Updated on 2021/04/06

写真a

 
SUZUKI Takayuki
 
Organization
Graduate School of Bioagricultural Sciences Avian Bioscience Research Center Associate professor
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences Department of Bioresource Sciences
Title
Associate professor

Degree 3

  1. 博士(バイオサイエンス) ( 2004.3   奈良先端科学技術大学院大学 ) 

  2. 修士(理工学) ( 2001.3   慶應義塾大学 ) 

  3. 学士(応用化学) ( 1999.3   慶應義塾大学 ) 

Research Interests 2

  1. 発生生物学

  2. 進化発生学

Research Areas 4

  1. Life Science / Genetics  / 遺伝学

  2. Life Science / Evolutionary biology  / 進化発生学

  3. Life Science / Cell biology  / 生化学

  4. Life Science / Developmental biology  / 発生生物学

Current Research Project and SDGs 1

  1. 骨格パターンの形成メカニズム

Research History 9

  1. Nagoya University   Graduate School of Bioagricultural Sciences Avian Bioscience Research Center   Associate professor

    2018.4

  2. Nagoya University   Graduate School of Science Division of Biological Science Cell Regulation   Lecturer

    2016.4 - 2018.3

  3. Nagoya University   Graduate School of Science Division of Biological Science   Assistant Professor

    2010.10 - 2016.3

  4. 独立行政法人科学技術振興機構 さきがけ研究者

    2008.10 - 2012.3

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    Country:Japan

  5. 東北大学加齢医学研究所 助教

    2007.4 - 2010.9

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    Country:Japan

  6. 日本学術振興会海外特別研究員

    2005.4 - 2007.3

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    Country:Japan

  7. 米国ウィスコンシン大学解剖学分野ポスドク

    2004.6 - 2007.3

  8. 日本学術振興会特別研究員(PD)

    2004.4 - 2005.3

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    Country:Japan

  9. 日本学術振興会特別研究員(DC2)

    2003.4 - 2004.3

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    Country:Japan

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Education 3

  1. Nara Institute of Science and Technology   Graduate School, Division of Biological Science

    - 2004.3

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    Country: Japan

  2. Keio University   Graduate School, Division of Science and Engineering

    - 2001.3

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    Country: Japan

  3. Keio University   Faculty of Science and Engineering

    - 1999.3

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    Country: Japan

Professional Memberships 2

  1. 発生生物学会

  2. 国際ニワトリ学術集会

Awards 1

  1. 日本遺伝学会奨励賞

    2019.9   日本遺伝学会  

    鈴木 孝幸

 

Papers 44

  1. The dynamic spatial and temporal relationships between the phalanx‐forming region and the interdigits determine digit identity in the chick limb autopod Invited Reviewed International journal

    Takayuki Suzuki, John F. Fallon

    Developmental Dynamics     2021.3

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    Authorship:Lead author, Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/dvdy.323

    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/dvdy.323

  2. Thalidomide and its metabolite 5‐hydroxythalidomide induce teratogenicity via the cereblon neosubstrate PLZF Reviewed International journal

    Satoshi Yamanaka, Hidetaka Murai, Daisuke Saito, Gembu Abe, Etsuko Tokunaga, Takahiro Iwasaki, Hirotaka Takahashi, Hiroyuki Takeda, Takayuki Suzuki, Norio Shibata, Koji Tamura, Tatsuya Sawasaki

    The EMBO Journal   Vol. 40 ( 4 )   2021.2

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:EMBO  

    DOI: 10.15252/embj.2020105375

    Web of Science

    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.15252/embj.2020105375

  3. Origin and evolutionary history of domestic chickens inferred from a large population study of Thai red junglefowl and indigenous chickens Invited Reviewed International journal

    Ayano Hata, Mitsuo Nunome, Thanathip Suwanasopee, Prateep Duengkae, Soontorn Chaiwatana, Wiyada Chamchumroon, Takayuki Suzuki, Skorn Koonawootrittriron, Yoichi Matsuda, Kornsorn Srikulnath

    Scientific Reports   Vol. 11 ( 1 )   2021.1

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title>In this study, we aimed to elucidate the origin of domestic chickens and their evolutionary history over the course of their domestication. We conducted a large-scale genetic study using mitochondrial DNA D-loop sequences and 28 microsatellite DNA markers to investigate the diversity of 298 wild progenitor red junglefowl (<italic>Gallus gallus</italic>) across two subspecies (<italic>G. g. gallus</italic> and <italic>G. g. spadiceus</italic>) from 12 populations and 138 chickens from 10 chicken breeds indigenous to Thailand. Twenty-nine D-loop sequence haplotypes were newly identified: 14 and 17 for Thai indigenous chickens and red junglefowl, respectively. Bayesian clustering analysis with microsatellite markers also revealed high genetic diversity in the red junglefowl populations. These results suggest that the ancestral populations of Thai indigenous chickens were large, and that a part of the red junglefowl population gene pool was not involved in the domestication process. In addition, some haplogroups that are distributed in other countries of Southeast Asia were not observed in either the red junglefowls or the indigenous chickens examined in the present study, suggesting that chicken domestication occurred independently across multiple regions in Southeast Asia.

    DOI: 10.1038/s41598-021-81589-7

    Other Link: http://www.nature.com/articles/s41598-021-81589-7

  4. DNA methylation analysis of multiple imprinted DMRs in Sotos syndrome reveals IGF2-DMR0 as a DNA methylation-dependent, P0 promoter-specific enhancer

    Watanabe H., Higashimoto K., Miyake N., Morita S., Horii T., Kimura M., Suzuki T., Maeda T., Hidaka H., Aoki S., Yatsuki H., Okamoto N., Uemura T., Hatada I., Matsumoto N., Soejima H.

    EUROPEAN JOURNAL OF HUMAN GENETICS   Vol. 28 ( SUPPL 1 ) page: 759 - 760   2020.12

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  5. The formation of multiple pituitary pouches from the oral ectoderm causes ectopic lens development in hedgehog signaling‐defective avian embryos Invited Reviewed International journal

    Yuki Taira, Yuya Ikuta, Sachiko Inamori, Mitsuo Nunome, Mikiharu Nakano, Takayuki Suzuki, Yoichi Matsuda, Masaoki Tsudzuki, Machiko Teramoto, Hideaki Iida, Hisato Kondoh

    Developmental Dynamics   Vol. 249 ( 12 ) page: 1425 - 1439   2020.12

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/dvdy.222

    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/dvdy.222

  6. Geographic Origin and Genetic Characteristics of Japanese Indigenous Chickens Inferred from Mitochondrial D-Loop Region and Microsatellite DNA Markers Invited Reviewed International journal

    Ayano Hata, Atsushi Takenouchi, Keiji Kinoshita, Momomi Hirokawa, Takeshi Igawa, Mitsuo Nunome, Takayuki Suzuki, Masaoki Tsudzuki

    Animals   Vol. 10 ( 11 ) page: 2074 - 2074   2020.11

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    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Japanese indigenous chickens have a long breeding history, possibly beginning 2000 years ago. Genetic characterization of Japanese indigenous chickens has been performed using mitochondrial D-loop region and microsatellite DNA markers. Their phylogenetic relationships with chickens worldwide and genetic variation within breeds have not yet been examined. In this study, the genetic characteristics of 38 Japanese indigenous chicken breeds were assessed by phylogenetic analyses of mitochondrial D-loop sequences compared with those of indigenous chicken breeds overseas. To evaluate the genetic relationships among Japanese indigenous chicken breeds, a STRUCTURE analysis was conducted using 27 microsatellite DNA markers. D-loop sequences of Japanese indigenous chickens were classified into five major haplogroups, A–E, among 15 haplogroups found in chickens worldwide. The haplogroup composition suggested that Japanese indigenous chickens originated mainly from China, with some originating from Southeast Asia. The STRUCTURE analyses revealed that Japanese indigenous chickens are genetically differentiated from chickens overseas; Japanese indigenous chicken breeds possess distinctive genetic characteristics, and Jidori breeds, which have been reared in various regions of Japan for a long time, are genetically close to each other. These results provide new insights into the history of chickens around Asia in addition to novel genetic data for the conservation of Japanese indigenous chickens.

