Updated on 2021/05/28

写真a

 
SAWADA, Makoto
 
Organization
Research Institute of Environmental Medicine Division of Stress Adaptation and Protection Professor
Graduate School
Graduate School of Medicine
Title
Professor

Degree 1

  1. 理学博士 ( 1986.3   東京工業大学 ) 

Research Areas 1

  1. Others / Others  / 神経化学・神経薬理学

Current Research Project and SDGs 8

  1. 脳の機能や神経系細胞のバイオイメージング

  2. 脳の構築とミクログリアの関わり

  3. 骨髄細胞を用いた標的化再生治療

  4. understanding of neurodegenerative disorders

  5. a new strategy of brain-targeting DDS

  6. エクソソームの新規なバイオマーカーの探索

  7. LC-MSイメージング前処理装置の開発と応用

  8. 3D Mass spectrometry imaging

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Research History 18

  1. Nagoya University

    2009.4

  2. Nagoya University

    2008.11

  3. Nagoya University   Deputy Director

    2008.4 - 2010.3

  4. Nagoya University

    2006.4

  5. Nagoya University

    2006

  6. Nagoya University

    2006

  7. 名古屋大学・環境医学研究所 教授

    2005.1

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    Country:Japan

  8. 株式会社ティッシュターゲティングジャパン取締役, CSO

    2004.12

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    Country:Japan

  9. 株式会社ティッシュターゲティングジャパン、代表取締役

    2002.3 - 2004.11

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    Country:Japan

  10. 藤田保健衛生大学・総合医科学研究所・難病治療共同研究部門、教授

    2000.4 - 2004.12

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    Country:Japan

  11. 科学技術振興事業団さきがけ研究21 重点研究研究員

    2000.3 - 2002.2

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    Country:Japan

  12. 名古屋大学・生物分子応答研究センター・客員教授

    1999.4 - 2000.3

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    Country:Japan

  13. 藤田保健衛生大学・総合医科学研究所・難病治療共同研究部門、助教授

    1998.4 - 2000.3

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    Country:Japan

  14. 科学技術振興事業団さきがけ研究21 研究員

    1996.10 - 1999.9

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    Country:Japan

  15. 藤田保健衛生大学総合医科学研究所病態生化学研究部門助教授

    1996.1 - 1998.3

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    Country:Japan

  16. 藤田保健衛生大学総合医科学研究所応用細胞学 講師

    1993.6 - 1996.1

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    Country:Japan

  17. 米国国立衛生研究所ポストドクトラルフェロー

    1989.1 - 1991.1

  18. 藤田保健衛生大学総合医科学研究所応用細胞学 助手

    1986.4 - 1993.5

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    Country:Japan

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Education 3

  1. Tokyo Institute of Technology   Graduate School, Division of Integrated Science and Engineering

    1983.4 - 1986.3

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    Country: Japan

  2. Tokyo Institute of Technology   Graduate School, Division of Integrated Science and Engineering

    1981.4 - 1983.3

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    Country: Japan

  3. Tokyo Institute of Technology   Faculty of Science

    1977.4 - 1981.3

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    Country: Japan

Professional Memberships 6

  1. 脳の医学生物学研究会   幹事

    2005.4

  2. 日本バイオイメージング学会   評議員

    2003.4

  3. 日本生化学会   評議員

    2000.4

  4. 日本神経化学会   評議員

    2000.4

  5. 日本分子生物学会

  6. 日本神経科学学会

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Papers 221

  1. Secretion of signal peptides via extracellular vesicles

    Ono Kenji, Niwa Mikio, Suzuki Hiromi, Kobayashi Nahoko Bailey, Yoshida Tetsuhiko, Sawada Makoto

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 560   page: 21 - 26   2021.6

  2. Detection of Alzheimer's disease-related neuroinflammation by a PET ligand selective for glial versus vascular translocator protein. Reviewed International coauthorship

    Ji B, Ono M, Yamasaki T, Fujinaga M, Zhang MR, Seki C, Aoki I, Kito S, Sawada M, Suhara T, Sahara N, Higuchi M

    Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     page: 271678X21992457   2021.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0271678X21992457

    PubMed

  3. Peripheral benzodiazepine receptor/18 kDa translocator protein positron emission tomography imaging in a rat model of acute brain injury Reviewed International coauthorship

    Nomura Masahiko, Toyama Hiroshi, Suzuki Hiromi, Yamada Takashi, Hatano Kentaro, Wilson Alan A., Ito Kengo, Sawada Makoto

    ANNALS OF NUCLEAR MEDICINE   Vol. 35 ( 1 ) page: 8 - 16   2021.1

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    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s12149-020-01530-2

    Web of Science

    PubMed

  4. Silica Nanoparticle Induced Lung Injury in Mice: Dissecting Relative Contribution of Its Size and Surface Modification Reviewed

    Inoue M., Sakamoto K., Suzuki A., Nakahara Y., Hashimoto N., Hasegawa Y., Sawada M.

    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE   Vol. 201   2020.5

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    Web of Science

  5. Inhibition of cellular inflammatory mediator production and amelioration of learning deficit in flies by deep sea Aspergillus-derived cyclopenin Reviewed International journal

    Wang Liyan, Li Mengjie, Lin Yinzhi, Du Shuwen, Liu Zhenyu, Ju Jianhua, Suzuki Hiromi, Sawada Makoto, Umezawa Kazuo

    JOURNAL OF ANTIBIOTICS   Vol. 73 ( 9 ) page: 622 - 629   2020.9

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    DOI: 10.1038/s41429-020-0302-9

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  6. Dipeptidyl peptidase-4 inhibition prevents nonalcoholic steatohepatitis-associated liver fibrosis and tumor development in mice independently of its anti-diabetic effects Reviewed

    Kawakubo Mitsuhiro, Tanaka Miyako, Ochi Kozue, Watanabe Akiko, Saka-Tanaka Marie, Kanamori Yohei, Yoshioka Naoki, Yamashita Satoko, Goto Moritaka, Itoh Michiko, Shirakawa Ibuki, Kanai Sayaka, Suzuki Hiromi, Sawada Makoto, Ito Ayaka, Ishigami Masatoshi, Fujishiro Mitsuhiro, Arima Hiroshi, Ogawa Yoshihiro, Suganami Takayoshi

    SCIENTIFIC REPORTS   Vol. 10 ( 1 ) page: 983   2020.1

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    DOI: 10.1038/s41598-020-57935-6

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  7. Novel Oxindole-Curcumin Hybrid Compound for Antioxidative Stress and Neuroprotection Reviewed

    Hirata Yoko, Ito Yuki, Takashima Madoka, Yagyu Kazuya, Oh-hashi Kentaro, Suzuki Hiromi, Ono Kenji, Furuta Kyoji, Sawada Makoto

    ACS CHEMICAL NEUROSCIENCE   Vol. 11 ( 1 ) page: 76 - 85   2020.1

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  8. A peptide based on transmembrane domain of S1PR4 as a tumor cell growth inhibitor

    Yoshida Tetsuhiko, Tanaka Kenichi, Asakawa Shuichi, Sawada Makoto, Hasegawa Yoshinori, Nakase Ikuhiko, Kobayashi Nahoko Bailey

    MOLECULAR CANCER THERAPEUTICS   Vol. 18 ( 12 )   2019.12

  9. Anti-claudin antibodies as a concept for development of claudin-directed drugs

    Hashimoto Y, Okada Y, Shirakura K, Tachibana K, Sawada M, Yagi K, Doi T, Kondoh M

    The Journal of pharmacology and experimental therapeutics   Vol. 368 ( 2 ) page: 179 - 186   2019.2

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    DOI: 10.1124/jpet.118.252361

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  10. Selective autophagy eliminates ALS-related mutant SOD1 protein in cultured microglia

    Niida Motoko Kawaguchi, Tsukahara Fujiko, Sudou Norihiro, Yamamoto Tomoko, Sawada Makoto, Watabe Kazuhiko, Shibata Noriyuki

    BRAIN PATHOLOGY   Vol. 29   page: 94 - 95   2019.2

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  11. A peptide based on transmembrane domain of S1PR1 as a tumor cell growth inhibitor

    Kobayashi N. Bailey, Sawada M., Hasegawa Y., Yoshida T.

    EUROPEAN JOURNAL OF CANCER   Vol. 103   page: E94 - E94   2018.11

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  12. Novel oxindole derivatives prevent oxidative stress-induced cell death in mouse hippocampal HT22 cells

    Hirata Yoko, Yamada Chika, Ito Yuki, Yamamoto Shotaro, Nagase Haruna, Oh-hashi Kentaro, Kiuchi Kazutoshi, Suzuki Hiromi, Sawada Makoto, Furuta Kyoji

    NEUROPHARMACOLOGY   Vol. 135   page: 242 - 252   2018.6

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    DOI: 10.1016/j.neuropharm.2018.03.015

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  13. Inducible nitric oxide synthase during the late phase of sepsis is associated with hypothermia and immune cell migration.

    Takatani Y, Ono K, Suzuki H, Inaba M, Sawada M, Matsuda N

    Laboratory investigation; a journal of technical methods and pathology   Vol. 98 ( 5 ) page: 629 - 639   2018.5

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    DOI: 10.1038/s41374-018-0021-z

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  14. Visualization of Arc promoter-driven neuronal activity by magnetic resonance imaging

    Wu Qi, Ono Kenji, Suzuki Hiromi, Eguchi Megumi, Yamaguchi Shun, Sawada Makoto

    NEUROSCIENCE LETTERS   Vol. 666   page: 92-97   2018.2

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    DOI: 10.1016/j.neulet.2017.12.041

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  15. Extracellular Lactate Dehydrogenase A Release From Damaged Neurons Drives Central Nervous System Angiogenesis

    Lin Hsiaoyun, Muramatsu Rieko, Maedera Noriko, Tsunematsu Hiroto, Hamaguchi Machika, Koyama Yoshihisa, Kuroda Mariko, Ono Kenji, Sawada Makoto, Yamashita Toshihide

    EBIOMEDICINE   Vol. 27   page: 71-85   2018.1

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    DOI: 10.1016/j.ebiom.2017.10.033

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  16. LC-MS/MS imaging with thermal film-based laser microdissection

    Oya Michiko, Suzuki Hiromi, Anas Andrea Roxanne J., Oishi Koichi, Ono Kenji, Yamaguchi Shun, Eguchi Megumi, Sawada Makoto

    ANALYTICAL AND BIOANALYTICAL CHEMISTRY   Vol. 410 ( 2 ) page: 491-499   2018.1

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    DOI: 10.1007/s00216-017-0739-2

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  17. Optogenetic control of cell differentiation in channelrhodopsin-2-expressing OS3, a bipotential glial progenitor cell line

    Ono K, Suzuki H, Yamamoto R, Sahashi H, Takido Y, Sawada M

    Neurochemistry International   Vol. 104   page: 49-63   2017.3

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    DOI: 10.1016/j.neuint.2016.12.022.

  18. The novel monoclonal antibody 9F5 reveals expression of a fragment of GPNMB/osteoactivin processed by furin-like protease(s) in a subpopulation of microglia in neonatal rat brain

    Kawahara K, Hirata H, Ohbuchi K, Nishi K, Maeda A, Kuniyasu A, Yamada D, Maeda T, Tsuji A, Sawada M, Nakayama H

      Vol. 64 ( 11 ) page: 1938-1961   2016.11

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    DOI: 10.1002/glia.23034

  19. Soluble Siglec-9 suppresses arthritis in a collagen-induced arthritis mouse model and inhibits M1 activation of RAW264.7 macrophages

    Matsumoto T,Takahashi N, Kojima T, Yoshioka Y, Ishikawa J, Furukawa K, Ono K, Sawada M, Ishiguro N,Yamamoto A

    Arthritis research & therapy   Vol. 18 ( 1 ) page: 133   2016.6

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    DOI: 10.1186/s13075-016-1035-9.

  20. Nanoparticles speckled by ready-to-conjugate lanthanide complexes for multimodal imaging

    Biju V, Hamada M, Ono K, Sugino S, Ohnishi T, Shibu ES, Yamamura S, Sawada M, Nakanishi S, Shigeri Y, Wakida SI.

    Nanoscale   Vol. 7 ( 36 ) page: 14829-14837   2015.9

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    DOI: 10.1039/c5nr00959f

  21. Insular neural system controls decision-making in healthy and methamphetamine-treated rats

    Mizoguchi H, Katahira K, Inutsuka A, Fukumoto K, Nakamura A, Wang T, Nagai T, Sato J, Sawada M, Ohira H, Yamanaka A, Yamada K

    Proceedings of the National Academy of Sciences of the United States of America   Vol. 112 ( 29 ) page: E3930-3939   2015.7

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    DOI: 10.1073/pnas.1418014112

  22. Rapid trimming of cell surface polySia by exovesicular sialidase triggers release of preexisting surface neurotrophin

    Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C.

    The Journal of Biological Chemistry   Vol. 290 ( 21 ) page: 13202-13214   2015.5

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    DOI: 10.1074/jbc.M115.638759

  23. Radiosynthesis and in vivo evaluation of two imidazopyridineacetamides, [11C]CB184 and [ 11C]CB190, as a PET tracer for 18 kDa translocator protein: direct comparison with [ 11C](R)-PK11195.

    Hatano K, Sekimata K, Yamada T, Abe J, Ito K, Ogawa M, Magata Y, Toyohara J, Ishiwata K, Biggio G, Serra M, Laquintana V, Denora N, Latrofa A, Trapani G, Liso G, Suzuki H, Sawada M, Nomura M, Toyama H.

    Ann Nucl Med   Vol. 29 ( 4 ) page: 325-335   2015.5

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    DOI: 10.1007/s12149-015-0948-8

  24. Nanoparticles speckled by ready-to-conjugate lanthanide complexes for multimodal imaging

    Biju V, Hamada M, Ono K, Sugino S, Ohnishi T, Shibu ES, Yamamura S, Sawada M, Nakanishi S, Shigeri Y, Wakida SI.

    Nanoscale     page: in press   2015

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  25. Insular neural system controls decision-making in healthy and methamphetamine-treated rats

    Mizoguchi H, Katahira K, Inutsuka A, Fukumoto K, Nakamura A, Wang T, Nagai T, Sato J, Sawada M, Ohira H, Yamanaka A, Yamada K

    Proc. Natl. Acad. Sci. U.S.A     page: in press   2015

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  26. Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells

    YUKAWA H, NAKAGAWA S, YOSHIZUMI Y, WATANABE M, SAITO H, MIYAMOTO Y, NOGUCHI H, OISHI K, ONO K, SAWADA M, KATO I, ONOSHIMA D, OBAYASHI M, HAYASHI Y, KAJI N, ISHIKAWA T, HAYASHI S, BABA Y

    PLoS One   Vol. 9 ( 11 ) page: e110142   2014.11

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    DOI: 10.1371/journal.pone.0110142

  27. Protective effect of INI-0602, a gap junction inhibitor, on dopaminergic neurodegeneration of mice with unilateral 6-hydroxydopamine injection

    SUZUKI Hiromi, ONO Kenji, SAWADA Makoto

    Journal of nueral Transmission   Vol. 121 ( 11 ) page: 1349-1355   2014.11

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  28. Glutamate release from astrocyte cell-line GL261 via alterations in the intracellular ion environment Reviewed

    ONO K, SUZUKI H, HIGA M, TABATA K, SAWADA M

    Journal of Neural Transmission   Vol. 121 ( 3 ) page: 245-257   2014.3

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    DOI: 10.1007/s00702-013-1096-8

  29. Increases in intracellular pH facilitate endocytosis and decrease availability of voltage-gated proton channels in osteoclasts and microglia Reviewed

    Sakai H, Li G, Hino Y, Moriura Y, Kawawaki J, Sawada M, Kuno M.

    The Journal of Physiology   Vol. 591 ( Pt23 ) page: 5851-5866   2013.12

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    DOI: 10.1113/jphysiol.2013.263558

  30. The effect of lipid emulsion on intracellular bupivacaine as a mechanism of lipid resuscitation: an electrophysiological study using voltage-gated proton channels Reviewed

    HORI Kotaro, MATSUURA Tadashi, MORI Takashi, KUNO Miyuki, SAWADA Makoto, NISHIKAWA Kiyonobu

    Anesthesia and analgesia   Vol. 117 ( 6 ) page: 1293-1301   2013.12

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    DOI: 10.1213/ANE.0000000000000011

  31. Photouncaging nanoparticles for MRI and fluorescence imaging in vitro and in vivo Reviewed

    SHIBU Edakkattuparambil Sidharth, ONO Kenji, SUGINO Sakiko, NISHIOKA Ayami, YASUDA Akikazu, SHIGERI Yasushi, WAKIDA Shin-ichi, SAWADA Makoto, BIJU Vasudevanpillai

    American Chemical Society nano   Vol. 7 ( 11 ) page: 9851-9859   2013.11

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  32. Singlet-Oxygen-Sensitizing Near-Infrared-Fluorescent Multimodal Nanoparticles Reviewed

    Shibu ES, Sugino S, Ono K, Saito H, Nishioka A, Yamamura S, Sawada M, Nosaka Y, Biju V

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   Vol. 52 ( 40 ) page: 10559-10563   2013.9

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    DOI: 10.1002/anie.201304264

  33. In vivo imaging of transplanted islets labeled with a novel cationic nanoparticle Reviewed

    OISHI Koichi, MIYAMOTO Yoshitaka, SAITO Hiroaki, MURASE Katsutoshi, ONO Kenji, SAWADA Makoto, WATANABE Masami, NOGUCHI Yasufumi, FUJIWARA Toshiyoshi, HAYASHI Shuji, NOGUCHI Hirofumi.

    PLoS one   Vol. 8 ( 2 ) page: e57046   2013.2

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  34. Novel positive-charged nanoparticles for efficient magnetic resonance imaging of islet transplantation.

