Updated on 2024/03/19

写真a

 
OHASHI Naoki
 
Organization
Nagoya University Hospital Associate professor of hospital
Title
Associate professor of hospital

Degree 1

  1. 博士(医学) ( 2008.11   名古屋大学 ) 

Research Areas 1

  1. Life Science / Embryonic medicine and pediatrics

 

Papers 5

  1. Dysbiosis of gut microbiota in patients with protein-losing enteropathy after the Fontan procedure

    Go, K; Horiba, K; Yamamoto, H; Morimoto, Y; Fukasawa, Y; Ohashi, N; Yasuda, K; Ishikawa, Y; Kuraishi, K; Suzuki, K; Ito, Y; Takahashi, Y; Kato, T

    INTERNATIONAL JOURNAL OF CARDIOLOGY   Vol. 396   page: 131554   2024.2

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    Language:English   Publisher:International Journal of Cardiology  

    Background: There is a lack of predictive biomarkers for the onset or activity of protein-losing enteropathy (PLE), a Fontan procedure-associated complication. Here, we aimed to identify the gut microbiota composition of patients with active PLE and investigate its relationship with PLE activity. Methods: This multicenter case–control study involved patients who developed PLE (n = 16) after the Fontan procedure and those who did not (non-PLE; n = 20). Patients with PLE who maintained a serum albumin level of ≥3 g/dL for >1 year were included in the remissive-stage-PLE group (n = 9) and those who did not maintain this level were included in the active-PLE group (n = 7). 16S rRNA gene sequencing analysis of fecal samples was performed using QIIME2 pipeline. Alpha (Shannon and Faith's phylogenetic diversity indices) and beta diversity was assessed using principal coordinate analysis based on unweighted UniFrac distances. Results: Shannon and Faith's phylogenetic diversity indices were lower in the active-PLE group than in the remissive-stage- (q = 0.028 and 0.025, respectively) and non-PLE (q = 0.028 and 0.017, respectively) groups. Analysis of beta diversity revealed a difference in the microbiota composition between the active-PLE and the other two groups. Linear discriminant effect size analysis demonstrated differences in the relative abundance of Bifidobacterium and Granulicatella spp., and Ruminococcus torques between patients with active- and those with remissive-stage-PLE. Conclusions: Gut microbiota dysbiosis was observed in patients with active PLE. Changes in the bacterial composition of the gut microbiota and decreased diversity may be associated with the severity of PLE.

    DOI: 10.1016/j.ijcard.2023.131554

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  2. (, 10.32388/74e1v5)

    Chida-Nagai A., Masaki N., Maeda K., Sasaki K., Sato H., Muneuchi J., Ochiai Y., Murayama H., Tahara M., Shiono A., Shinozuka A., Kono F., Machida D., Toyooka S., Sugimoto S., Nakamura K., Akagi S., Kondo M., Kasahara S., Kotani Y., Koizumi J., Oda K., Harada M., Nakajima D., Murata A., Nagata H., Yatsunami K., Kobayashi T., Matsunaga Y., Inoue T., Yamagishi H., Nakagawa N., Ohtani K., Yamamoto M., Ito Y., Hokosaki T., Kuwahara Y., Masutani S., Nomura K., Wada T., Sawada H., Abiko M., Takahashi T., Ishikawa Y., Okada S., Naitoh A., Toda T., Ando T., Masuzawa A., Hoshino S., Kawada M., Nomura Y., Ueno K., Ohashi N., Tachibana T., Cao Y., Ueda H., Yanagi S., Koide M., Mitsushita N., Higashi K., Minosaki Y., Hayashi T., Okamoto T., Kuraishi K., Ehara E., Ishida H., Horigome H., Murakami T., Takei K., Ishii T., Harada G., Hirata Y., Maeda J., Tatebe S., Ota C., Hayabuchi Y., Sakazaki H., Sasaki T., Hirono K., Suzuki S., Yasuda M., Takeda A., Sawada M., Miyaji K., Kitagawa A., Nakai Y., Kakimoto N., Agematsu K., Manabe A., Saiki Y.

