Updated on 2024/02/09
Doctor of Science （ 1997.3 The University of Tokyo ）
Cell biology
Cell physiology
Space biology
Channel
Signal transduction
Mitochondria
Mechano-biology
Live imaging
Others / Others / Cell Biology
Others / Others / Biophysics
筋萎縮の病態に迫るミトコンドリアのメカノバイオロジー
名古屋大学大学院医学系研究科・講師
2015.6
Country：Japan
名古屋大学大学院医学系研究科・助教
2010.4 - 2015.5
Country：Japan
名古屋大学大学院医学系研究科・研究員
2005.4 - 2010.3
Country：Japan
JST/ERATO-SORST楠見プロジェクト・技術参事/研究員
1998.10 - 2005.3
Country：Japan
米NCI/NIH・協力研究員
1992.10 - 1994.9
花王株式会社
1990.3 - 1998.9
Country：Japan
The University of Tokyo Graduate School, Division of Science Zoological Science
1988.4 - 1990.3
Country： Japan
The University of Tokyo Faculty of Science Department of Biological Sciences
1984.4 - 1988.3
Country： Japan
the Biophysical Society of Japan
the Japanese Biochemical Society
the Molecular Biology Society of Japan
Japanese Society for Cell Biology
Physiological Society of Japan
Japanese Society for Pigment Cell Research
日本宇宙生物科学会
Muscle atrophy and mitochondrial Ca2+ levels in model organisms Invited
Precision Medicine Vol. 6 ( 8 ) page： 65 - 68 2023.7
Increased mitochondrial Ca2+ contributes to health decline with age and Duchene muscular dystrophy in C. elegans Reviewed International coauthorship
Higashitani A, Teranishi M, Nakagawa Y, Itoh Y, Sudevan S, Szewczyk NJ, Kubota Y, Abe T, Kobayashi T
FASEB J Vol. 37 page： e22851 2023.3
ミトコンドリアにおける機械刺激受容 Invited
小林 剛、田中瑞奈、寺西美佳、東谷篤志
細胞 Vol. 55 ( 2 ) page： 55 - 58 2023.2
Meclozine ameliorates skeletal muscle pathology and increases muscle forces in mdx mice Reviewed
Kawamura Yusuke, Hida Tetsuro, Ohkawara Bisei, Matsushita Masaki, Kobayashi Takeshi, Ishizuka Shinya, Hiraiwa Hideki, Tanaka Satoshi, Tsushima Mikito, Nakashima Hiroaki, Ito Kenyu, Imagama Shiro, Ito Mikako, Masuda Akio, Ishiguro Naoki, Ohno Kinji
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Vol. 592 page： 87 - 92 2022.2
ミトコンドリアにおけるメカニカルストレス感知と応答 Invited
小林 剛、田中瑞奈、寺西美佳、東谷篤志
細胞 Vol. 53 ( 12 ) page： 62 - 65 2021.10
ミトコンドリアに注目したメカノバイオロジー
小林 剛、田中瑞奈、寺西美佳、東谷篤志
Precision Medicine Vol. 4 ( 9 ) page： 873 - 877 2021.8
Sphingosine-1-phosphate Induces ATP Release via Volume-regulated Anion Channels in Breast Cell Lines Reviewed
Kishio Furuya, Hiroaki Hirata, Takeshi Kobayashi, Masahiro Sokabe
Vol. 11 ( 8 ) page： 851 2021.8
Morin attenuates dexamethasone-mediated oxidative stress and atrophy in mouse C2C12 skeletal myotubes Reviewed
Ulla Anayt, Uchida Takayuki, Miki Yukari, Sugiura Kosuke, Higashitani Atsushi, Kobayashi Takeshi, Ohno Ayako, Nakao Reiko, Hirasaka Katsuya, Sakakibara Iori, Nikawa Takeshi
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS Vol. 704 page： 108873 2021.6
Gravity sensing in plant and animal cells Reviewed
Vol. 7 ( 1 ) 2021.2
モデル生物を用いた筋の老化とミトコンドリア Invited
寺西美佳、小林 剛
細胞 Vol. 52 ( 12 ) page： 4 - 7 2020.10
ミトコンドリアはメカノセンサーか？－新規メカニカルストレス感知機構の解析 Invited
小林 剛、田中瑞奈、寺西美佳、東谷篤志
