研究者詳細 - 中西　洋一

論文 - 中西　洋一

分割表示 29 件中 1 - 20 件目／全件表示 >>

Letter to the Editor: Speedy Plant Genotyping by SDS-Tolerant Cyclodextrin-PCR 査読有り

Nakanishi, Y; Kawashima, T; Naganawa, M; Mikami, T; Maeshima, M; Ishiguro, S

PLANT AND CELL PHYSIOLOGY 63 巻 ( 8 ) 頁： 1025 - 1028 2022年8月

Conformational Change of the Hairpin-like-structured Robo2 Ectodomain Allows NELL1/2 Binding 査読有り

Miyaguchi Masaki, Nakanishi Yoichi, Maturana Andres D., Mizutani Kimihiko, Niimi Tomoaki

JOURNAL OF MOLECULAR BIOLOGY 434 巻 ( 19 ) 頁： 167777 2022年10月

Precise Quantification of Molybdate In Vitro by the FRET-Based Nanosensor 'MolyProbe' 査読有り国際共著

Oliphant, KD; Karger, M; Nakanishi, Y; Mendel, RR

MOLECULES 27 巻 ( 12 ) 2022年6月

FRET-based detection of isozyme-specific activities of transglutaminases

Tatsukawa, H; Liu, HH; Oba, S; Kamiya, N; Nakanishi, Y; Hitomi, K

AMINO ACIDS 49 巻 ( 3 ) 頁： 615 - 623 2017年3月

Studies on vacuolar membrane microdomains isolated from Arabidopsis suspension-cultured cells: local distribution of vacuolar membrane proteins. 査読有り

Yoshida K, Ohnishi M, Fukao Y, Okazaki Y, Fujiwara M, Song C, Nakanishi Y, Saito K, Shimmen T, Suzaki T, Hayashi F, Fukaki H, Maeshima M, Mimura T.

PLANT AND CELL PHYSIOLOGY 54 巻 ( 10 ) 頁： 1571-1584 2013年7月

Thioredoxin h regulates calcium dependent protein kinases in plasma membranes. 査読有り

Ueoka-Nakanishi H, Sazuka T, Nakanishi Y, Maeshima M, Mori H, Hisabori T.

FEBS J. 280 巻 ( 14 ) 頁： 3220-3231 2013年6月

Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor 査読有り

Nakanishi Y, Iida S, Ueoka-Nakanishi H, Niimi T, Tomioka R, Maeshima M.

PLoS ONE 8 巻 ( 3 ) 頁： e58175 2013年3月

Quantification, organ-specific accumulation and intracellular localization of type II H(+)-pyrophosphatase in Arabidopsis thaliana. 査読有り

PLANT AND CELL PHYSIOLOGY 51 巻 ( 8 ) 頁： 1350-1360 2010年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

Most plants have two types of H(+)-translocating inorganic pyrophosphatases (H(+)-PPases), I and II, which differ in primary sequence and K(+) dependence of enzyme function. Arabidopsis thaliana has three genes for H(+)-PPases: one for type I and two for type II. The type I H(+)-PPase requires K(+) for maximal enzyme activity and functions together with H(+)-ATPase in vacuolar membranes. The physiological role of the type II enzyme, which does not require K(+), is not clear. We focused on the type II enzymes (AtVHP2;1 and AtVHP2;2) of A. thaliana. Total amounts of AtVHP2s were quantified immunochemically using a specific antibody and determined to be 22 and 12 ng mg(-1) of total protein in the microsomal fractions of suspension-cultured cells and young roots, respectively, and the values are approximately 0.1 and 0.2%, respectively, of the vacuolar H(+)-PPase. In plants, AtVHP2s were detected immunochemically in all tissues except mature leaves, and were abundant in roots and flowers. The intracellular localization of AtVHP2s in suspension cells was determined by sucrose density gradient centrifugation and immunoblotting. Comparison with a number of marker proteins revealed localization in the Golgi apparatus and the trans-Golgi network. These results suggest that the type II H(+)-PPase functions as a proton pump in the Golgi and related vesicles in young tissues, although its content is very low compared with the type I enzyme.

