研究者詳細 - 宮田　卓樹

論文 - 宮田　卓樹

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CD206(+) macrophages transventricularly infiltrate the early embryonic cerebral wall to differentiate into microglia. 査読有り国際共著

Hattori Y, Kato D, Murayama F, Koike S, Asai H, Yamasaki A, Naito Y, Kawaguchi A, Konishi H, Prinz M, Masuda T, Wake H, Miyata T

Cell reports 42 巻 ( 2 ) 頁： 112092 - 112092 2023年2月

担当区分：最終著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Cell Reports

The relationships between tissue-resident microglia and early macrophages, especially their lineage segregation outside the yolk sac, have been recently explored, providing a model in which a conversion from macrophages seeds microglia during brain development. However, spatiotemporal evidence to support such microglial seeding in situ and to explain how it occurs has not been obtained. By cell tracking via slice culture, intravital imaging, and Flash tag-mediated or genetic labeling, we find that intraventricular CD206+ macrophages, which are abundantly observed along the inner surface of the mouse cerebral wall, frequently enter the pallium at embryonic day 12. Immunofluorescence of the tracked cells show that postinfiltrative macrophages in the pallium acquire microglial properties while losing the CD206+ macrophage phenotype. We also find that intraventricular macrophages are supplied transepithelially from the roof plate. This study demonstrates that the “roof plate→ventricle→pallium” route is an essential path for microglial colonization into the embryonic mouse brain.

DOI： 10.1016/j.celrep.2023.112092

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Transient microglial absence assists postmigratory neurons in proper differentiation. 査読有り

Hattori Y, Naito Y, Tsugawa Y, Nonaka S, Wake H, Nagasawa T, Kawaguchi A, Miyata T.

Nature Communications 11 巻頁： 1631 2020年4月

Dorsal-to-Ventral Cortical Expansion Is Physically Primed by Ventral Streaming of Early Embryonic Preplate Neurons. 査読有り

Saito K, Okamoto M, Watanabe Y, Noguchi N, Nagasaka A, Nishina Y, Shinoda T, Sakakibara A, Miyata T

Cell Reports 29 巻 ( 6 ) 頁： 1555 - 1567.e5 2019年11月

担当区分：最終著者,　責任著者記述言語：英語出版者・発行元：Cell Reports

Embryonic radial glial fibers that guide most neocortical neurons are ventrally deflected near their terminal, contributing to further expansion of the neocortical area. Saito et al. demonstrate that this fiber deflection is induced physically by a previously unrecognized ventral stream of the earliest generated preplate neurons.

DOI： 10.1016/j.celrep.2019.09.075

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Differentiating cells mechanically limit the interkinetic nuclear migration of progenitor cells to secure apical cytogenesis 査読有り

Watanabe Yuto, Kawaue Takumi, Miyata Takaki

DEVELOPMENT 145 巻 ( 14 ) 2018年7月

Elasticity-based boosting of neuroepithelial nucleokinesis via indirect energy transfer from mother to daughter. 査読有り

Shinoda T, Nagasaka A, Inoue Y, Higuchi R, Minami Y, Kato K, Suzuki M, Kondo T, Kawaue T, Saito K, Ueno N, Fukazawa Y, Nagayama M, Miura T, Adachi T, Miyata T

PLoS Biology 16 巻 ( 4 ) 頁： e2004426 2018年4月

TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding 査読有り

Okamoto, M., Namba, T., Shinoda, T., Kondo, T., Watanabe, T., Inoue, Y., Takeuchi, K., Enomoto, Y., Ota, K., Oda, K., Wada, Y., Sagou, K., Saito, K., Sakakibara, A., Kawaguchi, A., Nakajima, K., Adachi, T., Fujimori, T., Ueda, M. Hayashi, S., Kaibuchi, K., Miyata, T.

Nature Neuroscience 16 巻頁： 1556-1566 2013年9月

Migration, early axonogenesis, and Reelin-dependent layer-forming behavior of early/posterior-born Purkinje cells in the developing mouse lateral cerebellum. 査読有り

Miyata, T., Ono Y, Okamoto M, Masaoka M, Sakakibara A, Kawaguchi A, Hashimoto M, Ogawa M

Neural Development 5 巻 ( 3 ) 頁： 1-21 2010年

Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells. 査読有り

Miyata, T., Kawaguchi, A., Saito, K., Kawano, M., Muto, T., and Ogawa, M.

Development 131 巻頁： 3133-3145 2004年

Asymmetric inheritance of radial glial fibers by cortical neurons. 査読有り

Miyata, T., Kawaguchi, A., Okano, H., and Ogawa, M.

Neuron 31 巻頁： 727-741 2001年

NeuroD is required for differentiation of the granule cells in the cerebellum and hippocampus. 査読有り

Miyata, T., Maeda, T., and Lee, J.E.