細胞 Vol. 52 ( 12 ) page： 51 - 54 2020.10
Ultra-high-purity iron is a novel and very compatible biomaterial Reviewed
Khan Luqman, Sato Katsumi, Okuyama Shinichi, Kobayashi Takeshi, Ohashi Kazumasa, Hirasaka Katsuya, Nikawa Takeshi, Takada Kunio, Higashitani Atsushi, Abiko Kenji
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS Vol. 106 page： 103744 2020.6
Reactive oxygen species upregulate expression of muscle atrophy-associated ubiquitin ligase Cbl-b in rat L6 skeletal muscle cells
Uchida Takayuki, Sakashita Yoshihiro, Kitahata Kanako, Yamashita Yui, Tomida Chisato, Kimori Yuki, Komatsu Akio, Hirasaka Katsuya, Ohno Ayako, Nakao Reiko, Higashitani Atsushi, Higashihata Akira, Ishioka Noriaki, Shimazu Toru, Kobayashi Takeshi, Okumura Yuushi, Choi Inho, Oarada Nlotoko, Mills Edward M., Teshima-Kondo Shigetada, Takeda Shin'ichi, Tanaka Eiji, Tanaka Keiji, Sokabe Masahiro, Nikawa Takeshi
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY Vol. 314 ( 6 ) page： C721-C731 2018.6
Identification of Meflin as a Potential Marker for Mesenchymal Stromal Cells Reviewed
Maeda K, Enomoto A, Hara A, Asai N, Kobayashi T, Horinouchi A, Maruyama S, Ishikawa Y, Nishiyama T, Kiyoi H, Kato T, Ando K, Weng L, Mii S, Asai M, Mizutani Y, Watanabe O, Hirooka Y, Goto H, Takahashi M
Sci Rep Vol. 6 2016
Ito G, Kobayashi T, Takeda Y, Sokabe M
Biochem Biophys Res Commun Vol. 456 ( 3 ) page： 792-8 2015
Cooperation of p40phox with p47phox for Nox2-based NADPH Oxidase Activation during Fcg Receptor (FcgR)-mediated Phagocytosis Reviewed
Ueyama T, Nakakita J, Nakamura T, Kobayashi T, Kobayashi T, Son J, Sakuma M, Sakaguchi H, Leto TL, Saito N
J Biol Chem Vol. 286 ( 47 ) page： 40693-40705 2011
Sensing substrate rigidity by mechanosensitive ion channels with stress fibers and focal adhesions. Reviewed
Kobayashi T, Sokabe M
Curr Opin Cell Biol Vol. 22 page： 669-676 2010
Stem cell factor/KIT signaling plays a pivotal role in regulating pigmentation in mammalian hair. Reviewed
Hachiya A, Sriwiriyanont P, Kobayashi T, Nagasawa A, Yoshida H, Ohuchi A, Kitahara T, Visscher MO, Takema Y, Tsuboi R, Boissy RE
J Pathol Vol. 218 page： 30-39 2009
Enzymological analysis of mutant protein kinase C gamma causing spinocerebellar ataxia type 14 and dysfunction in Ca2+ homeostasis. Reviewed
Adachi N, Kobayashi T, Takahashi H, Kawasaki T, Shirai Y, Ueyama T, Matsuda T, Seki T, Sakai N, Saito N
J Biol Chem Vol. 283 page： 19854-19863 2008
Regulation of clathrin dependent endocytosis by diacylglycerol kinase delta-importance of kinase activity and binding to AP2-alpha. Reviewed
Kawasaki T, Kobayashi T, Ueyama T, ShiraiY, SaitoN
Biochem J Vol. 409 page： 471-479 2008
Direct interaction of tyrosinase with Tyrp1 to form heterodimeric complexes in vivo. Reviewed
Kobayashi T, Hearing VJ
J Cell Sci Vol. 120 page： 4261-4268 2007
Membrane dynamics and cell signaling as studied by single molecule imaging.
