when combined with an anti-inflammatory treatment based on hesperidin.

DOI： 10.1016/j.exer.2021.108826

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特集 網膜色素変性のアップデート 【網膜色素変性:最近の話題】 SAG遺伝子変異スペクトラムとしての小口病と網膜色素変性

西口 康二

臨床眼科   75 巻 ( 12 ) 頁： 1453 - 1459   2021年11月

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出版者・発行元：株式会社医学書院  

DOI： 10.11477/mf.1410214208

CiNii Research

Building Cube Method を利用した車体フレームのボクセルトポロジー最適化

和田 有司, 嶋田 宗将, 西口 浩司, 岡澤 重信, 坪倉 誠

日本計算工学会論文集   2021 巻 ( 0 ) 頁： 20210019 - 20210019   2021年11月

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記述言語：日本語   出版者・発行元：一般社団法人 日本計算工学会  

<p>大領域の金属ブロックから自動車フレームの概念構造をトポロジー最適化で得るには、1%以下の体積制約と十分な要素解像度を要する。階層直交格子を利用した有限体積法ソルバであるCUBEフレームワークを、コネクティビティつき直交格子のボクセル有限要素法ソルバとして利用することで、超並列環境でトポロジー最適化の反復解析を実行する。車体フレームを意図した数億要素のトポロジー最適化を実施し、その並列化性能を測定した。</p>

DOI： 10.11421/jsces.2021.20210019

CiNii Research

Bilateral diffuse uveal melanocytic proliferationの1例

新田 文彦, 西口 康二, 國方 彦志, 中澤 徹

眼科臨床紀要   14 巻 ( 11 ) 頁： 771 - 772   2021年11月

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記述言語：日本語   出版者・発行元：眼科臨床紀要会  

Polypoidal choroidal vasculopathy in a case of retinitis pigmentosa, successfully treated with intravitreal aflibercept. 国際誌

Takahashi N, Kunikata H, Yasuda M, Hariya T, Nishiguchi KM, Nakazawa T

American journal of ophthalmology case reports   23 巻   頁： 101123 - 101123   2021年9月

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記述言語：英語  

DOI： 10.1016/j.ajoc.2021.101123

PubMed

MRIにて発見された鉄片異物による遅発性眼内炎の1例

新田 文彦, 國方 彦志, 西口 康二, 阿部 俊明, 中澤 徹

臨床眼科   75 巻 ( 7 ) 頁： 947 - 952   2021年7月

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記述言語：日本語   出版者・発行元：(株)医学書院  

＜文献概要＞目的:金属が体内に留置されている患者にはMRIは禁忌であり,その画像の報告は稀有である。今回,MRI撮影を契機に眼内鉄片異物が発見され,眼内炎に対して硝子体手術を行った症例を経験したので報告する。症例:40歳,男性。コンクリートを鏨で叩く作業中に左眼に違和感を自覚した。受傷翌日,近医を受診したが異常は認めなかった。受傷2ヵ月後に左眼の羞明を自覚し,同近医を再診した。左眼の瞳孔散大を認め,近医神経内科へ紹介となった。MRIにて左眼とその周辺の画像が大きく変形したことから金属片の迷入が疑われ,CTにて左眼内に金属片を認めたため当院に紹介となった。所見:左眼は視力(0.8),前房と水晶体は清明,虹彩は上鼻側が一部萎縮,眼底は軽度硝子体混濁と周辺部を圧迫して観察することで12時方向の鋸状縁付近に金属片を認めた。自覚症状,他覚的所見が乏しいため経過観察としたが,受傷2ヵ月半後に眼内炎を発症したため,硝子体手術と水晶体乳化吸引術(眼内レンズは挿入せず)を施行した。硝子体の培養からはCutibacterium acnesが検出された。抗菌薬の点滴,点眼加療などを行い眼内炎は治癒した。受傷6ヵ月後に眼内レンズを挿入し,左視力(1.2)となった。結論:金属片迷入が否定できない症例へのMRIは慎重に検討し注意すべきであるが,期せずして得られたそのMRI検査では,金属による特徴的な画像を呈することがある。

その他リンク： https://search.jamas.or.jp/default/link?pub_year=2021&ichushi_jid=J01537&link_issn=&doc_id=20210628130019&doc_link_id=10.11477%2Fmf.1410214048&url=https%3A%2F%2Fdoi.org%2F10.11477%2Fmf.1410214048&type=%E5%8C%BB%E6%9B%B8.jp_%E3%82%AA%E3%83%BC%E3%83%AB%E3%82%A2%E3%82%AF%E3%82%BB%E3%82%B9&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00024_2.gif

Regional differences in genes and variants causing retinitis pigmentosa in Japan

Koyanagi, Y; Akiyama, M; Nishiguchi, KM; Momozawa, Y; Kamatani, Y; Takata, S; Inai, C; Iwasaki, Y; Kumano, M; Murakami, Y; Komori, S; Gao, D; Kurata, K; Hosono, K; Ueno, S; Hotta, Y; Murakami, A; Terasaki, H; Wada, Y; Nakazawa, T; Ishibashi, T; Ikeda, Y; Kubo, M; Sonoda, KH

JAPANESE JOURNAL OF OPHTHALMOLOGY   65 巻 ( 3 ) 頁： 338 - 343   2021年5月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japanese Journal of Ophthalmology  

Purpose: To investigate the regional differences in the genes and variants causing retinitis pigmentosa (RP) in Japan Study design: Retrospective multicenter study Methods: In total, 1204 probands of each pedigree clinically diagnosed with nonsyndromic RP were enrolled from 5 Japanese facilities. The regions were divided into the Tohoku region, the Kanto and Chubu regions, and the Kyushu region according to the location of the hospitals where the participants were enrolled. We compared the proportions of the causative genes and the distributions of the pathogenic variants among these 3 regions. Results: The proportions of genetically solved cases were 29.4% in the Tohoku region (n = 500), 29.6% in the Kanto and Chubu regions (n = 196), and 29.7% in the Kyushu region (n = 508), which did not differ statistically (P =.99). No significant regional differences in the proportions of each causative gene in genetically solved patients were observed after correction by multiple testing. Among the 29 pathogenic variants detected in all 3 regions, only p.(Pro347Leu) in RHO was an autosomal dominant variant; the remaining 28 variants were found in autosomal recessive genes. Conversely, 78.6% (275/350) of the pathogenic variants were detected only in a single region, and 6 pathogenic variants (p.[Asn3062fs] in EYS, p.[Ala315fs] in EYS, p.[Arg872fs] in RP1, p.[Ala126Val] in RDH12, p.[Arg41Trp] in CRX, and p.[Gly381fs] in PRPF31) were frequently found in ≥ 4 patients in the single region. Conclusion: We observed region-specific pathogenic variants in the Japanese population. Further investigations of causative genes in multiple regions in Japan will contribute to the expansion of the catalog of genetic variants causing RP.

