Updated on 2024/03/26

写真a

 
YOSHIOKA, Yasushi
 
Organization
Graduate School of Science Associate professor
Graduate School
Graduate School of Science
Undergraduate School
School of Science
Title
Associate professor
Contact information
メールアドレス

Degree 1

  1. 農学博士 ( 1989.3   東京大学 ) 

Research Interests 3

  1. plastid division

  2. plant morphogenesis

  3. 色素体機能

Research Areas 1

  1. Others / Others  / Plant Physiology/Molecule

Education 2

  1. The University of Tokyo   Graduate School, Division of Agricultural Science

    - 1989

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    Country: Japan

  2. The University of Tokyo   Faculty of Agriculture

    - 1984

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    Country: Japan

Professional Memberships 3

  1. 日本植物生理学会   評議員

    2010.4 - 2012.3

  2. 日本植物学会

  3. 日本分子生物学会

 

Papers 28

  1. CRUMPLED LEAF supports plastid OUTER ENVELOPE PROTEIN OF 80 KDA complex formation in Arabidopsis

    Yoshimura, R; Minamikawa, S; Suzuki, T; Goto, K; Latrasse, D; Sicar, S; Raynaud, C; Benhamed, M; Yoshioka, Y

    PLANT PHYSIOLOGY     2024.1

  2. Application of CRISPR/Cas9 genome editing system for molecular breeding of orchids. Reviewed International coauthorship

    Endang Semiarti, Sri Nopitasari, Yuli Setiawati, Muhammad Dylan Lawrie, Aziz Purwantoro, Jaka Widada, Kana Ninomiya, Yuuki Asano, Shogo Matsumoto, Yasushi Yoshioka

    Indonesian Journal of Biotechnology   Vol. 25 ( 1 ) page: 61 - 68   2020.5

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    Authorship:Last author   Language:English  

    DOI: 10.22146/ijbiotech.39485

  3. Agrobacterium-mediated Transformation Facillitates the CRISPR/Cas9 Genome Editing System in Dendrobium macrophyllum A. Rich Orchid Reviewed International coauthorship

    6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE (ICBS 2019) - BIODIVERSITY AS A CORNERSTONE FOR EMBRACING FUTURE HUMANITY   Vol. 2260   2020

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    Language:English   Publishing type:Research paper (international conference proceedings)  

    DOI: 10.1063/5.0016200

    Web of Science

  4. Development of an Agrobacterium-delivered CRISPR/Cas9 for Phalaenopsis amabilis (L.) Blume Genome Editing System Reviewed International coauthorship

    6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE (ICBS 2019) - BIODIVERSITY AS A CORNERSTONE FOR EMBRACING FUTURE HUMANITY   Vol. 2260   2020

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    Language:English   Publishing type:Research paper (international conference proceedings)  

    DOI: 10.1063/5.0015868

    Web of Science

  5. Chloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis crumpled leaf Mutant Reviewed

    Elodie Hudik, Yasushi Yoshioka, Severine Domenichini, Mickael Bourge, Ludivine Soubigout-Taconnat, Christelle Mazubert, Dalong Yi, Sandrine Bujaldon, Hiroyuki Hayashi, Lieven De Veylder, Catherine Bergounioux, Moussa Benhamed, and Cecile Raynaud

    Plant Physiology   Vol. 166   page: 152-167   2014.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1104/pp.114.242628

  6. Lacking chloroplasts in guard cells of crumpled leaf attenuates stomatal opening: both guard cell chloroplasts and mesophyll contribute to guard cell ATP levels. Reviewed

    Shu-Wei Wang, Ying Li, Xiao-Lu Zhang, Hai-Qiang Yang, Xue-Fei Han Zhao-Hui Liu, Zhong-Lin Shang, Tomoya Asano, Yasushi Yoshioka, Chun-Guang Zhang and Yu-Ling Chen.