Vol. 76 ( 2 ) page： 91-100 2004.2
Epitope mapping of the melanosomal matrix protein gp100 (PMEL17): Rapid processing in the endoplasmic reticulum and glycosylation in the early Golgi compartment. Reviewed
Yasumoto K, Watabe H, Valencia JC, Kushimoto T, Kobayashi T, Appella E, Hearing VJ
J Biol Chem Vol. 279 page： 28330-28338 2004
Mechanisms underlying the dysfunction of melanocytes in vitiligo epidermis: role of SCF/KIT protein interactions and the downstream effector, MITF-M. Reviewed
Kitamura R, Tsukamoto K, Harada K, Shimizu A, Shimada S, Kobayashi T, Imokawa G
J Pathol Vol. 202 page： 463-475 2004
Single-molecule imaging analysis of Ras activation in living cells. Reviewed
Murakoshi H, Iino R, Kobayashi T, Fujiwara T, Ohshima C, Yoshimura A, Kusumi A
Proc Natl Acad Sci USA Vol. 101 page： 7317-7322 2004
Central-pair-linked regulation of microtubule sliding by calcium in flagellar axonemes. Reviewed
Nakano I, Kobayashi T, Yoshimura M, Shingyoji C
J Cell Sci Vol. 116 page： 1627-1636 2003
Biochemical characterization of endothelin-converting enzyme-1alpha in cultured skin-derived cells and its postulated role in the stimulation of melanogenesis in human epidermis. Reviewed
Hachiya A, Kobayashi T, Takema Y, Imokawa G
J Biol Chem Vol. 277 page： 5395-5403 2002
メカノバイオロジー・再生工学におけるメカノバイオロジーII：創傷治癒／基質硬度検知／基質工学
木戸秋悟, 小林 剛（ Role： Joint author）
化学同人 2015
Ion channels activated by mechanical forces in bacterial and eukaryotic cells. (High Pressure Bioscience, Basic Concepts, Applications and Frontiers)
Sokabe M, Sawada M, Kobayashi T（ Role： Joint author）
Springer 2015 （ ISBN:978-94-017-9918-8 ）
ベーシックマスター細胞生物学・第５章「細胞膜」
小林 剛（ Role： Joint author）
オーム社(神谷 律、尾張部克志 編集) 2009
組織細胞化学2008・全反射蛍光顕微鏡(TIRFM)を用いたイメージング
小林 剛（ Role： Joint author）
日本組織細胞化学会 2008
染色・バイオイメージング実験ハンドブック・8章4「全反射蛍光観察法」
小林 剛（ Role： Joint author）
羊土社(高田邦昭、斎藤尚亮、川上速人編集) 2006
Mechano-sensing of mitochondria in cultured animal cells International conference
Kobayashi T, Tanaka M, Teranishi M, Nikawa T, Higashitani A
International Symposium on Mechanobiology for Human Health 2023.3.22 AMED
Towards understanding the gravity sensing in single cells Invited
2021.6.26
Cell Gravisensing Project International conference
2021.6.23
Substrate rigidity sensing of mesenchymal stem cells by utilizing Trpv4 channels Invited
Kobayashi T
2020.12.4
線虫を用いた筋ミトコンドリア研究 Invited
東谷篤志、小林 剛
第41回日本分子生物学会年会 ワークショップ3PW-09 「筋ミトコンドリア研究の新展開」 2018.11.30 日本分子生物学会
生きた細胞の中で反応と分子を見る Invited
小林 剛
徳島大学徳島大学大学院医歯薬学研究部 特別講義 2018.11.9 徳島大学徳島大学大学院医歯薬学研究部
Initial Step of Gravi-Sensing in Mesenchymal Stem Cells International conference
Kobayashi T, Sokabe M
the 13th Korea -Japan Joint Seminar on Space Environment Utilization Research 2016.10.25 JAXA/KARI
Possible Mechanisms for Gravity Sensing in Single Cells. Invited International conference
Sokabe M, Kobayashi T
11th Asian Microgravity Symposium (AMS2016)
Loss of Rigidity Sensing of Mesenchymal Stem Cells under Microgravity International conference
Kobayashi T, Tanaka M, Hashizume T, Higashibata A, Yano S, Nikawa T, Sokabe M
11th Asian Microgravity Symposium (AMS2016)
動物細胞における微小重力環境センシング機構 Invited
小林 剛、曽我部正博
日本宇宙生物科学会第29回大会 シンポジウム5「重力感知のメカノバイオロジー」 2015.9.26 日本宇宙生物学会
細胞メカニクス・システム：アクティブタッチによる基質の硬さ感知 Invited
小林 剛、曽我部正博
第63回高分子討論会 特定テーマS18「生物模倣による新規機能性材料・次世代型プロセスの創成」 日本高分子学会・バイオミメティク研究会
Cellular Substrate Rigidity Sensing: Possible Role of Mechanosensitive Ion Channels with Stress Fibers and Focal Adhesions
Kobayashi T, Sokabe M
International Symposium on Innovative Nanobiodevices 2012 (ISIN 2012)
Sensing Substrate Rigidity by Mechanosensitive Ion Channels with Stress Fibers and Focal Adhesions International conference
Kobayashi T, Sokabe M
International Symposium on Mechanobiology
Single molecule imaging of signalling molecules and ion channels in living cells. International conference
The 36th International Congress of Physiological Science, Satellite Symposium #8
Clathrin-independent pathway for internalizing c-kit that remains active in endocytic vesicles. International conference
19th International Pigment Cell Conference, Concurrent session 4: Innovative Technology
1分子FRETで見た生細胞におけるRasシグナル伝達
小林 剛、楠見明弘
第82回日本生理学会大会シンポジウム6 「FRET で探る細胞内シグナル伝達と分子構造変」
