Updated on 2024/03/28

写真a

 
OHKAWA-NISHIWAKI, Taeko
 
Organization
Graduate School of Bioagricultural Sciences Department of Animal Sciences Associate professor
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences Department of Bioresource Sciences
Title
Associate professor
Contact information
メールアドレス

Degree 1

  1. 博士(理学) ( 1998.3   総合研究大学院大学 ) 

Research Areas 3

  1. Life Science / Functional biochemistry

  2. Others / Others  / Chronobiology

  3. Life Science / Animal physiological chemistry, physiology and behavioral biology

Education 1

  1. The Graduate University for Advanced Studies   Graduate School, Division of Life Science

    - 1998.3

      More details

    Country: Japan

Professional Memberships 4

  1. 日本時間生物学会   評議員 編集委員

  2. 日本生化学会

  3. 日本生物物理学会

  4. 東海畜産学会   評議員

    2017.4

 

Papers 36

  1. Level of constitutively expressed BMAL1 affects the robustness of circadian oscillations Reviewed

    Padlom, A; Ono, D; Hamashima, R; Furukawa, Y; Yoshimura, T; Nishiwaki-Ohkawa, T

    SCIENTIFIC REPORTS   Vol. 12 ( 1 ) page: 19519   2022.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-022-24188-4

    Web of Science

    PubMed

  2. Regulation mechanisms of the dual ATPase in KaiC Reviewed

    Furuike, Y; Mukaiyama, A; Koda, SI; Simon, D; Ouyang, DY; Ito-Miwa, K; Saito, S; Yamashita, E; Nishiwaki-Ohkawa, T; Terauchi, K; Kondo, T; Akiyama, S

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 119 ( 19 ) page: e2119627119   2022.5

     More details

  3. Modulation of circadian clock by crude drug extracts used in Japanese Kampo medicine

    Zhang, MH; Kobayashi, K; Atsumi, H; Katada, Y; Nakane, Y; Chen, JF; Nagano, R; Kadofusa, N; Nishiwaki-Ohkawa, T; Kon, N; Hirota, T; Sato, A; Makino, T; Yoshimura, T

    SCIENTIFIC REPORTS   Vol. 11 ( 1 ) page: 21038   2021.10

     More details

  4. Photoperiodic regulation of dopamine signaling regulates seasonal changes in retinal photosensitivity in mice

    Okimura, K; Nakane, Y; Nishiwaki-Ohkawa, T; Yoshimura, T

    SCIENTIFIC REPORTS   Vol. 11 ( 1 ) page: 1843   2021.1

     More details

  5. Seasonal changes in NRF2 antioxidant pathway regulates winter depression-like behavior

    Nakayama, T; Okimura, K; Shen, J; Guh, YJ; Tamai, TK; Shimada, A; Minou, S; Okushi, Y; Shimmura, T; Furukawa, Y; Kadofusa, N; Sato, A; Nishimura, T; Tanaka, M; Nakayama, K; Shiina, N; Yamamoto, N; Loudon, AS; Nishiwaki-Ohkawa, T; Shinomiya, A; Nabeshima, T; Nakane, Y; Yoshimura, T

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 117 ( 17 ) page: 9594 - 9603   2020.4

     More details

  6. Casein kinase 1 family regulates PRR5 and TOC1 in the Arabidopsis circadian clock

    Uehara, TN; Mizutani, Y; Kuwata, K; Hirota, T; Sato, A; Mizoi, J; Takao, S; Matsuo, H; Suzuki, T; Ito, S; Saito, AN; Nishiwaki-Ohkawa, T; Yamaguchi-Shinozaki, K; Yoshimura, T; Kay, SA; Itami, K; Kinoshita, T; Yamaguchi, J; Nakamichi, N

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 116 ( 23 ) page: 11528 - 11536   2019.6

     More details

  7. Identification of circadian clock modulators from existing drugs

    Tamai T. Katherine, Nakane Yusuke, Ota Wataru, Kobayashi Akane, Ishiguro Masateru, Kadofusa Naoya, Ikegami Keisuke, Yagita Kazuhiro, Shigeyoshi Yasufumi, Sudo Masaki, Nishiwaki-Ohkawa Taeko, Sato Ayato, Yoshimura Takashi