    Plant, Cell and Environment   Vol. 37   page: 2201-2210   2014.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: Lacking chloroplasts in guard cells of crumpled leaf attenuates

  7. Visualization of plastid movement in the pollen tube of Arabidopsis thaliana Reviewed

    Makoto T. Fujiwara, Yasushi Yoshioka, Tomonari Hirano, Yusuke Kazama, Tomoko Abe, Kensuke Hayashi, Ryuuichi D. Itoh

    Plant Signaling & Behavior   Vol. 7 ( 1 ) page: 1-4   2012.1

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    Language:English   Publishing type:Research paper (scientific journal)  

  8. CRUMPLED LEAF (CRL) Homologs of Physcomitrella patens are Involved in the Complete Separation of Dividing Plastids. Reviewed

    Sugita, C., Kato, Y., Yoshioka, Y., Tsurumi, N., Iida, Y., Machida, Y., Sugita, M.

    Plant Cell Physiol.   Vol. 53   page: 1124-1133   2012

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    Language:English   Publishing type:Research paper (scientific journal)  

    Plastid division is controlled by numerous nuclear genes. Arabidopsis thaliana CRUMPLED LEAF (AtCRL) is a plastid division-related gene, and the crl mutant exhibits a dwarf phenotype with abnormal cell division and a significant re- duction in plastid numbers. However, the function of AtCRL is not fully understood. Here, we identified and character- ized two AtCRL homologs, PpCRL1 and PpCRL2, in the moss Physcomitrella patens. PpCRL1 and PpCRL2 shared 77% amino acid identity with each other and 47% identity with AtCRL. Single PpCRL1 or -2 gene knockout (KO) mutants could not be distinguished from the wild-type mosses, but PpCRL1 and -2 double KO mutants displayed growth retard- ation of protonemata and gametophores and harbored ap- proximately 10 large chloroplasts per cell. This indicates that PpCRL1 and PpCRL2 have redundant functions in chloroplast division and plant growth. Unlike the A. thaliana crl mu- tants, however, the PpCRL double KO mutants did not dis- play abnormal orientation of the cell division plane. Complementation experiments showed that AtCRL partially rescued the defects in chloroplast size and number of the PpCRL double KO mutant. This suggests that PpCRL has a similar, but not identical, function to AtCRL. Time-lapse microscopic observation of the double PpCRL KO mutants revealed that some dumbbell-shaped chloroplasts failed to complete division at the late stage of plastid division; enlarged chloroplasts were thus generated. This strongly suggests that PpCRLs are involved in the complete separ- ation of dividing chloroplasts.

  9. Role of GIG1 and UVI4 genome duplication in Arabidopsis thaliana. Reviewed

    Iwata, E., Ikeda S., Abe, N., Kobayashi, A., Kurata, M., Mtsunaga, S., Yoshioka, Y., Criqui, M.-C., Genschik, P., Ito, M

    Plant Signaling Behavior   Vol. 7   page: 1079–1081   2012

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    Language:English   Publishing type:Research paper (scientific journal)  

    Endomitosis and endoreplication are atypical modes of cell cycle that results in genome duplication in single nucleus. Because the cell size of given cell type is generally proportional to the nuclear DNA content, endoreplication and endomitosis are effective strategy of cell growth, which are widespread in multicellular organisms, especially those in plant kingdom. We found that these processes might be differently regulated by GIGAS CELL1 (GIG1) and its paralog UV-INSENSITIVE4 (UVI4) in Arabidopsis thaliana. GIG1 and UVI4 may negatively regulate activities of anaphase-promoting complex or cyclosome (APC/C) ubiquitin ligase that acts as an important mitotic regulator. The gig1 mutation induced ectopic occurrence of endomitosis during somatic cell division, while it has been reported that uvi4 mutation resulted in premature occurrence of endoreplication during organ development. Overexpression of GIG1 and UVI4 dramatically increased the amount of mitotic cyclin, CYCB1;1, a well-known substrate of APC/C. Ectopic endomitosis in gig1 was enhanced by mutation in CYCB2;2 and suppressed by downregulation of APC10 encoding a core subunit of APC/C. Overexpression of CDC20.1, an activator protein of APC/C, further promoted the ectopic endomitosis in gig1. These findings suggest that endomitosis and endoreplication are regulated by similar molecular mechanisms, in which two related proteins, GIG1 and UVI4, may inhibit APC/C in different ways.

