Updated on 2024/03/28
博士（理学） （ 1998.3 総合研究大学院大学 ）
Life Science / Functional biochemistry
Others / Others / Chronobiology
Life Science / Animal physiological chemistry, physiology and behavioral biology
The Graduate University for Advanced Studies Graduate School, Division of Life Science
- 1998.3
Country： Japan
日本時間生物学会 評議員 編集委員
日本生化学会
日本生物物理学会
東海畜産学会 評議員
2017.4
Level of constitutively expressed BMAL1 affects the robustness of circadian oscillations Reviewed
Padlom, A; Ono, D; Hamashima, R; Furukawa, Y; Yoshimura, T; Nishiwaki-Ohkawa, T
SCIENTIFIC REPORTS Vol. 12 ( 1 ) page： 19519 2022.11
Regulation mechanisms of the dual ATPase in KaiC Reviewed
Furuike, Y; Mukaiyama, A; Koda, SI; Simon, D; Ouyang, DY; Ito-Miwa, K; Saito, S; Yamashita, E; Nishiwaki-Ohkawa, T; Terauchi, K; Kondo, T; Akiyama, S
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Vol. 119 ( 19 ) page： e2119627119 2022.5
Modulation of circadian clock by crude drug extracts used in Japanese Kampo medicine
Zhang, MH; Kobayashi, K; Atsumi, H; Katada, Y; Nakane, Y; Chen, JF; Nagano, R; Kadofusa, N; Nishiwaki-Ohkawa, T; Kon, N; Hirota, T; Sato, A; Makino, T; Yoshimura, T
SCIENTIFIC REPORTS Vol. 11 ( 1 ) page： 21038 2021.10
Okimura, K; Nakane, Y; Nishiwaki-Ohkawa, T; Yoshimura, T
SCIENTIFIC REPORTS Vol. 11 ( 1 ) page： 1843 2021.1
Seasonal changes in NRF2 antioxidant pathway regulates winter depression-like behavior
Nakayama, T; Okimura, K; Shen, J; Guh, YJ; Tamai, TK; Shimada, A; Minou, S; Okushi, Y; Shimmura, T; Furukawa, Y; Kadofusa, N; Sato, A; Nishimura, T; Tanaka, M; Nakayama, K; Shiina, N; Yamamoto, N; Loudon, AS; Nishiwaki-Ohkawa, T; Shinomiya, A; Nabeshima, T; Nakane, Y; Yoshimura, T
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Vol. 117 ( 17 ) page： 9594 - 9603 2020.4
Casein kinase 1 family regulates PRR5 and TOC1 in the Arabidopsis circadian clock
Uehara, TN; Mizutani, Y; Kuwata, K; Hirota, T; Sato, A; Mizoi, J; Takao, S; Matsuo, H; Suzuki, T; Ito, S; Saito, AN; Nishiwaki-Ohkawa, T; Yamaguchi-Shinozaki, K; Yoshimura, T; Kay, SA; Itami, K; Kinoshita, T; Yamaguchi, J; Nakamichi, N
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Vol. 116 ( 23 ) page： 11528 - 11536 2019.6
Identification of circadian clock modulators from existing drugs
Tamai T. Katherine, Nakane Yusuke, Ota Wataru, Kobayashi Akane, Ishiguro Masateru, Kadofusa Naoya, Ikegami Keisuke, Yagita Kazuhiro, Shigeyoshi Yasufumi, Sudo Masaki, Nishiwaki-Ohkawa Taeko, Sato Ayato, Yoshimura Takashi
EMBO MOLECULAR MEDICINE Vol. 10 ( 5 ) 2018.5
Combination of synthetic chemistry and live-cell imaging identified a rapid cell division inhibitor in Tobacco and Arabidopsis thaliana Reviewed
Nambo M, Kurihara D, Yamada T, Nishiwaki-Ohkawa T, Kadofusa N, Kimata Y, Kuwata K, Umeda M, Ueda M
Plant and Cell Physiology Vol. 57 ( 11 ) page： 2255-2268 2016.8
Molecular basis for regulating seasonal reproduction in vertebrates Invited Reviewed
Nishiwaki-Ohkawa T, Yoshimura T
Journal of Endocrinology Vol. 229 page： R117-R127 2016.4
Electrochemical Detection of Circadian redox rhythm in cyanobacterial cells via extracellular electron transfer. Reviewed
1. Nishio, K., Pornpitra, T., Izawa, S., Nishiwaki-Ohkawa, T., Kato, S., Hashimoto, K., and Nakanishi, S.
Plant Cell Physiology 2015.5
C-H activation generates period-shortening molecules that target Cryptochrome in the mammalian circadian clock. Reviewed
Oshima, T., Yamanaka, I., Kumar, A., Yamaguchi, J., Nishiwaki-Ohkawa, T., Muto, K., Kawamura, R., Hirota T., Yagita, K., Irle, S., Kay, S.A., Yoshimura, T., and Itami, K.