    DOI: 10.3390/ani10112074

  7. Transcriptome analysis revealed misregulated gene expression in blastoderms of interspecific chicken and Japanese quail F1 hybrids Invited Reviewed International journal

    Satoshi Ishishita, Shoji Tatsumoto, Keiji Kinoshita, Mitsuo Nunome, Takayuki Suzuki, Yasuhiro Go, Yoichi Matsuda

    PLOS ONE   Vol. 15 ( 10 ) page: e0240183 - e0240183   2020.10

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Public Library of Science (PLoS)  

    DOI: 10.1371/journal.pone.0240183

  8. Primordial germ cell‐specific expression of eGFP in transgenic chickens Invited Reviewed International journal

    Yota Hagihara, Yuya Okuzaki, Kazuma Matsubayashi, Hidenori Kaneoka, Takayuki Suzuki, Shinji Iijima, Ken‐ichi Nishijima

    genesis   Vol. 58 ( 8 )   2020.8

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/dvg.23388

    Web of Science

    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/dvg.23388

  9. Enhancer functions underlying morphological diversity

    Suzuki Takayuki, Koshikawa Shigeyuki

    DEVELOPMENT GROWTH & DIFFERENTIATION   Vol. 62 ( 5 ) page: 263 - 264   2020.6

  10. How do signaling and transcription factors regulate both axis elongation and Hox gene expression along the anteroposterior axis? Invited Reviewed International journal

    Seiji Saito, Takayuki Suzuki

    Development, Growth & Differentiation   Vol. 62 ( 5 ) page: 363 - 375   2020.6

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    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/dgd.12682

    Web of Science

    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/dgd.12682

  11. Combined deletions of IHH and NHEJ1 cause chondrodystrophy and embryonic lethality in the Creeper chicken Invited Reviewed International journal

    Keiji Kinoshita, Takayuki Suzuki, Manabu Koike, Chizuko Nishida, Aki Koike, Mitsuo Nunome, Takeo Uemura, Kenji Ichiyanagi, Yoichi Matsuda

    Communications Biology   Vol. 3 ( 1 )   2020.3

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title>The Creeper (<italic>Cp</italic>) chicken is characterized by chondrodystrophy in <italic>Cp</italic>/+ heterozygotes and embryonic lethality in <italic>Cp</italic>/<italic>Cp</italic> homozygotes. However, the genes underlying the phenotypes have not been fully known. Here, we show that a 25 kb deletion on chromosome 7, which contains the Indian hedgehog (<italic>IHH</italic>) and non-homologous end-joining factor 1 (<italic>NHEJ1</italic>) genes, is responsible for the <italic>Cp</italic> trait in Japanese bantam chickens. <italic>IHH</italic> is essential for chondrocyte maturation and is downregulated in the <italic>Cp/+</italic> embryos and completely lost in the <italic>Cp</italic>/<italic>Cp</italic> embryos. This indicates that chondrodystrophy is caused by the loss of <italic>IHH</italic> and that chondrocyte maturation is delayed in <italic>Cp</italic>/<italic>+</italic> heterozygotes. The <italic>Cp</italic>/<italic>Cp</italic> homozygotes exhibit impaired DNA double-strand break (DSB) repair due to the loss of <italic>NHEJ1</italic>, resulting in DSB accumulation in the vascular and nervous systems, which leads to apoptosis and early embryonic death.

    DOI: 10.1038/s42003-020-0870-z

    Web of Science

    Other Link: http://www.nature.com/articles/s42003-020-0870-z

  12. DNA methylation analysis of multiple imprinted DMRs in Sotos syndrome reveals IGF2 ‐DMR0 as a DNA methylation‐dependent, P0 promoter‐specific enhancer Invited Reviewed International journal

    Hidetaka Watanabe, Ken Higashimoto, Noriko Miyake, Sumiyo Morita, Takuro Horii, Mika Kimura, Takayuki Suzuki, Toshiyuki Maeda, Hidenori Hidaka, Saori Aoki, Hitomi Yatsuki, Nobuhiko Okamoto, Tetsuji Uemura, Izuho Hatada, Naomichi Matsumoto, Hidenobu Soejima

    The FASEB Journal   Vol. 34 ( 1 ) page: 960 - 973   2020.1

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1096/fj.201901757r

    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1096/fj.201901757R

  13. PRDM14 and BLIMP1 control the development of chicken primordial germ cells

    Okuzaki Yuya, Kaneoka Hidenori, Suzuki Takayuki, Hagihara Yota, Nakayama Yuki, Murakami Seitaro, Murase Yusuke, Kuroiwa Atsushi, Iijima Shinji, Nishijima Ken-ichi

    DEVELOPMENTAL BIOLOGY   Vol. 455 ( 1 ) page: 32 - 41   2019.11

  14. Identification of transgene integration site and anatomical properties of fluorescence intensity in a EGFP transgenic chicken line. Reviewed International journal

    Tsujino K, Okuzaki Y, Hibino N, Kawamura K, Ozaki Y, Ishishita S, Kuroiwa A, Iijima S, Matsuda Y, Nishijima K, Suzuki T

    Development, growth & differentiation     2019.10

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  15. Recommendation of Brilliant Blue instead of Fast Green as a dye at in ovo electroporation. Reviewed International journal

    Saito S, Kawamura K, Matsuda Y, Suzuki T

    Development, growth & differentiation     2019.10

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    Language:English   Publishing type:Research paper (scientific journal)  

  16. Brilliant Blue as an alternative dye to Fast Green for in ovo electroporation

    Saito Seiji, Kawamura Kazuki, Matsuda Yoichi, Suzuki Takayuki

    DEVELOPMENT GROWTH & DIFFERENTIATION   Vol. 61 ( 7-8 ) page: 402 - 409   2019.9

  17. Identification of transgene integration site and anatomical properties of fluorescence intensity in a EGFP transgenic chicken line

    Tsujino Kaori, Okuzaki Yuya, Hibino Nobuyuki, Kawamura Kazuki, Saito Seiji, Ozaki Yumi, Ishishita Satoshi, Kuroiwa Atsushi, Iijima Shinji, Matsuda Yoichi, Nishijima Kenichi, Suzuki Takayuki

    DEVELOPMENT GROWTH & DIFFERENTIATION   Vol. 61 ( 7-8 ) page: 393 - 401   2019.9

  18. Self-organized formation of developing appendages from murine pluripotent stem cells

    Mori Shunsuke, Sakakura Eriko, Tsunekawa Yuji, Hagiwara Masaya, Suzuki Takayuki, Eiraku Mototsugu

    NATURE COMMUNICATIONS   Vol. 10   2019.8

  19. PRDM14 and BLIMP1 control the development of chicken primordial germ cells. Reviewed International journal

    Okuzaki Y, Kaneoka H, Suzuki T, Hagihara Y, Nakayama Y, Murakami S, Murase Y, Kuroiwa A, Iijima S, Nishijima KI

    Developmental biology   Vol. 455 ( 1 ) page: 32 - 41   2019.7

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  20. Anatomical integration of the sacral-hindlimb unit coordinated by GDF11 underlies variation in hindlimb positioning in tetrapods. Reviewed

    Matsubara Y., Hirasawa T., Egawa S., Hattori A., Suganuma T., Kohara Y., Nagai T., Tamura K., Kuratani S., Kuroiwa A., Suzuki T.