  10. GIGAS CELL1, a novel negative regulator of the anaphase-promoting complex/cyclosome, is required for proper mitotic progression and cell fate determination in Arabidopsis thaliana. Reviewed

    Iwata, E., Ikeda S., Mtsunaga, S., Kurata, M., Yoshioka, Y., Criqui, M.-C., Genschik, P., Ito, M

    Plant Cell   Vol. 23   page: 4382–4393   2011

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    Language:English   Publishing type:Research paper (scientific journal)  

    Occurrence of increased cellular ploidy is widespread in developmental processes of multicellular organisms, especially in plants. Elevated ploidy levels are typically achieved either by endoreplication or endomitosis, which are often regarded as modified cell cycle lacking M phase entirely or partially. We have identified GIGAS CELL1 (GIG1)/OMISSION OF SECOND DIVISION1 (OSD1) whose mutation triggered ectopic endomitosis, whereas it has been reported that its paralog, UV-INSENSITIVE4 (UVI4), negatively regulates endoreplication onset in Arabidopsis thaliana. We showed that GIG1/OSD1 and UVI4 encode novel plant-specific inhibitors of anaphase-promoting complex or cyclosome (APC/C) ubiquitin ligase. These proteins physically interact with APC/C activators, CDC20/FZY and CDH1/FZR, in yeast two-hybrid assays. Overexpression of CDC20.1 and CCS52B/FZR3 differentially promoted ectopic endomitosis in gig1/osd1, and premature occurrence of endoreplication in uvi4. Our data suggest that two related proteins, GIG1/OSD1 and UVI4, may prevent unscheduled increase of cellular ploidy by preferentially inhibiting APC/CCDC20 and APC/CFZR, respectively. Generation of cells with a mixed identity in gig1/osd1 further suggested that APC/C may have an unexpected role for cell fate determination in addition to its role for proper mitotic progression.

  11. Dynamic morphologies of pollen plastids visualised by vegetative-specific FtsZ1–GFP in Arabidopsis thaliana Reviewed

    Fujiwara, M.T., Hashimoto, H., Kazama. Y., Hirano, T., Yoshioka, Y., Aoki, S., Sato, N., Itoh, R.D., Abe, T.

    Protoplasma   Vol. 242   page: 19–33   2010

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  12. *Plant cells without detectable plastids are generated in the crumpled leaf mutant of Arabidopsis thaliana. Reviewed

    Chen, Y., Asano, T., Fujiwara, M., Yoshida, S., Machida, Y., Yoshioka, Y.

    Plant Cell Physiol.   Vol. 50   page: 956-969   2009

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  13. Visualization of Plastids in Pollen Grains: Involvement of FtsZ1 in Pollen Plastid Division. Reviewed

    Tang, L.-Y.,Nagata, N., Matsushima, R., Chen, Y., Yoshioka, Y., Sakamoto, W.

    Plant Cell Physiol.   Vol. 50   page: 904-908   2009

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  14. *EMBRYO YELLOW gene, encoding a subunit of the conserved oligomeric Golgi complex, is required for appropriate cell expansion and meristem organization in Arabidopsis thaliana Reviewed

    Ishikawa,T., Machida,C., Yoshioka,Y., Ueda,T., Nakano,A., Machida, Y.

    Genes to Cells   Vol. 13   page: 521–535   2008

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  15. A revised model for the nuclear control of plastid division

    Fujiwara,M.T., Itoh,R.D., Yoshioka,Y.

    Adaptive Gene Regulations - From Microorganisms to Organelles     page: 59-74   2008

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  16. Interaction between Agrobacterium tumefaciens oncoprotein 6b and a tobacco nucleolar protein that is homologous to TNP1 encoded by a transposable element of Antirrhinum majus Reviewed

    Kitakura,S., Terakura,S., Yoshioka,Y., Machida,C., Machida, Y.

    J. Plant Res.   Vol. 121   page: 425-433   2008

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    Language:English   Publishing type:Research paper (scientific journal)  

  17. Agrobacterium-mediated transformation of the wild orchid species Phalaenopsis amabili Reviewed

    Semiarti, E., Indrianto, A., Purwantoro, A., Isminingsih, S., Suseno, N., Ishikawa, T., Yoshioka, Y., Machida, Y., and Machida, C.

