Faculty Profiles - KOJIMA, Seiji

Papers - KOJIMA, Seiji

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Chemotactic responses to an attractant and a repellent by the polar and lateral flagellar systems of Vibrio alginolyticus Reviewed

M Homma, H Oota, S Kojima, Kawagishi, I, Y Imae

MICROBIOLOGY-UK Vol. 142 page： 2777 - 2783 1996.10

Language：English Publishing type：Research paper (scientific journal) Publisher：SOC GENERAL MICROBIOLOGY

Chemotactic responses in Vibrio alginolyticus, which has lateral and polar flagellar systems in one cell, were investigated. A lateral-flagella-defective (Pof(+) Laf(-)) mutant, which has only a polar flagellum, usually swam forward by the pushing action of its flagellum and occasionally changed direction by backward swimming. When the repellent phenol was added, Pof(+) Laf(-) cells moved frequently forward and backward (tumbling state). The tumbling was derived from the frequent changing between counter-clockwise and clockwise (CW) rotation of the flagellar motor, as was confirmed by the tethered-cell method. Furthermore, we found that the tumbling cells did not adapt to the phenol stimulus. When the attractant serine was added, the phenol-treated cells ceased tumbling and swam smoothly, adapting to the attractant stimulus after several minutes. We isolated chemotaxis-defective (Che(-)) mutants from the Pof(+) Laf(-) mutant; the tumbling mutants were not isolated. One interesting mutant swam backwards continuously, with its flagellum leading the cell and its flagellar motor rotating CW continuously. A polar-flagella-defective mutant (Pof(-) Laf(+)) stopped swimming after phenol addition and then recovered swimming ability within 10 min, indicating that lateral flagella can adapt to the repellent stimulus. This may represent a functional difference between the two flagellar systems in Vibrio cells, and between the chemotaxis systems affecting the two types of flagella.

DOI： 10.1099/13500872-142-10-2777

Web of Science

Chemotactic responses to an attractant and a repellent by the polar and lateral flagellar systems of Vibrio alginolyticus

Homma M, Oota H, Kojima S, Kawagishi I, Imae Y

MICROBIOLOGY-UK Vol. 142 page： 2777 - 2783 1996.10

Chemotactic responses to an attractant and a repellent by the polar and lateral flagellar systems of Vibrio alginolyticus.

Homma, M., Oota, H., Kojima, S., Kawagishi, I. & Imae, Y.

Microbiology Vol. 142 page： 2777-2783 1996

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Papers - KOJIMA, Seiji