    OISHI Kouichi, NOGUCHI Hirofumi, SAITO Hiroaki,YUKAWA H, MIYAMOTO Yoshitaka, ONO Kenji, MURASE Katsutoshi, SAWADA Makoto, HAYASHI Shuji

    Cell Medicine   Vol. 3 ( 1-3 ) page: 43-49   2012.10

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  35. Hemokinin-1 gene expression is upregulated in microglia activated by lipopolysaccharide through NFkB and p38 MAPK signaling pathways

    SAKAI Atsushi, TAKASU Kumiko, SAWADA Makoto, SUZUKI Hidenori

    PLoS one   Vol. 7 ( 2 ) page: e32268   2012.2

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  36. Inhibition of voltage-gated proton channels by local anaesthetics in GMI-R1 rat microglia Reviewed

    Matsuura T, Mori T, Hasaka M, Kuno M, Kawawaki J, Nishikawa K, Narahashi T, Sawada M, Asada A

    The Journal of Physiology   Vol. 590 ( Pt4 ) page: 827-844   2012.2

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  37. New fluorescent probes targeting the mitochondrial-located translocator protein 18 kDa (TSPO) as activated microglia imaging agents. Reviewed

    DENORA N, LAQUINTANA V, TRAPANI A, SUZUKI H, SAWADA M, TRAPANI G

    Pharmaceutical Research   Vol. 28 ( 11 ) page: 2820-2382   2011.11

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  38. Alzheimer's disease and microglia

    HIGUCHI M, JI B, MAEDA J, MARUYAMA M, ONO M, SAWADA M, SUHARA T

    Rinsho Shinkeigaku   Vol. 51 ( 11 ) page: 1031   2011.11

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  39. Effects of a Brain-Engraftable Microglial Cell Line Expressing Anti-Prion scFv Antibodies on Survival Times of Mice Infected with Scrapie Prions Reviewed

    FUJITA K, YAMAGUCHI Y, MORI T, MURAMATSU N, MIYAMOTO T, YANO M, MIYATA H, OOTSUYAMA A, SAWADA M, MATSUDA H, KAJI R, SAKAGUCHI S

    Cellular and Molecular neurobioloy   Vol. 31 ( 7 ) page: 999-1008   2011.10

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  40. Matrix Metalloproteinase-9 Contributes to Kindled Seizure Development in Pentylenetetrazole-Treated Mice by Converting Pro-BDNF to Mature BDNF in the Hippocampus. Reviewed

    MIZOGUCHI H, NAKADE J, TACHIBANA M, IBI D, SOMEYA E, KOIKE H, KAMEI H, NABESHIMA T, ITOHARA S, TAKUMA K, SAWADA M, SATO J, YAMADA K

    Journal of Neuroscience   Vol. 31 ( 36 ) page: 12963-12971   2011.9

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  41. 4-hydroxy-2-nonenal upregulates and phosphorylates cytosolic phospholipase A (2) in cultured Ra2 microglial cells via MAPK pathways Reviewed

    SHIBATA N, KATO Y, INOSE Y, HIROI A, YAMAMOTO T, MORIKAWA S, SAWADA M, KOBAYASHI M

    Neuropathology   Vol. 31 ( 2 ) page: 122-128   2011.4

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  42. Time-dependent changes in proinflammatory and neurotrophic responses of microglia and astrocytes in a rat model of osmotic demyelination syndrome. Reviewed

    IWAMA S, SUGIMURA Y, SUZUKI H, SUZUKI H, MURASE T, OZAKI N, NAGASAKI H, ARIMA H, MURATA Y, SAWADA M, OISO Y

    Glia   Vol. 59 ( 3 ) page: 452-462   2011.3

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  43. Effects of a Brain-Engraftable Microglial Cell Line Expressing Anti-Prion scFv Antibodies on Survival Times of Mice Infected with Scrapie Prions Reviewed

    FUJITA K, YAMAGUCHI Y, MORI T, MURAMATSU N, MIYAMOTO T, YANO M, MIYATA H, OOTSUYAMA A, SAWADA M, MATSUDA H, KAJI R, SAKAGUCHI S

    Cellular and Molecular neurobioloy     page: in press   2011

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  44. Electrosprayed Synthesis of Red-Blood-Cell-Like Particles with Dual Modality for Magnetic Resonance and Fluorescence Imaging. Reviewed

    HAYASHI K, ONO K, SUZUKI H, SAWADA M, MORIYA M, SAKAMOTO W, YOGO T

    Small   Vol. 6 ( 21 ) page: 2384-2391   2010.11

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  45. High-frequency, magnetic-field-responsive drug release from magnetic nanoparticle/organic hybrid based on hyperthermic effect.

    HAYASHI K, ONO K, SUZUKI H, SAWADA M, MORIYA M, SAKAMOTO W, YOGO T

    ACS applied materials & interfaces   Vol. 2 ( 7 ) page: 1903-1911   2010.7

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  46. One-pot biofunctionalization of magnetic nanoparticles via thiol-ene click reaction for magnetic hyperthermia and magnetic resonance imaging Reviewed

    HAYASHI K, ONO K, SUZUKI H, SAWADA M, MORIYA M, SAKAMOTO W, YOGO T

    Chemistry of Materials   Vol. 22 ( 12 ) page: 3768-3772   2010.6

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  47. Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells. Reviewed

    Yukawa H, Kagami Y, Watanabe M, Oishi K, Miyamoto Y, Okamoto Y, Tokeshi M, Kaji N, Noguchi H, Ono K, Sawada M, Baba Y, Hamajima N, Hayashi S.

    Biomaterials   Vol. 31 ( 14 ) page: 4094-4103   2010.5

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  48. Two activated stages of microglia and PET imaging of peripheral benzodiazepine receptors with [(11)C]PK11195 in rats Reviewed

    ITO Fumitaka, TOYAMA Hiroshi, KUDO Gen, SUZUKI Hiromi, HATANO Kentaro, ICHISE Masanori, KATADA Kazujhiro, ITO Kengo, SAWADA Makoto

    Annals of Nuclear Medecine   Vol. 24 ( 3 ) page: 163-169   2010.4

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  49. Delayed neural damage is induced by iNOS-expressing microglia in a brain injury model. Reviewed

    ONO K, SUZUKI H, SAWADA M

    Neuroscience Letters   Vol. 473 ( 2 ) page: 146-150   2010.4

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  50. Neuroprotective and Neurotoxic Phenotypes of Activated Microglia in Neonatal Mice with Respective MPTP- and Ethanol-Induced Brain Injury. Reviewed

    Sawada H, Suzuki H, Nagatsu T, Sawada M.

    Neuro-degenerative Diseases   Vol. 7 ( 1-3 ) page: 64-67   2010.2

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  51. 4-hydroxy-2-nonenal upregulates and phosphorylates cytosolic phospholipase A (2) in cultured Ra2 microglial cells via MAPK pathways Reviewed

    SHIBATA N, KATO Y, INOSE Y, HIROI A, YAMAMOTO T, MORIKAWA S, SAWADA M, KOBAYASHI M

    Neuropathology   Vol. in press   2010

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  52. Minocycline prevents osmotic demyelination syndrome by inhibiting the activation of microglia. Reviewed

    SUZUKI H, SUGIMURA Y, IWAMA S, SUZUKI H, OZAKI N, NAGASAKI H, ARIMA H, SAWADA M, OISO Y.

    Journal of the American Society of Nephrology   Vol. 21 ( 12 ) page: 2090-2098   2010

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  53. *Ferritin reporter used for gene expression imaging by magnetic resonance. Reviewed

    ONO Kenji, FUMA Kazuya, TABATA Kaori, SAWADA Makoto

    Biochemical and Biophysical Research Communications   Vol. 388 ( 3 ) page: 589-594   2009.10

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  54. Temperature dependence of proton permeation through a voltage-gated proton channel Reviewed

    KUNO Miyuki, ANDO Hiroyuki , MORIHATA Hirokazu, SAKAI Hiromu, MORI Hiroyuki, SAWADA Makoto, OIKI Shigetoshi

    The Journal of General Physiology   Vol. 134 ( 3 ) page: 191-205   2009.9

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  55. Interleukin-6 induces prostaglandin E2 synthesis in mouse astrocytes Reviewed

    CHIKUMA T, YOSHIMOTO T, OHBA M, SAWADA M, KATO T, SAKAMOTO T, HIYAMA Y, HOJO H

    Journal of Molecilar Neuroscience   Vol. 39 ( 1-2 ) page: 175-184   2009.9

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  56. Marked induction of inducible nitric oxide synthase and tumor necrosis factor-alpha in rat CD40(+) microglia by comparison to CD40(-) microglia.

    KAWAHARA K, YOSHIDA A, KOGA K, YOKOO S,KUNIYASU A, GOTOH T, SAWADA M, NAKAYAMA H

    Journal of Neuroimmunology   Vol. 208 ( 1-2 ) page: 70-79   2009.3

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  57. Transforming growth factor-beta 1 upregulates keratan sulfate and chondroitin sulfate biosynthesis in microglias after brain injury Reviewed

    YIN Jiarong, SAKAMOTO Kazuma, ZHANG Haoqian, ITO Zenya, IMAGAMA Shiro, KISHIDA Satoshi, NATORI Takamitsu, SAWADA Makoto, MATSUYAMA Yukihiro, KADOMATSU Kenji

    Brain Research   Vol. 1263   page: 10-22   2009.3

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  58. Neuroprotective and toxic changes in microglia in neurodegenerative disease Reviewed

    SAWADA Makoto

    Parkinsonism & Related Disorders   Vol. 15   page: S39-S41   2009.1

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  59. L-dopa therapy for Parkinson's disease: Past, present, and future Reviewed

    NAGATSU T, SAWADA M

    Parkinsonism & Related Disorders   Vol. 15   page: S3-S8   2009.1

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  60. A link between stress and depression: Shifts in the balance between the kynurenine and serotonin pathways of tryptophan metabolism and the etiology and pathophysiology of depression Reviewed

    MIURA H, OZAKI N, SAWADA M, ISOBE K, OHTA T, NAGATSU T

    Stress-The International Journal on the Biology of Stress)   Vol. 12 ( 5 ) page: 198-209   2009

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  61. Increase in hemokinin-1 mRNA in the spinal cord during the early phase of a neuropathic pain state Reviewed

    MATSUMURA T, SAKAI A, NAGANO M, SAWADA M, SUZUKI H, UMINO M, SUZUKI H

    Brit J Pharmacol   Vol. 155 ( 5 ) page: 767-774   2008.11

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  62. Blockade of microglial glutamate release protects against ischemic brain injury Reviewed

    TAKEUCHI H,SHIJIE J, SUZUKI H, DOI Y, J. Liang, KAWANOKUCHI J, MIZUNO T, SAWADA M, SUZUMUTRA A

    Exp Neurol   Vol. 214 ( 1 ) page: 144-146   2008.11

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  63. *Imaging of Peripheral Benzodiazepine Receptor Expression as Biomarkers of Detrimental versus Beneficial Glial Responses in Mouse Models of Alzheimer's and Other CNS Pathologies. Reviewed

    Ji B, MAEDA J, SAWADA M, ONO M, OKAUCHI T, INAJI M, Zhang MR, SUZUKI K, ANDO K, Staufenbiel M, Trojanowski JQ, Lee VM, HIGUCHI M, SUHARA T

    J Neurosci   Vol. 28 ( 47 ) page: 12255-12267   2008.11

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  64. Interleukin-4­induced Selective Clearance of Oligomeric b-Amyloid peptide by Rat Primary Type-2 Microglia Reviewed

    SHIMIZU E, KAWAHARA K , KAJIZONO M, SAWADA M, NAKAYAMA H

    J Immunol   Vol. 181 ( 9 ) page: 6503-6513   2008.11

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  65. In vivo imaging of microglial activation using a peripheral benzodiazepine receptor ligand: [(11)C]PK-11195 and animal PET following ethanol injury in rat striatum Reviewed

    TOYAMA H, HATANO K, SUZUKI H, ICHISE M, MOMOSAKI S, KUDO G, ITO F, KATO T, YAMAGUCHI H, KATADA K, SAWADA M, ITO K

    Ann Nucl Med   Vol. 22 ( 5 ) page: 417-424   2008.6

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  66. A link between stress and depression: shifts in the balance between the kynurenine and serotonin pathways of tryptophan metabolism and the etiology and pathophysiology of depression. Reviewed

    MIURA H, OZAKI N, SAWADA M, ISOBE K, OHTA T, NAGATSU T

    Stress-The International Journal on the Biology of Stress   Vol. 11 ( 3 ) page: 198-209   2008.5

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  67. Effects of aging on neuroprotective and neurotoxic properties of microglia in neurodegenerative diseases. Reviewed

    SAWADA Makoto, SAWADA Hirohide, NAGATSU Toshiharu

    Neurodegener Dis   Vol. 5 ( 3-4 ) page: 254-256   2008.3

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  68. Stimulus dependent regulation of the phagocyte NADPH oxidase by a VAV1, rac 1, and PAK1 signaling axis. Reviewed

    ROEPSTORFF Kirstine, RASMUSSEN Izabela, SAWADA Makoto, CUDRE-MAROUX Cristophe, SALOMON Patrick, BOKOCH Gary, VAN DEURS Bo, VIHARDT Frederik

    J Biol Chem   Vol. 283 ( 12 ) page: 7983-7993   2008.3

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  69. Early and late activation of the voltage-gated proton channel during lactic acidosis through pH-dependent and -independent mechanisms Reviewed

    MORIHATA Hirokazu, KAWAWAKI Junko, OKINA Masako, SAKAI Hiromu, NOTOMI Takuya, SAWADA Makoto, KUNO Miyuki

    Pflugers Arch   Vol. 455 ( 5 ) page: 829-838   2008.2

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  70. N-Benzyl-2-(6,8-dichloro-2-(-(4-chlorophenyl)imidazo[1,2-alpyridin-3-yl)-N-(6-(7-nitrobenzo[c][1,2,5]oxadiazol-4-ylamino) hexyl)acetamide as a New Fluorescent Probe for Peripheral Benzodiazepine Receptor and Microglial Cell Visualization. Reviewed

    LAQUINTANA Valentino, DENORA Nunzio, LOPEDOTA Angela, SUZUKI Hiromi, SAWADA Makoto, SERRA Mariangela, BIGGIO Giovanni, LATROFA Andrea, TRAPANI Giuseppe, LISO Gaetano

    Bioconjug Chem   Vol. 18 ( 5 ) page: 1397-1407   2007.9

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  71. Antidepressants inhibit interferon-gamma-induced microglial production of IL-6 and nitric oxide Reviewed

    Hashioka, S., Klegeris, A., Monji, A., Kato, T., Sawada, M., McGeer, P.L. and Kanba, S.

      Vol. 206 ( 0 ) page: 33-42   2007.7

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    Circumstantial evidence has suggested that activated microglia may be associated with the pathogenesis of depression. Pro-inflammatory cytokines may also be involved. Therefore, we examined the effects of various types of antidepressants, as well as the mood-stabilizer lithium chloride, on interferon-gamma (IFN-gamma)-induced microglial production of the pro-inflammatory mediators interleukin-6 (IL-6) and nitric oxide (NO). Treatment of the murine microglial 6-3 cells with 100 U/ml of IFN-gamma resulted in an eightfold increase in IL-6 and a tenfold increase in NO into the culture medium. Pretreatment with the selective serotonin reuptake inhibitor fluvoxamine, the relatively selective noradrenaline reuptake inhibitor reboxetine, or the non-selective monoaminergic reuptake inhibitor imipramine, significantly inhibited IL-6 and NO production in a dose-dependent manner. These inhibitions were reversed significantly by SQ 22536, a cyclic adenosine monophosphate (cAMP) inhibitor, and, except for reboxetine, by the protein kinase A (PKA) inhibitor Rp-adenosine3',5'-cyclic monophosphorothioate triethylammonium salt (Rp-3',5'-cAMPS). Lithium chloride, which is believed to act by inhibiting the calcium-dependent release of noradrenaline, had a different spectrum of action on microglial 6-3 cells. It enhanced IFN-gamma-stimulated IL-6 production and inhibited NO production. The inhibitory effect of lithium chloride was not reversed by either SQ 22536 or Rp-3',5'-cAMPS. These results suggest that antidepressants have inhibitory effects on IFN-gamma-activated microglia and these effects are, at least partially, mediated by the cAMP-dependent PKA pathway. On the other hand, the mood stabilizer and anti-manic agent lithium chloride has mixed effects on IFN-gamma-induced microglial activation.

  72. Activated microglia affect the nigro-striatal dopamine neurons differently in neonatal and aged mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine

    Sawada, H., Hishida, R., Hirata, Y., Ono, K., Suzuki, H., Muramatsu, S., Nakano, I., Nagatsu, T. and Sawada, M.

    J Neurosci Res   Vol. 85 ( 8 ) page: 1752-1761   2007.6

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    Microglia play an important role in the inflammatory process that occurs in Parkinson's disease (PD). Activated microglia produce cytokines and neurotrophins and may have neurotoxic or neurotrophic effects. Because microglia are most proliferative and easily activated during the neonatal period, we examined the effects of neonatal microglia activated with lipopolysaccharide (LPS) on the nigro-striatal dopamine neurons in mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), in comparison with activated microglia from the aged mice. By MPTP administration to neonatal mice, the number of dopamine neurons in the substantia nigra (SN) was decreased significantly, whereas that in the mice treated with LPS and MPTP was recovered to normal, along with significant microglial activation. Tyrosine hydroxylase (TH) activity, the levels of dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC), and the levels of pro-inflammatory cytokines IL-1beta and IL-6 in the midbrain were elevated in the neonates treated with LPS and MPTP. On the contrary, although the number of dopamine neurons in the 60-week-old mice treated with MPTP was also decreased significantly, the microglial activation by LPS treatment caused a further decrease in their number. These results suggest that the activated microglia in neonatal mice are different from those in aged mice, with the former having neurotrophic potential toward the dopamine neurons in the SN, in contrast to the neurotoxic effect of the latter.

  73. Induction of matrix metalloproteinases (MMP3, MMP12 and MMP13) expression in the microglia by amyloid-beta stimulation via the PI3K/Akt pathway Reviewed

    Ito, S., Kimura, K., Haneda, M., Ishida, Y., Sawada, M. and Isobe, K.

      Vol. 42 ( 6 ) page: 532-537   2007.6

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    Alzheimer's disease is characterized by the presence of senile plaques in the brain composed primarily of amyloid-beta peptide. Microglia have been reported to surround these Abeta plaques, which have opposite roles, provoking a microglia-mediated inflammatory response that contributes to neuronal cell loss or the removal of Abeta and damaged neurons. We herein analyzed the process of expression of Matrix metalloproteinases induced by Abeta stimulation. We found that Abeta1-42 induces a high level of MMP3, MMP12 and MMP13 in the microglia. The signal transduction pathway for the expression of these MMPs mRNA induced by Abeta1-42 depends on PI3K/Akt.

  74. The effects of general anesthetics on P2X7 and P2Y receptors in a rat microglial cell line Reviewed

    Nakanishi, M., Mori, T., Nishikawa, K., Sawada, M., Kuno, M. and Asada, A.

    Anesth Analg   Vol. 101 ( 5 ) page: 1136-1144   2007.5

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    BACKGROUND: Microglial cells play important roles in coordinating the inflammatory brain responses to hypoxia and trauma. Ionotropic P2X receptors and metabotropic P2Y receptors (P2YRs) expressed in microglia can be activated by extracellular adenosine triphosphate (ATP) derived from damaged cells or astrocytes, and participate in the signaling pathways evoked in brain insult. Although several inhaled and IV anesthetics produce neuroprotective effects through neuronal mechanisms, little is known about how general anesthetics modulate microglial responses in the pathological state. We examined the effects of various general anesthetics on purinergic responses in a rat microglial cell line. METHODS: Currents were consistently activated by applications of ATP via a U-tube system under the whole-cell configuration. ATP-induced nondesensitizing currents observed after several applications of ATP exhibited characteristics of P2X7 receptors. The P2YRs-mediated mobilization of intracellular Ca2+ was measured using a Ca2+-sensitive fluorescent dye (fura-2). RESULTS: Inhaled anesthetics (sevoflurane, isoflurane, and halothane) at doses three times as high as minimum alveolar concentrations had no effect on the P2X7Rs-mediated currents. IV anesthetics (ketamine, propofol, and thiopental) enhanced the P2X7Rs-mediated currents reversibly. The potencies for activation of P2X7Rs were not correlated with the octanol/buffer partition coefficients. Thiopental, at low concentrations, slightly inhibited the P2X7Rs-mediated currents, suggesting its dual actions on P2X7Rs. The P2YRs-mediated mobilization of intracellular Ca2+ was not affected by any of the general anesthetics tested. CONCLUSIONS: Our results suggest that IV anesthetics, particularly thiopental and propofol, may modulate microglial functions through P2X7Rs in pathological conditions.