    Plant Biotechnology   Vol. 24   page: 265-272   2007

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    Language:English   Publishing type:Research paper (scientific journal)  

  18. Distinct and overlapping roles of two gibberellin 3-oxidases in Arabidopsis development. Reviewed

    Mitchum, M.G., Yamaguchi, S., Hanada, A., Kuwahara, A., Yoshioka, Y., Kato, T., Tabata, S., Kamiy, Y., and Sun, T.-p.

    Plant Journal   Vol. 45   page: 804-818   2006

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    Language:English   Publishing type:Research paper (scientific journal)  

  19. A defect in atToc159 of Arabidopsis thaliana causes severe defects in leaf development. Reviewed

    Asano, T., Yoshioka, Y., Machida, Y.

    Genes & Genetic Systems   Vol. 79   page: 207-212   2004

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  20. Transient Gene Expression in Plant Cells Mediated by Agrobacterium tumefaciens : Application for the Analysis of Virulence Loci Reviewed

    Plant Cell Physiology   Vol. 37 ( 6 ) page: 782-789   1996

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  21. DNA rearrangement associated with the integration of T-DNA in tobacco:an example for multiple duplications of DNA around the integration target Reviewed

    The Plant Journal   Vol. 7 ( 1 ) page: 157-164   1995

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  22. *Molecular characterization of a short interspersed repetitive element from tobacco that exhibits sequence homology to specific tRANs Reviewed

    Proceedings of National Academy of Science U. S. A   Vol. 90   page: 6562-6566   1993

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  23. T-DNAの植物染色体への組込みと遺伝子ターゲティングの試み

    蛋白質核酸酵素   Vol. 37   page: 7   1992

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    Authorship:Lead author   Language:Japanese  

  24. T-DNAの植物染色体組込みに関する最近の知見

    植物細胞工学   Vol. 3   page: 6   1991

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    Authorship:Lead author   Language:Japanese  

  25. Nucleotide sequence of promoter-distal region of the tra operon of plasmid R100, including traI(DNA helicase I)and traD genes. Reviewed

    Journal of Molecular Biology     page: 214   1990

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  26. Identification and characterization of the products from the traJ and traY genes of plasmid R100. Reviewed

    Journal of Bacteriology     page: 170   1988

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  27. *Repressor gene fin0 in plasmid R100 and F : Constitutive transfer of plasmid F is caused by insertion of IS3 into F fin0. Reviewed

    Journal of Bacteriology     page: 169   1987

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  28. Characterization of the gene products produced in minicells by pSM1, a derivative of R100. Reviewed

    Molecular and General Genetics     page: 205   1986

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▼display all

Presentations 30

  1. The CRL protein is involved in the TOC75-V/OEP80 complex formation in Arabidopsis

    Yasushi Yoshioka

    2024.3.17 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

  2. シロイヌナズナCRLは色素体Omp85ファミリータンパク質OEP80の複合体形成に関与する International coauthorship

    吉村 亮

    第63回日本植物生理学会年会  2022.3.23 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  3. Dendrobium formidible花被片プロトプラストを用いた遺伝子一過的発現によるゲノム編集の検出 International coauthorship

    吉岡 泰

    日本植物学会第85回大会  2021.9.16  日本植物学会

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    Event date: 2021.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン   Country:Japan  

  4. シロイヌナズナのcrl変異体のサプレッサー原因遺伝子の同定と解析

    吉村 亮

    第62回日本植物生理学会年会  2021.3.14  日本植物生理学会

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    Event date: 2021.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン  

  5. シロイヌナズナのcrl変異体のサプレッサー系統S2-6の原因遺伝子の同定と解析

    吉村 亮

    第61回日本植物生理学会年会  2020.3.19  日本植物生理学会

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    Event date: 2020.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン  

  6. シロイヌナズナのcrl変異体のサプレッサー系統S1-9の原因遺伝子の同定と解析

    南河 駿

    第61回日本植物生理学会年会  2020.3.19  日本植物生理学会

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    Event date: 2020.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン  

  7. An application of CRISPR/Cas9 genome editing system for molecular breeding of orchids Invited International coauthorship International conference