    EMBO MOLECULAR MEDICINE   Vol. 10 ( 5 )   2018.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.15252/emmm.201708724

    Web of Science

    PubMed

  8. Combination of synthetic chemistry and live-cell imaging identified a rapid cell division inhibitor in Tobacco and Arabidopsis thaliana Reviewed

    Nambo M, Kurihara D, Yamada T, Nishiwaki-Ohkawa T, Kadofusa N, Kimata Y, Kuwata K, Umeda M, Ueda M

    Plant and Cell Physiology   Vol. 57 ( 11 ) page: 2255-2268   2016.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcw140

  9. Molecular basis for regulating seasonal reproduction in vertebrates Invited Reviewed

    Nishiwaki-Ohkawa T, Yoshimura T

    Journal of Endocrinology   Vol. 229   page: R117-R127   2016.4

     More details

    Authorship:Lead author   Language:English  

    DOI: 10.1530/JOE-16-0066

  10. Electrochemical Detection of Circadian redox rhythm in cyanobacterial cells via extracellular electron transfer. Reviewed

    1. Nishio, K., Pornpitra, T., Izawa, S., Nishiwaki-Ohkawa, T., Kato, S., Hashimoto, K., and Nakanishi, S.

    Plant Cell Physiology     2015.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcv066

  11. C-H activation generates period-shortening molecules that target Cryptochrome in the mammalian circadian clock. Reviewed

    Oshima, T., Yamanaka, I., Kumar, A., Yamaguchi, J., Nishiwaki-Ohkawa, T., Muto, K., Kawamura, R., Hirota T., Yagita, K., Irle, S., Kay, S.A., Yoshimura, T., and Itami, K.

    Angewandte Chemie International Edition     2015.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/anie.201502942

  12. Tissue-specific post-translational modification allows functional targeting of thyrotropin. Reviewed

    Ikegami, K., Liao, X.-H., Hoshino, Y., Ono, H., Ota, W., Ito, Y., Nishiwaki-Ohkawa, T., Sato, C., Kitajima, K., Iigo, M., Shigeyoshi Y., Yamada, M., Murata, Y., Refetoff, S and Yoshimura T.

    Cell Reports   Vol. 9 ( 3 ) page: 801-809   2014.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.celrep.2014.10.006

  13. Exchange of ADP with ATP in the CII ATPase domain promotes autophosphorylation of cyanobacterial clock protein KaiC Reviewed

    Taeko Nishiwaki-Ohkawa, Yohko Kitayama, Erika Ochiai, and Takao Kondo

    Proceedings of National Academy of Sciences of USA   Vol. 111   page: 4455-4460   2014.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  14. Regulation of seasonal reproduction by hypothalamic activation of thyroid hormone. Reviewed

    Shinomiya A., Shimmura T., Nishiwaki-Ohkawa, T., Yoshimura T

    Frontiers in Endocrinology     2014.2

     More details

    Language:English  

    DOI: 10.3389/fendo.2014.00012

  15. Intersubunit communications within KaiC hexamers contribute the robust rhythmicity of the cyanobacterial circadian clock. Reviewed

    Kitayama Y., Nishiwaki-Ohkawa T., Kondo T.

    Microbial Cell   Vol. 1   page: 67-69   2014.1

     More details

    Language:English  

  16. KaiC intersubunit communication facilitates robustness of circadian rhythm in cyanobacteria Reviewed

    Kitayama Y., Nishiwaki-Ohkawa, T., Sugisawa Y. Kondo T

    Nature Communications     2013.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/ncomms 3897

  17. Circadian autodephosphorylation of cyanobacterial clock protein KaiC occurs via formation of ATP as intermediate Reviewed

    Nishiwaki, T., Kondo, T.

    The Journal of Biological Chemistry   Vol. 287   page: 18030-18035   2012

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  18. Dual KaiC-based oscillations constitute the circadian system of cyanobacteria.

    Kitayama, Y., Nishiwaki, T., Terauchi, K., Kondo, T.

