Updated on 2024/04/02
Ph.D. （ 1993.6 Fukui Medical University ）
Life Science / Applied biochemistry / Applied Biochemistry
Life Science / Applied molecular and cellular biology / Applied Molecular Cell Biology
Analysis of the control mechanisms of translation (protein synthesis) in the higher animals
Molecular mechanism of mammary gland involution
名古屋大学大学院生命農学研究科 准教授（職名変更）
2007.4
Country：Japan
"Nagoya University, Graduate School of Bioagricultural Sciences, Associate Professor"
2001.9 - 2007.3
Country：Japan
"RIKEN, Molecular Oncology Laboratory, Senior Research Scientist"
2000.4 - 2001.8
Country：Japan
"RIKEN, Molecular Oncology Laboratory, Contact Researcher"
1998.10 - 2000.3
Country：Japan
"the Burnham Institute, Research Fellow"
1996.4 - 1998.9
Country：United States
"Fukui Medical School, Department of Legal Medicine, Research Assistant"
1990.4 - 1996.4
Country：Japan
"National Institute of Police Science, Researcher"
1986.4 - 1990.3
Country：Japan
The University of Tokyo Graduate School, Division of Science Department of Biophysics and Biochemistry
- 1986
Country： Japan
The University of Tokyo Faculty of Science Department of Biophysics and Biochemistry
- 1984
Country： Japan
Japan Society for Bioscience, Biotechnology, and Agrochemistry
Molecular Biology Society of Japan
Japanese Biochemical Society
Japanese Cancer Association
Characterization of human Ccser2 as a protein tracking the plus-ends of microtubules.
Shirai Y, Okuda T, Oshima K, Nadano D
BMC research notes Vol. 16 ( 1 ) page： 198 2023.9
Hyaluronan degradation and release of a hyaluronan-aggrecan complex from perineuronal nets in the aged mouse brain
Sugitani Kei, Egorova Diana, Mizumoto Shuji, Nishio Shunsuke, Yamada Shuhei, Kitagawa Hiroshi, Oshima Kenzi, Nadano Daita, Matsuda Tsukasa, Miyata Shinji
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS Vol. 1865 ( 2 ) page： 129804 2021.2
A pericellular hyaluronan matrix is required for the morphological maturation of cortical neurons
Takechi Mina, Oshima Kenzi, Nadano Daita, Kitagawa Hiroshi, Matsuda Tsukasa, Miyata Shinji
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS Vol. 1864 ( 10 ) page： 129679 2020.10
Transcription Factor Sox4 as a Potential Player in Mammary Gland Involution
Shibayama Hirohisa, Yamamoto Tomomi, Oshima Kenzi, Matsuda Tsukasa, Nadano Daita
DNA AND CELL BIOLOGY Vol. 38 ( 10 ) page： 1125 - 1133 2019.10
Apical-to-basolateral transepithelial transport of cow's milk caseins by intestinal Caco-2 cell monolayers: MS-based quantitation of cellularly degraded alpha- and beta-casein fragments
Sakurai Nao, Nishio Shunsuke, Akiyama Yuka, Miyata Shinji, Oshima Kenzi, Nadano Daita, Matsuda Tsukasa
JOURNAL OF BIOCHEMISTRY Vol. 164 ( 2 ) page： 113-125 2018.8
The mRNA-binding protein Serbp1 as an auxiliary protein associated with mammalian cytoplasmic ribosomes
Muto Akiko, Sugihara Yoshihiko, Shibakawa Minami, Oshima Kenzi, Matsuda Tsukasa, Nadano Daita
CELL BIOCHEMISTRY AND FUNCTION Vol. 36 ( 6 ) page： 312-322 2018.8
Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells
Ooishi Takuya, Nadano Daita, Matsuda Tsukasa, Oshima Kenzi
GENES TO CELLS Vol. 22 ( 10 ) page： 885-899 2017.10
RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice
Jiang Long, Li Tao, Zhang Xingxia, Zhang Beibei, Yu Changping, Li Yang, Fan Suixing, Jiang Xiaohua, Khan Teka, Hao Qiaomei, Xu Peng, Nadano Daita, Huleihel Mahmoud, Lunenfeld Eitan, Wang P. Jeremy, Zhang Yuanwei, Shi Qinghua
CURRENT BIOLOGY Vol. 27 ( 10 ) page： 1498-+ 2017.5
Evaluation of allergenic potential for rice seed protein components utilizing a rice proteome database and an allergen database in combination with IgE-binding of recombinant proteins Reviewed
Hirano, K., Hino, S., Oshima, K., Nadano, D., Urisu, A., Takaiwa, F., and Matsuda, T.
Biosci. Biotechnol. Biochem. Vol. 80 ( 3 ) page： 564-573 2016.3
A protective effect of milk-fat globule EGF factor VIII (MFG-E8) on the spontaneous fusion of milk fat globules in breast milk. Reviewed
Yasueda, T., Oshima, K., Nakatani, H., Tabuchi, K., Nadano, D., and Matsuda, T.
J. Biochem. Vol. 158 ( 1 ) page： 25-35 2015.7
Impaired O-linked N-acetylglucosaminylation in the endoplasmic reticulum by mutated epidermal growth factor (EGF) domain-specific O-Linked N-acetylglucosamine transferase found in Adams-Oliver syndrome. Reviewed
Ogawa, M., Sawaguchi, S., Kawai, T., Nadano, D., Matsuda, T., Yagi, H., Kato, K., Furukawa, K., and Okajima, T.