    Nature Ecol. Evol.   Vol. 1   page: 1392-1399   2017.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  21. Anatomical integration of the sacral-hindlimb unit coordinated by GDF11 underlies variation in hindlimb positioning in tetrapods Reviewed International journal

    Yoshiyuki Matsubara, Tatsuya Hirasawa, Shiro Egawa, Ayumi Hattori, Takaya Suganuma, Yuhei Kohara, Tatsuya Nagai, Koji Tamura, Shigeru Kuratani, Atsushi Kuroiwa, Takayuki Suzuki

    Nature Ecology and Evolution   Vol. 1 ( 9 ) page: 1392 - 1399   2017.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Nature Publishing Group  

    Elucidating how body parts from different primordia are integrated during development is essential for understanding the nature of morphological evolution. In tetrapod evolution, while the position of the hindlimb has diversified along with the vertebral formula, the mechanism responsible for this coordination has not been well understood. However, this synchronization suggests the presence of an evolutionarily conserved developmental mechanism that coordinates the positioning of the hindlimb skeleton derived from the lateral plate mesoderm with that of the sacral vertebrae derived from the somites. Here we show that GDF11 secreted from the posterior axial mesoderm is a key factor in the integration of sacral vertebrae and hindlimb positioning by inducing Hox gene expression in two different primordia. Manipulating the onset of GDF11 activity altered the position of the hindlimb in chicken embryos, indicating that the onset of Gdf11 expression is responsible for the coordinated positioning of the sacral vertebrae and hindlimbs. Through comparative analysis with other vertebrate embryos, we also show that each tetrapod species has a unique onset timing of Gdf11 expression, which is tightly correlated with the anteroposterior levels of the hindlimb bud. We conclude that the evolutionary diversity of hindlimb positioning resulted from heterochronic shifts in Gdf11 expression, which led to coordinated shifts in the sacral-hindlimb unit along the anteroposterior axis.

    DOI: 10.1038/s41559-017-0247-y

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  22. A quantitative approach to understanding vertebrate limb morphogenesis at the macroscopic tissue level Reviewed International journal

    Takayuki Suzuki, Yoshihiro Morishita

    CURRENT OPINION IN GENETICS & DEVELOPMENT   Vol. 45   page: 108 - 114   2017.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:CURRENT BIOLOGY LTD  

    To understand organ morphogenetic mechanisms, it is essential to clarify how spatiotemporally-regulated molecular/cellular dynamics causes physical tissue deformation. In the case of vertebrate limb development, while some of the genes and oriented cell behaviors underlying morphogenesis have been revealed, tissue deformation dynamics remains incompletely understood. We here introduce our recent work on the reconstruction of tissue deformation dynamics in chick limb development from cell lineage tracing data. This analysis has revealed globally-aligned anisotropic tissue deformation along the proximo-distal axis not only in the distal region but also in the whole limb bud. This result points to a need, as a future challenge, to find oriented molecular/cellular behaviors for realizing the observed anisotropic tissue deformation in both proximal and distal regions, which will lead to systems understanding of limb morphogenesis.

    DOI: 10.1016/j.gde.2017.04.005

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  23. Quantitative and comparative analysis of tissue deformation dynamics for chick and Xenopus limb morphogenesis Reviewed International journal

    Yoshihiro Morishita, Takayuki Suzuki, Hitoshi Yokoyama, Yasuhiro Kamei, Koji Tamura, Aiko Kawasumi

    MECHANISMS OF DEVELOPMENT   Vol. 145   page: S40 - S40   2017.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    DOI: 10.1016/j.mod.2017.04.060

    Web of Science

  24. Upstream regulation for initiation of restricted Shh expression in the chick limb bud Reviewed International journal

    Haruka Matsubara, Daisuke Saito, Gembu Abe, Hitoshi Yokoyama, Takayuki Suzuki, Koji Tamura

    DEVELOPMENTAL DYNAMICS   Vol. 246 ( 5 ) page: 417 - 430   2017.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    Background: The organizing center, which serves as a morphogen source, has crucial functions in morphogenesis in animal development. The center is necessarily located in a certain restricted area in the morphogenetic field, and there are several ways in which an organizing center can be restricted. The organizing center for limb morphogenesis, the ZPA (zone of polarizing activity), specifically expresses the Shh gene and is restricted to the posterior region of the developing limb bud.Results: The pre-pattern along the limb anteroposterior axis, provided by anterior Gli3 expression and posterior Hand2 expression, seems insufficient for the initiation of Shh expression restricted to a narrow, small spot in the posterior limb field. Comparison of the spatiotemporal patterns of gene expression between Shh and some candidate genes (Fgf8, Hoxd10, Hoxd11, Tbx2, and Alx4) upstream of Shh expression suggested that a combination of these genes' expression provides the restricted initiation of Shh expression.Conclusions: Taken together with results of functional assays, we propose a model in which positive and negative transcriptional regulatory networks accumulate their functions in the intersection area of their expression regions to provide a restricted spot for the ZPA, the source of morphogen, Shh. Developmental Dynamics 246:417-430, 2017. (c) 2017 Wiley Periodicals, Inc.

    DOI: 10.1002/dvdy.24493

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  25. Transgene introduction into the chick limb bud by electroporation Reviewed International journal

    Shogo Ueda, Takayuki Suzuki, Mikiko Tanaka

    Methods in Molecular Biology   Vol. 1650   page: 203 - 208   2017

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    Language:English   Publishing type:Part of collection (book)   Publisher:Humana Press Inc.  

    Electroporation enables delivering bionanomolecules, such as DNAs, RNAs, siRNAs, and morpholinos, into chick embryos in a spatially and temporally restricted fashion. Recent advances in electroporation techniques allowed us to deliver transgenes into the restricted area of the limb bud and to analyze the function of the enhancers in the limb field. Here, we describe the introduction of transgenes by electroporation in the limb field and its application on enhancer analysis.

    DOI: 10.1007/978-1-4939-7216-6_13

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  26. Efficient harvesting methods for early-stage snake and turtle embryos Reviewed International journal

    Yoshiyuki Matsubara, Atsushi Kuroiwa, Takayuki Suzuki

    DEVELOPMENT GROWTH & DIFFERENTIATION   Vol. 58 ( 3 ) page: 241 - 249   2016.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    Reptile development is an intriguing research target for understating the unique morphogenesis of reptiles as well as the evolution of vertebrates. However, there are numerous difficulties associated with studying development in reptiles. The number of available reptile eggs is usually quite limited. In addition, the reptile embryo is tightly adhered to the eggshell, making it a challenge to isolate reptile embryos intact. Furthermore, there have been few reports describing efficient procedures for isolating intact embryos especially prior to pharyngula stage. Thus, the aim of this review is to present efficient procedures for obtaining early-stage reptilian embryos intact. We first describe the method for isolating early-stage embryos of the Japanese striped snake. This is the first detailed method for obtaining embryos prior to oviposition in oviparous snake species. Second, we describe an efficient strategy for isolating early-stage embryos of the soft-shelled turtle.

    DOI: 10.1111/dgd.12278

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  27. Inactivation of Sonic Hedgehog Signaling and Polydactyly in Limbs of Hereditary Multiple Malformation, a Novel Type of Talpid Mutant. Reviewed International journal

    Yoshiyuki Matsubara, Mikiharu Nakano, Kazuki Kawamura, Masaoki Tsudzuki, Jun-Ichi Funahashi, Kiyokazu Agata, Yoichi Matsuda, Atsushi Kuroiwa, Takayuki Suzuki

    Frontiers in cell and developmental biology   Vol. 4   page: 149 - 149   2016

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    Hereditary Multiple Malformation (HMM) is a naturally occurring, autosomal recessive, homozygous lethal mutation found in Japanese quail. Homozygote embryos (hmm-/-) show polydactyly similar to talpid2 and talpid3 mutants. Here we characterize the molecular profile of the hmm-/- limb bud and identify the cellular mechanisms that cause its polydactyly. The hmm-/- limb bud shows a severe lack of sonic hedgehog (SHH) signaling, and the autopod has 4 to 11 unidentifiable digits with syn-, poly-, and brachydactyly. The Zone of Polarizing Activity (ZPA) of the hmm-/- limb bud does not show polarizing activity regardless of the presence of SHH protein, indicating that either the secretion pathway of SHH is defective or the SHH protein is dysfunctional. Furthermore, mesenchymal cells in the hmm-/- limb bud do not respond to ZPA transplanted from the normal limb bud, suggesting that signal transduction downstream of SHH is also defective. Since primary cilia are present in the hmm-/- limb bud, the causal gene must be different from talpid2 and talpid3. In the hmm-/- limb bud, a high amount of GLI3A protein is expressed and GLI3 protein is localized to the nucleus. Our results suggest that the regulatory mechanism of GLI3 is disorganized in the hmm-/- limb bud.