    The 6th International Conference on Biological Science  2019.10 

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    Event date: 2019.10

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Country:Indonesia  

  8. The establishment of CRISPR/Cas9 genome editing system for Phalaenopsis Orchid: Improvement of transformation methods and transient expression assay International coauthorship International conference

    The 6th International Conference on Biological Science  2019.10 

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    Event date: 2019.10

    Language:English   Presentation type:Poster presentation  

    Country:Indonesia  

  9. Development of an Agrobacterium-delivered CRISPR/Cas9 for Phalaenopsis amabilis (L.) Blume genome editing system International coauthorship International conference

    Sri Nopitasari

    The 6th International Conference on Biological Science  2019.10 

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    Event date: 2019.10

    Language:English   Presentation type:Oral presentation (general)  

    Country:Indonesia  

  10. Agrobacterium-mediated transformation Facillitates the CRISPR/Cas9 genome editing system in Dendrobium macrophyllum A. Rich orchid International coauthorship International conference

    Yuli Setiawat

    The 6th International Conference on Biological Science  2019.10 

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    Event date: 2019.10

    Language:English   Presentation type:Oral presentation (general)  

    Country:Indonesia  

  11. シロイヌナズナcrl変異体のサプレッサー原因遺伝子の同定

    吉村 亮

    第60回日本植物生理学会年会  2019.3.13  日本植物生理学会

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    Event date: 2019.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋大学  

  12. Effect of Sodium Chloride on Chloroplast division in the Moss Physcomitrella paten

    Thi Huong Do

    2019.3.13 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

  13. CRISPR/Cas9システムを用いたファレノプシス属のゲノム編集

    二宮佳奈, 浅野裕己, Endang Semiarti, Aziz Purwantoro, Jaka Widada, Aries Bagus Sasongko, Muhammad Dylan Lawrie, Windi Mose,松本 省吾,吉岡 泰

    日本植物学会第82回大会 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:広島国際会議場   Country:Japan  

  14. シロイヌナズナcrl変異体とヒメツリガネゴケPpCrl1, 2二重遺伝子破壊株おける葉緑体分裂阻害過程の解析

    加藤 優花

    第59回日本植物生理学会年会  2018.3.28  日本植物生理学会

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    Event date: 2018.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋大学  

  15. 環境ストレスに応じた細胞の運命転換と光合成オルガネラ葉緑体の数を制御する新しい制御系

    ポンタイ プラパポーン

    第40回日本分子生物学会年会  2017.12.8 

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    Event date: 2017.12

    Language:English   Presentation type:Oral presentation (general)  

    Venue:神戸市  

  16. Study of chloroplast division of the PpCRL1, 2 double knock out line of Physcomitrella patens using time-lapse live-cell imaging

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    Event date: 2017.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

    PpCRL1 and PpCRL2 genes of Physcomitrella patens function redundantly in progression of chloroplast division and plants' growth. To reveal the role of these genes in chloroplast division, we conducted a time-lapse live-imaging of chloroplast division in Ppcrl1 Ppcrl2 double knock out line (Δ1/2). We observed chloroplasts in gametophores every hour under a confocal microscope and found that chloroplasts in Δ1/2 divided in a similar fashion to wild type. However, divided two chloroplasts were interconnected via a filamentous structure that was visualized by an envelop-localized GFP. We also observed that chloroplasts interconnected via the filamentous structures were fused and became a round shape chloroplast. The frequency of chloroplast fusion in Δ1/2 was 0.13 cell-1 hr-1. Neither filamentous structures nor chloroplast fusion were observed both in wild type and a PpFtsZ2-1 knock out line that is defective in chloroplast division. These results suggest that chloroplasts of Δ1/2 can divide but fail to separate into two daughter chloroplasts, thereby enlarging. PpCRL genes may be involved in the separation process of two daughter chloroplasts.