J. Biol. Chem. Vol. 290 ( 4 ) page： 2137-2149 2015.1
Glycosylated Chicken ZP2 Accumulates in the Egg Coat of Immature Oocytes and Remains Localized to the Germinal Disc Region of Mature Eggs. Reviewed
Nishio, S., Kohno, Y., Iwata, Y., Arai, M., Okumura, H., Oshima, K., Nadano, D., and Matsuda, T.
Biol Reprod. Vol. 91 ( 5 ) page： 107, 1-10 2014.11
Lyar, a cell growth-regulating zinc finger protein, was identified to be associated with cytoplasmic ribosomes in male germ and cancer cells. Reviewed
Yonezawa, K., Sugihara, Y., Oshima, K., Matsuda, T., and Nadano, D.
Mol. Cell. Biochem. Vol. 395 ( 1-2 ) page： 221-229 2014.10
A lactoferrin-receptor, intelectin 1, affects uptake, sub-cellular localization and release of immunochemically detectable lactoferrin by intestinal epithelial Caco-2 cells. Reviewed
Akiyama Y, Oshima K, Kuhara T, Shin K, Abe F, Iwatsuki K, Nadano D, Matsuda T
Vol. 154 ( 2 ) page： 437-448 2013.11
Allergenic potential of rice-pollen proteins: expression, immuno-cross reactivity and IgE-binding. Reviewed
Hirano K, Hino S, Oshima K, Okajima T, Nadano D, Urisu A, Takaiwa F, Matsuda T
J Biochem. Vol. 154 ( 2 ) page： 195-205 2013.8
Identification and expression of an autosomal paralogue of ribosomal protein S4, X-linked, in mice: Potential involvement of testis-specific ribosomal proteins in translation and spermatogenesis. Reviewed
Sugihara, Y., Sadohara, E., Yonezawa, K., Kugo, M., Oshima, K., Matsuda, T., and Nadano, D.
Gene Vol. 521 ( 1 ) page： 91-99 2013.5
Intracellular retention and subsequent release of bovine milk lactoferrin taken up by human enterocyte-like cell lines, Caco-2, C2BBe1 and HT-29. Reviewed
Akiyama Y, Oshima K, Shin K, Wakabayashi H, Abe F, Nadano D, Matsuda T.
Biosci. Biotechnol. Biochem. Vol. 77 ( 5 ) page： 1023-1029 2013.5
Post-weaning increases in the milk-fat globule EGF-factor VIII on fat globules in mouse milk and in the uptake of the fat globules by HC11 mammary epithelial cells. Reviewed
Nakatani H, Yasueda T, Oshima K, Okajima T, Nadano D, Flint DJ, Matsuda T.
Vol. 153 ( 1 ) page： 31-41 2013.1
Enhancement of mouse sperm motility by trophinin-binding peptide. Reviewed
Park SK, Yoon J, Wang L, Shibata TK, Motamedchaboki K, Shim KJ, Chang MS, Lee SH, Tamura N, Hatakeyama S, Nadano D, Sugihara K, Fukuda MN.
Reprod. Biol. Endocrinol. Vol. 10 ( 1 ) page： 101 2012.11
Re-evaluation of milk-fat globule EGF-Factor VIII (MFG-E8) as an intrinsic component on the mouse milk-fat globule membrane. Reviewed
Nakatani, H., Yasueda, T., Oshima, K., Nadano, D., Flint, D., and Matsuda T.
Biosci. Biotech. Biochem. Vol. 76 ( 11 ) page： 2055-2060 2012.11
The murine Gcap14 gene encodes a novel microtubule binding and bundling protein. Reviewed
Hosono, H., Yamaguchi, N., Oshima, K., Matsuda, T., and Nadano, D.
FEBS Lett. Vol. 586 ( 10 ) page： 1426-1430 2012.5
Discharge of solubilized and Dectin-1-reactive β-glucan from macrophage cells phagocytizing insoluble β-glucan particles: involvement of reactive oxygen species (ROS)-driven degradation. Reviewed
Hino, S., Kito, A., Yokoshima, R., Sugino, R., Oshima, K., Morita, T., Okajima, O., Nadano, D., Uchida, K. and Matsuda, T.
Biochem. Biophys. Res. Commun. Vol. 421 ( 2 ) page： 329-334 2012.5
O-linked-N-acetylglucosamine modification of mammalian Notch receptors by an atypical O-GlcNAc transferase Eogt1. Reviewed
Sakaidani, Y., Ichiyanagi, N., Saito, C., Nomura, T., Ito, M., Nishio, Y., Nadano, D., Matsuda, T., Furukawa, K., and Okajima, T.
Biochem. Biophys. Res. Commun. Vol. 419 ( 1 ) page： 14-19 2012.3
O-Linked-N-acetylglucosamine on extracellular protein domains mediates epithelial cell-matrix interactions. Reviewed
Sakaidani, Y., Nomura, T., Matsuura, A., Ito, M., Suzuki, E., Murakami, K., Nadano, D., Matsuda, T., Furukawa, K., and Okajima, T.
Nat. Commun. Vol. 2 page： 583 2011.12
Secretion of three enzymes for fatty acid synthesis into mouse milk in association with fat globules, and rapid decrease of the secreted enzymes by treatment with rapamycin. Reviewed
Moriya, H., Uchida, K., Okajima, T., Matsuda, T., and Nadano, D.