  75. Identification of ligands binding specifically to inflammatory intestinal mucosa using phage display Reviewed

    Takagi, T., Arisawa, T., Yamamoto, K., Hirata, I., Nakano, H. and Sawada, M

    Clin Exp Pharmacol Physiol   Vol. 34 ( 4 ) page: 286-289   2007.4

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    1. Even though current treatments for inflammatory bowel disease are effective, adverse reactions remain a problem. With the intention of developing a new drug delivery system, we attempted to identify molecules that are selectively adsorbed to inflamed bowel. 2. The PhD-C7C phage display peptide library was used for biopanning against mouse isolated bowel, either untreated (control) or with inflammation caused by ischaemia-reperfusion injury. One hundred clones were selected from among those obtained by two biopanning procedures and the amino acid sequences of these clones were identified by determination of the base sequences. 3. Then, 20 clones were selected by an alignment process, after which the three clones with the highest affinity for inflammatory bowel were identified. One of these three clones had significantly higher affinity for inflammatory bowel than for normal bowel. 4. In conclusion, biopanning against isolated bowel samples identified an amino acid sequence (SQSHPRH) with a specific high affinity for inflammatory bowel.

  76. Phosphatidylserine and phosphatidylcholine-containing liposomes inhibit amyloid beta and interferon-gamma-induced microglial activation Reviewed

    Hashioka, S., Han, Y.H., Fujii, S., Kato, T., Monji, A., Utsumi, H., Sawada, M., Nakanishi, H. and Kanba, S.

    Free Radic Biol Med   Vol. 42 ( 7 ) page: 945-954   2007.4

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    There is increasing evidence that microglial activation is one of the major pathogenic factors for Alzheimer's disease (AD) and the inhibition of the inflammatory activation of the microglia thus appears to be neuroprotective and a potentially useful treatment for AD. Phospholipids such as phosphatidylserine (PS) and phosphatidylcholine (PC) have been reported to modulate the immune function of phagocytes. In addition, PS has been reported to be a nootropics that can be used as nonprescription memory or cognitive enhancers. We therefore evaluated the effects of liposomes, which comprise both PS and PC (PS/PC liposomes), on the microglial production of tumor necrosis factor-alpha (TNF-alpha), nitric oxide (NO), and superoxide (*O(2)-) induced by amyloid beta (Abeta) and interferon-gamma (IFN-gamma). Pretreatment of microglia with PS/PC liposomes considerably inhibited the TNF-alpha, NO and *O(2)- production induced by Abeta/IFN-gamma. These results suggest that PS/PC liposomes have both neuroprotective and antioxidative properties through the inhibition of microglial activation, thus supporting the nootropic and antidementia effect of PS.

  77. *Neuroprotective effect of exogenous microglia in global brain ischemia Reviewed

    IMAI Fumihiro, SUZUKI Hiromi, ODA Jumpei, NINOMIYA Takashi, ONO Kenji, SANO Hirotoshi, SAWADA Makoto

    Journal of Cerebral Blood Flow & Metabolism   Vol. 27 ( 3 ) page: 488-500   2007.3

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    Exogenous microglia pass through the blood-brain barrier and migrate to ischemic hippocampal lesions when injected into the circulation. We investigated the effect of exogenous microglia on ischemic CA1 pyramidal neurons. Microglia were isolated from neonatal mixed brain cultures, labeled with the fluorescent dye PKH26, and injected into the subclavian artery of Mongolian gerbils subjected to ischemia reperfusion neuronal injury. PKH26-labeled microglia migrated to the ischemic hippocampal lesion, resulting in increased numbers of surviving neurons compared with control animals, even when injected 24 h after ischemia. Interferon-gamma stimulation of isolated microglia enhanced the neuroprotective effect. Administration of exogenous microglia resulted in normal performance in a passive avoidance-learning task. Additionally, administration of exogenous microglia increased the expression of brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor in the ischemic hippocampus, and thus might have induced neurotrophin-dependent protective activity in damaged neurons. Peripherally injected microglia exhibited a specific affinity for ischemic brain lesions, and protected against ischemic neuronal injury in vivo. It is possible that administration of exogenous microglia can be developed as a potential candidate therapy for central nervous system repair after transitory global ischemia.

  78. Targeting and imaging of brain-specific cell migration Invited

      Vol. 65 ( 2 ) page: 213-218   2007.2

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    We found that microglia and a small part of bone marrow derived progenitor cells had a specific affinity and migrating activity to the brain. The migration seemed to occur without breakage of blood brain barrier. To demonstrate the specific migration by in vivo micro imaging, we assembled a high-speed laser confocal microscope and applied it to investigate the flowing cells injected in blood stream as a funduscopy. For in vivo macro imaging we successfully observed the cell migration by MRI with an iron oxide particle cell labeling technique. We also tried to investigate such cell migration by in vivo fluorescent imaging system and detected the cells labeled with Qdot800.

  79. Phospholipids modulate superoxide and nitric oxide production by lipopolysaccharide and phorbol 12-myristate-13-acetate-activated microglia. Reviewed

    HASHIOKA Sadayuki, HAN Youn-Hee, FUJII Shunsuke, KATO Takahiro, MONJI Akira, UTSUMI Hideo, SAWADA Makoto, NAKANISHI Hiroshi, KANBA Shigenobu

    Neurochemistry International   Vol. 50 ( 3 ) page: 499-506   2007.2

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    Microglial activation and inflammatory processes have been implicated in the pathogenesis of a number of neurodegenerative disorders. Recently, peroxynitrite (ONOO(-)), the reaction product of superoxide (O(2)(-)) and nitric oxide (NO) both of which can be generated by activated microglia, has been demonstrated to act as a major mediator in the neurotoxicity induced by activated microglia. On the other hand, phospholipids such as phosphatidylserine (PS) and phosphatidylcholine (PC) have been reported to modulate the immune function of phagocytes. We therefore evaluated the effects of liposomes which comprise both PS and PC (PS/PC liposomes) or PC only (PC liposomes) regarding the production of both O(2)(-) and NO by lipopolysaccharide (LPS)/phorbol 12-myristate-13-acetate (PMA)-activated microglia using electron spin resonance (ESR) spin trap technique with a DEPMPO and Griess reaction, respectively. Pretreatment with PS/PC liposomes or PC liposomes considerably inhibited the signal intensity of O(2)(-) adduct associated with LPS/PMA-activated microglia in a dose-dependent manner. In addition, pretreatment with PS/PC liposomes also significantly reduced LPS/PMA-induced microglial NO production. In contrast, pretreatment with PC liposomes had no effect on the NO production. These results indicate that PS/PC liposomes can inhibit the microglial production of both NO and O(2)(-), and thus presumably prevent a subsequent formation of ONOO(-). Therefore, PS/PC liposomes appear to have both neuroprotective and anti-oxidative properties through the inhibition of microglial activation.

  80. Biochemistry of postmortem brains in Parkinson's disease: historical overview and future prospects Reviewed

    NAGATSU Toshiharu, SAWADA Makoto

    J Neural Transm Suppl.   Vol. 72   page: 113-120   2007

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  81. Amyloid-beta peptides induce several chemokine mRNA expressions in the primary microglia and Ra2 cell line via PI3K/Akt and/or ERK pathway. Reviewed

    ITO Sachiko, SAWADA Makoto, HANEDA Masataka, ISHIDA Yoshi, ISOBE Ken-ichi

    Neuroscience Research   Vol. 56 ( 3 ) page: 294-299   2006.11

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    primarily of amyloid-beta peptide (Abeta) in the brain. Microglia have been reported to surround these Abeta plaques, which have opposite roles, provoking a microglia-mediated inflammatory response that contributes to neuronal cell loss or the removal of Abeta and damaged neurons. To perform these tasks microglia migrate to the sites of Abeta secretion. We herein analyzed the process of chemokine expression induced by Abeta stimulation in primary murine microglia and Ra2 microglial cell line. We found that Abeta1-42 induced the expressions of CCL7, CCL2, CCL3, CCL4 and CXCL2 in the microglia. The signal transduction pathway for the expression of CCL2 and CCL7 mRNA induced by Abeta1-42 was found to depend on phosphatidylinositol 3-kinase (PI3K)/Akt and extracellular signal-regulated kinase (ERK), whereas the pathway for CCL4 depended only on PI3K/Akt. These inflammatory chemokine expressions by Abeta stimulation emphasize the contribution of neuroinflammatory mechanisms to the pathogenesis of AD.

  82. Role of cytokines in inflammatory process in Parkinson's disease Invited Reviewed

    SAWADA Makoto, IMAMURA Kazuhiro, NAGATSU Toshiharu

    Journal of Neural Transmission Supplement   Vol. 70   page: 373-381   2006.11

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  83. Cell cycle-dependent regulation of kainate-induced inward currents in microglia. Reviewed

    Yamada Jun, Sawada Makoto, Nakanishi Hiroshi

    Biochemical and Biophysical Research Communications   Vol. 349 ( 3 ) page: 913-919   2006.10

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    Microglia are reported to have alpha-amino-hydroxy-5-methyl-isoxazole-4-propionate/kainate (KA) types. However, only small population of primary cultured rat microglia (approximately 20%) responded to KA. In the present study, we have attempted to elucidate the regulatory mechanism of responsiveness to KA in GMIR1 rat microglial cell line. When the GMIR1 cells were plated at a low density in the presence of granulocyte macrophage colony-stimulating factor, the proliferation rate increased and reached the peak after 2 days in culture and then gradually decreased because of density-dependent inhibition. At cell proliferation stage, approximately 80% of the GMIR1 cells exhibited glutamate (Glu)- and KA-induced inward currents at cell proliferation stage, whereas only 22.5% of the cells showed responsiveness to Glu and KA at cell quiescent stage. Furthermore, the mean amplitudes of inward currents induced by Glu and KA at cell proliferation stage (13.8+/-3.0 and 8.4+/-0.6 pA) were significantly larger than those obtained at cell quiescent stage (4.7+/-0.8 and 6.2+/-1.2 pA). In the GMIR1 cells, KA-induced inward currents were markedly inhibited by (RS)-3-(2-carboxybenzyl) willardiine (UBP296), a selective antagonist for KA receptors. The KA-responsive cells also responded to (RS)-2-amino-3-(3-hydroxy-5-tert-butylisoxazol-4-yl) propanoic acid (ATPA), a selective agonist for GluR5, in both GMIR1 cells and primary cultured rat microglia. Furthermore, mRNA levels of the KA receptor subunits, GluR5 and GluR6, at the cell proliferation stage were significantly higher than those at the cell quiescent stage. Furthermore, the immunoreactivity for GluR6/7 was found to increase in activated microglia in the post-ischemic hippocampus. These results strongly suggest that microglia have functional KA receptors mainly consisting of GluR5 and GluR6, and the expression levels of these subunits are closely regulated by the cell cycle mechanism.

  84. The intra-arterial injection of microglia protects hippocampal CAI neurons against global ischemia-induced functional deficits in rats. Reviewed

    HAYASHI Yoshinori, TOMIMATSU Yoshirou, SUZUKI Hiromi, YAMADA Jun, WU Ziqiang, YAO Hngping, KAGAMIISHI Yoshifumi, TATEISHI Narito, SAWADA Makoto, NAKANISHI Hiroshi

    Neuroscience   Vol. 142 ( 1 ) page: 87-96   2006.9

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    In the present study, we have attempted to elucidate the effects of the intra-arterial injection of microglia on the global ischemia-induced functional and morphological deficits of hippocampal CA1 neurons. When PKH26-labeled immortalized microglial cells, GMIR1, were injected into the subclavian artery, these exogenous microglia were found to accumulate in the hippocampus at 24 h after ischemia. In hippocampal slices prepared from medium-injected rats subjected to ischemia 48 h earlier, synaptic dysfunctions including a significant reduction of synaptic responses and a marked reduction of long-term potentiation (LTP) of the CA3-CA1 Schaffer collateral synapses were observed. At this stage, however, neither significant neuronal degeneration nor gliosis was observed in the hippocampus. At 96 h after ischemia, there was a total loss of the synaptic activity and a marked neuronal death in the CA1 subfield. In contrast, the basal synaptic transmission and LTP of the CA3-CA1 synapses were well preserved after ischemia in the slices prepared from the microglia-injected animals. We also found the microglial-conditioned medium (MCM) to significantly increase the frequency of the spontaneous postsynaptic currents of CA1 neurons without affecting the amplitude, thus indicating that MCM increased the provability of the neurotransmitter release. The protective effect of the intra-arterial injected microglia against the ischemia-induced neuronal degeneration in the hippocampus was substantiated by immunohistochemical and immunoblot analyses. Furthermore, the arterial-injected microglia prevented the ischemia-induced decline of the brain-derived neurotrophic factor (BDNF) levels in CA1 neurons. These observations strongly suggest that the arterial-injection of microglia protected CA1 neurons against the ischemia-induced neuronal degeneration. The restoration of the ischemia-induced synaptic deficits and the resultant reduction of the BDNF levels in CA1 neurons, possibly by the re

  85. *Cellular and Molecular Mechanisms of Parkinson's Disease: Neurotoxins, Causative Genens, and Inflammatory Cytokines. Invited Reviewed

    NAGATSU Toshiharu, SAWADA Makoto

    Cellular and Molecular Neurobiology   Vol. 26 ( 4-6 ) page: 781-802   2006.7

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    1. Parkinson's disease (PD) is considered to be an aging-related neurodegeneration of catecholamine (CA) systems [typically A9 dopamine (DA) neurons in the substantia nigra and A6 noradrenaline (NA) neurons in the locus coeruleus]. The main symptom is movement disorder caused by a DA deficiency at the nerve terminals of fibers that project from the substantia nigra to the striatum. Most PD is sporadic (sPD) without any hereditary history. sPD is speculated to be caused by some exogenous or endogenous substances that are neurotoxic toward CA neurons, which toxicity leads to mitochondrial dysfunction and subsequent oxidative stress resulting in the programmed cell death (apoptosis or autophagy) of DA neurons. 2. Recent studies on the causative genes of rare familial PD (fPD) cases, such as alpha-synuclein and parkin, suggest that dysfunction of the ubiquitin-proteasome system (UPS) and the resultant accumulation of misfolded proteins and endoplasmic reticulum stress may cause the death of DA neurons. 3. Activated microglia, which accompany an inflammatory process, are present in the nigro-striatum of the PD brain; and they produce protective or toxic substances, such as cytokines, neurotrophins, and reactive oxygen or nitrogen species. These activated microglia may be neuroprotective at first in the initial stage, and later may become neurotoxic owing to toxic change to promote the progression toward the death of CA neurons.4. All of these accumulating evidences on sPD and fPD points to a hypothesis that multiple primary causes of PD may be ultimately linked to a final common signal-transduction pathway leading to programmed cell death, i.e., apoptosis or autophagy, of the CA neurons.

  86. Molecular mechanism of the relation of monoamine oxidase B and its inhibitors to Parkinson's disease: possible implications of glial cells Invited Reviewed

    Nagatsu, T. and Sawada, M.

    J Neural Transm Suppl   Vol. 71   page: 53-65   2006

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    Monoamine oxidases A and B (MAO A and MAO B) are the major enzymes that catalyze the oxidative deamination of monoamine neurotaransmitters such as dopamine (DA), noradrenaline, and serotonin in the central and peripheral nervous systems. MAO B is mainly localized in glial cells. MAO B also oxidizes the xenobiotic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to a parkinsonism-producing neurotoxin, 1-methyl-4-phenyl-pyridinium (MPP+). MAO B may be closely related to the pathogenesis of Parkinson's disease (PD), in which neuromelanin-containing DA neurons in the substantia nigra projecting to the striatum in the brain selectively degenerate. MAO B degrades the neurotransmitter DA that is deficient in the nigro-striatal region in PD, and forms H2O2 and toxic aldehyde metabolites of DA. H2O2 produces highly toxic reactive oxygen species (ROS) by Fenton reaction that is catalyzed by iron and neuromelanin. MAO B inhibitors such as L-(-)-deprenyl (selegiline) and rasagiline are effective for the treatment of PD. Concerning the mechanism of the clinical efficacy of MAO B inhibitors in PD, the inhibition of DA degradation (a symptomatic effect) and also the prevention of the formation of neurotoxic DA metabolites, i.e., ROS and dopamine derived aldehydes have been speculated. As another mechanism of clinical efficacy, MAO B inhibitors such as selegiline are speculated to have neuroprotective effects to prevent progress of PD. The possible mechanism of neuroprotection of MAO B inhibitors may be related not only to MAO B inhibition but also to induction and activation of multiple factors for anti-oxidative stress and anti-apoptosis: i.e., catalase, superoxide dismutase 1 and 2, thioredoxin, Bcl-2, the cellular poly(ADP-ribosyl)ation, and binding to glyceraldehydes-3-phosphate dehydrogenase (GAPDH). Furthermore, it should be noted that selegiline increases production of neurotrophins such as nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and glial ce

  87. Tonic-clonic seizures induce division of neuronal progenitor cells with concomitant changes in expression of neurotrophic factors in the brain of pilocarpine-treated mice Reviewed

    HAGIHARA Hideo, HARA Mizumi, TSUNEKAWA Kyoko, NAKAGAWA Yukinori, SAWADA Makoto, NAKANO Kiwako

    Molecular Brain Research   Vol. 139 ( 2 ) page: 258-266   2005

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    Epileptic seizures cause severe and long-lasting events on the architecture of the brain, including neuronal cell death, accompanied neurogenesis, reactive gliosis, and mossy fiber sprouting. However, it remains uncertain whether these functional and anatomical alterations are associated with the development of hyperexcitability, or as inhibitory processes. Neurotrophic factors are probable mediators of these pathophysiological events. The present study was designed to clarify the role of various neurotrophic factors on the pilocarpine model of seizures. At 4 h following pilocarpine-induced seizures, expression of NGF, BDNF, HB-EGF, and FGF-2 increased only in the mice manifesting tonic-clonic convulsions and not in mice without seizures. NT-3 expression decreased in pilocarpine-treated mice experiencing seizures, tonic-clonic or not, compared to mice with no seizures. Neuronal cell damage, which was evident by Fluoro-Jade B staining, was observed within 24 h in the mice exhibiting tonic-clonic seizures, followed by an increase in the number of BrdU-positive cells and glial cells, which were evident after 2 days. None of these pathophysiological changes occurred in the mice which showed no seizures, although they were injected with pilocarpine, nor in the activated epilepsy-prone EL mice, which experienced repeated severe seizures. Together, these results suggest that neuronal damage occurring in the brain of the mice manifesting tonic-clonic seizures is accompanied by neurogenesis. This sequence of events may be regulated through changes in expression of neurotrophic factors such as NGF, BDNF, HB-FGF, and NT-3.

  88. A lentiviral expression system demonstrates that L* protein of Theiler's murine encephalomyelitis virus (TMEV) has an anti-apoptotic effect in a macrophage cell line Reviewed

    HIMEDA Toshiki, OHARA Yoshiro, ASAKURA Kunihiko, KONTANI Yasuhide, SAWADA Makoto

    Microb Pathogenesis   Vol. 38 ( 5-6 ) page: 201-207   2005

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    DA subgroup strains of TMEV persist in the CNS of infected mice and induce demyelination. The mechanism(s) of virus persistence and demyelination remains unknown. DA subgroup strains synthesize a 17-kDa protein, called L*, from an initiation site out-of-frame with the polyprotein. The previous study using a mutant virus, DAL*-1 (in which the L* AUG is substituted by an ACG) showed that L* has an anti-apoptotic effect in a macrophage cell line, P388D1. Therefore, we established P388D1 cells that continuatively express L*, in order to confirm its role in TMEV-induced apoptosis. The anti-apoptotic activity of L* may be important in TMEV pathogenesis.

