Nagoya University   Assistant

2007.1 - 2007.3

Doshisha University

2006.4 - 2006.12

International Society for Extracellular Vesicles

Neuritogenic steroid glycosides from crown-of-thorns starfish: Possible involvement of p38 mitogen-activated protein kinase and attenuation of cognitive impairment in senescence-accelerated mice (SAMP8) by peripheral administration Reviewed International coauthorship International journal

Sasayama Yumi, Mamiya Takayoshi, Qi Jianhua, Shibata Takahiro, Uchida Koji, Nabeshima Toshitaka, Ojika Makoto

BIOORGANIC & MEDICINAL CHEMISTRY   Vol. 78   page： 117144   2023.1

Metabolomics of small extracellular vesicles derived from isocitrate dehydrogenase 1-mutant HCT116 cells collected by semi-automated size exclusion chromatography Invited Reviewed International journal

Hayasaka Ryosuke, Tabata Sho, Hasebe Masako, Ikeda Satsuki, Hikita Tomoya, Oneyama Chitose, Yoshitake Jun, Onoshima Daisuke, Takahashi Kumiko, Shibata Takahiro, Uchida Koji, Baba Yoshinobu, Soga Tomoyoshi, Tomita Masaru, Hirayama Akiyoshi

FRONTIERS IN MOLECULAR BIOSCIENCES   Vol. 9   page： 1049402   2023.1

Tetrachloroaurate (III)–induced oxidation increases nonthermal plasma-induced aldehydes Invited Reviewed

3. Okazaki Y, Yoshitake J, Ito N, Sasaki K, Tanaka H, Hori M, Shibata T, Toyokuni S.

Adv. Redox Res.   Vol. 9   page： 10004   2023

Molecular networking-based lipid profiling and multi-omics approaches reveal new contributions of functional vanilloids to gut microbiota and lipometabolism changes Reviewed International journal

Hattori Hiroyuki, Moriyama Akihiro, Ohno Tomoki, Shibata Takahiro, Iwahashi Hitoshi, Mitsunaga Tohru

FOOD CHEMISTRY: MOLECULAR SCIENCES   Vol. 5   page： 100123   2022.12

Autoxidation of ascorbate mediates lysine N-pyrrolation Reviewed International journal

Yoshitake Jun, Shibata Takahiro, Chikazawa Miho, Uchida Koji

FREE RADICAL RESEARCH   Vol. 56 ( 11-12 ) page： 749 - 759   2022.12

Rapid Isolation of Extracellular Vesicles Using a Hydrophilic Porous Silica Gel-Based Size-Exclusion Chromatography Column Reviewed

Yoshitake Jun, Azami Mayuko, Sei Haruka, Onoshima Daisuke, Takahashi Kumiko, Hirayama Akiyoshi, Uchida Koji, Baba Yoshinobu, Shibata Takahiro

ANALYTICAL CHEMISTRY   Vol. 94 ( 40 ) page： 13676 - 13681   2022.9

Histone functions as a cell-surface receptor for AGEs Reviewed

Itakura Masanori, Yamaguchi Kosuke, Kitazawa Roma, Lim Sei-Young, Anan Yusuke, Yoshitake Jun, Shibata Takahiro, Negishi Lumi, Sugawa Hikari, Nagai Ryoji, Uchida Koji

NATURE COMMUNICATIONS   Vol. 13 ( 1 ) page： 2974   2022.5

Redox-dependent internalization of the purinergic P2Y(6) receptor limits colitis progression Reviewed International coauthorship

Nishiyama Kazuhiro, Nishimura Akiyuki, Shimoda Kakeru, Tanaka Tomohiro, Kato Yuri, Shibata Takahiro, Tanaka Hiroshi, Kurose Hitoshi, Azuma Yasu-Taka, Ihara Hideshi, Kumagai Yoshito, Akaike Takaaki, Eaton Philip, Uchida Koji, Nishida Motohiro

SCIENCE SIGNALING   Vol. 15 ( 716 ) page： eabj0644   2022.1

Agonist/Antagonist Activity of Oxytocin Variants Obtained from Free Cyclic Peptide Libraries Generated via Amino Acid Substitution Reviewed

Kinoshita Remi, Kozaki Ikko, Shimizu Kazunori, Shibata Takahiro, Ochiai Akihito, Honda Hiroyuki

ACS OMEGA   Vol. 6 ( 46 ) page： 31244 - 31252   2021.11

Low-molecular-weight whey proteins promote collagen production in dermal fibroblasts via the TGF-beta receptor/Smad pathway Reviewed

Katayoshi Takeshi, Kusano Yuri, Shibata Takahiro, Uchida Koji, Tsuji-Naito Kentaro

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 85 ( 11 ) page： 2232 - 2240   2021.11

Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified Reviewed

Tanaka Hiromasa, Hosoi Yugo, Ishikawa Kenji, Yoshitake Jun, Shibata Takahiro, Uchida Koji, Hashizume Hiroshi, Mizuno Masaaki, Okazaki Yasumasa, Toyokuni Shinya, Nakamura Kae, Kajiyama Hiroaki, Kikkawa Fumitaka, Hori Masaru

SCIENTIFIC REPORTS   Vol. 11 ( 1 ) page： 18488   2021.9

Oxidative deamination of lysine residues by polyphenols generates an equilibrium of aldehyde and 2-piperidinol products Reviewed

Yamaguchi Kosuke, Itakura Masanori, Kitazawa Roma, Lim Sei-Young, Nagata Koji, Shibata Takahiro, Akagawa Mitsugu, Uchida Koji

JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 297 ( 3 ) page： 101035   2021.9

6-Paradol Acts as a Potential Anti-obesity Vanilloid from Grains of Paradise Reviewed International journal

Hattori Hiroyuki, Mori Takashi, Shibata Takahiro, Kita Masaki, Mitsunaga Tohru

MOLECULAR NUTRITION & FOOD RESEARCH   Vol. 65 ( 16 ) page： e2100185   2021.8

Identification of biosynthetic intermediates for the mating hormone alpha 2 of the plant pathogen Phytophthora Reviewed

Ariyoshi Suguru, Imazu Yusuke, Ohguri Ryuji, Katsuta Ryo, Yajima Arata, Shibata Takahiro, Ojika Makoto

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 85 ( 8 ) page： 1802 - 1808   2021.7

Sodium sulfite causes gastric mucosal cell death by inducing oxidative stress Reviewed

Oshimo Moeri, Nakashima Fumie, Kai Kenji, Matsui Hirofumi, Shibata Takahiro, Akagawa Mitsugu

FREE RADICAL RESEARCH   Vol. 55 ( 6 ) page： 731 - 743   2021.6

Screening of a novel free fatty acid receptor 1 (FFAR1) agonist peptide by phage display and machine learning based-amino acid substitution Reviewed

Yoshioka Keitaro, Yamashita Haruki, Shimizu Kazunori, Shimomura Sayako, Shibata Takahiro, Miyazaki Jun-ichi, Honda Hiroyuki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 550   page： 177 - 183   2021.4

Extracellular vesicles derived from inflamed murine colorectal tissue induce fibroblast proliferation via epidermal growth factor receptor

Hasegawa Kana, Kuwata Keiko, Yoshitake Jun, Shimomura Sayako, Uchida Koji, Shibata Takahiro

FEBS JOURNAL   Vol. 288 ( 6 ) page： 1906 - 1917   2021.3

Screening of a novel free fatty acid receptor 1 (FFAR1) agonist peptide by phage display and machine learning based-amino acid substitution. Invited Reviewed

Yoshioka K, Yamashita H, Shimizu K, Shimomura S, Shibata T, Miyazaki JI, Honda H.

Biochem. Biophys. Res. Commun.   Vol. 550   page： 177 - 183   2021

PPAR alpha Ligand-Binding Domain Structures with Endogenous Fatty Acids and Fibrates

Kamata Shotaro, Oyama Takuji, Saito Kenta, Honda Akihiro, Yamamoto Yume, Suda Keisuke, Ishikawa Ryo, Itoh Toshimasa, Watanabe Yasuo, Shibata Takahiro, Uchida Koji, Suematsu Makoto, Ishii Isao

ISCIENCE   Vol. 23 ( 11 ) page： 101727   2020.11

Acrolein in cigarette smoke attenuates the innate immune responses mediated by surfactant protein D

Takamiya Rina, Takahashi Motoko, Maeno Toshitaka, Saito Atsushi, Kato Masaki, Shibata Takahiro, Uchida Koji, Ariki Shigeru, Nakano Miyako

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS   Vol. 1864 ( 11 ) page： 129699   2020.11

Acyl-CoA dehydrogenase long chain (ACADL) is a target protein of stylissatin A, an anti-inflammatory cyclic heptapeptide

Zhang Menghua, Sunaba Taiki, Sun Yiting, Shibata Takahiro, Sasaki Kazunori, Isoda Hiroko, Kigoshi Hideo, Kita Masaki

JOURNAL OF ANTIBIOTICS   Vol. 73 ( 8 ) page： 589 - 592   2020.8

Isolation and structure-activity relationship studies of jacaranones: Anti-inflammatory quinoids from the Cuban endemic plant Jacaranda arborea (Bignoniaceae)

Hirukawa Minako, Zhang Menghua, Echenique-Diaz Lazaro M., Mizota Koji, Ohdachi Satoshi D., Begue-Quiala Gerardo, Delgado-Labanino Jorge L., Gamez-Diez Jorgelino, Alvarez-Lemus Jose, Galano Machado Leandro, Suarez Nunez Miguel, Shibata Takahiro, Kigoshi Hideo, Kita Masaki

Tetrahedron Letters   Vol. 61 ( 26 )   2020.6

Glycolaldehyde is an endogenous source of lysine N-pyrrolation

Chikazawa Miho, Yoshitake Jun, Lim Sei-Young, Iwata Shiori, Negishi Lumi, Shibata Takahiro, Uchida Koji

JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 295 ( 22 ) page： 7697 - 7709   2020.5

Quantitative analysis of oxidized vitamin B1 metabolites generated by hypochlorous acid

Sasatsuki Hitoshi, Nakazaki Atsuo, Uchida Koji, Shibata Takahiro

FREE RADICAL BIOLOGY AND MEDICINE   Vol. 152   page： 197 - 206   2020.5

Relationship among structure, cytotoxicity, and Michael acceptor reactivity of quinocidin

Nakagawa Yu, Sawaki Yuki, Miyanishi Wataru, Shimomura Sayako, Shibata Takahiro, Ojika Makoto

BIOORGANIC & MEDICINAL CHEMISTRY   Vol. 28 ( 4 ) page： 115308   2020.2

A unique mechanism for thiolation of serum albumins by disulphide molecules

Nakashima Fumie, Shibata Takahiro, Uchida Koji

JOURNAL OF BIOCHEMISTRY   Vol. 167 ( 2 ) page： 165 - 171   2020.2

Redox cycling of 9,10-phenanthrenequinone activates epidermal growth factor receptor signaling through S-oxidation of protein tyrosine phosphatase 1B

Nho Cong Luong, Abiko Yumi, Shibata Takahiro, Uchida Koji, Warabi Eiji, Suzuki Midori, Noguchi Takuya, Matsuzawa Atsushi, Kumagai Yoshito

JOURNAL OF TOXICOLOGICAL SCIENCES   Vol. 45 ( 6 ) page： 349 - 363   2020

Protein adductomics: A comprehensive analysis of protein modifications by electrophiles.