Arch. Biochem. Biophys. Vol. 508 ( 1 ) page： 87-92 2011.4
Transplantation of a mammary stromal cell line into a mammary fat pad: development of the site-specific in vivo analysis system for mammary stromal cells. Reviewed
Nakatani, H., Aoki, N., Nadano, D., and Matsuda T.
Biosci. Biotechnol. Biochem. Vol. 75 ( 3 ) page： 550-555 2011.3
IgG2 dominancy and carbohydrate recognition specificity of C3H/He mouse antibodies directed to cross-reactive carbohydrate determinants (CCDs) bearing ß-(1,2)-xylose and α-(1,3)-fucose. Reviewed
Hino, S., Umeda, F., Inumaru, S., Aoki, N. Sato, C., Okajima, T., Nadano, D., and Matsuda, T.
Immunol Lett. Vol. 133 ( 1 ) page： 28-34 2010.9
Identification of heterogeneous nuclear ribonucleoprotein A/B as a cytoplasmic mRNA-binding protein in early involution of the mouse mammary gland. Reviewed
Taga, Y., Miyoshi, M., Okajima, T., Matsuda, T., and Nadano, D.
Cell Biochem. Function Vol. 28 ( 4 ) page： 321-328 2010.6
Proteomic analysis of rodent ribosomes revealed heterogeneity including ribosomal proteins L10-like, L22-like 1, and L39-like. Reviewed
Sugihara, Y., Honda, H., Iida, T., Morinaga, T., Hino, S., Okajima, T., Matsuda, T., and Nadano, D.
J. Proteome Res. Vol. 9 ( 3 ) page： 1351-1366 2010.3
Binding of Norovirus virus-like particles (VLPs) to human intestinal Caco-2 cells and suppressive effect of pasteurized bovine colostrum on the VLPs-binding. Reviewed
Murakami, K., Aoki, N., Nakatani, H., Asano, Y., Miura, Y., Okajima, T., Nadano, D., Inagaki, M., Kanamaru, Y., and Matsuda, T.
Biosci. Biotech. Biochem. Vol. 74 ( 3 ) page： 541-547 2010.3
Establishment of a mammary stromal fibroblastic cell line for in vitro studies in mice of mammary adipocyte differentiation. Reviewed
Nakatani, H., Aoki, N., Okajima, T., Nadano, D., Flint, D., and Matsuda T.
Biol. Reprod. Vol. 82 ( 1 ) page： 44-53 2010.1
Serum and monoclonal IgE antibodies from NC/Nga mice with severe atopic-like dermatitis recognize an autoantigen, histone H3. Reviewed
Matsubara, T., Aoki, N., Hino, S., Okajima, T., Nadano, D., and Matsuda, T.
Clin. Exp. Allergy Vol. 39 ( 4 ) page： 579-590 2009.4
Periodate-resistant carbohydrate epitopes recognized by IgG and IgE antibodies from some of the immunized mice and patients with allergy. Reviewed
Hino, S., Matsubara, T., Urisu, A., Aoki, N., Sato, C., Okajima, T., Nadano, D., and Matsuda, T.
Biochem. Biophys. Res. Commun. Vol. 380 ( 3 ) page： 632-637 2009.3
Development and evaluation of transgenic rice seeds accumulating a type II-collagen tolerogenic peptide. Reviewed
Hashizume, F., Hino, S., Kakehashi, M., Okajima, O., Nadano, D., Aoki, N., and Matsuda, T.
Transgenic Res. Vol. 17 ( 6 ) page： 1117-1129 2008.12
O-linked GlcNAc is present on the extracellular domain of notch receptors. Reviewed
Matsuura, A., Ito, M., Sakaidani, Y., Kondo, T., Murakami, K., Furukawa, K, Nadano, D. , Matsuda, T., and Okajima, T.
J. Biol. Chem. Vol. 283 ( 51 ) page： 35486-35495 2008.12
Absorption, migration and kinetics in peripheral blood of orally administered ovalbumin in a mouse model. Reviewed
Matsubara, T., Aoki, N., Honjoh, T., Mizumachi, K., Kurahashi, J., Okajima, T., Nadano, D., and Matsuda, T.
Biosci. Biotech. Biochem. Vol. 72 ( 10 ) page： 2555-2565 2008.10
Suppressive expression of the glutelin A1-collagen peptide fusion gene in hybrid rice lines with the mutant line, Low Glutelin Content-1 (LGC-1). Reviewed
Hashizume, F., Hino, S., Okajima, T., Nadano, D., Aoki, N., and Matsuda, T.
Plant Biotechnol. Vol. 25 ( 5 ) page： 465-471 2008.9
Enhancement of human sperm motility by trophinin-binding peptide. Reviewed
Hatakeyama, S., Sugihara, K., Lee, S. H., Nadano, D., Nakayama, J. Ohyama, C., and Fukuda, M. N.
J. Urol. Vol. 180 ( 2 ) page： 767-771 2008.8
The role of bystin in embryo implantation and in ribosomal biogenesis. Invited Reviewed
Fukuda, M. N., Miyoshi, M., and Nadano, D.
Cell. Mol. Life Sci. Vol. 65 ( 1 ) page： 92-99 2008.1
Association of chicken zona pellucida glycoprotein (ZP) B1 with ZPC induces formation of ZPB1-ZPC fibrous aggregates containing disulfide-bridged ZPB1 dimer. Reviewed
Okumura, H., Okajima, T., Nadano, D., and Matsuda, T.