  89. Localization of cyclooxygenase-2 induced following traumatic spinal cord injury Reviewed

    ADACHI Kayo, Yimin Yu, SATAKE Kotaro, MATSUYAMA Yukihiro, ISHIGURO Naoki, SAWADA Makoto, HIRATA Yoko, KIUCHI Kazutoshi

    Neuroscience Research   Vol. 51   page: 73-80   2005

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    OBJECTIVE: Up-regulation of cyclooxygenase-2 (COX-2), a key enzyme in the synthesis of prostaglandins (PGs), is postulated to be involved in pathological processes of acute spinal cord injury (SCI). In the present study, we sought to clarify temporal and spatial expression patterns of the COX-2 gene induced in the spinal cord after traumatic insults using a weight-drop technique. RESULTS: Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that COX-2 transcription in the spinal cord began to increase within 30 min, peaked at 3 h after injury. Western blotting analysis indicated that the deglycosylated COX-2 protein significantly increased 6 h after injury. Double-immunofluorescent staining analysis showed that COX-2 immunoreactivity was present only in endothelial cells of blood vessels, but not in neurons, astrocytes, monocytes, macrophages, or microglia 6 h after injury. CONCLUSIONS: The results suggested that COX-2 gene induction seems not to require any new protein synthesis and that its expression in endothelial cells may be a component of an inflammatory process after traumatic SCI.

  90. Cytokine production of activated microglia and decrease in neurotrophic factors of neurons in the hippocampus of Lewy body disease brains Reviewed

    Imamura, K., Hishikawa, N., Ono, K., Suzuki, H., Sawada, M., Nagatsu, T., Yoshida, M., Hashizume, Y.

    Acta Neuropathol   Vol. 109   page: 141-150   2005

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    Dementia is a frequent complication of Parkinsons disease (PD) and usually occurs late in the protracted course of the illness. We have already reported numerous MHC class II-positive microglia in the hippocampus in PD patients, and that this phenomenon may be responsible for functional changes in the neurons and the cognitive decline in PD patients. In this study, we have investigated the distribution of activated microglia and the immunohistochemical and the mRNA expression of several cytokines and neurotrophic factors of the hippocampus in PD and dementia with Lewy bodies (DLB). The brains from five cases of PD and five cases of DLB that were clinically and neuropathologically diagnosed, and those from four normal controls (NC) were evaluated by immunohistochemistry using anti-HLA-DP, -DQ, -DR (CR3/43), anti--synuclein, anti-brain-derived neurotrophic factor (BDNF), and anti-glial fibrillary acidic protein antibodies. In addition, the mRNA expressions of cytokines (IL-1, IL-1, TNF-, IL-6, TGF-) and neurotrophic factors (BDNF, GDNF, NGF, NT-3) of these brains were evaluated by the reverse transcription-PCR method. MHC class II-positive microglia were distributed diffusely in the hippocampus of PD and DLB brains. Although the cytoplasm of pyramidal and granular cells of the hippocampus in NC brains was strongly stained by anti-BDNF antibodies, it was only weakly stained in PD and DLB brains. The mRNA expression of IL-6 was significantly increased in the hippocampus of PD and DLB brains, and that of BDNF was significantly decreased in the hippocampus of DLB brains. The increased number of activated microglia and the production of neurotrophic cytokines such as IL-6, together with the decreased expression of the neurotrophic factors of neurons in the hippocampus of PD and DLB brains, may be related to functional cellular changes associated with dementia.

  91. Amyloid-beta peptides induce cell proliferation and macrophage colony-stimulating factor expression via the PI3-kinase/Akt pathway in cultured Ra2 microglial cells Reviewed

    ITO Sachiko, SAWADA Makoto, HANEDA Masataka, FUJII S, OHHASHI Kentaro, KIUCHI Kazatoshi, TAKAHASHI Masahide, ISOBE Ken-ichi

    FEBS Letters   Vol. 579   page: 1995-2000   2005

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    Alzheimer's disease is characterized by numerous amyloid-beta peptide (Abeta) plaques surrounded by microglia. Here we report that Abeta induces the proliferation of the mouse microglial cell line Ra2 by increasing the expression of macrophage colony-stimulating factor (M-CSF). We examined signal cascades for Abeta-induced M-CSF mRNA expression. The induction of M-CSF was blocked by a phosphatidylinositol 3 kinase (PI3-kinase) inhibitor (LY294002), a Src family tyrosine kinase inhibitor (PP1) and an Akt inhibitor. Electrophoretic mobility shift assays showed that Abeta enhanced NF-kappaB binding activity to the NF-kappaB site of the mouse M-CSF promoter, which was blocked by LY294002. These results indicate that Abeta induces M-CSF mRNA expression via the PI3-kinase/Akt/NF-kappaB pathway.

  92. Peripheral injection of lipopolysaccharide enhances expression of inflammatory cytokines in murine locus coeruleus: possible role of increased norepinephrine turnover Reviewed

    KANEKO YokoS, MORI Kenji, NAKASHIMA Akira, SAWADA Makoto, NAGATSU Ikuko, OTA Akira

    Journal of Neurochemistry   Vol. 94 ( 2 ) page: 393-404   2005

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    Cytokines and catecholamines are known to constitute a significant portion of the regulatory neuroimmune networks involved in maintaining homeostasis in the central nervous system (CNS). Although we have already reported an increase in norepinephrine (NE) turnover within the locus coeruleus (LC) at 2 and 4 h after the intraperitoneal (i.p.) injection of lipopolysaccharide (LPS), the implication of this increase remains unclear. In view of evidence that norepinephrine (NE) acts in an anti-inflammatory manner by way of negatively regulating pro-inflammatory cytokine expression, we examined the inflammatory cytokine expression levels in the LC of C3H/HeN mice (male, 8 weeks old) after an i.p. LPS injection. The mRNA expression levels of the genes encoding IL-1beta and TNF-alpha within the LC increased during the first 2 h, and showed two peaks, the first at 4 h and the second lesser one at 15 h after the LPS injection. Microglia, which are one of the major cell types that produce pro-inflammatory cytokines in the CNS, were isolated from mouse neonate brains in order to clarify more precisely the relationship between the changes in NE content and the up-regulation of inflammatory cytokines in the LC. Simultaneous incubation of microglia with LPS and NE enhanced the expression of IL-1beta at both mRNA and protein levels, but reduced the mRNA and protein levels of TNF-alpha. These data support the hypothesis that NE negatively regulates the expression of pro-inflammatory cytokine expression, at least in the case of TNF-alpha, which action could contribute to the observed anti-inflammatory properties of NE. This report, based on the results of both in vivo and in vitro experiments, is the first to suggest a relationship between NE content and cytokine expression levels in the CNS.

  93. Double-stranded RNA stimulates chemokine expression in microglia through vacuolar pH-dependent activation of intracellular signaling pathways Reviewed

    NAKAMICHI Kazuo, SAIKI Megumi, SAWADA Makoto, TAKAYAMA-ITO Mutsuyo, YAMAMURO Yutaka, MORIMOTO Kinjiro, KURANE Ichiro

    Journal of Neurochemistry   Vol. 95   page: 273-283   2005

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    During neurotropic virus infection, microglia act as a source of chemokines, thereby regulating the recruitment of peripheral leukocytes and the multicellular immune response within the CNS. Herein, we present a comprehensive study on the chemokine production by microglia in response to double-stranded RNA (dsRNA), a conserved molecular pattern of virus infection. Transcriptional analyses of chemokine genes revealed that dsRNA strongly induces the expression of CXC chemokine ligand 10 (CXCL10) and CC chemokine ligand 5 (CCL5) in microglia. We also observed that the dsRNA stimulation triggered the activation of signaling pathways mediated by nuclear factor kappaB (NF-kappaB) and mitogen-activated protein kinases (MAPK), including extracellular signal-regulated kinases 1 and 2 (ERK1/2), p38, and c-Jun N-terminal kinase (JNK). The microglial CXCL10 response to dsRNA was induced via NF-kappaB, p38, and JNK pathways, whereas the dsRNA-induced CCL5 production was dependent on JNK, but not on the other signal-transducing molecules tested. In addition, the acidic environment of intracellular vesicles was required for the activation of cellular signaling in response to dsRNA. Taken together, these results suggest that the recognition of dsRNA structure selectively induces the CXCL10 and CCL5 responses in microglia through vacuolar pH-dependent activation of NF-kappaB and MAPK signaling pathways.

  94. A lentiviral expression system demonstrates that L* protein of Theiler's murine encephalomyelitis virus (TMEV) is essential for virus growth in a murine macrophage-like cell line Reviewed

    HIMEDA Toshiki, OHARA Yoshiro, ASAKURA Kunihiko, KONTANI Yasuhide, MURAKAMI Manabu, SUZUKI Hiromi, SAWADA Makoto

    Virus Research   Vol. 108   page: 23-28   2005

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    The DA subgroup strains of Theiler's murine encephalomyelitis virus (TMEV) synthesize L* protein, which is translated out of frame with the polyprotein from an alternative AUG, 13 nucleotides downstream from the authentic polyprotein AUG. By a 'loss of function' experiment using a mutant virus, DAL*-1, in which the L* AUG is mutated to an ACG, L* protein is shown to play an important role in virus persistence, TMEV-induced demyelination, and virus growth in macrophages. In the present study, we established an L* protein-expressed macrophage-like cell line and confirmed the importance of L* protein in virus growth in this cell line.

  95. Inhibition of inflammatory cytokine secretion from mouse microglia cells by DHMEQ, an NF-kappaB inhibitor Reviewed

    TAKATSUNA Hiroshi, MORITA Shigeru, NAGATSU Toshiharu, SAWADA Makoto, UMEZAWA Kazuo

    Biomedecine & Pharmacotherapy   Vol. 59   page: 318-322   2005

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    Activation of microglia has been implicated in various neurodegenerative disorders, and thus the inhibition of microglial activity may suppress these disorders. Earlier we designed and synthesized an NF-kappaB inhibitor, dehydroxymethylepoxyquinomicin (DHMEQ) that showed anti-inflammatory and anti-tumor activities in vivo. In the present research, we studied whether DHMEQ would inhibit the activation of mouse microglial cells. DHMEQ inhibited lipopolysaccharide (LPS)-induced activation of NF-kappaB in an electrophoresis mobility shift assay. It also inhibited LPS-induced secretions of TNF-alpha and IL-6 from mouse microglial cell line 6-1 cells.

  96. Vitamin K2 ameliorates experimental autoimmune encephalomyelitis in Lewis rats Reviewed

    MORIYA Masayuki, NAKATSUJI Yuji, OKUNO Tatsusada, HAMASAKI Toshimitsu, SAWADA Makoto, SAKODA Saburo

    Journal of Neuroimmunology   Vol. 59   page: 318-322   2005

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    Vitamin K2 (VK2), which has been in wide use for the management of hypoprothrombinemia and osteoporosis in Japan, was tested for its efficacy on experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). The severity of EAE was significantly ameliorated by the prophylactic administration of VK2, though it was not effective when given after the onset. Inflammatory cellular infiltration and the expression of both MHC class II and inducible nitric oxide synthase (iNOS) were reduced in the spinal cords of VK2-treated rats with EAE. The inhibitory effect of VK2 on the iNOS expression in glial cells was also observed in vitro. Considering the long use of VK2 without noticeable untoward effects, it may be applicable to the patients with MS.

  97. Rabies virus-induced activation of MAPK and NF-kappaB signaling pathways regulates expression of CXC and CC chemokine ligands in microglia Reviewed

    NAKAMICHI Kazuo, SAIKI Megumi, SAWADA Makoto, YAMAMURO Yutaka, MORIMOTO Kinjiro, KURANE Ichiro

    Journal of Virology   Vol. 79   page: 11801-11812   2005

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    Following virus infection of the central nervous system, microglia, the ontogenetic and functional equivalents of macrophages in somatic tissues, act as sources of chemokines, thereby recruiting peripheral leukocytes into the brain parenchyma. In the present study, we have systemically examined the growth characteristics of rabies virus (RV) in microglia and the activation of cellular signaling pathways leading to chemokine expression upon RV infection. In RV-inoculated microglia, the synthesis of the viral genome and the production of virus progenies were significantly impaired, while the expression of viral proteins was observed. Transcriptional analyses of the expression profiles of chemokine genes revealed that RV infection, but not exposure to inactivated virions, strongly induces the expression of CXC chemokine ligand 10 (CXCL10) and CC chemokine ligand 5 (CCL5) in microglia. RV infection triggered the activation of signaling pathways mediated by mitogen-activated protein kinases, including p38, extracellular signal-regulated kinases 1 and 2 (ERK1/2), and c-Jun N-terminal kinase, and nuclear factor B (NF-B). RV-induced expression of CXCL10 and CCL5 was achieved by the activation of p38 and NF-B pathways. In contrast, the activation of ERK1/2 was found to down-regulate CCL5 expression in RV-infected microglia, despite the fact that it was involved in partial induction of CXCL10 expression. Furthermore, NF-B signaling upon RV infection was augmented via a p38-mediated mechanism. Taken together, these results indicate that the strong induction of CXCL10 and CCL5 expression in microglia is precisely regulated by the activation of multiple signaling pathways through the recognition of RV infection.

  98. Inflammatory process in Parkinson's disease: role for cytokines Invited Reviewed

    Nagatsu, T., Sawada, M.

    Curr Pharm Res   Vol. 11   page: 999-1016   2005

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    Parkinson's disease (PD) is a movement disorder caused by degeneration of the nigrostriatal dopamine (DA) neurons in the substantia nigra pars compacta and the resultant deficiency in the neurotransmitter DA at the nerve terminals in the striatum. We and other investigators found increased levels of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-6, and decreased levels of neurotrophins such as brain-derived neurotrophic factor (BDNF) in the nigrostriatal region of postmortem brains and/or in the ventricular or lumbar cerebrospinal fluid (CSF) from patients with sporadic PD, and in animal models, such as 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)- and 6-hydroxydopamine (6-OHDA)-induced PD. These changes in cytokine and neurotrophin levels may be initiated by activated microglia, which may then promote apoptotic cell death and subsequent phagocytosis of DA neurons. Cytokines as pleiotropic factors, promote signals that either lead to cell death or exert neuroprotective effects. The discovery of toxic changes in trophic microglia by M. Sawada and co-workers is important to this point. Ultimately, microglial cells may regulate cellular changes that cause either harm or benefit by producing cytokines or neurotrophins depending upon the primary cause and the circumstances during the inflammatory process of PD.

  99. Superoxide production at phagosomal cup/phagosome through betal protein kinase C during FcgammaR-mediated phagocytosis in microglia.

    T.Ueyama, M.R.Lennartz, Y.Noda, T.Kobayashi, Y.Shirai, K.Rikitake, T.Yamasaki, S.Hayashi, N.Sakai, H.Seguchi, M.Sawada, H.Sumitomo and N.Saito

    J. Immunol.   Vol. 173 ( 7 ) page: 4582-4589   2004

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  100. Pepstatin A induces extracellular acidification distinct from aspartic protease inhibition in microglial cell lines.

    M.Okada, S.Irie, M.Sawada, R.Urae, A.Urae, N.Iwata, N.Ozaki, K.Akazawa andH.Nakanishi

    Glia   Vol. 43 ( 2 ) page: 167-174   2003

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  101. Distribution of major histocompatibility complex class II-positive microglia and cytokine profile of Parkinson's disease brains.

    K.Imamura, N.Hishikawa, M.Sawada, T.Nagatsu, M.Yoshida,and Y.Hashizume

    Acta Neuropathol(Berl)   Vol. 106 ( 6 ) page: 518-526   2003

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  102. Microglial cell cycle-associated proteins control microglial proliferation in vivo and in vitro and are regulated by GM-CSF and density-dependent inhibition.

    K.Koguchi, Y.Nakatsuji, T.Okuno, M.Sawada and S.Sakoda

    J. Neurosci Res   Vol. 74 ( 6 ) page: 898-905   2003

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  103. Preservation of hematopoietic properties in transplanted bone marrow cells in the brain.

    K.Ono, K.Yoshihara, H.Suzuki, K.F.Tanaka, T.Takii, K.Onozaki and M.Sawada

    J. Neurosci Res   Vol. 72 ( 4 ) page: 503-507   2003

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  104. The HIV-1 Nef protein and phagocyte NADPH oxidase activation.

    F.Vilhardt, O.Plastre, M.Sawada, K.Suzuki, M.Wiznerowicz. E.Kiyokawa, D.Trono and K.H.Krause

    J.Biol Chem   Vol. 277 ( 44 ) page: 42136-42143   2002

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  105. Distinct Cell death mechamisms by Theiler's murine encephalomyelitis virus (TMEV) infection in microglia and macrophage

    Y.Ohara, T.Himeda, K.Asakura and M.Sawada

    Netrosci Lett.   Vol. 327 ( 1 ) page: 41-44   2002

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  106. Existence of functional b1-and b2-adrenergic receptors on microglia

    K.F.Tanaka, H.Kashima, H.Suzuki, K.Ono and M.Sawada

    J Neurosci. Res.   Vol. 70 ( 2 ) page: 232-237   2002

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  107. Glial cell line-derived neurotrophic factor enhances survival of GM-CSF dependent rat GMIR1-microglial cells

    K.Salimi, K.Moser, B.Zassler, M.Reindl, N.Embacher, C.Schermer, C.Weis, J.Marksteiner, M.Sawada and C. Humpel

    Neurosci Res.   Vol. 43 ( 3 ) page: 221-229   2002

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  108. alpha-Synuclein forms a complex with transcription factor Elk-1

    A.Iwata, S.Miura, I Kanazawa, M.Sawada and N.Nukina

    J. Neurochem.   Vol. 77 ( 1 ) page: 239-252   2001

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  109. Preservation of neurotrophin expression in microglia that migrate into the gerbil's brain across the blood brain barrier

    H.Suzuki, F.Imai, T.Kanno and M.Sawada

    Neurosci. Lett.   Vol. 312 ( 2 ) page: 95-98   2001

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  110. ミクログリア 21世紀の神経免疫学展望

    医学の歩み   Vol. 別冊   page: 32-35   2001

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  111. ミクログリアの脳保護作用と脳特異的薬物輸送

    現代医学   Vol. 48 ( 3 ) page: 615-620   2001

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  112. ミクログリア細胞を用いた脳疾患の治療戦略

    神経研究の進歩   ( 45 ) page: 63-72   2001

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  113. Activation mechanism of brain microglia in patients with diffuse neurofibrillary tangles with calcification: a comparison with Alzheimer disease

    K.Imamura, M.Sawada, N.Ozaki, H.Naito, N.Iwata, R.Ishsihara, T.Takeuchi and H.Shibayama

    Alzheimer Dis. Assoc. Disord.   Vol. 15 ( 1 ) page: 45-50   2001

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  114. Age-dependent and tissue-specific CAG rereat instability occurs in mouse knock-in for a mutant Huntington's disease gene

    H.Ishiguro, K.Yamada, H.Sawada, K.Nishii, N.Ichino, M.Sawada, Y.Kurosawa,N.Matsushita, K.Kobayashi, J.Goto,H.Hashida, N.Masuda, I.Kanazawa and T.Nagatsu

    J. Neurosci. Res.   Vol. 65 ( 4 ) page: 289-297   2001

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  115. Histamine production by cultured microglial cells of the mouse

    Y.Katoh, M.Niimi, Y.Yamamoto, T.Kawamura, T.Morimoto-Ishizuka, M.Sawada, H.Takemori and A.YamatodaniH.Takemori and A.Yamatodani

    Neurosci. Lett.   Vol. 305 ( 3 ) page: 181-184   2001

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  116. Differentiated regulation of allo-antigen presentation by different types of murine microglial cell lines.