Shibata T, Uchida K

Free radical biology & medicine   Vol. 144   page： 218 - 222   2019.11

Modification of endothelial nitric oxide synthase by 4-oxo-2(E)-nonenal(ONE) in preeclamptic placentas. Reviewed International coauthorship International journal

Free radical biology & medicine   Vol. 141   page： 416 - 425   2019.9

A Dual Perspective of the Action of Lysine on Soybean Oil Oxidation Process Obtained by Combining ¹H NMR and LC-MS: Antioxidant Effect and Generation of Lysine-Aldehyde Adducts. Reviewed International coauthorship International journal

Antioxidants (Basel, Switzerland)   Vol. 8 ( 9 )   2019.9

Molecular Mechanism of Cellular Oxidative Stress Sensing by Keap1

Suzuki Takafumi, Muramatsu Aki, Saito Ryota, Iso Tatsuro, Shibata Takahiro, Kuwata Keiko, Kawaguchi Shin-ichi, Iwawaki Takao, Adachi Saki, Suda Hiromi, Morita Masanobu, Uchida Koji, Baird Liam, Yamamoto Masayuki

CELL REPORTS   Vol. 28 ( 3 ) page： 746 - +   2019.7

Apolipoprotein E binds to and reduces serum levels of DNA-mimicking, pyrrolated proteins

Hirose Sayumi, Hioki Yusuke, Miyashita Hiroaki, Hirade Naoya, Yoshitake Jun, Shibata Takahiro, Kikuchi Ryosuke, Matsushita Tadashi, Chikazawa Miho, Itakura Masanori, Zhang Mimin, Nagata Koji, Uchida Koji

JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 294 ( 28 ) page： 11035 - 11045   2019.7

2-Alkenal modification of hemoglobin: Identification of a novel hemoglobin-specific alkanoic acid-histidine adduct. Reviewed International journal

Yoshitake J, Shibata T, Shimayama C, Uchida K

Redox biology   Vol. 23   page： 101115   2019.5

2-Oxo-histidine-containing dipeptides are functional oxidation products

Ihara Hideshi, Kakihana Yuki, Yamakage Akane, Kai Kenji, Shibata Takahiro, Nishida Motohiro, Yamada Ken-ichi, Uchida Koji

JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 294 ( 4 ) page： 1279-1289   2019.1

Diosgenin Supplementation Prevents Lipid Accumulation and Induces Skeletal Muscle-Fiber Hypertrophy in Rats

Kusano Yuri, Tsujihara Nobuko, Masui Hironori, Shibata Takahiro, Uchida Koji, Takeuchi Wakako

JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY   Vol. 65 ( 5 ) page： 421 - 429   2019

Development of a novel monoclonal antibody against 4-hydroxy-2E,6Z-dodecadienal (4-HDDE)-protein adducts: Immunochemical application in quantitative and qualitative analyses of lipid peroxidation in vitro and ex vivo

Uchida Koji, Shibata Takahiro, Toyokuni Shinya, Daniel Bareket, Zarkovic Kamelija, Zarkovic Neven, Sasson Shlomo

FREE RADICAL BIOLOGY AND MEDICINE   Vol. 124   page： 12-20   2018.8

Structural and functional insights into S-thiolation of human serum albumins

Nakashima Fumie, Shibata Takahiro, Kamiya Kohei, Yoshitake Jun, Kikuchi Ryosuke, Matsushita Tadashi, Ishii Isao, Gimenez-Bastida Juan A., Schneider Claus, Uchida Koji

SCIENTIFIC REPORTS   Vol. 8 ( 1 ) page： 932   2018.1

Detection and quantification of 2-oxo-histidine-containing dipeptides

Ihara Hideshi, Kakihana Yuki, Yamakage Akane, Shibata Takahiro, Uchida Koji

FREE RADICAL BIOLOGY AND MEDICINE   Vol. 112   page： 26 - 27   2017.11

Lipid radicals cause light-induced retinal degeneration Reviewed International journal

Enoki Masataka, Shinto Saki, Matsuoka Yuta, Otsuka Ayasa, Kaidzu Sachiko, Tanito Masaki, Shibata Takahiro, Uchida Koji, Ohira Akihiro, Yamatoa Mayumi, Yamada Ken-ichi

CHEMICAL COMMUNICATIONS   Vol. 53 ( 79 ) page： 10922 - 10925   2017.10

Identification of Polyphenol-Specific Innate Epitopes That Originated from a Resveratrol Analogue

Furuhashi Mai, Hatasa Yukinori, Kawarnura Sae, Shibata Takahiro, Akagawa Mitsugu, Uchida Koji

BIOCHEMISTRY   Vol. 56 ( 35 ) page： 4701-4712   2017.9

De Novo Synthesis of Possible Candidates for the Inagami-Tamura Endogenous Digitalis-like Factor

