研究者詳細 - 荘司　長三

論文 - 荘司　長三

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Enhanced cis- and enantioselective cyclopropanation of styrene catalysed by cytochrome P450BM3 using decoy molecules 査読有り

Suzuki Kazuto, Shisaka Yuma, Stanfield Joshua Kyle, Watanabe Yoshihito, Shoji Osami

CHEMICAL COMMUNICATIONS 56 巻 ( 75 ) 頁： 11026 - 11029 2020年9月

Systematic Evolution of Decoy Molecules for the Highly Efficient Hydroxylation of Benzene and Small Alkanes Catalyzed by Wild-Type Cytochrome P450BM3 査読有り

Yonemura Kai, Ariyasu Shinya, Stanfield Joshua Kyle, Suzuki Kazuto, Onoda Hiroki, Kasai Chie, Sugimoto Hiroshi, Aiba Yuichiro, Watanabe Yoshihito, Shoji Osami

ACS CATALYSIS 10 巻 ( 16 ) 頁： 9136 - 9144 2020年8月

Control of microenvironment around enzymes by hydrogels 査読有り

Kobayashi Yuichiro, Kohara Kenji, Kiuchi Yusuke, Onoda Hiroki, Shoji Osami, Yamaguchi Hiroyasu

CHEMICAL COMMUNICATIONS 56 巻 ( 49 ) 頁： 6723 - 6726 2020年6月

Crystals in Minutes: Instant On-Site Microcrystallisation of Various Flavours of the CYP102A1 (P450BM3) Haem Domain 査読有り

Stanfield Joshua Kyle, Omura Keita, Matsumoto Ayaka, Kasai Chie, Sugimoto Hiroshi, Shiro Yoshitsugu, Watanabe Yoshihito, Shoji Osami

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 59 巻 ( 19 ) 頁： 7611 - 7618 2020年5月

Kristalle in Minutenschnelle: Sofortige Mikrokristallisation verschiedenster Varianten der CYP102A1‐(P450BM3)‐Hämdomäne

Joshua Kyle Stanfield, Keita Omura, Ayaka Matsumoto, Chie Kasai, Hiroshi Sugimoto, Yoshitsugu Shiro, Yoshihito Watanabe, Osami Shoji

Angewandte Chemie 132 巻 ( 19 ) 頁： 7681 - 7689 2020年5月

Cationic guanine: positively charged nucleobase with improved DNA affinity inhibits self-duplex formation 査読有り

Hibino Masaki, Aiba Yuichiro, Shoji Osami

CHEMICAL COMMUNICATIONS 56 巻 ( 17 ) 頁： 2546 - 2549 2020年2月

Development of a High-Pressure Reactor Based on Liquid-Flow Pressurisation to Facilitate Enzymatic Hydroxylation of Gaseous Alkanes 査読有り

Ariyasu Shinya, Kodama Yusaku, Kasai Chie, Cong Zhiqi, Stanfield Joshua Kyle, Aiba Yuichiro, Watanabe Yoshihito, Shoji Osami

CHEMCATCHEM 11 巻 ( 19 ) 頁： 4709 - 4714 2019年10月

Hijacking the Heme Acquisition System of Pseudomonas aeruginosa for the Delivery of Phthalocyanine as an Antimicrobial 査読有り

Shisaka Yuma, Iwai Yusuke, Yamada Shiho, Uehara Hiromu, Tosha Takehiko, Sugimoto Hiroshi, Shiro Yoshitsugu, Stanfield Joshua K., Ogawa Kazuya, Watanabe Yoshihito, Shoji Osami

ACS CHEMICAL BIOLOGY 14 巻 ( 7 ) 頁： 1637 - 1642 2019年7月

Highly malleable haem-binding site of the haemoprotein HasA permits stable accommodation of bulky tetraphenylporphycenes 査読有り

Sakakibara Erika, Shisaka Yuma, Onoda Hiroki, Koga Daiki, Xu Ning, Ono Toshikazu, Hisaeda Yoshio, Sugimoto Hiroshi, Shiro Yoshitsugu, Watanabe Yoshihito, Shoji Osami

RSC ADVANCES 9 巻 ( 32 ) 頁： 18697 - 18702 2019年6月

The effect of decoy molecules on the activity of the P450Bm3 holoenzyme and a heme domain peroxygenase variant 査読有り

Dezvarei Shaghayegh, Shoji Osami, Watanabe Yoshihito, Bell Stephen G.