    Genes and Development   Vol. 22   page: 1513-1521   2008

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  19. シアノバクテリア概日時計蛋白質KaiCのリン酸化

    大川(西脇)妙子

    生化学   Vol. 80   page: 833-838   2008

     More details

    Language:Japanese  

  20. A sequential program of dual phosphorylation of KaiC as a basis for circadian rhythm in cyanobacteria. Reviewed

    Nishiwaki, T., Satomi, Y., Kitayama, Y., Terauchi, K., Kiyohara, R., Takao, T., Kondo, T.

      Vol. 26   page: 4029-4037   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  21. ATPase activity of KaiC determines the basic timing for circadian clock of cyanobacteria. Reviewed

    Terauchi, K., Kitayama, Y., Nishiwaki, T., Miwa, K., Murayama, Y., Oyama, T., Kondo, T.

    Proceedings of National Academy of Sciences of USA   Vol. 104   page: 16377-16381   2007

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  22. Cyanobacterial circadian pacemaker: Kai protein complex dynamics in the KaiC phosphorylation cycle in vitro. Reviewed

    Kageyama H., Nishiwaki, T., Nakajima, M., Iwasaki, H., Oyama, T., Kondo, T.

    Molecular Cell   Vol. 23   page: 161-171   2006

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  23. シアノバクテリアの概日時計システム−時計遺伝子の転写・翻訳フィードバック制御とリン酸化振動のリズム Reviewed

    大川(西脇)妙子、岩崎秀雄

    医学のあゆみ   Vol. 216   page: 238-242   2006

     More details

    Language:Japanese  

  24. Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro. Reviewed

    Nakajima, M., Imai, K., Ito, H., Nishiwaki, T., Murayama, Y., Iwasaki, H., Oyama, T. and Kondo T.

    Science   Vol. 308   page: 414-415   2005

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  25. Role of KaiC phosphorylation in the circadian clock system of Synechococcus elongatus PCC7942 Reviewed

    Nishiwaki, T., Satomi, Y., Nakajima, M., Lee, C., Kiyohara, R., Kageyama, H., Kitayama, Y., Temamoto, M., Yamaguchi, A., Hijikata, A., Go, M., Iwasaki, H., Takao, T., Kondo T.

    Proceedings of National Academy of Sciences of USA   Vol. 101   page: 13927-13932   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  26. Circadian rhythms in the synthesis and degradation of a master clock protein KaiC in cyanobacteria. Reviewed

    Imai, K., Nishiwaki, T., Kondo, T. and Iwasaki, H.

    The Journal of Biological Chemistry   Vol. 279   page: 36534-36539   2004

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  27. KaiB functions as an attenuator of KaiC phosphorylation in the cyanobacterial circadian clock system. Reviewed

    Kitayama, Y., Iwasaki, H. Nishiwaki, T. and Kondo, T.

    EMBO Journal   Vol. 22   page: 2127-2134   2003

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  28. KaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteria.

    Iwasaki, H., Nishiwaki, T. , Kitayama, Y., Nakajima, M, and Kondo, T.

    Proceedings of National Academy of Sciences of USA   Vol. 99   page: 15788-15793   2002

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  29. Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria. Reviewed

    Nishiwaki, T., Iwasaki, H., Ishiura, M. and Kondo, T.

    Proceedings of National Academy of Sciences of USA   Vol. 97   page: 495-499   2000

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  30. Characterizaion and developmental regulation of proteoglycan-type protein tyrosine phosphatase ζ/ RPTPβ isoforms. Reviewed

    Nishiwaki, T., Maeda, N., Noda, M.

    Journal of Biochemistry   Vol. 130   page: 458-467   1998

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  31. Characterization of rat receptor-like protein tyrosine phosphatase γ isoforms. Reviewed

    Shintani, T., Maeda, N., Nishiwaki, T., Noda, M.

    Biochemical and Biophysical Research communications   Vol. 239   page: 410-425   1997

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  32. 6B4 proteoglycan/ phosphacan, and extracellular variant of receptor-like protein-tyrosine phosphatase ζ/RPTPβ, binds pleiotrophin/ heparin-binding growth associated molecule (HB-GAM). Reviewed

    Maeda, N., Nishiwaki, T., Shintani, T., Hamanaka, H. and Noda, M.