Nakazaki Atsuo, Hashimoto Keiko, Ikeda Ai, Shibata Takahiro, Nishikawa Toshio

JOURNAL OF ORGANIC CHEMISTRY   Vol. 82 ( 17 ) page： 9097-9111   2017.9

Disruption of the structural and functional features of surfactant protein A by acrolein in cigarette smoke

Takamiya Rina, Uchida Koji, Shibata Takahiro, Maeno Toshitaka, Kato Masaki, Yamaguchi Yoshiki, Ariki Shigeru, Hasegawa Yoshihiro, Saito Atsushi, Miwa Soichi, Takahashi Hiroki, Akaike Takaaki, Kuroki Yoshio, Takahashi Motoko

SCIENTIFIC REPORTS   Vol. 7 ( 1 ) page： 8304   2017.8

Adductome-based identification of biomarkers for lipid peroxidation

Shibata Takahiro, Shimizu Kazuma, Hirano Keita, Nakashima Fumie, Kikuchi Ryosuke, Matsushita Tadashi, Uchida Koji

JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 292 ( 20 ) page： 8223-8235   2017.5

Oxidative metabolism of curcumin-glucuronide by peroxidases and isolated human leukocytes

Luis Paula B., Gordon Odaine N., Nakashima Fumie, Joseph Akil I., Shibata Takahiro, Uchida Koji, Schneider Claus

BIOCHEMICAL PHARMACOLOGY   Vol. 132   page： 143-149   2017.5

Roles of 5-lipoxygenase and cyclooxygenase-2 in the biosynthesis of hemiketals E-2 and D-2 by activated human leukocytes

Gimenez-Bastida Juan A., Shibata Takahiro, Uchida Koji, Schneider Claus

FASEB JOURNAL   Vol. 31 ( 5 ) page： 1867-1878   2017.5

An open sandwich immunoassay for detection of 13(R,S)-hydroxy-9(E),11(E)-octadecadienoic acid Reviewed International coauthorship

Dong Jinhua, Shichiri Mototada, Chung Chan-I., Shibata Takahiro, Uchida Koji, Hagihara Yoshihisa, Yoshida Yasukazu, Ueda Hiroshi

ANALYST   Vol. 142 ( 5 ) page： 787 - 793   2017.3

Heat Shock Protein 90 Modulates Lipid Homeostasis by Regulating the Stability and Function of Sterol Regulatory Element-binding Protein (SREBP) and SREBP Cleavage-activating Protein

Kuan Yen-Chou, Hashidume Tsutomu, Shibata Takahiro, Uchida Koji, Shimizu Makoto, Inoue Jun, Sato Ryuichiro

JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 292 ( 7 ) page： 3016-3028   2017.2

Roles of 5-lipoxygenase and cyclooxygenase-2 in the biosynthesis of hemiketals E2 and D2 by activated human leukocytes. Reviewed

Giménez-Bastida, J.A., Shibata, T., Uchida, K., & Schneider, C.

FASEB J.   Vol. ３２   page： 1867-1878   2017

Identification of C1q as a Binding Protein for Advanced Glycation End Products. Reviewed

Chikazawa, M., Shibata, T., Hatasa, Y., Hirose, S., Otaki, N., Nakashima, F., Ito, M., Machida, S., Maruyama, S., & Uchida, K.

  Vol. 55   page： 435-446   2016

Identification of lactate dehydrogenase as a mammalian pyrroloquinoline quinone (PQQ)-binding protein. Reviewed

Akagawa, M., Minematsu, K., Shibata, T., Kondo, T., Ishii, T., & Uchida K.

Sci Rep.   Vol. 6   page： 26723   2016

Oxidative Deamination of Serum Albumins by (-)-Epigallocatechin-3-O-Gallate: A Potential Mechanism for the Formation of Innate Antigens by Antioxidants Reviewed

PLoS One.   Vol. 11   page： e0153002   2016

Identification of a prostaglandin D2 metabolite as a neuritogenesis enhancer targeting the TRPV1 ion channel. Reviewed

Shibata, T., Takahashi, K., Matsubara, Y., Inuzuka, E., Nakashima, F., Takahashi, N., Kozai, D., Mori, Y., & Uchida, K.

Sci. Rep.   Vol. 6   page： 21261   2016

Functional interaction between cyclooxygenase-2 and p53 in response to an endogenous electrophile. Reviewed

Kumagai, T., Usami, H., Matsukawa, N., Nakashima, F., Chikazawa, M., Shibata, T., Noguchi, N., and Uchida, K.

Redox Biol.   Vol. 4   page： 74-86   2015

15-Deoxy-Δ12,14-prostaglandin J2 as an electrophilic mediator. Invited Reviewed

Shibata, T.

Biosci. Biotechnol. Biochem.   Vol. 79   page： 1044-1049   2015

Toll-like receptors as a target of food-derived anti-inflammatory compounds. Reviewed

Shibata, T., Nakashima, F., Honda, K., Lu, Y.J., Kondo, T., Ushida, Y., Aizawa, K., Suganuma, H., Oe, S., Tanaka, H., Takahashi, T., and Uchida, K.

J. Biol. Chem.   Vol. 289   page： 32757-32772.   2014

Lysine pyrrolation is a naturally-occurring covalent modification involved in the production of DNA mimic proteins. Reviewed

Miyashita, H., Chikazawa, M., Otaki, N., Hioki, Y., Shimozu, Y., Nakashima, F., Shibata, T., Hagihara, Y., Maruyama, S., Matsumi, N., Uchida, K. (2014)

Sci. Rep.   Vol. 4   page： 5343   2014

Multispecificity of immunoglobulin M antibodies raised against advanced glycation end products: involvement of electronegative potential of antigens. Reviewed

1. Chikazawa, M., Otaki, N., Shibata, T., Miyashita, H., Kawai, Y., Maruyama, S., Toyokuni, S., Kitaura, Y., Matsuda, T., and Uchida, K.