    DOI: 10.3389/fcell.2016.00149

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  28. AP-2 beta is a transcriptional regulator for determination of digit length in tetrapods Reviewed International journal

    Ryohei Seki, Keiichi Kitajima, Haruka Matsubara, Takayuki Suzuki, Daisuke Saito, Hitoshi Yokoyama, Koji Tamura

    DEVELOPMENTAL BIOLOGY   Vol. 407 ( 1 ) page: 75 - 89   2015.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The species-specific morphology of digits in the tetrapod limb, including the length and number of metacarpal, metatarsal, and phalangeal bones, suggests that a common developmental mechanism for digit formation is modified in a species-specific manner. Here, we examined the function of the AP-2 beta transcription factor in regulating digit length in the chicken autopod. Mutations in the gene encoding AP-2 beta are are associated with Char syndrome, a human autosomal dominant disorder. Char syndrome patients exhibit autopod skeletal defects, including loss of phalanges and shortened fingers, suggestive of a function for AP-2 beta in normal digit development. The ectopic expression of two different dominant-negative forms of chick AP-2 beta, equivalent to mutant forms associated with human Char syndrome, in the developing chick hindlimb bud resulted in defective digit formation, including reductions in the number and length of phalanges and metatarsals. A detailed analysis of the AP-2 beta expression pattern in the limb bud indicated a correlation between the pattern/duration of AP-2 beta expression in the limb mesenchyme and digit length in three amniote species, the chicken, mouse and gecko. In addition, we found that AP-2 beta expression expression was downstream of Fgf signals from the apical ectodermal ridge, which is crucial in digit morphogenesis, and that excessive AP-2 beta function resulted in dysregulated digit length. Taken together, these results suggest that AP-2 beta functions as a novel transcriptional regulator for digit morphogenesis. (C) 2015 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.ydbio.2015.08.006

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  29. Quantitative analysis of tissue deformation dynamics reveals three characteristic growth modes and globally aligned anisotropic tissue deformation during chick limb development. Reviewed

    Morishita Y, Kuroiwa A, Suzuki T.

    Development   Vol. 142 ( 9 ) page: 1672-1683   2015.5

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242

  30. An efficient embryonic culture method for the Japanese striped snake, Elaphe quadrivirgata, and its early developmental stages. Reviewed

    Matsubara Y., Sakai A., Kuroiwa A., Suzuki T.

    Dev. Growth Differ.   Vol. 56   page: 573-582   2014.10

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  31. Efficient embryonic culture method for the Japanese striped snake, Elaphe quadrivirgata, and its early developmental stages Reviewed International journal

    Yoshiyuki Matsubara, Atsushi Sakai, Atsushi Kuroiwa, Takayuki Suzuki

    DEVELOPMENT GROWTH & DIFFERENTIATION   Vol. 56 ( 8 ) page: 573 - 582   2014.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    The morphogenesis of snake embryos is an elusive yet fascinating research target for developmental biologists. However, few data exist on development of early snake embryo due to limited availability of pregnant snakes, and the need to harvest early stage embryos directly from pregnant snakes before oviposition without knowing the date of fertilization. We established an ex vivo culture method for early snake embryos using the Japanese striped snake, Elaphe quadrivirgata. This method, which we named sausage-style (SS) culture, allows us to harvest snake embryos at specific stages for each experiment. Using this SS culture system, we calculated somite formation rate at early stages before oviposition. The average somite formation rate between 6/7 and 12/13 somite stages was 145.9min, between 60/70 and 80/91 somite stages 42.4min, and between 113-115 and 126/127 somite stages 71min. Thus, somite formation rate that we observed during early snake embryogenesis was changed over time. We also describe a developmental staging series for E.quadrivirgata. This is the first report of a developmental series of early snake embryogenesis prior to oviposition by full-color images with high-resolution. We propose that the SS culture system is an easy method for treating early snake embryos ex vivo.

    DOI: 10.1111/dgd.12157

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  32. Bayesian inference of whole-organ deformation dynamics from limited space-time point data Reviewed International journal

    Yoshihiro Morishita, Takayuki Suzuki

    JOURNAL OF THEORETICAL BIOLOGY   Vol. 357   page: 74 - 85   2014.9

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    To understand the morphogenetic mechanisms of organ development and regeneration, it is essential to clarify the inter-hierarchical relationship between microscopic, molecular/cellular activities and organ-level tissue deformation dynamics. While the former have been studied for several decades, the latter macroscopic geometrical information about physical tissue deformation - is often missing, especially for many vertebrates. This is mainly because live recording of detailed cell behaviors in whole tissues during vertebrate organogenesis is technically difficult. In this study, we have developed a novel method that combines snapshot lineage tracing with Bayesian statistical estimation to construct whole-organ deformation maps from landmark data on limited numbers of space-time points. Following the validation of the method using artificially generated data sets, we applied it to the analysis of tissue deformation dynamics in chick limb development. A quantitative tissue deformation map for St.23-St.24 has been constructed, and its precision has been proven by evaluating its predictive performance. Geometrical analyses of the map have revealed a spatially heterogeneous volume growth pattern that is consistent with the expression pattern of a major morphogen and anisotropic tissue deformation along an axis. Thus, our method enables deformation dynamics analysis in organogenesis using practical lineage marking techniques. (C) 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license

    DOI: 10.1016/j.jtbi.2014.04.027

    Web of Science

  33. Reconstruction and in vivo analysis of the extinct tbx5 gene from ancient wingless moa (Aves: Dinornithiformes) Reviewed International journal

    Leon Huynen, Takayuki Suzuki, Toshihiko Ogura, Yusuke Watanabe, Craig D. Millar, Michael Hofreiter, Craig Smith, Sara Mirmoeini, David M. Lambert

    BMC EVOLUTIONARY BIOLOGY   Vol. 14   page: 75   2014.5

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    Background: The forelimb-specific gene tbx5 is highly conserved and essential for the development of forelimbs in zebrafish, mice, and humans. Amongst birds, a single order, Dinornithiformes, comprising the extinct wingless moa of New Zealand, are unique in having no skeletal evidence of forelimb-like structures.
    Results: To determine the sequence of tbx5 in moa, we used a range of PCR-based techniques on ancient DNA to retrieve all nine tbx5 exons and splice sites from the giant moa, Dinornis. Moa Tbx5 is identical to chicken Tbx5 in being able to activate the downstream promotors of fgf10 and ANF. In addition we show that missexpression of moa tbx5 in the hindlimb of chicken embryos results in the formation of forelimb features, suggesting that Tbx5 was fully functional in wingless moa. An alternatively spliced exon 1 for tbx5 that is expressed specifically in the forelimb region was shown to be almost identical between moa and ostrich, suggesting that, as well as being fully functional, tbx5 is likely to have been expressed normally in moa since divergence from their flighted ancestors, approximately 60 mya.
    Conclusions: The results suggests that, as in mice, moa tbx5 is necessary for the induction of forelimbs, but is not sufficient for their outgrowth. Moa Tbx5 may have played an important role in the development of moa's remnant forelimb girdle, and may be required for the formation of this structure. Our results further show that genetic changes affecting genes other than tbx5 must be responsible for the complete loss of forelimbs in moa.