DOI： 10.1007/s10384-021-00824-w

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Scopus

PubMed

日本網膜色素変性レジストリプロジェクトに登録されたデータの解析

池田 康博, 山本 修一, 村上 晶, 高橋 政代, 川崎 良, 西口 康二, 坂本 泰二, 山下 英俊

日本眼科学会雑誌   125 巻 ( 4 ) 頁： 425 - 430   2021年4月

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記述言語：日本語   出版者・発行元：(公財)日本眼科学会  

目的:網膜色素変性(RP)は現時点で有効な治療法のない難病で,新規の治療法開発が最も切望されている疾患の一つである.RPの基礎研究・治療研究を推進するために立ち上げた,RPおよびその類縁疾患に特化した患者レジストリである日本網膜色素変性レジストリプロジェクト(Japan Retinitis Pigmentosa Registry Project:JRPRP)の現状と登録されたデータの集計結果を報告する.対象と方法:2018年7月〜2020年3月までにJRPRPに登録されたRP患者1,817名の患者背景を検討した.結果:JRPRPに参加している25施設中13施設から登録があった.性別は男性851名,女性966名.年齢は55.0±17.4歳(平均値±標準偏差).病型の内訳は定型RP1,563名,錐体杆体ジストロフィ62名,無色素性RP59名,中心型・傍中心型RP37名,クリスタリン網膜症18名,Usher症候群15名,コロイデレミア14名,その他34名,不明15名であった.遺伝形式は常染色体優性遺伝12.7%,常染色体劣性遺伝18.5%,伴性劣性遺伝1.7%,孤発型64.3%,不明2.9%であった.定型RPの病因遺伝子診断率は44.3%で,EYS遺伝子異常が最も多かった.さらに,EYS遺伝子異常の患者はほかの常染色体劣性遺伝の患者より視力が良好な傾向がみられた.結論:JRPRPに登録された患者の遺伝形式の割合は既報と同程度であった.同様に,病因遺伝子としてはEYS遺伝子異常が最も多いことが分かった.さらなる患者データの集積により,本邦のRP患者の特徴が明らかとなることが予想される.(著者抄録)

A hypomorphic variant in <i>EYS</i> detected by genome-wide association study contributes toward retinitis pigmentosa 国際誌

Nishiguchi, KM; Miya, F; Mori, Y; Fujita, K; Akiyama, M; Kamatani, T; Koyanagi, Y; Sato, K; Takigawa, T; Ueno, S; Tsugita, M; Kunikata, H; Cisarova, K; Nishino, J; Murakami, A; Abe, T; Momozawa, Y; Terasaki, H; Wada, Y; Sonoda, KH; Rivolta, C; Tsunoda, T; Tsujikawa, M; Ikeda, Y; Nakazawa, T

COMMUNICATIONS BIOLOGY   4 巻 ( 1 ) 頁： 140 - 140   2021年1月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Communications Biology  

The genetic basis of Japanese autosomal recessive retinitis pigmentosa (ARRP) remains largely unknown. Herein, we applied a 2-step genome-wide association study (GWAS) in 640 Japanese patients. Meta-GWAS identified three independent peaks at P < 5.0 × 10−8, all within the major ARRP gene EYS. Two of the three were each in linkage disequilibrium with a different low frequency variant (allele frequency < 0.05); a known founder Mendelian mutation (c.4957dupA, p.S1653Kfs*2) and a non-synonymous variant (c.2528 G > A, p.G843E) of unknown significance. mRNA harboring c.2528 G > A failed to restore rhodopsin mislocalization induced by morpholino-mediated knockdown of eys in zebrafish, consistent with the variant being pathogenic. c.2528 G > A solved an additional 7.0% of Japanese ARRP cases. The third peak was in linkage disequilibrium with a common non-synonymous variant (c.7666 A > T, p.S2556C), possibly representing an unreported disease-susceptibility signal. GWAS successfully unraveled genetic causes of a rare monogenic disorder and identified a high frequency variant potentially linked to development of local genome therapeutics.

DOI： 10.1038/s42003-021-01662-9

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Scopus

PubMed

Ocular microcirculation changes, measured with laser speckle flowgraphy and optical coherence tomography angiography, in branch retinal vein occlusion with macular edema treated by ranibizumab. 国際誌

Asano T, Kunikata H, Yasuda M, Nishiguchi KM, Abe T, Nakazawa T

International ophthalmology   41 巻 ( 1 ) 頁： 151 - 162   2021年1月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s10792-020-01562-7

PubMed

EULERIAN FORMULATION USING LAGRANGIAN MARKER PARTICLES WITH REFERENCE MAP TECHNIQUE FOR FLUID-STRUCTURE INTERACTION PROBLEM

Shimada T., Nishiguchi K., Peco C., Okazawa S., Tsubokura M.

9th International Conference on Computational Methods for Coupled Problems in Science and Engineering, COUPLED PROBLEMS 2021     2021年

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出版者・発行元：9th International Conference on Computational Methods for Coupled Problems in Science and Engineering, COUPLED PROBLEMS 2021  

Full Eulerian methods constitute a family of numerical techniques used to simulate fluid-structure interaction problems. In a full Eulerian method, the velocity gradient tensor is used to compute deformation of solid. However, it is difficult to compute solid stress accurately near the interface, where the velocity between fluid and solid changes drastically. In this work, we propose an Eulerian formulation for fluid-structure interaction problems using Lagrangian marker particles with the Reference Map Technique to compute the deformation of solid accurately near material interfaces without using the gradient of the velocity. We illustrate and validate the proposed method through the presentation of various benchmark problems.