Vol. 364 ( 3 ) page： 682-688 2007.12
The role of trophinin, an adhesion molecule unique to human trophoblasts, in progression of colorectal cancer. Reviewed
Harada, O., Suga, T., Suzuki, T., Nakamoto, K., Kobayashi, M., Nomiyama, T., Nadano, D. , Ohyama, C., Fukuda, M. N., and Nakayama, J.
Int. J. Cancer Vol. 121 ( 5 ) page： 1072-1078 2007.9
Bystin in human cancer cells: intracellular localization and function in ribosome biogenesis. Reviewed
Miyoshi, M., Okajima, T., Matsuda, T., Fukuda, M. N., and Nadano, D.
Biochem. J. Vol. 404 ( 3 ) page： 373-381 2007.6
Trophoblast cell activation by trophinin ligation is implicated in human embryo implantation. Reviewed
Sugihara, K., Sugiyama, D., Byrne, J., Wolf, D. P., Lowitz, K. P., Kobayashi, Y., Kabir-Salmani, M., Nadano, D., Aoki, D., Nozawa, S., Nakayama, J., Mustelin, T., Ruoslahti, E., Yamaguchi, N., and Fukuda, M. N.
Proc. Nat. Acad. Sci. USA Vol. 104 ( 10 ) page： 3799-3804 2007.3
Heterocomplex formation and cell-surface accumulation of hen's serum zona pellucida B1 (ZPB1)with ZPC expressed by a mammalian cell line (COS-7): a possible initiating step of egg-envelope matrix construction. Reviewed
Okumura, H., Aoki, N., Sato, C., Nadano, D., and Matsuda, T.
Biol. Reprod. Vol. 76 ( 1 ) page： 9-18 2007.1
The Bysl gene product, bystin, is essential for survival of mouse embryos. Reviewed
Aoki, R,, Suzuki, N., Paria, B. C., Sugihara, K., Akama, T. O., Raab, G., Miyoshi, M., Nadano, D., and Fukuda, M. N.
FEBS Lett. Vol. 580 ( 26 ) page： 6062-6068 2006.11
Antioxidant and antibacterial genes are upregulated in early involution of the mouse mammary gland: sharp increase of ceruloplasmin and lactoferrin in accumulating breast milk. Reviewed
Nakamura, M., Tomita, A., Nakatani, H, Matsuda, T., and Nadano, D.
DNA Cell Biol. Vol. 25 ( 9 ) page： 491-500 2006.9
Weaning-induced expression of a milk-fat globule protein, MFG-E8, in mouse mammary glands as demonstrated by the analyses of its mRNA, protein and phosphatidylserine-binding activity. Reviewed
Nakatani, H., Aoki, N., Nakagawa, Y., Jin-no, S., Aoyama, K., Oshima, K., Ohira, S., Sato, C., Nadano D., and Matsuda, T.
Biochem. J. Vol. 395 ( 1 ) page： 21-30 2006.4
Apical cell adhesion molecule, trophinin, localizes to cell surface, cytoplasm and nuclear envelope. Reviewed
Aoyama, J., Nakayama, Sugiyama, D., Nadano, D., Fukuda, M. N., and Yamaguchi, N.
FEBS Lett. Vol. 579 ( 28 ) page： 6326-6332 2005.11
A newly identified ZP glycoprotein, ZPD, and dimeric ZP1 of chicken egg-envelope are involved in sperm activation on sperm-egg interaction. Reviewed
Okumura, H., Kohno, Y., Iwata, Y., Mori, H., Aoki, N., Sato, C., Kitajima, K., Nadano, D., and Matsuda, T.
Biochem. J. Vol. 384 ( 1 ) page： 191-199 2004.11
Implantation-dependent expression of trophinin by maternal fallopian tube epithelia during tubal pregnancies: possible role of human chorionic gonadotrophin on ectopic pregnancy. Reviewed
Nakayama, J., Aoki, D., Suga, T., Akama, T. O., Ishizone, S., Yamaguchi, H., Imakawa, K., Nadano, D., Fazleabas, A. T., Katsuyama, T., Nozawa, S., and Fukuda, M. N.
Am. J. Pathol. Vol. 163 ( 6 ) page： 2211-2219 2003.12
Differential expression of ribosomal proteins in human normal and neoplastic colorectum. Reviewed
Kasai, H., Nadano, D., Hidaka, E., Higuchi, K., Kawakubo, M., Sato, T., and Nakayama, J.
J. Histochem. Cytochem. Vol. 51 ( 5 ) page： 567-574 2003.5
A human gene encoding a protein homologous to ribosomal protein L39 is normally expressed in the testis and derepressed in multiple cancer cells. Reviewed
Nadano, D., Notsu, T., Matsuda, T., and Sato, T.
Biochim. Biophys. Acta Vol. 1577 ( 3 ) page： 430-436 2002.9
Human tastin, a proline-rich cytoplasmic protein, associates with the microtubular cytoskeleton. Reviewed
Nadano, D., Nakayama, J., Matsuzawa, S., Sato, T., Matsuda, T., and Fukuda, M. N.
Biochem. J. Vol. 364 ( 3 ) page： 669-677 2002.6
Structural change of ribosomes during apoptosis: degradation and externalization of ribosomal proteins in doxorubicin-treated Jurkat cells. Reviewed
Nishida, J., Shiratsuchi, A., Nadano, D., Sato, T., and Nakanishi, Y.
