Faculty Profiles - KASHIDA, Hiromu

Takahashi Hiromi, Yasui Takao, Kashida Hiromu, Makino Koki, Shinjo Keiko, Liu Quanli, Shimada Taisuke, Rahong Sakon, Kaji Noritada, Asanuma Hiroyuki, Baba Yoshinobu

NANOTECHNOLOGY Vol. 32 ( 25 ) 2021.6

Preparation of artificial hexaplex composed of non-natural nucleic acid Reviewed

Koki Makino, Yuhei Hattori, Hiromu Kashida, Hiroyuki Asanuma

CURRENT PROTOCOLS Vol. 1 ( 4 ) 2021.4

A helical amplification system composed of artificial nucleic acids Reviewed

Kashida Hiromu, Nishikawa Keiji, Shi Wenjing, Miyagawa Toshiki, Yamashita Hayato, Abe Masayuki, Asanuma Hiroyuki

CHEMICAL SCIENCE Vol. 12 ( 5 ) 2021.2

Quantitative Analyses of Forster Resonance Energy Transfer between Identical Pyrene Chromophores (Homo-FRET) In DNA Scaffolds. Reviewed

Kashida Hiromu, Kawai Hayato, Azuma Hidenori, Araki Yasuyuki, Wada Takehiko, Asanuma Hiroyuki

CHEMPHOTOCHEM Vol. 5 ( 2 ) page： 167 - 172 2021.2

Intrastrand backbone-nucleobase interactions stabilize unwound right-handed helical structures of heteroduplexes of L-<i>a</i>TNA/RNA and SNA/RNA Reviewed

Kamiya, Y; Satoh, T; Kodama, A; Suzuki, T; Murayama, K; Kashida, H; Uchiyama, S; Kato, K; Asanuma, H

COMMUNICATIONS CHEMISTRY Vol. 3 ( 1 ) 2020.11

Efficient Light-Harvesting Antennae Resulting from the Dense Organization of Dyes into DNA Junctions though d-Threoninol Reviewed

Kashida Hiromu, Azuma Hidenori, Maruyama Ryoko, Araki Yasuyuki, Wada Takehiko, Asanuma Hiroyuki

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION Vol. 59 ( 28 ) page： 11360 - 11363 2020.7

A triplex-forming linear probe for sequence-specific detection of duplex DNA with high sensitivity and affinity Reviewed

Chen Yanglingzhi, Murayama Keiji, Kashida Hiromu, Kamiya Yukiko, Asanuma Hiroyuki

CHEMICAL COMMUNICATIONS Vol. 56 ( 40 ) page： 5358 - 5361 2020.5

Nanometer Accuracy in Cryogenic Far-Field Localization Microscopy of Individual Molecules Reviewed

Furubayashi Taku, Ishida Keita, Kashida Hiromu, Nakata Eiji, Morii Takashi, Matsushita Michio, Fujiyoshi Satoru

JOURNAL OF PHYSICAL CHEMISTRY LETTERS Vol. 10 ( 19 ) page： 5841 - 5846 2019.10

Selective binding of nucleosides to gapped DNA duplex revealed by orientation and distance dependence of FRET Reviewed

Kashida Hiromu, Kokubo Yuta, Makino Koki, Asanuma Hiroyuki

ORGANIC & BIOMOLECULAR CHEMISTRY Vol. 17 ( 28 ) page： 6786 - 6789 2019.7

N-Benzoyl-protected Peptide Nucleic Acid (PNA) Monomers Expand the Range of Nucleobases Available for PNA-DNA Chimera Reviewed

Inagaki Masahito, Uematsu Ryohei, Mizutani Tatsuya, Unabara Daisuke, Araki Yasuyuki, Sakamoto Seiji, Kashida Hiromu, Nishijima Masaki, Asanuma Hiroyuki, Inoue Yoshihisa, Wada Takehiko

CHEMISTRY LETTERS Vol. 48 ( 4 ) page： 341 - 344 2019.4

In-stem molecular beacon targeted to a 5 '-region of tRNA inclusive of the D arm that detects mature tRNA with high sensitivity Reviewed