  17. Effect of ABA on chloroplast division of the moss Physcomitrella patens

    Pongthai Prapaporn, Hiroyoshi Takano, Yasushi Yoshioka, Tomomichi Fujita

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    Event date: 2017.3

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

    Abiotic stresses affect chloroplast division and morphology of chloroplasts in the moss, Physcomitrella patens. Previous data and also our observation showed that ampicillin, an inhibitor of peptidoglycan synthesis, inhibited chloroplast division and resulted in the appearance of macrochloroplasts in the moss. The results suggest that the peptidoglycan synthesis positively regulates chloroplast division. Interestingly, chloroplast division in the bryophytes may be an intermediate between the cyanobacteria and the vascular plants, but the mechanisms are still unclear.
    In this work we examined the relationship between ABA and peptidoglycan synthesis on chloroplast division of the moss, P. patens. We found that chloroplast numbers in the wild type increased under ABA plus ampicillin treatment. Moreover, ABA also increased the number of chloroplasts in the peptidoglycan knockout mutants and the mid-chloroplast FtsZ rings knockout mutant. The results suggest that ABA may act independently of peptidoglycan synthesis pathway and FtsZ to control chloroplast division. To prove our hypothesis and find a novel pathway of chloroplast division, observation of ABA signaling overexpression lines is in progress.

  18. シロイヌナズナCRUMPLED LEAFタンパク質と相互作用するタンパク質の同定と解析

    柴田奨梧,村田綾,青木雄哉,氏原麻衣,野元美佳,多田安臣,吉岡 泰

    第57回日本植物生理学会年会 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:岩手大学(岩手県)   Country:Japan  

    シロイヌナズナのCRUMPLED LEAF (CRL)は、色素体分裂、植物の生長・形態形成に関与する陸上 植物に保存された遺伝子であり、色素体外包膜とミトコンドリアとに局在するタンパク質をコードしている。これまでに我々はin vivoプルダウンアッセイによってCRL-GFPと相互作用するタンパク質候補として、atTOC75-IIIとPROHIBITIN3(PHB3)とを同定した。今回、我々はAlphaScreenによってCRLとPHB3, atTOC75-IIIとの相互作用を確認した。また、atTOC75-III-GFPの過剰発現によってcrl-1の地上部の成長と稔性が部分的に回復する事を見いだした。atTOC75-IIIは葉緑体へのタンパク質輸送に関与する外包膜局在タンパク質であるが、この結果から、atTOC75-IIIの機能低下がcrl-1における稔性低下と成長阻害の原因の1つである可能性が考えられる。PHB3は過酸化水素による一酸化窒素(NO)の生成誘導に関与するミトコンドリア局在タンパク質であり、低濃度のNOは細胞分裂を促進することが報告されている。我々は昨年の本年会において過酸化水素によるNOの生成誘導および内在NO量が、crl-1変異体では野生型に比べて低下していること、および、NO供与剤SNPの添加によって、crl-1の根におけるG2/M期のマーカー遺伝子CYCB1;1-GUS発現が野生型とほぼ同程度に回復する事を報告している。以上の事からcrl-1で観察される成長阻害には葉緑体とミトコンドリアの両方の要因が関与すると考えられる。

  19. シロイヌナズナCRUMPLED LEAF変異体の根の生長に対する一酸化窒素の影響

    村田綾,青木雄哉,氏原麻衣,吉岡 泰

    第56回日本植物生理学会年会 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京農業大学(東京都)   Country:Japan  

  20. シロイヌナズナ CRUMPLED LEAF タンハク質と相互作用するタンハク質の同定

    村田綾,浅野智哉,竹内公香,中村善紀,町田泰則,吉岡泰

    第54回日本植物生理学会年日本植物生理学会年会 

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    Event date: 2013.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:岡山大学   Country:Japan  

  21. シロイヌナズナ CRUMPLED LEAF タンハク質のオルカネラ局 在に必要な領域の解析

    竹内公香,北辻彩夏,角田亜希子,町田泰則,吉岡泰

    第54回日本植物生理学会年日本植物生理学会年会 

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    Event date: 2013.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山大学   Country:Japan  

  22. シロイヌナズナ CRL タンパク質のオルガネラ局在解析

    吉岡泰,北辻彩夏,角田亜希子,町田泰則

    第53回日本植物生理学会年日本植物生理学会年会 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:京都産業大学   Country:Japan  