Characterization of a tobacco TPK-type K+ channel as a novel tonoplast K+ channel using yeast tonoplasts. 査読有り

Hamamoto S, Marui J, Matsuoka K, Higashi K, Igarashi K, Nakagawa T, Kuroda T, Mori Y, Murata Y, Nakanishi Y, Maeshima M, Yabe I, Uozumi N.

J Biol Chem. 283 巻 ( 4 ) 頁： 1911-1920 2008年1月

Identification of amino acid residues participating in the energy coupling and proton transport of Streptomyces coelicolor A3(2) H+-pyrophosphatase. 査読有り

Hirono M, Nakanishi Y, Maeshima M.

Biochim Biophys Acta. 1767 巻頁： 1401-11 2007年12月

Essential amino acid residues in the central transmembrane domains and loops for energy coupling of Streptomyces coelicolor A3(2) H+-pyrophosphatase. 査読有り

Hirono M, Nakanishi Y, Maeshima M

Biochimica et Biophysica Acta 1767 巻 ( 7 ) 頁： 930-939 2007年7月

プロトン輸送性ピロホスファターゼ：低コスト化合物を利用するポンプ（シンプルなプロトンポンプの機能を支える分子構造）

広野めぐみ，三村久敏，瀬上紹嗣，中西洋一，前島正義

生化学 79 巻 ( 6 ) 頁： 535-541 2007年6月

Expression of functional Streptomyces coelicolor H+-pyrophosphatase and characterization of its molecular properties. 査読有り

Hirono M, Mimura H, Nakanishi Y, Maeshima M

Journal of Biochemistry (Tokyo) 138 巻 ( 2 ) 頁： 183-191 2005年8月

Disulfide-bond formation in the H+-pyrophosphatase of Streptomyces coelicolor and its implications for redox control and enzyme structure. 査読有り

Mimura H, Nakanishi Y, Maeshima M.

FEBS Letter 579 巻 ( 17 ) 頁： 3625-3631 2005年7月

Oligomerization of H(+)-pyrophosphatase and its structural and functional consequences. 査読有り

Mimura H, Nakanishi Y, Maeshima M

Biochimica et Biophysica Acta 1708 巻 ( 3 ) 頁： 393-403 2005年7月

Membrane topology of the H+-pyrophosphatase of Streptomyces coelicolor determined by cysteine-scanning mutagenesis. 査読有り

Mimura H, Nakanishi Y, Hirono M, Maeshima M

The Journal of Biological Chemistry 279 巻 ( 33 ) 頁： 35106-35112 2004年8月

Inverse regulation of rotation of F1-ATPase by the mutation at the regulatory region on the gamma subunit of chloroplast ATP synthase.

Ueoka-Nakanishi, H., Nakanishi, Y., Konno, H., Motohashi, K., Bald, D. and Hisabori, T.

The Journal of Biological Chemistry 279 巻 ( 16 ) 頁： 16272-16277 2004年4月

*Patch clamp analysis of a H+ pump heterologously expressed in giant yeast vacuoles. 査読有り

Nakanishi Y, Yabe I, Maeshima M

Journal of Biochemistry (Tokyo) 134 巻 ( 4 ) 頁： 615-623 2003年10月

Chloroplast cyclophilin is a target protein of thioredoxin. Thiol modulation of the peptidyl-prolyl cis-trans isomerase activity. 査読有り

Motohashi K, Koyama F, Nakanishi Y, Ueoka-Nakanishi H, Hisabori T.

J Biol Chem. 278 巻 ( 34 ) 頁： 31848-31852 2003年8月

Acylspermidine derivatives isolated from a soft coral, Sinularia sp, inhibit plant vacuolar H(+)-pyrophosphatase. 査読有り

Hirono M, Ojika M, Mimura H, Nakanishi Y, Maeshima M

Journal of Biochemistry (Tokyo) 133 巻 ( 6 ) 頁： 811-816 2003年6月

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