Scopus

A case of delayed-onset endophthalmitis due to intraocular iron foreign body detected by MRI

Nitta F., Kunikata H., Nishiguchi K., Abe T., Nakazawa T.

Japanese Journal of Clinical Ophthalmology   75 巻 ( 7 ) 頁： 947 - 952   2021年

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出版者・発行元：Japanese Journal of Clinical Ophthalmology  

Purpose : To report a case of intraocular iron foreign body found by magnetic resonance imaging (MRI). MRI is prohibited in a patient who has metal in the body, and the reports of its images are rare. Case : A 40-year-old man felt pain of the left eye hitting a concrete wall with a chisel. The next day, he underwent a medical examination at the ophthalmology clinic. However, the left eye has no abnormal findings. After 2 months, he noted photophobia on the left eye and presented with mydriasis. The neurologist found intraocular iron foreign body on the left eye by MRI and CT and referred the patient to our clinic. Findings and clinical course : Left visual acuity was 0.8. The anterior chamber and lens were clear. The iris had a part of atrophy. A slightly vitreous opacity and intraocular iron foreign body were found near the ora serrata on the upper side. We selected an observasion because the subjective symptom and the objective symptom were mild. Endophthalmitis occured two and a half months after the accident. Phacoemulsification with aspiration ( without injection of intraocular lens) and vitrectomy were performed on the left eye. Cutibacterium acnes was detected in the culture from the vitreous body. Endophthalmitis was treated by eye drops and intravenous drip of antibiotics. Injection of intraocular lens was performed after 6 months of the accident. Finally, visual acuity recovered to 1.2. Conclusions : MRI should not be perform in a patient who cannot be denied with intraocular iron foreign body. However, we unexpectedlly obtained the characteristic MRI images.

Scopus

Clinical characteristics and high resolution retinal imaging of retinitis pigmentosa caused by <i>RP1</i> gene variants 査読有り

Ueno, S; Koyanagi, Y; Kominami, T; Ito, Y; Kawano, K; Nishiguchi, KM; Rivolta, C; Nakazawa, T; Sonoda, KH; Terasaki, H

JAPANESE JOURNAL OF OPHTHALMOLOGY   64 巻 ( 5 ) 頁： 485 - 496   2020年9月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japanese Journal of Ophthalmology  

Purpose: To report the clinical course and high resolution images of autosomal recessive retinitis pigmentosa (RP) associated with a variant of the RP1 gene (c.4052_4053ins328/p.Tyr1352Alafs*9; m1), a high frequency founder variant in Japanese RP patients. Study design: Retrospective case series. Methods: Nine patients from 5 unrelated Japanese families were studied. Five patients had the m1 variant homozygously, and 4 patients had the m1 variant compound heterozygously with another frameshift variant (c.4196delG/p.Cys1399Leufs*5). Ophthalmic examinations including adaptive optics (AO) fundus imaging were performed periodically. Results: The fundus photographs, fundus autofluorescence (FAF) images, and optical coherence tomographic (OCT) images indicated severe retinal degeneration in all the patients involving the macula even at a young age (20 s). The areas of surviving photoreceptors in the central macula were seen as hyper-autofluorescent regions in the FAF images and preserved outer retinal structure in the OCT images; they were identifiable in the AO fundus images in 8 eyes. The borders of the surviving photoreceptor areas were surrounded by hyporeflective clumps, presumably containing melanin, and the size of these areas decreased progressively during the 4-year follow-up period. The disappearance of the surviving photoreceptor areas was associated with complete blindness. Conclusion: Patients with RP associated with the m1 variant have a progressive and severe retinal degeneration that begins at an early age. Monitoring the surviving photoreceptor areas by AO fundus imaging can provide a more precise pathological record of retinal degeneration.

DOI： 10.1007/s10384-020-00752-1

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PubMed

Companion Diagnosis for Retinal Neuroprotective Treatment by Real-Time Imaging of Calpain Activation Using a Novel Fluorescent Probe. 査読有り 国際誌

Asano T, Nagayo Y, Tsuda S, Ito A, Kobayashi W, Fujita K, Sato K, Nishiguchi KM, Kunikata H, Fujioka H, Kamiya M, Urano Y, Nakazawa T

Bioconjugate chemistry   31 巻 ( 9 ) 頁： 2241 - 2251   2020年8月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1021/acs.bioconjchem.0c00435

PubMed

Effect of the chemical structure on the drug release from brinzolamide based nano eye-drops

Yoshitaka Koseki, Yoshikazu Ikuta, Kota Sato, Shigenobu Aoyagi, Satoshi Inada, Tsunenobu Onodera, Hidetoshi Oikawa, Koji M. Nishiguchi, Toru Nakazawa, Hitoshi Kasai

Molecular Crystals and Liquid Crystals   706 巻 ( 1 ) 頁： 122 - 128   2020年7月

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掲載種別：研究論文（学術雑誌）   出版者・発行元：Informa UK Limited  

DOI： 10.1080/15421406.2020.1743447

Successful surgical outcomes after 23-, 25- and 27-gauge vitrectomy without scleral encircling for giant retinal tear. 査読有り

Kunikata H, Aizawa N, Sato R, Nishiguchi KM, Abe T, Nakazawa T

Japanese journal of ophthalmology   64 巻 ( 5 ) 頁： 506 - 515   2020年7月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s10384-020-00755-y

PubMed

A founder Alu insertion in RP1 gene in Japanese patients with retinitis pigmentosa.