    The Journal of BIological Chemistry   Vol. 271   page: 21446-21452   1996

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  33. Differences of somatostatin mRNA in the rat suprachiasmatic nucleus under light-dark and constant dark conditions: an analysis by in situ hybridization. Reviewed

    Nishiwaki, T., Okamura, H., Kanemasa, K., Inatomi, T., Ibata, Y., Fukuhara, C., Inouye, S-I.T.

    Neuroscience Letters   Vol. 197   page: 231-234   1995

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  34. Circadian change of VIP mRNA in the rat suprachiasmatic nucleus following p-chlorophenylalanine (PCPA) treatment in constant darkness. Reviewed

    Okamura, H., Kawakami, F., Tamada, Y., Geffard, M., Nishiwaki, T., Ibata, Y. Inouye, S-I.T.

    Molecular Brain Research   Vol. 29   page: 358-364   1995

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  35. Multiple receptor-like protein tyrosine phosphatases in the form of chondroitin sulfate proteoglycan. Reviewed

    Maeda, N., Hamanaka, H., Shintani, T., Nishiwaki, T., Noda, M.

    FEBS Letters   Vol. 354   page: 67-70   1994

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  36. Emergence of VIP rhythmicity following somatostatin depletion in the rat suprachiasmatic nucleus. Reviewed

    Fukuhara, C., Nishiwaki, T., Cagampang, F.R.A., Inouye, S-I.T.

    Brain Research   Vol. 645   page: 343-346   1994

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

▼display all

Books 1

  1. Circadian Rhythms in Bacteria and Microbiomes Reviewed International journal

    Taeko Nishiwaki-Ohkawa( Role: Joint author ,  Roles of Phosphorylation of KaiC in the Cyanobacterial Circadian Clock.)

    Springer  2021.6  ( ISBN:978-3-030-72158-9

     More details

    Responsible for pages:241-158   Language:English Book type:Scholarly book

    DOI: 10.1007/978-3-030-72158-9_13

Presentations 3

  1. BMAL1の恒常発現レベルは概日リズムの頑健性を調節する

    Apirada PADLOM, 濱島李旺, 小野大輔, 古川祐子, 吉村崇, 大川(西脇)妙子

    第29回日本時間生物学会学術大会  2022.12.3  日本時間生物学会

     More details

    Event date: 2022.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:宇都宮大学  

  2. 概日リズムを制御する化合物の構造活性相関解析

    長野 凌、 永田 晃弘、 鈴木 隆平、 三好 隆也、 東 祐佳、佐藤 綾人、 大松 亨介、大井 貴史、吉村 崇、大川 妙子

    第29回日本時間生物学会学術大会  2022.12.3  日本時間生物学会

     More details

    Event date: 2022.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:宇都宮大学  

  3. Levels of constitutively expressed Bmal1 affect the robustness of circadian oscillation

    Apirada PADLOM, Rio HAMASHIMA, Daisuke ONO, Yuko FURUKAWA, Takashi YOSHIMURA, Taeko OHKAWA

    2021.11.20 

     More details

    Event date: 2021.11

    Language:English  

    Country:Japan  

KAKENHI (Grants-in-Aid for Scientific Research) 6

  1. 過渡応答解析による概日時計の転写・翻訳フィードバックループモデルの再検討

    Grant number:23K05751  2023.4 - 2026.3

    科学研究費助成事業  基盤研究(C)

    西脇妙子

      More details

    Authorship:Principal investigator 

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    睡眠覚醒、体温、ホルモン分泌などの様々な生理活性は、約24時間周期の概日リズムを示すことが知られている。このリズムは生物が昼夜の環境変動に適応するために重要であり、体内時計(概日時計)によって駆動されている。概日時計のリズム発振機構は、概日時計遺伝子産物(概日時計タンパク質)が自身をコードするmRNAの転写を抑制する、転写・翻訳のネガティブフィードバックループモデルにより理解されているが、近年このモデルを支持しない現象も報告されている。またこのモデルは、リズムの周期がなぜ約24時間になるかは十分に説明できない。本研究では定量的な実験と数値解析によりモデルの検証と周期決定機構の解明に取り組む。

  2. Understanding the seasonal adaptation mechanism and its application

    Grant number:19H05643  2019.6 - 2024.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

      More details

    Authorship:Coinvestigator(s) 

  3. Development of new target discovery methods for circadian clock regulating compounds and elucidation of period and phase control mechanisms