Miyoshi Yuichi, Ohtsuki Takashi, Kashida Hiromu, Asanuma Hiroyuki, Watanabe Kazunori

PLOS ONE Vol. 14 ( 1 ) 2019.1

Photo-regulatable DNA isothermal amplification by template-mediated ligation Reviewed

Cheng Bohao, Kashida Hiromu, Shimada Naohiko, Maruyama Atsushi, Asanuma Hiroyuki

CHEMICAL COMMUNICATIONS Vol. 55 ( 8 ) page： 1080 - 1083 2019.1

The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons

Murayama Keiji, Kashida Hiromu, Asanuma Hiroyuki

NON-NATURAL NUCLEIC ACIDS: METHODS AND PROTOCOLS Vol. 1973 page： 261 - 279 2019

Quantitative evaluation of energy migration between identical chromophores enabled by breaking symmetry Reviewed

Kashida Hiromu, Kawai Hayato, Maruyama Ryoko, Kokubo Yuta, Araki Yasuyuki, Wada Takehiko, Asanuma Hiroyuki

COMMUNICATIONS CHEMISTRY Vol. 1 2018.12

The DNA Duplex as an Aqueous One-Dimensional Soft Crystal Scaffold for Photochemistry Invited Reviewed

Asanuma Hiroyuki, Murayama Keiji, Kamiya Yukiko, Kashida Hiromu

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN Vol. 91 ( 12 ) page： 1739 - 1748 2018.12

Bifacial nucleobases for hexaplex formation in aqueous solution Reviewed

H. Kashida, Y. Hattori, K. Tazoe, T. Inoue, K. Nishikawa, K. Ishii, S. Uchiyama, H. Yamashita, M. Abe, Y. Kamiya, H. Asanuma

J. Am. Chem. Soc. Vol. 140 ( 27 ) page： 8456 - 8462 2018.7

Chaperone polymer-assisted, photo-driven DNA strand displacement Reviewed

B. Cheng, H. Kashida, N. Shimada, A. Maruyama, H. Asanuma

ChemBioChem Vol. 18 ( 16 ) page： 1568 - 1572 2017.8

Orientation-dependent FRET system reveals differences in structures and flexibilities of nicked and gapped DNA duplexes Reviewed

H. Kashida, A. Kurihara, H. Kawai, H. Asanuma

Nucleic Acids Res. Vol. 45 ( 11 ) page： e105 2017.6

Development of Pseudo Base-Pairs on d-Threoninol which Exhibit Various Functions Invited Reviewed

H. Kashida, H. Asanuma

Bull. Chem. Soc. Jpn. Vol. 90 ( 5 ) page： 475 - 484 2017.5

Design of photofunctional oligonucleotides by copolymerization of natural nucleobases with base surrogates prepared from acyclic scaffolds Invited Reviewed

H. Asanuma, K. Murayama, Y. Kamiya, H. Kashida

Polymer J. Vol. 49 ( 3 ) page： 279 - 289 2017.3

DNA二重鎖による色素会合体の精密位置配向制御と光化学への展開 Invited

浅沼浩之、樫田啓

固体物理 Vol. 52 ( 3 ) page： 149-158 2017

Development of Pseudo Base Pairs Which Show High DNA Duplex Stabilities and Orthogonality Invited Reviewed

Kashida, H; Asanuma, H

KOBUNSHI RONBUNSHU Vol. 74 ( 4 ) page： 257 - 264 2017

Strand-invading linear probe combined with unmodified PNA Reviewed

H. Asanuma, R. Niwa, M. Akahane, K. Murayama, H. Kashida, Y. Kamiya

Bioorg. Med. Chem. Vol. 24 page： 4129-4137 2016.9

Faint electric treatment-induced rapid endosomal escape for efficient delivery of functional macromolecules into the cytoplasm Reviewed

M. Hasan, A. Nishimoto, T. Ohgita, S. Hama, H. Kashida, H. Asanuma, K. Kogure

J. Control Release Vol. 228 page： 20-25 2016

Acyclic artificial nucleic acids with phosphodiester bonds exhibit unique functions" Reviewed

H. Kashida, K. Murayama, H. Asanuma

Polymer J. Vol. 48 page： 781-786 2016

Visible Light-Triggered Crosslinking of DNA Duplex by Reversible [2+2] Photocycloaddition of Styrylpyrene Reviewed