Nishiguchi KM, Fujita K, Ikeda Y, Kunikata H, Koyanagi Y, Akiyama M, Abe T, Wada Y, Sonoda KH, Nakazawa T

Japanese journal of ophthalmology   64 巻 ( 4 ) 頁： 346 - 350   2020年7月

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記述言語：英語  

DOI： 10.1007/s10384-020-00732-5

PubMed

SAG変異を病因とする小口病と網膜色素変性の臨床表現型比較

西口 康二, 池田 康博, 細野 克博, 和田 裕子, 園田 康平, 堀田 喜裕, 村上 晶, 中澤 満, 中澤 徹, 阿部 俊明

眼科臨床紀要   13 巻 ( 7 ) 頁： 490 - 490   2020年7月

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記述言語：日本語   出版者・発行元：眼科臨床紀要会  

RETINAL SENSITIVITY AND VESSEL DENSITY AFTER MACULAR HOLE SURGERY WITH THE SUPERIOR INVERTED INTERNAL LIMITING MEMBRANE FLAP TECHNIQUE. 査読有り 国際誌

Kunikata H, Yasuda M, Aizawa N, Osada U, Nishiguchi KM, Abe T, Nakazawa T

Retina (Philadelphia, Pa.)   41 巻 ( 1 ) 頁： 45 - 53   2020年6月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1097/IAE.0000000000002839

PubMed

Association of retinal vessel density with retinal sensitivity in surgery for idiopathic epiretinal membrane. 査読有り 国際誌

Osada U, Kunikata H, Yasuda M, Hashimoto K, Nishiguchi KM, Nakazawa T

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie   258 巻 ( 9 ) 頁： 1911 - 1920   2020年6月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s00417-020-04754-0

PubMed

Signs of Oguchi Disease and Pigmentary Degeneration from Early in Life. 査読有り 国際誌

Nishiguchi KM, Kunikata H, Nakazawa T

Ophthalmology   127 巻 ( 6 ) 頁： 825 - 825   2020年6月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ophtha.2020.02.020

PubMed

経角膜硝子体切除を併用した眼内レンズ強膜内固定術3症例の検討 査読有り

新田 文彦, 國方 彦志, 大友 孝昭, 西口 康二, 阿部 俊明, 中澤 徹

臨床眼科   74 巻 ( 5 ) 頁： 582 - 588   2020年5月

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記述言語：日本語   出版者・発行元：(株)医学書院  

＜文献概要＞目的:落屑症候群ではチン小帯脆弱のため眼内レンズ強膜内固定術を要することが多いが,長期的には緑内障濾過手術も必要になりやすいため,硝子体切除術を併施する際にも結膜と強膜への侵襲は最小限にとどめたい。今回,眼内レンズ強膜内固定術の際の硝子体切除術を経角膜で行った落屑症候群3症例を報告する。対象と方法:対象は落屑症候群の3症例(男性1例,女性2例,平均年齢84歳)で,中間透光体は白内障2例,眼内レンズ亜脱臼1例であり,いずれにも水晶体振盪があった。術前の矯正視力は0.2〜0.5であった。これらに対して硝子体手術装置を用い経角膜硝子体切除を併施した強膜内固定術(ダブルニードルテクニックを用いたフランジ法)を行った。結果:白内障眼2例の硝子体切除は経角膜的に行うことができた。眼内レンズ亜脱臼の1例では術中に眼内レンズが眼底後極に落下し,後極操作の際に非接触式広角眼底観察システムに硝子体鑷子が干渉したため,経毛様体扁平部硝子体切除術に移行した。最終的に,全例で強膜内固定術を行えた。術後の矯正視力は0.6〜0.9と全例で改善した。全例で一時的に高眼圧(22〜28mmHg)となり,降圧点眼加療を要したことと,軽度の角膜浮腫とデスメ膜皺襞が生じた以外は合併症もなく順調に経過した。結論:将来的に緑内障手術を行う可能性がある落屑症候群などの症例での硝子体切除は,結膜強膜保護の観点から経角膜アプローチは利点があると思われる。現在のサイズの接触レンズや非接触式広角眼底観察システムを用いた手術では後極の操作に難があり,安全,かつスムースに行えない可能性があるため,さらなる工夫が必要と思われた。

経角膜硝子体切除を併用した眼内レンズ強膜内固定術3症例の検討

新田 文彦, 國方 彦志, 大友 孝昭, 西口 康二, 阿部 俊明, 中澤 徹

臨床眼科   74 巻 ( 5 ) 頁： 582 - 588   2020年5月

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記述言語：日本語   出版者・発行元：(株)医学書院  

＜文献概要＞目的:落屑症候群ではチン小帯脆弱のため眼内レンズ強膜内固定術を要することが多いが,長期的には緑内障濾過手術も必要になりやすいため,硝子体切除術を併施する際にも結膜と強膜への侵襲は最小限にとどめたい。今回,眼内レンズ強膜内固定術の際の硝子体切除術を経角膜で行った落屑症候群3症例を報告する。対象と方法:対象は落屑症候群の3症例(男性1例,女性2例,平均年齢84歳)で,中間透光体は白内障2例,眼内レンズ亜脱臼1例であり,いずれにも水晶体振盪があった。術前の矯正視力は0.2〜0.5であった。これらに対して硝子体手術装置を用い経角膜硝子体切除を併施した強膜内固定術(ダブルニードルテクニックを用いたフランジ法)を行った。結果:白内障眼2例の硝子体切除は経角膜的に行うことができた。眼内レンズ亜脱臼の1例では術中に眼内レンズが眼底後極に落下し,後極操作の際に非接触式広角眼底観察システムに硝子体鑷子が干渉したため,経毛様体扁平部硝子体切除術に移行した。最終的に,全例で強膜内固定術を行えた。術後の矯正視力は0.6〜0.9と全例で改善した。全例で一時的に高眼圧(22〜28mmHg)となり,降圧点眼加療を要したことと,軽度の角膜浮腫とデスメ膜皺襞が生じた以外は合併症もなく順調に経過した。結論:将来的に緑内障手術を行う可能性がある落屑症候群などの症例での硝子体切除は,結膜強膜保護の観点から経角膜アプローチは利点があると思われる。現在のサイズの接触レンズや非接触式広角眼底観察システムを用いた手術では後極の操作に難があり,安全,かつスムースに行えない可能性があるため,さらなる工夫が必要と思われた。

その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J01537&link_issn=&doc_id=20200525220013&doc_link_id=10.11477%2Fmf.1410213554&url=https%3A%2F%2Fdoi.org%2F10.11477%2Fmf.1410213554&type=%88%E3%8F%91.jp_%83I%81%5B%83%8B%83A%83N%83Z%83X&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00024_2.gif

Systemic oxidative stress level in patients with central serous chorioretinopathy. 査読有り 国際誌