    DOI: 10.1186/1471-2148-14-75

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  34. How is digit identity determined during limb development? Reviewed International journal

    Takayuki Suzuki

    DEVELOPMENT GROWTH & DIFFERENTIATION   Vol. 55 ( 1 ) page: 130 - 138   2013.1

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    Digit identity has been studied using the chick embryo as a model system for more than 40 years. Using this model system, several milestone findings have been reported, such as the apical ectodermal ridge (AER), the zone of polarizing activity (ZPA), the Shh gene, and the theory of morphogen and positional information. These experimental results and models provided context for understanding pattern formation in developmental biology. The focus of this review is on the determination of digit identity during limb development. First, the history of studies on digit identity determination is described, followed by descriptions of the molecular mechanisms and current models for determination of digit identity. Finally, future questions and remarkable points will be discussed.

    DOI: 10.1111/dgd.12022

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  35. Identification of Spontaneous Mutations Within the Long-Range Limb-Specific Sonic Hedgehog Enhancer (ZRS) That Alter Sonic Hedgehog Expression in the Chicken Limb Mutants oligozeugodactyly and Silkie Breed Reviewed International journal

    Sarah A. Maas, Takayuki Suzuki, John F. Fallon

    DEVELOPMENTAL DYNAMICS   Vol. 240 ( 5 ) page: 1212 - 1222   2011.5

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    The evolutionarily conserved, non-coding similar to 800-base-pair (bp) zone of polarizing activity (ZPA) regulatory sequence (ZRS) controls Shh expression in the posterior limb. We report that the chicken mutant oligozeugodactyly (ozd), which lacks limb Shh expression, has a large deletion within the ZRS. Furthermore, the preaxial polydactylous, Silkie Breed chicken, which develops ectopic anterior limb Shh expression, has a single bp change within the ZRS. Using an in vivo reporter assay to examine enhancer function in the chick limb, we demonstrate that the wild-type ZRS drives beta-galactosidase reporter expression in the ZPA of both wild-type and ozd limbs. The Silkie ZRS drives beta-galactosidase in both posterior and anterior Shh domains in wild-type limb buds. These results support the hypothesis that the ZRS integrates positive and negative prepatterned regulatory inputs in the chicken model system and demonstrate the utility of the chicken limb as an efficient genetic system for gene regulatory studies. Developmental Dynamics 240: 1212-1222, 2011. (C) 2011 Wiley-Liss, Inc.

    DOI: 10.1002/dvdy.22634

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  36. Identification of a Primary Target of Thalidomide Teratogenicity Reviewed International journal

    Takumi Ito, Hideki Ando, Takayuki Suzuki, Toshihiko Ogura, Kentaro Hotta, Yoshimasa Imamura, Yuki Yamaguchi, Hiroshi Handa

    SCIENCE   Vol. 327 ( 5971 ) page: 1345 - 1350   2010.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER ASSOC ADVANCEMENT SCIENCE  

    Half a century ago, thalidomide was widely prescribed to pregnant women as a sedative but was found to be teratogenic, causing multiple birth defects. Today, thalidomide is still used in the treatment of leprosy and multiple myeloma, although how it causes limb malformation and other developmental defects is unknown. Here, we identified cereblon (CRBN) as a thalidomide-binding protein. CRBN forms an E3 ubiquitin ligase complex with damaged DNA binding protein 1 (DDB1) and Cul4A that is important for limb outgrowth and expression of the fibroblast growth factor Fgf8 in zebrafish and chicks. Thalidomide initiates its teratogenic effects by binding to CRBN and inhibiting the associated ubiquitin ligase activity. This study reveals a basis for thalidomide teratogenicity and may contribute to the development of new thalidomide derivatives without teratogenic activity.

    DOI: 10.1126/science.1177319

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  37. Electroporation into the limb: Beyond misexpression Reviewed International journal

    Takayuki Suzuki, Toshihiko Ogura

    Electroporation and Sonoporation in Developmental Biology     page: 85 - 96   2009

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    Language:English   Publishing type:Part of collection (book)   Publisher:Springer Japan  

    Limb development has been studied for over 100 years by several generations of developmental biologists. The developing limb is one of the best models with which to study pattern formation in vertebrates. We have used chick limb development to answer a simple but basic question, namely, why heterogeneous tissues are formed at correct positions and times from a homogeneous population of cells (Pearse &amp
    Tabin, 1998). Limb development starts as two pairs of tissue bulges in the lateral plate meso-derm (LPM). These are called the forelimb and hindlimb fields (Fig. 9.1). After limb initiation, one can clearly identify three-dimensional axes in the limb buds: the proximal-distal (PD
    from shoulder to fingers), dorso-ventral (DV
    from back to palm), and antero-posterior (AP
    from thumb to little fingers) axes. Morphological changes and differences along these three axes are determined by pattern formation during limb bud stages. Following establishment of these axes, one can visually recognize condensation of cartilages. Muscles, tendons, and neurons migrate and differentiate after cartilage formation. Because the stages and events are easily recognized morphologically and in detail, it is therefore the limb bud is an excellent model with which to study the molecular mechanisms of embryonic patterning and tissue differentiation in vertebrates. © 2009 Springer Japan.

    DOI: 10.1007/978-4-431-09427-2_9

    Scopus

  38. Congenic method in the chick limb buds by electroporation Reviewed International journal

    Takayuki Suzuki, Toshihiko Ogura

    DEVELOPMENT GROWTH & DIFFERENTIATION   Vol. 50 ( 6 ) page: 459 - 465   2008.8

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    Electroporation is a powerful tool with which to study limb development. Limb development, however, remains an intricate series of events, requiring the precise dissection of developmental processes using relevant transgenes. In this review, we describe the anatomy of the limb field as the basis of targeted electroporation, and specific expression vectors are discussed. We share a useful protocol for electroporation of chick limb buds, and the expression pattern of enhanced green fluorescent protein in the limb buds is used to demonstrate relevant embryonic patterning. Finally, useful trouble-shooting techniques are described.

    DOI: 10.1111/j.1440-169x.2008.01054.x

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  39. Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity Reviewed International journal

    Takayuki Suzuki, Sean M. Hasso, John F. Fallon

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 105 ( 11 ) page: 4185 - 4190   2008.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATL ACAD SCIENCES  

    The zone of polarizing activity is the primary signaling center controlling anterior-posterior patterning of the amniote limb bud. The autopodial interdigits (IDs) are secondary signaling centers proposed to determine digit identity by acting on the cells of the digital ray. Here, we focus on events accompanying digital fate determination and define a region of the digital ray that expresses Sox9 and Bmpr1b and is phosphorylated-SMAD1/5/8 (p-SMAD1/5/8) positive. We name this region the phalanx-forming region (PFR), and show that the PFR cells arise from the distal subridge mesenchyme of digital ray. This phalanx-forming cell lineage is subsequently committed to the cartilage lineage; the fate of these cells is initially labile but becomes fixed as they are incorporated into the condensed cartilage of the digit primordium. Using an in vivo reporter assay, we establish that each digital PFR has a unique p-SMAD1/5/8 activity signature. In addition, we show that changes in this activity correlate with the identity of the digit that forms after experimental manipulation, supporting the idea that threshold signaling levels can lead to different developmental outcomes in a morphogenetic field. Our data define the molecular profile of the PFR, and we propose a model for understanding formation and variation of digits during autopodial development.