    T.Kanzawa, M.Sawada, K.Kato, K.Yamamoto, H.Mori and R.Tanaka

    J Neurosci Res   Vol. 62 ( 3 ) page: 383-388   2000

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  117. Impaired cytokine production by peripheral blood mononuclear cells and monocytes/macrophages in Parkinson's disease.

    Y.Hasegawa, T.Inagaki, M.Sawada and A.Suzumura

    Acta Neurol. Scand.   Vol. 101 ( 3 ) page: 159-164   2000

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  118. Increased soluble tumor necrosis factor receptor levels in the serum of elderly people

    Y.Hasegawa, M.Sawada, N.Ozaki, T.Inagaki and A.Suzumura

    Gelontology   Vol. 46 ( 4 ) page: 185-188   2000

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  119. Cell cycle regulation and apoptosis

    Clin. Neurosci   Vol. 18   page: 407-409   2000

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  120. Distinctive regulation of antigen presentation by different type of murine microglial clones

    J. Neurosci Res.   Vol. 62   page: 383-388   2000

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  121. Temporal fluctuations of voltage-gated proton currents in rat spinal microglia via pH-dependent and-independent mechanisms

    H.Morihata, J.Kawasaki, H.Sakai, M.Sawada, T.Tsutada and M.Kuno

    Neurosci. Res.   Vol. 38 ( 3 ) page: 265-271   2000

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  122. 細胞周期とアポトーシス

    Clin. Neurosci   Vol. 18   page: 407-409   2000

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  123. 細胞を使って脳の疾患を治療する試み

    治療   Vol. 82   page: 2200-2202   2000

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  124. Exogenous microglia enter the brain and migrate into ischaemic hippocampal lesions

    Neurosci Lett   Vol. 272   page: 127-130   1999

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  125. Brain cytokine network and microglia

    Jpn. J. Neuropsychopharmacol.   Vol. 19   page: 151-154   1999

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  126. Apoptosis and Necrosis

    Seibutusiryoubunnseki   Vol. 22   page: 93-100   1999

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  127. Serial analysis of gene expression in a microglial cell line

    Glia   Vol. 28   page: 265-71   1999

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  128. Effects of phosphodiesterase inhibitors on cytokine production by microglia

    Mult Scler   Vol. 5   page: 126-133   1999

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  129. Effects of vesnarinone on cytokine production and activation of murine microglia

    Life Sci   Vol. 64   page: 1197-1203   1999

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  130. Ibudilast suppresses TNFalpha production by glial cells functioning mainly as type (]G0003[) phosphodiesterase inhibitor in the CNS

    Brain Res   Vol. 837   page: 203-212   1999

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  131. Interliukin-10 inhibits both production of cytokines and expression of cytokine receptors in microglia

    J Neurochem   Vol. 72   page: 1466-1471   1999

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  132. Migration of exogenous immature hematopoietic cells into adult mouse brain parenchyma under GFP-expressing bone marrow

    Biochem Biophys Res Commun   Vol. 262   page: 610-614   1999

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  133. Enhanced Fos expression in the hippocampus of El mice after short-term vestibular stimulation

    Neurosci Lett   Vol. 271   page: 105-108   1999

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  134. 脳のサイトカインネットワークとミクログリアの脳での役割

    日本神経精神薬理学雑誌   Vol. 19   page: 151-154   1999

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  135. アポトーシスとネクローシス

    生物試料分析   Vol. 22   page: 93-100   1999

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  136. ミクログリアの新規な性質と脳での役割-ミクログリアは脳で何をしているか

    細胞工学   Vol. 18   page: 550-558   1999

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  137. "Tryptophan and its metabolite, kynurenine, stimulate expression of nerve growth factor in cultured mouse astroglial cells."

    Neurosci. Lett.   Vol. 244   page: 17-20   1998

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  138. Pathophysiological significance of cytokine network in the brain

    Japan Scienctific Societies Press     page: 217-228   1998

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  139. Appotosis and Microglia

    Nippon Yakurigaku Zasshi   Vol. 112   page: 15-19   1998

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  140. Differentiation-specific mRNA expression of a mouse bibptential glial cell line

    Neurosci. Lett   Vol. 258   page: 21-24   1998

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  141. Cytokines and Neural differentiation

    Clinical Neuroscience   Vol. 16   page: 406-408   1998

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  142. Insulin-like growth factor-I analogue prevents apoptosis mediated through an interleukin-Ib converting enzyme(caspase-1)-like protease of cerebellar external tranular layer neurons : developmental stage-specific mechanisms of neuronal cell death.

    Neuroscience   Vol. 84   page: 89-100   1998

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  143. Microglial NO induces delayed neuronal death following acute injury in the striatum

    Eur J Neurosci   Vol. 10   page: 1613-1620   1998

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  144. Production of interleukin-12 and expression of its receptors by murine microglia.

    Brain Research   Vol. 787   page: 139-142   1998

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  145. Brain-specific gene expression by immortalized microglial cell-mediated gene transfer in the mammalian brain.

    FEBS Lett.   Vol. 433   page: 37-40   1998

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  146. ミクログリアとアポートシス

    日本薬学雑誌   Vol. 112   page: 15-19   1998

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  147. 神経系の分化とサイトカイン

    Clinical Neuroscience   Vol. 16   page: 406-408   1998

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  148. Changes in gene expression in mouse astrocytes during long-term. serial cultivation

    Tissue Culture Research Communication   Vol. 16   page: 129-135   1997

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  149. Possible strategy of therapies against brain intractable diseases by brain-speciffic gene delivery technique with immortalized microglia

    Nippon-Iji-Shinpo   Vol. 3844   page: 28-30   1997

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  150. Expression of Ly-6C on microglia in the developing and adult mouse brain.

    Neurosci. Lett.   Vol. 239   page: 17-20   1997

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  151. Migration activity microglia and macrophages into rat brain.

    Neurosci. Lett.   Vol. 237   page: 49-52   1997

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  152. Cytokine network in the brain

    Tissue Culture Research Communication   Vol. 42 ( 3 ) page: 504-511   1997

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  153. ミクログリア細胞株を用いた脳疾患の治療の可能性

    日本醫事新報   Vol. 3844   page: 28-30   1997

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  154. 脳のサイトカインネットワーク

    蛋白質核酸酵素   Vol. 42 ( 3 ) page: 504-511   1997

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  155. ER-MP抗原陽性ミクログリアの存在について

    Journal of Neural Transmission   Vol. 15   page: 221-227   1996

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  156. Expression pattern of messenger RNAs for prostanoid receptors in glial cell cultures

    Brain Research   Vol. 707   page: 282-287   1996

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  157. Presence of ER-MP antigen positive microglia

    Tissue Culture Research Communications   Vol. 15   page: 221-227   1996

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  158. Selective induction of interleukin-6 in mouse microglia by granulocyte-macrophage colony-stimulationg factor

    Brain Research   Vol. 713   page: 192-198   1996

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  159. ミクログリアの発生と多様性

    細胞   Vol. 27 ( 5 ) page: 193-198   1995

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  160. Induction of functional interleukin-2 receptor in mouse microglia

    Journal of Neurochemistry   Vol. 64   page: 1973-1979   1995

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  161. Programmed cell death of PC12 induced by adenovirus EIA

    Nueroscience Letters   Vol. 191   page: 173-176   1995

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  162. cDNA cloning of a thromboxane A2 receptor from rat astrocytes

    Biochimica et Biophysica Acta   Vol. 1265   page: 220-223   1995

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  163. Cytokine network in the central nervous system and its roles in growth and differentiation of glial and neuronal cells

    International Journal of Developmental Neuroscience   Vol. 13 ( 3・4 ) page: 253-264   1995

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  164. Apoptosis-regulating factors which bind adenovirus E1A and E1B proteins

    Tissue Culture Research Communication   Vol. 13 ( 16 ) page: 1884-1889   1995

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  165. Neoptenin in immunological reactions in cancer and in the brain-- Possible involuement of macrophage and microglia

    Pteridnes   Vol. 6   page: 190-193   1995

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  166. Insulin prevents apoptosis of external granular layer neurons in rat cerebellar slice cultures

    Neuroscience Letters   Vol. 199   page: 37-40   1995

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  167. Developmental origin and multi-functional subtypes of microglia

    The Cell   Vol. 27 ( 5 ) page: 193-198   1995

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  168. Expression of glutamate transporters in cultured glial cells

    Neuroscience Letters   Vol. 188   page: 140-142   1995

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  169. The bipotential glial progenitor cell line can develop into both oligodendrocytes and astrocytes in the mouse forebrain

    Neuroscience Letters   Vol. 188 ( 1 ) page: 1-4   1995

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  170. アデノウイルスE1AとE1Bに結合するアポトーシス調節因子

    実験医学   Vol. 13 ( 16 ) page: 1884-1889   1995

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  171. Interleukin-4 induces proliferation and activation of microglia but suppresses their induction of class II major histocompatibility complex antigen expression

    Journal of Neuroimmunology   Vol. 53   page: 209-218   1994

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  172. Expression of cytokines during glial differentiation

    Brain Research   Vol. 656   page: 141-146   1994

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  173. Production of interleukin-10 by mouse glial cells in culture

    Biochemical and Biophysical Research Communications   Vol. 205 ( 3 ) page: 1907-1915   1994

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  174. Induction of MHC class II antigen expression on murine microglia by interleukin-3

    Journal of Neuroimmunology   Vol. 55   page: 119-125   1994

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  175. Production of interleukin-5 by mouse astrocytes and microglia in culture.

    Neuroscience Letters   ( 155 ) page: 175-178   1993

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  176. Brain vessels near muscle autografts are sites for entry of isogeneic macrophage into brain

    Experimental Neurology   Vol. 124   page: 219-230   1993

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  177. Gene expression commonly observed in both immune and neruous system

    "Annual Review of Immunology, 1993"     page: 134-142   1993

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  178. Effects of microglia-derived cytokines on astrocyte proliferation. (共著)

    Restoractive Newology and Neuvoscience   ( 5 ) page: 347-352   1993

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  179. Transforming Growth Factor-β Suppresses Activation and Proliferation of Microglia in Vitro. (共著)

    Journal of Immunology   ( 151 ) page: 2150-2158   1993

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  180. Expression of cytokine receptors in cultured neural and glial cells.

    Neuroscience Letters   ( 160 ) page: 131-134   1993

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  181. Regulation of astrocyte proliferation by prostaglandin E2 and the α subtype of protein kinase C.

    Brain Research   ( 613 ) page: 67-73   1993

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  182. 免疫系と神経系に共通する遺伝子発現

    Annual Review免疫1993     page: 134-142   1993

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  183. TNF alpha induces IL-6 production by astrocytes but not by microglia.

    Brain Research   Vol. 583 ( 1 ) page: 296-299   1992

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  184. "A PCR method for the quantitative assessment of mRNA for laminin A, B1, and B2 chains(共著)"

    Kidney International   ( 42 ) page: 764-769   1992

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  185. Establishment of mouse oligodendrocyte/type-2 astrocyte lineage cell line by transfection with origin-defective Simian virus 40 DNA(共著)

    Cell Structure and Function   ( 17 ) page: 325-333   1992

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  186. Induction of cytokines in glial cells by trans activator of human T cell lymphotropic virus type I.

    FEBS Letters   Vol. 313 ( 1 ) page: 47-50   1992

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  187. Down regulation of CD4 expression in cultured microglia by immunosuppressants and lipopolysaccharide.

    Biochemical and Biophysical Research Communications   Vol. 189 ( 2 ) page: 869-876   1992

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  188. 培養ミクログリアの免疫組織学的検討

    臨床神経学   Vol. 31 ( 2 ) page: 127-134   1991

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  189. Functional and immunohistochemical studies of cultured rat microglia. (共著)

    Clinical Neurology   Vol. 31 ( 2 ) page: 127-134   1991

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  190. NIHにおける相同組換えによるGene Targettingの現状

    蛋白質核酸酵素   Vol. 35 ( 13 )   1990

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  191. "Tyrosine hydroxylase, tryptophan hydroxylase, biopterin and neopterin in the brain of anorexia nervosa. (共著)"

    Journal of Neural Transmission   Vol. 80   page: 145-150   1990

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  192. Effects of colony stimulating factors on isolated microglia in vitro. (共著)

    Journal of Neuroimmunology   Vol. 30 ( 2-3 ) page: 111-120   1990

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  193. HTLV-I trans activator induces class I MHC antigen expression in glial cells.

    Journal of Virology   Vol. 64 ( 8 ) page: 4002-4006   1990

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  194. Activation and proliferation of the isolated microglia by colony stimulating factor-1 and possible involvement of protein kinase C.

    Brain Research   Vol. 509 ( 1 ) page: 119-124   1990

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  195. Production of granulocyte/macrophage colony-stimulating factor by cultured astrocytes. (共著)

    Biochemical and Biophysical Research Communications   Vol. 169 ( 2 ) page: 719-724   1990

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  196. Gene targeting at NIH

    "Proteins, Nucleic Acids and Enzymes"   Vol. 35 ( 13 )   1990

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  197. Production of tumor necrosis factor-alpha by microglia and astrocytes in culture.

    Brain Research   Vol. 491 ( 2 ) page: 394-397   1989

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  198. 神経伝達物質についての最近のアプローチ

    メディカル・イムノロジー   Vol. 16 ( 1 )   1988

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  199. Tryptophan hydroxylase activity in brain slices.

    International Journal of Biochemistry   Vol. 20 ( 10 ) page: 1033-1038   1988

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  200. Resent approach to neurotransmitters

    Medical Immunology   Vol. 16 ( 1 )   1988

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  201. ビオプテリンとネオプテリンの蛍光偏光測定

    蛋白質・核酸・酵素   Vol. 31   1987

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  202. Combined use of urinary pteridines measured by radioimmunoassays and polyamines as tumor markers. (共著)

    Biogenic Amines   Vol. 4/ ( 1 ) page: 433-443   1987

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  203. A factor inducing differentiation of the human nonocytic cell line U-937 produced by 12-O-tetradecanoylpholbol 13-acetate-treated U-937. (共著)

    Japanese Journal of Cancer Research   Vol. 78   page: 219-222   1987

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  204. "Tyrosine hydroxylase, tryptophan hydroxylase, biopterin and neopterin in postmortem brains from control and patients with Alzheimer-type dementia."

    Journal of Neurochemistry   Vol. 48   page: 760-764   1987

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  205. "Effects of thyrotropin releasing hormone and its analogue, DN1417, on biopterin concentration and tryptophan hydroxylation in rat raphe slices."

    Life Sciences   Vol. 41   page: 2733-2737   1987

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  206. Polarization fluoroimmunoassay of biopterin and neopterin(共著)

    "Proteins, Nucleic Acids, and Enzymes"   Vol. 31   1987

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  207. Stimulation of serotonin autoreceptor prevents the calcium-calmodulin-dependent increase of serotonin biosynthesis in rat raphe slices.

    Journal of Neurochemistry   Vol. 46   page: 963-967   1986

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  208. Preparation and cofactor activity of(6S)-tetrahydrobiopterin. (共著)

    Chemistry Express   Vol. 1 ( 7 ) page: 403-406   1986

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  209. (6R)-Tetra-hydrobiopterin increases the activity of tryptophan hydroxylase in rat raphe slices.

    Journal of Neurochemistry   Vol. 47   page: 1544-1547   1986

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  210. A sensitive assay of GTP cyclohydrolase I activity in rat and human tissues using radioimmunoassay of neopterin.

    Analytical Biochemistry   Vol. 154   page: 361-366   1986

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  211. Effects of adrenergic drugs on the activities of tryptophan hydroxylase in midbrain raphe slices of the rat.

    Neurochemistry International   Vol. 8 ( 3 ) page: 413-416   1986

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  212. "Changes in activities of tryptophan hydroxylase and cyclic AMP-dependent and calcium-calmodulin-dependent protein kinases in raphe serotonergic neurons of 5,7-dihydroxytryptamine-treated rats."

    Neurochemistry International   Vol. 7 ( 5 ) page: 761-763   1985

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  213. "Synthesis, localization and release of 5-hydroxytryptamine in neuroblastoma x glioma NG108-15 hybrid cells. (共著)"

    Brain Research   Vol. 361 ( 1 ) page: 77-90   1985

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  214. Tryptophan hydroxylase activity in the brains of controls and parkinsonian patients.

    Journal of Neural Transmission   Vol. 62   page: 107-115   1985

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  215. "Kinetic analysis of the inhibition of tryptophan hydroxylase, a pteridine=requiring monooxygenase, by oudenone and its derivatives."

    Biogenic Amines   Vol. 1   page: 121-178   1984

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  216. Radioimmunoassay for neopterin in body fluid and tissues. (共著)

    Analytical Biochemistry   Vol. 141   page: 472-480   1984

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  217. Elevated levels of polyamines and radioimmuoassayable 6-hydroxymethy1-pterin-like compound in urine from cancer patients. (共著)

    Biogenic Amines   Vol. 1   page: 51-62   1984

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  218. Polarization fluoroimmunoassay of biopterin and neopterin in human urine.

    Clinica Chimica Acta   Vol. 138   page: 275-282   1984

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  219. Serotonin in neuroblastoma x glioma NG108-15 hybrid cells. (共著)

    Neurochemistry International   Vol. 5 ( 5 ) page: 589-601   1983

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  220. Tryptophan hydroxlase system in brain tissue slices assayed by high-performance liquid chromatography. (共著)

    Neurochemistry International   Vol. 5 ( 5 ) page: 603-609   1983

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  221. Demonstration of tryptophan 5-monooxygenase activity in human brain by high-performance liquid chromatography with fluorometric detection. (共著)

    Biochemistry International   Vol. 2 ( 3 ) page: 295-303   1981

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Books 36

  1. PET/CT for Inflammatory Diseases - Basic Sciences, Typical Cases, and Review Reviewed International journal

    Suzuki H, Sawada M( Role: Contributor ,  Basic Science of PET Imaging for Inflammatory Diseases)

    Springer  2020.1 

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    Total pages:250   Language:English Book type:Scholarly book

  2. アルツハイマー病 発症メカニズムと新規診断法・創薬・治療開発

    澤田 誠, 鈴木弘美( Role: Contributor ,  血管脳関門通過たんぱく質の開発)

    株式会社エヌ・ティ・エス  2018.8 

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    Total pages:460   Language:Japanese Book type:Scholarly book

  3. 脳内マクロファージとミクログリア

    澤田 誠, 鈴木弘美( Role: Joint author)

    南山堂  2018.2  ( ISBN:978--4-525-24851-2

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    Language:Japanese

  4. Family Academy・記憶力向上の秘訣 情報を引き出すトレーニングとは

    澤田 誠( Role: Sole author)

    河合塾  2018.4 

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    Language:Japanese

  5. 文芸春秋SPECIAL夏・脳科学的に正しい「もの忘れ」予防術

    澤田 誠( Role: Sole author)

    文芸春秋社  2017.7 

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    Language:Japanese

  6. 洋泉社MOOK・最強の記憶術

    澤田 誠( Role: Supervisor (editorial))

    洋泉社  2017.2  ( ISBN:978-4-8003-1142-9

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  7. 東京工業大学同窓会誌:質量分析イメージングで脳の機能をどこまで解明できるか?

    澤田 誠( Role: Joint author)

    一般社団法人蔵前工業会  2017 

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    Language:Japanese

  8. 一個人(6月号)・あっ!名前だけが出てこない!!