Kunikata H, Sato R, Nishiguchi KM, Nakazawa T

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie   258 巻 ( 7 ) 頁： 1575 - 1577   2020年4月

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記述言語：英語  

DOI： 10.1007/s00417-020-04664-1

PubMed

Endogenous endophthalmitis caused by group B streptococcus; case reports and review of 35 reported cases. 査読有り 国際誌

Yoshida M, Yokokura S, Nishida T, Mochizuki K, Suzuki T, Maruyama K, Otomo T, Nishiguchi KM, Kunikata H, Nakazawa T

BMC ophthalmology   20 巻 ( 1 ) 頁： 126 - 126   2020年3月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1186/s12886-020-01378-0

PubMed

Genetic Variants Associated With the Onset and Progression of Primary Open-Angle Glaucoma. 査読有り 国際誌

Mabuchi F, Mabuchi N, Sakurada Y, Yoneyama S, Kashiwagi K, Iijima H, Yamagata Z, Takamoto M, Aihara M, Iwata T, Hashimoto K, Sato K, Shiga Y, Nishiguchi KM, Nakazawa T, Akiyama M, Kawase K, Ozaki M, Araie M, Japan Glaucoma Society Omics Group (JGS-OG).

American journal of ophthalmology   215 巻   頁： 135 - 140   2020年3月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ajo.2020.03.014

PubMed

広義原発開放隅角緑内障関連遺伝子領域の多型と酸化ストレスの関係

佐藤 正隆, 橋本 和軌, 志賀 由己浩, 二宮 高洋, 檜森 紀子, 西口 康二, 中澤 徹

日本眼科学会雑誌   124 巻 ( 臨増 ) 頁： 236 - 236   2020年3月

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記述言語：日本語   出版者・発行元：(公財)日本眼科学会  

Association of CRX genotypes and retinal phenotypes confounded by variable expressivity and electronegative electroretinogram. 査読有り 国際誌

Nishiguchi KM, Kunikata H, Fujita K, Hashimoto K, Koyanagi Y, Akiyama M, Ikeda Y, Momozawa Y, Sonoda KH, Murakami A, Wada Y, Nakazawa T

Clinical & experimental ophthalmology   48 巻 ( 5 ) 頁： 644 - 657   2020年2月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/ceo.13743

PubMed

Serum anti-recoverin antibodies is found in elderly patients with retinitis pigmentosa and cancer. 査読有り 国際誌

Sato T, Nishiguchi KM, Fujita K, Miya F, Inoue T, Sasaki E, Asano T, Tsuda S, Shiga Y, Kunikata H, Nakazawa M, Nakazawa T

Acta ophthalmologica   98 巻 ( 6 ) 頁： e722-e729 - e729   2020年2月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/aos.14373

PubMed

Single AAV-mediated mutation replacement genome editing in limited number of photoreceptors restores vision in mice. 査読有り 国際誌

Nishiguchi KM, Fujita K, Miya F, Katayama S, Nakazawa T

Nature communications   11 巻 ( 1 ) 頁： 482 - 482   2020年1月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/s41467-019-14181-3

PubMed

Exploring the Novel Susceptibility Gene Variants for Primary Open-Angle Glaucoma in East Asian Cohorts: The GLAU-GENDISK Study. 査読有り

Kim YW, Kim YJ, Cheong HS, Shiga Y, Hashimoto K, Song YJ, Kim SH, Choi HJ, Nishiguchi KM, Kawai Y, Nagasaki M, Nakazawa T, Park KH, Kim DM, Jeoung JW

Scientific reports   10 巻 ( 1 ) 頁： 221   2020年1月

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DOI： 10.1038/s41598-019-57066-7

PubMed

Progression from Classical Oguchi Disease to Retinitis Pigmentosa after 50 Years.

Nishiguchi KM, Oguchi Y, Nakazawa T

Ophthalmology   127 巻 ( 1 ) 頁： 51   2020年1月

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記述言語：英語  

DOI： 10.1016/j.ophtha.2019.09.015

PubMed

Progression from classical Oguchi disease to retinitis pigmentosa after 50 years 査読有り

Nishiguchi KM, Oguchi Y, Nakazawa T

Ophthalmology   in press 巻   2020年

Phenotypic Features of Oguchi Disease and Retinitis Pigmentosa in Patients with S-Antigen Mutations: A Long-Term Follow-up Study. 査読有り 国際誌

Nishiguchi KM, Ikeda Y, Fujita K, Kunikata H, Akiho M, Hashimoto K, Hosono K, Kurata K, Koyanagi Y, Akiyama M, Suzuki T, Kawasaki R, Wada Y, Hotta Y, Sonoda KH, Murakami A, Nakazawa M, Nakazawa T, Abe T

Ophthalmology   126 巻 ( 11 ) 頁： 1557 - 1566   2019年11月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ophtha.2019.05.027

PubMed

In vivo imaging of the light response in mouse retinal ganglion cells based on a neuronal activity-dependent promoter. 査読有り

Fujita K, Nishiguchi KM, Sato K, Nakagawa Y, Nakazawa T

Biochemical and biophysical research communications   521 巻 ( 2 ) 頁： 471 - 477   2019年10月

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記述言語：英語  

DOI： 10.1016/j.bbrc.2019.10.155

PubMed

Genetic characteristics of retinitis pigmentosa in 1204 Japanese patients 査読有り

Koyanagi, Y; Akiyama, M; Nishiguchi, KM; Momozawa, Y; Kamatani, Y; Takata, S; Inai, C; Iwasaki, Y; Kumano, M; Murakami, Y; Omodaka, K; Abe, T; Komori, S; Gao, D; Hirakata, T; Kurata, K; Hosono, K; Ueno, S; Hotta, Y; Murakami, A; Terasaki, H; Wada, Y; Nakazawa, T; Ishibashi, T; Ikeda, Y; Kubo, M; Sonoda, KH

JOURNAL OF MEDICAL GENETICS   56 巻 ( 10 ) 頁： 662 - 670   2019年10月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Journal of Medical Genetics  