    DOI: 10.1073/pnas.0707899105

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  40. Chick Dach1 interacts with the Smad complex and Sin3a to control AER formation and limb development along the proximodistal axis Reviewed International journal

    Y Kida, Y Maeda, T Shiraishi, T Suzuki, T Ogura

    DEVELOPMENT   Vol. 131 ( 17 ) page: 4179 - 4187   2004.9

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    Based on recent data, a new view is emerging that vertebrate Dachshund (Dach) proteins are components of Six1/6 transcription factor-dependent signaling cascades. Although Drosophila data strongly suggest a tight link between Dpp signaling and the Dachshund gene, a functional relationship between vertebrate Dach and BMP signaling remains undemonstrated. We report that chick Dach1 interacts with the Smad complex and the corepressor mouse Sin3a, thereby acting as a repressor of BMP-mediated transcriptional control. In the limb, this antagonistic action regulates the formation of the apical ectodermal ridge (AER) in both the mesenchyme and the AER itself, and also controls pattern formation along the proximodistal axis of the limb. Our data introduce a new paradigm of BMP antagonism during limb development mediated by Dach1, which is now proven to function in different signaling cascades with distinct interacting partners.

    DOI: 10.1242/dev.01252

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  41. Tbx genes specify posterior digit identity through Shh and BMP signaling Reviewed International journal

    T Suzuki, J Takeuchi, K Koshiba-Takeuchi, T Ogura

    DEVELOPMENTAL CELL   Vol. 6 ( 1 ) page: 43 - 53   2004.1

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    Despite extensive studies on the anterior-posterior (AP) axis formation of limb buds, mechanisms that specify digit identities along the AP axis remain obscure. Using the four-digit chick leg as a model, we report here that Tbx2 and Tbx3 specify the digit identities of digits IV and III, respectively. Misexpression of Tbx2 and Tbx3 induced posterior homeotic transformation of digit III to digit IV and digit II to digit III, respectively. Conversely, misexpression of their mutants VP16DeltaTbx2 and VP16DeltaTbx3 induced anterior transformation. In both cases, alterations in the expression of several markers (e.g., BMP2, Shh, and HoxD genes) were observed. In addition, Tbx2 and Tbx3 rescued Noggin-mediated inhibition of interdigital BMP signaling, signaling which is pivotal in establishing digit identities. Hence, we conclude that Tbx3 specifies digit III, and the combination of Tbx2 and Tbx3 specifies digit IV, acting together with the interdigital BMP signaling cascade.

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    PubMed

  42. Tbx5 and Tbx4 trigger limb initiation through activation of the Wnt/Fgf signaling cascade Reviewed International journal

    JK Takeuchi, K Koshiba-Takeuchi, T Suzuki, M Kamimura, K Ogura, T Ogura

    DEVELOPMENT   Vol. 130 ( 12 ) page: 2729 - 2739   2003.6

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    A tight loop between members of the fibroblast growth factor and the Wnt families plays a key role in the initiation of vertebrate limb development. We show for the first time that Tbx5 and Tbx4 are directly involved in this process. When dominant-negative forms of these Tbx genes were misexpressed in the chick prospective limb fields, a limbless phenotype arose with repression of both Wnt and Fgf genes By contrast, when Tbx5 and Tbx4 were misexpressed in the flank, an additional wing-like and an additional leg-like limbs were induced, respectively. This additional limb formation was accompanied by the induction of both Wnt and Fgf genes These results highlight the pivotal roles of Tbx5 and Tbx4 during limb initiation, specification of forelimb/hindlimb and evolution of tetrapod limbs, placing Tbx genes at the center of a highly conserved genetic program.

    DOI: 10.1242/dev.00474

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  43. Potentiation of insulin-related signal transduction by a novel protein-tyrosine phosphatase inhibitor, Et-3,4-dephostatin, on cultured 3T3-L1 adipocytes Reviewed International journal

    T Suzuki, A Hiroki, T Watanabe, T Yamashita, Takei, I, K Umezawa

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 276 ( 29 ) page: 27511 - 27518   2001.7

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    We previously isolated dephostatin from Streptomyces as a novel inhibitor of CD45-associated protein-tyrosine phosphatase. We prepared Et-3,4-dephostatin as a stable analogue and found it to inhibit PTP-1B and SHPTP-1 protein-tyrosine phosphatases selectively but not to inhibit CD45 and leukocyte common antigen-related phosphatase ones effectively. Et-3,4-dephostatin increased the tyrosine phosphorylation of the insulin receptor and insulin receptor substrate-1 with or without insulin in differentiated 3T3-L1 mouse adipocytes. The increase of tyrosine phosphorylation by Et-3,4-dephostatin was more prominent in 6-h than in 30-min incubation. It also increased phosphorylation and activation of Akt with or without insulin. Et-3,4-dephostatin also enhanced translocation of glucose transporter 4 from the cytoplasm to the membrane and 2-deoxy-glucose transport. Et-3,4-dephostatin-induced glucose uptake was inhibited by SB203580, a p38 inhibitor, but not by PD98059, a MEK inhibitor, or by cycloheximide as insulin-induced uptake. Interestingly, although LY294002, a phosphatidylinositol 3-kinase inhibitor, inhibited the insulin-induced glucose uptake completely, it only partially inhibited the Et-3,4-dephostatin-induced uptake. It also blocked insulin-induced glucose transporter 4 translocation but not the Et-3,4-dephostatin-induced one. The increase in c-Cbl tyrosine phosphorylation caused by Et-3,4 dephostatin was stronger than that in insulin receptor phosphorylation, These observations indicate that a phosphatidylinositol 3-kinase-independent pathway involving c-Cbl is more important in Et-3,4-dephostatin-induced glucose uptake than in insulin-induced uptake, Et-3,4-dephostatin showed an in vivo antidiabetic effect in terms of reducing the high blood glucose level in KK-A(y) mice after oral administration. Thus, Et-3,4-dephostatin potentiated insulin-related signal transductions in cultured mouse adipocytes and showed an antidiabetic effect in mice.

    Web of Science

  44. Structure–Activity Relationship and Rational Design of 3,4-Dephostatin Derivatives as Protein Tyrosine Phosphatase Inhibitors Invited Reviewed International journal

    Takumi Watanabe, Takayuki Suzuki, Yoji Umezawa, Tomio Takeuchi, Masami Otsuka, Kazuo Umezawa

    Tetrahedron   Vol. 56 ( 5 ) page: 741 - 752   2000.1

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/s0040-4020(99)01058-3

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Books 2

  1. Secondary Smad1/5/8-dependent signaling downstream of SHH determines digit identity. International journal

    SUZUKI Takayuki( Role: Joint author ,  pp151-160)

    Springer  2014 

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    Language:Japanese Book type:Scholarly book

  2. Electroporation into the limb: beyond misexpression. International journal

    Suzuki T, Ogura T( Role: Joint author ,  pp85-96)

    Springer  2009 

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    Language:Japanese Book type:Scholarly book

MISC 10

  1. 後肢発生研究で見えてきた、胴の長さの進化 Invited International coauthorship International journal

    黒岩 厚, 鈴木 孝幸

    Natureダイジェスト   Vol. 15   2018

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

  2. マヘビ初期胚採取奮闘記 International coauthorship International journal

    松原由幸, 鈴木孝幸

    実験医学1月号     page: 102 - 106   2017.1

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  3. Molecular regulation of the formation of the Phalanx Forming Region (PFR) at autopod stages of limb development International coauthorship International journal

    Joseph J. Lancman, Takayuki Suzuki, Sean M. Hasso, Yina Li, Chin Chiang, John F. Fallon

    DEVELOPMENTAL BIOLOGY   Vol. 356 ( 1 ) page: 170 - 171   2011.8

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    DOI: 10.1016/j.ydbio.2011.05.633

    Web of Science

  4. サリドマイド催奇性の原因因子の発見 International coauthorship International journal

    伊藤拓水, 安藤秀樹, 鈴木孝幸, 小椋利彦, 山口雄輝, 半田宏

    実験医学   Vol. 28   page: 2115 - 2118   2010

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  5. Only posterior interdigit provides positional information to its anterior PFR to specify each digit identity International coauthorship International journal

    Takayuki Suzuki, Sean M. Hasso, Toshihiko Ogura, John F. Fallon

    DEVELOPMENTAL BIOLOGY   Vol. 319 ( 2 ) page: 597 - 597   2008.7

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    DOI: 10.1016/j.ydbio.2008.05.516