J. Biol. Chem.   Vol. 288   page： 13204-13214   2013

An apoptosis-associated mammary protein deficiency leads to enhanced production of IgM antibodies against multiple damage-associated molecules. Reviewed

Chikazawa, M., Otaki, N., Shibata, T., Yasueda, T., Matsuda, T., and Uchida, K.

PLoS One   Vol. 8   page： e68468.   2013

Identification of 4-hydroxy-2-nonenal-histidine adducts that serve as ligands for human lectin-like oxidized LDL receptor-1. Reviewed

5. Kumano-Kuramochi, M., Shimozu, Y., Wakita, C., Ohnishi-Kameyama, M., Shibata, T., Matsunaga, S., Takano-Ishikawa, Y., Watanabe, J., Goto, M., Xie, Q., Komba, S., Uchida, K., and Machida, S

Biochem. J.   Vol. 442   page： 171-180   2012.2

Hydrogen sulfide anion regulates redox signaling via electrophile sulfhydration. Reviewed

Nishida M, Sawa T, Kitajima N, Ono K, Inoue H, Ihara H, Motohashi H, Yamamoto M, Suematsu M, Kurose H, van der Vliet A, Freeman BA, Shibata T, Uchida K, Kumagai Y, Akaike T.

  Vol. 8   page： 714- 724   2012

Quantitative Analysis of Acrolein-Specific Adducts Generated during Lipid Peroxidation-Modification of Proteins in Vitro. Reviewed

  Vol. 25   page： 1384-1392   2012

Monoclonal Antibody against Protein-Bound Glutathione: Use of Glutathione Conjugate of Acrolein-Modified Proteins as an Immunogen. Reviewed

Chem. Res. Toxicol.   Vol. 25   page： 1393-1401   2012

Constitutive expression of an antioxidant enzyme, glutathione S-transferase P1, during differentiation of human intestinal Caco-2 cells. Reviewed

Kusano Y, Horie S, Morishita N, Shibata T, Uchida K.

Free Radic. Biol. Med.   Vol. 53   page： 347-356   2012

Transthiocarbamoylation of proteins by thiolated isothiocyanates Reviewed

Shibata, T., Kimura, Y., Mukai, A., Mori, H., Ito, S., Asaka, Y., Oe, S., Tanaka, H., Takahashi, T., and Uchida, K.

J. Biol. Chem.     page： in press   2011

4-Hydroperoxy-2-nonenal is not just an intermediate, but a reactive molecule that covalently modifies proteins to generate unique intramolecular oxidation products. Reviewed

Shimozu, Y., Hirano, K., Shibata, T., Shibata, N., and Uchida, K.

J. Biol. Chem.   Vol. 286   page： 29313-29324.   2011

Lipid peroxidation modification of protein generates Nepsilon-(4-oxononanoyl)lysine as a pro-inflammatory ligand. . Reviewed

Shibata, T., Shimozu, Y., Wakita, C., Shibata, N., Kobayashi, M., Machida, S., Kato, R., Itabe, H., Zhu, X., Sayre, L. M., and Uchida, K.

J. Biol. Chem   Vol. 286   page： 19943-19957.   2011

A method for detection of 4-hydroxy-2-nonenal adducts in proteins. Reviewed

Wakita, C., Honda, K., Shibata, T., Akagawa, M., and Uchida, K.

Free Radic. Biol. Med.   Vol. 51   page： 1-4   2011

Delta12-Prostaglandin J2 as a Product and Ligand of Human Serum Albumin: Formation of an Unusual Covalent Adduct at His146. Reviewed

Yamaguchi, S., Aldini, G., Ito, S., Morishita, N., Shibata, T., Vistoli, G., Carini, M., Uchida, K.

J. Am. Chem. Soc.   Vol. 132   page： 824-832   2010

Identification of a lipid peroxidation product as the source of oxidation-specific epitopes recognized by anti-DNA autoantibodies. Reviewed

Otaki, N., Chikazawa, M., Nagae, R., Shimozu, Y., Shibata, T., Ito, S., Takasaki, Y., Fujii, J., and Uchida K.

J. Biol. Chem.   Vol. 285   page： 33834-33842   2010

Pertussis toxin upregulates angiotensin type 1 receptors through Toll-like receptor 4-mediated Rac activation. Reviewed

Nishida, M., Suda, R., Nagamatsu, Y., Tanabe, S., Onohara, N., Nakaya, M., Kanaho, Y., Shibata, T., Uchida, K., Sumimoto, H., Sato, Y., Kurose, H.

J. Biol. Chem.   Vol. 285   page： 15268-15277   2010

Lipid peroxidation generates a body odor component trans-2-nonenal covalently bound to protein in vivo. Reviewed

Ishino, K., Wakita, C., Shibata, T., Toyokuni, S., Machida, S., Matsuda, S., Matsuda, T., Uchida, K.

J. Biol. Chem.   Vol. 285   page： 15302-15313   2010

Alteration of biochemical and pathological properties of TDP-43 protein by a lipid mediator, 15-deoxy-delta12,14-prostaglandin J2. Reviewed

Zhang, H.X., Tanji, K., Yoshida, H., Hayakari, M., Shibata, T., Mori, F., Uchida, K., Wakabayashi, K.