Background The genetic profile of retinitis pigmentosa (RP) in East Asian populations has not been well characterised. Therefore, we conducted a large-scale sequencing study to investigate the genes and variants causing RP in a Japanese population. Methods A total of 1209 Japanese patients diagnosed with typical RP were enrolled. We performed deep resequencing of 83 known causative genes of RP using next-generation sequencing. We defined pathogenic variants as those that were putatively deleterious or registered as pathogenic in the Human Gene Mutation Database or ClinVar database and had a minor allele frequency in any ethnic population of ≤0.5% for recessive genes or ≤0.01% for dominant genes as determined using population-based databases. Results We successfully sequenced 1204 patients with RP and determined 200 pathogenic variants in 38 genes as the cause of RP in 356 patients (29.6%). Variants in six genes (EYS, USH2A, RP1L1, RHO, RP1 and RPGR) caused RP in 65.4% (233/356) of those patients. Among autosomal recessive genes, two known founder variants in EYS [p.(Ser1653fs) and p.(Tyr2935∗)] and four East Asian-specific variants [p.(Gly2752Arg) in USH2A, p.(Arg658∗) in RP1L1, p.(Gly2186Glu) in EYS and p.(Ile535Asn) in PDE6B] and p.(Cys934Trp) in USH2A were found in ≥10 patients. Among autosomal dominant genes, four pathogenic variants [p.(Pro347Leu) in RHO, p.(Arg872fs) in RP1, p.(Arg41Trp) in CRX and p.(Gly381fs) in PRPF31] were found in ≥4 patients, while these variants were unreported or extremely rare in both East Asian and non-East Asian population-based databases. Conclusions East Asian-specific variants in causative genes were the major causes of RP in the Japanese population.

DOI： 10.1136/jmedgenet-2018-105691

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開放隅角緑内障1家系におけるOPTN遺伝子の新規N51T変異の同定

前川 重人, 橋本 和軌, 志賀 由己浩, 津田 聡, 面高 宗子, 横山 悠, 西口 康二, 中澤 徹, 日本緑内障学会遺伝子研究班

日本緑内障学会抄録集   30回 巻   頁： 184 - 184   2019年9月

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記述言語：日本語   出版者・発行元：日本緑内障学会  

A frequent variant in the Japanese population determines quasi-Mendelian inheritance of rare retinal ciliopathy 査読有り 国際誌

Nikopoulos, K; Cisarova, K; Quinodoz, M; Koskiniemi-Kuendig, H; Miyake, N; Farinelli, P; Rehman, AU; Khan, MI; Prunotto, A; Akiyama, M; Kamatani, Y; Terao, C; Miya, F; Ikeda, Y; Ueno, S; Fuse, N; Murakami, A; Wada, Y; Terasaki, H; Sonoda, KH; Ishibashi, T; Kubo, M; Cremers, FPM; Kutalik, Z; Matsumoto, N; Nishiguchi, KM; Nakazawa, T; Rivolta, C

NATURE COMMUNICATIONS   10 巻 ( 1 ) 頁： 2884 - 2884   2019年6月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Nature Communications  

Hereditary retinal degenerations (HRDs) are Mendelian diseases characterized by progressive blindness and caused by ultra-rare mutations. In a genomic screen of 331 unrelated Japanese patients, we identify a disruptive Alu insertion and a nonsense variant (p.Arg1933*) in the ciliary gene RP1, neither of which are rare alleles in Japan. p.Arg1933* is almost polymorphic (frequency = 0.6%, amongst 12,000 individuals), does not cause disease in homozygosis or heterozygosis, and yet is significantly enriched in HRD patients (frequency = 2.1%, i.e., a 3.5-fold enrichment; p-value = 9.2 × 10−5). Familial co-segregation and association analyses show that p.Arg1933* can act as a Mendelian mutation in trans with the Alu insertion, but might also associate with disease in combination with two alleles in the EYS gene in a non-Mendelian pattern of heredity. Our results suggest that rare conditions such as HRDs can be paradoxically determined by relatively common variants, following a quasi-Mendelian model linking monogenic and complex inheritance.

DOI： 10.1038/s41467-019-10746-4

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Quantitative analysis of the macula with optical coherence tomography angiography in normal Japanese subjects: The Taiwa Study. 査読有り 国際誌

Sato R, Kunikata H, Asano T, Aizawa N, Kiyota N, Shiga Y, Nishiguchi KM, Kato K, Nakazawa T

Scientific reports   9 巻 ( 1 ) 頁： 8875 - 8875   2019年6月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/s41598-019-45336-3

PubMed

Correlation between aqueous flare and residual visual field area in retinitis pigmentosa. 査読有り 国際誌

Nishiguchi KM, Yokoyama Y, Kunikata H, Abe T, Nakazawa T

The British journal of ophthalmology   103 巻 ( 4 ) 頁： 475 - 480   2019年4月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1136/bjophthalmol-2018-312225

PubMed

Macular Dystrophy and Cone-Rod Dystrophy Caused by Mutations in the RP1 Gene: Extending the RP1 Disease Spectrum. 査読有り

Verbakel SK, van Huet RAC, den Hollander AI, Geerlings MJ, Kersten E, Klevering BJ, Klaver CCW, Plomp AS, Wesseling NL, Bergen AAB, Nikopoulos K, Rivolta C, Ikeda Y, Sonoda KH, Wada Y, Boon CJF, Nakazawa T, Hoyng CB, Nishiguchi KM

Investigative ophthalmology & visual science   60 巻 ( 4 ) 頁： 1192 - 1203   2019年3月

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DOI： 10.1167/iovs.18-26084

PubMed

Macular degeneration as a common cause of visual loss in spinocerebellar ataxia type 1 (SCA1) patients. 査読有り

Nishiguchi KM, Aoki M, Nakazawa T, Abe T

Ophthalmic genetics   40 巻 ( 1 ) 頁： 49 - 53   2019年2月

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DOI： 10.1080/13816810.2019.1571614

PubMed

Alternative methods to detect anti-TRPM1 antibodies. 査読有り 国際誌

Nishiguchi KM, Fujita K, Inoue T, Nakazawa T

Clinical & experimental ophthalmology   47 巻 ( 1 ) 頁： 148 - 149   2019年1月

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記述言語：英語  

DOI： 10.1111/ceo.13365

PubMed

Retained Plasticity and Substantial Recovery of Rod-Mediated Visual Acuity at the Visual Cortex in Blind Adult Mice with Retinal Dystrophy 査読有り