T. Doi, H. Kawai, K. Murayama, H. Kashida, H. Asanuma

Chem. Eur. J. Vol. 22 page： 10533-10538 2016

A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection Reviewed

H. Kashida, K. Morimoto, H. Asanuma

Sci. Technol. Adv. Mater. Vol. 17 page： 267-273 2016

新規人工核酸SNAを用いたRNAイメージング Invited

樫田啓、村山恵司、浅沼浩之

生体の科学 Vol. 66 ( 2 ) page： 145-150 2015

Ultrasensitive Molecular Beacon Designed with Totally Serinol Nucleic Acid (SNA) for Monitoring mRNA in Cells Reviewed

K. Murayama, Y. Kamiya, H. Kashida, H. Asanuma

ChemBioChem Vol. 16 ( 9 ) page： 1298-1301 2015

Efficiency of [2+2] photodimerization of various stilbene derivatives within the DNA duplex scaffold Reviewed

T. Doi, H. Kashida, H. Asanuma

Org. Biomol. Chem. Vol. 13 ( 15 ) page： 4430-4437 2015

Acyclic L-threoninol nucleic acid (L-aTNA) with suitable structural rigidity cross-pairs with DNA and RNA Reviewed

K. Murayama, H. Kashida, H. Asanuma

Chem. Commun. Vol. 51 ( 30 ) page： 6500-6503 2015

Molecular design of Cy3 derivative for highly sensitive in-stem molecular beacon and its application to the wash-free FISH Reviewed

H. Kashida, T. Osawa, K. Morimoto, Y. Kamiya, H. Asanuma

Bioorg. Med. Chem. Vol. 23 ( 8 ) page： 1758-1762 2015

Highly Sensitive and Robust Linear Probe for Detection of mRNA in Cells Reviewed

H. Asanuma, M. Akahane, R. Niwa, H. Kashida, Y. Kamiya

Angew. Chem. Int. Ed. Vol. 54 ( 14 ) page： 4315-4319 2015

Hetero-Selective DNA-like Duplex Stabilized by Donor-Acceptor Interaction Reviewed

T. Doi, T. Sakibara, H. Kashida, Y. Araki, T. Wada, H. Asanuma

Chem. Eur. J. Vol. 21 page： 15974-15980 2015

Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2 Reviewed

Y. Kamiya, K. Iishiba, T. Doi, K. Tsuda, H. Kashida, H. Asanuma

Biomater. Sci. Vol. 3 page： 1534-1538 2015

DNA二重鎖を足場とした配向依存的FRET Invited

浅沼浩之、栗原綾子、樫田啓

光化学 Vol. 45 page： 146-147 2014

De Novo Design of Functional Oligonucleotides with Acyclic Scaffolds Reviewed

H. Asanuma, H. Kashida, Y. Kamiya

Chem. Rec. Vol. 14 ( 6 ) page： 1055-1069 2014

Enhancement of Stability and Activity of siRNA by Terminal Substitution with Serinol Nucleic Acid (SNA) Reviewed

Y. Kamiya, J. Takai, H. Ito, K. Murayama, H. Kashida, H. Asanuma

ChemBioChem Vol. 15 ( 17 ) page： 2549-2555 2014

Selective labeling of mature RISC using siRNA carrying fluorophore-quencher pair Invited Reviewed

Y. Kamiya, A. Ito, H. Ito, M. Urushihara, J. Takai, T. Fujii, X.G. Liang, H. Kashida, H. Asanuma

Chem. Sci. Vol. 4 page： 4016-4021 2013

The "sugar-deficient" G-quadruplex: incorporation of aTNA in quadruplex structures Invited Reviewed

J. Zhou, K. Murayama, S. Amrane, H. Kashida, A. Bourdoncle, H. Asanuma, J. L. Mergny

Chem. Sci. Vol. 4 page： 3693-3698 2013

Highly stable duplex formation by artificial nucleic acids aTNA and SNA with acyclic scaffolds Invited Reviewed