Exp. Neurol.   Vol. 222   page： 296-303   2010

Lipid peroxidation generates a body odor component trans-2-nonenal covalently bound to protein in vivo. Reviewed

J. Biol. Chem.   ( 285 ) page： 15302-15313   2010

Alteration of biochemical and pathological properties of TDP-43 protein by a lipid mediator, 15-deoxy- delta12,14-prostaglandin J2. Reviewed

30) Zhang, H.X., Tanji, K., Yoshida, H., Hayakari, M., Shibata, T., Mori, F., Uchida, K., Wakabayashi, K.

Exp. Neurol.   Vol. 222   page： 296-303   2010

Identification of advanced reaction products originating from the initial 4-oxo-2-nonenal-cysteine Michael adducts. Reviewed

Shimozu, Y., Shibata, T., Ojika, M., Uchida, K.

Chem. Res. Toxicol.   Vol. 22   page： 957-964   2009

Immunohistochemical Detection of 13(R)-hydroxyoctadecadienoic Acid in Atherosclerotic Plaques of Human Carotid Arteries Using a Novel Specific Antibody. Reviewed

Shibata N, Toi S, Shibata T, Uchida K, Itabe H, Sawada T, Kawamata T, Okada Y, Uchiyama S, Kobayashi M.

Acta Histochem. Cytochem.   Vol. 42   page： 197-203   2009

Stereochemical configuration of 4-hydroxy-2-nonenal-cysteine adducts and their stereoselective formation in a redox-regulated protein. Reviewed

Wakita, C., Maeshima, T., Yamazaki, A., Shibata, T., Ito, S., Akagawa, M., Ojika, M., Yodoi, J., Uchida, K.

J. Biol. Chem.   Vol. 284   page： 28810-28822   2009

15-Deoxy-Delta(12,14)-prostaglandin J2: An Electrophilic Trigger of Cellular Responses. Invited Reviewed

Uchida, K., & Shibata, T.

Chem. Res. Toxicol. 138-144.   Vol. 21   page： 138-144   2008.1

*A food-derived synergist of NGF signaling: Identification of protein tyrosine phosphatase 1B as a key regulator of NGF receptor-initiated signal transduction. Reviewed

Shibata T, Nakahara H, Kita N, Matsubara Y, Han C, Morimitsu Y, Iwamoto N, Kumagai Y, Nishida M, Kurose H, Aoki N, Ojika M, Uchida K.

J. Neurochem.   Vol. 107 ( 5 ) page： 1248-1260   2008

Molecular characterization of TRPA1 channel activation by cysteine-reactive inflammatory mediators. Reviewed

Takahashi, N., Mizuno, Y., Kozai, D., Yamamoto, S., Kiyonaka, S., Shibata, T., Uchida, K., and Mori, Y.

Channels (Austin)   Vol. 2   page： 287-298   2008

Protein N-Acylation: H2O2-Mediated Covalent Modification of Protein by Lipid Peroxidation-Derived Saturated Aldehydes. Reviewed

Ishino, K., Shibata, T., Ishii, T., Liu, Y. T., Toyokuni, S., Zhu, X., Sayre, L. M., and Uchida, K.

Chem. Res. Toxicol.   Vol. 21   page： 1261-1270   2008

Keap1 regulates the constitutive expression of GST A1 during differentiation of Caco-2 cells. Reviewed

21) Kusano, Y., Horie, S., Shibata, T., Satsu, H., Shimizu, M., Hitomi, E., Nishida, M., Kurose, H., Itoh, K., Kobayashi, A., Yamamoto, M., and Uchida, K.

Biochemistry   Vol. 47   page： 6169-6177   2008

Identification of a Serum Component That Regulates Cyclooxygenase-2 Gene Expression in Cooperation with 4-Hydroxy-2-nonenal. Reviewed

Kanayama M, Yamaguchi S, Shibata T, Shibata N, Kobayashi M, Nagai R, Arai H, Takahashi K, Uchida K.

J. Biol. Chem.   Vol. 282 ( 33 ) page： 24166-24174   2007.8

Granulatoside A, a Starfish Steroid Glycoside, Enhances PC12 Cell Neuritogenesis Induced by Nerve Growth Factor through an Activation of MAP Kinase. Reviewed

Qi J, Han C, Sasayama Y, Nakahara H, Shibata T, Uchida K, Ojika M.

ChemMedChem.   Vol. 1   page： 1351-4   2007

Dose-dependent differential regulation of cytokine secretion from macrophages by fractalkine. Reviewed

Mizutani, N., Sakurai, T., Shibata, T., Uchida, K., Fujita, J., Kawashima, R., Kawamura, Y. I., Toyama-Sorimachi, N., Imai, T., & Dohi, T.

J. Immunol.   Vol. 179   page： 7478-87   2007

Protein-bound 4-Hydroxy-2-nonenal: AN ENDOGENOUS TRIGGERING ANTIGEN OF ANTI-DNA RESPONSE. Reviewed

Toyoda K, Nagae R, Akagawa M, Ishino K, Shibata T, Ito S, Shibata N, Yamamoto T, Kobayashi M, Takasaki Y, Matsuda T, Uchida K.

J. Biol. Chem.   Vol. 282 ( 35 ) page： 25769-25778   2007

Identification of Actin as a 15-Deoxy-Delta(12,14)-prostaglandin J(2) Target in Neuroblastoma Cells: Mass Spectrometric, Computational, and Functional Approaches To Investigate the Effect on Cytoskeletal Derangement. Reviewed

Aldini G, Carini M, Vistoli G, Shibata T, Kusano Y, Gamberoni L, Dalle-Donne I, Milzani A, Uchida K.

