Faculty Profiles - TSUKADA, Akira

Papers - TSUKADA, Akira

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Primary structure, tissue distribution, and biological activity of chicken motilin receptor. Reviewed

Ichiro Yamamoto, Hiroyuki Kaiya, Chihiro Tsutsui, Takafumi Sakai, Akira Tsukada, Mikiya Miyazato, Minoru Tanaka

General and Comparative Endocrinology Vol. 156 page： 509-514 2008

Z-chromosome specific primers for chicken-quail hybrid blastoderm. Reviewed

Vol. 44 ( 2 ) page： 209-212 2007.4

Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing a W chromosome. Reviewed

Vol. 86 ( 4 ) page： 731-738 2007

Fertilizing ability of chicken sperm bearing W chromosome. Reviewed

Takagi S., Tsukada A., Saito N. and Shimada K.

Poultry Science Vol. 86 ( 5 ) page： 937-943 2007

Language：English Publishing type：Research paper (scientific journal)

In the sex-reversed domestic fowl, spermatids and sperm carrying the female-specific W chromosome have been demonstrated, but whether the spermatids can become functional sperm and can fertilize the oocyte remains undetermined. In the present study, sex reversal was induced by injection of a nonsteroidal aromatase inhibitor (Fadrozole) into the air sac of the chicken egg on d 4 of incubation, and the chicks were reared to 18 mo old. A single elongated spermatid or sperm was isolated from the testis from either normal roosters or sex-reversed hens, and each was microinjected into a mouse oocyte and cultured for 24 h. Although injected oocytes were monitored on the stage of microscope, they were classified into groups by the number of pronuclei. Those that showed male and female pronuclei (2PN) were considered to have oocyte-activating potency. In the normal rooster group, most semen and testicular sperm induced 2PN, whereas only half of the elongated spermatids induced 2PN. In the sex-reversal group, most testicular sperm induced 2PN, whereas nearly half of the elongated spermatids induced 2PN in the oocytes. There was no pronucleus in the oocytes after microinjection of medium only. A second experiment confirmed the higher rate of oocyte activation by testicular sperm than testicular elongated spermatids. In this second experiment, individual oocytes injected with spermatids and sperm of sex-reversed hens were assayed by PCR to identify the W chromosome. Most spermatids and sperm carried Z chromosome, whereas a minority carried W chromosome. However, the sperm carrying W chromosome evoked 2PN with the same rate of oocyte activation as those carrying Z chromosome. From these results, it is concluded that the chicken elongated spermatids and sperm carrying W chromosome may possess a fertilizing ability similar to normal chicken sperm carrying the Z chromosome.

Primary Structure And Tissue Distribution Of GPR39 mRNA In Japanese Quail,Coturnix japonica. Reviewed

I. Yamamoto, Y. Sakaguchi, M. Numao, A. Tsukada, N. Tsushima and M. Tanaka

Poultly Sience Vol. 2007 ( 86 ) page： 2472-2476 2007

Possible Role of Calcium on Oocyte Development After Intracytoplasmic Sperm Injection in Quail (Coturnix japonica) Reviewed

SHUSEI MIZUSHIMA, SOICHI TAKAGI, TAMAO ONO, YUSUKE ATSUMI, AKIRA TSUKADA, NOBORU SAITO, AND KIYOSHI SHIMADA

JOURNAL OF EXPERIMENTAL ZOOLOGY Vol. 307A page： 1-7 2007

Plasma Prolactin and Corticosterone Concentrations Are Changing Toward hatch with a Different Manner Between Layer- and Broiler- Type Chickens. Reviewed

M. Sato, T. Tachibana, A. Tsukada and M. Furuse.

Journal of Animal and Veterinary Advances Vol. 6 ( 8 ) page： 923-926 2007

Identification of spermatogenic cells expressing protamine mRNA in Japanese quail by RT-PCR.

"Upi Chairun Nisa, Akira Tsukada, Soichi Takagi, Ichiro Yamamoto, Yonju Ha, Anna Hrabia, Noboru Saito and Kiyoshi Shimada."