J. Biochem. Vol. 131 ( 3 ) page： 485-493 2002.3
Significant differences between mouse and human trophinins are revealed by their expression patterns and targeted disruption of mouse trophinin gene. Reviewed
Nadano, D., Sugihara, K., Paria, B. C., Saburi, S., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Nakayama, J., and Fukuda, M. N.
Biol. Reprod. Vol. 66 ( 2 ) page： 313-321 2002.2
Electrophoretic characterization of ribosomal subunits and proteins in apoptosis. Specific down-regulation of S11 in human breast carcinoma treated with staurosporine. Reviewed
Nadano, D., Aoki, C., Yoshinaka, T., Irie, S., and Sato, T.
Biochemistry Vol. 40 ( 50 ) page： 15184-15193 2001.12
The trophinin gene encodes a novel group of MAGE proteins, Magphinins, and regulates cell proliferation during gametogenesis in the mouse. Reviewed
Saburi, S., Nadano, D., Akama, T. O., Hirama, K., Yamanouchi, K., Naito, K., Tojo, H., Tachi, C., and Fukuda, M. N.
J. Biol. Chem. Vol. 276 ( 52 ) page： 49378-49389 2001.12
14-3-3 is involved in p75 neurotrophin receptor (p75NTR)-mediated signal transduction. Reviewed
Kimura, M. T., Irie S., Shoji-Hoshino S., Mukai J., Nadano D., Oshimura M., and Sato, T.
J. Biol. Chem. Vol. 276 ( 20 ) page： 17291-17300 2001.5
Trophinin expression in the mouse uterus coincides with implantation and is hormonally regulated but not induced by implanting blastocysts. Reviewed
"Suzuki, N., Nadano, D., Paria, B. C., Kupriyanov, S., Sugihara, K., and Fukuda, M. N."
Endocrinology Vol. 141 ( 11 ) page： 4247-4254 2000.11
Preparation and Characterization of Antibodies against Human Ribosomal Proteins. Heterogeneous Expression of S11 and S30 in a Panel of Human Cancer Cell Lines. Reviewed
Nadano, D., Ishihara, G., Aoki, C., Yoshinaka, T., Irie, S., and Sato, T.
Jpn. J. Cancer Res. Vol. 91 page： 802-810 2000.8
NADE, p75NTR-associated cell death executor, is involved in the common neurotrophin receptor p75NTR-mediated signal transduction. Reviewed
Mukai, J., Hachiya, T., Hoshino, S., Kimura, M., Nadano, D., Suvanto, P., Hanaoka, T., Li, Y., Irie, S., and Sato, T.
J. Biol. Chem. Vol. 275 ( 23 ) page： 17566-17570 2000.6
Caspase-3-dependent and -independent degradation of 28 S ribosomal RNA may be involved in the inhibition of protein synthesis during apoptosis initiated by death receptor engagement. Reviewed
Nadano, D., and Sato, T.
J. Biol. Chem. Vol. 275 ( 18 ) page： 13967-13973 2000.5
Anthropological applications of genetic polymorphisms detected in human urine: a review of Japanses studies Invited
Yasuda, T., Nadano, D., Takeshita, H., Iida, R., and Kishi, K.
Rivista di Antropologia Vol. 75 page： 145-155 1997
Alpha-2-HS-glycoprotein (AHSG) polymorphism in semen andsaliva. Reviewed
Yasuda, T., Takeshita, H., Tsubota, E., Sawazaki, K., Iida, R., Nadano,D., and Kishi, K.
Electrophoresis Vol. 17 ( 4 ) page： 793-796 1996
pH gradient electrophoresis of basic ribonucleases in sealed slabpolyacrylamide gels: detection and inhibition of enzyme activity in thegel. Reviewed
Nadano, D., Yasuda, T., Sawazaki, K., Takeshita, H., and Kishi, K.
Electrophoresis Vol. 17 ( 1 ) page： 104-109 1996
A new individualization marker of sweat: deoxyribonuclease I (DNase I) polymorphism. Reviewed
Yasuda, T., Takeshita, H., Sawazaki, K., Nadano, D., Iida, R., Miyahara, S., and Kishi, K.
J. Forensic Sci. Vol. 41 ( 5 ) page： 862-864 1996
Purification of mammalian ribonuclease using immobilized human ribonuclease inhibitor. Reviewed
Nadano, D., Yasuda, T., Takeshita, H., Sawazaki, K.. and Kishi, K.
Protein Expr. Purif. Vol. 7 ( 2 ) page： 167-172 1996
Ribonucleaseinhibitors in human blood: comparative studies on the inhibitors detectedin erythrocytes, platelets, mononuclear leukocytes and granulocytes. Reviewed
Nadano, D., Yasuda, T., Takeshita, H., and Kishi, K.
Int. J. Biochem. Cell Biol. Vol. 27 ( 9 ) page： 971-979 1995
The molecular basis for genetic polymorphism of humandeoxyribonuclease I: identification of the nucleotide substitution thatgenerates the fourth allele. Reviewed
Yasuda, T., Nadano, D., Takeshita, H., Tenjo, E., Sawazaki, K., Ootani, M., and Kishi, K.
FEBS Lett. Vol. 359 ( 2-3 ) page： 211-214 1995
Molecular analysis of the third allele of human deoxyribonuclease I polymorphism. Reviewed
Yasuda, T., Nadano, D., Takeshita, H., Tenjo, E., and Kishi, K.