Angewandte Chemie International Edition 2015.5
Tissue-specific post-translational modification allows functional targeting of thyrotropin. Reviewed
Ikegami, K., Liao, X.-H., Hoshino, Y., Ono, H., Ota, W., Ito, Y., Nishiwaki-Ohkawa, T., Sato, C., Kitajima, K., Iigo, M., Shigeyoshi Y., Yamada, M., Murata, Y., Refetoff, S and Yoshimura T.
Cell Reports Vol. 9 ( 3 ) page： 801-809 2014.11
Exchange of ADP with ATP in the CII ATPase domain promotes autophosphorylation of cyanobacterial clock protein KaiC Reviewed
Taeko Nishiwaki-Ohkawa, Yohko Kitayama, Erika Ochiai, and Takao Kondo
Proceedings of National Academy of Sciences of USA Vol. 111 page： 4455-4460 2014.3
Regulation of seasonal reproduction by hypothalamic activation of thyroid hormone. Reviewed
Shinomiya A., Shimmura T., Nishiwaki-Ohkawa, T., Yoshimura T
Frontiers in Endocrinology 2014.2
Intersubunit communications within KaiC hexamers contribute the robust rhythmicity of the cyanobacterial circadian clock. Reviewed
Kitayama Y., Nishiwaki-Ohkawa T., Kondo T.
Microbial Cell Vol. 1 page： 67-69 2014.1
KaiC intersubunit communication facilitates robustness of circadian rhythm in cyanobacteria Reviewed
Kitayama Y., Nishiwaki-Ohkawa, T., Sugisawa Y. Kondo T
Nature Communications 2013.12
Circadian autodephosphorylation of cyanobacterial clock protein KaiC occurs via formation of ATP as intermediate Reviewed
Nishiwaki, T., Kondo, T.
The Journal of Biological Chemistry Vol. 287 page： 18030-18035 2012
Dual KaiC-based oscillations constitute the circadian system of cyanobacteria.
Kitayama, Y., Nishiwaki, T., Terauchi, K., Kondo, T.
Genes and Development Vol. 22 page： 1513-1521 2008
シアノバクテリア概日時計蛋白質KaiCのリン酸化
大川(西脇)妙子
生化学 Vol. 80 page： 833-838 2008
A sequential program of dual phosphorylation of KaiC as a basis for circadian rhythm in cyanobacteria. Reviewed
Nishiwaki, T., Satomi, Y., Kitayama, Y., Terauchi, K., Kiyohara, R., Takao, T., Kondo, T.
Vol. 26 page： 4029-4037 2007
ATPase activity of KaiC determines the basic timing for circadian clock of cyanobacteria. Reviewed
Terauchi, K., Kitayama, Y., Nishiwaki, T., Miwa, K., Murayama, Y., Oyama, T., Kondo, T.
Proceedings of National Academy of Sciences of USA Vol. 104 page： 16377-16381 2007
Cyanobacterial circadian pacemaker: Kai protein complex dynamics in the KaiC phosphorylation cycle in vitro. Reviewed
Kageyama H., Nishiwaki, T., Nakajima, M., Iwasaki, H., Oyama, T., Kondo, T.
Molecular Cell Vol. 23 page： 161-171 2006
シアノバクテリアの概日時計システム−時計遺伝子の転写・翻訳フィードバック制御とリン酸化振動のリズム Reviewed
大川(西脇)妙子、岩崎秀雄
医学のあゆみ Vol. 216 page： 238-242 2006
Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro. Reviewed
Nakajima, M., Imai, K., Ito, H., Nishiwaki, T., Murayama, Y., Iwasaki, H., Oyama, T. and Kondo T.
Science Vol. 308 page： 414-415 2005
Role of KaiC phosphorylation in the circadian clock system of Synechococcus elongatus PCC7942 Reviewed
Nishiwaki, T., Satomi, Y., Nakajima, M., Lee, C., Kiyohara, R., Kageyama, H., Kitayama, Y., Temamoto, M., Yamaguchi, A., Hijikata, A., Go, M., Iwasaki, H., Takao, T., Kondo T.