    澤田 誠( Role: Sole author)

    KKベストセラーズ  2016.6  ( ISBN:115790516

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  9. 子供の科学・キミのハテナを科学するなぜ?なぜ?どうして

    澤田 誠( Role: Sole author)

    誠文堂新光社  2015.3 

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  10. Annual Review 2015 神経・神経炎症におけるミクログリアの多様性

    鈴木則宏、祖父江元、荒木信夫、宇川義一、川原信隆、澤田 誠 ( Role: Joint author)

    中外医学社  2015.1 

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  11. なぜ名前だけがでてこないのか

    澤田 誠( Role: Sole author)

    誠文堂新光社  2013.12  ( ISBN:13:978-4416713

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    Language:Japanese

  12. 子どもの科学:目指せ!記憶の達人

    澤田 誠( Role: Sole author)

    誠文堂新光社  2013.8 

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  13. なぜ人は夢をみるのか? 子供の科学

    澤田 誠( Role: Sole author)

    誠文堂新光社  2012.1 

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  14. CLINICAL NEUROSCIENCE: てんかんとアストロサイト

    澤田 誠( Role: Joint author)

    中外医学社  2011.11 

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  15. Etiology and Pathophysiology of Parkinson's Disease: Role of microglia in inflammatory process, in Parkinson's disease

    Sawada H, Suzuki H, Ono K, Imamaura K, Nagatsu T, Sawada M( Role: Joint author)

    InTech  2011.10  ( ISBN:978-953-307-462-7

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  16. 薬剤学: ミクログリアによるBBB非崩壊型の脳へのターゲティングDDS

    澤田 誠、鈴木弘美、小野健治( Role: Joint author)

    日本薬剤学会  2011 

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  17. 脳循環代謝: 小動物における定量的画像解析

    外山宏、籏野健太郎、鈴木弘美、工藤元、野村昌彦、山田貴史、木村裕一、市瀬正則、澤田誠( Role: Joint author)

    日本脳循環代謝学会  2010.7 

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    Language:Japanese

  18. パーキンソン病-基礎・臨床研究のアップデート-

    澤田 誠( Role: Joint author)

    日本臨牀社  2009.6 

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    Language:Japanese

  19. Frontiers in Parkinson Disease : 家族性パーキンソン病は弧発性パーキンソン病のモデルになるか? 「No」の立場から

    澤田 誠( Role: Joint author)

    メディカルレビュー社  2009.4 

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    Language:Japanese

  20. Protocols for Neural Cell Culture・Isolation of microglia subpopulations

    SAWADA Makoto, SUZUKI Hiromi( Role: Joint author)

    Humana Prerss  2009 

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    Language:English

  21. 老年期痴呆の治療ターゲットとしてのミクログリア

    ( Role: Sole author)

    老年期痴呆研究会誌  2007 

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    Language:Japanese

  22. Molecular pathologies of and enzyme replacement therapies for lysosomal diseases.

    SAKURABA H, SAWADA M, MATSUZAWA F, AIKAWA S, CHIBA Y, JIGAMI Y, ITOH K( Role: Joint author)

    CNS & Neurological Disorders-Drug Targets  2006.8 

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    Language:English

  23. 医学のあゆみ・血液脳関門とDDS

    澤田 誠( Role: Sole author)

    2005.8 

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    Language:Japanese

  24. CLINICAL NEUROSCIENCE 別冊・けいれん発作とグリア細胞の動態

    萩原英雄、澤田 誠( Role: Joint author)

    中外医学社  2005.2 

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    Language:Japanese

  25. 免疫学からみた神経系と神経疾患「共著」

    ( Role: Joint author)

    日本醫學館  1999 

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    Language:Japanese

  26. 現代用語百科 バイオテクノロジー編 第2版

    ( Role: Joint author)

    東京化学同人  1994 

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    Language:Japanese

  27. Tyrosine hydroxylase, tryptophan hydroxylase, biopterin and neopterin in the brain and biopterin and neopterin in sera from patients with Alzheimer's desease. (共著)

    ( Role: Joint author)

    Springer-Verlag  1990 

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    Language:English

  28. Distribution of pterins in biological tissues.

    ( Role: Joint author)

    Elsevier  1987 

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    Language:English

  29. Distribution of GTP cyclohydrolase I, neopterin and biopterin in the human brain. (共著)

    ( Role: Joint author)

    Walter de Gruyter and Co.  1986 

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    Language:English

  30. Abnormality of central monoaminergic neurons in spontaneously hypertensive rats : in vitro and in vivo(volmammetry)studies. (共著)

    ( Role: Joint author)

    Elsevier  1986 

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    Language:English

  31. A new fluoroimmunoassay of biopterin and neopterin in human urine.

    ( Role: Joint author)

    Walter de Gruyter and Co.  1985 

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    Language:English

  32. Biosynthesis of tetrahydrobiopterin in parkinsonian human brain ; activity of GTP cyclohydrolase (]G0001[). (共著)

    ( Role: Joint author)

    Raven Press  1985 

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    Language:English

  33. Effects of tyrosine administration on plasma biopterin in pateints with juvenile parkinsonism and their relatives. (共著)

    ( Role: Joint author)

    Raven Press  1985 

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    Language:English

  34. Elusidation of hydroxylases by calmodulin antagonists. (共著)

    ( Role: Joint author)

    Academic Press  1985 

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    Language:English

  35. Radioimmunoassay for neopterin in body fluids and tissues. (共著)

    ( Role: Joint author)

    Walter de Gruyter and Co.  1983 

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    Language:English

  36. Determination of biopterin and neopterin in fluids and tissues by radioimmunoassay. (共著)

    ( Role: Joint author)

    Walter de Gruyter  1983 

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    Language:English

▼display all

Presentations 165

  1. Signal Peptides as apossible and novel biomarker for extracellular vesicles Invited

    2021.3.28 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  2. シグナルペプチド:細胞外微粒子機能の新規マーカー

    澤田誠

    CREST [細胞外微粒子]第3領域会議  2021.1.7  CREST [細胞外微粒子]領域

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    Event date: 2021.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:web   Country:Japan  

  3. Application of single cell mass spectrometry with hotmelt LMD for understanding neuronal disfunctions

    2020.7.4 

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    Event date: 2020.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  4. オリゴデンドロサイトへ分化誘導したグリア前駆細胞株から放出されるエクソソームの性質

    小野健治, 大橋和哉, 鈴木弘美, 澤田 誠

    第43回日本神経科学大会  2020.7.29  日本神経科学会

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    Event date: 2020.7 - 2020.8

    Language:English   Presentation type:Poster presentation  

    Venue:神戸   Country:Japan  

  5. ホットメルトレーザーマイクロダイセクションおよび並列LCを用いた高速LC-MSイメージングシステムの開発

    古賀裕介, 洪暎淳, 松本龍太, 鈴木弘美, 小野健治, 前田裕樹, 小河潔, 澤田 誠

    第68回質量分析総合討論会  2020.5.11  日本質量分析学会

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    Event date: 2020.5

    Language:Japanese   Presentation type:Poster presentation  

    Venue:大阪 web開催   Country:Japan  

  6. 単一神経細胞の質量分析イメージングでの脳の機能を見る

    澤田 誠

    次世代脳プロジェクト 冬のシンポジウム 

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    Event date: 2018.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  7. Preliminary study on LC-MS/MS analysis of related drugs, nuerotransmitters, translocator protein (TSPO) ligands in brain/liver homogenates by API4000 in a single HILIC system

    Andrea Roxanne J. ANAS, Morosaki Junko, Torii Yuna, Oya Michiko, Suzuki Hiromi, Imanishi Susumu Y, Sawada Makoto, Harada Ken-ichi

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    Event date: 2018.12

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  8. 神経変性疾患に関連する薬剤と神経伝達物質およびL-アミノ酸のLC-MS/MS条件の最適化

    名城大学薬学部環境科学研究室発表会 

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    Event date: 2018.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名城大学薬学部   Country:Japan  

  9. LMD-LC-MSによる脳内の薬物動態と神経伝達物質変化の細胞毎イメージング

    鈴木弘美、Andrea Anas, 小野健治、大石幸一、澤田 誠

    第41回日本分子生物学会年会 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:パシフィコ横浜(横浜市西区みなとみらい)   Country:Japan  

  10. LC-MS/MS analysis of neurotransmitters,translocator protein (TSPO) ligands and related drugs in a single hilic system International conference

    Andrea Roxanne J. ANAS, Morosaki Junko, Torii Yuna, Oya Michiko, SuzukiHiromi, Imanishi Susumu Y, Sawada Makoto, Harada Ken-ichi

    45th Annual convention of the Philipine Society of Biochemistry and Molecular Biology 

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    Event date: 2018.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Philippines  

  11. 記憶とは何か?脳が記憶を作る仕組み

    澤田 誠

    なごや島津会 研修会 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:Hotels & Resorts ISE-SHIMA   Country:Japan  

  12. LC-MS/MSを用いた神経伝達物質、TSPOおよび関連薬物の同時定量

    Andrea Roxanne J. ANAS, 森崎潤子, 鳥居優奈, 大矢倫子, 鈴木弘美, 今西 進, 澤田 誠, 原田健一

    第43回日本医用マススペクトル学会年会 

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    Event date: 2018.9

    Language:English   Presentation type:Poster presentation  

    Venue:北海道大学学術交流会館   Country:Japan  

  13. 質量分析の限界を突破して医療・創薬に応用する前処理装置 Invited

    澤田 誠

    JASIS 2018 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:幕張メッセ   Country:Japan  

  14. 記憶の仕組み、夢の意味 Invited

    澤田 誠

    (公益社団法人)生体制御学会学術集会 市民公開講座 特別公演 

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    Event date: 2018.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋市立大学医学部総合情報センター   Country:Japan  

  15. 「記憶」とは何か〜脳の働きと記憶の仕組み、夢の意味〜

    澤田 誠

    河合塾文化講演会 

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    Event date: 2018.7

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  16. なぜ名前が出てこないのか〜記憶のメカニズムに基づいた名前の覚え方

    澤田 誠

    愛知県高圧ガス協会講演会  

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    Event date: 2018.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:ウィンク愛知   Country:Japan  

  17. Liquid chromatography optimization of pilocarpine, amino acids, neurotransmitters and related drug by API4000 based on log D

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    Event date: 2018.3

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  18. 光感受性陽イオンチャネルChR2を介したNG2陽性グリア前駆細胞のオリゴデンドロサイトへの分化

    小野健治、川嶋裕人、鈴木弘美、澤田誠

    第90回日本生化学会大会(ConBio2017) 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:神戸国際展示場   Country:Japan  

  19. MALDI MSイメージングでの生体高分子の検出やLC-MS/MS分析でも質量イメージングを可能にする技術の確立

    鈴木弘美、Andrea Anas,大矢倫子、小野健治、大石幸一、澤田誠

    第40回日本分子生物学会年会(ConBio2017) 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:神戸国際展示場   Country:Japan  

  20. 「なぜ名前だけが出てこないのか?」〜脳科学者が教える記憶力の鍛え方〜

    澤田 誠

    平成29年度県民公開講座 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:金沢ニューグランドホテル   Country:Japan  

  21. Hot-melt MALDI-MS法によるエクソソーム中のペプチド分析

    橋本洋佑, 澤田誠

    第9回RNAi研究会および第4回日本細胞外小胞学会 

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    Event date: 2017.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:グランドプリンスホテル広島   Country:Japan  

  22. 特殊な情報処理を支える脳の細胞の分析から脳の機能を探る International conference

    澤田 誠

    2017年度星薬科大学薬理学教室卒論研修セミナー 

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    Event date: 2017.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:立山プリンスホテル(長野県大町市平大町温泉郷)   Country:Japan  

  23. LC-MS/MS for neural catecholamines, drugs and TSPO ligands by API4000 International conference

    RACI(Royal Australian Chemical Institute) Centenary Cogress 

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    Event date: 2017.7

    Language:English   Presentation type:Poster presentation  

    Venue:Melbourne,Australia   Country:Australia  

  24. 光感受性イオンチャネルChRGRを介したミクログリアの機能制御

    小野健治、鈴木弘美,今野歩、石塚徹、平井宏和、八尾寛、澤田誠

    第40回日本神経科学大会 

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    Event date: 2017.7

    Language:English   Presentation type:Poster presentation  

    Venue:幕張メッセ   Country:Japan  

  25. トランスミットーム"解析とLC-MS質量イメージング" Invited

    澤田 誠

    コスモバイオ創薬支援セミナー 

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    Event date: 2017.7

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:コングレスクエア日本橋   Country:Japan  

  26. 質量分析イメージングで脳の機能をどこまで解明できるか?

    澤田 誠

    蔵前工業会東海支部総会・講演会 

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    Event date: 2017.6

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:ウィンク愛知   Country:Japan  

  27. LC-MS Imagingによる脳内での薬物動態と神経伝達物質変化の同時解析

    大矢倫子、鈴木弘美、大石幸一、小野健治、澤田誠

    第2回医薬系3部局交流シンポジウム 環境医学研究所・群馬大学生体調節研究所合同シンポジウム 

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    Event date: 2017.6

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋大学 野依記念学術交流館   Country:Japan  

  28. なぜ名前だけが出てこないのか

    澤田 誠

    半田労働基準協会定時会員総会 

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    Event date: 2017.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:住吉福祉文化会館(半田市宮路町)   Country:Japan  

  29. LC-MS Imagingによる脳内での薬物動態と神経伝達物質変化の同時解析

    大矢倫子、鈴木弘美、大石幸一、小野健治、澤田誠

    第65回質量分析総合討論会 

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    Event date: 2017.5

    Language:Japanese   Presentation type:Poster presentation  

    Venue:つくば国際会議場   Country:Japan  

  30. Optimization for Neural Catecholamines, its metabolites and Related Drugs in a Sigle LC-MS/MS Analysis using API4000

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    Event date: 2017.3

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  31. LC-MS対応質量分析イメージング前処理装置の開発 Invited International conference

    澤田 誠

    第16回国際ナノテクノロジー総合展・技術会議 

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    Event date: 2017.2

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:東京ビッグサイト   Country:Japan  

  32. 質量分析を用いた新規な神経系の単一細胞レベルでの安全性評価技術 Invited

    澤田 誠

    第8回日本安全性薬理研究会学術年会 

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    Event date: 2017.2

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:東京大学弥生講堂   Country:Japan  

  33. 名前が思い出せないわけ〜理由がわかれば対処法が変わる〜

    澤田 誠

    北名古屋市生涯学習(3) 

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    Event date: 2017.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北名古屋市健康ドーム   Country:Japan  

  34. ホットメルト質量分析イメージングによる脳細胞活動の測定と薬物動態解析への応用

    澤田 誠

    LSBMセミナー 

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    Event date: 2017.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学先端科学技術研究センター   Country:Japan  

  35. なぜ名前だけがでてこないのか〜記憶のメカニズムに基づいた名前の覚え方

    澤田 誠

    中和医療会総会 

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    Event date: 2017.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  36. 記憶を作るメカニズム〜「睡眠と夢」眠りが記憶を強化する〜

    澤田 誠

    北名古屋市生涯学習(2) 

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    Event date: 2016.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北名古屋市健康ドーム   Country:Japan  

  37. 脳のはたらきと「記憶」の意味〜脳はありのままを見ていない〜

    澤田 誠

    北名古屋市生涯学習(1) 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  38. あれ誰だっけ、名前が出てこない〜記憶の仕組み〜

    澤田 誠

    名古屋物産会定時総会並びに129回例会 

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    Event date: 2016.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:三井物産中部支社   Country:Japan  

  39. 光感受性イオンチャネルChRGRを介したミクログリアの機能調節に関する解析

    小野健治、鈴木弘美、今野 歩、平井宏和、八尾 寛、澤田 誠

    第89回日本生化学会大会in 仙台 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台国際センター   Country:Japan  

  40. グリア前駆細胞株OS3細胞のチャネルロドプシン2を介したオリゴデンドロサイトの分化

    小野健治、滝戸悠平、山本龍生、佐橋秀紀、鈴木弘美、澤田 誠

    第39回日本神経科学会 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Poster presentation  

    Venue:パシフィコ横浜   Country:Japan  

  41. ミクログリアのサブタイプと脳神経疾患における役割

    澤田 誠

    先端医療振興財団 臨床研究センター 研修会 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸市先端医療振興財団   Country:Japan  

  42. 名前が思い出しにくいわけ〜理由がわかれば対処法がわかる〜

    澤田 誠

    生涯学習講座(3) なぜ名前だけがでてこないのか?ー記憶のメカニズム 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岩倉市生涯学習センター   Country:Japan  

  43. 記憶を作るメカニズム〜睡眠と夢〜

    澤田 誠

    生涯学習講座(2) なぜ名前だけがでてこないのか?ー記憶のメカニズム 

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    Event date: 2016.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岩倉市生涯学習センター   Country:Japan  

  44. 3D質量分析イメージングを実現する前処理技術

    澤田誠、鈴木弘美、小野健治、大石幸一

    第64回日本質量分析総合討論会 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Poster presentation  

    Venue:大阪(ホテル阪急エキスポパーク)   Country:Japan  

  45. 脳のはたらきと「記憶」の意味

    澤田 誠

    生涯学習講座(1) 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:岩倉市生涯学習センター   Country:Japan  

  46. なぜ名前だけが出てこないのか~保存より呼び出しが大事だった!正しい記憶の引き出し方~

    澤田 誠

    名古屋市名東区地域力推進総会 

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    Event date: 2016.4

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名東区役所   Country:Japan  

  47. ホットメルト質量分析法による単一細胞レベルの薬物動態と生体機能変化の解析

    澤田 誠

    コスモバイオ講演会 

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    Event date: 2016.4

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:コスモバイオ本社大会議室   Country:Japan  

  48. なぜ名前だけが出てこないのか~保存より呼び出しが大事だった!正しい記憶の引き出し方~

    澤田 誠

    京都自然食品の会 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:京都農協会館   Country:Japan  

  49. ~名古屋大学教授×河合塾トップ講師のコラボ講演~「難関大学 合格への秘訣」

    澤田 誠

    河合塾三重県学生支援プロジェクト 

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    Event date: 2016.2

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:四日市都ホテル   Country:Japan  

  50. ホットメルト質量分析法による単一細胞レベルの薬物動態と生体機能変化の解析

    澤田 誠

    平成27年度名城大学薬学部環境科学研究室発表会 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  51. 光刺激したグリア前駆細胞株OS3ChR2細胞注入による脱髄疾患モデルマウスの運動機能改善

    小野健治、滝戸悠平、山本龍生、佐橋秀紀、鈴木弘美、澤田 誠

    第38回日本分子生物学会年会・第88回日本生化学会大会合同大会 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:神戸ポートアイランド   Country:Japan  

  52. なぜ名前だけが出てこないのか~保存より呼び出しが大事だった!正しい記憶の引き出し方~

    澤田 誠

    半田市講演会 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:半田市役所 大会議室   Country:Japan  

  53. 質量分析イメージングで脳の機能がどこまで見えるか?