    Web of Science

  6. Tbx genes specify posterior digit identity through Shh and BMP signaling (vol 6, pg 43, 2004) International coauthorship International journal

    T Suzuki, J Takeuchi, K Koshiba-Takeuchi, T Ogura

    DEVELOPMENTAL CELL   Vol. 8 ( 6 ) page: 971 - 972   2005.6

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    Language:English   Publisher:CELL PRESS  

    DOI: 10.1016/j.devcel.2005.05.010

    Web of Science

  7. Tbx genes specify posterior digit identity through Shh and BMP signaling. International coauthorship International journal

    T Suzuki, J Takeuchi, K Koshiba-Takeuchi, T Ogura

    DEVELOPMENTAL BIOLOGY   Vol. 259 ( 2 ) page: 581 - 581   2003.7

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    Web of Science

  8. BMP gradient and Tbx genes determine the dorsal-ventral polarity of eye. International coauthorship International journal

    K Koshiba-Takeuchi, J Takeuchi, T Suzuki, T Ogura

    DEVELOPMENTAL BIOLOGY   Vol. 247 ( 2 ) page: 485 - 486   2002.7

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    Web of Science

  9. Tbx genes and the digit identity. International coauthorship International journal

    T Suzuki, J Takeuchi, K Koshiba-Takeuchi, T Ogura

    DEVELOPMENTAL BIOLOGY   Vol. 247 ( 2 ) page: 493 - 493   2002.7

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Web of Science

  10. チロシンホスファターゼ阻害剤 International coauthorship International journal

    梅沢一夫, 鈴木孝幸

    蛋白質核酸酵素増刊号   Vol. 45   page: 852 - 858   2000

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    Language:Japanese   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)  

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Presentations 20

  1. ニワトリ胚を用いた手足の発生に必要な遺伝子の探索と機能解析 Invited International coauthorship International conference

    鈴木孝幸

    愛知県立一宮高校スーパーサイエンスハイスクール特別講義  2020.12.15 

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  2. 四肢動物における後ろ足の位置の多様性を生み出した発生メカニズム Invited International coauthorship International conference

    鈴木 孝幸

    鳥取大学特別講義  2019.10.10 

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  3. 遺伝学・発生学を用いた四肢のパターン形成メカニズムの研究 Invited International coauthorship

    鈴木 孝幸

    日本遺伝学会第91回大会  2019.9.12 

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  4. 四肢動物における仙椎ー後肢ユニットの位置の普遍性と種間における位置の多様性を生み出した発生メカニズム Invited International coauthorship International conference

    鈴木孝幸

    第39回 肉眼解剖学懇話会  2019.3.26 

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  5. ニワトリ胚を用いた脊椎動物における後ろ足の位置の多様性が生み出されたメカニズムの解明 Invited International coauthorship International conference

    鈴木 孝幸

    愛知県立一宮高校スーパーサイエンスハイスクール特別講義  2018.12.18 

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    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  6. 四肢動物における仙椎-後肢ユニットの位置の普遍性と種間における位置の多様性を生み出した発生メカニズム Invited International coauthorship International conference

    SUZUKI Takayuki

    2018.12.11 

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  7. ヘビの胴体なぜ長い?〜脊椎動物の後ろ足の位置の進化のメカニズム〜 Invited International coauthorship International conference

    鈴木 孝幸

    豊橋市自然史博物館 名古屋大学出前事業  2018.12.9 

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  8. ニワトリ胚を用いた四肢動物における後肢の位置の多様性を生み出した発生メカニズム Invited International coauthorship

    SUZUKI Takayuki

    2018.12.8 

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  9. ニワトリ胚を用いた四肢動物における後肢の位置の多様性を生み出す発生メカニズム Invited International coauthorship International conference

    SUZUKI Takayuki

    2018.12.1 

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  10. Heterochrony in initiation of Gdf11 expression specifies unique hindlimb positioning through coordination of Hox gene expression in tetrapods International coauthorship International conference

    SUZUKI Takayuki

    2018.6.8 

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  11. Anatomical integration of the sacral-hindlimb unit coordinated by secreted factor, GDF11, underlies variation in hindlimb positioning in tetrapods Invited International coauthorship

    SUZUKI Takayuki

    2017.11.29 

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  12. 四肢動物における後肢の位置の多様性を生み出す発生メカニズム International coauthorship

    SUZUKI Takayuki

    2017.8.25 

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  13. 脊椎動物における後肢の位置の多様性が生み出される分子メカニズム International coauthorship

    鈴木 孝幸

    第9回 Evo-devo青年の会 特別講演  2016.6.25 

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  14. Quantitative approach to understand whole organ deformation dynamics in the chick limb International coauthorship International conference

    SUZUKI Takayuki

    Avian Model systems 9  2016.3.29 

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  15. 脊椎動物における後肢の位置の多様性を生み出す分子メカニズム Invited International coauthorship

    鈴木 孝幸

    第3回萌える生物学  2015.9.21 

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  16. 肢芽全体の形態形成の定量的解析方法と位置の多様性を生み出す分子メカニズム Invited International coauthorship

    鈴木 孝幸

    第5回 Tokyo Vertebrate Morphology Meeting  2015.8.12 

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  17. Quantification of special and temporal deformation dynamics to understand whole organ development Invited International coauthorship International conference

    SUZUKI Takayuki

    Biological time and biological space  2014.8.27 

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  18. Quantitative approach to understand whole organ morphogenesis Invited International coauthorship International conference

    SUZUKI Takayuki

    2013.5.28 

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  19. Role of GDF11 during hindlimb field determination Invited International coauthorship International conference

    SUZUKI Takayuki

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  20. 発光顕微鏡を用いた肢芽におけるMorphogenシグナルの定量化と形態形成 Invited International coauthorship

    鈴木 孝幸

    第85回日本生化学会大会 

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KAKENHI (Grants-in-Aid for Scientific Research) 11

  1. 仙椎-後肢ユニットの形態の制約と個体間の位置のゆらぎを生み出す分子機構の解明

    Grant number:20H04867  2020.4 - 2022.3

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    鈴木 孝幸

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\10270000 ( Direct Cost: \7900000 、 Indirect Cost:\2370000 )

    本研究は、四肢動物間で不可変である仙椎-後肢ユニットを維持するためのGDF11による5’Hox遺伝子群の入れ子状の発現誘導機構と、誘導された5’Hox遺伝子群の発現が個体間でゆらぎ、仙椎-後肢ユニットの位置自体が個体間でゆらぐ分子機構の両方を明らかにする事を目指す。これにより椎式のアルシャラクシス的な進化を誘導しえた発生メカニズムの実体も明らかにしたい。

  2. Elucidation of molecular mechanisms that brought about positional diversity of the hindlimb in tetrapod

    Grant number:18H02487  2018.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\17420000 ( Direct Cost: \13400000 、 Indirect Cost:\4020000 )

  3. Elucidation of onset of hindlimb initiation by specific somite-stage.

    Grant number:17H05764  2017.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\9360000 ( Direct Cost: \7200000 、 Indirect Cost:\2160000 )

  4. モルフォゲンに依存しない上皮の配向した力学的拘束による肢芽の伸長機構の解明

    2016.4 - 2018.3

    科学研究費補助金  新学術領域研究

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    Authorship:Principal investigator 

  5. マクロからミクロへ:トップダウンアプローチによる階層を超える形態形成の理解

    2015.4 - 2018.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Competitive

  6. Developmental mechanisms that specify digit identity and patterning

    Grant number:25291050  2013.4 - 2016.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Suzuki Takayuki, MORISHITA Yoshihiro

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\18200000 ( Direct Cost: \14000000 、 Indirect Cost:\4200000 )

    Our skeletal pattern is derived from pattern of chondrocyte development. Therefore, studying first developmental step of chondrocyte should give us the most valuable information about positional information of skeletal pattern. In this study, we used chick hindlimb bud as a model system and performed how chondrocyte position is specified in the autopod. We succeeded to establish new quantification method to understand 3-dimentional structure of developing organ, especially limb bud in this study.