CATALYSIS COMMUNICATIONS 124 巻頁： 97 - 102 2019年5月

Hoodwinking Cytochrome P450BM3 into Hydroxylating Non-Native Substrates by Exploiting Its Substrate Misrecognition 査読有り

Shoji Osami, Aiba Yuichiro, Watanabe Yoshihito

ACCOUNTS OF CHEMICAL RESEARCH 52 巻 ( 4 ) 頁： 925 - 934 2019年4月

Frontispiece: Whole-Cell Biotransformation of Benzene to Phenol Catalysed by Intracellular Cytochrome P450BM3 Activated by External Additives 査読有り

Masayuki Karasawa, Joshua Kyle Stanfield, Sota Yanagisawa, Osami Shoji, Yoshihito Watanabe

Angewandte Chemie International Edition 57 巻 ( 38 ) 頁： 12264-12269 2018年9月

Whole-Cell Biotransformation of Benzene to Phenol Catalysed by Intracellular Cytochrome P450BM3 Activated by External Additives

Karasawa Masayuki, Stanfield Joshua Kyle, Yanagisawa Sota, Shoji Osami, Watanabe Yoshihito

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 57 巻 ( 38 ) 頁： 12264 - 12269 2018年9月

Frontispiz: Ganzzellbiotransformation von Benzol zu Phenol durch intrazelluläres Zytochrom P450BM3 aktiviert mithilfe externer Zusätze

Masayuki Karasawa, Joshua Kyle Stanfield, Sota Yanagisawa, Osami Shoji, Yoshihito Watanabe

Angewandte Chemie 130 巻 ( 38 ) 頁： 12444 - 12449 2018年9月

Whole-Cell Biotransformation of Benzene to Phenol Catalysed by Intracellular Cytochrome P450BM3 Activated by External Additives 査読有り

M. Karasawa, J. K. Stanfield, S. Yanagisawa, O. Shoji, Y. Watanabe

Angew. Chem. Int. Ed. 57 巻頁： 12264-12269 2018年9月

Peptide Nucleic Acid Conjugated with Ruthenium-Complex Stabilizing Double-Duplex Invasion Complex Even under Physiological Conditions 査読有り

Masaki Hibino, Yuichiro Aiba, Yoshihito Watanabe, Osami Shoji

ChemBioChem 19 巻 ( 15 ) 頁： 1601-1604 2018年8月

Metabolic enhancement of 2,3´,4,4´,5-pentachlorobiphenyl (CB118) using cytochrome P450 monooxygenase isolated from soil bacterium under the presence of perfluorocarboxylic acids (PFCAs) and the structural basis of its metabolism 査読有り国際誌

E. Goto, Y. Haga, M. Kubo, T. Itoh, C. Kasai, O. Shoji, K. Yamamoto, C. Matsumura, T. Nakano, H. Inui

Chemosphere 210 巻頁： 376-383 - 383 2018年7月

Dual-Functional Small Molecules for Generating an Efficient Cytochrome P450BM3 Peroxygenase 査読有り

Nana Ma, Zhifeng Chen, Jie Chen, Jingfei Chen, Cong Wang, Haifeng Zhou, Lishan Yao, Osami Shoji, Yoshihito Watanabe, Zhiqi Cong

Angewandte Chemie - International Edition 57 巻 ( 26 ) 頁： 7628 - 7633 2018年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Wiley-VCH Verlag

We report a unique strategy for the development of a H2O2-dependent cytochrome P450BM3 system, which catalyzes the monooxygenation of non-native substrates with the assistance of dual-functional small molecules (DFSMs), such as N-(ω-imidazolyl fatty acyl)-l-amino acids. The acyl amino acid group of DFSM is responsible for bounding to enzyme as an anchoring group, while the imidazolyl group plays the role of general acid–base catalyst in the activation of H2O2. This system affords the best peroxygenase activity for the epoxidation of styrene, sulfoxidation of thioanisole, and hydroxylation of ethylbenzene among those P450–H2O2 system previously reported. This work provides the first example of the activation of the normally H2O2-inert P450s through the introduction of an exogenous small molecule. This approach improves the potential use of P450s in organic synthesis as it avoids the expensive consumption of the reduced nicotinamide cofactor NAD(P)H and its dependent electron transport system. This introduces a promising approach for exploiting enzyme activity and function based on direct chemical intervention in the catalytic process.

DOI： 10.1002/anie.201801592

Web of Science

Scopus

Hydroxylation of gaseous alkanes and benzene catalyzed by cytochrome P450BM3 using decoy molecules as a substrate analogue

Shoji Osami, Watanabe Yoshihito

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 255 巻頁： . 2018年3月

Switchable stereoselectivity in monooxygenation of non-native substrates by P450BM3 using decoy molecules

Suzuki Kazuto, Shoji Osami, Stanfield Joshua, Sugimoto Hiroshi, Shiro Yoshitsugu, Watanabe Yoshihito

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 255 巻頁： . 2018年3月

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