    澤田 誠

    未来口腔医療研究センター講演会 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:愛知学院大学院歯学部楠元学舎   Country:Japan  

  54. マクロファージのM1/M2分類の概念でミクログリアの多様性を説明できるか

    澤田 誠

    第13回神経科学研究会 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:大日本住友製薬(株)東京支社   Country:Japan  

  55. めざせ!記憶の達人〜名前、英単語、年号が覚えにくいしくみ〜

    澤田 誠

    河合塾講演会 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:河合塾名古屋校   Country:Japan  

  56. 技術の伝承と知識管理

    澤田 誠

    平成27年度中部地域づくり講演会 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:メルパルク名古屋   Country:Japan  

  57. ホットメルト質量分析イメージングによる脳細胞活動の測定と薬物動態解析への応用

    澤田 誠

    日本生物工学会 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋大学ベンチャービジネスラボラトリー   Country:Japan  

  58. ホットメルト質量分析による機能的バイオマーカーの探索と薬物作用との相関関係

    澤田 誠

    名古屋大学予防早期医療創成センター 第5回ワークショップ 

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    Event date: 2015.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋大学 ES総合館   Country:Japan  

  59. なぜ名前だけが出てこないのかー記憶のメカニズムを知って健康な脳を保つー

    澤田 誠

    第1回シニアライフをデザインする 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋市名東生涯学習センター   Country:Japan  

  60. なぜ名前だけがでてこないのか〜記憶のメカニズムに基づいた名前の覚え方

    澤田 誠

    平成27年度定期総会 特別講演(名古屋南労働基準協会主催 ) 

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    Event date: 2015.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋東急イン('15.04.01より名古屋栄東急REIホテルに改名)   Country:Japan  

  61. 脳標的化薬物デリバリー研究の最前線

    澤田 誠

    日本薬学会第135年会(神戸) 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:神戸学院大学   Country:Japan  

  62. 精神疾患におけるミクログリアサブタイプの関与

    澤田 誠

    第88回日本薬理学会年会(シンポジウム13) 

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    Event date: 2015.3

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋国際会議場   Country:Japan  

  63. 3D Molecular Imaging by LMD-MS International conference

    Sawada Makoto

    PITTCON CONFERENCE & EXPO 2015 

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    Event date: 2015.3

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  64. 質量分析イメージングによる脳細胞活動の測定と薬物動態解析への応用

    澤田 誠

    薬学会東海支部講演会 

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    Event date: 2015.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名城大学   Country:Japan  

  65. LMD-MS: A novel mass spectrometry imaging

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    Event date: 2014.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  66. LMD-MS法:新しい質量分析イメージング技術の確立

    澤田 誠、名古屋大学

    BioJAPAN 2014 

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    Event date: 2014.10

    Language:Japanese  

    Venue:パシフィコ横浜(横浜)   Country:Japan  

  67. ミクログリアのサブタイプと脳神経疾患における役割

    澤田 誠

    創薬薬理フォーラム 第22回シンポジウム 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:パシフィコ横浜   Country:Japan  

  68. グリア細胞は何をしているか?ミクログリアの多様性とニューロン・グリアネットワーク

    澤田 誠

    7th J-CAN(シヌクレイノパチーの病態・治療研究最前線) 

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    Event date: 2014.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  69. Detection of Aβ1-40 monomer/polymers on mouse brain sections by LMD-based MS imaging

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    Event date: 2014.5

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  70. ミクログリアのサブタイプと認知症病態に置ける役割

    澤田 誠

    第32回日本認知症学会学術集会 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:キッセイ文化ホール(長野県松本市)   Country:Japan  

  71. ミクログリアのサブタイプについて レーザーマイクロディセクションと質量分析による検討

    澤田 誠

    第11回神経科学研究会 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:大日本住友製薬東京支社   Country:Japan  

  72. ミクログリアの七変化

    澤田 誠

    第18回日本感染症学会総会学術集会(教育講演) 

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    Event date: 2013.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:フェニックスシーガイアリゾート   Country:Japan  

  73. 高分解能・高空間分解能をもつ3D-in situ 質量分析前処理技術

    澤田 誠

    Bio JAPAN 

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    Event date: 2013.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:横浜みなとみらい   Country:Japan  

  74. ヒトはなぜ夢をみるのか〜脳科学が解き明かす夢と記憶のしくみ〜

    澤田 誠

    河合塾 特別講演 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:河合塾名駅校   Country:Japan  

  75. グリア前駆細胞株OS3のチャネルロドプシン2を介したオリゴデンドロサイトへの分化

    小野健治、滝戸悠平、山本龍生、佐橋秀紀,呉起、鈴木弘美、澤田 誠

    第86回日本生化学会大会 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:パシフィコ横浜   Country:Japan  

  76. Glutamate release from astrocyte cell-line GL261 by photo-activated channelrhodopsin-2

    Ono Kenji, Higa Madoka, Tabata Kaori, Suzuki Hiromi, Sawada Makoto

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    Event date: 2013.6

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  77. ものづくりの脳と夢を見る仕組み

    澤田 誠

    第10回NPOものづくり中部総会並びに記念講演会 

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    Event date: 2013.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:ルブラ王山(名古屋)   Country:Japan  

  78. 光操作によるミクログリアの活性化

    澤田 誠

    第90回日本生理学会大会 

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    Event date: 2013.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:東京   Country:Japan  

  79. ミクログリアのサブタイプ 毒性転換と脳機能発現の関わり

    澤田 誠

    第29回Neuroscience Seminar Tokushima 

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    Event date: 2013.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:徳島大学   Country:Japan  

  80. 生体機能のイメージングプローブの開発

    澤田 誠

    名古屋大学予防早期医療創成センタ-第3回ワークショップ 

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    Event date: 2013.1

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋大学理学南館理学セミナー室   Country:Japan  

  81. チャネルロドプシン2を導入したOS3グリア前駆細胞株における細胞分化の光制御

    小野健治、山本龍生、佐橋秀紀、鈴木弘美、澤田 誠

    第85回日本生化学会大会 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:福岡国際会議場   Country:Japan  

  82. ミクログリアのサブタイプによる高次脳機能制御の可能性

    澤田 誠

    愛媛大学大学院フォーラム 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:愛媛大学   Country:Japan  

  83. 夢をみる仕組み〜脳科学からみたよい夢をみるヒント〜

    澤田 誠

    名古屋市民大学講座 

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    Event date: 2012.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:愛知県産業労働センタ-   Country:Japan  

  84. ミクログリアが関与する脳機能発現のメカニズム解明

    澤田 誠

    ライフサイエンス領域での講演会 

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    Event date: 2012.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:(株)東レ 医薬研究所   Country:Japan  

  85. ChR2を導入した神経系前駆細胞株OS3細胞における細胞分化の光制御

    小野健治、山本龍生、佐橋秀紀、鈴木弘美、澤田 誠

    第35回日本神経科学大会 

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    Event date: 2012.9 - 2019.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋国際会議場   Country:Japan  

  86. ミクログリアのサブタイプと毒性転換について

    澤田 誠

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    Event date: 2012.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ベルサール八重洲(東京)   Country:Japan  

  87. ヒトはなぜ夢をみるのか?脳科学が解き明かす夢のしくみ

    澤田 誠

    河合塾2012文化行事 

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    Event date: 2012.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:河合塾名駅キャンパス   Country:Japan  

  88. 光機能制御グリア細胞を用いた神経修復・髄鞘再生戦略

    澤田 誠

    第53回日本神経学会学術大会 

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    Event date: 2012.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京国際フォーラム   Country:Japan  

  89. 血液脳関門を壊さない安全性の高い脳標的化技術

    澤田 誠

    第13回名古屋大学 大学発・より抜きバイオセミナー 

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    Event date: 2012.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:バイオインダストリー協会(JBA)(東京中央区)   Country:Japan  

  90. ミクログリアのサブタイプと毒性転換

    澤田 誠

    愛知県心身障害者コロニー発達障害研究所公開セミナー 

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    Event date: 2012.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:心身障害者コロニー(春日井)   Country:Japan  

  91. ヒトはなぜ夢をみるのか?

    澤田 誠

    第19回名大カフェSCIENCE,and Me 

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    Event date: 2012.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  92. 光を使ってミクログリアの毒性転換をコントロールする

    澤田 誠

    第8回群馬大学名古屋大学合同シンポジウム 

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    Event date: 2011.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:群馬大学生体調節研究所   Country:Japan  

  93. 光を使ってミクログリアの毒性転換を調節する

    澤田 誠

    第9回神経科学研究会 

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    Event date: 2011.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大日本住友製薬株式会社 東京支社   Country:Japan  

  94. 神経疾患モデルにおけるミクログリアの毒性転換と脳機能の画像化の試み

    澤田 誠

    アステラス製薬バイオイメージング研究所・薬理研究所合同セミナー 

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    Event date: 2011.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:アステラスバイオイメージング研究所   Country:Japan  

  95. 活性化に伴いiNOSを発現する細胞をMRIで可視化する方法の開発

    小野健治、田畑香織、鈴木弘美、澤田誠

    第34回日本神経科学大会 

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    Event date: 2011.9

    Language:English   Presentation type:Oral presentation (general)  

    Venue:パシフィコ横浜   Country:Japan  

  96. ミクログリアの毒性転換のイメージングとサブタイプ

    澤田 誠

    ミクログリア研究会 

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    Event date: 2011.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:アスビオファーマ本社(神戸)   Country:Japan  

  97. 細胞を使った脳の標的化技術と脳の機能イメージング

    澤田 誠

    第19回薬理学セミナー 

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    Event date: 2011.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:愛知学院大学(楠元学舎)   Country:Japan  

  98. 浸透圧性脱髄ラットモデルにおける脱髄病変内ミクログリアの遺伝子発現プロファイル

    鈴木陽之、椙村益久、岩間信太郎、清田敦、鈴木弘美、長崎弘、有馬寛、澤田 誠、大磯ユタカ                 

    第84回日本内分泌学会学術総会 

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    Event date: 2011.4

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸国際会議場(神戸)   Country:Japan  

  99. 毒性転換したミクログリアのin vivoイメージングと脳の標的化DDS技術

    澤田 誠

    第16回創剤フォーラム若手研究会 ー 次の十年につながる材料・製剤・DDS技術 

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    Event date: 2010.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  100. Control of activation in ChR2-expressing astrocytes by blue light exposure

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    Event date: 2010.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  101. Comparative analysis of gene expression profiles of microglia from demyelinative lesions in osmotic demyelination syndrome rat model International conference

    10th International Congress of Neuroimmunology 

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    Event date: 2010.10

    Language:English   Presentation type:Poster presentation  

  102. Control of activation in ChR2-expressing astrocytes by blue light exposure

    第53回日本神経化学会(神戸)大会 

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    Event date: 2010.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  103. ミクログリアの毒性転換のin vivoイメージングと光技術による活性化の制御

    澤田 誠

    トレリーフ錠発売1周年記念講演会 

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    Event date: 2010.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  104. ミクログリアの毒性転換のIn Vivoイメージングと脳の標的化DDS技術

    澤田 誠

    第3回アルツハイマー病の早期診断・治療・予防法の開発研究会 

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    Event date: 2009.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  105. Interleukin-4-induced selective clearance of oligomeric beta-amyloid peptide 1-42 by rat primay type 2 microglia

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    Event date: 2009.10

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  106. Visualization of gene expression in magnetic resonance imaging

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    Event date: 2009.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  107. Protective effects of minocycline on osmotic demyelination syndrome in rats International conference

    Neuroscience 2009 

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    Event date: 2009.10

    Language:English   Presentation type:Poster presentation  

  108. ミクログリアの毒性転換のIn vivo イメージング

    澤田 誠

    第7回神経科学研究会 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  109. The differing pathological effects of nitric oxide synthase isoforms in osmotic demyelination syndrome in rats

    The 32nd Annual Meeting of the Japan Neuroscience Society 

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    Event date: 2009.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  110. Neuroprotective or nerotoxic phenotypes of activated microglia in neonatal mice in neuronal injuries

    The 32nd Annual Meeting of the Japan Neuroscience Society 

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    Event date: 2009.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  111. Protective roles of brain-migrated immature bone marrow cells against NO-dependent neurotoxicity in a brain injury

    The 32nd Annual Meeting of the Japan Neuroscience Society 

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    Event date: 2009.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  112. Minocycline prevents osmotic demyelination by inhibiting the activation of microglia in rats. International conference

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    Event date: 2009.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  113. SIADHモデルラットにおいてミノサイクリンは浸透圧性脱随の発症・進展を防止する

    鈴木陽之、椙村益久、岩間信太郎、鈴木弘美、有馬 寛、澤田 誠、大磯ユタカ

    第36回日本神経内分泌学会学術集会 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  114. シンクロトロン光を用いた細胞制御脳研究への道

    澤田 誠

    第16回[先端技術と原子力]シンポジウム 

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    Event date: 2009.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  115. A practical preparation of [18F]FEPPA using a protic solvent system International conference

    18th International Symposium on Radiopharmaceutical Sciencnes 

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    Event date: 2009.7

    Language:English   Presentation type:Poster presentation  

  116. Enhancement of glioma formation and growth by brain-migrated immature bone marrow cells

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    Event date: 2008.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  117. 磁性微粒子による脳票的化細胞の分離と体内動態イメージングおよび機能解析

    澤田 誠

    第28回ナノバイオ磁気工学研究会 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  118. 大学発ベンチャーの挑戦~今、バイオベンチャーが死の谷をこえられるか?

    澤田 誠

    蔵前工業会東海支部講演会 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  119. ミクログリアの毒性転換のin vivoイメージングと脳の標的化DDS技術

    澤田 誠

    第39回中部化学関係学協会支部連合秋季大会 

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    Event date: 2008.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  120. The expression of proinflammatory cytokines and neurotrophins in glia in osmotic demyelination syndrom International conference

    Society for Neuroscience 

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    Event date: 2008.11

    Language:English   Presentation type:Oral presentation (general)  

  121. ミクログリアイメージングの意義-基礎から臨床へ-

    第24回ブレイン・ファンクション・イメージング・カンファレンス 

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    Event date: 2008.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  122. 脳損傷モデルマウスにおける脳移行性骨髄細胞の神経保護効果

    小野健治、山本奈穂、鈴木弘美、佐藤愛美、澤田 誠

    第51回神経化学会大会 

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    Event date: 2008.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  123. 脳移行性骨髄細胞の脳腫瘍形成・増殖に対する促進的関与

    小野健治、古川大記、鈴木弘美、佐藤愛美、澤田 誠

    第31回神経科学学会 

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    Event date: 2008.7

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  124. 血液脳関門を壊さない脳の標的化DDS技術

    澤田 誠

    第16回群馬遺伝子診療研究会 

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    Event date: 2008.6

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  125. 新規抹消性ベンゾジアゼピン受容体製剤:{18F}FEPPA PETとパーキンソン病モデルラットを用いた活性化ミクログリアのイメージング

    鈴木弘美、外山 宏、籏野健太郎、工藤 元、伊藤文隆、小野健治、加藤隆司、Alan Wilson, 伊藤健吾、市瀬正則、澤田 誠

    第3回日本分子イメージング学会 

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    Event date: 2008.5

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  126. 血管脳関門を壊さない脳標的化技術:脳疾患の新規治療

    澤田 誠

    医薬ライセンシング協会 月例会 第200回記念講演会 

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    Event date: 2008.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  127. 浸透圧性脱髄症候群ラットモデルにおけるグリア細胞の各種サイトカイン及び神経栄養因子の発現解析の検討

    椙村益久、岩間信太郎、鈴木弘美、有馬 寛、高岸芳子、村田善晴、澤田 誠、大磯ユタカ

    第81回日本内分泌学会学術総会 

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    Event date: 2008.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  128. Expression of Cytokines and Neurotrophins after acute Subarachnoid Hemorrhage. International conference

    2008 International Stroke Conference 

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    Event date: 2008.2

    Language:English   Presentation type:Poster presentation  

  129. 脳損傷モデルマウスにおける脳移行性骨髄細胞の活性化と分化に関する解析

    小野健治、山本奈穂、鈴木弘美、佐藤愛美、神澤孝夫、澤田 誠

    第30回日本分子生物学会年会、第80回日本生化学会大会合同大会 

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    Event date: 2007.12

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  130. 血液脳関門を壊さない脳標的化ドラッグデリバリー

    群馬大学生体調節研究所シンポジウム 

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    Event date: 2007.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  131. Neuroprotective and toxic change of microglia in neurodegenerative disease International conference

    3rd International Symposium on Dopaminergic and Nondopaminergic Mechanisms in Parkinson's Disease(INPD) 

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    Event date: 2007.11

    Language:English   Presentation type:Oral presentation (invited, special)  

  132. 動物PETによるラット線条体障害モデルにおけるミクログリアの毒性転換の検討

    鈴木弘美、外山 宏、籏野健太郎、工藤 元、伊藤文隆、小野健治、加藤隆司、伊藤健吾、澤田 誠

    第12回グリア研究会 

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    Event date: 2007.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  133. 動物PETによるラット線条体障害モデルにおけるミクログリアの毒性転換の検討

    鈴木弘美、外山 宏、籏野健太郎、工藤 元、伊藤文隆、小野健治、加藤隆司、伊藤健吾、澤田 誠

    第16回日本バイオイメージング学会学術集会 

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    Event date: 2007.10

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  134. 脳損傷モデルマウスにおける脳移行性骨髄細胞と分化のイメージング.

    小野健治、山本奈穂、鈴木弘美、神澤孝夫、澤田 誠

    第50回日本神経化学(横浜)大会、第30回日本神経科学大会、第17回日本神経回路学会大会合同大会 

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    Event date: 2007.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  135. 第2のプログラム細胞死におけるオートファジーの役割

    神澤孝夫、澤田 誠

    第5回神経科学研究会 

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    Event date: 2007.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  136. 動物PETによるラット線条体障害モデルにおけるミクログリア毒性転換の検討

    伊藤文隆、工藤 元、外山 宏、鈴木弘美、籏野健太郎、加藤隆司、片田和広、市瀬正則、澤田 誠、伊藤健吾

    日本分子イメージング学会第2回総会・学術集会 

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    Event date: 2007.6

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  137. 脳腫瘍とオートファジーについて

    澤田 誠

    第12回鶴舞公開セミナー 

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    Event date: 2007.4

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  138. microgliaの起源, 疾患との関連

    澤田 誠

    第112回日本解剖学会総会・全国学術集会 

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    Event date: 2007.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  139. 脳まで届く薬の秘密 ~脳の病気を安全に狙い撃ちするには~

    澤田 誠

    名古屋大学 健康長寿シーズ発表会 

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    Event date: 2007.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  140. 血液脳関門を壊さない脳標的化ドラッグデリバリーとトリプルターゲッティング

    澤田 誠

    群馬大学生体調節研究所・環境医学研究所合同シンポジウム(第3回) 

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    Event date: 2006.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  141. ミクログリアイメージングの意義

    澤田 誠

    第18回日本脳循環代謝学会総会 

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    Event date: 2006.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  142. Control of microglia neurotoxicity via β- adrenergic receptors

    小野健治, 佐藤愛美, 澤田 誠

    第49回神経化学会大会 

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    Event date: 2006.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  143. Imaging of activated microglia in brain injury

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    Event date: 2006.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  144. ミクログリアに関する研究発表

    澤田 誠

    武田薬品 

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    Event date: 2006.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  145. よいミクログリアと悪いミクログリア

    第29回日本神経科学大会 

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    Event date: 2006.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  146. 乳酸アシド-シス環境におけるマイクログリアのプロトンチャネル活性化.

    久野みゆき, 森畑宏一, 川脇順子, 翁 昌子, 澤田 誠, 酒井 啓

    第29回日本神経科学大会 

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    Event date: 2006.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  147. ミクログリアの活性化がMPTP投与による黒質線条体ドーパミン神経細胞に及ぼす影響は新生児マウスと老齢マウスとは異なる

    澤田浩秀, 菱田良平, 平田洋子, 小野健治, 鈴木弘美, 村松慎一, 中野今治, 土田邦博, 永津俊治, 澤田 誠

    第29回日本神経科学大会 

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    Event date: 2006.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  148. Activated microglia affect the nigro-striatal dopamine neurons differently in neonatal and aged mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine International conference

    20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress 

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    Event date: 2006.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  149. Clearance of Polysialic acid during LPS-induced activation on microglia cells. International conference

    20th IUBMB Inter-national Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress 

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    Event date: 2006.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  150. 1.5T MRIによる定位的ラット脳線条体撮像-定位脳固定具を用いない撮像法の開発

    伊藤文隆, 工藤 元, 外山 宏, 鈴木弘美, 旗野健太郎, 中根正人, 大橋正夫, 加藤隆司, 片田和広, 市瀬正則, 澤田 誠, 伊藤健吾

    日本分子イメージング学会設立総会及び第1回学術集会. 