    Frontiers in Cardiovascular Medicine   Vol. 11   2024

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    Publisher:Frontiers in Cardiovascular Medicine  

    In the published article, an author name was incorrectly written as Madoka Sawai. The correct spelling is Madoka Sawada. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

    DOI: 10.3389/fcvm.2024.1369831

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  3. Use of the index of pulmonary vascular disease for predicting long-term outcome of pulmonary arterial hypertension associated with congenital heart disease

    Chida-Nagai A., Masaki N., Maeda K., Sasaki K., Sato H., Muneuchi J., Ochiai Y., Murayama H., Tahara M., Shiono A., Shinozuka A., Kono F., Machida D., Toyooka S., Sugimoto S., Nakamura K., Akagi S., Kondo M., Kasahara S., Kotani Y., Koizumi J., Oda K., Harada M., Nakajima D., Murata A., Nagata H., Yatsunami K., Kobayashi T., Matsunaga Y., Inoue T., Yamagishi H., Nakagawa N., Ohtani K., Yamamoto M., Ito Y., Hokosaki T., Kuwahara Y., Masutani S., Nomura K., Wada T., Sawada H., Abiko M., Takahashi T., Ishikawa Y., Okada S., Naitoh A., Toda T., Ando T., Masuzawa A., Hoshino S., Kawada M., Nomura Y., Ueno K., Ohashi N., Tachibana T., Cao Y., Ueda H., Yanagi S., Koide M., Mitsushita N., Higashi K., Minosaki Y., Hayashi T., Okamoto T., Kuraishi K., Ehara E., Ishida H., Horigome H., Murakami T., Takei K., Ishii T., Harada G., Hirata Y., Maeda J., Tatebe S., Ota C., Hayabuchi Y., Sakazaki H., Sasaki T., Hirono K., Suzuki S., Yasuda M., Takeda A., Sawai M., Miyaji K., Kitagawa A., Nakai Y., Kakimoto N., Agematsu K., Manabe A., Saiki Y.

    Frontiers in Cardiovascular Medicine   Vol. 10   2023

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    Publisher:Frontiers in Cardiovascular Medicine  

    Aims: Limited data exist on risk factors for the long-term outcome of pulmonary arterial hypertension (PAH) associated with congenital heart disease (CHD-PAH). We focused on the index of pulmonary vascular disease (IPVD), an assessment system for pulmonary artery pathology specimens. The IPVD classifies pulmonary vascular lesions into four categories based on severity: (1) no intimal thickening, (2) cellular thickening of the intima, (3) fibrous thickening of the intima, and (4) destruction of the tunica media, with the overall grade expressed as an additive mean of these scores. This study aimed to investigate the relationship between IPVD and the long-term outcome of CHD-PAH. Methods: This retrospective study examined lung pathology images of 764 patients with CHD-PAH aged <20 years whose lung specimens were submitted to the Japanese Research Institute of Pulmonary Vasculature for pulmonary pathological review between 2001 and 2020. Clinical information was collected retrospectively by each attending physician. The primary endpoint was cardiovascular death. Results: The 5-year, 10-year, 15-year, and 20-year cardiovascular death-free survival rates for all patients were 92.0%, 90.4%, 87.3%, and 86.1%, respectively. The group with an IPVD of ≥2.0 had significantly poorer survival than the group with an IPVD <2.0 (P =.037). The Cox proportional hazards model adjusted for the presence of congenital anomaly syndromes associated with pulmonary hypertension, and age at lung biopsy showed similar results (hazard ratio 4.46; 95% confidence interval: 1.45–13.73; P =.009). Conclusions: The IPVD scoring system is useful for predicting the long-term outcome of CHD-PAH. For patients with an IPVD of ≥2.0, treatment strategies, including choosing palliative procedures such as pulmonary artery banding to restrict pulmonary blood flow and postponement of intracardiac repair, should be more carefully considered.

    DOI: 10.3389/fcvm.2023.1212882

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  4. TREC/KREC Newborn Screening followed by Next-Generation Sequencing for Severe Combined Immunodeficiency in Japan

    Wakamatsu, M; Kojima, D; Muramatsu, H; Okuno, Y; Kataoka, S; Nakamura, F; Sakai, Y; Tsuge, I; Ito, T; Ueda, K; Saito, A; Morihana, E; Ito, Y; Ohashi, N; Tanaka, M; Tanaka, T; Kojima, S; Nakajima, Y; Ito, T; Takahashi, Y

    JOURNAL OF CLINICAL IMMUNOLOGY   Vol. 42 ( 8 ) page: 1696 - 1707   2022.11

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    Language:English   Publisher:Journal of Clinical Immunology  