Biochemistry   Vol. 46 ( 10 ) page： 2707-2718   2007

Polymer-Assisted Solution-Phase Synthesis and Neurite-Outgrowth-Promoting Activity of 15-Deoxy- 12,14-PGJ2 Derivatives Reviewed

Tanaka H, Hasegawa T, Kita N, Nakahara H, Shibata T, Oe S, Ojika M, Uchida K, and Takahashi T.

Chemistry-An Asian Journal   Vol. 1   page： 669-677   2007

ATM activation by a sulfhydryl-reactive inflammatory cyclopentenone prostaglandin. Reviewed

Kobayashi M, Ono H, Mihara K, Tauchi H, Komatsu K, Shibata T, Shimizu H, Uchida K, Yamamoto K.

Genes Cells   Vol. 11 ( 7 ) page： 779-789   2006.7

Prostaglandins from a zoanthid: Paclitaxel-like neurite-degenerating and microtubule-stabilizating activities. Reviewed

Han C, Qi J, Shi X, Sakagami Y, Shibata T, Uchida K, & Ojika M.

Biosci. Biotechnol. Biochem.   Vol. 70   page： 706-11   2006.3

Bispecific abs against modified protein and DNA with oxidized lipids. Reviewed

Akagawa M, Ito S, Toyoda K, Ishii Y, Tatsuda E, Shibata T, Yamaguchi S, Kawai Y, Ishino K, Kishi Y, Adachi T, Tsubata T, Takasaki Y, Hattori N, Matsuda T, Uchida K.

Proc. Natl. Acad. Sci. USA   Vol. 103   page： 6160-5   2006

Oxidative and electrophilic stresses activate Nrf2 through inhibition of ubiquitination activity of Keap1. Reviewed

Kobayashi A, Kang MI, Watai Y, Tong KI, Shibata T, Uchida K, Yamamoto M.

Mol. Cell. Biol.   Vol. 26 ( 1 ) page： 221-229   2006

Metal-catalyzed oxidation of protein-bound dopamine. Reviewed

Akagawa M, Ishii Y, Ishii T, Shibata T, Yotsu-Yamashita M, Suyama K, Uchida K.

Biochemistry   Vol. 45 ( 50 ) page： 15120-8   2006

Ebselen, a Seleno-organic Antioxidant, as an Electrophile Reviewed

Sakurai, T., Kanayama, M., Shibata, T., Itoh, K., Kobayashi, A., Yamamoto, M., & Uchida, K.

Chem. Res. Toxicol.   Vol. 19   page： 1196-204   2006

Differential responses of the Nrf2-Keap1 system to laminar and oscillatory shear stresses in endothelial cells. Reviewed

Hosoya T, Maruyama A, Kang MI, Kawatani Y, Shibata T, Uchida K, Itoh K, & Yamamoto M.

J. Biol. Chem.   Vol. 280   page： 27244-27250   2005

「J2型プロスタグランジン研究の現状」

柴田貴広、内田浩二

日本生化学会誌「生化学」   Vol. 77   page： 1189-1192   2005

「野菜由来イソチオシアネートによる神経細胞分化誘導」

柴田貴広、中原寛子、内田浩二

食品工業   Vol. 48   page： 63-67   2005

「プロスタグランジンJ2の化学と生物　-多様な生理活性とその分子過程-」

柴田貴広、内田浩二

日本農芸化学会誌　「化学と生物」   Vol. 43 ( 1 ) page： 20-27   2005

*Identification of a lipid peroxidation product as a potential trigger of the p53 pathway. Reviewed

Shibata T, Iio K, Kawai Y, Shibata N, Kawaguchi M, Toi S, Kobayashi M, Kobayashi M, Yamamoto K, & Uchida K.

J. Biol. Chem.   Vol. 281   page： 1196-1204   2005

Transcription Factor Nrf2 regulates inflammation by mediating the effect of 15-deoxy-delta12,14-prostaglandin J2. Reviewed

Itoh K, Mochizuki M, Ishii Y, Ishii T, Shibata T, Kawamoto Y, Kelly V, Sekizawa K, Uchida K, & Yamamoto M.

Mol. Cell. Biol.   Vol. 24   page： 36-45   2004

Structural basis of protein-bound endogenous aldehydes : Chemical and immunochemical characterizations of configurational isomers of a 4-hydroxy-2-nonenal-histidine adduct. Reviewed

Hashimoto M, Shibata T, Wasada H, Toyokuni S, & Uchida K.

J. Biol. Chem   Vol. 278   page： 5044-51   2003

An endogenous electrophile that modulates the regulatory mechanism of protein turnover. Reviewed

Biochemistry   Vol. 42   page： 13960-13968   2003

Thioredoxin as a molecular target of cyclopentenone prostaglandins. Reviewed

Shibata T, Yamada T, Ishii T, Kumazawa S, Nakamura H, Masutani H, Yodoi J, & Uchida K

J. Biol.Chem   Vol. 278   page： 26046-54   2003

15-Deoxy-delta12,14-prostaglandin J2: The endogenous electrophile that induces neuronal apoptosis Reviewed

Kondo M, Shibata T, Kumagai T, Osawa T, Shibata N, Kobayashi M, Sasaki S, Iwata M, Noguchi N, and Uchida K.