  23. ヒメツリガネゴケ CRL は葉緑体分裂の完了に機能する

    杉田千恵子,加藤大和,吉岡泰,町田泰則,杉田護

    第53回日本植物生理学会年日本植物生理学会年会 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:京都産業大学   Country:Japan  

  24. シロイヌナズナ CRL タンパク質の色素体包膜局在に必要な領域の解析

    吉岡 泰、北辻 彩夏、角田 亜希子、町田 泰則

    日本植物学会第75回大会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学駒場キャンパス   Country:Japan  

  25. 体細胞の染色体が倍化するシロイヌナズナgigas cell1変異体の解析

    岩田 恵里子、 池田 早希、松永 幸大、吉岡 泰、伊藤 正樹

    日本植物学会第75回大会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:要旨集   Country:Japan  

  26. シロイヌナズナCRLタンパク質細胞内局在の解析

    吉岡 泰、角田 亜希子、町田 泰則

    第52回日本植物生理学会年会 

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    Event date: 2011.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:要旨集   Country:Japan  

  27. ヒメツリガネゴケCRUMPLED LEAF相同遺伝子の機能解析

    杉田 千恵子、加藤 大和、鶴見 尚子、吉岡 泰、町田 泰則、杉田 護

    第52回日本植物生理学会年会 

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    Event date: 2011.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:要旨集   Country:Japan  

  28. 体細胞の染色体が倍化するシロイヌナズナgigas cell1変異体の解析

    岩田 恵里子、松永 幸大、吉岡 泰、伊藤 正樹

    第52回日本植物生理学会年会 

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    Event date: 2011.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:要旨集   Country:Japan  

  29. ヒメツリガネゴケCRUMPLED LEAF相同遺伝子破壊株の解析

    植物学会第74回大会 

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    Event date: 2010.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  30. An analysis of CRUMPLED LEAF homologous genes of Physcomitrella patens by gene disruption International conference

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    Event date: 2010.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

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Research Project for Joint Research, Competitive Funding, etc. 2

  1. CRISPR-Cas9ゲノム編集技術を基盤としたラン科植物育種方法の確立 International coauthorship

    2017.4 - 2020.3

    二国間交流事業 共同研究 

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\7187080 ( Direct Cost: \7187080 )

  2. 色素体分裂制御遺伝子群ネットワークの内的・外的環境に対する応答の解析

    2006.4 - 2008.3

KAKENHI (Grants-in-Aid for Scientific Research) 5

  1. ゲノム編集技術を基盤としたコチョウラン育種方法の確立

    Grant number:22K05629  2022.4 - 2026.3

    科学研究費助成事業  基盤研究(C)

    吉岡 泰, 松本 省吾

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    Authorship:Principal investigator 

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    本研究は外来遺伝子のランゲノムへの組込みを伴わないゲノム編集技術の開発とそれを利用したランの育種を目指す。コチョウラン原種を材料として用い、低温プラズマ照射等を用いたラン細胞へのタンパク質核酸直接導入法、植物細胞内で遺伝子を一過的に高発現可能なベクターシステムを利用する。さらに、花や葉の形態、色などを司る遺伝子を標的としたゲノム編集を行い、新たな形質をもつコチョウランの育種を試みる。

  2. CRISPR-Cas9ゲノム編集技術を基盤としたラン科植物育種方法の確立

    2017.4 - 2020.3

    科学研究費補助金  二国間交流事業共同研究

    吉岡 泰

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    Authorship:Principal investigator 

  3. 細胞増殖分化に関与する色素体機能の解明

    2014.4 - 2017.3

    科学研究費補助金  基盤研究(C)

    吉岡 泰

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    Authorship:Principal investigator 

  4. 色素体分裂制御遺伝子群ネットワークの 内的・外的環境に対する応答の解析

    2005.4 - 2008.3

    科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

  5. 葉緑体および植物細胞の分裂に関与するシロイヌナズナ遺伝子の機能解析

    2002.4 - 2005.4

    科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

 

Teaching Experience (On-campus) 6

  1. 形態統御学講究3

    2022

  2. First Year Seminar B

    2017

  3. First Year Seminar B

    2016

  4. First Year Seminar B

    2015

  5. First Year Seminar A

    2012

  6. First Year Seminar A

    2011

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