    Grant number:19H03178  2019.4 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Ohkawa Taeko

      More details

    Authorship:Principal investigator 

    Grant amount:\17810000 ( Direct Cost: \13700000 、 Indirect Cost:\4110000 )

    For target identification by genetic methods, a genome-wide knockout library was introduced into a human diploid cell line, and the rhythms of approximately 3,000 clones were measured in 384-well plates. As a result, changes in the period and amplitude of the circadian rhythm were observed in about 0.5% of the clones. Using this system, target of the circadian clock-modulating compound can be identified by isolating clones whose response to the compound differs from that of wild-type cells. This screening system can also be used to identify novel circadian clock genes.
    By derivatization of picrotoxinin, a compound that shortens the circadian rhythm cycle, we have obtained a compound that shows approximately 1,000-fold higher activity. We are going to perform affinity purification using the new derivative to identify the target.

  4. Investigation for novel circadian clock reactions independent of the transcription-translation feedback loop of clock genes

    Grant number:15H04349  2015.4 - 2018.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Take Ohkawa

      More details

    Authorship:Principal investigator 

    Grant amount:\16380000 ( Direct Cost: \12600000 、 Indirect Cost:\3780000 )

    The widely accepted models for circadian clocks are based on transcriptional-translational negative feedback loops composed of circadian clock genes. However, some important aspects, such as how the periods are adjusted around 24 h and how the phase of the rhythms are determined, are not fully incorporated into these models. On the other hand, there are several examples of protein posttranscriptional modification rhythms independent of the feedback loops, though their exact roles in the circadian clock systems are still unknown. In this study we tried to elucidate these points by proteomic and chemical biology approach. We obtained compounds that affect period and phase and the potential candidates of their target proteins. We also set up the system to study posttranslational modification rhythms including phosphorylation. By these bidirectional approaches, we are now trying to understand the whole circadian clock systems.

  5. 赤血球前駆細胞を利用した転写翻訳に依存しない概日リズム発振機構の解明

    2014.4 - 2017.3

    科学研究費補助金  挑戦的萌芽研究

    大川(西脇)妙子

      More details

    Authorship:Principal investigator 

  6. ATPを中間体とする概日時計蛋白質KaiCの新規自己脱リン酸化機構とその意義

    2011.4 - 2013.3

    科学研究費補助金  新学術領域研究「水を主役としたATPエネルギー変換」公募研究

      More details

    Authorship:Principal investigator 

▼display all

Industrial property rights 2

  1. 植物細胞分裂抑制剤

    法人名大

     More details

    Date applied:2016.2

    Announcement no:2017-145218  Date announced:2017.8

    Country of applicant:Domestic   Country of acquisition:Domestic

  2. タンパク質を含む時間計測用組成物およびその利用

    近藤孝男, 小山時隆, 岩崎秀雄, 中島正人, 大川妙子 

     More details

    Applicant:国立大学法人 名古屋大学, 独立行政法人 科学技術振興機構

    Application no:2005-300920  Date applied:2005.10

    Announcement no:2007-104998 

    Patent/Registration no:特許第4939791号  Date registered:2012.3 

    Country of applicant:Domestic  

 

Teaching Experience (On-campus) 23

  1. Laboratory in Biology

    2020

  2. 動物生理学特論1

    2020

  3. The World of Genes

    2020

  4. Laboratory and Practice on Bioresource Sciences 2

    2020

  5. Laboratory and Practice on Bioresource Sciences 1

    2020

  6. Cell Technology

    2020

  7. Basic Laboratory and Practice in Bioresource Sciences

    2020

  8. Animal Physiology 1

    2020

  9. 生命農学演習4

    2020

  10. 生命農学演習3

    2020

  11. 生命農学演習2

    2020

  12. 生命農学演習1

    2020

  13. 修士論文研究4

    2020

  14. 修士論文研究3

    2020

  15. 修士論文研究2

    2020

  16. 修士論文研究1

    2020

  17. The world of genes

    2020

  18. 動物生理学1

    2018

  19. 細胞工学

    2018

  20. 資源生物科学基盤実験実習

    2018

  21. 動物生理学特論1

    2018

  22. 生物学実験

    2018

  23. 遺伝子の世界

    2018

▼display all