Genes & Dev. 13 巻頁： 1647-1652 1999年

Regulation of Purkinje cell alignment by Reelin as revealed with CR-50 antibody. 査読有り

Miyata, T., Nakajima, K., Mikoshiba, K., and Ogawa, M.

J. Neurosci. 17 巻頁： 3599-3609 1997年

Quantitative in toto live imaging analysis of apical nuclear migration in the mouse telencephalic neuroepithelium 査読有り

Shimamura T, Miyata T.

Development, Growth, Differentiation 2024年12月

A novel preparation for histological analyses of intraventricular macrophages in the embryonic brain 査読有り

Futoshi Murayama, Hisa Asai, Arya Kirone Patra, Hiroaki Wake, Takaki Miyata, Yuki Hattori

Development, Growth & Differentiation 2024年6月

Endothelial cells regulate alveolar morphogenesis by constructing basement membranes acting as a scaffold for myofibroblasts 査読有り国際誌

Haruko Watanabe-Takano, Katsuhiro Kato, Eri Oguri-Nakamura, Tomohiro Ishii, Koji Kobayashi, Takahisa Murata, Koichiro Tsujikawa, Takaki Miyata, Yoshiaki Kubota, Yasuyuki Hanada, Koichi Nishiyama, Tetsuro Watabe, Reinhard Fässler, Hirotaka Ishii, Naoki Mochizuki, Shigetomo Fukuhara

Nature Communications 15 巻 ( 1 ) 頁： 1622 2024年3月

Mechanical and physical interactions involving neocortical progenitor cells 招待有り査読有り

Takaki Miyata

Neocortical Neurogenesis in Development and Evolution 頁： 119 - 136 2023年8月

Analyzing the effect of cell rearrangement on Delta-Notch pattern formation

Oguma Toshiki, Takigawa-Imamura Hisako, Shinoda Tomoyasu, Ogura Shuntaro, Uemura Akiyoshi, Miyata Takaki, Maini Philip K., Miura Takashi

PHYSICAL REVIEW E 107 巻 ( 6 ) 頁： 064404 2023年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Physical Review E

The Delta-Notch system plays a vital role in many areas of biology and typically forms a salt and pepper pattern in which cells strongly expressing Delta and cells strongly expressing Notch are alternately aligned via lateral inhibition. In this study, we consider cell rearrangement events, such as cell mixing and proliferation, that alter the spatial structure itself and affect the pattern dynamics. We model cell rearrangement events by a Poisson process and analyze the model while preserving the discrete properties of the spatial structure. We investigate the effects of the intermittent perturbations arising from these cell rearrangement events on the discrete spatial structure itself in the context of pattern formation and by using an analytical approach, coupled with numerical simulation. We find that the homogeneous expression pattern is stabilized if the frequency of cell rearrangement events is sufficiently large. We analytically obtain the balanced frequencies of the cell rearrangement events where the decrease of the pattern amplitude, as a result of cell rearrangement, is balanced by the increase in amplitude due to the Delta-Notch interaction dynamics. Our framework, while applied here to the specific case of the Delta-Notch system, is applicable more widely to other pattern formation mechanisms.

DOI： 10.1103/PhysRevE.107.064404

Web of Science

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その他リンク： http://harvest.aps.org/v2/journals/articles/10.1103/PhysRevE.107.064404/fulltext

Actin crosslinking by α-actinin averts viscous dissipation of myosin force transmission in stress fibers. 査読有り

Katsuta H, Okuda S, Nagayama K, Machiyama H, Kidoaki S, Kato M, Sokabe M, Miyata T, Hirata H

iScience 26 巻 ( 3 ) 頁： 106090 2023年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：iScience

Contractile force generated in actomyosin stress fibers (SFs) is transmitted along SFs to the extracellular matrix (ECM), which contributes to cell migration and sensing of ECM rigidity. In this study, we show that efficient force transmission along SFs relies on actin crosslinking by α-actinin. Upon reduction of α-actinin-mediated crosslinks, the myosin II activity induced flows of actin filaments and myosin II along SFs, leading to a decrease in traction force exertion to ECM. The fluidized SFs maintained their cable integrity probably through enhanced actin polymerization throughout SFs. A computational modeling analysis suggested that lowering the density of actin crosslinks caused viscous slippage of actin filaments in SFs and, thereby, dissipated myosin-generated force transmitting along SFs. As a cellular scale outcome, α-actinin depletion attenuated the ECM-rigidity-dependent difference in cell migration speed, which suggested that α-actinin-modulated SF mechanics is involved in the cellular response to ECM rigidity.