Ann. Hum. Genet. Vol. 59 ( 2 ) page： 139-147 1995
Deoxyribonuclease I from rat urine: affinity purification, characterization, and immunochemical studies. Reviewed
Takeshita, H., Yasuda, T., Nadano, D., Iida, R., and Kishi, K.
J. Biochem. Vol. 118 ( 5 ) page： 932-938 1995
Chromosomal assignment of the human deoxyribonuclease Igene, DNASE1 (DNL1), to band 16p13.3 using the polymerase chain reaction. Reviewed
Yasuda, T., Nadano, D., Iida, R., Takeshita, H., Lane, S.A., Callen, D.F., and Kishi, K.
Cytogen. Cell Genet. Vol. 70 ( 3-4 ) page： 221-223 1995
Activity staining of mammalian ribonuclease inhibitors after electrophoresis in sealedvertical slab polyacrylamide gels. Reviewed
Nadano, D., Yasuda, T., Takeshita, H., and Kishi, K.
Anal. Biochem. Vol. 227 ( 1 ) page： 210-215 1995
Genotyping of human deoxyribonuclease Ipolymorphism by the polymerase chain reaction. Reviewed
Yasuda, T., Nadano, D., Tenjo, E., Takeshita, H., Sawazaki, K., Nakanaga, M., and Kishi K.
Electrophoresis Vol. 16 ( 10 ) page： 1889-1893 1995
Correlation of alpha-1-antitrypsin (PI)polymorphism between blood and semen. Reviewed
Sawazaki, K., Takeshita, H., Tenjo, E., Nadano, D., Yasuda, T., Iida, R., and Kishi, K.
Jpn. J. Legal Med. Vol. 49 ( 2 ) page： 65-69 1995
Detection of deoxyribonucleases I and II (DNases I and II) activities in reproductive organs of male rabbits. Reviewed
Takeshita, H., Yasuda, T., Nadano, D., Tenjo, E., Sawazaki, K., Iida, R., and Kishi , K.
Int. J. Biochem. Vol. 26 ( 8 ) page： 1025-1031 1994
Purification and characterization of human brain ribonuclease inhibitor. Reviewed
Nadano, D., Yasuda, T., Takeshita, H., Uchide, K., and Kishi, K.
Arch. Biochem. Biophys Vol. 312 ( 2 ) page： 421-428 1994
Genetically polymorphic alpha-L-fucosidase(FUCA1) isozymes detected in blood plasma. Reviewed
Takeshita, H., Yasuda, T., Nadano, D., Iida, R., Nakanaga, M., Tenjo, E., Sawazaki, K. and Kishi K.
Hum. Genet. Vol. 94 ( 3 ) page： 224-230 1994
Use of antibodies against synthetic peptides for analysis of molecular multiplicity: humandeoxyribonuclease I. Reviewed
Yasuda, T., Nadano, D., Takeshita, H., and Kishi, K.
Electrophoresis Vol. 15 ( 2 ) page： 260-264 1994
Salivary deoxyribonuclease I polymorphism separated by polyacrylamide gel-isoelectric focusing and detected by the dried agarose film overlay method. Reviewed
Tenjo, E., Sawazaki, K., Yasuda, T., Nadano, D., Takeshita, H., Iida, R., and Kishi, K.
Electrophoresis Vol. 14 ( 10 ) 1993
Postmortem concentrations of thiopental in tissues: a sudden death case. Reviewed
Yasuda, T., Yamaba, T., Sawazaki, K., Masuyama, F., Nadano, D., Takeshita, H., and Kishi, K.
Int. J. Legal Med. Vol. 105 ( 4 ) page： 239-241 1993
Measurement of deoxyribonuclease I activity in human tissues and body fluids by a single radial enzyme-diffusion method. Reviewed
Nadano, D., Yasuda, T., and Kishi, K.
Clin. Chem. Vol. 39 ( 3 ) page： 448-452 1993
Activity measurement and multiplicity detection of human secretory-type ribonuclease based on polycytidylic acid/ethidium bromide fluorescence. Reviewed
Nadano, D., Yasuda, T., Sawazaki, K., Takeshita, H., and Kishi , K.
Anal. Biochem. Vol. 212 ( 1 ) page： 111-116 1993
Survey of the association of deoxyribonuclease I polymorphism with disease. Reviewed
Yasuda, T., Nadano, D., Tenjo, E., Takeshita, H., Nakanaga, M., and Kishi, K.
Hum. Hered. Vol. 43 ( 4 ) page： 205-208 1993
Deoxyribonuclease I (DNase I) typing from semen stains: low enzyme activity in vaginal fluids does not interfere with seminal DNase I typing from mixture stains. Reviewed
Sawazaki, K., Yasuda, T., Nadano, D., Iida, R., Takeshita, H., Uchide, K., and Kishi, K.
J. Forensic Sci. Vol. 38 ( 5 ) page： 1051-1062 1993
Human seminal deoxyribonuclease I (DNase I): purification, enzymological and immunological characterization and origin. Reviewed
Yasuda, T., Sawazaki, K., Nadano, D., Takeshita, H., Nakanaga, M., and Kishi, K.
Clin. Chim. Acta Vol. 218 ( 1 ) page： 5-16 1993
Kappa marker typing with high-performance liquid chromatography: identification of kappa marker specific tryptic peptide from the kappa light chain ofimmunoglobulin G. Reviewed
Iida, R., Yasuda, T., Nadano, D., Takeshita, H., and Kishi, K.