K. Murayama, Y. Tanaka, T. Toda, H. Kashida, H. Asanuma

Chem. Eur. J. Vol. 19 page： 14151-14158 2013

p-Stilbazole moieties as artificial base pairs for photocrosslinking of DNA duplex Reviewed

H. Kashida, T. Doi, T. Sakakibara, T. Hayashi, H. Asanuma

J. Am. Chem. Soc. Vol. 135 page： 7960-7966 2013

Evaluation of intrinsic spectroscopic properties of chromophore assemblies by shielding with cyclohexyl base pairs within a DNA duplex Invited Reviewed

H. Kashida, N. Higashiyama, T. Kato, H. Asanuma

Bioorg. Med. Chem. Vol. 21 ( 20 ) page： 6191-6197 2013

Development of a robust model system of FRET using base-surrogates tethering fluorophores for strict control of their position and orientation within DNA duplex Reviewed

T. Kato, H. Kashida, H. Kishida, H. Yada, H. Okamoto, H. Asanuma

J. Am. Chem. Soc. Vol. 135 page： 741-750 2013

Highly Stable Duplex Formation by Artificial Nucleic Acids Acyclic Threoninol Nucleic Acid (aTNA) and Serinol Nucleic Acid (SNA) with Acyclic Scaffolds Reviewed

K. Murayama, Y. Tanaka, T. Toda, H. Kashida, H. Asanuma

Chem. Eur. J. Vol. 19 ( 42 ) page： 14151-14158 2013

SNA：配列設計でらせん構造が制御可能な核酸アナログ

浅沼浩之、村山恵司、樫田啓

高分子 Vol. 62 page： 513-514 2013

Design of an Artificial Functional Nanomaterial with High Recognition Ability Reviewed

X.G. Liang, T. Mochizuki, T. Fujii, H. Kashida, H. Asanuma

Natural Computing Vol. 11 page： 231-238 2012

Reversed assembling of dyes in RNA duplex compared with those in DNA Reviewed

T. Fujii, M. Urushihara, H. Kashida, H. Ito, X.G. Liang, M. Yagi-Utsumi, K. Kato, H. Asanuma

Chem. Eur. J. Vol. 18 page： 13304-13313 2012

Bulge-like asymmetric hetero dye-clustering in DNA duplex results in efficient quenching of background emission based on the maximized excitonic interaction Reviewed

T. Fujii, Y. Hara, T. Osawa, H. Kashida, X.G. Liang, Y. Yoshida, H. Asanuma

Chem. Eur. J. Vol. 18 page： 10865-10872 2012

Quencher-free molecular beacon tethering 7-hydroxycoumarin detects targets through protonation/deprotonation Reviewed

H. Kashida, K. Yamaguchi, Y. Hara, H. Asanuma

Bioorg. Med. Chem. Vol. 20 page： 4310-4315 2012

Preparation of supramolecular chromophoric assemblies using a DNA duplex Reviewed

H. Kashida, H. Asanuma

Phys. Chem. Chem. Phys. Vol. 14 page： 7196-7204 2012

Quencher-free linear probe with multiple fluorophores on acyclic scaffold Reviewed

H. Asanuma, M. Akahane, N. Kondo, T. Osawa, T. Kato, H. Kashida

Chem. Sci. page： accepted. 2012

Coherent Interactions of Dyes Assembled on DNA Invited Reviewed

H. Asanuma, T. Fujii, T. Kato, H. Kashida

J. Photochem. Photobiol. C Vol. 13 page： 1065-1084 2012

Polycation-chaperoned in-stem molecular beacon system Reviewed

H. Asanuma, T. Osawa, H. Kashida, T. Fujii, X.G. Liang, K. Niwa, Y. Yoshida, N. Shimada, A. Maruyama

Chem. Commun. Vol. 48 page： 1760-1762 2012

Design of a Functional Nanomaterial with Recognition Ability for Constructing Light-Driven Nanodevices Reviewed

X. G. Liang, T. Mochizuki, T. Fujii, H. Kashida, H. Asanuma

LCNS Vol. 6518 page： 112-122 2011

Detection of three-base deletion by exciplex formation with perylene derivatives Reviewed