細胞膜上で一時的に形成される信号複合体におけるRasシグナル伝達
小林 剛、村上瑞奈、武田美江、吉村昭彦、楠見明弘
第27回日本分子生物学会年会ワークショップW1N「シグナル伝達分子のダイナミクス」
活性化したc-Kitのクラスリン非依存的な細胞内への取り込み機構
小林 剛、武田美江、村上瑞奈、藤原敬宏、吉村昭彦、楠見明弘
第77回日本生化学会大会ワークショップW28「生体膜のダイナミズム」
Single molecule analysis of Ras-Raf signaling in living cells. International conference
12th International Congress of Histochemistry and Cytochemistry, Symposium: Visualizing of signal transduction
筋萎縮の病態に迫るミトコンドリアのメカノバイオロジー
2018.11 - 2022.3
革新的先端研究開発支援事業・メカノバイオロジー機構の解明による革新的医療機器及び医療技術の創出
Grant type：Competitive
Imaging analysis of ER-mitochondria contact sites in living cells
Grant number：21K06149 2021.4 - 2024.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Authorship：Principal investigator
Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）
Analysis of mechanosensing/transduction in costamere by ultrasound
Grant number：21K11274 2021.4 - 2024.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Authorship：Coinvestigator(s)
Mechano-nutrition-signaling for disuse muscle atrophy
Grant number：19H04054 2019.4 - 2022.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Authorship：Coinvestigator(s) Grant type：Competitive
Imaging analysis of mechno-sensing in mitochondria of living cells
Grant number：18K19291 2018.6 - 2021.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
KOBAYASHI Takeshi
Authorship：Principal investigator
Grant amount：\6370000 （ Direct Cost: \4900000 、 Indirect Cost：\1470000 ）
Animal cell utilizes mechanosensors that sense and convert mechanical signals into biochemical signals. However, the details of those sensors or signals remain unclear. Recently mitochondria have been demonstrated to activate several intracellular signaling pathways upon mechanical stress. Therefore, we hypothesize that mitochondria can feel and respond to mechanical stress as mechanosensors. In this project, we try to verify our hypothesis and to investigate the mitochondrial signaling pathways upon mechanical stress. We found that cultured mesenchymal stem cells elevated mitochondrial calcium concentration in response to mechanical stress, resulting in increased mitochondrial ATP levels. Pharmacological studies showed that the activity of mechanosensitive channels activities was not essential to the changes in mitochondrial calcium concentration. These results support our hypothesis that mitochondria could sense mechanical stimuli and transduce them to biochemical signals.
Mechanosensing Mechanisms for Sensing Mechanical Stress Including Gravitational Changes
Grant number：15H05936 2015.6 - 2020.3
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Naruse Keiji
Authorship：Coinvestigator(s)
This study developed a centrifugal fluorescence microscope system that can observe the gravity response of cells in real time. In this way, we succeeded in live imaging of morphological changes of cells due to gravitational stimulation by rotation for several tens of minutes.
We also discovered a new mechanism for the gravity sensing of cells. In space, the bone density of astronauts decreases. In agreement with this, simulated microgravity acts as a suppressive factor in bone differentiation of mesenchymal stem cells. Our results suggest that the activity of the mechanosensitive ion channel TRPV4 is reduced in the simulated microgravity environment, and the activity of the protein kinase C is subsequently reduced to suppress the nuclear translocation of the transcriptional cofactor YAP. In addition, we have also developed a statistical method for uniformly analyzing gene expression levels among different species.
培養動物細胞における重力感知・応答機構の解析
2014.4 - 2017.3
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
生物規範メカニクス・システム
2012.4 - 2017.3
科学研究費補助金 新学術領域研究（研究領域提案型）
Authorship：Coinvestigator(s)
細胞内１分子計測法を用いた機械刺激によるＳｒｃキナーゼ活性化機構の解析
2011.4 - 2014.3
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
血流センサーＰ２Ｘ４受容体のダイナミクス制御のイメージング解析
2008.4 - 2011.3
科学研究費補助金 基盤研究(C)
小林 剛
Authorship：Principal investigator
細胞内１分子計測法を用いた機械刺激受容センサーの作動機構の解明
2006.4 - 2008.3
科学研究費補助金 基盤研究(C)
小林 剛
Authorship：Principal investigator
生体の機能I
2022
基礎セミナーB
2022
基礎セミナーB
2021
基礎医学セミナー
2021
生体の機能I
2021
基礎医学セミナー
2020
基礎セミナーB
2020
Physiology I
2020