Journal of Poultry Science Vol. 42(1) page： 70-78. 2005.1

Comparison of mammalian prolactin-releasing peptide and Carassius RFamide for feeding behavior and prolactin secretion in chicks. Reviewed

Tetsuya Tachibana, Akira Tsukada, Masaaki Fujimoto, Hirokazu , Takahashi, Takeshi Ohkubo, Tim Boswell, Mitsuhiro Furuse.

General and Comparative Endocrinology Vol. 144 page： 264-269 2005

Changes in mRNA expression of MMP-2 in the M殕lerian duct of chicken Embryo.

"Ha, Y., Tsukada,A., Sato, N. and Shimada, K."

Gen Comp Endocrinol. Vol. 139(2) page： 131-136. 2004.12

Photoinducible phase specific light induction of Cry1 gene in the pars tuberalis of Japanese quail

"Yasuo S, Watanabe M, Tsukada A, Takagi T, Iigo M, Shimada K, Ebihara S, Yoshimura T"

Endocrinology Vol. 145 page： 1612-1616 2004.4

Effect of central administration of prolactin-releasing peptide on feeding in chicks

"Tetsuya Tachibana, Shin Saito, Shozo Tomonaga, Tomo Takagi, Ei-Suke Saito, Tomonori Nakanishi, Tomoyuki Koutoku, Akira Tsukada, Takeshi Ohkubo, Tim Boswell and Mitsuhiro Furuse"

Physiology & Behavior Vol. 80 page： 713-719 2004.1

cDNA cloning of chicken orexin receptor and tissue distribution: sex dimorphic expression in chicken gonads

"Takeshi Ohkubo, Akira Tsukada and K Shamoto"

Journal of Molecular Endocrinology Vol. 31 page： 499-508 2003.11

cDNA cloning and mRNA Expression of estrogen receptor alpha in the Japanese quail.

"Kouhei Ichikawa, Ichiro Yamamoto, Akira Tsukada, Noboru saito and Kiyoshi Shhimada"

Journal Poultry Science Vol. 40 page： 121-129 2003.4

Effect of Endocrine Disrupters on mRNA Expression of Vitellogenin (VTG) II and Very Low Density Lipoprotein (apoVLDL) II in the Liver of Quail Embryos.

"Kouhei Ichikawa, Yonju Ha, Akira Tsukada, Noboru saito and Kiyoshi Shhimada"

Journal Poultry Science Vol. 40 page： 45-52 2003.2

Profiles of mRNA expression of genes related to sex differentiation of the gonads in the chicken embryo

"Ichiro Yamamoto, Akira Tsukada, Noboru Saito and Kiyoshi Simada"

Poult ry Science Vol. 82 page： 1462-1467 2003.1

Expression of protamine mRNA in relation to spermatogenic activity in Japanese quail

"Upi Chairun Nisa, Akira Tsukada, Masahiko Mori, Noboru Saito and Kiyoshi Shimada"

Journal of Poultry Science Vol. 40 page： 309-318 2003.1

"Effect of Estradiol and Nonylphenol on mRNA Expression of Estrogen Receptors α and β, and Cytochrome P450 Aromatase in the Gonad of Chicken Embryo."

"Masanori Sakimura, Akira Tsukada, Makoto Usami, Shozo Hanzawa, Noboru Saito, Yasuo Ohno and Kiyoshi Shimada."

Journal Poultry Science Vol. 39 page： 302-309 2002.10

cDNA cloning and mRNA expression Transformer 2.(Tra2) in chicken embryo.

"I. Yamamoto, A. Tsukada, N. Saito, K. Simada"

Biochimica et Biophysica Acta Vol. 1579 page： 185-188 2002.9

Suppressive effect of p-nonylphenol on male-specific mRNA expression in the embryonic gonad of chickens.

"Sakimura M, Usami M, Hanzawa S, Tsukada A, Saito N, Yasuo Ohno and Shimada K."

Journal of poultry science Vol. 39 page： 91-99 2002.4

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Papers - TSUKADA, Akira