H. Kashida, N. Kondo, K. Sekiguchi, H. Asanuma

Chem. Commun. Vol. 47 page： 6404-6406 2011

Cationic dye-triplet as unique glue that can connect full-matched termini of the duplexes Reviewed

H. Kashida, T. Hayashi, T. Fujii, H. Asanuma

Chem. Eur. J. Vol. 17 page： 2614-2622 2011

Control of the Chirality and Helicity of Oligomers of Serinol Nucleic Acid (SNA) by Sequence Design." Reviewed

H. Kashida, K. Murayama, T. Toda, H. Asanuma

Angew. Chem. Int. Ed. Vol. 50 page： 1285-1288 2011

Cyclohexyl base pairs stabilize duplexes and intensify pyrene fluorescence by shielding it from natural base pairs Reviewed

H. Kashida, K. Sekiguchi, N. Higashiyama, T. Kato, H. Asanuma

Org. Biomol. Chem. Vol. 9 page： 8313-8320 2011

Insulator base pairs for lighting-up perylenediimide in a DNA duplex. Reviewed

H. Kashida, K. Sekiguchi, H. Asanuma.

Chem. Eur. J. Vol. 16 page： 11554-11557 2010

Coherent Quenching of a Fluorophore for the Design of a Highly Sensitive In-Stem Molecular Beacon Reviewed

Y. Hara, T. Fujii, H. Kashida, K. Sekiguchi, X. G. Liang, K. Niwa, T. Takase, Y. Yoshida, H. Asanuma.

Angew. Chem. Int. Ed. Vol. 49 page： 5502-5506 2010

Accumulation of fluorophores into DNA duplexes to mimic the properties of quantum dots Reviewed

J. Am. Chem. Soc. Vol. 132 page： 6223-6230 2010

An efficient FRET between pyrene and perylene assembled in a DNA duplex and its potential for discriminating single base changes. Reviewed

H. Kashida, T. Takatsu, K. Sekiguchi, H. Asanuma

Chem. Eur. J. Vol. 16 page： 2479-2486 2010

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

Fluorescence resonance energy transfer between the donor (pyrene) and acceptor (perylene) was observed within the DNA double helix. In our design, chromophores were arranged in a pre-determined order and position. Furthermore, a one-base deletion was successfully detected with a large apparent Stokes' shift.

Unexpectedly stable artificial duplex from flexible acyclic threoninol Reviewed

H. Asanuma, T. Toda, K. Murayama, X. G. Liang, H. Kashida

J. Am. Chem. Soc. Vol. 132 page： 14702-14703 2010

Rational design of functional DNA with a non-ribose acyclic scaffold Reviewed

H. Kashida, X. G. Liang, H. Asanuma

Curr. Org. Chem. Vol. 13 page： 1065-1084 2009.7

Modulation of pKa of Brooker's Merocyanine by DNA Hybridization Reviewed

H. Kashida, K. Sano, Y. Hara, H. Asanuma.

Bioconjugate Chem. Vol. 20 page： 258-265 2009.2

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

Brooker's merocyanine (BM), which changes its emission and absorption maxima upon protonation, was introduced into ODN via D-threoninol. The pK(a) of BM in the modified ODN increased from 9.5 to 10.1 upon hybridization. Ratiometric detection of hybridization was also possible by simultaneous excitation of both protonated and deprotonated BMs. Furthermore, we could also modulate its pK(a) by the antiparallel stacking of two BM molecules in the duplex. Thus, control of the microenvironment around the BM molecule allowed modulation of its pK(a), which is applicable to the sequence-specific recognition of target DNA.

*Positively charged base surrogate for highly stable “base-pairing" through electrostatic and stacking interactions Reviewed

H. Kashida, H. Ito, T. Fujii, T. Hayashi, H. Asanuma

J. Am. Chem. Soc. Vol. 131 page： 9928-9930 2009

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

"Base pairs" of cationic dyes (p-methylstilbazole) were incorporated into oligodeoxyribonucleotides (ODNs). This "base pair" greatly stabilized the duplex through electrostatic and stacking interactions. The melting temperature of modified ODN was higher than those of neutral dyes and native base pairs. Further stabilization of the duplex was observed when the number of cationic dyes increased.