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    Event date: 2006.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  151. 脳特異的な薬物・遺伝子導入技術の開発

    澤田 誠

    第11回鶴舞公開セミナー 

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    Event date: 2006.4

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  152. 血液脳関門を壊さない脳標的化薬物送達の手法

    澤田 誠

    第15回東海ニューロサイエンス研究会 

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    Event date: 2006.4

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  153. 血液脳関門を壊さない脳標的化ドラッグデリバリー

    澤田 誠

    第18回神経免疫学会 

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    Event date: 2006.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  154. 細胞機能を活用する医療技術の開発:神経疾患のトリプルターゲッティング

    澤田 誠

    戦略ワークショッップ『健康分野』 

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    Event date: 2006.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  155. 血液脳関門を壊さない標的医療

    澤田 誠

    名古屋内科医会例会 

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    Event date: 2006.2

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  156. 脳特異的な薬物・遺伝子・タンパク質導入技術の開発 International conference

    澤田 誠

    環境医学研究所国際シンポジウム 

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    Event date: 2005.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  157. 末梢性ベンゾジアゼピン受容体製剤11C-CB148と11C-PK11195の比較―動物PETによる検討

    関亦克彦,籏野健太郎,外山 宏,鈴木弘美,加藤隆司,片田和廣,澤田 誠, 伊藤健吾

    第45回日本核医学会総会 

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    Event date: 2005.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  158. 活性型ミクログリアのIn Vivoイメージング

    鈴木弘美, 外山 宏, 工藤 元, 籏野健太郎, 関亦克彦, 小野健治, 中根正人, 加藤隆司, 伊藤健吾, 澤田 誠

    第14回日本バイオイメージング学会学術集会 

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    Event date: 2005.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  159. ミクログリアの神経保護作用と毒性転換

    澤田 誠

    第3回神経科学研究会 

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    Event date: 2005.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  160. βアドレナリン受容体を介したミクログリアの神経細胞に対する機能に関する解析

    小野健治、澤田 誠

    第9回神経伝達物質研究会 

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    Event date: 2005.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  161. HIV-Nefを導入したミクログリア細胞による神経細胞機能障害

    澤田 誠,三井健一,鈴木弘美,小野健治,Karl-Heinz Krause,鈴木和男

    第16回日本生体防御学術集会 

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    Event date: 2005.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  162. 末梢性ベンゾジアゼピン受容体製剤11C-CB148と11C-PK11195の比較―動物PETによる検討

    工藤元,関亦克彦,籏野健太郎、外山宏,鈴木弘美,加藤隆司,片田和廣,澤田 誠,伊藤健吾

    核医学会中部地方会 

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    Event date: 2005.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  163. 細胞の性質を使って脳の疾患を治療する試み - ミクログリアの新規な性質と脳票的化技術

    澤田 誠

    第27回神経懇話会 

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    Event date: 2005.6

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  164. 活性型ミクログリアのIn Vivoイメージング

    鈴木弘美, 外山宏, 工藤元, 旗野健太郎, 関亦克彦,小野健治, 加藤隆司, 伊藤健吾,澤田 誠

    第9回ニューロイメージングカンファランス 

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    Event date: 2005.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  165. 人に優しい脳疾患治療法開発の試み アルツハイマー病―その病態と克服へ向けた試み

    澤田 誠

    平成17年度市民公開講座 

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    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

▼display all

Research Project for Joint Research, Competitive Funding, etc. 2

  1. LC-MS対応質量分析イメージング前処理装置の開発

    2014.12 - 2018.3

    先端計測(最先端領域・機器開発) 

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    Grant type:Competitive

  2. PETを用いた痙攣のイメージングバイオマーカー探索

    2013.4 - 2015.3

    出資金による受託研究 

KAKENHI (Grants-in-Aid for Scientific Research) 4

  1. ストレスによる認知情動変容を担う多階層プロセスと精神疾患への関与の構成的理解

    Grant number:18H05429  2018.6 - 2023.3

    文部科学省  科学研究費補助金  新学術領域研究(研究領域提案型)

    古屋敷 智之

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

    社会環境から受けるストレスは、短期的には適応反応を引き起こすが、長期的には生体恒常性の破綻をきたし、精神疾患のリスク因子となる。しかし、ストレス感受性制御を司る個体―回路―細胞―分子の階層間を繋ぐ因果律は不明である。本研究は、ストレス刺激による各脳領域・細胞種での多階層分子変容を同定し、神経回路の機能・形態変化をシナプスから局所・広域回路に亘り可視化し、各種神経細胞培養系も駆使し、ストレス感受性制御のin silico モデルを創成する。分子・光・化学遺伝学的操作によりin silicoモデルの妥当性とストレス感受性との関連を示し、ストレス感受性操作・正常化を試みる。この戦略により、精神疾患病態を担う因果律の同定と正常化のための手法の開発を目指す。
    本年度は、社会挫折ストレスやサイトカイン投与により誘導したストレス感受性モデル動物で、レーザーマイクロダイセクション(LMD)やセルソーターを用い、mPFCなどストレス関連脳領域から細胞種選択的なエピゲノム・トランスクリプトームや組織全体のメタボロームを調べ、関与する転写・エピゲノム制御因子やシグナル伝達分子の候補を同定した。上記のストレス感受性モデルで、三次元電顕、神経活動の組織学的マッピング、多点ECoG電極記録などを用い、神経細胞の微細構造変化やシナプス・回路変容を同定した。膨潤法を改良した生体分子保持型拡張膨潤法を確立し、局所微細構造を特定の染色法で同定することに成功した。また健常者ミクログリア様細胞を立ち上げ、自然免疫受容体刺激による細胞内シグナル伝達変化を確かめた。
    本年度は、ストレス感受性モデル動物でミクログリア活性化に伴うエピゲノム・トランスクリプトーム・メタボローム解析を実施し、階層縦断的因果律解明の起点となる転写・エピゲノム制御因子やシグナル伝達分子の候補を同定できた。ストレス感受性モデル動物で神経細胞の微細構造変化を同定し、トランスクリプトーム・メタボローム解析と統合して、神経細胞の微細構造変化に関わる分子経路を推定し、分子操作技術を開発する手がかりを得た。ストレス感受性モデルでシナプス・回路変容を同定し、ストレス感受性を制御する神経回路を光・化学遺伝学的操作で解明するための手がかりを得た。生体分子保持型拡張膨潤法を開発し、ストレス感受性モデルの脳内の細胞生物学的変化とLMDによる質量分析解析を統合することが可能になった。健常者のミクログリア様細胞を立ち上げ、自然免疫受容体シグナル伝達を確認したことで、マルチオミクスデータ収集のための条件を整えた。以上の研究成果から、本研究はおおむね順調に進展していると言える。
    今後は、ストレス感受性モデルで同定したミクログリア活性化に関わる転写・エピゲノム制御因子やシグナル伝達分子の候補について、遺伝子発現制御や阻害薬を用い、当該因子がストレスによる神経・グリア細胞や行動の変化に関与するかを調べる。ストレス感受性モデルでの神経細胞の微細構造変化をさらに調べ、この変化を操作する分子操作技術を開発し、神経・シナプス活動や情動変容との因果関係に迫る。ストレス感受性モデルでのシナプス・回路変容をさらに調べ、またストレス抵抗性を担う脳領域の入力脳領域や周辺神経回路の変容も調べる。これらのシナプス・回路変容を光・化学遺伝学的に操作し、ストレスに関連した行動・脳活動変化との関係性を検討する。独自に改良した生体分子保持型拡張膨潤法を用い、ストレス感受性モデルの脳内の細胞生物学的変化を観察しつつ、LMDを組み合わせた微細領域の質量分析を行う。健常者ミクログリア様細胞の自然免疫受容体を刺激し、各種オミクス解析を進め、トランスオミクス解析のための準備を進める。

  2. マルチスケール精神病態の構成的理解(総括班)

    Grant number:18H05428  2018.6 - 2023.3

    文部科学省  科学研究費補助金  新学術領域研究(研究領域提案型)

    高木朗子

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\1300000 ( Direct Cost: \1000000 、 Indirect Cost:\300000 )

    本領域の究極の目的は、精神疾患研究に特化した新しい学問と呼ぶにふさわしいサステイナブルな学術フォーラムを作ることである。そのため、総括班として、計画班および公募班の有機的な連携を促し、領域全体の研究力の底上げおよび、領域活動についての広報活動を行った。【研究支援】総括班・研究支援委員会では、技術や生物試料(iPS細胞、死後脳など)の供与などを介して、領域全体へ支援活動を行った。その結果、領域発足以降2年弱における領域内共同研究は、ゲノム支援(8件)、iPS細胞支援(2件)、行動解析支援(6件)、1細胞支援(1件)、イメージング支援(7件)、その他研究支援(41件)の合計68件にのぼり、打ち合わせ中(8件)、実験進行中(37件)、論文投稿準備中(9件)、論文投稿中(5件)という進捗具合である。【国際研究活動支援】領域活動の国際周知のために、第1回国際シンポジウムを、日本神経科学大会の前日に行った(2019年7月24日)。8名の演者は、計画班員4名、国際招聘4名(Hailan Hu、Zhen Yan、Pierre Vanderhaeghen、Rainbo Hultman)で構成され、100名近い参加者による白熱したシンポジウムとなった。また、若手研究者を中心とする領域研究者4名が、コーネル大学、ハーバード大学、セゲド大学、オックスフォード大学へ短期(~1か月)派遣された。【領域会議・若手育成合宿】第2回伊豆山研修センター(2019年8月31日~9月1日)、第1回若手育成合宿(2019年8月30日、松沢病院)を開催した。【次世代脳】脳科学関係の新学術領域の集合からなる2大規模学術集会「次世代脳」に参画し(2019年12月19日)、「3領域合同若手シンポジウム」を主催した。【ニュースレター】1回/年刊行した。
    総括班として当初予定していたすべてのイベント・活動(領域会議、若手育成合宿、国際シンポジウム、次世代脳、ニュースレター、研究支援)を予定通りに遂行できた。シンポジウムなどの行事参加人数も多く、議論も活発に行われた。シニアから若手研究者まで満足できる質を担保できているとの多くの肯定的な意見も頂いている。学術調査官や領域外アドバザーの先生方からも同様である。研究支援活動に関しても、十分な数の領域内共同研究がはじまり、その多くが内実の伴った共同研究として発展している。とりわけ異種グループ(A1グループ、A2グループ、A3グループと、3つあるグループで、異なるグループ間)での共同研究が多く進行し、これは領域の理念が浸透したためであると考えられる。
    これまでの領域の活動に関しては、COVID-19の影響を除いて、問題は生じていないため、この点以外はこれまでと同様の戦略で進めていく。
    【COVID-19】コロナ禍により影響を受ける活動として、まず第2回若手育成合宿、第3回領域会議およびがあげられ、これは本来、2020年7月2~4日の日程で開催する予定だったが、すべてWEB配信(Zoom会議)で行う。また新学術領域と他の大型グラントとの差別化の一つとして、より強い領域内の連携が必要と考える。そのため、懇親会に関しても、オンラインで遂行するが、そのために、Zoomを20程度の別々のセッションへ分割し、その各々にメインとなるPIを配置する。欧米のシンポジウムで時折行われる、Meet-The-Expert Sessionのように、若手が普段なかなか聞きにくいことを自由に相談できる場にし、オンラインの良さを生かした運営を目指す。第2回若手育成合宿に関しては、2020年12月に「神経回路の可視化・操作・モデリングのための最先端技術」を開催する予定である。講演内容は、昨今の最先端技術のノウハウを若手研究者に対する対話式講演として行い、その内容は、三次元電顕・コネクトーム(講師・敬称略:永井裕崇)、神経回路の数理モデリング(豊泉太郎)、in vivo電気生理(水関健司)、ゲノム編集(三國貴康)、脳透明化(田井中一貴)、single cell RNA-seq(増田隆博)の内諾を得ている。万が一、COVID-19の事象が看過できない状態だとしたら、オンラインでの開催を検討する。次世代脳(2020年12月16日)への参画に関しては、これまでと同様に、「脳構築の時計と場(影山代表)」「スクラップビルド(榎本代表)」と3領域合同若手シンポジウムを開催する予定であるが、諸事情を勘案して最終判断する。

  3. 脳移行性ペプチドを用いた新規脳機能イメージング用PETリガンドの開発      

    2008.4 - 2010.3

    科学研究費補助金  基盤研究(B)

    澤田 誠

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    Authorship:Principal investigator 

  4. 代謝的ポテンシャル依存的化学ゆらぎによるミクログリアの毒性/保護性の変動    

    2008.4 - 2009.3

    科学研究費補助金  萌芽研究,課題番号:20650055

    澤田 誠

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    Authorship:Principal investigator 

Industrial property rights 15

  1. 質量分析方法

    澤田誠、小野健治、鈴木弘美、王勇、緒方是嗣、村田匡

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    Applicant:東海国立大学機構、JSR、島津製作所

    Application no:2020-208582  Date applied:2020.12

  2. レーザマイクロダイセクション装置

    澤田誠、鈴木弘美、小野健治、洪暎淳

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    Applicant:東海国立大学機構, 島津製作所

    Application no:PCT/JP2020/011793  Date applied:2020.3

  3. 蛍光プローブ及びその製造方法

    小野健治、澤田誠、渕真吾、竹田美和

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    Application no:特願2011-160261  Date applied:2013.1

    Announcement no:WO2013/011984 A1 

    Country of applicant:Domestic  

  4. 蛍光プローブ及びその製造方法

    小野健治、澤田誠、渕真吾、竹田美和

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    Applicant:国立大学法人名古屋大学

    Application no:特願2011-160261  Date applied:2011.7

    Country of applicant:Domestic  

  5. 多価結合手を有し代謝安定性が向上した脳移行性ポリペプチド

    中條智洋、原啓高、山本一匡、鈴木弘美、澤田誠、須原哲也、樋口真人、原田平輝志、季斌

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    Applicant:プロテウスサイエンス、独立行政法人放射線医学総合研究所

    Application no:特願2007-054260  Date applied:2007.3

    Country of applicant:Domestic  

  6. Brain-migrating cells, and use thereof

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    Application no:PCT/JP2006/316673  Date applied:2006.8

    Announcement no:WO 2007/023930 

    Country of applicant:Domestic  

  7. Brain disposition marrow progenitor

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    Application no:PCT/JP2005/018785  Date applied:2005.10

    Announcement no:WO 2006/041088 

    Country of applicant:Domestic  

  8. Carrier for migration into cerebral neuron containing metal colloid particle

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    Application no:PCT/JP2005/001761  Date applied:2005.2

    Announcement no:WO 2006/013650 

    Country of applicant:Domestic  

  9. 脳移行活性を有するポリペプチド、およびその利用

    澤田誠

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    Applicant:株式会社 ティッシュターゲティングジャパン

    Application no:PCT/JP2004/011668  Date applied:2004.8

    Announcement no:W0 2005/014625 A1 

    Country of applicant:Foreign country  

    脳移行性を有する複数のポリペプチド取得と共通配列に関する発明とその利用法について

  10. 脳移行活性を有するポリペプチド、およびその利用

    澤田誠

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    Applicant:プロテウスサイエンス株式会社

    Date applied:2004.8

    Patent/Registration no:ZL200480029461.6  Date registered:2009.2 

    Country of applicant:Foreign country  

  11. 株化ミクログリア

    澤田 誠

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    Date applied:1998.3

    Patent/Registration no:第4387108号  Date registered:2009.10 

    Country of applicant:Domestic  

  12. *ミクログリアからなる医用キャリアー

    澤田誠

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    Applicant:科学技術振興機構

    Patent/Registration no:3410738  Date registered:2003.3 

    Country of applicant:Domestic  

    血流中に注入すると血液脳関門を崩壊せずに脳に侵入する能力を有するミクログリア細胞の性質と医療担体としての応用に関する特許

  13. microglial cell line

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    Patent/Registration no:US 6,673,605  Date registered:2004.1 

    Country of applicant:Foreign country  

  14. microglial cell line

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    Patent/Registration no:EP 0949331  Date registered:2007.1 

    Country of applicant:Domestic  

  15. 脳移行活性を有するポリペプチド、およびその利用

    澤田 誠

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    Patent/Registration no:特許第4806258  Date registered:2011.8 

    Country of applicant:Domestic  

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Teaching Experience (On-campus) 7

  1. 薬物動態解析学セミナー

    2020

  2. 基礎医学セミナー

    2020

  3. ベーシックトレーニング

    2020

  4. 薬物動態解析学実験研究

    2020

  5. ナノバイオ計測化学

    2019

  6. 基盤医学特論・特徴あるプログラム「医薬統合プログラム」

    2016

     詳細を見る

    タイトル:質量分析イメージングによる脳細胞活動の測定と薬物動態解析への応用

  7. 基盤医学特論

    2015

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    質量分析イメージングによる脳細胞活動の測定と薬物動態解析への応用

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Teaching Experience (Off-campus) 8

  1. 生命科学部 生命科学特論A

    2019.4 - 2020.3 Hiroshima University)

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    Level:Undergraduate (specialized) 

  2. 生命工学特論 IV

    2017.4 - 2018.3 Gifu University)

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    Level:Postgraduate 

  3. メディカルバイオ特論1

    2014.4 - 2015.3 Nagasaki University)

  4. 口腔機能分子科学

    2014.4 - 2015.3 Kyushu University)

  5. HAM病理組織質量分析法の研究指導+ミクログリアのサブタイプと毒性転換

    2012.4 - 2013.3 Kagoshima University)

  6. 抗酸化タンパクとミクログリアの毒性転換

    2012.4 - 2013.3 Yamagata University)

  7. ミクログリアのサブタイプによる高次脳機能制御の可能性

    2012.4 - 2013.3 Ehime University)

  8. 生命分子薬学特論

    2007.4 - 2008.3 名古屋市立大学薬学研究科)

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Media Coverage 6

  1. スイッチ!シリーズ企画「クイズで解明!脳力ゼミナール」第5回:「引き出す力」 TV or radio program

    東海テレビ  スイッチ!  2021.2

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    Author:Myself 

  2. スイッチ!シリーズ企画「クイズで解明!脳力ゼミナール」第4回:「連想する力」 TV or radio program

    東海テレビ  スイッチ!  2020.11

  3. スイッチ!シリーズ企画「クイズで解明!脳力ゼミナール」第3回:「イメージする力」 TV or radio program

    東海テレビ  スイッチ!  2020.9

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    Author:Myself 

  4. スイッチ!シリーズ企画「クイズで解明!脳力ゼミナール」第2回:「整理する力」 TV or radio program

    東海テレビ  スイッチ!  2020.6

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    Author:Myself 

  5. スイッチ!シリーズ企画「クイズで解明!脳力ゼミナール」第1回: 「引き出す力」 TV or radio program

    東海テレビ  スイッチ!  2020.4

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    Author:Myself 

  6. 脳へ薬届ける抗体創出 Newspaper, magazine

    愛媛新聞  愛媛新聞  2018.6

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    Author:Other 

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