  7. 肢芽をモデルとした細胞集団から形態形成をつなぐロジックの解明

    Grant number:23127504  2011.4 - 2013.3

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    鈴木 孝幸

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\9360000 ( Direct Cost: \7200000 、 Indirect Cost:\2160000 )

    発生生物学の究極の課題の一つは何故私たちの体がこのような形をしているのか、というマクロな形作りのメカニズムを解明することである。
    近年の顕微鏡技術の進展により、1細胞レベルで組織の一部の細胞挙動を長期間追跡することが可能になって来た。しかしながら器官全体の形を理解する方法は未だにない。この原因は多くの脊椎動物の器官がそうであるように細胞数が多い事と、組織に厚みがあるからである。これまでの研究では上皮組織などの細胞が単層構造である組織の一部の形の変化が研究されて来た。今後は本研究領域であるミクロな細胞レベルからどのようにしてマクロな形態が構築されて行くのかを解析する新たな方法論の開発が必要である。
    そこで本研究では、組織レベルから器官全体(数百万細胞からなる巨大な器官)の形を理解するための新しい解析量を提案した。具体的な方法として、まずあえて1細胞レベルの細胞の挙動の計測を無視し、数十個単位の組織を多点でラベルした。その後、単位時間当たりのラベルされた組織間の形の変形と細胞増殖率を計算した。その結果を元に次に、“変形の異方性”という組織がどれだけ単位時間当たりに偏った変形をするのかという量を計算した。この特徴量は、どのようにして器官全体のうちの各組織がバイアスして変形しているのかが分かるので、器官全体の形が出来上がる仕組みを定量的に、さらには視覚的に理解する事ができる。これは例えて言うと、これまでの木を見て森を見ず、という解析から森全体の形を見てから木を見ようというアイディアである。このように本研究の結果から、組織から器官全体の形を議論出来る新しい方法論を構築することが出来た。
    本解析方法は、今後再生医療の現場などで、どのようにして再生臓器を正確に試験管内で構築して行くのかを考える時の基礎データとなることが期待される。

  8. Functional analysis of the PFR during digit development

    Grant number:23770244  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    SUZUKI Takayuki, MORISHITA Yoshihiro

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

    We found that the cells located distal to the digital lay received positional information from inter digit. These cells get BMP signaling from adjacent interdigit located at the posterior side. I named this region PFR. Surprisingly, PFR cells have unidirectional sensitivity which means PFR cells respond to BMP signaling coming from only posterior interdigit. In this study, we focused on the origin of the interdigit that sends positional information to the PFR. We have developed a novel method that combines snapshot lineage tracing with Bayesian statistical estimation to construct whole-organ deformation maps from landmark data on limited numbers of space-time points. We applied it to chick limb development. A quantitative tissue deformation map for St.23 and St.24 has been constructed, and its precision has been proven by evaluating its predictive performance. Thus, our method enables deformation dynamics analysis in organogenesis using practical lineage marking techniques.

  9. Role of PFR cells during digit identity determination.

    Grant number:21770226  2009 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    SUZUKI Takayuki

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    We found that the cells located distal to the digital lay received positional information from interdigit. These cells get BMP signaling from adjacent interdigit located at the posterior side. I named this region 'PFR'. We found that digit identity is controlled by physical flow in the interdigit, and this conclusion brings us new insight how morphogen gradient works in the embryos. Further, we identified single point mutation in Silkie chicken that causes preaxial polydactyly in the leg. We will publish this data in this May, in press now

  10. Functional analysis of the PFR as a new signaling center for specifying each digit identity

    Grant number:19870001  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (Start-up)  Grant-in-Aid for Young Scientists (Start-up)

    SUZUKI Takayuki

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\3125000 ( Direct Cost: \2720000 、 Indirect Cost:\405000 )

  11. 指の個性の決定とTbx遺伝子群

    Grant number:03J01713  2003 - 2004

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 孝幸

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\1800000 ( Direct Cost: \1800000 )

    昨年度に申請者は後側の指の個性を決定する因子を特定し発表した。そこで本年度は残る前側の指の個性を決定する因子を探索、解析した。この解析をするにあたりアメリカ合衆国University of WisconsinのJohn F.Fallon研で2004年6月8日から2005年3月31日までの間研究を行った。
    University of WisconsinのXin Sun博士との共同研究でTbx15、Pax9、Lhx9を含む複数の転写因子が指の前側に特異的に発現していることを発見した。現在スクリーニングの段階が終了し、ニワトリを用いた過剰発現を用いてこれらの転写因子の機能を解析中である。また学生とともにニワトリの肢芽で強力に働くRNAiのベクターを開発した。今後はこれらの機能抑制系の実験やノックアウトマウスの作製も合わせて解析していきたい。
    さらにこれまでの実験で申請者はTbx遺伝子群が指間部でのBMPシグナルを介してその前側の指の個性を特徴づけること示した。そこでさらに申請者はいつどこで指間部でのBMPシグナルが指の個性を決めるのか、そのターゲット組織の探索とメカニズムを解析した。その結果指間部でのBMPシグナルの位置価はそれぞれの指間部の中では等しいが、指の個性の決定に必要な組織はそれぞれの指間部の中の一部の前-遠位部であることが判明した。現在その組織がどのようにして指原器に個性を決定するシグナルを与えているのか解析中である。またこれを解析するにあたり未解明の指原器自身の発生のメカニズムも合わせて解析中である。現在指の個性が決定されるまでにSox9、BMPRIB、TGF-β2、Nogginが順番に発現していることを見いだした。今後はこれらのカスケードと指間部でのBMPシグナルの関わりについて生化学的なアプローチを用いて解析していきたいと考えている。

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Teaching Experience (Off-campus) 15

  1. 遺伝学 (B2)

    Nagoya University)

  2. 資源生物科学実験実習 (B3)

    Nagoya University)

  3. 細胞生物学 (B2)

    Nagoya University)

  4. 発生学I (B3)

    Nagoya University)

  5. 生物学実習 (B1)

    Nagoya University)

  6. 生命農学序説

    Nagoya University)

  7. 基礎資源生物科学実験実習 (B2)

    Nagoya University)

  8. 基礎発生学I (B1)

    Nagoya University)

  9. 基礎生物学演習 (B2)

    Nagoya University)

  10. 基礎セミナーB (B1)

    Nagoya University)

  11. 基礎セミナーA (B1)

    Nagoya University)

  12. 動物遺伝学特論 (M1)

    Nagoya University)

  13. G30生物学演習 (英語)(B3)

    Nagoya University)

  14. G30生物学実習(英語)(B3)

    Nagoya University)

  15. G30AB Lab (英語)(B3)

    Nagoya University)

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Media Coverage 10

  1. 朝日新聞掲載

    朝日新聞  2021.3

  2. 日本経済新聞掲載

    日本経済新聞  2020.5

  3. 産経新聞掲載 Newspaper, magazine

    産経新聞  2020.5

  4. 日本経済新聞掲載 TV or radio program

    日本経済新聞  日本経済新聞掲載  2017.9

  5. 読売新聞掲載 TV or radio program

    読売新聞  読売新聞掲載  2017.8

  6. 毎日新聞掲載 Newspaper, magazine

    毎日新聞  毎日新聞掲載  2017.8

  7. 朝日新聞掲載 Newspaper, magazine

    朝日新聞  朝日新聞掲載  2017.8

  8. NHK東海ニュース TV or radio program

    NHK東海  NHK東海ニュース  2017.8

  9. 名古屋テレビ夕方のニュース番組up!にて放送

    名古屋テレビ  名古屋テレビ夕方のニュース番組up!  2017.8

  10. 中日新聞朝刊掲載 Newspaper, magazine

    中日新聞  中日新聞朝刊掲載  2017.8

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