第３の塩基対をつくる

樫田啓

化学 Vol. 11 page： 70-71 2009

Efficient energy transfer from pyrene to perylene assembled inside DNA duplex

H. Kashida, T. Takatsu, H. Asanuma

Nucleic Acids Symp. Ser. Vol. 53 page： 29-30 2009

*In-Stem Molecular Beacon containing a Pseudo Base Pair of Threoninol Nucleotides for Removal of Background Emission. Reviewed

H. Kashida, T. Takatsu, T. Fujii, K. Sekiguchi, X. G. Liang, K. Niwa, T. Takase, Y. Yoshida, H. Asanuma.

Angew. Chem. Int. Ed. Vol. 48 page： 7044-7047 2009

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

An in-stem molecular beacon (IS-MB), which tethers perylene and anthraquinone in the stem region via D-threoninol, effectively detected target sequences. This design of IS-MB is also applicable to the discrimination of a one-base deletion mutant from wild type (full-match) without background emission.

Analysis of Coherent Heteroclustering of Different Dyes by Use of Threoninol-Nucleotides for Comparison with the Molecular Exciton Theory Reviewed

T. Fujii, H. Kashida, H. Asanuma

Chem. Eur. J. Vol. 15 page： 10092-10102 2009

Postsynthetic modification of DNA via threoninol on a solid support by means of allylic protection. Reviewed

H. Asanuma, Y. Hara, A. Noguchi, K. Sano, H. Kashida

Tetrahedron Lett. Vol. 49 page： 5144-5146 2008.7

Threoninol as a Scaffold of Dyes (Threoninol-nucleotide) and Their Stable Interstrand Clustering in Duplexes. Reviewed

H. Kashida, T. Fujii, H. Asanuma

Org. Biomol. Chem. Vol. 6 page： 2892-2899 2008.6

Language：English Publishing type：Research paper (scientific journal)

Functional molecules such as dyes (Methyl Red, azobenzene, and Naphthyl Red) were tethered on D-threoninol as base surrogates (threoninol-nucleotide), which were consecutively incorporated at the center of natural oligodeoxyribonucleoticles (ODNs). Hybridization of two ODNs involving threoninol-nucleotides allowed interstrand clustering of the dyes on D-threoninol and greatly stabilized the duplex. Clustering of Methyl Reds induced both hypsochromicity and narrowing of the band, demonstrating that Methyl Reds were axially stacked relative to each other (H-aggregation). Alternate hetero dye clusters could also be prepared only by hybridization of two ODNs with different threoninol-nucleotides, such as Methyl Red-azobenzene and Methyl Red-Naphthyl Red combinations. Thus, D-threoninol can be a versatile scaffold for introducing functional molecules into DNA for their ordered clustering.

Preparation of coherent hetero clusters with threoninol scaffold

T. Fujii, H. Kashida, H. Asanuma

Nucleic Acids Symp. Ser. Vol. 52 page： 699-700 2008

Incorporation of cationic dyes into DNA for distinct stabilization of duplex

H. Kashida, H. Itoh, T. Fujii, H. Asanuma.

Nucleic Acids Symp. Ser. Vol. 52 page： 701-702 2008

Detection of genetic polymorphisms with high sensitivityby DNA–perylene conjugate Reviewed

H. Kashida, T. Takatsu, H. Asanuma

Tetrahedron Lett. Vol. 48 page： 6759-6762 2007.7

2',6'-Dimethylazobenzene as an efficient and thermo-stable photo-regulator for the photoregulation of DNA hybridization. Reviewed

H. Nishioka, X. G. Liang, H. Kashida, H. Asanuma

Chem. Commun. page： 4354-4356 2007

Development of high-sensitive DNA probe by using perylene.

H. Kashida, T. Takatsu, H. Asanuma

Nucleic Acids Symp. Ser. Vol. 51 page： 279-280 2007

Preparation of "comb-type" hetero aggregates by DNA-dye conjugation.

T. Fujii, H. Kashida, H. Asanuma

Nucleic Acids Symp. Ser. Vol. 51 page： 277-278 2007

Construction of novel dye aggregates based on "comb-type" sequence.