Nishiguchi, KM; Fujita, K; Tokashiki, N; Komamura, H; Takemoto-Kimura, S; Okuno, H; Bito, H; Nakazawa, T

MOLECULAR THERAPY   26 巻 ( 10 ) 頁： 2397 - 2406   2018年10月

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記述言語：英語   出版者・発行元：Molecular Therapy  

In patients born blind with retinal dystrophies, understanding the critical periods of cortical plasticity is important for successful visual restoration. In this study, we sought to model childhood blindness and investigate the plasticity of visual pathways. To this end, we generated double-mutant (Pde6ccpfl1/cpfl1Gnat1IRD2/IRD2) mice with absent rod and cone photoreceptor function, and we evaluated their response for restoring rod (GNAT1) function through gene therapy. Despite the limited effectiveness of gene therapy in restoring visual acuity in patients with retinal dystrophy, visual acuity was, unexpectedly, successfully restored in the mice at the level of the primary visual cortex in this study. This success in visual restoration, defined by changes in the quantified optokinetic response and pattern visually evoked potential, was achieved regardless of the age at treatment (up to 16 months). In the contralateral visual cortex, cortical plasticity, tagged with light-triggered transcription of Arc, was also restored after the treatment in blind mice carrying an Arc promoter-driven reporter gene, dVenus. Our results demonstrate the remarkable plasticity of visual circuits for one of the two photoreceptor mechanisms in older as well as younger mice with congenital blindness due to retinal dystrophies. Nishiguchi et al. show that recovery of rod-mediated visual acuity is possible at the level of the visual cortex after functional restoration of the rods, even in adults. The remarkable plasticity of visual circuits shown provides an important insight into the restoration of vision.

DOI： 10.1016/j.ymthe.2018.07.012

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Reliable detection of low visual acuity in mice with pattern visually evoked potentials. 査読有り

Tokashiki N, Nishiguchi KM, Fujita K, Sato K, Nakagawa Y, Nakazawa T

Scientific reports   8 巻 ( 1 ) 頁： 15948   2018年10月

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DOI： 10.1038/s41598-018-34413-8

PubMed

Metabolomic changes in the mouse retina after optic nerve injury. 査読有り 国際誌

Sato K, Saigusa D, Saito R, Fujioka A, Nakagawa Y, Nishiguchi KM, Kokubun T, Motoike IN, Maruyama K, Omodaka K, Shiga Y, Uruno A, Koshiba S, Yamamoto M, Nakazawa T

Scientific reports   8 巻 ( 1 ) 頁： 11930 - 11930   2018年8月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/s41598-018-30464-z

PubMed

Ecel1 Knockdown With an AAV2-Mediated CRISPR/Cas9 System Promotes Optic Nerve Damage-Induced RGC Death in the Mouse Retina. 査読有り 国際誌

Sato K, Shiga Y, Nakagawa Y, Fujita K, Nishiguchi KM, Tawarayama H, Murayama N, Maekawa S, Yabana T, Omodaka K, Katayama S, Feng Q, Tsuda S, Nakazawa T

Investigative ophthalmology & visual science   59 巻 ( 10 ) 頁： 3943 - 3951   2018年8月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1167/iovs.18-23784

PubMed

Prevalence of anti-retinal antibodies in epiretinal membranes and macular holes. 査読有り 国際誌

Kunikata H, Fujita K, Nishiguchi KM, Nakazawa T

Clinical & experimental ophthalmology   46 巻 ( 5 ) 頁： 556 - 558   2018年7月

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記述言語：英語  

DOI： 10.1111/ceo.13099

PubMed

Levels of Anti-Retinal Antibodies in Retinal Detachment and Proliferative Vitreoretinopathy. 査読有り 国際誌

Ichinohasama R, Nishiguchi KM, Fujita K, Aizawa N, Inoue T, Sasaki E, Kunikata H, Nakazawa T

Current eye research   43 巻 ( 6 ) 頁： 804 - 809   2018年6月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/02713683.2018.1451544

PubMed

Anti-TRPM1 antibodies in patients with retinal degeneration. 査読有り 国際誌

Nishiguchi KM, Fujita K, Inoue T, Nakazawa T

Clinical & experimental ophthalmology   46 巻 ( 9 ) 頁： 1087 - 1089   2018年6月

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記述言語：英語  

DOI： 10.1111/ceo.13341

PubMed

Genome-wide association study identifies seven novel susceptibility loci for primary open-angle glaucoma 査読有り

Shiga, Y; Akiyama, M; Nishiguchi, KM; Sato, K; Shimozawa, N; Takahashi, A; Momozawa, Y; Hirata, M; Matsuda, K; Yamaji, T; Iwasaki, M; Tsugane, S; Oze, I; Mikami, H; Naito, M; Wakai, K; Yoshikawa, M; Miyake, M; Yamashiro, K; Kashiwagi, K; Iwata, T; Mabuchi, F; Takamoto, M; Ozaki, M; Kawase, K; Aihara, M; Araie, M; Yamamoto, T; Kiuchi, Y; Nakamura, M; Ikeda, Y; Sonoda, KH; Ishibashi, T; Nitta, K; Iwase, A; Shirato, S; Oka, Y; Satoh, M; Sasaki, M; Fuse, N; Suzuki, Y; Cheng, CY; Khor, CC; Baskaran, M; Perera, S; Aung, T; Vithana, EN; Bailey, JNC; Kang, JH; Pasquale, LR; Haines, JL; Wiggs, JL; Burdon, KP; Gharahkhani, P; Hewitt, AW; Mackey, DA; MacGregor, S; Craig, JE; Allingham, RR; Hauser, M; Ashaye, A; Budenz, DL; Akafo, S; Williams, SEI; Kamatani, Y; Nakazawa, T; Kubo, M

HUMAN MOLECULAR GENETICS   27 巻 ( 8 ) 頁： 1486 - 1496   2018年4月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Human Molecular Genetics  