Proceedings of National Academy of Sciences of USA Vol. 101 page： 13927-13932 2004
Circadian rhythms in the synthesis and degradation of a master clock protein KaiC in cyanobacteria. Reviewed
Imai, K., Nishiwaki, T., Kondo, T. and Iwasaki, H.
The Journal of Biological Chemistry Vol. 279 page： 36534-36539 2004
KaiB functions as an attenuator of KaiC phosphorylation in the cyanobacterial circadian clock system. Reviewed
Kitayama, Y., Iwasaki, H. Nishiwaki, T. and Kondo, T.
EMBO Journal Vol. 22 page： 2127-2134 2003
KaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteria.
Iwasaki, H., Nishiwaki, T. , Kitayama, Y., Nakajima, M, and Kondo, T.
Proceedings of National Academy of Sciences of USA Vol. 99 page： 15788-15793 2002
Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria. Reviewed
Nishiwaki, T., Iwasaki, H., Ishiura, M. and Kondo, T.
Proceedings of National Academy of Sciences of USA Vol. 97 page： 495-499 2000
Characterizaion and developmental regulation of proteoglycan-type protein tyrosine phosphatase ζ/ RPTPβ isoforms. Reviewed
Nishiwaki, T., Maeda, N., Noda, M.
Journal of Biochemistry Vol. 130 page： 458-467 1998
Characterization of rat receptor-like protein tyrosine phosphatase γ isoforms. Reviewed
Shintani, T., Maeda, N., Nishiwaki, T., Noda, M.
Biochemical and Biophysical Research communications Vol. 239 page： 410-425 1997
6B4 proteoglycan/ phosphacan, and extracellular variant of receptor-like protein-tyrosine phosphatase ζ/RPTPβ, binds pleiotrophin/ heparin-binding growth associated molecule (HB-GAM). Reviewed
Maeda, N., Nishiwaki, T., Shintani, T., Hamanaka, H. and Noda, M.
The Journal of BIological Chemistry Vol. 271 page： 21446-21452 1996
Differences of somatostatin mRNA in the rat suprachiasmatic nucleus under light-dark and constant dark conditions: an analysis by in situ hybridization. Reviewed
Nishiwaki, T., Okamura, H., Kanemasa, K., Inatomi, T., Ibata, Y., Fukuhara, C., Inouye, S-I.T.
Neuroscience Letters Vol. 197 page： 231-234 1995
Circadian change of VIP mRNA in the rat suprachiasmatic nucleus following p-chlorophenylalanine (PCPA) treatment in constant darkness. Reviewed
Okamura, H., Kawakami, F., Tamada, Y., Geffard, M., Nishiwaki, T., Ibata, Y. Inouye, S-I.T.
Molecular Brain Research Vol. 29 page： 358-364 1995
Multiple receptor-like protein tyrosine phosphatases in the form of chondroitin sulfate proteoglycan. Reviewed
Maeda, N., Hamanaka, H., Shintani, T., Nishiwaki, T., Noda, M.
FEBS Letters Vol. 354 page： 67-70 1994
Emergence of VIP rhythmicity following somatostatin depletion in the rat suprachiasmatic nucleus. Reviewed
Fukuhara, C., Nishiwaki, T., Cagampang, F.R.A., Inouye, S-I.T.