H. Kashida, T. Fujii, H. Asanuma

Nucleic Acids Symp. Ser. Vol. 51 page： 11-12 2007

Exciplex formation between pyrene and N,N-dimethylaniline in DNA for the detection of one-base deletion Reviewed

H. Kashida, M. Komiyama, H. Asanuma

Chem. Lett. Vol. 35 ( 8 ) page： 934-035 2006.8

Covalent incorporation of methyl red dyes into double-stranded DNA for their ordered clustering Reviewed

H. Kashida, M. Tanaka, S. Baba, T. Sakamoto, G. Kawai, H. Asanuma, M. Komiyama

Chem. --Eur. J. Vol. 12 ( 3 ) page： 777-784 2006.1

Insertion of two pyrene moieties into oligodeoxyribonucleotides for the efficient detection of deletion polymorphisms Reviewed

H. Kashida, H. Asanuma, M. Komiyama

Chem. Commun. ( 26 ) page： 2768-2770 2006.1

Language：English Publishing type：Research paper (scientific journal)

For the detection of deletion polymorphisms, two pyrene moieties are tethered to oligodeoxyribonucleotide (ODN) on both sides of the intervening base. When this probe ODN was hybridized with wild type ODN, both pyrenes intercalated between base pairs and only monomer emission was observed. In contrast, strong excimer emission was generated by hybridization with one-base deletion mutant. Similarly, two-base deletion was also detected.

Enhancement of RNA cleavage activity of 10-23 DNAzyme by covalently introduced intercalator Reviewed

H. Asanuma, H. Hayashi, J. Zhao, X. G. Liang, A. Yamazawa, T. Kuramochi, D. Matsunaga, Y. Aiba, H. Kashida, M. Komiyama

Chem. Commun. ( 48 ) page： 5062-5064 2006

Incorporation of methyl group on azobenzene for the effective photo-regulation of hybridization and suppression of thermal isomerization

H. Nishioka, H. Kashida, M. Komiyama, X. G. Liang, H. Asanuma

Nucleic Acids Symp. Ser. Vol. 50 page： 85-86 2006

Interstrand H-aggregation of cationic dyes for narrowing the absorption spectra and stabilizing the duplex Reviewed

H. Kashida, H. Asanuma, M. Komiyama

Supramol. Chem. Vol. 16 ( 6 ) page： 459-464 2004.9

Alternating hetero H aggregation of different dyes by interstrand stacking from two DNA-dye conjugates

H. Kashida, H. Asanuma, M. Komiyama

Angew. Chem. Int. Ed. Vol. 43 ( 47 ) page： 6522-6525 2004

Language：English Publishing type：Research paper (scientific journal)

Heteroaggregates in which two dyes are stacked alternately can be prepared by interstrand stacking from two DNA–dye conjugates. Even different dyes can exhibit an H band as a result of aggregation. The present method is applicable to other functional molecules, and various novel materials can be produced by the attachment of dyes to DNA.

光機能性DNAの設計と応用

浅沼浩之、樫田啓

高分子加工 Vol. 53 ( 1 ) page： 37-43 2004

NMR study on the photoresponsive DNA tethering an azobenzene. Assignment of the absolute configuration of two diastereomers and structure determination of their duplexes in the trans-form Reviewed

X. G. Liang, H. Asanuma, H. Kashida, A. Takasu, T. Sakamoto, G. Kawai, M. Komiyama

J. Am. Chem. Soc. Vol. 125 ( 52 ) page： 16408-16415 2003.12

DNA-dye conjugates for controllable H* aggregation Reviewed

H. Asanuma, K. Shirasuka, T. Takarada, H. Kashida, M. Komiyama

J. Am. Chem. Soc. Vol. 125 ( 8 ) page： 2217-2223 2003.12

DNA-Naphthyl Red conjugate as a visualizing probe of DNA hybridization Reviewed

H. Asanuma, H. Kashida, X. G. Liang, M. Komiyama

Chem. Commun. ( 13 ) page： 1536-1537 2003

Development of a probe DNA which accompanies color change on hybridization

H. Kashida, H. Asanuma, M. Komiyama

Nucleic Acids Res. Suppl. Vol. 3 page： 143-144 2003

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Papers - KASHIDA, Hiromu