    Purpose: The aim of this study is to evaluate the usefulness of T cell receptor excision circle (TREC) and/or kappa-deleting recombination excision circle (KREC) measurements integrated with diagnostic next-generation sequencing (NGS) analysis using a severe combined immunodeficiency (SCID) newborn screening (NBS) program. Methods: TREC and/or KREC values were measured in 137,484 newborns between April 2017 and December 2021 using EnLite TREC (n = 80,791) or TREC/KREC kits (n = 56,693). For newborns with positive screening results, diagnostic NGS analysis was performed with a 349-gene panel to detect genetic mutations associated with primary immunodeficiencies (PIDs). Results: A total of 145 newborns (0.11%) had abnormal TREC and/or KREC values, and a genetic diagnosis was established in 2 patients with SCID (1 in 68,742 newborns) (IL2RG-SCID and reticular dysgenesis) and 10 with non-SCID PIDs with T and/or B cell deficiencies (1 in 13,748 newborns) using NGS analysis. Furthermore, TREC values of 2849 newborns were measured and confirmed the significant correlation between the results of both TREC and TREC/KREC kits (P < 0.001) and naïve T cell counts. Conclusions: We performed the first large-scale TREC and TREC/KREC NBS programs in Japan. Our NBS programs followed by the diagnostic NGS analysis for newborns with abnormal TREC and/or KREC values are useful for the early identification and rapid molecular evaluation of not only SCID but also different non-SCID PIDs.

    DOI: 10.1007/s10875-022-01335-0

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  5. Conception by assisted reproductive technology in infants with critical congenital heart disease in Japan

    Morimoto, Y; Go, K; Yamamoto, H; Fukasawa, Y; Nakai, M; Morihana, E; Yasuda, K; Nishikawa, H; Ohashi, N; Takahashi, Y; Kato, T

    REPRODUCTIVE BIOMEDICINE ONLINE   Vol. 44 ( 1 ) page: 163 - 170   2022.1

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    Language:English   Publisher:Reproductive BioMedicine Online  

    Research question: What is the proportion of infants born as a result of assisted reproductive technology ART across different types of neonatal critical congenital heart disease (CCHD) in a Japanese population? Design: A retrospective analysis of 418 consecutive infants with CCHD that required catheter treatment or surgery within the first 28 days of life or ductal-dependent lesions, in two paediatric centres in Japan, between January 2014 and December 2019. The proportion of ART in infants with each type of CCHD was evaluated. The proportion of ART in infants with univentricular heart defect (UVH) compared with those with biventricular heart defect (BVH) was evaluated. Results: The study group included 229 boys and 189 girls, with a gestational age of 38 ± 2 weeks. Overall, 61 infants (14.6%) were conceived by fertility treatment with 46 (11.0%) conceived by ART. Univentricular heart defect and BVH were identified in 111 infants (26.6%) and 307 infants (73.4%), respectively. The proportion of infants conceived by ART was significantly higher in UVH (16.2%) than in BVH (9.1%) (OR 2.28, 95% CI 1.11 to 4.68, P = 0.025), regardless of maternal age and maternal history of miscarriage. Conclusions: The proportion of ART in infants with CCHD, especially UVH, was high. These findings could form the basis of a rationale for carrying out fetal echocardiography in fetuses conceived by ART.

    DOI: 10.1016/j.rbmo.2021.10.005

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Presentations 2

  1. ASDが見落とされた学校心電図所見を考える

    第27回日本小児心電学会学術集会  2023.12.8 

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    Event date: 2023.12

    Presentation type:Oral presentation (general)  

  2. フォンタン手術後、接合部認める症例の臨床像と問題点

    第59回日本小児循環器学会総会・学術集会  2023.7.8 

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    Event date: 2023.7

    Presentation type:Oral presentation (general)  

KAKENHI (Grants-in-Aid for Scientific Research) 1

  1. ゲノム編集マウスを用いたミオシン遺伝子異常による不整脈発症メカニズムの解明

    Grant number:23K19584  2023.8 - 2025.3

    科学研究費助成事業  研究活動スタート支援

    大橋 直樹

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    Authorship:Principal investigator 

    Grant amount:\2730000 ( Direct Cost: \2100000 、 Indirect Cost:\630000 )

    Wolff-Parkinson-White(以下、WPW)症候群は頻度の高い不整脈疾患である。ほとんどは孤発例だが稀に家族例が存在する。原因遺伝子としてPRKAG2、MYH7が知られているが、両遺伝子に病的バリアントを有さない家族例も報告されており未知の原因遺伝子の存在が示唆される。
    研究代表者は近年、遺伝性WPW症候群を呈する家系においてミオシン関連遺伝子の病的バリアントを発見した。同遺伝子の病的バリアントによりWPW症候群を発症した報告はなく、新規表現型である可能性が高い。
    本研究の目的は、該当バリアントを有するノックインマウスを用いて、WPW症候群の発症メカニズムを解明することである。

 

Teaching Experience (On-campus) 1

  1. 成育医療

    2023

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    新生児から成人まで関わる子どもの病気“小児循環器病”