Brain Research Vol. 645 page： 343-346 1994
Circadian Rhythms in Bacteria and Microbiomes Reviewed International journal
Taeko Nishiwaki-Ohkawa（ Role： Joint author , Roles of Phosphorylation of KaiC in the Cyanobacterial Circadian Clock.）
Springer 2021.6 （ ISBN:978-3-030-72158-9 ）
BMAL1の恒常発現レベルは概日リズムの頑健性を調節する
Apirada PADLOM, 濱島李旺, 小野大輔, 古川祐子, 吉村崇, 大川（西脇）妙子
第29回日本時間生物学会学術大会 2022.12.3 日本時間生物学会
概日リズムを制御する化合物の構造活性相関解析
長野 凌、 永田 晃弘、 鈴木 隆平、 三好 隆也、 東 祐佳、佐藤 綾人、 大松 亨介、大井 貴史、吉村 崇、大川 妙子
第29回日本時間生物学会学術大会 2022.12.3 日本時間生物学会
Levels of constitutively expressed Bmal1 affect the robustness of circadian oscillation
Apirada PADLOM, Rio HAMASHIMA, Daisuke ONO, Yuko FURUKAWA, Takashi YOSHIMURA, Taeko OHKAWA
2021.11.20
過渡応答解析による概日時計の転写・翻訳フィードバックループモデルの再検討
Grant number：23K05751 2023.4 - 2026.3
科学研究費助成事業 基盤研究(C)
西脇妙子
Authorship：Principal investigator
Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）
睡眠覚醒、体温、ホルモン分泌などの様々な生理活性は、約24時間周期の概日リズムを示すことが知られている。このリズムは生物が昼夜の環境変動に適応するために重要であり、体内時計（概日時計）によって駆動されている。概日時計のリズム発振機構は、概日時計遺伝子産物（概日時計タンパク質）が自身をコードするmRNAの転写を抑制する、転写・翻訳のネガティブフィードバックループモデルにより理解されているが、近年このモデルを支持しない現象も報告されている。またこのモデルは、リズムの周期がなぜ約24時間になるかは十分に説明できない。本研究では定量的な実験と数値解析によりモデルの検証と周期決定機構の解明に取り組む。
Understanding the seasonal adaptation mechanism and its application
Grant number：19H05643 2019.6 - 2024.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (S)
Authorship：Coinvestigator(s)
Grant number：19H03178 2019.4 - 2022.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Ohkawa Taeko
Authorship：Principal investigator
Grant amount：\17810000 （ Direct Cost: \13700000 、 Indirect Cost：\4110000 ）
For target identification by genetic methods, a genome-wide knockout library was introduced into a human diploid cell line, and the rhythms of approximately 3,000 clones were measured in 384-well plates. As a result, changes in the period and amplitude of the circadian rhythm were observed in about 0.5% of the clones. Using this system, target of the circadian clock-modulating compound can be identified by isolating clones whose response to the compound differs from that of wild-type cells. This screening system can also be used to identify novel circadian clock genes.
By derivatization of picrotoxinin, a compound that shortens the circadian rhythm cycle, we have obtained a compound that shows approximately 1,000-fold higher activity. We are going to perform affinity purification using the new derivative to identify the target.
Grant number：15H04349 2015.4 - 2018.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Take Ohkawa
Authorship：Principal investigator
Grant amount：\16380000 （ Direct Cost: \12600000 、 Indirect Cost：\3780000 ）
The widely accepted models for circadian clocks are based on transcriptional-translational negative feedback loops composed of circadian clock genes. However, some important aspects, such as how the periods are adjusted around 24 h and how the phase of the rhythms are determined, are not fully incorporated into these models. On the other hand, there are several examples of protein posttranscriptional modification rhythms independent of the feedback loops, though their exact roles in the circadian clock systems are still unknown. In this study we tried to elucidate these points by proteomic and chemical biology approach. We obtained compounds that affect period and phase and the potential candidates of their target proteins. We also set up the system to study posttranslational modification rhythms including phosphorylation. By these bidirectional approaches, we are now trying to understand the whole circadian clock systems.
赤血球前駆細胞を利用した転写翻訳に依存しない概日リズム発振機構の解明
2014.4 - 2017.3
科学研究費補助金 挑戦的萌芽研究
大川（西脇）妙子
Authorship：Principal investigator
ATPを中間体とする概日時計蛋白質KaiCの新規自己脱リン酸化機構とその意義
2011.4 - 2013.3
科学研究費補助金 新学術領域研究「水を主役としたATPエネルギー変換」公募研究
Authorship：Principal investigator
植物細胞分裂抑制剤
法人名大
タンパク質を含む時間計測用組成物およびその利用
近藤孝男, 小山時隆, 岩崎秀雄, 中島正人, 大川妙子
Laboratory in Biology
2020
動物生理学特論1
2020
The World of Genes
2020
Laboratory and Practice on Bioresource Sciences 2
2020
Laboratory and Practice on Bioresource Sciences 1
2020
Cell Technology
2020
Basic Laboratory and Practice in Bioresource Sciences
2020
Animal Physiology 1
2020
修士論文研究４
2020
修士論文研究３
2020
修士論文研究２
2020
修士論文研究１
2020
生命農学演習４
2020
生命農学演習３
2020
生命農学演習２
2020
生命農学演習１
2020
The world of genes
2020
動物生理学1
2018
細胞工学
2018
資源生物科学基盤実験実習
2018
動物生理学特論1
2018
生物学実験
2018
遺伝子の世界
2018