J. Chromatogr. Vol. 622 ( 1 ) page： 9-12 1993
Two distinct secretory ribonucleases from human cerebrum: purification, characterization and relationships to other ribonucleases. Reviewed
Yasuda, T., Nadano, D., Takeshita, H., and Kishi, K.
Biochem. J. Vol. 296 ( 3 ) page： 617-625 1993
Specific identification of human ribonucleases by antibodies produced against two synthetic peptides corresponding to the N- and C-terminal amino-acid sequences of human urinary secretory-type ribonuclease. Reviewed
Yasuda, T., Nadano, D., Tanaka, Y., and Kishi, K.
Biochim. Biophys. Acta Vol. 1121 ( 3 ) page： 331-334 1992
Genetic polymorphism of human deoxyribonuclease II (DNase II): low activity levels in urine and leukocytes are due to an autosomal recessive allele. Reviewed
Yasuda, T., Nadano, D., Sawazaki, K., and Kishi, K.
Ann. Hum. Genet. Vol. 56 ( 1 ) page： 1-10 1992
The zymogram method for detection of ribonucleases after isoelectric focusing:analysis of multiple forms of human, bovine, and microbial enzymes. Reviewed
Yasuda, T., Nadano, D., Tenjo, E., Takeshita, H., and Kishi, K.
Anal. Biochem. Vol. 206 ( 1 ) page： 172-177 1992
Transferrin (TF) typing from semen stains using isoelectric focusing andimmunoblotting: correlation of TF types among blood, semen, urine, andvaginal secretion. Reviewed
Sawazaki, K., Yasuda, T., Nadano, D., Iida, R., and Kishi, K.
J. Forensic Sci. Vol. 37 ( 6 ) page： 1514-1524 1992
Human urine deoxyribonuclease II (DNase II) isoenzymes: a novel immunoaffinity purification, biochemical multiplicity, genetic heterogeneity and broad distribution among tissues and body fluids. Reviewed
Yasuda, T., Nadano, D., Awazu, S., and Kishi, K.
Biochim. Biophys. Acta Vol. 1119 ( 2 ) page： 185-193 1992
A new individualization marker of semen: deoxyribonuclease I (DNase I) polymorphism. Reviewed
Sawazaki, K., Yasuda, T., Nadano, D., Tenjo, E., Iida, R., Takeshita, H., and Kishi K.
Forensic Sci. Int. Vol. 57 ( 1 ) page： 39-44 1992
Discovery of a genetic polymorphism of human plasma protein C inhibitor(PCI): genetic survey utilizing isoelectric focusing followed byimmunoblotting, immunological and biochemical characterization. Reviewed
Yasuda, T., Nadano, D., Iida, R., Tanaka, Y., Nakanaga, M., and Kishi, K.
Hum. Genet. Vol. 89 ( 3 ) page： 265-269 1992
Transferrin polymorphisms in Japanese populations: north-southcline in the distribution of the TF*C2 allele. Reviewed
Nakanaga, M., Yasuda, T., Tenjo, E., Nadano, D., Fujiki, N., and Kishi, K.
Hum. Biol. Vol. 63 ( 2 ) page： 187-195 1991
Purification and characterization of genetically polymorphic deoxyribonuclease I from human kidney. Reviewed
Nadano, D., Yasuda, T., and Kishi K.
J. Biochem. Vol. 110 ( 3 ) page： 321-323 1991
Genetic polymorphism of human glutamateoxaloacetate transaminase (GOT1) detected using isoelectric focusing and asensitive and positive staining method. Reviewed
Nakanaga, M., Nadano, D., Yasuda, T., Tenjo, E., Sawazaki, K., Iida, R., Fujiki, N., and Kishi, K.
Int. J. Legal Med. Vol. 104 ( 3 ) page： 121-122 1991
Noveldetection method for human protein C inhibitor (PCI) using immunoblottingwith antibodies specific to synthetic peptides corresponding to theN-terminal and C-terminal amino acid sequences of human PCI. Reviewed
Yasuda, T., Nadano, D., Tanaka, Y., Sawazaki, K., and Kishi, K.
Biochem. Int. Vol. 25 ( 3 ) page： 553-560 1991
Transferrin subtyping of bloodstains and semen using isoelectricfocusing and immunoblotting. Reviewed
Yamaba, T., Sawazaki, K., Yasuda, T., Nadano, D., Iida, R., and Kishi, K.
Forensic Sci. Int. Vol. 50 ( 2 ) page： 187-194 1991
Isolation of H and Leb active human salivary mucins and structures of their sugar chains with the H and Ley (Y)determinants. Reviewed
Nadano, D., and Mukoyama, H.
Jpn. J. Legal Med. Vol. 44 ( 4 ) page： 259-266 1990
Practical utility of an antibody specific to a synthetic peptidecorresponding to the N-terminal amino acid sequence of human urine DNase Ifor genetic analysis of human DNase I isozymes. Reviewed
Yasuda, T., Nadano, D., Tanaka, Y., Sato, W., Nakanaga, M., and Kishi, K.
Biochem. Int. Vol. 22 ( 4 ) page： 699-705 1990
Intensification ofperoxidase-diaminobenzidine staining using gold-sulfide-silver: a rapid andhighly sensitive method for visualization in immunoblotting. Reviewed
Iida, R., Yasuda, T,. Nadano, D., and Kishi, K.