Primary open-angle glaucoma (POAG) is the leading cause of irreversible blindness worldwide for which 15 diseaseassociated loci had been discovered. Among them, only 5 loci have been associated with POAG in Asians. We carried out a genome-wide association study and a replication study that included a total of 7378 POAG cases and 36 385 controls from a Japanese population. After combining the genome-wide association study and the two replication sets, we identified 11 POAG-associated loci, including 4 known (CDKN2B-AS1, ABCA1, SIX6 and AFAP1) and 7 novel loci (FNDC3B, ANKRD55-MAP3K1, LMX1B, LHPP, HMGA2, MEIS2 and LOXL1) at a genome-wide significance level (P<5.0×10-8), bringing the total number of POAG-susceptibility loci to 22. The 7 novel variants were subsequently evaluated in a multiethnic population comprising non-Japanese East Asians (1008 cases, 591 controls), Europeans (5008 cases, 35 472 controls) and Africans (2341 cases, 2037 controls). The candidate genes located within the new loci were related to ocular development (LMX1B, HMGA2 and MAP3K1) and glaucoma-related phenotypes (FNDC3B, LMX1B and LOXL1). Pathway analysis suggested epidermal growth factor receptor signaling might be involved in POAG pathogenesis. Genetic correlation analysis revealed the relationships between POAG and systemic diseases, including type 2 diabetes and cardiovascular diseases. These results improve our understanding of the genetic factors that affect the risk of developing POAG and provide new insight into the genetic architecture of POAG in Asians.

DOI： 10.1093/hmg/ddy053

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Discovery of a Cynomolgus Monkey Family With Retinitis Pigmentosa. 査読有り 国際誌

Ikeda Y, Nishiguchi KM, Miya F, Shimozawa N, Funatsu J, Nakatake S, Fujiwara K, Tachibana T, Murakami Y, Hisatomi T, Yoshida S, Yasutomi Y, Tsunoda T, Nakazawa T, Ishibashi T, Sonoda KH

Investigative ophthalmology & visual science   59 巻 ( 2 ) 頁： 826 - 830   2018年2月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1167/iovs.17-22958

PubMed

Quantitative MRI evaluation of glaucomatous changes in the visual pathway. 査読有り 国際誌

Fukuda M, Omodaka K, Tatewaki Y, Himori N, Matsudaira I, Nishiguchi KM, Murata T, Taki Y, Nakazawa T

PloS one   13 巻 ( 7 ) 頁： e0197027   2018年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1371/journal.pone.0197027

PubMed

The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation. 査読有り

Maekawa S, Sato K, Fujita K, Daigaku R, Tawarayama H, Murayama N, Moritoh S, Yabana T, Shiga Y, Omodaka K, Maruyama K, Nishiguchi KM, Nakazawa T

Scientific reports   7 巻 ( 1 ) 頁： 6885   2017年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/s41598-017-06969-4

Scopus

PubMed

Genetic analysis of Japanese primary open-angle glaucoma patients and clinical characterization of risk alleles near CDKN2B-AS1, SIX6 and GAS7. 査読有り

Shiga Y, Nishiguchi KM, Kawai Y, Kojima K, Sato K, Fujita K, Takahashi M, Omodaka K, Araie M, Kashiwagi K, Aihara M, Iwata T, Mabuchi F, Takamoto M, Ozaki M, Kawase K, Fuse N, Yamamoto M, Yasuda J, Nagasaki M, Nakazawa T, Japan Glaucoma Society Omics Group (JGS-OG).

PloS one   12 巻 ( 12 ) 頁： e0186678   2017年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1371/journal.pone.0186678

Scopus

PubMed

Optic nerve head microcirculation in autosomal dominant optic atrophy and normal-tension glaucoma. 査読有り

Himori N, Kunikata H, Inoue M, Takeshita T, Nishiguchi KM, Nakazawa T

Acta ophthalmologica   95 巻 ( 8 ) 頁： e799 - e800   2017年12月

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記述言語：英語  

DOI： 10.1111/aos.13353

Web of Science

PubMed

Additive effects of genetic variants associated with intraocular pressure in primary open-angle glaucoma. 査読有り

Mabuchi F, Mabuchi N, Sakurada Y, Yoneyama S, Kashiwagi K, Iijima H, Yamagata Z, Takamoto M, Aihara M, Iwata T, Kawase K, Shiga Y, Nishiguchi KM, Nakazawa T, Ozaki M, Araie M, Japan Glaucoma Society Omics Group (JGS-OG).

PloS one   12 巻 ( 8 ) 頁： e0183709   2017年8月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1371/journal.pone.0183709

Web of Science

PubMed

Spatially and Temporally Regulated <i>NRF2</i> Gene Therapy Using <i>Mcp-1</i> Promoter in Retinal Ganglion Cell Injury. 査読有り

Fujita K, Nishiguchi KM, Shiga Y, Nakazawa T

Molecular therapy. Methods & clinical development   5 巻   頁： 130 - 141   2017年6月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.omtm.2017.04.003

Web of Science

PubMed

The neuroprotective effect of latanoprost acts via klotho-mediated suppression of calpain activation after optic nerve transection. 査読有り

Yamamoto K, Sato K, Yukita M, Yasuda M, Omodaka K, Ryu M, Fujita K, Nishiguchi KM, Machida S, Nakazawa T

Journal of neurochemistry   140 巻 ( 3 ) 頁： 495 - 508   2017年2月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/jnc.13902

Web of Science

PubMed

Brimonidine Enhances the Electrophysiological Response of Retinal Ganglion Cells through the Trk-MAPK/ERK and PI3K Pathways in Axotomized Eyes. 査読有り

Yukita M, Omodaka K, Machida S, Yasuda M, Sato K, Maruyama K, Nishiguchi KM, Nakazawa T

Current eye research   42 巻 ( 1 ) 頁： 125 - 133   2017年1月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3109/02713683.2016.1153112

Web of Science

PubMed

Whole Genome Sequencing in Patients with Retinitis Pigmentosa

Nishiguchi, KM

ADVANCES IN VISION RESEARCH, VOL I: GENETIC EYE RESEARCH IN ASIA AND THE PACIFIC     頁： 83 - 91   2017年

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DOI： 10.1007/978-4-431-56511-6_8

Web of Science