Proc. Natl. Acad. Sci. USA   Vol. 99 ( 11 ) page： 7367-7372   2002

15-Deoxy-delta12,14-prostaglandin J2: A PROSTAGLANDIN D2 METABOLITE GENERATED DURING INFLAMMATORY PROCESSES Reviewed

Shibata T, Kondo M, Osawa T, Shibata N, Kobayashi M, & Uchida K.

J. Biol. Chem.   Vol. 277 ( 12 ) page： 10459-10466   2002

健康維持に役立つ食の機能の理解と利用 Invited

柴田貴広

産学連携学会第19回大会  2021.6 

レドックスアダクトーム：親電子性化合物によるタンパク質修飾の包括的解析

柴田貴広

第47回日本毒性学会学術年会シンポジウム  2020.7 

タンパク質化学修飾を基軸とした疾患マーカーの開発 Invited

柴田貴広

バイオインダストリー協会4大学医工連携セミナー  2021.2 

タンパク質との相互作用を介した食品成分の機能性

柴田貴広

バイオインダストリー協会機能性食品研究会第3回研究会  2024.1 

香辛料Grains of Paradise成分の抗肥満活性と作用メカニズムの解明

服部浩之、柴田貴広、光永徹

第72回日本木材学会  2022.3 

Pyrroloquinoline quinoneにより活性化されるGタンパク質共役型受容体の同定

6. 金森　星、若林万由香、下村沙也子、服部浩之、赤川　貢、井上飛鳥、内田浩二、柴田貴広

日本農芸化学会2022年度大会  2022.3 

アントシアニジンによる代謝物感受性受容体GPR35の活性化

下村沙也子、服部浩之、若林万由香、DIDIK HUSWO Utomo、北　将樹、井上飛鳥、内田浩二、柴田貴広

日本農芸化学会2022年度大会  2022.3 

アスコルビン酸酸化に起因するタンパク質ピロール化

吉武淳、柴田貴広、近澤未歩、内田浩二

日本農芸化学会2022年度大会  2022.3 

低密度リポタンパク質における酸化リン脂質修飾構造の網羅的解析

大塚優人、青山拓生、内田浩二、柴田貴広

第94回日本生化学会大会  2021.11 

アントシアニジンにより活性化されるGタンパク質共役型受容体の同定

下村沙也子、服部浩之、若林万由香、井上飛鳥、内田浩二、柴田貴広

日本ポリフェノール学会第14回学術集会  2021.9 

抗炎症性プロスタグランジンは血清存在下において炎症促進に作用する

柴田貴広, 山口悟, 森下望美, 河合慶親, 柴田亮行, 内田浩二

第８５回日本生化学会大会 

LOX-1リガンドであるケトアミド型リジン付加体のLC-MS/MS解析

柴田貴広、下津祐樹、町田幸子、加藤里奈、板部洋之、Xiaochun Zhu、Lawrence M. Sayre、内田浩二

第８４回日本生化学会大会 

*A monoclonal antibody against 4-ketoamide-type 4-oxo-2-nonenal-lysine adduct International conference

The 5th Joint Meeting ofThe Societies For Free Radical Research Australasia and Japan 

*イソチオシアネートによるレドックス制御を介した神経細胞分化促進機構の解析

第61回日本酸化ストレス学会学術集会 

新規脂質過酸化物4-オキソ-2-ノネナールの化学と生物

柴田貴広、内田浩二

第29回日本フリーラジカル学会/日本過酸化脂質・フリーラジカル学会第31回大会　合同学会 

タンパク質分子上に形成されるアダクトーム解析法の確立

2013.10 - 2017.3

さきがけ 

(財)旗影会　平成２４年度研究助成金

2012.6 - 2013.3

(財)加藤記念バイオサイエンス研究振興財団　　奨励研究助成

2009.4 - 2011.3

（財）飯島記念食品科学振興財団　学術研究助成

2009.4 - 2011.3

ネスレ栄養科学会議研究助成　平成19年度

2007.6

Diversity of oxidized lipids and their functional understanding

Grant number：23H05481  2023.4 - 2028.3

Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

Comprehensive understanding of oxidized lipids involved in disease induction

Grant number：20H00493  2020.4 - 2024.3

Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

疫病菌交配ホルモンが制御する性分化の分子機構の全容解明とその応用

Grant number：2818H03943  2018.4 - 2023.3

科学研究費補助金  基盤（A）

Elucidation and application of molecular mechanism of Phytophthora sexual reproduction controlled by mating hormones

Grant number：18H03943  2018.4 - 2023.3

Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

Analysis on molecular nutritional functions of bile acids as a feeding signal, and regulation of metabolic response to feeding by food factors

Grant number：15H05781  2015.5 - 2020.3

Sato Ryuichiro

摂食シグナル胆汁酸の分子栄養学的機能解析と食品成分による摂食応答制御

2015 - 2020.3

科学研究費補助金  基盤研究(S)

佐藤隆一郎

Formation and regulation of ligand signals associated with accepting and activating molecular oxygen

Grant number：26111011  2014.7 - 2019.3

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

UCHIDA Koji

脂質由来リガンドによる細胞応答と食品成分による制御

2012.4 - 2015.3

科学研究費補助金  基盤研究(C)

活性酸素リガンド・レセプター反応制御のケミカルバイオロジー

2008.4 - 2013.3

科学研究費補助金  新学術領域研究

食品由来の神経分化促進因子の探索

2007.4 - 2009.3

科学研究費補助金  若手研究(B)

柴田　貴広

プロスタグランジンなどの脂質メディエーターに関する研究

2003.4 - 2006.3

科学研究費補助金