Electrophoresis Vol. 11 ( 10 ) page： 852-855 1990
Isolation and structure of a novel deaminated neuraminic acid containing oligosaccharide chain present in rainbow trout egg polysialoglycoprotein. Reviewed
Iwasaki, M., Inoue, S., Nadano, D., and Inoue, Y.
Biochemistry Vol. 26 ( 5 ) page： 1452-1457 1987
A naturally occurring deaminated neuraminic acid,3-deoxy-D-glycero-D-galactononulosonic acid (KDN). Its unique occurrence at the nonreducing ends of oligosialyl chains in polysialoglycoprotein of rainbow trout eggs. Reviewed
Nadano, D., Iwasaki, M., Endo, S., Kitajima, K., Inoue, S., and Inoue, Y.
J. Biol. Chem. Vol. 261 ( 25 ) page： 11550-11557 1986
ふぞろいな翻訳系：哺乳類リボソーム不均一性因子による新規翻訳制御機構の統括的理解
Grant number：21K05379 2021.4 - 2025.3
科学研究費助成事業 基盤研究(C)
灘野 大太
Authorship：Principal investigator Grant type：Competitive
Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）
真核細胞におけるmRNAの転写後輸送、リボソーム生合成およびリボソームでの翻訳は往々にして別々に解析されてきた。ただ細胞内で必要なmRNAが転写されたとしても、mRNAを核外へさらにリボソームに輸送することならびに必要な量のリボソームを作り細胞質へ輸送することの両者が整う必要があり、加えて翻訳においてリボソームが効率よく機能しなければ、細胞にとって非効率的なばかりでなく、不具合の程度によっては生存能力に深刻な影響を与えうる。この相互のバランスの解明を本研究において進める。
研究代表者らはリボソームに含まれることで不均一性を担うタンパク質すなわち不均一性因子の同定および機能解析を独自に進めた。この過程において、細胞質においてリボソームに結合しつつmRNA核外輸送やリボソーム生合成との関与を示唆する不均一性因子が明らかにされた。これらから、リボソームが積極的に不均一になることでリボソーム自身での適切な新生タンパク質合成に加えて、このタンパク質合成をバランスよく支えるためにmRNAの転写後輸送やリボソーム自身の生合成までも管理・制御していることが着想された。哺乳類リボソームの不均一性因子による包括的な転写後タンパク質合成制御という観点から「ふぞろいな翻訳系」という新たな研究カテゴリーの創造を目指して当グループで見出した不均一性因子の分析を進めた。特にリボソームに会合するRNA結合タンパク質を軸として、その結合RNAの配列を含め解析に着手した。翻訳系に着目していることから、細胞からのリボソーム、なかでも翻訳中のリボソームであるポリソーム（1本のmRNAにリボソームが多数結合している状態、ポリリボソームとも）を精製して分析する方向性、もう一つの方向性としてRNA結合タンパク質の精製からの進め方についても検討した。これら独立した方向性からより網羅的に解明を進め、また不安定かつ微量な標的RNAを効率よくかつ円滑に次世代配列解析に移行できるよう工夫することで当初目的としたリボソーム会合RNA結合タンパク質の解析が進められた。
「研究実績の概要」に記載したとおり本研究計画の重要な解析対象であるリボソームの構造不均一性因子およびその関連因子・事項について分析を行ったことからおおむね順調と判断した。
1年目は上記のとおりおおむね順調に推移したことから、今後とも研究計画にある研究目標の達成をめざず。
Studies of novel mechanisms regulating cell growth by functional cooperation between nuclear ribosome biogenesis and cytoplasmic translation
Grant number：17K07751 2017.4 - 2020.3
Grant-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(C)
Nadano Daita
Authorship：Principal investigator Grant type：Competitive
Grant amount：\4810000 （ Direct Cost: \3700000 、 Indirect Cost：\1110000 ）
To study the mechanisms regulating cell growth by functional cooperation between nuclear ribosome biogenesis and cytoplasmic translation, we comprehensively analyzed the protein constituents of mammalian ribosomes. Serbp1, known as an mRNA-binding protein, was identified to be associated with the ribosomal small subunit in an mRNA-independent manner. Microscopic and biochemical analyses of Serbp1 indicated that this protein was mainly located in the nucleolus, a place where ribosome biogenesis occurs, within the nucleus and included in cytoplasmic polysomes including translating ribosomes. In addition, we identified and analyzed the novel proteins potentially involved in the mechanisms.
哺乳類精巣リボソームの構造不均一性がもたらす新規翻訳制御メカニズムの包括的解明
2013.4 - 2016.3
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
細胞自己複製および翻訳を共に促進する新規特性タンパク質の筋肉老化予防への応用
2009.4 - 2012.3
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
退縮初期の乳および乳腺からの新規生体防御因子のプロテオミクス的同定と機能解析
2006.4 - 2008.3
科学研究費補助金 基盤研究（Ｃ）（一般）
Authorship：Principal investigator
科研費
First Year Seminar
2023
Advanced Molecular Cell Biology 2
2021
Biochemistry IV
2021
Molecular Cell Biology 1
2021
Biochemistry 1
2021
Molecular Cell Biology 1
2020
Biochemistry 1
2020
修士論文研究４
2020
修士論文研究３
2020
修士論文研究２
2020
修士論文研究１
2020
生命農学演習４
2020
生命農学演習３
2020
生命農学演習２
2020
生命農学演習１
2020
分子細胞生物学特論２
2020