DOI： 10.1016/j.isci.2023.106090

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Functional Cooperation of α-Synuclein and Tau Is Essential for Proper Corticogenesis. 査読有り

Wang S, Fu Y, Miyata T, Matsumoto S, Shinoda T, Itoh K, Harada A, Hirotsune S, Jin M

The Journal of neuroscience : the official journal of the Society for Neuroscience 42 巻 ( 37 ) 頁： 7031 - 7046 2022年9月

記述言語：英語出版者・発行元：Journal of Neuroscience

Alpha-synuclein (aSyn) and tau are abundant multifunctional neuronal proteins, and their intracellular deposits have been linked to many neurodegenerative diseases, including Alzheimer’s disease and Parkinson’s disease. Despite the disease relevance, their physiological roles remain elusive, as mice with knock-out of either of these genes do not exhibit overt phenotypes. To reveal functional cooperation, we generated aSyn2/2tau2/2 double-knock-out mice and characterized the functional cross talk between these proteins during brain development. Intriguingly, deletion of aSyn and tau reduced Notch signaling and accelerated interkinetic nuclear migration of G2 phase at early embryonic stage. This significantly altered the balance between the proliferative and neurogenic divisions of progenitor cells, resulting in an overproduction of early born neurons and enhanced neurogenesis, by which the brain size was enlarged during the embryonic stage in both sexes. On the other hand, a reduction in the number of neural progenitor cells in the middle stage of corticogenesis diminished subsequent gliogenesis in the aSyn2/2tau2/2 cortex. Additionally, the expansion and maturation of macroglial cells (astrocytes and oligodendrocytes) were suppressed in the aSyn2/2tau2/2 postnatal brain, which in turn reduced the male aSyn2/2tau2/2 brain size and cortical thickness to less than the control values. Our study identifies important functional cooperation of aSyn and tau during corticogenesis.

DOI： 10.1523/JNEUROSCI.0396-22.2022

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Border-associated macrophages transventricularly infiltrate the early embryonic cerebral wall to differentiate into microglia

Yuki Hattori, Daisuke Kato, Futoshi Murayama, Sota Koike, Yu Naito, Ayano Kawaguchi, Hiroaki Wake, Takaki Miyata

2022年7月

出版者・発行元：Cold Spring Harbor Laboratory

Summary

The relationships between microglia and macrophages, especially their lineage segregation outside the yolk sac, have been recently explored, providing a model in which a conversion from macrophages seeds microglia during brain development. However, spatiotemporal evidence to support such microglial seeding and to explain how it occurs has not been obtained. By cell tracking via slice culture, intravital imaging, and Flash tag-mediated labeling, we found that a group of intraventricular macrophages belonging to border-associated macrophages (BAMs), which were abundantly observed along the inner surface of the mouse cerebral wall at embryonic day 12, frequently entered the brain wall. Immunohistochemistry of the tracked cells showed that postinfiltrative BAMs acquired microglial properties while losing a macrophage phenotype. We also found that the intraventricular BAMs were supplied transepithelially from the roof plate. Thus, this study demonstrates that the “roof plate→ventricle→cerebral wall” route is an essential path for microglial colonization into the embryonic mouse brain.

DOI： 10.1101/2022.07.27.501563

Mid1 is associated with androgen-dependent axonal vulnerability of motor neurons in spinal and bulbar muscular atrophy. 査読有り

Ogura Y, Sahashi K, Hirunagi T, Iida M, Miyata T, Katsuno M

Cell death & disease 13 巻 ( 7 ) 頁： 601 2022年7月

記述言語：英語出版者・発行元：Cell Death and Disease

Spinal and bulbar muscular atrophy (SBMA) is an adult-onset hereditary neurodegenerative disease caused by the expansions of CAG repeats in the androgen receptor (AR) gene. Androgen-dependent nuclear accumulation of pathogenic AR protein causes degeneration of lower motor neurons, leading to progressive muscle weakness and atrophy. While the successful induction of SBMA-like pathology has been achieved in mouse models, mechanisms underlying motor neuron vulnerability remain unclear. In the present study, we performed a transcriptome-based screening for genes expressed exclusively in motor neurons and dysregulated in the spinal cord of SBMA mice. We found upregulation of Mid1 encoding a microtubule-associated RNA binding protein which facilitates the translation of CAG-expanded mRNAs. Based on the finding that lower motor neurons begin expressing Mid1 during embryonic stages, we developed an organotypic slice culture system of the spinal cord obtained from SBMA mouse fetuses to study the pathogenic role of Mid1 in SBMA motor neurons. Impairment of axonal regeneration arose in the spinal cord culture in SBMA mice in an androgen-dependent manner, but not in mice with non-CAG-expanded AR, and was either exacerbated or ameliorated by Mid1 overexpression or knockdown, respectively. Hence, an early Mid1 expression confers vulnerability to motor neurons, at least by inducing axonogenesis defects, in SBMA.

DOI： 10.1038/s41419-022-05001-6

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PubMed

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